pycom10g24940

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009422
Physical Location & Seq
Reverse (-)
26663101 .. 26666768
3668 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24940.6

Sequence Viewer

Length: 1272 bp
ATGAGCAGCGGTCAAGCTGGACCCCACTCCCTAGCCTTCCGGGTGATGAGGCTGTGCAAGCCCTCGTTCCAGGTGGACCCCATTCCCCTCCTTCTGGACCCCGCCGATCTCGTCGTCGGCGAGGACATCTTCGACGACCCGATCGCCGCCGCTCAACTCCCCCGCCTCCTCGACTCCTCCGCCGCCTCGAACCCCTCCTCCGACTCCTCCGATCTCACCTACCGCACCCGTTTCCTCCTCCACCACCCCTCCGACTCCATCGGCCTCTCGGGCCTCCTCGTCCTCCCCCAAGCCTTCGGAGCAATTTATTTAGGGGAGACATTTTGTAGCTATATTAGCATCAATAACAGCTCCAATTTCGAAGTCAGGGACATTATAATCAAGGCGGAAATGCAAACAGAAAGGCAGAGGCTTTTGCTGCTGGACACGTCGAAATCTCCGGTTGAATCCATACGTGCAGGCGGCCGTTATGATTTCATTGTTGAACATGATGTCAAGGAACTCGGCGCTCACACGTTGGTATGCACTGCATTGTACTATGATGGGGAGGGAGAGCGTAAATATCTACCGCAGTTCTTCAAGTTCATTGTGGCCAATCCGCTTTCCGTCAGGACTAAGGTCCGTGTTGTAAAGGAAATCACGCTTTTAGAAGCTTGCATCGAGAATCATACCAAGTCAAATCTTCTTATGGACCAAGTTGAGTTTGAGCCCGCTCAGCATTGGAGTGCAAAAATCCTAAAAGCTGACGAACATCATTCTGAAAAGAATTCTCAAACAAGGTACAATATGTTAATATCACATGGTTCTGCCCAAGTGAAAGTTGAGGGAAGCAATATTCTTGGTAAACTTCAGATAACATGGCGAACTAATCTGGGCGAACCTGGTCGCCTGCAGACACAGCAAATTATGGGAACTCCCATAACACGAAAGGATATTGAGTTGCATGTGGTGGAGGTGCCGTCTGCCATCAAGTTGGAAAGACCCTTTTCGCTACATTTGAAATTAGAAAATGAGACAGATAAGGAACTCGGCCCCTTTGAAGTTTGGTTATCACAAGATGATTCACCTGAGGAGAAGGTTGTTGTGATCAATGGTCTTCAAACAGTGGTTTTGCCGAGGGTTGAGGCGTTTGGTTCCACAAATATCAATCTGAACCTCATTGCTACTAAACTTGGAGTTCAGAAGATCACGGGCATTACCGTGTTTAACACAAGAGAGAAAAAGTCATATGACCCTTTGCCAGATTTGGAGATTTTTGTGGATCTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF974

Protein Analysis

424

Amino Acids

47.27

Weight (kDa)

