RchiOBHm_Chr5g0002271

DUF246 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002069
Physical Location & Seq
Reverse (-)
1318182 .. 1322408
4227 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28365

Sequence Viewer

Length: 1944 bp
ATGTCCCTCACCGCCGTCCAAGGACCCCCTGAATCCGAATCGGAAGACGGGCCCGATTCAGATCTGGGCGAGCCCAACGCCGCCTGGCCCAGCAGCTCGTTCCATTTCCGGTCGGGGACTCCTAGGAGCCGCCACTCCTCGCCGGGTCACTCGCCCCGACCCGGCACCGGCACCGGCACCGGCCACGGCCACACTAGGTTCGGCGACGCGGATTCCCAAACGACGTCGTTTTGGGGGGGACTGAGTGCGCAATGGAGATCGGGGACGAGGAAAGTGGAGAGGGCTTGGTATAGCAAAAAGAGAGTGAAAGGGATTGTGGGTGTGATTGGGCTGTTGGTTTTGTACTTTGTTATGAATTGGGTTATGCTTTTGCGGCTACAAGACCACCGCCTTCATAGCAAGTCTTGGTTTTCCGGCAAATCTTCATCGTCTCCGGCGCCCATCTCGGAAAAGTCAAAAAAATATGGTAAAGGGAAAAAGCCAGTGAAAGGTTACGAAAGAATGTTGGCTTTTGCTGCTCATGCCTTGGCAGAGGGACATAGTAAGCCCGAGCCAAAAGATTTGTGGCAGGAGCCATTTGTTCCTGCTTCTTCTTGGAAACCTTGTGCGGACCAACGCAGCTGGGAACCCAATGAGGGAAAGAATGGATATATAATGGTCACTGCAAATGGTGGGATGAACCAGCAGCGGGTTGCGGTTTGCAACATTGTTGTTATTGCACGATTACTCAATGCAACCCTGGTGGTCCCCAAATTTTTGTATAGTAGTATATGGAGGGATGTAAGTCAATTCAGCGATATCTATCAGGAGGATCATTTTATTAACTACTTGAGTCCTGATATTCGGATAGTGAAGGAACTCCCTAAGGAACTACAATCATTAAATTTGGAAGCTATTGGTAGCCTTGTGACAGATGCAGACATCCCTAAAGAGGCAAAGCCAAGTTTTTACTTGAAAAACATTACCCCTATTCTACTTAAAAATGGGGTTGTACATTTTCTTGGATTTGGGAATCGATTGGCATTTGATCCAATACCATTTCAGTTGCAGAGACTTCGATGCAGATGTAATTTTCATTCCCTGCAATTTGTTCCGAAGATACAAAAAATGGGTGCATTACTTCTTCAAAGGTTGCGTCACAGTGAAAGTCATGCTGGACCACTGGATCATTATCTGGTTGGTCCATATGCAGCACCAATCAAGAAAGAAAATGAAGGTCCTGCTAAGAAAGCTTCCAAGTATCTGGCTTTACACCTGAGGTTTGAAATTGACATGGTAGCCCATTCTTTGTGTGAATTTGGCGGAGGAGATGCGGAGCAAAAGGAGTTGGAAGCTTTTCGACAAATCCATTTCCCTGCATTGGTGCTTCTCCAGAATACTACAAGGCTACCTACTCCTGCAGTACTCAGGTCAGAAGGCTTGTGTCCTTTGTCGCCTGAAGAAGCAGTGCTTATGCTTGCTGCTCTTGGTTTCAAGCGTAAGACACATATATTTGTGGCAGGTTCACAAATATATGGAGGGAGATCAAGGATGATTGCTTTGACGAGCTTGTACCCTAAATTGGTGACTAAAGAAAACTTGCTTTCACCCGCTGAACTTGAACCTTTCAAGAATTTTAAATCTCAGTTAGCAGCACTGGACTTCATAGCCTGCACTGCTGCCGATGCATTTGCCATGACTGATTCTGGAAGTCAATTATCATCCCTGGTAACTGGTTTTCGCATATACTATGGTGGAGGGATAACACCAACAATACGACCAAACAAACGGAGGCTTGCCAGCATTTTTTCAAAGAAATCCAGTATTGAATGGCGTGTGTTTGAGCAGAGAGTGAGGAAGGCAGTTAGACAAACCAAACATGTTCAGCCAAGACCCAGGGCAAGGAGTGTTTATCGGTATCCGCGGTGTGACGAGTGTATGTGTAATATGACATTAGTGATATAA

Protein Analysis

647

Amino Acids

72.26

Weight (kDa)

9.7

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 217 - 577 1.8e-95 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
Acc16I TGCGCA 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 1490
AccB1I GGYRCC 4 cut(s) 164, 170, 176, 436
AccII CGCG 2 cut(s) 209, 1903
AclWI GGATC 3 cut(s) 819, 1022, 1173
