MD05G1316100.v1.1

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009422
Physical Location & Seq
Reverse (-)
44530401 .. 44534316
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1316100.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGAGCAGCGGTCAAGGTGGACCCCACTCCCTGGCCTTCCGGGTGATGAGGCTGTGCAAGCCCTCATCCCAGGTGGACCCCATTCCCCTCCTTCTCGACCCCGCCGATCTCGTCGTCGGCGAGGACGTTTTCGACGACCCAATGGCAGCCGCGCAACTCCCCCGCCTCCTCGACGTCCACGACTCCTTGAACCCCTCCTCCTCCGCCTCCTCTGACTCCTCGGATCTCACCTACCGCACCCGGTTCCTCCTCCACCACCCCTCCGACTCCATTGGCCTCTCTGGCCTCCTCGTCCTCCCCCAAGCCTTCGGAGCAATATACTTAGGGGAGACATTTTGTAGCTATATTAGCATCAATAACAGCTCCAATTTCGAAGTCAAGGACATTATAATCAAGTCGGAAATGCAAACAGAAAGGCAGAGACTTGTGCTGTTGGACACGTCGAATTCGCCGGTTGAATCCATACGTGCAGGCGGCCGTTATGACTTCATTGTTGAACATGATGTCAAGGAACTCGGAGCTCACACGTTGGTTTGCACTGCATTGTACTATGACGGTGATGGTGAGCGTAAATATCTACCACAGTTCTTCAAATTCATTGTTTCCAATCCGCTTTCTGTCAGGACGAAGGTCCTTGTTATAAAGGAAATCACGCTTTTAGAAGCTTGCATCGAAAACCATACAAAGTCAAATCTTTTTATGGAACAAGTTGAGTTTGAGCCCGCTCAGCATTGGAGTGCAAAAATCCTAAAATCTGACGAACACCATTCCGAAAAGAATTCTCAAACAAGAGAGATATTTAAGCCACCAATTCTCATCGAATCCGGTGGAGGAATTCATAACTATCTTTTTCAGTTAACATCACATGGTTCAACCCAAGGGAAAGTTGAGGGAAGCAATATTCTTGGTAAACTTCAGATAACATGGCGAACTAATCTGGGCGAACCTGGTCGCCTACAAACACAGCAAATTATGGGAACTCCCATAACACGAAAGGATATTGAGTTGCATGTTGTGGAGGAGCCATCTGCCATCAAGTTGGAAAGACCCTTTTTGCTACACTTGAATTTAGCAAATGAGACAGATAAGGAACTCGGCCCTTTCGGAGTTTGTTTATCACAAGATGATTCACGTGAGGAGAATGTTGTTGTGATTAATGGTCTTCAAACAGTGGTTTTGCCGAGGGTTGAGGCGTTTAGTTCCACAAATTTCAATCTGAACCTGATTGCCACTAAACTTGGAGTTCAGAGGATCACGGGCATTACTGTGTTTAACATAAGAGAGAAAAAGTCATACGACCCTTTGCCAGATATGGAGATTTTTGTGGATCTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF974

Protein Analysis

446

Amino Acids

49.59

Weight (kDa)

