MD10G1296600.v1.1

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009422
Physical Location & Seq
Reverse (-)
38427161 .. 38431537
4377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1296600.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGAGCAGCGTTCAAGCTGGACCCCACGCCCTAGCCTTCCGGGTGATGAGGCTGTGCAAGCCCTCATTCCAGGTGGACCCCATTCCCCTCCTTCTCGACCCCGCCGATCTTGTCGTCGGCGAGGACATCTTCGACGACCCGATTGCCGCCGCCCAACTCCCTCGCCTCCTCGACTCCCCCGCCTCCTCGAACCCCTCCTCCTCCGCCTCCTCCGACTCCTCCGATCTCACCTACCGCACCCGATTCCTCCTCCACCACCCCTCCGACTCCATCGGCCTCTCCGGCCTCCTCGTCCTCCCCCAAGCCTTCGGAGCAATTTATTTAGGGGAGACATTTTGTAGCTATATTAGCATCAATAACAGCTCCAATTTCGAAGTCAGGGAAATTATAATCAAGGCGGAAATGCAAACAGAAAGGCAGAGGCTTTTGCTGCTGGACACGTCGAAATCTCCGGTTGAATCCATACGTGCAGGCGGCCGTTATGATTTCATTGTTGAACATGATGTCAAGGAACTCGGCGCTCACACGTTGGTATGCACTGCATTGTACTATGACGGGGAAGGAGAGCGTAAATATCTACCGCAGTTCTTCAAGTTCATTGTGGCCAATCCGCTTTCTGTCAGGACTAAGGTCCGTGTTGTAAAGGAAATCACGCTTTTAGAAGCTTGCATTGAAAATCATACCAAGTCAAATCTTCTTATGGACCAAGTTGAGTTTGAGCCCGCTCAGCATTGGAGTGCAAAAATCCTAAAAGCTGATGAACATCATTCTGAAAAGAATTCTCAAACAAGAGAGATATTTAAGCCACCAATTCTTATCAAATCTGGCGGAGGAATTCATAACTATCTTTATCAGTTAATATCACATGGTTCTGCCCAAGGGAAAGTTGAGGGAAGCAATATTCTTGGTAAACTTCAGATAACATGGCGAACTAATCTGGGCGAACCTGGTCGCCTACAGACACAGCAAATTATGGGAACTCCCATAACACGAAAGGATATTGAGTTGCATGTGGTGGAGGTGCCGTCTGCCATCAAGTTGGAAAGACCCTTTTCACTACATTTGAAATTAGAAAATGAGACAGATAAGGAACTCGGCCCCTTTGAAGTTTGGTTATCACAAGATGATTCACCTGAGGAGAAGGTTGTTGTGATCAATGGTCTTCAAACAGTGGTTTTGCCGAGGGTTGAGGCTTTTGGTTCCACAAATATCAATCTGAACCTCATTGCTACTAAACTTGGAGTTCAGAAGATCACGGGCATTACCGTGTTTAACATAAGAGAGAAAAAGTCATATGACCCTTTGCCAGATTTGGAGATTTTTGTGGATCTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF974

Protein Analysis

446

Amino Acids

49.6

Weight (kDa)

