MD10G1326100.v1.1

DUF246 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002069
Physical Location & Seq
Forward (+)
40641314 .. 40645040
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1326100.v1.1.491

Sequence Viewer

Length: 1947 bp
ATGTCCGTAAACATAGTTCAAATTGAACCAGTCCCACCAGAAGAAGATGGACACACCTCAGCTCAGCCCCTCGACCCCACCACCTCCTCCTCCGCCTATCCCAACCCCGCTTTGCTGCATTACCGGTCCGGCTCCTCCACTCCCGGGAGCCGCCCGTCATCCCCGACTAACTCACCGGGAAACGGCCTCACTCGGTTTTCCACCTCGGAGGCGCCACCGGATTGGGTGCCGAAGATGCAGAGGAGGACCTCCGCCAGGAAGGTGGGAGTTGAGAGGGCGTGGTATAGCAAAAAGCGAGTGAAGGGGATTGTGTGGGTGATTGGATTGTTGGTATTTTTCTTCTTGATGAATTGGGTTATGCTTTTGCGGCTTCAAGGTCATGGGATCCGTTCTGAATCCGGGTTACGCGGGAATTCGTCTCTGCCGTCGGTTTCCGTCACCATTCCGGAGAAATGGAGAAAAATTGGTAGAGTAAAAAAGGCACAGAAAGGCAATTATGGAAGGATGCTGGCTTTGGCCGCCCATGCATTGGCAGAGGGGCAGAGTAAACCTGAGCCGAAAGATCTGTGGCTGGAGCCATTAGTACCTTCTGTTTCTTGGAAACCTTGTGCCGACCAACGGAGCTGGGAACCTAATGAGGGAAAGAATGGATTCATTTTGGTCACTGCAAACGGTGGGATGAACCAGCAGCGAGTAGCTGTTTGCAATGCTGTCGCTGTTGCACGATTACTCAATTCAACTCTGGTTGTTCCCAAGTTTTTGTATAGTAGTATATGGAGAGATGTGAGTCAGTTCGGCGATATCTATCAGGAGGAGCATTTTATTAATTACTTGTCGCCTGATATTCGGATAGTGAAGGAACTCCCAAAGGAGCTACAGTCATTAGATTTGGAAGCAATTGGTAGTGTTGTGACAGATGTGGACATACCCAAAGAGGCAAAGCCAAGTTTTTACTTAAAACACATAATCCCCATTCTACTTAAGAATGGAGTCGTACATTTTCTTGGATTTGGGCATCGGTTGGCATTTGATCCAATACCATTTCAGTTGCAGAGACTTCGATGCAGATGTAATTTTCACGCCCTTCAATTTATCCCCAAGATACAAGAAGTTGGTGCTTTACTTCTTCAAAGACTGCGTCAAAATGAAACTCACCCTGGACCACTTGATCGTTATCTTGTTGGTTCATTTGCAGAACCAGTCAAGAAACAGACTAAAAGTCGTGCTAAGAAAGCTTCCAGATATCTAGCTTTACACCTGAGGTTTGAAATTGACATGGTAGCTCATTCTCTGTGTGAGTTTGGTGGAGGTGAGGAAGAGAGAAAAGAGTTGGAAGCGTATCGTGAAATCCATTTCCCCGCATTGACACTTCTCAAGAAGACTACAACGCTGCCTACTCCTGCAGAGCTCAGGGAAGAAGGCTTATGTCCCTTAACACCTGAAGAAGCAGTACTTATGCTTGCTGCTCTTGGTTTCAACAGGAAGACTCATGTATTTGTGGCAGGTTCACAGATATATGGAGGGAGATCAAGGATGATTGCTTTGACTAGCCTGTATCCGAAATTAGTGACCAAAGAAAATTTGCTCACACCGACTGAACTTGAACCTTTCATGAATTTTTCATCCCAGTTAGCAGCACTGGACTTCATAGGCTGCACTGCTGCCGATGCATTTGCCATGACCGATTCAGGAAGTCAGTTATCATCCTTGGTGTCTGGTTTTCGAATATACTATGGTGGAGGGAAAATGCCAACAATACGACCAAACAAACGCAGGCTTGCGAGCATTTTTATGAAGAATTCCACTATCGAATGGCGTGTTTTTGAGCAGAGAATTCGAAAGGCAGTTAGACAAACTAAACATATCCAACCAAGGCCTAAGGCGAGGAGTATGTATAGATATCCACGGTGCGACGAGTGCATGTGCAACATGACAATAATCTCCTGA

Protein Analysis

649

Amino Acids

72.79

Weight (kDa)

9.69

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 218 - 578 1.6e-96 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1493
AccB1I GGYRCC 2 cut(s) 211, 226
AccB7I CCANNNNNTGG 1 cut(s) 529
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 8 cut(s) 93, 108, 151, 252, 367, 408, 519, 1359
AclWI GGATC 3 cut(s) 379, 392, 1025
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 5 cut(s) 412, 1579, 1615, 1798, 1833
AcuI CTGAAG 1 cut(s) 1461
