MD16G1032000.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008821
Physical Location & Seq
Forward (+)
2297057 .. 2298017
961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1032000.v1.1.491

Sequence Viewer

Length: 480 bp
ATGCAGAAGAAGGCAAAAGGAATAATGGCTTCAGTTTCAGCAGCGTGGTTGCCGTACCTTTCCACTCGAGCCTCCACACCTTATGCTTCTCTCACATTCCTCACTAGCTCCACAACTAGGAAGAGTTCCGTGTCTCTCAGCGGTGGCGCCGCTGCTCGTTCCCCGTTGCTTCACTGCTCCTTCATCTCTTTCACCTCTTTAGCTTCCACTTCCGTCTTCTTAGGTTTATCTTTGGGCTTGGATTCGAGCTCTAGTGTGGAGGTTAGAAGAAGAAGAGGCTGTGGCTTAGTGGTGAGGACCGGGAAGGCTGCCCTGTGTCTAACCAAGAGAAGTAGGTCCAGGAAATCCTTGGTTCAAACTCATGGATTCCGTTGCCGCATGAGAACAACTGGCGGGAGAGCAATGATGAAGCGCCAACGTGCTAAGGGGCGGAAGGTCCTCTGCACGAAGAGCAATTCCAACTGTGGAAAACGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

160

Amino Acids

17.09

Weight (kDa)

