RchiOBHm_Chr5g0002341

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011270
Physical Location & Seq
Reverse (-)
1355100 .. 1355951
852 bp
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UTR
Exon/CDS
Intron
PRQ28372

Sequence Viewer

Length: 567 bp
ATGGAATCCTCTGCAGCACTTGTCCCATTCTCTCAGCTCCCATCTTTCTTCTCACAAAACCCCAACAACAGAATTAGCAAAGCTCATCCTTCTTGGCCATCCAAACCAAGAACTTCTCTGCCAACTCGCATACACTGCCAAAAGATGTATGTCCCTGGATTTGGAGAAGCGTCCCCAGAAGCAAAGGCAGCGAATAACCTCCATAGTTTCTTCACTTATGTTGCGGTTAGCATTGTCACTGCACAGCTTGAGAGTTACAACAGAGAGGCATATGAAGAGCTAATGGAGTTTTTGAGTCGAAACTCATTGAATGATGGAGACAAGTTCTGTGCCGATTTGATGCGAGAATCTTCCAGGCATCAGGCTCTAGGTATGTTGATGTCTCAAATCTCTCAACCCTACAGAAAGTTCTATCATACTGATCAGTGTGGTATACATACTGGCTTTTGGTGGTTCTTTTCAATCGTTTTTCACTGTAAACTGAATCTATATGACACACTGATCAGTAGGTTGTACTTTAGGCATACTCAGCAAGCTAAGCTCAAAATTAAAAGGCTTCTAATCTGA

Protein Analysis

188

Amino Acids

21.81

Weight (kDa)

