Rroxscaffold_1G00073660

DUF246 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002069
Physical Location & Seq
Forward (+)
94407539 .. 94411490
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073660.1

Sequence Viewer

Length: 1665 bp
ATGTCCCTCACCGCCGTCCAAGACACCCCTGAATCCGAATCAGAAGACGGGCCCAATTCAGATCAGGGCGAGCCCAATGCCGCCTGGCCCAGCAGCTCGTTTCATTTCCGGTCGGGGACTCCGAGGAGCCGCCACTCCTCTCCGGGTCACTCGCCCCGACCCGGCACCGGCACCGGGAGCGGCCACACTAGGTATGATGGCTACTTTGCGATTTGGATAGAGAATGTGATTAAGTATATGAAAGAGATTCTGATTGAGATGAAAGAGAGAGAGGGTATCTATGTGGGAGGAAGGAGTGAGGGAGTTGGGGAAAAGTTGAAAAAATATGGTAAAGGGAAGAAGCCAGTGAAAGGTTACAAAAGAATGTTGGCTTTAGCTGCTCATGCCTTGGCAGAGGGACATAAGGGAAAGAATGGATATATAATGGTCACTGCAAATGGTGGGATGAACCAGCAGCGGGTTGCGGTTTGCAACATTGTTGTTATTGCACGATTACTCAATGCAACCCTGGTGGTCCCCAAATTTTTGTATAGTAGTATATGGAGGGATGTAAGTCAATTCAGCGATATCTATCAGGAGGATCATTTTATTAACTACTTGAGTCCTGATATTCGGATAGTGAAGGAACTTCCTAAGGAACTACAGTCATTAAATTTGGAAGCTATTGGTAGCCTTGTGACAGATGCGGACATCCCTAAAGAGGCAAAGCCAAGTTTTTACTTGAAAAACATTACCCCTATTCTACTTAAAAATGGGGTTGTACATTTTCTTGGATTTGGGAATCGATTGGCATTTGATCCAATACCATTTCAGTTGCAGATACAAAAAATGGGTGCATTACTTCTTCAAAGGTTGCGTCACAGTGAAAGTCATGCTGGACCACTGGATCATTATCTGGTTGGTCCATATGCAGCACCAATCAAGAAAGAAAATGAAGGTCCTGCTAAGAAAGCTTCCAAGTATCTGGCTTTACACCTGAGGTTTGAAATTGACATGGTAGCTCATTCTTTGTGTGAATTTGGTGGAGGAGATGCGGAGCAAAAGGAGTTGGAAGCTTTTCGACAAATCCATTTCCCTGCATTGGTGCTTCTCCAGAATACTACAAAGCTACCTACTCCTGCAGTACTCAGGTCAGAAGGCTTGTGTCCTTTGTCGCCTGAAGAAGCAGTGCTTATGCTTGCTGCTCTGGGTTTCAAGCGTAAGACACATATATTTGTGGCAGGTTCACAAATATATGGAGGGAGATCAAGGATGATTGCTTTGACGAGCTTGTACCCTAAATTAGTGACTAAAGAAAACTTGCTTACACCTGCTGAACTTGAACCTTTCAAGAATTTTAAATCTCAGTTAGCAGCACTGGACTTCATAGGCTGCACTGCGGCCGATGCATTTGCCATGACTGATTCTGGAAGTCAATTATCATCCCTGGTAACTGGTTTTCGCATATACTATGGTGGAGGGATAACACCAACAATACGACCAAACAAACGGAGGCTTGCTAGCATTTTTTCAAAGAACTCCAGTATTGAATGGCGTGTGTTTGAGCAGAGAGTGAGGAAGGCGGTTAGACAAACCAAACATGTTCAGCCAAGACCCAGGGCAAGGAGTGTTTATCGGTATCCGCGGTGTGACGAGTGTATGTGCAATATGACATTAGTGATATAA

Protein Analysis

554

Amino Acids

61.63

Weight (kDa)