5.6

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC13_N PF06159 9 - 201 2.8e-36 Trafficking protein particle complex subunit 13, N-terminal
TRAPPC13_M PF23647 205 - 324 3.1e-20 Trafficking protein particle complex subunit 13, middle domain
TRAPPC13_C PF23643 342 - 433 1.8e-11 Trafficking protein particle complex subunit 13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 1021
AccBSI CCGCTC 1 cut(s) 725
AclWI GGATC 1 cut(s) 1335
AcoI YGGCCR 2 cut(s) 475, 603
AcsI RAATTY 2 cut(s) 778, 834
AcuI CTGAAG 1 cut(s) 899
AfaI GTAC 1 cut(s) 548
AflIII ACRYGT 2 cut(s) 438, 525
AgsI TTSAA 8 cut(s) 14, 458, 497, 592, 674, 1066, 1106, 1166
AjiI CACGTC 1 cut(s) 441
AjnI CCWGG 2 cut(s) 69, 946
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 5 cut(s) 17, 342, 363, 665, 755
AluI AGCT 5 cut(s) 17, 342, 363, 665, 755
Alw26I GTCTC 2 cut(s) 323, 1073
AlwI GGATC 1 cut(s) 1335
AoxI GGCC 5 cut(s) 274, 283, 475, 603, 1096
ApeKI GCWGC 2 cut(s) 6, 430
ApoI RAATTY 2 cut(s) 778, 834
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 5 cut(s) 20, 76, 631, 703, 1097
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 55, 220, 1122
AsuII TTCGAA 1 cut(s) 372
AvaII GGWCC 4 cut(s) 20, 76, 631, 703
AxyI CCTNAGG 1 cut(s) 1134
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 1021
BanII GRGCYC 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 1154
BbvI GCAGC 2 cut(s) 18, 417
BccI CCATC 2 cut(s) 278, 1040
BceAI ACGGC 2 cut(s) 462, 1009
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 2 cut(s) 71, 948
BclI TGATCA 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 323, 1073
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 956
BfoI RGCGCY 1 cut(s) 522
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 5 cut(s) 7, 147, 150, 431, 475
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 5 cut(s) 8, 148, 151, 432, 476
Bme1390I CCNGG 3 cut(s) 41, 71, 948
Bme18I GGWCC 4 cut(s) 20, 76, 631, 703
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 5 cut(s) 20, 76, 631, 703, 1097
BmiI GGNNCC 5 cut(s) 22, 78, 1023, 1099, 1201
BmrFI CCNGG 3 cut(s) 41, 71, 948
BmsI GCATC 1 cut(s) 360
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 1154
Bpu1102I GCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 372
BpuMI CCSGG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 467
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 2 cut(s) 877, 1181
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 245, 275, 1010, 1040
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 1 cut(s) 1224
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 2 cut(s) 71, 948
BseDI CCNNGG 2 cut(s) 877, 1181
BseJI GATNNNNATC 1 cut(s) 762
BseMI GCAATG 1 cut(s) 1224
BseMII CTCAG 2 cut(s) 740, 1125
BseRI GAGGAG 9 cut(s) 158, 175, 187, 190, 199, 208, 239, 278, 1151
BseX3I CGGCCG 1 cut(s) 475
BseXI GCAGC 2 cut(s) 18, 417
BsgI GTGCAG 1 cut(s) 489
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 5 cut(s) 276, 285, 477, 605, 1098
BshNI GGYRCC 1 cut(s) 1021
BsiEI CGRYCG 1 cut(s) 478
BsiSI CCGG 3 cut(s) 40, 282, 452
BsmAI GTCTC 2 cut(s) 323, 1073
BsnI GGCC 5 cut(s) 276, 285, 477, 605, 1098
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 5 cut(s) 106, 223, 1152, 1251, 1327
Bsp1720I GCTNAGC 1 cut(s) 726
BspANI GGCC 5 cut(s) 276, 285, 477, 605, 1098