5.46

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 955
AccBSI CCGCTC 2 cut(s) 152, 713
AclWI GGATC 1 cut(s) 1269
AcoI YGGCCR 2 cut(s) 463, 591
AcsI RAATTY 1 cut(s) 766
AcuI CTGAAG 1 cut(s) 833
AfaI GTAC 2 cut(s) 536, 782
AfiI CCNNNNNNNGG 1 cut(s) 94
AflIII ACRYGT 2 cut(s) 426, 513
AgsI TTSAA 6 cut(s) 446, 485, 580, 1000, 1040, 1100
AjiI CACGTC 1 cut(s) 429
AjnI CCWGG 2 cut(s) 69, 880
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 5 cut(s) 17, 330, 351, 653, 743
AluI AGCT 5 cut(s) 17, 330, 351, 653, 743
Alw26I GTCTC 2 cut(s) 311, 1007
AlwI GGATC 1 cut(s) 1269
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 5 cut(s) 262, 271, 463, 591, 1030
ApeKI GCWGC 2 cut(s) 6, 418
ApoI RAATTY 1 cut(s) 766
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 7 cut(s) 20, 76, 97, 271, 619, 691, 1031
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 55, 208, 1056
AsuII TTCGAA 1 cut(s) 360
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 5 cut(s) 20, 76, 97, 619, 691
AxyI CCTNAGG 1 cut(s) 1068
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 1 cut(s) 955
BanII GRGCYC 1 cut(s) 711
BbsI GAAGAC 1 cut(s) 1088
BbvI GCAGC 2 cut(s) 18, 405
BccI CCATC 3 cut(s) 266, 536, 974
BceAI ACGGC 2 cut(s) 450, 943
BciT130I CCWGG 2 cut(s) 71, 882
BclI TGATCA 1 cut(s) 1086
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 311, 1007
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 890
BfoI RGCGCY 1 cut(s) 510
BglI GCCNNNNNGGC 1 cut(s) 270
BisI GCNGC 6 cut(s) 7, 147, 150, 183, 419, 463
BlpI GCTNAGC 1 cut(s) 714
BlsI GCNGC 6 cut(s) 8, 148, 151, 184, 420, 464
Bme1390I CCNGG 3 cut(s) 41, 71, 882
Bme18I GGWCC 5 cut(s) 20, 76, 97, 619, 691
BmeT110I CYCGRG 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 7 cut(s) 20, 76, 97, 271, 619, 691, 1031
BmiI GGNNCC 6 cut(s) 22, 78, 99, 957, 1033, 1135
BmrFI CCNGG 3 cut(s) 41, 71, 882
BmsI GCATC 2 cut(s) 348, 666
BoxI GACNNNNGTC 1 cut(s) 617
BpiI GAAGAC 1 cut(s) 1088
Bpu1102I GCTNAGC 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 1115
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 233, 263, 944, 974
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 1 cut(s) 1158
BseBI CCWGG 2 cut(s) 71, 882
BseDI CCNNGG 1 cut(s) 1115
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 1158
BseMII CTCAG 2 cut(s) 728, 1059
BseRI GAGGAG 7 cut(s) 158, 166, 187, 196, 227, 266, 1085
BseX3I CGGCCG 1 cut(s) 463
BseXI GCAGC 2 cut(s) 18, 405
BsgI GTGCAG 1 cut(s) 477
Bsh1285I CGRYCG 2 cut(s) 144, 466
BshFI GGCC 5 cut(s) 264, 273, 465, 593, 1032
BshNI GGYRCC 1 cut(s) 955
BsiEI CGRYCG 2 cut(s) 144, 466
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 2 cut(s) 40, 440
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 2 cut(s) 311, 1007
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 5 cut(s) 264, 273, 465, 593, 1032
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 711
Bsp143I GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
Bsp1720I GCTNAGC 1 cut(s) 714
BspANI GGCC 5 cut(s) 264, 273, 465, 593, 1032
BspCNI CTCAG 2 cut(s) 727, 1060
BspLI GGNNCC 6 cut(s) 22, 78, 99, 957, 1033, 1135
BspMAI CTGCAG 1 cut(s) 894
BspPI GGATC 1 cut(s) 1269
BspT104I TTCGAA 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 955
BsrBI CCGCTC 2 cut(s) 152, 713
BsrDI GCAATG 1 cut(s) 1158
