Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003352

Physical Location & Seq

Genomic Coordinates Forward (+)

47386800 .. 47390796

Gene Structure 3997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1358600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGGATCATCATCCAGTCTCCGACGACCCCAAAACTCCACTTCTCCCTCTCCACGATAAAATCCAACGGTCGCAGAGCTTCCATTCATCCTTGAAATCCCTTCTAACACTCAAGAACTTCTTCGTCCTCCTGGGACCTCTTCTGTGCACCATCATATGCCTCTGTGTGAAGCTAGATGGCCCGGTGGCCAGCCGGAACATGTTGGCGGTGCTGGTGTGGGTGTTTGCTTGGTGGGTCACGGAGGCCGTGCCCATGCCAATTACCTCCATGTCCCCACTCTTTCTCTTCCCTCTCTTTGGAATATCCTCTGCTGATGATGTTGCTCAATCTTACATGGATGATGTTATTGCCCTTGTTCTTGGAAGCTTTATATTGGCTCTTGCTGTTGAGCATTATAACATCCACAGAAGATTGGCCTTGAATATAACCATGCTCTTCTGCGGAGACCCGCTGAGTCCTCCCCTGCTCCTACTGGGAATATGCGCCACGACAGCATTCGTCTCCATGTGGATGCACAACGTGGCGGCAGCTGTGATAATGATGCCGGTGGCCACTGGGATCCTCCAGCGCTTTCCGGTGTGTCCGGACCAGTCCGTTGCCGAGAGCAAGTTCTGCAGGGCGGTGGTGCTGGGGGTGATATACTCCGCGGCCATAGGAGGGATGAGCACTCTCACTGGGACAGGTGTGAACTTGATATTGGTTGGGATGTGGAAGAGCTATTTTCCAGAGGCGAAGCCCATCAGCTTCAGCACCTGGTTCTTTCTAGGGTTCCCTCTGGCCTTGTTGATATTCTTGGCTTTGTGGGTTATACTTTGTTGCTTGTATTGCCCAAGGAATTCAGGCCAGGCTCTCTCTGCTTATCTGGACAAAGCTCACTTGAAGAGGGAGCTTGAAATGCTAGGTCCAATGGCTTTTGCTGAGAAGATGGTACTGGCTGTGTTTTCGATGCTGATAGTTTTGTGGATGACAAGGAGCATAACAGATGATATTCCTGGGTGGGGATCCCTCTTCAATGGGCGTGCTGGTGATGGAACTGTCAGTGTTATGATGGCAACTTTGCTGTTCATAATTCCAAACAAAAAGCAAGAGGGAGAGAAGTTGATGGACTGGAACAAATGCAAGAAGCTACCATGGAACATCATATTGTTACTAGGTGCTGGTTTTGCCATCGCCGACGGAGTCCGAACTAGCGGCCTTGCAGACATTTTATCTAAAGCCCTAGATTTCTTGGAGGCTGTCCCATATATGGCCATTGCGCCGGCTGTGTGTCTCATAAGTAGTACCATCACTGAGTTCACATCAAACAATGCCACCACCACTCTTGTTGTCCCTCTTTTGATTCAAATAGCCAAAAGCATGCATGTACATCCACTCCTCCTTATGATTCCAGGAGCCATTGGGGCACAGTTTTCTTTCTTGCTTCCGACCGGTACCCCTTCGAATATTGTAGGGTTTACCACTGGACACATTGAAATTCAAGACATGATTAAGACTGGATTGCCACTGAAGATTGCTGGAACTGTTGTGCTGTCCCTTCTGATGCCCACACTAGGAGCTTATGTATTTGGGACAAATGAACCTGCAGTTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0006873 GO:0006885 GO:0008150 GO:0008509 GO:0008514 GO:0009987 GO:0015075 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0016021 GO:0019725 GO:0022857 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0034220 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046942 GO:0046943 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071423 GO:0071702 GO:0098656 GO:0098771 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_sulph_symp

Protein Analysis

531

Amino Acids

57.58

Weight (kDa)

6.82

Isoelectric Point (pI)