AcoI YGGCCR 2 cut(s) 181, 187
AcsI RAATTY 4 cut(s) 752, 883, 1295, 1612
AcuI CTGAAG 1 cut(s) 1458
AcyI GRCGYC 2 cut(s) 224, 437
AfaI GTAC 4 cut(s) 344, 993, 1404, 1553
AfiI CCNNNNNNNGG 9 cut(s) 161, 167, 488, 635, 688, 931, 1360, 1561, 1881
AflIII ACRYGT 1 cut(s) 1858
AgsI TTSAA 8 cut(s) 955, 1127, 1265, 1474, 1601, 1609, 1791, 1808
AjnI CCWGG 4 cut(s) 83, 738, 1704, 1874
AluBI AGCT 6 cut(s) 96, 621, 893, 1232, 1334, 1548
AluI AGCT 6 cut(s) 96, 621, 893, 1232, 1334, 1548
Alw26I GTCTC 2 cut(s) 435, 1045
AlwI GGATC 3 cut(s) 819, 1022, 1173
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 4 cut(s) 50, 86, 181, 187
ApaI GGGCCC 1 cut(s) 54
ApeKI GCWGC 8 cut(s) 93, 515, 618, 685, 1190, 1460, 1631, 1658
ApoI RAATTY 4 cut(s) 752, 883, 1295, 1612
ArsI GACNNNNNNTTYG 2 cut(s) 1120, 1152
Asp700I GAANNNNTTC 1 cut(s) 1335
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 2 cut(s) 250, 439
AspS9I GGNCC 9 cut(s) 23, 50, 51, 87, 610, 745, 1157, 1181, 1217
AsuC2I CCSGG 2 cut(s) 144, 162
AsuHPI GGTGA 2 cut(s) 1576, 1578
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 6 cut(s) 23, 610, 745, 1157, 1181, 1217
AvrII CCTAGG 1 cut(s) 122
AxyI CCTNAGG 2 cut(s) 864, 1256
BaeGI GKGCMC 1 cut(s) 54
BanI GGYRCC 4 cut(s) 164, 170, 176, 436
BanII GRGCYC 2 cut(s) 54, 75
BbsI GAAGAC 1 cut(s) 51
BbvI GCAGC 8 cut(s) 105, 502, 630, 697, 1202, 1447, 1643, 1645
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 202
BcgI CGANNNNNNTGC 4 cut(s) 1037, 1071, 1652, 1686
BciT130I CCWGG 4 cut(s) 85, 740, 1706, 1876
BciVI GTATCC 1 cut(s) 1908
BcnI CCSGG 2 cut(s) 144, 162
BcoDI GTCTC 2 cut(s) 435, 1045
BfaI CTAG 2 cut(s) 123, 195
BfmI CTRYAG 1 cut(s) 1398
BfoI RGCGCY 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 1490
BfuI GTATCC 1 cut(s) 1908
BglII AGATCT 1 cut(s) 61
BlnI CCTAGG 1 cut(s) 122
BmcAI AGTACT 1 cut(s) 1404
Bme1390I CCNGG 6 cut(s) 85, 144, 162, 740, 1706, 1876
Bme18I GGWCC 6 cut(s) 23, 610, 745, 1157, 1181, 1217
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 9 cut(s) 23, 50, 51, 87, 610, 745, 1157, 1181, 1217
BmrFI CCNGG 6 cut(s) 85, 144, 162, 740, 1706, 1876
BmsI GCATC 4 cut(s) 904, 1049, 1300, 1654
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 1355
BpuEI CTTGAG 1 cut(s) 850
BpuMI CCSGG 2 cut(s) 144, 162
Bsa29I ATCGAT 1 cut(s) 1015
BsaBI GATNNNNATC 3 cut(s) 60, 801, 1170
BsaHI GRCGYC 2 cut(s) 224, 437
BsaJI CCNNGG 9 cut(s) 19, 122, 184, 525, 738, 1704, 1874, 1875, 1901
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 9 cut(s) 161, 167, 488, 635, 688, 931, 1360, 1561, 1881
Bse118I RCCGGY 3 cut(s) 167, 173, 179