5.3

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC13_N PF06159 9 - 201 4.1e-35 Trafficking protein particle complex subunit 13, N-terminal
TRAPPC13_M PF23647 205 - 324 3.8e-21 Trafficking protein particle complex subunit 13, middle domain
TRAPPC13_C PF23643 342 - 433 1e-10 Trafficking protein particle complex subunit 13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 389, 641
AatII GACGTC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 725
AccII CGCG 1 cut(s) 152
AciI CCGC 9 cut(s) 9, 102, 150, 163, 204, 235, 474, 611, 723
AclWI GGATC 3 cut(s) 231, 1259, 1335
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 6 cut(s) 445, 593, 778, 834, 1066, 1207
AcuI CTGAAG 1 cut(s) 899
AcvI CACGTG 1 cut(s) 1133
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 31
AflIII ACRYGT 2 cut(s) 438, 525
AgsI TTSAA 8 cut(s) 190, 458, 497, 592, 873, 1066, 1166, 1213
AjiI CACGTC 1 cut(s) 441
AjnI CCWGG 3 cut(s) 30, 69, 946
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 4 cut(s) 342, 363, 521, 665
AluI AGCT 4 cut(s) 342, 363, 521, 665
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 3 cut(s) 323, 415, 1073
AlwI GGATC 3 cut(s) 231, 1259, 1335
AoxI GGCC 5 cut(s) 33, 274, 283, 475, 1096
ApeKI GCWGC 2 cut(s) 6, 146
ApoI RAATTY 6 cut(s) 445, 593, 778, 834, 1066, 1207
AseI ATTAAT 1 cut(s) 1155
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 4 cut(s) 20, 76, 631, 1097
AsuC2I CCSGG 2 cut(s) 41, 241
AsuHPI GGTGA 4 cut(s) 55, 220, 569, 575
AsuII TTCGAA 1 cut(s) 372
AvaII GGWCC 3 cut(s) 20, 76, 631
BanII GRGCYC 2 cut(s) 523, 723
BbrPI CACGTG 1 cut(s) 1133
BbsI GAAGAC 1 cut(s) 1154
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 2 cut(s) 18, 158
BccI CCATC 3 cut(s) 554, 1033, 1040
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 3 cut(s) 32, 71, 948
BcnI CCSGG 2 cut(s) 41, 241
BcoDI GTCTC 3 cut(s) 323, 415, 1073
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 4 cut(s) 7, 147, 150, 475
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 4 cut(s) 8, 148, 151, 476
Bme1390I CCNGG 5 cut(s) 32, 41, 71, 241, 948
Bme18I GGWCC 3 cut(s) 20, 76, 631
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 4 cut(s) 20, 76, 631, 1097
BmiI GGNNCC 4 cut(s) 22, 78, 245, 1023
BmrFI CCNGG 5 cut(s) 32, 41, 71, 241, 948
BmsI GCATC 2 cut(s) 360, 678
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 1154
Bpu1102I GCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 372
BpuMI CCSGG 2 cut(s) 41, 241
BsaAI YACGTR 2 cut(s) 467, 1133
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 5 cut(s) 30, 69, 219, 877, 1181
BsaWI WCCGGW 1 cut(s) 824
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse118I RCCGGY 1 cut(s) 451
BseBI CCWGG 3 cut(s) 32, 71, 948
BseDI CCNNGG 5 cut(s) 30, 69, 219, 877, 1181
BseGI GGATG 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 740
BseRI GAGGAG 9 cut(s) 158, 187, 190, 199, 208, 239, 278, 1034, 1151
BseX3I CGGCCG 1 cut(s) 475
BseXI GCAGC 2 cut(s) 18, 158
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 5 cut(s) 35, 276, 285, 477, 1098
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 4 cut(s) 40, 241, 452, 825
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 3 cut(s) 323, 415, 1073
BsnI GGCC 5 cut(s) 35, 276, 285, 477, 1098
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 523, 723
Bsp143I GATC 4 cut(s) 106, 223, 1251, 1327
Bsp1720I GCTNAGC 1 cut(s) 726
BspACI CCGC 9 cut(s) 9, 102, 150, 163, 204, 235, 474, 611, 723
BspANI GGCC 5 cut(s) 35, 276, 285, 477, 1098
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 4 cut(s) 22, 78, 245, 1023
