MD05G1352100.v1.1

DUF246 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002069
Physical Location & Seq
Forward (+)
46879029 .. 46879569
541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1352100.v1.1.491

Sequence Viewer

Length: 414 bp
ATGATTGCTTTGACTAGCTTGTATCCGGAATTAGTGACTAAAGAAAATTTGCTCACACCGACTGAACTCGAACCTTACATGAATTTTTCATCACAGTTAGCAGCACTGGACTTCATAGGCTGCACTGCTGCCGATGCATTTGCCATGACCGATTCAGGAAGTCAGTTGTCATCCTTGGTATCCGGTTTTCGAATATACTATGGTGGAGGAAAAATGCCAACAATACGGCCAAACAAACGCAGGCTTTCAAGCATCTTTATGAACAACTCCACTATTGAATGGCGTGTGTTCGAGCAGAGAATTCGAAAAGCAGTTAGACAAACTAAACACATCCAGCCAAGGCCGAAGGCAAGGAGTATGTATAGGTATCCACGGTGTGACGAGTGTATGTGCAACATGACAATATCCTCCTGA

Protein Analysis

138

Amino Acids

15.65

Weight (kDa)

9.51

Isoelectric Point (pI)

64.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 67 2.5e-11 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 227
AcsI RAATTY 3 cut(s) 46, 82, 300
AdeI CACNNNGTG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 249, 278
AluBI AGCT 1 cut(s) 18
AluI AGCT 1 cut(s) 18
Aor13HI TCCGGA 1 cut(s) 25
AoxI GGCC 2 cut(s) 227, 341
ApeKI GCWGC 3 cut(s) 101, 120, 128
ApoI RAATTY 3 cut(s) 46, 82, 300
AsuII TTCGAA 2 cut(s) 190, 304
BbvI GCAGC 3 cut(s) 107, 113, 115
BceAI ACGGC 1 cut(s) 242
BcgI CGANNNNNNTGC 4 cut(s) 122, 156, 284, 318
BciVI GTATCC 3 cut(s) 33, 190, 378
BfaI CTAG 1 cut(s) 15
BfuI GTATCC 3 cut(s) 33, 190, 378
BisI GCNGC 3 cut(s) 102, 121, 129
BlsI GCNGC 3 cut(s) 103, 122, 130
BmsI GCATC 2 cut(s) 124, 261
Bpu14I TTCGAA 2 cut(s) 190, 304
BsaJI CCNNGG 3 cut(s) 174, 338, 371
BsaWI WCCGGW 2 cut(s) 25, 182
Bse1I ACTGG 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 25
BseDI CCNNGG 3 cut(s) 174, 338, 371
BseGI GGATG 2 cut(s) 170, 330
BseNI ACTGG 1 cut(s) 111
BseXI GCAGC 3 cut(s) 107, 113, 115
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 2 cut(s) 229, 343
BsiSI CCGG 2 cut(s) 26, 183
BsnI GGCC 2 cut(s) 229, 343
Bsp119I TTCGAA 2 cut(s) 190, 304
Bsp13I TCCGGA 1 cut(s) 25
BspANI GGCC 2 cut(s) 229, 343
BspEI TCCGGA 1 cut(s) 25
BspT104I TTCGAA 2 cut(s) 190, 304
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 3 cut(s) 174, 338, 371
BssT1I CCWWGG 2 cut(s) 174, 338
Bst4CI ACNGT 2 cut(s) 96, 375
BstBI TTCGAA 2 cut(s) 190, 304
BstC8I GCNNGC 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 170, 330
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstV1I GCAGC 3 cut(s) 107, 113, 115
BsuI GTATCC 3 cut(s) 33, 190, 378
BsuRI GGCC 2 cut(s) 229, 343
BtgI CCRYGG 1 cut(s) 371
BtsCI GGATG 2 cut(s) 170, 330
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 104, 123
Cac8I GCNNGC 1 cut(s) 242
CviAII CATG 3 cut(s) 79, 145, 397
CviJI RGCY 6 cut(s) 18, 120, 229, 244, 337, 343
CviKI_1 RGCY 6 cut(s) 18, 120, 229, 244, 337, 343
DraIII CACNNNGTG 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 174, 338
EcoRI GAATTC 1 cut(s) 300
EcoT14I CCWWGG 2 cut(s) 174, 338
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 174, 338
FaeI CATG 3 cut(s) 82, 148, 400
FatI CATG 3 cut(s) 78, 144, 396
Fnu4HI GCNGC 3 cut(s) 102, 121, 129
FokI GGATG 2 cut(s) 157, 317
Fsp4HI GCNGC 3 cut(s) 102, 121, 129
FspBI CTAG 1 cut(s) 15
GluI GCNGC 3 cut(s) 102, 121, 129
HaeIII GGCC 2 cut(s) 229, 343
HapII CCGG 2 cut(s) 26, 183
Hin1II CATG 3 cut(s) 82, 148, 400
HinfI GANTC 1 cut(s) 152
HpaII CCGG 2 cut(s) 26, 183
Hpy188III TCNNGA 3 cut(s) 26, 156, 411
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 96, 375
HpyCH4V TGCA 3 cut(s) 123, 137, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
Hsp92II CATG 3 cut(s) 82, 148, 400
Kpn2I TCCGGA 1 cut(s) 25
LpnPI CCDG 6 cut(s) 39, 92, 141, 196, 226, 347
Lsp1109I GCAGC 3 cut(s) 107, 113, 115
LweI GCATC 2 cut(s) 124, 261
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 34, 377
MluCI AATT 4 cut(s) 29, 46, 82, 300
MnlI CCTC 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 139
MroI TCCGGA 1 cut(s) 25
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 2 cut(s) 26, 183
MwoI GCNNNNNNNGC 1 cut(s) 134
NlaIII CATG 3 cut(s) 82, 148, 400
NmuCI GTSAC 2 cut(s) 34, 377
NsiI ATGCAT 1 cut(s) 139
NspV TTCGAA 2 cut(s) 190, 304
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 103, 122, 130
RseI CAYNNNNRTG 1 cut(s) 257
SatI GCNGC 3 cut(s) 102, 121, 129
SetI ASST 3 cut(s) 20, 76, 368
SfaNI GCATC 2 cut(s) 124, 261
SfuI TTCGAA 2 cut(s) 190, 304
SmiMI CAYNNNNRTG 1 cut(s) 257
Sse9I AATT 4 cut(s) 29, 46, 82, 300
SspMI CTAG 1 cut(s) 15
StyI CCWWGG 2 cut(s) 174, 338
TaaI ACNGT 2 cut(s) 96, 375
TaqI TCGA 4 cut(s) 69, 190, 291, 304
TaqII GACCGA 1 cut(s) 164
TasI AATT 4 cut(s) 29, 46, 82, 300
TfiI GAWTC 1 cut(s) 152
TscAI CASTG 2 cut(s) 111, 130
TseFI GTSAC 2 cut(s) 34, 377
TseI GCWGC 3 cut(s) 101, 120, 128
Tsp45I GTSAC 2 cut(s) 34, 377
TspDTI ATGAA 4 cut(s) 78, 95, 103, 275
TspRI CASTG 2 cut(s) 111, 130
XapI RAATTY 3 cut(s) 46, 82, 300
XspI CTAG 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.