9.49

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 140 - 484 4e-83 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1318
Acc36I ACCTGC 2 cut(s) 1211, 1318
AccB1I GGYRCC 2 cut(s) 164, 170
AccBSI CCGCTC 1 cut(s) 180
AccII CGCG 1 cut(s) 1624
AclWI GGATC 3 cut(s) 588, 791, 894
AcoI YGGCCR 2 cut(s) 181, 1380
AcsI RAATTY 4 cut(s) 521, 652, 1016, 1333
AcuI CTGAAG 1 cut(s) 1179
AfaI GTAC 3 cut(s) 762, 1125, 1274
AfiI CCNNNNNNNGG 8 cut(s) 161, 167, 174, 350, 457, 700, 1081, 1602
AflIII ACRYGT 1 cut(s) 1579
AgsI TTSAA 9 cut(s) 319, 724, 848, 986, 1195, 1322, 1330, 1512, 1529
AjnI CCWGG 4 cut(s) 83, 507, 1425, 1595
AluBI AGCT 8 cut(s) 96, 377, 662, 953, 1001, 1055, 1108, 1269
AluI AGCT 8 cut(s) 96, 377, 662, 953, 1001, 1055, 1108, 1269
AlwI GGATC 3 cut(s) 588, 791, 894
AoxI GGCC 4 cut(s) 50, 86, 181, 1380
ApaI GGGCCC 1 cut(s) 54
ApeKI GCWGC 7 cut(s) 93, 377, 454, 911, 1181, 1352, 1371
ApoI RAATTY 4 cut(s) 521, 652, 1016, 1333
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
Asp700I GAANNNNTTC 1 cut(s) 1056
AspS9I GGNCC 7 cut(s) 50, 51, 87, 514, 878, 902, 938
AsuC2I CCSGG 3 cut(s) 144, 162, 175
AsuNHI GCTAGC 1 cut(s) 1499
AvaII GGWCC 4 cut(s) 514, 878, 902, 938
AxyI CCTNAGG 2 cut(s) 633, 977
BaeGI GKGCMC 1 cut(s) 54
BanI GGYRCC 2 cut(s) 164, 170
BanII GRGCYC 2 cut(s) 54, 75
BbsI GAAGAC 1 cut(s) 51
BbvI GCAGC 7 cut(s) 105, 364, 466, 923, 1168, 1358, 1364
BccI CCATC 1 cut(s) 191
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 1373, 1407
BciT130I CCWGG 4 cut(s) 85, 509, 1427, 1597
BciVI GTATCC 1 cut(s) 1629
BcnI CCSGG 3 cut(s) 144, 162, 175
BfaI CTAG 2 cut(s) 189, 1500
BfmI CTRYAG 2 cut(s) 641, 1119
BfuAI ACCTGC 2 cut(s) 1211, 1318
BfuI GTATCC 1 cut(s) 1629
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 7 cut(s) 85, 144, 162, 175, 509, 1427, 1597
Bme18I GGWCC 4 cut(s) 514, 878, 902, 938
BmgT120I GGNCC 7 cut(s) 50, 51, 87, 514, 878, 902, 938
BmiI GGNNCC 5 cut(s) 52, 128, 166, 172, 516
BmrFI CCNGG 7 cut(s) 85, 144, 162, 175, 509, 1427, 1597
BmsI GCATC 3 cut(s) 673, 1021, 1375
BmtI GCTAGC 1 cut(s) 1503
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 1076, 1504
BpuEI CTTGAG 1 cut(s) 619
BpuMI CCSGG 3 cut(s) 144, 162, 175
Bsa29I ATCGAT 1 cut(s) 784
BsaBI GATNNNNATC 2 cut(s) 570, 891
BsaJI CCNNGG 7 cut(s) 122, 387, 507, 1425, 1595, 1596, 1622
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 167, 174, 350, 457, 700, 1081, 1602
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 5 cut(s) 344, 888, 1362, 1438, 1521
Bse21I CCTNAGG 2 cut(s) 633, 977
Bse8I GATNNNNATC 2 cut(s) 570, 891
BseBI CCWGG 4 cut(s) 85, 509, 1427, 1597
BseCI ATCGAT 1 cut(s) 784
BseDI CCNNGG 7 cut(s) 122, 387, 507, 1425, 1595, 1596, 1622
BseGI GGATG 5 cut(s) 450, 553, 690, 1257, 1421
BseJI GATNNNNATC 2 cut(s) 570, 891
BseLI CCNNNNNNNGG 8 cut(s) 161, 167, 174, 350, 457, 700, 1081, 1602