Bse1I ACTGG 5 cut(s) 482, 1167, 1641, 1717, 1800
Bse21I CCTNAGG 2 cut(s) 864, 1256
Bse3DI GCAATG 1 cut(s) 257
Bse8I GATNNNNATC 3 cut(s) 60, 801, 1170
BseBI CCWGG 4 cut(s) 85, 740, 1706, 1876
BseCI ATCGAT 1 cut(s) 1015
BseDI CCNNGG 9 cut(s) 19, 122, 184, 525, 738, 1704, 1874, 1875, 1901
BseGI GGATG 5 cut(s) 681, 784, 921, 1536, 1700
BseJI GATNNNNATC 3 cut(s) 60, 801, 1170
BseLI CCNNNNNNNGG 9 cut(s) 161, 167, 488, 635, 688, 931, 1360, 1561, 1881
BseMI GCAATG 1 cut(s) 257
BseMII CTCAG 4 cut(s) 233, 1247, 1420, 1637
BseNI ACTGG 5 cut(s) 482, 1167, 1641, 1717, 1800
BseRI GAGGAG 2 cut(s) 127, 1320
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 8 cut(s) 105, 502, 630, 697, 1202, 1447, 1643, 1645
BseYI CCCAGC 2 cut(s) 89, 621
BsgI GTGCAG 1 cut(s) 1636
Bsh1236I CGCG 2 cut(s) 209, 1903
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 52, 88, 183, 189
BshNI GGYRCC 4 cut(s) 164, 170, 176, 436
BshVI ATCGAT 1 cut(s) 1015
BsiEI CGRYCG 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 8 cut(s) 109, 143, 162, 168, 174, 180, 414, 434
BslFI GGGAC 5 cut(s) 130, 252, 277, 549, 731
BslI CCNNNNNNNGG 9 cut(s) 161, 167, 488, 635, 688, 931, 1360, 1561, 1881
BsmAI GTCTC 2 cut(s) 435, 1045
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 5 cut(s) 130, 252, 277, 549, 731
BsnI GGCC 4 cut(s) 52, 88, 183, 189
BsoBI CYCGRG 1 cut(s) 548
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 54, 75
Bsp1407I TGTACA 1 cut(s) 991
Bsp143I GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
BspANI GGCC 4 cut(s) 52, 88, 183, 189
BspCNI CTCAG 4 cut(s) 234, 1248, 1419, 1636
BspDI ATCGAT 1 cut(s) 1015
BspFNI CGCG 2 cut(s) 209, 1903
BspMAI CTGCAG 1 cut(s) 1402
BspMI ACCTGC 1 cut(s) 1490
BspPI GGATC 3 cut(s) 819, 1022, 1173
BspT107I GGYRCC 4 cut(s) 164, 170, 176, 436
BsrDI GCAATG 1 cut(s) 257
BsrFI RCCGGY 3 cut(s) 167, 173, 179
BsrGI TGTACA 1 cut(s) 991
BsrI ACTGG 5 cut(s) 482, 1167, 1641, 1717, 1800
BssAI RCCGGY 3 cut(s) 167, 173, 179
BssECI CCNNGG 9 cut(s) 19, 122, 184, 525, 738, 1704, 1874, 1875, 1901
BssMI GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
BssNI GRCGYC 2 cut(s) 224, 437
BssT1I CCWWGG 3 cut(s) 19, 122, 525
Bst2UI CCWGG 4 cut(s) 85, 740, 1706, 1876
Bst4CI ACNGT 1 cut(s) 1142
BstACI GRCGYC 2 cut(s) 224, 437
BstAUI TGTACA 1 cut(s) 991
BstC8I GCNNGC 5 cut(s) 71, 1458, 1651, 1776, 1780
BstDEI CTNAG 6 cut(s) 242, 864, 1224, 1256, 1406, 1623
BstDSI CCRYGG 2 cut(s) 184, 1901
BstF5I GGATG 5 cut(s) 681, 784, 921, 1536, 1700
BstFNI CGCG 2 cut(s) 209, 1903
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 250, 439
