Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007492

Physical Location & Seq

Genomic Coordinates Forward (+)

43961637 .. 43962503

Gene Structure 867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1308600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 867 bp

ATGGAGGGAGATGAAGCAAGAGATTTGTTTGGAGCACAGTCCCATTTGTACAAGCATGTATTCAGCTTCATAACTTCTATGTCACTCAAGTGTGTAGTGCAGCTCGGCATACCGGACATAATTGACCGCCACGGACAACCCATTACTCTACCTGACTTGGTCACAGCACTTCAGATCCACCCGGCTAAAACCGGTAACGTGCACCGGCTCATGCGCCTCATGGTGCACTCCGGCTTCTTTGCACGAAAACAAGTTCCTAAAAATCATGTAGAAGCAGATGAAGGAGAAGAAGAGGCGTATGATCTCACACCGTCTTCTACGCTCCTCCTGAAAGACAAAGTACCCAGCTTGTCTTCGTTCGTTCTGGGGATGCTCGATCCAGCTTCCGCAGCACCGTGGCAGTTCCTCGGAAACTGGTTCCGAGGAACCGAGGTCACGCCTTTCGAGAGCGCTCATGGGATGGGGTTTTGGGAATACGCGGAAGGAAACCCCGAATTCAACAGTCTTTTCAATAAGGCAATGGCTAGTGATTCTGGAATGATGAACTTGGTGATTAGAGATTGCAAGCCAATCTTTGATGGGTTGAGTTCATTGGTTGATGTTGGTGGTGGGACTGGAAAAGTTGCTAGGATTCTCTGTGACGCTTTCCCTCAACTGAAATGCACAGTTCTTGAACTTCCACACGTTGTCGCTGATTTGCCAGACTGTGAAAATTTGAAGTTTGTTCGAGGAGACATGTTCCAGGGTATCCCTCCAGCGGATGCTGTTTTCCTCAAGGTAAGCAAATTTTCAACTTTTCAATTATCTTCTGTTTTGACTATATGTAATTGCATCAACTATTTAGCCTATCATAACAAGATGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0009058 GO:0009820 GO:0009821 GO:0009987 GO:0016740 GO:0016741 GO:0019438 GO:0030766 GO:0032259 GO:0034641 GO:0042802 GO:0042803 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0046983 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00945 map00945

Pfam Domains

Protein Families

Dimerisation Methyltransf_2

Protein Analysis

289

Amino Acids

31.83

Weight (kDa)

5.56

Isoelectric Point (pI)