BseMII CTCAG 3 cut(s) 968, 1141, 1358
BseNI ACTGG 5 cut(s) 344, 888, 1362, 1438, 1521
BseRI GAGGAG 3 cut(s) 127, 139, 1041
BseSI GKGCMC 1 cut(s) 54
BseX3I CGGCCG 1 cut(s) 1380
BseXI GCAGC 7 cut(s) 105, 364, 466, 923, 1168, 1358, 1364
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 1357
Bsh1236I CGCG 1 cut(s) 1624
Bsh1285I CGRYCG 2 cut(s) 113, 1383
BshFI GGCC 4 cut(s) 52, 88, 183, 1382
BshNI GGYRCC 2 cut(s) 164, 170
BshVI ATCGAT 1 cut(s) 784
BsiEI CGRYCG 2 cut(s) 113, 1383
BsiSI CCGG 5 cut(s) 109, 143, 162, 168, 174
BslFI GGGAC 3 cut(s) 130, 411, 500
BslI CCNNNNNNNGG 8 cut(s) 161, 167, 174, 350, 457, 700, 1081, 1602
BsmFI GGGAC 3 cut(s) 130, 411, 500
BsnI GGCC 4 cut(s) 52, 88, 183, 1382
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 54, 75
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 5 cut(s) 61, 580, 796, 886, 1244
BspANI GGCC 4 cut(s) 52, 88, 183, 1382
BspCNI CTCAG 3 cut(s) 969, 1140, 1357
BspDI ATCGAT 1 cut(s) 784
BspFNI CGCG 1 cut(s) 1624
BspLI GGNNCC 5 cut(s) 52, 128, 166, 172, 516
BspMAI CTGCAG 1 cut(s) 1123
BspMI ACCTGC 2 cut(s) 1211, 1318
BspOI GCTAGC 1 cut(s) 1503
BspPI GGATC 3 cut(s) 588, 791, 894
BspT107I GGYRCC 2 cut(s) 164, 170
BsrBI CCGCTC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 5 cut(s) 344, 888, 1362, 1438, 1521
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 7 cut(s) 122, 387, 507, 1425, 1595, 1596, 1622
BssMI GATC 5 cut(s) 61, 580, 796, 886, 1244
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 4 cut(s) 85, 509, 1427, 1597
Bst4CI ACNGT 2 cut(s) 645, 863
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 4 cut(s) 71, 1179, 1497, 1501
BstDEI CTNAG 5 cut(s) 633, 945, 977, 1127, 1344
BstDSI CCRYGG 1 cut(s) 1622
BstF5I GGATG 5 cut(s) 450, 553, 690, 1257, 1421
BstFNI CGCG 1 cut(s) 1624
BstKTI GATC 5 cut(s) 64, 583, 799, 889, 1247
BstMBI GATC 5 cut(s) 61, 580, 796, 886, 1244
BstMCI CGRYCG 2 cut(s) 113, 1383
BstMWI GCNNNNNNNGC 5 cut(s) 177, 377, 383, 950, 1385
BstNI CCWGG 4 cut(s) 85, 509, 1427, 1597
BstNSI RCATGY 1 cut(s) 1583
BstSCI CCNGG 7 cut(s) 83, 142, 160, 173, 507, 1425, 1595
BstSFI CTRYAG 2 cut(s) 641, 1119
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 1 cut(s) 1624
BstV1I GCAGC 7 cut(s) 105, 364, 466, 923, 1168, 1358, 1364
BstV2I GAAGAC 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 964
BstZI CGGCCG 1 cut(s) 1380
Bsu15I ATCGAT 1 cut(s) 784
Bsu36I CCTNAGG 2 cut(s) 633, 977
BsuI GTATCC 1 cut(s) 1629
BsuRI GGCC 4 cut(s) 52, 88, 183, 1382
BsuTUI ATCGAT 1 cut(s) 784
BtgI CCRYGG 1 cut(s) 1622
BtsCI GGATG 5 cut(s) 450, 553, 690, 1257, 1421
BtsI GCAGTG 3 cut(s) 429, 1173, 1374
BtsIMutI CAGTG 7 cut(s) 351, 429, 868, 881, 1173, 1355, 1374
BveI ACCTGC 2 cut(s) 1211, 1318
Cac8I GCNNGC 4 cut(s) 71, 1179, 1497, 1501
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 7 cut(s) 50, 51, 87, 514, 878, 902, 938
Cfr42I CCGCGG 1 cut(s) 1625