44.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_sulph_symp	PF00939	61 - 514	6.1e-59	Sodium:sulfate symporter transmembrane region
CitMHS	PF03600	75 - 461	1.7e-45	Citrate transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47560
fragaria_vesca	FvH4_3g00890 FvH4_3g00891 FvH4_3g00891
malus_domestica	MD05G1358600.v1.1 MD10G1332900.v1.1 MD10G1333000.v1.1
prunus_persica	Prupe.4G009400_v2.0.a1 Prupe.4G009500_v2.0.a1 Prupe.4G009500_v2.0.a1
pyrus_communis	pycom05g32430 pycom10g28220 pycom10g28230 pycom10g28300 pycom10g28330
rosa_chinensis	RchiOBHm_Chr5g0001361 RchiOBHm_Chr5g0001371
rosa_laevigata	RLG00000030922 RLG00000030923
rosa_multiflora	Rmu_co8451069.1_g000001 Rmu_sc0001706.1_g000004 Rmu_sc0003749.1_g000019 Rmu_sc0003900.1_g000003
rosa_roxburghii	Rroxscaffold_1G00074990
rosa_rugosa	Rorug04G0390100.1 Rorug04G0390200
rosa_samantha	Rh5AG010400 Rh5AG010500 Rh5BG013100 Rh5BG013300 Rh5CG011400 Rh5CG011500 Rh5DG010800 Rh5DG010900
rosa_wichuraiana	Rw5G001000 Rw5G001010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	396
Acc36I	ACCTGC	1 cut(s)	1588
Acc65I	GGTACC	1 cut(s)	1430
AccB1I	GGYRCC	1 cut(s)	1430
AccII	CGCG	1 cut(s)	647
AccIII	TCCGGA	1 cut(s)	583
AciI	CCGC	8 cut(s)	206, 441, 449, 524, 620, 645, 647, 1191
AclWI	GGATC	5 cut(s)	12, 553, 566, 996, 1009
AcoI	YGGCCR	4 cut(s)	186, 549, 648, 1248
AcsI	RAATTY	2 cut(s)	835, 1473
AcuI	CTGAAG	2 cut(s)	730, 1526
AdeI	CACNNNGTG	1 cut(s)	520
AfaI	GTAC	4 cut(s)	930, 1282, 1365, 1432
AfeI	AGCGCT	1 cut(s)	569
AfiI	CCNNNNNNNGG	5 cut(s)	296, 657, 998, 1246, 1550
AflIII	ACRYGT	1 cut(s)	198
AgeI	ACCGGT	1 cut(s)	1427
AgsI	TTSAA	9 cut(s)	94, 421, 880, 893, 1012, 1343, 1472, 1478, 1589
AjnI	CCWGG	5 cut(s)	129, 752, 843, 991, 1387
AjuI	GAANNNNNNNTTGG	2 cut(s)	680, 712
Alw21I	GWGCWC	2 cut(s)	149, 668
Alw26I	GTCTC	4 cut(s)	22, 438, 505, 1274
Alw44I	GTGCAC	1 cut(s)	145
AlwI	GGATC	5 cut(s)	12, 553, 566, 996, 1009
AlwNI	CAGNNNCTG	1 cut(s)	753
Aor13HI	TCCGGA	1 cut(s)	583
Aor51HI	AGCGCT	1 cut(s)	569
ApaLI	GTGCAC	1 cut(s)	145
ApeKI	GCWGC	1 cut(s)	527
ApoI	RAATTY	2 cut(s)	835, 1473
AsiGI	ACCGGT	1 cut(s)	1427
Asp700I	GAANNNNTTC	1 cut(s)	119
Asp718I	GGTACC	1 cut(s)	1430
AspLEI	GCGC	3 cut(s)	485, 570, 1258
AspS9I	GGNCC	4 cut(s)	134, 179, 586, 902
AsuC2I	CCSGG	1 cut(s)	182
AsuHPI	GGTGA	2 cut(s)	646, 1037
AsuII	TTCGAA	1 cut(s)	1439
AvaII	GGWCC	3 cut(s)	134, 586, 902
BaeGI	GKGCMC	3 cut(s)	149, 252, 1405