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 3 cut(s) 585, 996, 1452
AfiI CCNNNNNNNGG 6 cut(s) 144, 182, 255, 529, 618, 638
AflII CTTAAG 1 cut(s) 980
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 9 cut(s) 20, 26, 374, 738, 1088, 1130, 1268, 1477, 1604
AhdI GACNNNNNGTC 1 cut(s) 1218
AjnI CCWGG 2 cut(s) 254, 1156
AluBI AGCT 8 cut(s) 62, 624, 698, 874, 1235, 1250, 1283, 1408
AluI AGCT 8 cut(s) 62, 624, 698, 874, 1235, 1250, 1283, 1408
Alw21I GWGCWC 1 cut(s) 1410
Alw26I GTCTC 2 cut(s) 423, 1048
AlwI GGATC 3 cut(s) 379, 392, 1025
Ama87I CYCGRG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 3 cut(s) 184, 516, 1874
ApeKI GCWGC 7 cut(s) 115, 688, 1390, 1463, 1634, 1653, 1661
ApoI RAATTY 5 cut(s) 412, 1579, 1615, 1798, 1833
ArsI GACNNNNNNTTYG 2 cut(s) 1123, 1155
AseI ATTAAT 1 cut(s) 825
AsiGI ACCGGT 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 650
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 3 cut(s) 126, 246, 1160
AsuC2I CCSGG 4 cut(s) 144, 145, 177, 400
AsuHPI GGTGA 5 cut(s) 165, 328, 430, 1145, 1322
AsuII TTCGAA 2 cut(s) 1723, 1837
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 3 cut(s) 126, 246, 1160
AxyI CCTNAGG 2 cut(s) 1259, 1878
BamHI GGATCC 1 cut(s) 384
BanI GGYRCC 2 cut(s) 211, 226
BanII GRGCYC 1 cut(s) 1410
BarI GAAGNNNNNNTAC 2 cut(s) 1434, 1466
BbsI GAAGAC 2 cut(s) 1385, 1490
Bbv12I GWGCWC 1 cut(s) 1410
BbvCI CCTCAGC 1 cut(s) 58
BbvI GCAGC 7 cut(s) 102, 700, 1377, 1450, 1640, 1646, 1648
BccI CCATC 1 cut(s) 41
BceAI ACGGC 2 cut(s) 199, 409
BcgI CGANNNNNNTGC 8 cut(s) 694, 728, 1040, 1074, 1655, 1689, 1817, 1851
BciT130I CCWGG 2 cut(s) 256, 1158
BciVI GTATCC 1 cut(s) 1566
BcnI CCSGG 4 cut(s) 144, 145, 177, 400
BcoDI GTCTC 2 cut(s) 423, 1048
BfaI CTAG 2 cut(s) 1247, 1548
BfmI CTRYAG 2 cut(s) 875, 1401
BfoI RGCGCY 1 cut(s) 215
BfrI CTTAAG 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 1493
BfuI GTATCC 1 cut(s) 1566
BglII AGATCT 1 cut(s) 562
BlpI GCTNAGC 1 cut(s) 63
BmcAI AGTACT 1 cut(s) 1452
Bme1390I CCNGG 6 cut(s) 144, 145, 177, 256, 400, 1158
Bme18I GGWCC 3 cut(s) 126, 246, 1160
BmeRI GACNNNNNGTC 1 cut(s) 1218
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 126, 246, 1160
BmiI GGNNCC 7 cut(s) 133, 149, 213, 228, 386, 576, 630
BmrFI CCNGG 6 cut(s) 144, 145, 177, 256, 400, 1158
BmrI ACTGGG 1 cut(s) 1621
BmsI GCATC 5 cut(s) 225, 495, 1024, 1052, 1657
BmuI ACTGGG 1 cut(s) 1621
BpiI GAAGAC 2 cut(s) 1385, 1490
BpmI CTGGAG 1 cut(s) 593
Bpu10I CCTNAGC 3 cut(s) 58, 552, 1409
Bpu1102I GCTNAGC 1 cut(s) 63
Bpu14I TTCGAA 2 cut(s) 1723, 1837
BpuEI CTTGAG 1 cut(s) 1358
BpuMI CCSGG 4 cut(s) 144, 145, 177, 400
BsaBI GATNNNNATC 2 cut(s) 804, 1173
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 6 cut(s) 143, 204, 1156, 1707, 1871, 1904