BspPI GGATC 3 cut(s) 231, 1259, 1335
BspT104I TTCGAA 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 5 cut(s) 30, 69, 219, 877, 1181
BssMI GATC 4 cut(s) 106, 223, 1251, 1327
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 877
Bst2UI CCWGG 3 cut(s) 32, 71, 948
Bst4CI ACNGT 4 cut(s) 557, 585, 1171, 1267
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 2 cut(s) 467, 1133
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 59, 472, 667, 723
BstDEI CTNAG 2 cut(s) 322, 726
BstF5I GGATG 1 cut(s) 65
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 4 cut(s) 109, 226, 1254, 1330
BstMAI GTCTC 3 cut(s) 323, 415, 1073
BstMBI GATC 4 cut(s) 106, 223, 1251, 1327
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 5 cut(s) 58, 282, 311, 348, 727
BstNI CCWGG 3 cut(s) 32, 71, 948
BstNSI RCATGY 1 cut(s) 1013
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 30, 39, 69, 239, 946
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 18, 158
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 2 cut(s) 223, 1327
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstYI RGATCY 2 cut(s) 223, 1327
BstZI CGGCCG 1 cut(s) 475
BsuRI GGCC 5 cut(s) 35, 276, 285, 477, 1098
BtrI CACGTC 1 cut(s) 441
BtsCI GGATG 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 537, 1176
Cac8I GCNNGC 4 cut(s) 59, 472, 667, 723
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 4 cut(s) 20, 76, 631, 1097
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 1 cut(s) 547
CspCI CAANNNNNGTGG 2 cut(s) 1219, 1254
CviAII CATG 4 cut(s) 500, 866, 924, 1010
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 2 cut(s) 322, 726
DpnI GATC 4 cut(s) 108, 225, 1253, 1329
DpnII GATC 4 cut(s) 106, 223, 1251, 1327
EaeI YGGCCR 1 cut(s) 475
EagI CGGCCG 1 cut(s) 475
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 521
EclXI CGGCCG 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 877
Eco24I GRGCYC 2 cut(s) 523, 723
Eco47I GGWCC 3 cut(s) 20, 76, 631
Eco52I CGGCCG 1 cut(s) 475
Eco53kI GAGCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 899
Eco72I CACGTG 1 cut(s) 1133
EcoICRI GAGCTC 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 631
EcoRI GAATTC 3 cut(s) 445, 778, 834
EcoRII CCWGG 3 cut(s) 30, 69, 946
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 2 cut(s) 523, 723
ErhI CCWWGG 1 cut(s) 877
FaeI CATG 4 cut(s) 503, 869, 927, 1013
FatI CATG 4 cut(s) 499, 865, 923, 1009
FauI CCCGC 3 cut(s) 109, 170, 730
Fnu4HI GCNGC 4 cut(s) 7, 147, 150, 475
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 2 cut(s) 523, 723
Fsp4HI GCNGC 4 cut(s) 7, 147, 150, 475
GlaI GCGC 1 cut(s) 153
GluI GCNGC 4 cut(s) 7, 147, 150, 475
HaeIII GGCC 5 cut(s) 35, 276, 285, 477, 1098
HapII CCGG 4 cut(s) 40, 241, 452, 825
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 4 cut(s) 503, 869, 927, 1013
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 6 cut(s) 182, 215, 266, 458, 821, 1127
HpaI GTTAAC 1 cut(s) 858
HpaII CCGG 4 cut(s) 40, 241, 452, 825
HphI GGTGA 4 cut(s) 55, 220, 569, 575
Hpy166II GTNNAC 5 cut(s) 20, 76, 178, 858, 911
Hpy188III TCNNGA 3 cut(s) 95, 622, 1331
Hpy8I GTNNAC 5 cut(s) 20, 76, 178, 858, 911
Hpy99I CGWCG 5 cut(s) 116, 119, 137, 176, 445
HpyAV CCTTC 4 cut(s) 46, 101, 316, 622
HpyCH4III ACNGT 4 cut(s) 557, 585, 1171, 1267
HpyCH4IV ACGT 6 cut(s) 126, 174, 440, 466, 527, 1132
HpyCH4V TGCA 8 cut(s) 57, 406, 470, 537, 542, 669, 740, 1009
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 282, 311, 348, 727
HpyF3I CTNAG 2 cut(s) 322, 726
HpySE526I ACGT 6 cut(s) 126, 174, 440, 466, 527, 1132