ClaI ATCGAT 1 cut(s) 784
CseI GACGC 1 cut(s) 845
Csp6I GTAC 3 cut(s) 761, 1124, 1273
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 5 cut(s) 383, 872, 994, 1396, 1580
CviQI GTAC 3 cut(s) 761, 1124, 1273
DdeI CTNAG 5 cut(s) 633, 945, 977, 1127, 1344
DpnI GATC 5 cut(s) 63, 582, 798, 888, 1246
DpnII GATC 5 cut(s) 61, 580, 796, 886, 1244
DraI TTTAAA 1 cut(s) 1339
EaeI YGGCCR 2 cut(s) 181, 1380
EagI CGGCCG 1 cut(s) 1380
EclXI CGGCCG 1 cut(s) 1380
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 2 cut(s) 54, 75
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 4 cut(s) 514, 878, 902, 938
Eco52I CGGCCG 1 cut(s) 1380
Eco57I CTGAAG 1 cut(s) 1179
Eco81I CCTNAGG 2 cut(s) 633, 977
EcoO109I RGGNCCY 1 cut(s) 938
EcoRII CCWGG 4 cut(s) 83, 507, 1425, 1595
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 1390
EcoT38I GRGCYC 2 cut(s) 54, 75
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 386, 875, 997, 1399, 1583
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FaqI GGGAC 3 cut(s) 130, 411, 500
FatI CATG 5 cut(s) 382, 871, 993, 1395, 1579
FauI CCCGC 1 cut(s) 450
FauNDI CATATG 1 cut(s) 907
FokI GGATG 5 cut(s) 457, 560, 677, 1264, 1408
FriOI GRGCYC 2 cut(s) 54, 75
FspBI CTAG 2 cut(s) 189, 1500
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 2 cut(s) 1076, 1504
HaeIII GGCC 4 cut(s) 52, 88, 183, 1382
HapII CCGG 5 cut(s) 109, 143, 162, 168, 174
HgaI GACGC 1 cut(s) 845
Hin1II CATG 5 cut(s) 386, 875, 997, 1399, 1583
HindIII AAGCTT 2 cut(s) 951, 1053
HinfI GANTC 7 cut(s) 32, 38, 118, 247, 601, 781, 1403
HpaII CCGG 5 cut(s) 109, 143, 162, 168, 174
Hpy166II GTNNAC 1 cut(s) 1226
Hpy188I TCNGA 7 cut(s) 37, 43, 61, 123, 252, 615, 1135
Hpy188III TCNNGA 6 cut(s) 575, 605, 922, 1093, 1330, 1407
Hpy8I GTNNAC 1 cut(s) 1226
HpyAV CCTTC 5 cut(s) 285, 616, 929, 1130, 1552
HpyCH4III ACNGT 2 cut(s) 645, 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 377, 383, 950, 1385
HpyF3I CTNAG 5 cut(s) 633, 945, 977, 1127, 1344
Hsp92II CATG 5 cut(s) 386, 875, 997, 1399, 1583
KspI CCGCGG 1 cut(s) 1625
Kzo9I GATC 5 cut(s) 61, 580, 796, 886, 1244
LmnI GCTCC 3 cut(s) 126, 177, 1036
Lsp1109I GCAGC 7 cut(s) 105, 364, 466, 923, 1168, 1358, 1364
LweI GCATC 3 cut(s) 673, 1021, 1375
MaeI CTAG 2 cut(s) 189, 1500
MaeIII GTNAC 8 cut(s) 146, 353, 427, 676, 857, 1285, 1429, 1628
MalI GATC 5 cut(s) 63, 582, 798, 888, 1246
MbiI CCGCTC 1 cut(s) 180
MboI GATC 5 cut(s) 61, 580, 796, 886, 1244
MboII GAAGA 4 cut(s) 56, 349, 836, 1172
MhlI GDGCHC 2 cut(s) 54, 75
MluCI AATT 9 cut(s) 55, 521, 557, 652, 987, 1016, 1280, 1333, 1415
MlyI GAGTC 2 cut(s) 112, 610
MmeI TCCRAC 1 cut(s) 1029
Mph1103I ATGCAT 1 cut(s) 1390
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 5 cut(s) 231, 591, 650, 747, 1338
MspA1I CMGCKG 2 cut(s) 457, 1624
MspI CCGG 5 cut(s) 109, 143, 162, 168, 174
MspR9I CCNGG 7 cut(s) 85, 144, 162, 175, 509, 1427, 1597
