Rh5CG020000

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011270
Physical Location & Seq
Reverse (-)
1298734 .. 1299780
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG020000.1

Sequence Viewer

Length: 384 bp
ATGTATGTCCCTGGATTTGGAGAAGCGTCCCCAGAAGCAAAGGCAGCGAATAACCTCCATAGTTTCTTCACTTATGTTGCGGTTAGCATTGTCACTGCACAGCTTCAGAGTTACAACACAGAGGCATATGAAGAGCTAATGGAGTTTTTGAGTCGAAACTCATTGAATGATGGAGACAAGTTCTGTGCCGATTTGATGCGAGAATCTTCCAGGCATCAGGCTCTAGCTTTGCGCATTTTAGAGGTTCGATCTGCATATTGTAAAAATGATTTTGAATGGGACAACCTAAAGCGACTGGCTTGCAAGATGGTGGATGAATCCAACACAAGACTCATGAGAGATTATGTCTTGGAAACAAGTCATGTGGACAATGAAGGAAAGTGA

Protein Analysis

127

Amino Acids

14.57

Weight (kDa)

5.03

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 14 - 118 7.1e-30 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 233
AciI CCGC 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 2 cut(s) 166, 275
AjnI CCWGG 2 cut(s) 10, 209
AluBI AGCT 3 cut(s) 103, 136, 227
AluI AGCT 3 cut(s) 103, 136, 227
Alw26I GTCTC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 164, 301
BcgI CGANNNNNNTGC 2 cut(s) 282, 316
BciT130I CCWGG 2 cut(s) 12, 211
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 224
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 12, 211
BmrFI CCNGG 2 cut(s) 12, 211
BmsI GCATC 2 cut(s) 186, 223
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 12, 211
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 17
BseNI ACTGG 1 cut(s) 300
BseXI GCAGC 1 cut(s) 56
BsgI GTGCAG 1 cut(s) 81
BslFI GGGAC 2 cut(s) 13, 293
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 2 cut(s) 13, 293
Bsp143I GATC 1 cut(s) 248
BspACI CCGC 1 cut(s) 80
BspHI TCATGA 1 cut(s) 333
BspQI GCTCTTC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 12, 211
Bst6I CTCTTC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 301
BstF5I GGATG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 1 cut(s) 251
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 248
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 2 cut(s) 12, 211
BstSCI CCNGG 2 cut(s) 10, 209
BstV1I GCAGC 1 cut(s) 56
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 301
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 234
CseI GACGC 1 cut(s) 15
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviAII CATG 2 cut(s) 334, 362
CviJI RGCY 5 cut(s) 103, 136, 221, 227, 299
CviKI_1 RGCY 5 cut(s) 103, 136, 221, 227, 299
DpnI GATC 1 cut(s) 250
DpnII GATC 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 10, 209
FaeI CATG 2 cut(s) 337, 365
FaiI YATR 9 cut(s) 6, 60, 75, 127, 129, 256, 335, 345, 363
FaqI GGGAC 2 cut(s) 13, 293
FatI CATG 2 cut(s) 333, 361
FauNDI CATATG 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 1 cut(s) 224
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 1 cut(s) 233
GluI GCNGC 1 cut(s) 45
HgaI GACGC 1 cut(s) 15
HhaI GCGC 1 cut(s) 234
Hin1II CATG 2 cut(s) 337, 365
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 4 cut(s) 151, 203, 317, 330
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 98, 254, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 337, 365
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 1 cut(s) 248
LguI GCTCTTC 1 cut(s) 126
LpnPI CCDG 6 cut(s) 24, 45, 196, 203, 223, 281
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 2 cut(s) 186, 223
MaeI CTAG 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 91, 110
MalI GATC 1 cut(s) 250
MboI GATC 1 cut(s) 248
MboII GAAGA 3 cut(s) 58, 143, 198
MlyI GAGTC 2 cut(s) 160, 324
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 3 cut(s) 65, 115, 235
MspR9I CCNGG 2 cut(s) 12, 211
MvaI CCWGG 2 cut(s) 12, 211
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeI CATATG 1 cut(s) 127
NdeII GATC 1 cut(s) 248
NlaIII CATG 2 cut(s) 337, 365
NmuCI GTSAC 1 cut(s) 91
NsbI TGCGCA 1 cut(s) 233
PagI TCATGA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 126
PfeI GAWTC 2 cut(s) 203, 317
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 2 cut(s) 159, 324
PpsI GAGTC 2 cut(s) 159, 324
Psp6I CCWGG 2 cut(s) 10, 209
PspGI CCWGG 2 cut(s) 10, 209
SapI GCTCTTC 1 cut(s) 126
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 1 cut(s) 248
SchI GAGTC 2 cut(s) 160, 324
ScrFI CCNGG 2 cut(s) 12, 211
SetI ASST 6 cut(s) 57, 105, 138, 229, 246, 288
SfaNI GCATC 2 cut(s) 186, 223
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 2 cut(s) 10, 209
TaqI TCGA 2 cut(s) 154, 247
TfiI GAWTC 2 cut(s) 203, 317
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 144, 330
TspRI CASTG 1 cut(s) 100
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.