MD05G1351600.v1.1

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011270
Physical Location & Seq
Forward (+)
46853798 .. 46855500
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1351600.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGAATCCTCTGCGGCAATTCTCCCACTCTCTCGGCTCCCTTCTTTATTCCCACTAAAACCCAACAGAAGAAAATTACCTTACAATCGTTCTTGGCTATCCAATCCTAAACCAAGAGCCTCTCGTCCCCCTCGCATGCACTGCCACAAGATGTATGTCCCTGGATTTGGAGAACCCTCGCCGGAAGCAAAGGCGGCCAACAACCTCCACAGTTTCTTCACTTATGTTGCAGTTAGGATTGTCTGCGCTCAGCTTGAGCAGAGTTACAACACGGAGGCATACGAAGAGCTGATGGAATTTCTGAGCAGGAACTCGTTGAACGATGGAGACCAATTCTGTGCCAATCTGATGAGAGAATCCTCCAGGCATAAGGGTCTAGCTTTGCGCATCCTAGAGGTTCGATCTGCATACTGTAAAAATGATTTCGAGTGGGACAACTTGCAGCGATTAGCCTTCAAGATGGTGGATGAATCCAATACGAGGCTCATGAGAGATTATGTCCGAGAAATAAGTCATGTGGAAGGTGATCAGGAAAGTGAGAATTGA

Protein Analysis

182

Amino Acids

21.04

Weight (kDa)

8.35

Isoelectric Point (pI)