11.88

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 119 - 148 8.1e-08 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AciI CCGC 5 cut(s) 141, 150, 376, 393, 430
AcuI CTGAAG 1 cut(s) 15
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 1 cut(s) 356
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 3 cut(s) 108, 203, 249
AluI AGCT 3 cut(s) 108, 203, 249
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 66
ApeKI GCWGC 3 cut(s) 41, 152, 308
AspLEI GCGC 2 cut(s) 149, 414
AspS9I GGNCC 3 cut(s) 297, 336, 436
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 2 cut(s) 184, 304
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 3 cut(s) 297, 336, 436
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 3 cut(s) 53, 139, 295
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 340
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 3 cut(s) 105, 117, 252
BfoI RGCGCY 2 cut(s) 150, 415
BisI GCNGC 5 cut(s) 42, 150, 153, 309, 376
BlsI GCNGC 5 cut(s) 43, 151, 154, 310, 377
Bme1390I CCNGG 2 cut(s) 301, 340
Bme18I GGWCC 3 cut(s) 297, 336, 436
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 3 cut(s) 297, 336, 436
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 301, 340
BpiI GAAGAC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 1 cut(s) 151
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 3 cut(s) 53, 139, 295
BsgI GTGCAG 1 cut(s) 427
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 251
BspACI CCGC 5 cut(s) 141, 150, 376, 393, 430
BspCNI CTCAG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 3 cut(s) 116, 268, 443
BstACI GRCGYC 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 137, 220, 286, 423
BstH2I RGCGCY 2 cut(s) 150, 415
BstHHI GCGC 2 cut(s) 149, 414
BstMAI GTCTC 1 cut(s) 138
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNI CCWGG 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 299, 338
BstV1I GCAGC 3 cut(s) 53, 139, 295
BstV2I GAAGAC 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 172
CfoI GCGC 2 cut(s) 149, 414
Cfr13I GGNCC 3 cut(s) 297, 336, 436
Csp6I GTAC 1 cut(s) 55
CviAII CATG 2 cut(s) 362, 379
CviJI RGCY 9 cut(s) 29, 71, 108, 203, 237, 249, 279, 285, 308
CviKI_1 RGCY 9 cut(s) 29, 71, 108, 203, 237, 249, 279, 285, 308
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 4 cut(s) 137, 220, 286, 423
DinI GGCGCC 1 cut(s) 148
Eam1104I CTCTTC 3 cut(s) 116, 268, 443
EarI CTCTTC 3 cut(s) 116, 268, 443
EciI GGCGGA 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 251
Eco47I GGWCC 3 cut(s) 297, 336, 436
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 251
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 2 cut(s) 365, 382
FaiI YATR 3 cut(s) 84, 363, 380
FatI CATG 2 cut(s) 361, 378
FauI CCCGC 1 cut(s) 386
Fnu4HI GCNGC 5 cut(s) 42, 150, 153, 309, 376
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 5 cut(s) 42, 150, 153, 309, 376
FspBI CTAG 3 cut(s) 105, 117, 252
GlaI GCGC 2 cut(s) 148, 413
GluI GCNGC 5 cut(s) 42, 150, 153, 309, 376
HaeII RGCGCY 2 cut(s) 150, 415
HapII CCGG 1 cut(s) 300
HhaI GCGC 2 cut(s) 149, 414
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 365, 382
Hin6I GCGC 2 cut(s) 147, 412
HinP1I GCGC 2 cut(s) 147, 412
HinfI GANTC 2 cut(s) 242, 366
HpaII CCGG 1 cut(s) 300
HphI GGTGA 2 cut(s) 184, 304
HpyAV CCTTC 4 cut(s) 4, 190, 298, 427
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4IV ACGT 2 cut(s) 418, 472
HpyCH4V TGCA 2 cut(s) 4, 444
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 4 cut(s) 137, 220, 286, 423
HpySE526I ACGT 2 cut(s) 418, 472
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 365, 382
HspAI GCGC 2 cut(s) 147, 412
KasI GGCGCC 1 cut(s) 146
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 113, 182
LpnPI CCDG 5 cut(s) 313, 325, 326, 352, 375
Lsp1109I GCAGC 3 cut(s) 53, 139, 295
MaeI CTAG 3 cut(s) 105, 117, 252
MaeII ACGT 2 cut(s) 418, 472
MboII GAAGA 7 cut(s) 19, 133, 208, 279, 282, 285, 460
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 1 cut(s) 454
Mly113I GGCGCC 1 cut(s) 147
MnlI CCTC 7 cut(s) 82, 110, 205, 253, 269, 288, 449
MspA1I CMGCKG 2 cut(s) 141, 152
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 301, 340
MvaI CCWGG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 450
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 301
NlaIII CATG 2 cut(s) 365, 382
NlaIV GGNNCC 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 242, 366
PfoI TCCNGGA 1 cut(s) 338
PkrI GCNGC 5 cut(s) 43, 151, 154, 310, 377
PluTI GGCGCC 1 cut(s) 150
PpuMI RGGWCCY 1 cut(s) 436
Psp124BI GAGCTC 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 338
PspGI CCWGG 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 3 cut(s) 297, 336, 436
PspPPI RGGWCCY 1 cut(s) 436
PspXI VCTCGAGB 1 cut(s) 66
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 443
SatI GCNGC 5 cut(s) 42, 150, 153, 309, 376
Sau96I GGNCC 3 cut(s) 297, 336, 436
ScrFI CCNGG 2 cut(s) 301, 340
SduI GDGCHC 1 cut(s) 251
SfoI GGCGCC 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 66
SinI GGWCC 3 cut(s) 297, 336, 436
SlaI CTCGAG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 1 cut(s) 454
SsiI CCGC 5 cut(s) 141, 150, 376, 393, 430
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 3 cut(s) 105, 117, 252
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 2 cut(s) 299, 338
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 464
TaiI ACGT 2 cut(s) 421, 475
TaqI TCGA 2 cut(s) 67, 245
TasI AATT 1 cut(s) 454
TauI GCSGC 2 cut(s) 152, 378
TfiI GAWTC 2 cut(s) 242, 366
TscAI CASTG 1 cut(s) 179
TseI GCWGC 3 cut(s) 41, 152, 308
TspDTI ATGAA 2 cut(s) 172, 422
TspGWI ACGGA 3 cut(s) 118, 202, 359
TspRI CASTG 1 cut(s) 179
VpaK11BI GGWCC 3 cut(s) 297, 336, 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 66
XspI CTAG 3 cut(s) 105, 117, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.