BsaWI WCCGGW 3 cut(s) 123, 217, 445
BsaXI ACNNNNNCTCC 2 cut(s) 863, 893
Bsc4I CCNNNNNNNGG 6 cut(s) 144, 182, 255, 529, 618, 638
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 4 cut(s) 29, 1199, 1627, 1644
Bse21I CCTNAGG 2 cut(s) 1259, 1878
Bse3DI GCAATG 1 cut(s) 712
Bse8I GATNNNNATC 2 cut(s) 804, 1173
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 2 cut(s) 256, 1158
BseDI CCNNGG 6 cut(s) 143, 204, 1156, 1707, 1871, 1904
BseGI GGATG 6 cut(s) 158, 510, 684, 1539, 1622, 1703
BseJI GATNNNNATC 2 cut(s) 804, 1173
BseLI CCNNNNNNNGG 6 cut(s) 144, 182, 255, 529, 618, 638
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 5 cut(s) 72, 77, 543, 1250, 1423
BseNI ACTGG 4 cut(s) 29, 1199, 1627, 1644
BseRI GAGGAG 6 cut(s) 76, 79, 124, 256, 827, 1900
BseXI GCAGC 7 cut(s) 102, 700, 1377, 1450, 1640, 1646, 1648
BseYI CCCAGC 1 cut(s) 624
BsgI GTGCAG 1 cut(s) 1639
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 3 cut(s) 186, 518, 1876
BshNI GGYRCC 2 cut(s) 211, 226
BshTI ACCGGT 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 1410
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 7 cut(s) 124, 129, 144, 176, 218, 399, 446
BslFI GGGAC 2 cut(s) 17, 1413
BslI CCNNNNNNNGG 6 cut(s) 144, 182, 255, 529, 618, 638
BsmAI GTCTC 2 cut(s) 423, 1048
BsmBI CGTCTC 1 cut(s) 423
BsmFI GGGAC 2 cut(s) 17, 1413
BsnI GGCC 3 cut(s) 186, 518, 1876
BsoBI CYCGRG 1 cut(s) 143
Bsp119I TTCGAA 2 cut(s) 1723, 1837
Bsp1286I GDGCHC 1 cut(s) 1410
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 5 cut(s) 384, 562, 1030, 1168, 1526
Bsp1720I GCTNAGC 1 cut(s) 63
BspACI CCGC 8 cut(s) 93, 108, 151, 252, 367, 408, 519, 1359
BspANI GGCC 3 cut(s) 186, 518, 1876
BspCNI CTCAG 5 cut(s) 71, 76, 544, 1251, 1422
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 1611
BspLI GGNNCC 7 cut(s) 133, 149, 213, 228, 386, 576, 630
BspMAI CTGCAG 1 cut(s) 1405
BspMI ACCTGC 1 cut(s) 1493
BspPI GGATC 3 cut(s) 379, 392, 1025
BspT104I TTCGAA 2 cut(s) 1723, 1837
BspT107I GGYRCC 2 cut(s) 211, 226
BspTI CTTAAG 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 712
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 4 cut(s) 29, 1199, 1627, 1644
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 6 cut(s) 143, 204, 1156, 1707, 1871, 1904
BssMI GATC 5 cut(s) 384, 562, 1030, 1168, 1526
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 2 cut(s) 1707, 1871
Bst2UI CCWGG 2 cut(s) 256, 1158
Bst4CI ACNGT 3 cut(s) 674, 879, 1908
Bst6I CTCTTC 1 cut(s) 1311
BstACI GRCGYC 1 cut(s) 212
BstAFI CTTAAG 1 cut(s) 980
BstBI TTCGAA 2 cut(s) 1723, 1837
BstC8I GCNNGC 5 cut(s) 510, 1461, 1775, 1779, 1783
BstDEI CTNAG 7 cut(s) 58, 63, 552, 1227, 1259, 1409, 1878
BstDSI CCRYGG 1 cut(s) 1904
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 6 cut(s) 158, 510, 684, 1539, 1622, 1703