BstKTI GATC 6 cut(s) 64, 260, 814, 1030, 1168, 1526
BstMAI GTCTC 2 cut(s) 435, 1045
BstMBI GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 7 cut(s) 373, 396, 515, 521, 1229, 1655, 1664
BstNI CCWGG 4 cut(s) 85, 740, 1706, 1876
BstNSI RCATGY 1 cut(s) 1862
BstSCI CCNGG 6 cut(s) 83, 142, 160, 738, 1704, 1874
BstSFI CTRYAG 1 cut(s) 1398
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 2 cut(s) 209, 1903
BstV1I GCAGC 8 cut(s) 105, 502, 630, 697, 1202, 1447, 1643, 1645
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 1243
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 1015
Bsu36I CCTNAGG 2 cut(s) 864, 1256
BsuI GTATCC 1 cut(s) 1908
BsuRI GGCC 4 cut(s) 52, 88, 183, 189
BsuTUI ATCGAT 1 cut(s) 1015
BtgI CCRYGG 2 cut(s) 184, 1901
BtsCI GGATG 5 cut(s) 681, 784, 921, 1536, 1700
BtsI GCAGTG 3 cut(s) 660, 1452, 1653
BtsIMutI CAGTG 7 cut(s) 489, 660, 1147, 1160, 1452, 1634, 1653
BveI ACCTGC 1 cut(s) 1490
Cac8I GCNNGC 5 cut(s) 71, 1458, 1651, 1776, 1780
CfoI GCGC 2 cut(s) 250, 439
Cfr10I RCCGGY 3 cut(s) 167, 173, 179
Cfr13I GGNCC 9 cut(s) 23, 50, 51, 87, 610, 745, 1157, 1181, 1217
Cfr42I CCGCGG 1 cut(s) 1904
ClaI ATCGAT 1 cut(s) 1015
CseI GACGC 2 cut(s) 215, 1124
Csp6I GTAC 4 cut(s) 343, 992, 1403, 1552
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 5 cut(s) 521, 1151, 1273, 1675, 1859
CviQI GTAC 4 cut(s) 343, 992, 1403, 1552
DdeI CTNAG 6 cut(s) 242, 864, 1224, 1256, 1406, 1623
DinI GGCGCC 1 cut(s) 438
DpnI GATC 6 cut(s) 63, 259, 813, 1029, 1167, 1525
DpnII GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
DraI TTTAAA 1 cut(s) 1618
EaeI YGGCCR 2 cut(s) 181, 187
EciI GGCGGA 1 cut(s) 1317
Eco130I CCWWGG 3 cut(s) 19, 122, 525
Eco24I GRGCYC 2 cut(s) 54, 75
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 6 cut(s) 23, 610, 745, 1157, 1181, 1217
Eco57I CTGAAG 1 cut(s) 1458
Eco81I CCTNAGG 2 cut(s) 864, 1256
Eco88I CYCGRG 1 cut(s) 548
EcoO109I RGGNCCY 2 cut(s) 23, 1217
EcoRII CCWGG 4 cut(s) 83, 738, 1704, 1874
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 3 cut(s) 19, 122, 525
EcoT22I ATGCAT 1 cut(s) 1669
EcoT38I GRGCYC 2 cut(s) 54, 75
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 3 cut(s) 19, 122, 525
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 5 cut(s) 524, 1154, 1276, 1678, 1862
FalI AAGNNNNNCTT 2 cut(s) 1434, 1466
FaqI GGGAC 5 cut(s) 130, 252, 277, 549, 731
FatI CATG 5 cut(s) 520, 1150, 1272, 1674, 1858
FauI CCCGC 2 cut(s) 681, 1597
FauNDI CATATG 1 cut(s) 1186
FokI GGATG 5 cut(s) 688, 791, 908, 1543, 1687
FriOI GRGCYC 2 cut(s) 54, 75
FspBI CTAG 2 cut(s) 123, 195