BspCNI CTCAG 2 cut(s) 739, 1126
BspLI GGNNCC 5 cut(s) 22, 78, 1023, 1099, 1201
BspPI GGATC 1 cut(s) 1335
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 1021
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 1224
BssECI CCNNGG 2 cut(s) 877, 1181
BssMI GATC 5 cut(s) 106, 223, 1152, 1251, 1327
BssT1I CCWWGG 1 cut(s) 877
Bst2UI CCWGG 2 cut(s) 71, 948
Bst4CI ACNGT 2 cut(s) 1171, 1267
BstBAI YACGTR 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 59, 472, 667, 723
BstDEI CTNAG 3 cut(s) 627, 726, 1134
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 5 cut(s) 109, 226, 1155, 1254, 1330
BstMAI GTCTC 2 cut(s) 323, 1073
BstMBI GATC 5 cut(s) 106, 223, 1152, 1251, 1327
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 6 cut(s) 58, 282, 311, 348, 430, 727
BstNI CCWGG 2 cut(s) 71, 948
BstNSI RCATGY 1 cut(s) 1013
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 3 cut(s) 39, 69, 946
BstSFI CTRYAG 1 cut(s) 956
BstV1I GCAGC 2 cut(s) 18, 417
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 1 cut(s) 1327
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 1327
BstZI CGGCCG 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 276, 285, 477, 605, 1098
BtrI CACGTC 1 cut(s) 441
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 537, 1176
Cac8I GCNNGC 4 cut(s) 59, 472, 667, 723
CfoI GCGC 1 cut(s) 521
Cfr13I GGNCC 5 cut(s) 20, 76, 631, 703, 1097
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 1 cut(s) 547
CviAII CATG 4 cut(s) 500, 866, 924, 1010
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 3 cut(s) 627, 726, 1134
DpnI GATC 5 cut(s) 108, 225, 1154, 1253, 1329
DpnII GATC 5 cut(s) 106, 223, 1152, 1251, 1327
EaeI YGGCCR 2 cut(s) 475, 603
EagI CGGCCG 1 cut(s) 475
EciI GGCGGA 3 cut(s) 193, 413, 843
EclXI CGGCCG 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 877
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 4 cut(s) 20, 76, 631, 703
Eco52I CGGCCG 1 cut(s) 475
Eco57I CTGAAG 1 cut(s) 899
Eco81I CCTNAGG 1 cut(s) 1134
EcoRI GAATTC 2 cut(s) 778, 834
EcoRII CCWGG 2 cut(s) 69, 946
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 877
FaeI CATG 4 cut(s) 503, 869, 927, 1013
FatI CATG 4 cut(s) 499, 865, 923, 1009
FauI CCCGC 3 cut(s) 109, 187, 730
FauNDI CATATG 1 cut(s) 1294
FbaI TGATCA 1 cut(s) 1152
Fnu4HI GCNGC 5 cut(s) 7, 147, 150, 431, 475
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 5 cut(s) 7, 147, 150, 431, 475
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 520
GluI GCNGC 5 cut(s) 7, 147, 150, 431, 475
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 5 cut(s) 276, 285, 477, 605, 1098
HapII CCGG 3 cut(s) 40, 282, 452
HhaI GCGC 1 cut(s) 521
Hin1II CATG 4 cut(s) 503, 869, 927, 1013
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 6 cut(s) 173, 215, 243, 266, 458, 1127
HpaII CCGG 3 cut(s) 40, 282, 452
HphI GGTGA 3 cut(s) 55, 220, 1122
Hpy166II GTNNAC 2 cut(s) 76, 911
Hpy188I TCNGA 8 cut(s) 214, 223, 265, 311, 772, 918, 1218, 1248
Hpy188III TCNNGA 3 cut(s) 95, 622, 1331
Hpy8I GTNNAC 2 cut(s) 76, 911
Hpy99I CGWCG 3 cut(s) 119, 137, 445
HpyAV CCTTC 5 cut(s) 46, 101, 316, 554, 1135
HpyCH4III ACNGT 2 cut(s) 1171, 1267
HpyCH4IV ACGT 3 cut(s) 440, 466, 527
HpyCH4V TGCA 8 cut(s) 57, 406, 470, 537, 542, 669, 740, 1009
