RchiOBHm_Chr5g0002251

DUF246 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002069
Physical Location & Seq
Reverse (-)
1311113 .. 1313385
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28363

Sequence Viewer

Length: 783 bp
ATGAATGGGTATCTAATGGTCACTGCAAATGGTGGGATGAACCAGCAGCCGGTTGCGGTAAGTGATGGTTTGCATTGCAATATTGTTGTTATTGCACAATTACTCAATGCAACCCTGGTTGTCCCCAAATATTTGTATAGTAGTATATATATGGAGGGATGTAAGGAGGATCATTTTATTAACTACTTGAGTCCTGATATTCGGATAGTGAAGGAACTCCCTAAGGAACTACAGTCATTAAATTTGGAAGCTATTGGTAGTCTTGTGACAGATGCAGACATCCCTAAAGAGGCAAAGCCAAGTTTTTACTTGAAAAACATTACTCCTATTCTACTTAAAAATGGGGTTGTACATTTTCTTGGATTTGGGAATCGATTGTCATTTGATCCAATACCATTTCAGTTGCAGATGTGGAGCAAAAGGAGTTGGAAGCTTTTTGAGAAATCCATTTCCCTGCATTGGTGCTTCTCCAGAAGACTACAATTAAAGTATGCAGTATTATATGATTTATATCATTTGGTTGGCTACCTACTCCTGCATTATTCAGGTCCTTTATCGCCTGAAGAAGCTATGCTTATGCTTGCTGCTCTTGGTTTCAAGTGTAAGACAAATATTTGTGGCAGGTTCACAGATATATGGAGGGAGTGTGAAAACCATATATACATGCTCACAACATATGCACATCAACTTGACATCAATCACAAAAGAACTAAAACTTACCTTCAGCAATCACAGACATGGATTCCATCTTCTGCAGCTGCAAACTTGATCACAAGAATATAG

Protein Analysis

260

Amino Acids

29.79

Weight (kDa)