31.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dimerisation	PF08100	18 - 112	9.6e-19	O-methyltransferase dimerisation domain
Methyltransf_2	PF00891	133 - 259	2.2e-27	O-methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007492)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g08410
malus_domestica	MD05G1308600.v1.1 MD05G1308800.v1.1
prunus_persica	Prupe.4G054200_v2.0.a1
pyrus_communis	pycom05g28530 pycom05g28540
rosa_chinensis	RchiOBHm_Chr5g0009461 RchiOBHm_Chr5g0009471
rosa_laevigata	RLG00000031664
rosa_multiflora	Rmu_sc0002711.1_g000015 Rmu_sc0002711.1_g000016
rosa_roxburghii	Rroxscaffold_1G00066660 Rroxscaffold_1G00067360
rosa_rugosa	Rorug04G0443300 Rorug04G0443300
rosa_samantha	Rh5AG074300 Rh5AG074400 Rh5BG070100 Rh5BG070200 Rh5CG082500 Rh5CG082600 Rh5DG070400 Rh5DG070500
rosa_wichuraiana	Rw5G006880 Rw5G006890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	479
AciI	CCGC	4 cut(s)	127, 387, 479, 758
AclWI	GGATC	2 cut(s)	169, 371
AcsI	RAATTY	3 cut(s)	494, 712, 785
AcuI	CTGAAG	1 cut(s)	155
AfaI	GTAC	2 cut(s)	50, 342
AfeI	AGCGCT	1 cut(s)	451
AfiI	CCNNNNNNNGG	1 cut(s)	757
AflIII	ACRYGT	2 cut(s)	682, 735
AgeI	ACCGGT	1 cut(s)	191
AgsI	TTSAA	6 cut(s)	499, 511, 674, 718, 792, 800
AjnI	CCWGG	1 cut(s)	741
AleI	CACNNNNGTG	1 cut(s)	88
AluBI	AGCT	4 cut(s)	66, 103, 348, 383
AluI	AGCT	4 cut(s)	66, 103, 348, 383
Alw21I	GWGCWC	3 cut(s)	37, 204, 228
Alw26I	GTCTC	1 cut(s)	726
Alw44I	GTGCAC	2 cut(s)	200, 224
AlwI	GGATC	2 cut(s)	169, 371
Aor51HI	AGCGCT	1 cut(s)	451
ApaLI	GTGCAC	2 cut(s)	200, 224
ApeKI	GCWGC	2 cut(s)	100, 389
ApoI	RAATTY	3 cut(s)	494, 712, 785
AsiGI	ACCGGT	1 cut(s)	191
AspLEI	GCGC	2 cut(s)	216, 452
AsuC2I	CCSGG	1 cut(s)	182
AsuHPI	GGTGA	1 cut(s)	562
BaeGI	GKGCMC	2 cut(s)	204, 228
BbsI	GAAGAC	2 cut(s)	306, 345
Bbv12I	GWGCWC	3 cut(s)	37, 204, 228
BbvI	GCAGC	2 cut(s)	112, 401
BccI	CCATC	3 cut(s)	454, 572, 853
BciT130I	CCWGG	1 cut(s)	743
BciVI	GTATCC	1 cut(s)	758
BcnI	CCSGG	1 cut(s)	182
BcoDI	GTCTC	1 cut(s)	726
BfaI	CTAG	2 cut(s)	525, 627
BfoI	RGCGCY	1 cut(s)	453
BfuI	GTATCC	1 cut(s)	758
BisI	GCNGC	2 cut(s)	101, 390
BlsI	GCNGC	2 cut(s)	102, 391
Bme1390I	CCNGG	2 cut(s)	182, 743
BmiI	GGNNCC	2 cut(s)	419, 427
BmrFI	CCNGG	2 cut(s)	182, 743
BmsI	GCATC	3 cut(s)	360, 751, 840
BpiI	GAAGAC	2 cut(s)	306, 345
BpmI	CTGGAG	1 cut(s)	738
BpuEI	CTTGAG	2 cut(s)	71, 758
BpuMI	CCSGG	1 cut(s)	182
BsaJI	CCNNGG	6 cut(s)	130, 395, 406, 421, 429, 742
BsaWI	WCCGGW	2 cut(s)	112, 191
Bsc4I	CCNNNNNNNGG	1 cut(s)	757
Bse118I	RCCGGY	2 cut(s)	191, 204
Bse1I	ACTGG	2 cut(s)	419, 619
Bse3DI	GCAATG	1 cut(s)	525
BseBI	CCWGG	1 cut(s)	743
BseDI	CCNNGG	6 cut(s)	130, 395, 406, 421, 429, 742
BseGI	GGATG	3 cut(s)	375, 465, 766
BseLI	CCNNNNNNNGG	1 cut(s)	757
BseMI	GCAATG	1 cut(s)	525
BseNI	ACTGG	2 cut(s)	419, 619
BseRI	GAGGAG	2 cut(s)	314, 744
BseSI	GKGCMC	2 cut(s)	204, 228
