MD10G1325900.v1.1

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011270
Physical Location & Seq
Forward (+)
40625349 .. 40627540
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1325900.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGAATCCTCTGCGGCAATACTCCCACTCTCTCAGCTCCCTTCTGTTTTCCCACAAAAACCCAACAGAAGCAATTTACCTTACAATCCTTCTTGGCTATCCAATTCCAAACCAAGAGCCTCTCGTCCCCCTCGCATGCACTGCCACAAGATGTATGTCCCTGGATTTGGAGAAGCGTCGCCGGAAGCAAAGGCGGCCAACAACCTCCACAGTTTCTTCACTTATGTTGCAGTTAGCATTGTCTGTGCTCAGCTTGAGCAGAGTTACAACACGGAGGCATACGAAGAGCTGATGGAATTTCTGAGTAGGAACTCGTTGAATGATGGAGACCAATTCTGTGCCAATCTGATGAGAGAATCTTCCAGGCATAAGGGTCTAGCTTTGCGCATCCTAGAGGTTCGATCTGCATACTGCAAAAATGATTTCGAGTGGGACAACCTGCAGCGATTAGCCTTCAAGATGGTGGATGAATCGAATACGAGGCTCATGAGAGATTATGTCCTAGAAATAAGTCATGTGGAAGGTGAACAGGGGAAGTGA

Protein Analysis

180

Amino Acids

20.47

Weight (kDa)

6.52

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 64 - 170 9.9e-28 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 447
AciI CCGC 2 cut(s) 14, 194
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 319, 457
AjnI CCWGG 2 cut(s) 160, 362
AluBI AGCT 4 cut(s) 37, 253, 289, 380
AluI AGCT 4 cut(s) 37, 253, 289, 380
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 321
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 1 cut(s) 387
AsuHPI GGTGA 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 454
BccI CCATC 3 cut(s) 286, 317, 454
BciT130I CCWGG 2 cut(s) 162, 364
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 3 cut(s) 377, 392, 503
BfmI CTRYAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 447
BisI GCNGC 3 cut(s) 15, 195, 443
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 3 cut(s) 16, 196, 444
Bme1390I CCNGG 2 cut(s) 162, 364
BmrFI CCNGG 2 cut(s) 162, 364
BmsI GCATC 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 167
BseBI CCWGG 2 cut(s) 162, 364
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 387, 472
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 3 cut(s) 47, 263, 293
BseXI GCAGC 1 cut(s) 454
BshFI GGCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 3 cut(s) 111, 143, 446
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 3 cut(s) 111, 143, 446
BsnI GGCC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 1 cut(s) 401
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 2 cut(s) 14, 194
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 3 cut(s) 46, 262, 294
BspHI TCATGA 1 cut(s) 486
BspMAI CTGCAG 1 cut(s) 444
BspMI ACCTGC 1 cut(s) 447
BspQI GCTCTTC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 321
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 1 cut(s) 401
Bst2UI CCWGG 2 cut(s) 162, 364
Bst4CI ACNGT 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 3 cut(s) 33, 249, 302
BstF5I GGATG 2 cut(s) 387, 472
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 1 cut(s) 404
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 1 cut(s) 401
BstMWI GCNNNNNNNGC 2 cut(s) 141, 194
BstNI CCWGG 2 cut(s) 162, 364
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 160, 362
BstSFI CTRYAG 1 cut(s) 440
BstV1I GCAGC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 2 cut(s) 387, 472
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 137
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 387
CseI GACGC 1 cut(s) 165
CviAII CATG 3 cut(s) 136, 487, 515
CviJI RGCY 9 cut(s) 37, 97, 119, 197, 253, 289, 380, 452, 484
CviKI_1 RGCY 9 cut(s) 37, 97, 119, 197, 253, 289, 380, 452, 484
DdeI CTNAG 3 cut(s) 33, 249, 302
DpnI GATC 1 cut(s) 403
DpnII GATC 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 160, 362
FaeI CATG 3 cut(s) 139, 490, 518
FaiI YATR 9 cut(s) 137, 156, 225, 280, 369, 409, 488, 498, 516
FaqI GGGAC 3 cut(s) 111, 143, 446
FatI CATG 3 cut(s) 135, 486, 514
Fnu4HI GCNGC 3 cut(s) 15, 195, 443
FokI GGATG 2 cut(s) 374, 479
Fsp4HI GCNGC 3 cut(s) 15, 195, 443
FspBI CTAG 3 cut(s) 377, 392, 503
FspI TGCGCA 1 cut(s) 386
GlaI GCGC 1 cut(s) 386
GluI GCNGC 3 cut(s) 15, 195, 443
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 182
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 387
Hin1II CATG 3 cut(s) 139, 490, 518
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HinfI GANTC 3 cut(s) 5, 356, 470
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 2 cut(s) 303, 348
Hpy188III TCNNGA 2 cut(s) 457, 487
Hpy8I GTNNAC 1 cut(s) 527
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 51, 99, 463, 515
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4V TGCA 5 cut(s) 139, 230, 407, 414, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 194
HpyF3I CTNAG 3 cut(s) 33, 249, 302
Hsp92II CATG 3 cut(s) 139, 490, 518
HspAI GCGC 1 cut(s) 385
Kzo9I GATC 1 cut(s) 401
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 7 cut(s) 147, 174, 195, 349, 376, 452, 515
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 1 cut(s) 396
MaeI CTAG 3 cut(s) 377, 392, 503
MaeIII GTNAC 1 cut(s) 263
MalI GATC 1 cut(s) 403
MboI GATC 1 cut(s) 401
MboII GAAGA 3 cut(s) 208, 296, 351
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 4 cut(s) 73, 103, 296, 332
MnlI CCTC 7 cut(s) 19, 130, 141, 215, 268, 388, 474
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 162, 364
MvaI CCWGG 2 cut(s) 162, 364
MwoI GCNNNNNNNGC 2 cut(s) 141, 194
NdeII GATC 1 cut(s) 401
NlaIII CATG 3 cut(s) 139, 490, 518
NsbI TGCGCA 1 cut(s) 386
NspI RCATGY 1 cut(s) 139
PaeI GCATGC 1 cut(s) 139
PagI TCATGA 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 5, 356, 470
PkrI GCNGC 3 cut(s) 16, 196, 444
Psp6I CCWGG 2 cut(s) 160, 362
PspGI CCWGG 2 cut(s) 160, 362
PstI CTGCAG 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 279
SatI GCNGC 3 cut(s) 15, 195, 443
Sau3AI GATC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 162, 364
SduI GDGCHC 1 cut(s) 250
SetI ASST 9 cut(s) 39, 82, 207, 255, 291, 382, 399, 441, 526
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SphI GCATGC 1 cut(s) 139
Sse9I AATT 4 cut(s) 73, 103, 296, 332
SsiI CCGC 2 cut(s) 14, 194
SspMI CTAG 3 cut(s) 377, 392, 503
StyD4I CCNGG 2 cut(s) 160, 362
TaaI ACNGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 400, 426, 473
TasI AATT 4 cut(s) 73, 103, 296, 332
TauI GCSGC 2 cut(s) 17, 197
TfiI GAWTC 3 cut(s) 5, 356, 470
TscAI CASTG 1 cut(s) 146
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 1 cut(s) 146
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 139
XspI CTAG 3 cut(s) 377, 392, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.