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 2 cut(s) 249, 438
GsaI CCCAGC 2 cut(s) 93, 625
GsuI CTGGAG 1 cut(s) 1355
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 4 cut(s) 52, 88, 183, 189
HapII CCGG 8 cut(s) 109, 143, 162, 168, 174, 180, 414, 434
HgaI GACGC 2 cut(s) 215, 1124
HhaI GCGC 2 cut(s) 250, 439
Hin1I GRCGYC 2 cut(s) 224, 437
Hin1II CATG 5 cut(s) 524, 1154, 1276, 1678, 1862
Hin6I GCGC 2 cut(s) 248, 437
HinP1I GCGC 2 cut(s) 248, 437
HindIII AAGCTT 2 cut(s) 1230, 1332
HinfI GANTC 8 cut(s) 32, 38, 56, 118, 212, 832, 1012, 1682
HpaII CCGG 8 cut(s) 109, 143, 162, 168, 174, 180, 414, 434
HphI GGTGA 2 cut(s) 1576, 1578
Hpy166II GTNNAC 1 cut(s) 1505
Hpy188I TCNGA 7 cut(s) 37, 43, 61, 448, 846, 1095, 1414
Hpy188III TCNNGA 6 cut(s) 806, 836, 1201, 1372, 1609, 1686
Hpy8I GTNNAC 1 cut(s) 1505
Hpy99I CGWCG 3 cut(s) 209, 226, 229
HpyAV CCTTC 5 cut(s) 401, 847, 1208, 1409, 1831
HpyCH4III ACNGT 1 cut(s) 1142
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 7 cut(s) 373, 396, 515, 521, 1229, 1655, 1664
HpyF3I CTNAG 6 cut(s) 242, 864, 1224, 1256, 1406, 1623
HpySE526I ACGT 1 cut(s) 224
Hsp92I GRCGYC 2 cut(s) 224, 437
Hsp92II CATG 5 cut(s) 524, 1154, 1276, 1678, 1862
HspAI GCGC 2 cut(s) 248, 437
KasI GGCGCC 1 cut(s) 436
KspI CCGCGG 1 cut(s) 1904
Kzo9I GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
LmnI GCTCC 3 cut(s) 126, 571, 1315
Lsp1109I GCAGC 8 cut(s) 105, 502, 630, 697, 1202, 1447, 1643, 1645
LweI GCATC 4 cut(s) 904, 1049, 1300, 1654
MaeI CTAG 2 cut(s) 123, 195
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 8 cut(s) 146, 491, 658, 907, 1136, 1564, 1708, 1907
MalI GATC 6 cut(s) 63, 259, 813, 1029, 1167, 1525
MboI GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
MboII GAAGA 6 cut(s) 56, 414, 582, 1108, 1115, 1451
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 54, 75
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 2 cut(s) 112, 841
MmeI TCCRAC 1 cut(s) 1308
Mph1103I ATGCAT 1 cut(s) 1669
MroXI GAANNNNTTC 1 cut(s) 1335
MseI TTAA 4 cut(s) 822, 881, 978, 1617
MspA1I CMGCKG 4 cut(s) 621, 688, 1592, 1903
MspI CCGG 8 cut(s) 109, 143, 162, 168, 174, 180, 414, 434
MspR9I CCNGG 6 cut(s) 85, 144, 162, 740, 1706, 1876
MvaI CCWGG 4 cut(s) 85, 740, 1706, 1876
MvnI CGCG 2 cut(s) 209, 1903
MwoI GCNNNNNNNGC 7 cut(s) 373, 396, 515, 521, 1229, 1655, 1664
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 2 cut(s) 144, 162
NdeI CATATG 1 cut(s) 1186
NdeII GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
NlaIII CATG 5 cut(s) 524, 1154, 1276, 1678, 1862
NmuCI GTSAC 6 cut(s) 146, 658, 907, 1136, 1564, 1907