BssECI CCNNGG 1 cut(s) 1115
BssMI GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
Bst2UI CCWGG 2 cut(s) 71, 882
Bst4CI ACNGT 2 cut(s) 1105, 1201
BstBAI YACGTR 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 360
BstC8I GCNNGC 5 cut(s) 59, 460, 655, 711, 890
BstDEI CTNAG 3 cut(s) 615, 714, 1068
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 6 cut(s) 109, 144, 214, 1089, 1188, 1264
BstMAI GTCTC 2 cut(s) 311, 1007
BstMBI GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
BstMCI CGRYCG 2 cut(s) 144, 466
BstMWI GCNNNNNNNGC 7 cut(s) 58, 270, 299, 336, 418, 715, 898
BstNI CCWGG 2 cut(s) 71, 882
BstNSI RCATGY 1 cut(s) 947
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 3 cut(s) 39, 69, 880
BstSFI CTRYAG 1 cut(s) 890
BstV1I GCAGC 2 cut(s) 18, 405
BstV2I GAAGAC 1 cut(s) 1088
BstX2I RGATCY 1 cut(s) 1261
BstXI CCANNNNNNTGG 1 cut(s) 973
BstYI RGATCY 1 cut(s) 1261
BstZI CGGCCG 1 cut(s) 463
Bsu36I CCTNAGG 1 cut(s) 1068
BsuRI GGCC 5 cut(s) 264, 273, 465, 593, 1032
BtrI CACGTC 1 cut(s) 429
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 525, 1110
Cac8I GCNNGC 5 cut(s) 59, 460, 655, 711, 890
CfoI GCGC 1 cut(s) 509
Cfr13I GGNCC 7 cut(s) 20, 76, 97, 271, 619, 691, 1031
CsiI ACCWGGT 1 cut(s) 880
Csp6I GTAC 2 cut(s) 535, 781
CviAII CATG 4 cut(s) 488, 800, 858, 944
CviQI GTAC 2 cut(s) 535, 781
DdeI CTNAG 3 cut(s) 615, 714, 1068
DpnI GATC 6 cut(s) 108, 143, 213, 1088, 1187, 1263
DpnII GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
EaeI YGGCCR 2 cut(s) 463, 591
EagI CGGCCG 1 cut(s) 463
EciI GGCGGA 2 cut(s) 169, 401
EclXI CGGCCG 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 711
Eco47I GGWCC 5 cut(s) 20, 76, 97, 619, 691
Eco52I CGGCCG 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 833
Eco81I CCTNAGG 1 cut(s) 1068
Eco88I CYCGRG 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 2 cut(s) 69, 880
EcoT38I GRGCYC 1 cut(s) 711
FaeI CATG 4 cut(s) 491, 803, 861, 947
FaqI GGGAC 1 cut(s) 383
FatI CATG 4 cut(s) 487, 799, 857, 943
FauI CCCGC 3 cut(s) 109, 170, 718
FauNDI CATATG 1 cut(s) 1228
FbaI TGATCA 1 cut(s) 1086
Fnu4HI GCNGC 6 cut(s) 7, 147, 150, 183, 419, 463
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 6 cut(s) 7, 147, 150, 183, 419, 463
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 508
GluI GCNGC 6 cut(s) 7, 147, 150, 183, 419, 463
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 5 cut(s) 264, 273, 465, 593, 1032
HapII CCGG 2 cut(s) 40, 440
HhaI GCGC 1 cut(s) 509
Hin1II CATG 4 cut(s) 491, 803, 861, 947
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HindIII AAGCTT 1 cut(s) 651
HinfI GANTC 6 cut(s) 173, 203, 254, 446, 664, 1061
HpaII CCGG 2 cut(s) 40, 440
HphI GGTGA 3 cut(s) 55, 208, 1056
Hpy166II GTNNAC 2 cut(s) 76, 845
Hpy188I TCNGA 8 cut(s) 202, 211, 253, 299, 760, 852, 1152, 1182
Hpy188III TCNNGA 4 cut(s) 95, 610, 661, 1265
Hpy8I GTNNAC 2 cut(s) 76, 845
Hpy99I CGWCG 4 cut(s) 116, 119, 137, 433
HpyAV CCTTC 4 cut(s) 46, 101, 304, 1069
HpyCH4III ACNGT 2 cut(s) 1105, 1201
HpyCH4IV ACGT 3 cut(s) 428, 454, 515
HpyCH4V TGCA 9 cut(s) 57, 394, 458, 525, 530, 657, 728, 892, 943
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 270, 299, 336, 418, 715, 898
HpyF3I CTNAG 3 cut(s) 615, 714, 1068
HpySE526I ACGT 3 cut(s) 428, 454, 515
Hsp92II CATG 4 cut(s) 491, 803, 861, 947