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 5 cut(s) 387, 565, 1033, 1171, 1529
BstMAI GTCTC 2 cut(s) 423, 1048
BstMBI GATC 5 cut(s) 384, 562, 1030, 1168, 1526
BstMWI GCNNNNNNNGC 7 cut(s) 235, 367, 518, 524, 1232, 1667, 1917
BstNI CCWGG 2 cut(s) 256, 1158
BstNSI RCATGY 1 cut(s) 1924
BstSCI CCNGG 6 cut(s) 142, 143, 175, 254, 398, 1156
BstSFI CTRYAG 2 cut(s) 875, 1401
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 7 cut(s) 102, 700, 1377, 1450, 1640, 1646, 1648
BstV2I GAAGAC 2 cut(s) 1385, 1490
BstX2I RGATCY 2 cut(s) 384, 562
BstXI CCANNNNNNTGG 2 cut(s) 222, 262
BstYI RGATCY 2 cut(s) 384, 562
Bsu36I CCTNAGG 2 cut(s) 1259, 1878
BsuI GTATCC 1 cut(s) 1566
BsuRI GGCC 3 cut(s) 186, 518, 1876
BtgI CCRYGG 1 cut(s) 1904
BtsCI GGATG 6 cut(s) 158, 510, 684, 1539, 1622, 1703
BtsI GCAGTG 2 cut(s) 663, 1656
BtsIMutI CAGTG 3 cut(s) 663, 1637, 1656
BveI ACCTGC 1 cut(s) 1493
Cac8I GCNNGC 5 cut(s) 510, 1461, 1775, 1779, 1783
CciI TCATGA 1 cut(s) 1611
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 3 cut(s) 126, 246, 1160
Cfr9I CCCGGG 1 cut(s) 143
CpoI CGGWCCG 1 cut(s) 126
CseI GACGC 1 cut(s) 1127
Csp6I GTAC 3 cut(s) 584, 995, 1451
CspAI ACCGGT 1 cut(s) 123
CspI CGGWCCG 1 cut(s) 126
CviAII CATG 8 cut(s) 380, 524, 1276, 1490, 1612, 1678, 1921, 1930
CviQI GTAC 3 cut(s) 584, 995, 1451
DdeI CTNAG 7 cut(s) 58, 63, 552, 1227, 1259, 1409, 1878
DinI GGCGCC 1 cut(s) 213
DpnI GATC 5 cut(s) 386, 564, 1032, 1170, 1528
DpnII GATC 5 cut(s) 384, 562, 1030, 1168, 1526
DriI GACNNNNNGTC 1 cut(s) 1218
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 1311
Eam1105I GACNNNNNGTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1311
EciI GGCGGA 2 cut(s) 82, 241
Ecl136II GAGCTC 1 cut(s) 1408
Eco130I CCWWGG 2 cut(s) 1707, 1871
Eco147I AGGCCT 1 cut(s) 1876
Eco24I GRGCYC 1 cut(s) 1410
Eco32I GATATC 3 cut(s) 802, 1244, 1901
Eco47I GGWCC 3 cut(s) 126, 246, 1160
Eco53kI GAGCTC 1 cut(s) 1408
Eco57I CTGAAG 1 cut(s) 1461
Eco81I CCTNAGG 2 cut(s) 1259, 1878
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 1408
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 3 cut(s) 412, 1798, 1833
EcoRII CCWGG 2 cut(s) 254, 1156
EcoRV GATATC 3 cut(s) 802, 1244, 1901
EcoT14I CCWWGG 2 cut(s) 1707, 1871
EcoT22I ATGCAT 2 cut(s) 529, 1672
EcoT38I GRGCYC 1 cut(s) 1410
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
ErhI CCWWGG 2 cut(s) 1707, 1871
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 8 cut(s) 383, 527, 1279, 1493, 1615, 1681, 1924, 1933
FalI AAGNNNNNCTT 2 cut(s) 1437, 1469
FaqI GGGAC 2 cut(s) 17, 1413
FatI CATG 8 cut(s) 379, 523, 1275, 1489, 1611, 1677, 1920, 1929
FauI CCCGC 3 cut(s) 115, 401, 1366
FokI GGATG 6 cut(s) 145, 517, 691, 1546, 1609, 1690