NsbI TGCGCA 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 1669
NspI RCATGY 1 cut(s) 1862
PasI CCCWGGG 1 cut(s) 1875
PciI ACATGT 1 cut(s) 1858
PdmI GAANNNNTTC 1 cut(s) 1335
PfeI GAWTC 6 cut(s) 32, 38, 56, 212, 1012, 1682
PleI GAGTC 2 cut(s) 112, 840
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 2 cut(s) 112, 840
PpuMI RGGWCCY 2 cut(s) 23, 1217
PscI ACATGT 1 cut(s) 1858
Psp5II RGGWCCY 2 cut(s) 23, 1217
Psp6I CCWGG 4 cut(s) 83, 738, 1704, 1874
PspFI CCCAGC 2 cut(s) 89, 621
PspGI CCWGG 4 cut(s) 83, 738, 1704, 1874
PspOMI GGGCCC 1 cut(s) 50
PspPI GGNCC 9 cut(s) 23, 50, 51, 87, 610, 745, 1157, 1181, 1217
PspPPI RGGWCCY 2 cut(s) 23, 1217
PstI CTGCAG 1 cut(s) 1402
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 621
RsaI GTAC 4 cut(s) 344, 993, 1404, 1553
RsaNI GTAC 4 cut(s) 343, 992, 1403, 1552
SacII CCGCGG 1 cut(s) 1904
SaqAI TTAA 4 cut(s) 822, 881, 978, 1617
Sau3AI GATC 6 cut(s) 61, 257, 811, 1027, 1165, 1523
Sau96I GGNCC 9 cut(s) 23, 50, 51, 87, 610, 745, 1157, 1181, 1217
ScaI AGTACT 1 cut(s) 1404
SchI GAGTC 2 cut(s) 112, 841
ScrFI CCNGG 6 cut(s) 85, 144, 162, 740, 1706, 1876
SduI GDGCHC 2 cut(s) 54, 75
SfaNI GCATC 4 cut(s) 904, 1049, 1300, 1654
SfcI CTRYAG 1 cut(s) 1398
SfoI GGCGCC 1 cut(s) 438
Sfr303I CCGCGG 1 cut(s) 1904
SgrBI CCGCGG 1 cut(s) 1904
SinI GGWCC 6 cut(s) 23, 610, 745, 1157, 1181, 1217
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 2 cut(s) 123, 195
StyD4I CCNGG 6 cut(s) 83, 142, 160, 738, 1704, 1874
StyI CCWWGG 3 cut(s) 19, 122, 525
TaaI ACNGT 1 cut(s) 1142
TaiI ACGT 1 cut(s) 227
TaqI TCGA 3 cut(s) 1015, 1057, 1339
TatI WGTACW 3 cut(s) 342, 991, 1402
TauI GCSGC 3 cut(s) 83, 132, 376
TfiI GAWTC 6 cut(s) 32, 38, 56, 212, 1012, 1682
Tru1I TTAA 4 cut(s) 822, 881, 978, 1617
Tru9I TTAA 4 cut(s) 822, 881, 978, 1617
TscAI CASTG 7 cut(s) 489, 667, 1147, 1167, 1452, 1641, 1660
TseFI GTSAC 6 cut(s) 146, 658, 907, 1136, 1564, 1907
TseI GCWGC 8 cut(s) 93, 515, 618, 685, 1190, 1460, 1631, 1658
Tsp45I GTSAC 6 cut(s) 146, 658, 907, 1136, 1564, 1907
TspDTI ATGAA 7 cut(s) 368, 383, 414, 692, 1064, 1227, 1633
TspGWI ACGGA 1 cut(s) 1783
TspRI CASTG 7 cut(s) 489, 667, 1147, 1167, 1452, 1641, 1660
VpaK11BI GGWCC 6 cut(s) 23, 610, 745, 1157, 1181, 1217
XapI RAATTY 4 cut(s) 752, 883, 1295, 1612
XceI RCATGY 1 cut(s) 1862
XcmI CCANNNNNNNNNTGG 1 cut(s) 561
XmaJI CCTAGG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 1335
XspI CTAG 2 cut(s) 123, 195
ZraI GACGTC 1 cut(s) 225
ZrmI AGTACT 1 cut(s) 1404
Zsp2I ATGCAT 1 cut(s) 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.