62.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 64 - 171 4.8e-29 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 386
AciI CCGC 2 cut(s) 14, 194
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 296
AfiI CCNNNNNNNGG 2 cut(s) 167, 480
AgsI TTSAA 2 cut(s) 319, 457
AjnI CCWGG 2 cut(s) 160, 362
AluBI AGCT 3 cut(s) 253, 289, 380
AluI AGCT 3 cut(s) 253, 289, 380
Alw26I GTCTC 1 cut(s) 321
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 2 cut(s) 248, 387
AsuHPI GGTGA 1 cut(s) 536
BbvI GCAGC 1 cut(s) 454
BccI CCATC 3 cut(s) 286, 317, 454
BciT130I CCWGG 2 cut(s) 162, 364
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 2 cut(s) 377, 392
BisI GCNGC 3 cut(s) 15, 195, 443
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 3 cut(s) 16, 196, 444
Bme1390I CCNGG 2 cut(s) 162, 364
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 2 cut(s) 162, 364
BmsI GCATC 1 cut(s) 396
BpmI CTGGAG 1 cut(s) 346
Bpu1102I GCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 480
BseBI CCWGG 2 cut(s) 162, 364
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 387, 472
BseLI CCNNNNNNNGG 2 cut(s) 167, 480
BseMII CTCAG 2 cut(s) 263, 293
BseXI GCAGC 1 cut(s) 454
BshFI GGCC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 3 cut(s) 111, 143, 446
BslI CCNNNNNNNGG 2 cut(s) 167, 480
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 3 cut(s) 111, 143, 446
BsnI GGCC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 401, 526
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 2 cut(s) 14, 194
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 262, 294
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 38
BspQI GCTCTTC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 321
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 401, 526
Bst2UI CCWGG 2 cut(s) 162, 364
Bst4CI ACNGT 2 cut(s) 212, 413
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 249, 302
BstF5I GGATG 2 cut(s) 387, 472
BstHHI GCGC 2 cut(s) 248, 387
BstKTI GATC 2 cut(s) 404, 529
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 401, 526
BstMWI GCNNNNNNNGC 2 cut(s) 141, 194
BstNI CCWGG 2 cut(s) 162, 364
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 160, 362
BstV1I GCAGC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 2 cut(s) 387, 472
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 137
CciI TCATGA 1 cut(s) 486
CfoI GCGC 2 cut(s) 248, 387
CviAII CATG 3 cut(s) 136, 487, 515
CviJI RGCY 9 cut(s) 37, 97, 119, 197, 253, 289, 380, 452, 484
CviKI_1 RGCY 9 cut(s) 37, 97, 119, 197, 253, 289, 380, 452, 484
DdeI CTNAG 2 cut(s) 249, 302
DpnI GATC 2 cut(s) 403, 528
DpnII GATC 2 cut(s) 401, 526
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 160, 362
FaeI CATG 3 cut(s) 139, 490, 518
FaiI YATR 9 cut(s) 137, 156, 225, 280, 369, 409, 488, 498, 516
FaqI GGGAC 3 cut(s) 111, 143, 446
FatI CATG 3 cut(s) 135, 486, 514
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 3 cut(s) 15, 195, 443
FokI GGATG 2 cut(s) 374, 479
Fsp4HI GCNGC 3 cut(s) 15, 195, 443
FspBI CTAG 2 cut(s) 377, 392
FspI TGCGCA 1 cut(s) 386
GlaI GCGC 2 cut(s) 247, 386
GluI GCNGC 3 cut(s) 15, 195, 443
GsuI CTGGAG 1 cut(s) 346
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 182
HhaI GCGC 2 cut(s) 248, 387
Hin1II CATG 3 cut(s) 139, 490, 518
Hin6I GCGC 2 cut(s) 246, 385
HinP1I GCGC 2 cut(s) 246, 385
HinfI GANTC 3 cut(s) 5, 356, 470
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 536
Hpy188I TCNGA 3 cut(s) 303, 348, 503
Hpy188III TCNNGA 3 cut(s) 457, 487, 530
HpyAV CCTTC 3 cut(s) 51, 463, 515
HpyCH4III ACNGT 2 cut(s) 212, 413
HpyCH4V TGCA 4 cut(s) 139, 230, 407, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 194
HpyF3I CTNAG 2 cut(s) 249, 302
Hsp92II CATG 3 cut(s) 139, 490, 518
HspAI GCGC 2 cut(s) 246, 385
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 2 cut(s) 401, 526
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 7 cut(s) 147, 174, 195, 292, 349, 376, 515
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 1 cut(s) 396
MaeI CTAG 2 cut(s) 377, 392
MaeIII GTNAC 1 cut(s) 263
MalI GATC 2 cut(s) 403, 528
MboI GATC 2 cut(s) 401, 526
MboII GAAGA 3 cut(s) 81, 208, 296
MluCI AATT 5 cut(s) 18, 74, 296, 332, 541
MnlI CCTC 9 cut(s) 19, 130, 141, 187, 215, 268, 370, 388, 474
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 162, 364
MvaI CCWGG 2 cut(s) 162, 364
MwoI GCNNNNNNNGC 2 cut(s) 141, 194
NdeII GATC 2 cut(s) 401, 526
NlaIII CATG 3 cut(s) 139, 490, 518
NlaIV GGNNCC 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 13
NsbI TGCGCA 1 cut(s) 386
NspI RCATGY 1 cut(s) 139
PaeI GCATGC 1 cut(s) 139
PagI TCATGA 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 5, 356, 470
PkrI GCNGC 3 cut(s) 16, 196, 444
Psp6I CCWGG 2 cut(s) 160, 362
PspGI CCWGG 2 cut(s) 160, 362
PspN4I GGNNCC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 279
SatI GCNGC 3 cut(s) 15, 195, 443
Sau3AI GATC 2 cut(s) 401, 526
ScrFI CCNGG 2 cut(s) 162, 364
SetI ASST 7 cut(s) 82, 207, 255, 291, 382, 399, 526
SfaNI GCATC 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SphI GCATGC 1 cut(s) 139
Sse9I AATT 5 cut(s) 18, 74, 296, 332, 541
SsiI CCGC 2 cut(s) 14, 194
SspMI CTAG 2 cut(s) 377, 392
StyD4I CCNGG 2 cut(s) 160, 362
TaaI ACNGT 2 cut(s) 212, 413
TaqI TCGA 2 cut(s) 400, 426
TasI AATT 5 cut(s) 18, 74, 296, 332, 541
TauI GCSGC 2 cut(s) 17, 197
TfiI GAWTC 3 cut(s) 5, 356, 470
TscAI CASTG 1 cut(s) 146
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 1 cut(s) 146
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 139
XspI CTAG 2 cut(s) 377, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.