FriOI GRGCYC 1 cut(s) 1410
FspBI CTAG 2 cut(s) 1247, 1548
GlaI GCGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 593
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 3 cut(s) 186, 518, 1876
HapII CCGG 7 cut(s) 124, 129, 144, 176, 218, 399, 446
HgaI GACGC 1 cut(s) 1127
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 8 cut(s) 383, 527, 1279, 1493, 1615, 1681, 1924, 1933
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HindIII AAGCTT 1 cut(s) 1233
HinfI GANTC 6 cut(s) 395, 651, 787, 990, 1486, 1685
HpaII CCGG 7 cut(s) 124, 129, 144, 176, 218, 399, 446
HphI GGTGA 5 cut(s) 165, 328, 430, 1145, 1322
Hpy166II GTNNAC 4 cut(s) 10, 548, 922, 1508
Hpy188I TCNGA 4 cut(s) 208, 394, 849, 1560
Hpy8I GTNNAC 4 cut(s) 10, 548, 922, 1508
Hpy99I CGWCG 2 cut(s) 430, 1916
HpyAV CCTTC 7 cut(s) 253, 295, 495, 597, 850, 1094, 1412
HpyCH4III ACNGT 3 cut(s) 674, 879, 1908
HpyF10VI GCNNNNNNNGC 7 cut(s) 235, 367, 518, 524, 1232, 1667, 1917
HpyF3I CTNAG 7 cut(s) 58, 63, 552, 1227, 1259, 1409, 1878
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 8 cut(s) 383, 527, 1279, 1493, 1615, 1681, 1924, 1933
HspAI GCGC 1 cut(s) 212
KasI GGCGCC 1 cut(s) 211
Kpn2I TCCGGA 1 cut(s) 445
Kzo9I GATC 5 cut(s) 384, 562, 1030, 1168, 1526
LmnI GCTCC 6 cut(s) 137, 147, 574, 621, 814, 871
Lsp1109I GCAGC 7 cut(s) 102, 700, 1377, 1450, 1640, 1646, 1648
LweI GCATC 5 cut(s) 225, 495, 1024, 1052, 1657
MaeI CTAG 2 cut(s) 1247, 1548
MaeIII GTNAC 5 cut(s) 402, 436, 661, 910, 1567
MalI GATC 5 cut(s) 386, 564, 1032, 1170, 1528
MboI GATC 5 cut(s) 384, 562, 1030, 1168, 1526
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 384, 562
MhlI GDGCHC 1 cut(s) 1410
Mly113I GGCGCC 1 cut(s) 212
MlyI GAGTC 3 cut(s) 796, 999, 1480
MmeI TCCRAC 2 cut(s) 1311, 1891
Mph1103I ATGCAT 2 cut(s) 529, 1672
MroI TCCGGA 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 650
MseI TTAA 4 cut(s) 825, 956, 981, 1433
MslI CAYNNNNRTG 1 cut(s) 1790
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 7 cut(s) 124, 129, 144, 176, 218, 399, 446
MspR9I CCNGG 6 cut(s) 144, 145, 177, 256, 400, 1158
MunI CAATTG 1 cut(s) 897
MvaI CCWGG 2 cut(s) 256, 1158
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 7 cut(s) 235, 367, 518, 524, 1232, 1667, 1917
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 4 cut(s) 144, 145, 177, 400
NdeII GATC 5 cut(s) 384, 562, 1030, 1168, 1526
NlaIII CATG 8 cut(s) 383, 527, 1279, 1493, 1615, 1681, 1924, 1933
NlaIV GGNNCC 7 cut(s) 133, 149, 213, 228, 386, 576, 630
NmuCI GTSAC 4 cut(s) 436, 661, 910, 1567
NsiI ATGCAT 2 cut(s) 529, 1672
NspI RCATGY 1 cut(s) 1924
NspV TTCGAA 2 cut(s) 1723, 1837
PagI TCATGA 1 cut(s) 1611
PceI AGGCCT 1 cut(s) 1876
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 3 cut(s) 395, 651, 1685