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 4 cut(s) 503, 869, 927, 1013
HspAI GCGC 1 cut(s) 152
KspAI GTTAAC 1 cut(s) 858
Kzo9I GATC 4 cut(s) 106, 223, 1251, 1327
LmnI GCTCC 4 cut(s) 311, 368, 518, 1021
Lsp1109I GCAGC 2 cut(s) 18, 158
LweI GCATC 2 cut(s) 360, 678
MabI ACCWGGT 1 cut(s) 946
MaeII ACGT 6 cut(s) 126, 174, 440, 466, 527, 1132
MalI GATC 4 cut(s) 108, 225, 1253, 1329
MbiI CCGCTC 1 cut(s) 725
MboI GATC 4 cut(s) 106, 223, 1251, 1327
MboII GAAGA 2 cut(s) 580, 1154
MflI RGATCY 2 cut(s) 223, 1327
MhlI GDGCHC 2 cut(s) 523, 723
MluCI AATT 9 cut(s) 367, 445, 593, 778, 810, 834, 969, 1066, 1207
MlyI GAGTC 3 cut(s) 176, 209, 260
MmeI TCCRAC 4 cut(s) 288, 378, 414, 1020
MseI TTAA 4 cut(s) 801, 857, 1155, 1272
MslI CAYNNNNRTG 2 cut(s) 735, 1265
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 4 cut(s) 40, 241, 452, 825
MspR9I CCNGG 5 cut(s) 32, 41, 71, 241, 948
MvaI CCWGG 3 cut(s) 32, 71, 948
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 5 cut(s) 58, 282, 311, 348, 727
NciI CCSGG 2 cut(s) 41, 241
NdeII GATC 4 cut(s) 106, 223, 1251, 1327
NlaIII CATG 4 cut(s) 503, 869, 927, 1013
NlaIV GGNNCC 4 cut(s) 22, 78, 245, 1023
NmeAIII GCCGAG 2 cut(s) 1074, 1206
NspI RCATGY 1 cut(s) 1013
NspV TTCGAA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 5 cut(s) 102, 117, 123, 132, 177
PfeI GAWTC 3 cut(s) 458, 821, 1127
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 4 cut(s) 8, 148, 151, 476
PleI GAGTC 3 cut(s) 176, 209, 260
PmaCI CACGTG 1 cut(s) 1133
PmlI CACGTG 1 cut(s) 1133
PpsI GAGTC 3 cut(s) 176, 209, 260
Ppu21I YACGTR 2 cut(s) 467, 1133
PpuMI RGGWCCY 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 629
PshBI ATTAAT 1 cut(s) 1155
PsiI TTATAA 2 cut(s) 389, 641
Psp124BI GAGCTC 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 3 cut(s) 30, 69, 946
PspCI CACGTG 1 cut(s) 1133
PspGI CCWGG 3 cut(s) 30, 69, 946
PspN4I GGNNCC 4 cut(s) 22, 78, 245, 1023
PspPI GGNCC 4 cut(s) 20, 76, 631, 1097
PspPPI RGGWCCY 1 cut(s) 631
PsuI RGATCY 2 cut(s) 223, 1327
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 735, 1265
SacI GAGCTC 1 cut(s) 523
SaqAI TTAA 4 cut(s) 801, 857, 1155, 1272
SatI GCNGC 4 cut(s) 7, 147, 150, 475
Sau3AI GATC 4 cut(s) 106, 223, 1251, 1327
Sau96I GGNCC 4 cut(s) 20, 76, 631, 1097
SchI GAGTC 3 cut(s) 176, 209, 260
ScrFI CCNGG 5 cut(s) 32, 41, 71, 241, 948
SduI GDGCHC 2 cut(s) 523, 723
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 2 cut(s) 360, 678
SfiI GGCCNNNNNGGCC 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 3 cut(s) 20, 76, 631
SmiMI CAYNNNNRTG 2 cut(s) 735, 1265
Sse9I AATT 9 cut(s) 367, 445, 593, 778, 810, 834, 969, 1066, 1207
SsiI CCGC 9 cut(s) 9, 102, 150, 163, 204, 235, 474, 611, 723
SspI AATATT 1 cut(s) 901
SstI GAGCTC 1 cut(s) 523
StyD4I CCNGG 5 cut(s) 30, 39, 69, 239, 946
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 4 cut(s) 557, 585, 1171, 1267
TaiI ACGT 6 cut(s) 129, 177, 443, 469, 530, 1135
TaqI TCGA 8 cut(s) 96, 132, 171, 372, 443, 672, 819, 1332
TasI AATT 9 cut(s) 367, 445, 593, 778, 810, 834, 969, 1066, 1207
TatI WGTACW 1 cut(s) 546
TauI GCSGC 2 cut(s) 152, 477
TfiI GAWTC 3 cut(s) 458, 821, 1127
Tru1I TTAA 4 cut(s) 801, 857, 1155, 1272
Tru9I TTAA 4 cut(s) 801, 857, 1155, 1272
TscAI CASTG 2 cut(s) 544, 1176
TseI GCWGC 2 cut(s) 6, 146
TspDTI ATGAA 3 cut(s) 478, 586, 827
TspRI CASTG 2 cut(s) 544, 1176
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 3 cut(s) 20, 76, 631
VspI ATTAAT 1 cut(s) 1155
XapI RAATTY 6 cut(s) 445, 593, 778, 834, 1066, 1207
XceI RCATGY 1 cut(s) 1013
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.