HspAI GCGC 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 1086
Kzo9I GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
LmnI GCTCC 2 cut(s) 299, 356
Lsp1109I GCAGC 2 cut(s) 18, 405
LweI GCATC 2 cut(s) 348, 666
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 1 cut(s) 32
MaeII ACGT 3 cut(s) 428, 454, 515
MalI GATC 6 cut(s) 108, 143, 213, 1088, 1187, 1263
MbiI CCGCTC 2 cut(s) 152, 713
MboI GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
MboII GAAGA 5 cut(s) 121, 568, 674, 1088, 1195
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 1 cut(s) 711
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 5 cut(s) 303, 355, 766, 903, 1001
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 3 cut(s) 167, 197, 248
MmeI TCCRAC 3 cut(s) 225, 276, 954
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MseI TTAA 2 cut(s) 791, 1206
MslI CAYNNNNRTG 2 cut(s) 723, 1199
Msp20I TGGCCA 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 2 cut(s) 40, 440
MspR9I CCNGG 3 cut(s) 41, 71, 882
MvaI CCWGG 2 cut(s) 71, 882
MwoI GCNNNNNNNGC 7 cut(s) 58, 270, 299, 336, 418, 715, 898
NciI CCSGG 1 cut(s) 41
NdeI CATATG 1 cut(s) 1228
NdeII GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
NlaIII CATG 4 cut(s) 491, 803, 861, 947
NlaIV GGNNCC 6 cut(s) 22, 78, 99, 957, 1033, 1135
NmeAIII GCCGAG 3 cut(s) 483, 1008, 1140
NspI RCATGY 1 cut(s) 947
NspV TTCGAA 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 3 cut(s) 446, 664, 1061
PkrI GCNGC 6 cut(s) 8, 148, 151, 184, 420, 464
Ple19I CGATCG 1 cut(s) 144
PleI GAGTC 3 cut(s) 167, 197, 248
PpsI GAGTC 3 cut(s) 167, 197, 248
Ppu21I YACGTR 1 cut(s) 455
PshAI GACNNNNGTC 1 cut(s) 617
PsiI TTATAA 1 cut(s) 377
Psp6I CCWGG 2 cut(s) 69, 880
PspGI CCWGG 2 cut(s) 69, 880
PspN4I GGNNCC 6 cut(s) 22, 78, 99, 957, 1033, 1135
PspPI GGNCC 7 cut(s) 20, 76, 97, 271, 619, 691, 1031
PstI CTGCAG 1 cut(s) 894
PsuI RGATCY 1 cut(s) 1261
PvuI CGATCG 1 cut(s) 144
RsaI GTAC 2 cut(s) 536, 782
RsaNI GTAC 2 cut(s) 535, 781
RseI CAYNNNNRTG 2 cut(s) 723, 1199
SaqAI TTAA 2 cut(s) 791, 1206
SatI GCNGC 6 cut(s) 7, 147, 150, 183, 419, 463
Sau3AI GATC 6 cut(s) 106, 141, 211, 1086, 1185, 1261
Sau96I GGNCC 7 cut(s) 20, 76, 97, 271, 619, 691, 1031
SchI GAGTC 3 cut(s) 167, 197, 248
ScrFI CCNGG 3 cut(s) 41, 71, 882
SduI GDGCHC 1 cut(s) 711
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 2 cut(s) 348, 666
SfcI CTRYAG 1 cut(s) 890
SfiI GGCCNNNNNGGCC 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 5 cut(s) 20, 76, 97, 619, 691
SmiMI CAYNNNNRTG 2 cut(s) 723, 1199
Sse9I AATT 5 cut(s) 303, 355, 766, 903, 1001
SspI AATATT 1 cut(s) 835
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 39, 69, 880
TaaI ACNGT 2 cut(s) 1105, 1201
TaiI ACGT 3 cut(s) 431, 457, 518
TaqI TCGA 7 cut(s) 132, 171, 188, 360, 431, 660, 1266
TasI AATT 5 cut(s) 303, 355, 766, 903, 1001
TatI WGTACW 1 cut(s) 534
TauI GCSGC 4 cut(s) 149, 152, 185, 465
TfiI GAWTC 3 cut(s) 446, 664, 1061
Tru1I TTAA 2 cut(s) 791, 1206
Tru9I TTAA 2 cut(s) 791, 1206
TscAI CASTG 2 cut(s) 532, 1110
TseI GCWGC 2 cut(s) 6, 418
TspDTI ATGAA 2 cut(s) 466, 574
TspGWI ACGGA 2 cut(s) 595, 611
TspRI CASTG 2 cut(s) 532, 1110
VpaK11BI GGWCC 5 cut(s) 20, 76, 97, 619, 691
XapI RAATTY 1 cut(s) 766
XceI RCATGY 1 cut(s) 947
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.