PflFI GACNNNGTC 1 cut(s) 1137
PflMI CCANNNNNTGG 1 cut(s) 529
PinAI ACCGGT 1 cut(s) 123
PleI GAGTC 3 cut(s) 795, 998, 1480
PluTI GGCGCC 1 cut(s) 215
PpsI GAGTC 3 cut(s) 795, 998, 1480
PpuMI RGGWCCY 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 1410
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 254, 1156
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 2 cut(s) 254, 1156
PspN4I GGNNCC 7 cut(s) 133, 149, 213, 228, 386, 576, 630
PspPI GGNCC 3 cut(s) 126, 246, 1160
PspPPI RGGWCCY 1 cut(s) 246
PsrI GAACNNNNNNTAC 1 cut(s) 32
PstI CTGCAG 1 cut(s) 1405
PsuI RGATCY 2 cut(s) 384, 562
PsyI GACNNNGTC 1 cut(s) 1137
RsaI GTAC 3 cut(s) 585, 996, 1452
RsaNI GTAC 3 cut(s) 584, 995, 1451
RseI CAYNNNNRTG 1 cut(s) 1790
Rsr2I CGGWCCG 1 cut(s) 126
RsrII CGGWCCG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 1410
SaqAI TTAA 4 cut(s) 825, 956, 981, 1433
Sau3AI GATC 5 cut(s) 384, 562, 1030, 1168, 1526
Sau96I GGNCC 3 cut(s) 126, 246, 1160
ScaI AGTACT 1 cut(s) 1452
SchI GAGTC 3 cut(s) 796, 999, 1480
ScrFI CCNGG 6 cut(s) 144, 145, 177, 256, 400, 1158
SduI GDGCHC 1 cut(s) 1410
SfaNI GCATC 5 cut(s) 225, 495, 1024, 1052, 1657
SfcI CTRYAG 2 cut(s) 875, 1401
SfoI GGCGCC 1 cut(s) 213
SfuI TTCGAA 2 cut(s) 1723, 1837
SinI GGWCC 3 cut(s) 126, 246, 1160
SmaI CCCGGG 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 1790
SmlI CTYRAG 2 cut(s) 980, 1373
SmoI CTYRAG 2 cut(s) 980, 1373
SseBI AGGCCT 1 cut(s) 1876
SsiI CCGC 8 cut(s) 93, 108, 151, 252, 367, 408, 519, 1359
SspDI GGCGCC 1 cut(s) 211
SspMI CTAG 2 cut(s) 1247, 1548
SstI GAGCTC 1 cut(s) 1410
StuI AGGCCT 1 cut(s) 1876
StyD4I CCNGG 6 cut(s) 142, 143, 175, 254, 398, 1156
StyI CCWWGG 2 cut(s) 1707, 1871
TaaI ACNGT 3 cut(s) 674, 879, 1908
TaqI TCGA 5 cut(s) 72, 1060, 1723, 1809, 1837
TaqII GACCGA 1 cut(s) 1697
TatI WGTACW 1 cut(s) 1450
TauI GCSGC 3 cut(s) 153, 370, 521
TfiI GAWTC 3 cut(s) 395, 651, 1685
Tru1I TTAA 4 cut(s) 825, 956, 981, 1433
Tru9I TTAA 4 cut(s) 825, 956, 981, 1433
TscAI CASTG 3 cut(s) 670, 1644, 1663
TseFI GTSAC 4 cut(s) 436, 661, 910, 1567
TseI GCWGC 7 cut(s) 115, 688, 1390, 1463, 1634, 1653, 1661
Tsp45I GTSAC 4 cut(s) 436, 661, 910, 1567
TspGWI ACGGA 3 cut(s) 377, 424, 634
TspMI CCCGGG 1 cut(s) 143
TspRI CASTG 3 cut(s) 670, 1644, 1663
Tth111I GACNNNGTC 1 cut(s) 1137
Van91I CCANNNNNTGG 1 cut(s) 529
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 3 cut(s) 126, 246, 1160
VspI ATTAAT 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 5 cut(s) 412, 1579, 1615, 1798, 1833
XceI RCATGY 1 cut(s) 1924
XmaI CCCGGG 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 2 cut(s) 1247, 1548
ZrmI AGTACT 1 cut(s) 1452
Zsp2I ATGCAT 2 cut(s) 529, 1672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.