BseXI	GCAGC	2 cut(s)	112, 401
BseYI	CCCAGC	1 cut(s)	344
BsgI	GTGCAG	1 cut(s)	119
Bsh1236I	CGCG	1 cut(s)	479
BshTI	ACCGGT	1 cut(s)	191
BsiHKAI	GWGCWC	3 cut(s)	37, 204, 228
BsiSI	CCGG	5 cut(s)	113, 182, 192, 205, 231
BslFI	GGGAC	2 cut(s)	25, 625
BslI	CCNNNNNNNGG	1 cut(s)	757
BsmAI	GTCTC	1 cut(s)	726
BsmFI	GGGAC	2 cut(s)	25, 625
Bsp1286I	GDGCHC	3 cut(s)	37, 204, 228
Bsp1407I	TGTACA	1 cut(s)	48
Bsp143I	GATC	3 cut(s)	174, 301, 376
BspACI	CCGC	4 cut(s)	127, 387, 479, 758
BspFNI	CGCG	1 cut(s)	479
BspLI	GGNNCC	2 cut(s)	419, 427
BspPI	GGATC	2 cut(s)	169, 371
BsrDI	GCAATG	1 cut(s)	525
BsrFI	RCCGGY	2 cut(s)	191, 204
BsrGI	TGTACA	1 cut(s)	48
BsrI	ACTGG	2 cut(s)	419, 619
BssAI	RCCGGY	2 cut(s)	191, 204
BssECI	CCNNGG	6 cut(s)	130, 395, 406, 421, 429, 742
BssMI	GATC	3 cut(s)	174, 301, 376
Bst2UI	CCWGG	1 cut(s)	743
Bst4CI	ACNGT	6 cut(s)	39, 312, 396, 503, 667, 707
Bst6I	CTCTTC	1 cut(s)	285
BstAUI	TGTACA	1 cut(s)	48
BstC8I	GCNNGC	1 cut(s)	566
BstDSI	CCRYGG	2 cut(s)	130, 395
BstF5I	GGATG	3 cut(s)	375, 465, 766
BstFNI	CGCG	1 cut(s)	479
BstH2I	RGCGCY	1 cut(s)	453
BstHHI	GCGC	2 cut(s)	216, 452
BstKTI	GATC	3 cut(s)	177, 304, 379
BstMAI	GTCTC	1 cut(s)	726
BstMBI	GATC	3 cut(s)	174, 301, 376
BstMWI	GCNNNNNNNGC	1 cut(s)	389
BstNI	CCWGG	1 cut(s)	743
BstNSI	RCATGY	2 cut(s)	59, 739
BstSCI	CCNGG	2 cut(s)	180, 741
BstSLI	GKGCMC	2 cut(s)	204, 228
BstUI	CGCG	1 cut(s)	479
BstV1I	GCAGC	2 cut(s)	112, 401
BstV2I	GAAGAC	2 cut(s)	306, 345
BstX2I	RGATCY	1 cut(s)	174
BstYI	RGATCY	1 cut(s)	174
BsuI	GTATCC	1 cut(s)	758
BtgI	CCRYGG	2 cut(s)	130, 395
BtsCI	GGATG	3 cut(s)	375, 465, 766
Cac8I	GCNNGC	1 cut(s)	566
CfoI	GCGC	2 cut(s)	216, 452
Cfr10I	RCCGGY	2 cut(s)	191, 204
CseI	GACGC	1 cut(s)	650
Csp6I	GTAC	2 cut(s)	49, 341
CspAI	ACCGGT	1 cut(s)	191
CviAII	CATG	6 cut(s)	56, 211, 220, 266, 455, 736
CviQI	GTAC	2 cut(s)	49, 341
DpnI	GATC	3 cut(s)	176, 303, 378
DpnII	GATC	3 cut(s)	174, 301, 376
Eam1104I	CTCTTC	1 cut(s)	285
EarI	CTCTTC	1 cut(s)	285
Eco47III	AGCGCT	1 cut(s)	451
Eco57I	CTGAAG	1 cut(s)	155
EcoRI	GAATTC	1 cut(s)	494
EcoRII	CCWGG	1 cut(s)	741
FaeI	CATG	6 cut(s)	59, 214, 223, 269, 458, 739
FalI	AAGNNNNNCTT	2 cut(s)	557, 589
FaqI	GGGAC	2 cut(s)	25, 625
FatI	CATG	6 cut(s)	55, 210, 219, 265, 454, 735
Fnu4HI	GCNGC	2 cut(s)	101, 390
FokI	GGATG	3 cut(s)	382, 472, 773
Fsp4HI	GCNGC	2 cut(s)	101, 390
FspBI	CTAG	2 cut(s)	525, 627
GlaI	GCGC	2 cut(s)	215, 451
GluI	GCNGC	2 cut(s)	101, 390
GsaI	CCCAGC	1 cut(s)	348
GsuI	CTGGAG	1 cut(s)	738
HaeII	RGCGCY	1 cut(s)	453
HapII	CCGG	5 cut(s)	113, 182, 192, 205, 231
HgaI	GACGC	1 cut(s)	650
HhaI	GCGC	2 cut(s)	216, 452
Hin1II	CATG	6 cut(s)	59, 214, 223, 269, 458, 739
Hin6I	GCGC	2 cut(s)	214, 450
HinP1I	GCGC	2 cut(s)	214, 450
HinfI	GANTC	2 cut(s)	530, 631
HpaII	CCGG	5 cut(s)	113, 182, 192, 205, 231
HphI	GGTGA	1 cut(s)	562
Hpy166II	GTNNAC	2 cut(s)	202, 226