MvaI CCWGG 4 cut(s) 85, 509, 1427, 1597
MvnI CGCG 1 cut(s) 1624
MwoI GCNNNNNNNGC 5 cut(s) 177, 377, 383, 950, 1385
NciI CCSGG 3 cut(s) 144, 162, 175
NdeI CATATG 1 cut(s) 907
NdeII GATC 5 cut(s) 61, 580, 796, 886, 1244
NheI GCTAGC 1 cut(s) 1499
NlaIII CATG 5 cut(s) 386, 875, 997, 1399, 1583
NlaIV GGNNCC 5 cut(s) 52, 128, 166, 172, 516
NmuCI GTSAC 6 cut(s) 146, 427, 676, 857, 1285, 1628
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 1 cut(s) 1583
PaqCI CACCTGC 1 cut(s) 1318
PasI CCCWGGG 1 cut(s) 1596
PciI ACATGT 1 cut(s) 1579
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 5 cut(s) 32, 38, 247, 781, 1403
PleI GAGTC 2 cut(s) 112, 609
PpsI GAGTC 2 cut(s) 112, 609
PpuMI RGGWCCY 1 cut(s) 938
PscI ACATGT 1 cut(s) 1579
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 4 cut(s) 83, 507, 1425, 1595
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 4 cut(s) 83, 507, 1425, 1595
PspN4I GGNNCC 5 cut(s) 52, 128, 166, 172, 516
PspOMI GGGCCC 1 cut(s) 50
PspPI GGNCC 7 cut(s) 50, 51, 87, 514, 878, 902, 938
PspPPI RGGWCCY 1 cut(s) 938
PstI CTGCAG 1 cut(s) 1123
RsaI GTAC 3 cut(s) 762, 1125, 1274
RsaNI GTAC 3 cut(s) 761, 1124, 1273
SacII CCGCGG 1 cut(s) 1625
SaqAI TTAA 5 cut(s) 231, 591, 650, 747, 1338
Sau3AI GATC 5 cut(s) 61, 580, 796, 886, 1244
Sau96I GGNCC 7 cut(s) 50, 51, 87, 514, 878, 902, 938
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 2 cut(s) 112, 610
ScrFI CCNGG 7 cut(s) 85, 144, 162, 175, 509, 1427, 1597
SduI GDGCHC 2 cut(s) 54, 75
SfaNI GCATC 3 cut(s) 673, 1021, 1375
SfcI CTRYAG 2 cut(s) 641, 1119
Sfr303I CCGCGG 1 cut(s) 1625
SgrBI CCGCGG 1 cut(s) 1625
SinI GGWCC 4 cut(s) 514, 878, 902, 938
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 9 cut(s) 55, 521, 557, 652, 987, 1016, 1280, 1333, 1415
SspMI CTAG 2 cut(s) 189, 1500
StyD4I CCNGG 7 cut(s) 83, 142, 160, 173, 507, 1425, 1595
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 645, 863
TaqI TCGA 2 cut(s) 784, 1060
TasI AATT 9 cut(s) 55, 521, 557, 652, 987, 1016, 1280, 1333, 1415
TatI WGTACW 2 cut(s) 760, 1123
TauI GCSGC 4 cut(s) 83, 132, 183, 1382
TfiI GAWTC 5 cut(s) 32, 38, 247, 781, 1403
Tru1I TTAA 5 cut(s) 231, 591, 650, 747, 1338
Tru9I TTAA 5 cut(s) 231, 591, 650, 747, 1338
TscAI CASTG 7 cut(s) 351, 436, 868, 888, 1173, 1362, 1381
TseFI GTSAC 6 cut(s) 146, 427, 676, 857, 1285, 1628
TseI GCWGC 7 cut(s) 93, 377, 454, 911, 1181, 1352, 1371
Tsp45I GTSAC 6 cut(s) 146, 427, 676, 857, 1285, 1628
TspDTI ATGAA 6 cut(s) 92, 254, 275, 461, 948, 1354
TspGWI ACGGA 1 cut(s) 1504
TspRI CASTG 7 cut(s) 351, 436, 868, 888, 1173, 1362, 1381
VpaK11BI GGWCC 4 cut(s) 514, 878, 902, 938
XapI RAATTY 4 cut(s) 521, 652, 1016, 1333
XceI RCATGY 1 cut(s) 1583
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 2 cut(s) 189, 1500
ZrmI AGTACT 1 cut(s) 1125
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.