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 282, 311, 348, 430, 727
HpyF3I CTNAG 3 cut(s) 627, 726, 1134
HpySE526I ACGT 3 cut(s) 440, 466, 527
Hsp92II CATG 4 cut(s) 503, 869, 927, 1013
HspAI GCGC 1 cut(s) 519
Ksp22I TGATCA 1 cut(s) 1152
Kzo9I GATC 5 cut(s) 106, 223, 1152, 1251, 1327
LmnI GCTCC 2 cut(s) 311, 368
Lsp1109I GCAGC 2 cut(s) 18, 417
LweI GCATC 1 cut(s) 360
MabI ACCWGGT 1 cut(s) 946
MaeI CTAG 1 cut(s) 32
MaeII ACGT 3 cut(s) 440, 466, 527
MalI GATC 5 cut(s) 108, 225, 1154, 1253, 1329
MbiI CCGCTC 1 cut(s) 725
MboI GATC 5 cut(s) 106, 223, 1152, 1251, 1327
MboII GAAGA 5 cut(s) 121, 580, 686, 1154, 1261
MflI RGATCY 1 cut(s) 1327
MhlI GDGCHC 1 cut(s) 723
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 8 cut(s) 315, 367, 384, 778, 810, 834, 969, 1067
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 3 cut(s) 167, 209, 260
MmeI TCCRAC 3 cut(s) 237, 288, 1020
Mox20I TGGCCA 1 cut(s) 605
MscI TGGCCA 1 cut(s) 605
MseI TTAA 3 cut(s) 801, 857, 1272
MslI CAYNNNNRTG 2 cut(s) 735, 1265
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 3 cut(s) 40, 282, 452
MspR9I CCNGG 3 cut(s) 41, 71, 948
MvaI CCWGG 2 cut(s) 71, 948
MwoI GCNNNNNNNGC 6 cut(s) 58, 282, 311, 348, 430, 727
NciI CCSGG 1 cut(s) 41
NdeI CATATG 1 cut(s) 1294
NdeII GATC 5 cut(s) 106, 223, 1152, 1251, 1327
NlaIII CATG 4 cut(s) 503, 869, 927, 1013
NlaIV GGNNCC 5 cut(s) 22, 78, 1023, 1099, 1201
NmeAIII GCCGAG 3 cut(s) 495, 1074, 1206
NspI RCATGY 1 cut(s) 1013
NspV TTCGAA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 3 cut(s) 243, 458, 1127
PkrI GCNGC 5 cut(s) 8, 148, 151, 432, 476
PleI GAGTC 3 cut(s) 167, 209, 260
PpsI GAGTC 3 cut(s) 167, 209, 260
Ppu21I YACGTR 1 cut(s) 467
PshAI GACNNNNGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 69, 946
PspGI CCWGG 2 cut(s) 69, 946
PspN4I GGNNCC 5 cut(s) 22, 78, 1023, 1099, 1201
PspPI GGNCC 5 cut(s) 20, 76, 631, 703, 1097
PsuI RGATCY 1 cut(s) 1327
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 735, 1265
SaqAI TTAA 3 cut(s) 801, 857, 1272
SatI GCNGC 5 cut(s) 7, 147, 150, 431, 475
Sau3AI GATC 5 cut(s) 106, 223, 1152, 1251, 1327
Sau96I GGNCC 5 cut(s) 20, 76, 631, 703, 1097
SchI GAGTC 3 cut(s) 167, 209, 260
ScrFI CCNGG 3 cut(s) 41, 71, 948
SduI GDGCHC 1 cut(s) 723
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 1 cut(s) 360
SfcI CTRYAG 1 cut(s) 956
SfiI GGCCNNNNNGGCC 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 4 cut(s) 20, 76, 631, 703
SmiMI CAYNNNNRTG 2 cut(s) 735, 1265
Sse9I AATT 8 cut(s) 315, 367, 384, 778, 810, 834, 969, 1067
SspI AATATT 1 cut(s) 901
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 39, 69, 946
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 2 cut(s) 1171, 1267
TaiI ACGT 3 cut(s) 443, 469, 530
TaqI TCGA 7 cut(s) 96, 132, 171, 188, 372, 443, 1332
TasI AATT 8 cut(s) 315, 367, 384, 778, 810, 834, 969, 1067
TatI WGTACW 1 cut(s) 546
TauI GCSGC 3 cut(s) 149, 152, 477
TfiI GAWTC 3 cut(s) 243, 458, 1127
Tru1I TTAA 3 cut(s) 801, 857, 1272
Tru9I TTAA 3 cut(s) 801, 857, 1272
TscAI CASTG 2 cut(s) 544, 1176
TseI GCWGC 2 cut(s) 6, 430
TspDTI ATGAA 4 cut(s) 478, 586, 774, 827
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 544, 1176
VpaK11BI GGWCC 4 cut(s) 20, 76, 631, 703
XapI RAATTY 2 cut(s) 778, 834
XceI RCATGY 1 cut(s) 1013
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.