Hpy188I	TCNGA	3 cut(s)	174, 410, 422
Hpy188III	TCNNGA	4 cut(s)	328, 445, 534, 671
Hpy8I	GTNNAC	2 cut(s)	202, 226
HpyAV	CCTTC	2 cut(s)	275, 476
HpyCH4III	ACNGT	6 cut(s)	39, 312, 396, 503, 667, 707
HpyCH4IV	ACGT	2 cut(s)	198, 684
HpyCH4V	TGCA	7 cut(s)	100, 202, 226, 242, 564, 663, 831
HpyF10VI	GCNNNNNNNGC	1 cut(s)	389
HpySE526I	ACGT	2 cut(s)	198, 684
Hsp92II	CATG	6 cut(s)	59, 214, 223, 269, 458, 739
HspAI	GCGC	2 cut(s)	214, 450
Kzo9I	GATC	3 cut(s)	174, 301, 376
LmnI	GCTCC	2 cut(s)	32, 327
Lsp1109I	GCAGC	2 cut(s)	112, 401
LweI	GCATC	3 cut(s)	360, 751, 840
MaeI	CTAG	2 cut(s)	525, 627
MaeII	ACGT	2 cut(s)	198, 684
MaeIII	GTNAC	5 cut(s)	81, 160, 194, 433, 638
MalI	GATC	3 cut(s)	176, 303, 378
MboI	GATC	3 cut(s)	174, 301, 376
MboII	GAAGA	5 cut(s)	299, 302, 306, 345, 798
MflI	RGATCY	1 cut(s)	174
MhlI	GDGCHC	3 cut(s)	37, 204, 228
MluCI	AATT	6 cut(s)	120, 494, 712, 785, 800, 826
MslI	CAYNNNNRTG	1 cut(s)	88
MspA1I	CMGCKG	1 cut(s)	758
MspI	CCGG	5 cut(s)	113, 182, 192, 205, 231
MspR9I	CCNGG	2 cut(s)	182, 743
MvaI	CCWGG	1 cut(s)	743
MvnI	CGCG	1 cut(s)	479
MwoI	GCNNNNNNNGC	1 cut(s)	389
NciI	CCSGG	1 cut(s)	182
NdeII	GATC	3 cut(s)	174, 301, 376
NlaIII	CATG	6 cut(s)	59, 214, 223, 269, 458, 739
NlaIV	GGNNCC	2 cut(s)	419, 427
NmeAIII	GCCGAG	1 cut(s)	84
NmuCI	GTSAC	4 cut(s)	81, 160, 433, 638
NspI	RCATGY	2 cut(s)	59, 739
OliI	CACNNNNGTG	1 cut(s)	88
PciI	ACATGT	1 cut(s)	735
PfeI	GAWTC	2 cut(s)	530, 631
PflFI	GACNNNGTC	1 cut(s)	158
PinAI	ACCGGT	1 cut(s)	191
PkrI	GCNGC	2 cut(s)	102, 391
PscI	ACATGT	1 cut(s)	735
Psp6I	CCWGG	1 cut(s)	741
PspFI	CCCAGC	1 cut(s)	344
PspGI	CCWGG	1 cut(s)	741
PspN4I	GGNNCC	2 cut(s)	419, 427
PsuI	RGATCY	1 cut(s)	174
PsyI	GACNNNGTC	1 cut(s)	158
RsaI	GTAC	2 cut(s)	50, 342
RsaNI	GTAC	2 cut(s)	49, 341
RseI	CAYNNNNRTG	1 cut(s)	88
SatI	GCNGC	2 cut(s)	101, 390
Sau3AI	GATC	3 cut(s)	174, 301, 376
ScrFI	CCNGG	2 cut(s)	182, 743
SduI	GDGCHC	3 cut(s)	37, 204, 228
SetI	ASST	9 cut(s)	68, 105, 154, 201, 350, 385, 435, 687, 780
SfaNI	GCATC	3 cut(s)	360, 751, 840
SmiMI	CAYNNNNRTG	1 cut(s)	88
SmlI	CTYRAG	2 cut(s)	86, 773
SmoI	CTYRAG	2 cut(s)	86, 773
Sse9I	AATT	6 cut(s)	120, 494, 712, 785, 800, 826
SsiI	CCGC	4 cut(s)	127, 387, 479, 758
SspMI	CTAG	2 cut(s)	525, 627
StyD4I	CCNGG	2 cut(s)	180, 741
TaaI	ACNGT	6 cut(s)	39, 312, 396, 503, 667, 707
TaiI	ACGT	2 cut(s)	201, 687
TaqI	TCGA	3 cut(s)	375, 444, 727
TasI	AATT	6 cut(s)	120, 494, 712, 785, 800, 826
TatI	WGTACW	1 cut(s)	48
TfiI	GAWTC	2 cut(s)	530, 631
TseFI	GTSAC	4 cut(s)	81, 160, 433, 638
TseI	GCWGC	2 cut(s)	100, 389
Tsp45I	GTSAC	4 cut(s)	81, 160, 433, 638
TspDTI	ATGAA	5 cut(s)	27, 58, 294, 557, 579
TspGWI	ACGGA	1 cut(s)	147
Tth111I	GACNNNGTC	1 cut(s)	158
VneI	GTGCAC	2 cut(s)	200, 224
XapI	RAATTY	3 cut(s)	494, 712, 785
XceI	RCATGY	2 cut(s)	59, 739
XspI	CTAG	2 cut(s)	525, 627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.