9.34

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 62 - 119 4.1e-16 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 433
AciI CCGC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 95
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 2 cut(s) 310, 462
AjnI CCWGG 2 cut(s) 154, 353
AluBI AGCT 6 cut(s) 37, 83, 247, 280, 536, 541
AluI AGCT 6 cut(s) 37, 83, 247, 280, 536, 541
Alw26I GTCTC 2 cut(s) 312, 387
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 2 cut(s) 14, 188
BalI TGGCCA 1 cut(s) 97
BbvI GCAGC 2 cut(s) 26, 200
BccI CCATC 3 cut(s) 49, 106, 308
BciT130I CCWGG 2 cut(s) 156, 355
BclI TGATCA 2 cut(s) 421, 501
BcoDI GTCTC 2 cut(s) 312, 387
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 2 cut(s) 12, 400
BisI GCNGC 2 cut(s) 15, 189
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 2 cut(s) 16, 190
Bme1390I CCNGG 2 cut(s) 156, 355
BmrFI CCNGG 2 cut(s) 156, 355
BmsI GCATC 2 cut(s) 330, 367
Bpu1102I GCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 2 cut(s) 156, 355
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 2 cut(s) 85, 98
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 2 cut(s) 47, 542
BseNI ACTGG 1 cut(s) 445
BseXI GCAGC 2 cut(s) 26, 200
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 1 cut(s) 97
BslFI GGGAC 3 cut(s) 8, 137, 157
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 312, 387
BsmFI GGGAC 3 cut(s) 8, 137, 157
BsnI GGCC 1 cut(s) 97
Bsp143I GATC 2 cut(s) 421, 501
Bsp1720I GCTNAGC 1 cut(s) 537
BspACI CCGC 1 cut(s) 224
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 2 cut(s) 46, 541
BspMAI CTGCAG 1 cut(s) 16
BspQI GCTCTTC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 2 cut(s) 421, 501
BssNAI GTATAC 1 cut(s) 434
Bst1107I GTATAC 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 156, 355
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 3 cut(s) 33, 528, 537
BstF5I GGATG 2 cut(s) 85, 98
BstKTI GATC 2 cut(s) 424, 504
BstMAI GTCTC 2 cut(s) 312, 387
BstMBI GATC 2 cut(s) 421, 501
BstMWI GCNNNNNNNGC 4 cut(s) 135, 188, 529, 538
BstNI CCWGG 2 cut(s) 156, 355
BstSCI CCNGG 2 cut(s) 154, 353
BstSFI CTRYAG 2 cut(s) 12, 400
BstV1I GCAGC 2 cut(s) 26, 200
BstZ17I GTATAC 1 cut(s) 434
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 2 cut(s) 85, 98
BtsI GCAGTG 2 cut(s) 133, 237
BtsIMutI CAGTG 5 cut(s) 133, 237, 431, 472, 497
Cac8I GCNNGC 1 cut(s) 534
CseI GACGC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 514
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 3 cut(s) 33, 528, 537
DpnI GATC 2 cut(s) 423, 503
DpnII GATC 2 cut(s) 421, 501
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EcoRII CCWGG 2 cut(s) 154, 353
FaqI GGGAC 3 cut(s) 8, 137, 157
FauNDI CATATG 1 cut(s) 271
FbaI TGATCA 2 cut(s) 421, 501
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 2 cut(s) 15, 189
FokI GGATG 2 cut(s) 72, 85
Fsp4HI GCNGC 2 cut(s) 15, 189
FspBI CTAG 1 cut(s) 368
GluI GCNGC 2 cut(s) 15, 189
HaeIII GGCC 1 cut(s) 97
HgaI GACGC 1 cut(s) 159
HinfI GANTC 4 cut(s) 5, 295, 347, 484
Hpy166II GTNNAC 2 cut(s) 434, 479
Hpy188I TCNGA 1 cut(s) 566
Hpy8I GTNNAC 2 cut(s) 434, 479
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4V TGCA 2 cut(s) 14, 242
HpyF10VI GCNNNNNNNGC 4 cut(s) 135, 188, 529, 538
HpyF3I CTNAG 3 cut(s) 33, 528, 537
Ksp22I TGATCA 2 cut(s) 421, 501
Kzo9I GATC 2 cut(s) 421, 501
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 7 cut(s) 141, 168, 189, 340, 347, 367, 426
Lsp1109I GCAGC 2 cut(s) 26, 200
LweI GCATC 2 cut(s) 330, 367
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 235, 254
MalI GATC 2 cut(s) 423, 503
MboI GATC 2 cut(s) 421, 501
MboII GAAGA 4 cut(s) 40, 202, 287, 342
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 2 cut(s) 72, 546
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 3 cut(s) 19, 209, 259
Mox20I TGGCCA 1 cut(s) 97
MscI TGGCCA 1 cut(s) 97
MseI TTAA 1 cut(s) 549
Msp20I TGGCCA 1 cut(s) 97
MspR9I CCNGG 2 cut(s) 156, 355
MvaI CCWGG 2 cut(s) 156, 355
MwoI GCNNNNNNNGC 4 cut(s) 135, 188, 529, 538
NdeI CATATG 1 cut(s) 271
NdeII GATC 2 cut(s) 421, 501
NmuCI GTSAC 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 5, 347, 484
PkrI GCNGC 2 cut(s) 16, 190
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 154, 353
PspGI CCWGG 2 cut(s) 154, 353
PstI CTGCAG 1 cut(s) 16
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 2 cut(s) 15, 189
Sau3AI GATC 2 cut(s) 421, 501
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 156, 355
SetI ASST 9 cut(s) 39, 85, 201, 249, 282, 373, 512, 538, 543
SfaNI GCATC 2 cut(s) 330, 367
SfcI CTRYAG 2 cut(s) 12, 400
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 72, 546
SsiI CCGC 1 cut(s) 224
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 154, 353
TaaI ACNGT 1 cut(s) 476
TaqI TCGA 1 cut(s) 298
TasI AATT 2 cut(s) 72, 546
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 3 cut(s) 5, 347, 484
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 5 cut(s) 140, 244, 431, 479, 504
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 2 cut(s) 14, 188
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 288
TspRI CASTG 5 cut(s) 140, 244, 431, 479, 504
XmiI GTMKAC 1 cut(s) 433
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.