8.79

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 4 - 146 2.9e-24 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 612
AciI CCGC 1 cut(s) 56
AclWI GGATC 2 cut(s) 177, 380
AcsI RAATTY 1 cut(s) 241
AcuI CTGAAG 2 cut(s) 582, 707
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 3 cut(s) 49, 289, 459
AgsI TTSAA 2 cut(s) 313, 598
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 4 cut(s) 251, 433, 569, 758
AluI AGCT 4 cut(s) 251, 433, 569, 758
AlwI GGATC 2 cut(s) 177, 380
ApeKI GCWGC 4 cut(s) 46, 584, 755, 758
ApoI RAATTY 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 548
AvaII GGWCC 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 481
BbvI GCAGC 4 cut(s) 58, 571, 745, 767
BccI CCATC 2 cut(s) 59, 754
BciT130I CCWGG 1 cut(s) 116
BclI TGATCA 1 cut(s) 768
BfmI CTRYAG 2 cut(s) 230, 753
BfuAI ACCTGC 1 cut(s) 612
BisI GCNGC 4 cut(s) 47, 585, 756, 759
BlsI GCNGC 4 cut(s) 48, 586, 757, 760
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 373
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 289, 459
Bse118I RCCGGY 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 3 cut(s) 42, 164, 279
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 49, 289, 459
BseMI GCAATG 1 cut(s) 73
BseXI GCAGC 4 cut(s) 58, 571, 745, 767
BshVI ATCGAT 1 cut(s) 373
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 49, 289, 459
BsmFI GGGAC 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 169, 385, 768
BspACI CCGC 1 cut(s) 56
BspDI ATCGAT 1 cut(s) 373
BspMAI CTGCAG 1 cut(s) 757
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 2 cut(s) 177, 380
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 3 cut(s) 169, 385, 768
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 42, 164, 279
BstKTI GATC 3 cut(s) 172, 388, 771
BstMBI GATC 3 cut(s) 169, 385, 768
BstNI CCWGG 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 667
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 2 cut(s) 230, 753
BstV1I GCAGC 4 cut(s) 58, 571, 745, 767
BstV2I GAAGAC 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 373
Bsu36I CCTNAGG 1 cut(s) 222
BsuTUI ATCGAT 1 cut(s) 373
BtsCI GGATG 3 cut(s) 42, 164, 279
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 1 cut(s) 582
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 548
ClaI ATCGAT 1 cut(s) 373
Csp6I GTAC 1 cut(s) 350
CviAII CATG 2 cut(s) 664, 738
CviJI RGCY 7 cut(s) 49, 251, 298, 433, 525, 569, 758
CviKI_1 RGCY 7 cut(s) 49, 251, 298, 433, 525, 569, 758
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 1 cut(s) 222
DpnI GATC 3 cut(s) 171, 387, 770
DpnII GATC 3 cut(s) 169, 385, 768
Eco47I GGWCC 1 cut(s) 548
Eco57I CTGAAG 2 cut(s) 582, 707
Eco81I CCTNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 114
FaeI CATG 2 cut(s) 667, 741
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 1 cut(s) 107
FatI CATG 2 cut(s) 663, 737
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 1 cut(s) 768
Fnu4HI GCNGC 4 cut(s) 47, 585, 756, 759
FokI GGATG 3 cut(s) 49, 171, 266
Fsp4HI GCNGC 4 cut(s) 47, 585, 756, 759
GluI GCNGC 4 cut(s) 47, 585, 756, 759
GsuI CTGGAG 1 cut(s) 454
HapII CCGG 1 cut(s) 50
Hin1II CATG 2 cut(s) 667, 741
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 3 cut(s) 190, 370, 742
HpaII CCGG 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 2 cut(s) 194, 471
Hpy8I GTNNAC 1 cut(s) 627
HpyAV CCTTC 2 cut(s) 205, 731
HpyCH4III ACNGT 1 cut(s) 234
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 2 cut(s) 667, 741
Ksp22I TGATCA 1 cut(s) 768
Kzo9I GATC 3 cut(s) 169, 385, 768
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 4 cut(s) 58, 571, 745, 767
LweI GCATC 1 cut(s) 262
MaeIII GTNAC 2 cut(s) 19, 265
MalI GATC 3 cut(s) 171, 387, 770
MboI GATC 3 cut(s) 169, 385, 768
MboII GAAGA 3 cut(s) 486, 575, 741
MluCI AATT 3 cut(s) 98, 241, 482
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 407
MnlI CCTC 4 cut(s) 148, 160, 283, 633
MseI TTAA 4 cut(s) 180, 239, 336, 485
MslI CAYNNNNRTG 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
NdeI CATATG 1 cut(s) 676
NdeII GATC 3 cut(s) 169, 385, 768
NlaIII CATG 2 cut(s) 667, 741
NmuCI GTSAC 2 cut(s) 19, 265
NspI RCATGY 1 cut(s) 667
PfeI GAWTC 2 cut(s) 370, 742
PkrI GCNGC 4 cut(s) 48, 586, 757, 760
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
PpuMI RGGWCCY 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspPI GGNCC 1 cut(s) 548
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 757
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
RseI CAYNNNNRTG 1 cut(s) 736
SaqAI TTAA 4 cut(s) 180, 239, 336, 485
SatI GCNGC 4 cut(s) 47, 585, 756, 759
Sau3AI GATC 3 cut(s) 169, 385, 768
Sau96I GGNCC 1 cut(s) 548
SchI GAGTC 1 cut(s) 199
ScrFI CCNGG 1 cut(s) 116
SetI ASST 8 cut(s) 253, 435, 531, 550, 571, 626, 723, 760
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 2 cut(s) 230, 753
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 3 cut(s) 98, 241, 482
SsiI CCGC 1 cut(s) 56
SspI AATATT 3 cut(s) 82, 131, 613
StyD4I CCNGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 373
TasI AATT 3 cut(s) 98, 241, 482
TatI WGTACW 1 cut(s) 349
TfiI GAWTC 2 cut(s) 370, 742
Tru1I TTAA 4 cut(s) 180, 239, 336, 485
Tru9I TTAA 4 cut(s) 180, 239, 336, 485
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 2 cut(s) 19, 265
TseI GCWGC 4 cut(s) 46, 584, 755, 758
Tsp45I GTSAC 2 cut(s) 19, 265
TspDTI ATGAA 2 cut(s) 17, 53
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 1 cut(s) 241
XceI RCATGY 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.