BalI	TGGCCA	3 cut(s)	188, 551, 1250
BamHI	GGATCC	2 cut(s)	558, 1001
BanI	GGYRCC	1 cut(s)	1430
Bbv12I	GWGCWC	2 cut(s)	149, 668
BbvI	GCAGC	1 cut(s)	539
BccI	CCATC	9 cut(s)	158, 170, 746, 919, 1022, 1042, 1096, 1175, 1292
BceAI	ACGGC	1 cut(s)	230
BciT130I	CCWGG	5 cut(s)	131, 754, 845, 993, 1389
BcnI	CCSGG	1 cut(s)	182
BcoDI	GTCTC	4 cut(s)	22, 438, 505, 1274
BfaI	CTAG	7 cut(s)	173, 764, 899, 1151, 1188, 1220, 1550
BfmI	CTRYAG	2 cut(s)	613, 1581
BfoI	RGCGCY	1 cut(s)	571
BfuAI	ACCTGC	1 cut(s)	1588
BglI	GCCNNNNNGGC	1 cut(s)	1400
BisI	GCNGC	4 cut(s)	525, 528, 648, 1192
BlsI	GCNGC	4 cut(s)	526, 529, 649, 1193
Bme1390I	CCNGG	6 cut(s)	131, 182, 754, 845, 993, 1389
Bme18I	GGWCC	3 cut(s)	134, 586, 902
BmgT120I	GGNCC	4 cut(s)	134, 179, 586, 902
BmiI	GGNNCC	6 cut(s)	135, 560, 770, 1003, 1393, 1432
BmrFI	CCNGG	6 cut(s)	131, 182, 754, 845, 993, 1389
BmrI	ACTGGG	3 cut(s)	482, 564, 684
BmsI	GCATC	4 cut(s)	501, 531, 936, 1530
BmuI	ACTGGG	3 cut(s)	482, 564, 684
BpmI	CTGGAG	1 cut(s)	548
Bpu14I	TTCGAA	1 cut(s)	1439
BpuEI	CTTGAG	1 cut(s)	95
BpuMI	CCSGG	1 cut(s)	182
BsaBI	GATNNNNATC	1 cut(s)	9
BsaI	GGTCTC	1 cut(s)	438
BsaJI	CCNNGG	5 cut(s)	130, 645, 830, 992, 1130
BsaWI	WCCGGW	3 cut(s)	574, 583, 1427
BsaXI	ACNNNNNCTCC	4 cut(s)	1356, 1386, 1545, 1575
Bsc4I	CCNNNNNNNGG	5 cut(s)	296, 657, 998, 1246, 1550
Bse118I	RCCGGY	3 cut(s)	544, 1258, 1427
Bse1I	ACTGG	9 cut(s)	14, 477, 559, 589, 679, 936, 1112, 1465, 1498
Bse3DI	GCAATG	1 cut(s)	1251
Bse8I	GATNNNNATC	1 cut(s)	9
BseAI	TCCGGA	1 cut(s)	583
BseBI	CCWGG	5 cut(s)	131, 754, 845, 993, 1389
BseDI	CCNNGG	5 cut(s)	130, 645, 830, 992, 1130
BseGI	GGATG	9 cut(s)	10, 86, 343, 399, 516, 666, 711, 969, 1366
BseJI	GATNNNNATC	1 cut(s)	9
BseLI	CCNNNNNNNGG	5 cut(s)	296, 657, 998, 1246, 1550
BseMI	GCAATG	1 cut(s)	1251
BseMII	CTCAG	3 cut(s)	443, 909, 1281
BseNI	ACTGG	9 cut(s)	14, 477, 559, 589, 679, 936, 1112, 1465, 1498
BseRI	GAGGAG	1 cut(s)	1364
BseSI	GKGCMC	3 cut(s)	149, 252, 1405
BseXI	GCAGC	1 cut(s)	539
BseYI	CCCAGC	1 cut(s)	628
Bsh1236I	CGCG	1 cut(s)	647
Bsh1285I	CGRYCG	2 cut(s)	71, 1428
BshNI	GGYRCC	1 cut(s)	1430
BshTI	ACCGGT	1 cut(s)	1427
BsiEI	CGRYCG	2 cut(s)	71, 1428
BsiHKAI	GWGCWC	2 cut(s)	149, 668
BsiSI	CCGG	7 cut(s)	182, 193, 545, 575, 584, 1259, 1428
BslFI	GGGAC	7 cut(s)	147, 256, 691, 1223, 1313, 1516, 1582
BslI	CCNNNNNNNGG	5 cut(s)	296, 657, 998, 1246, 1550
BsmAI	GTCTC	4 cut(s)	22, 438, 505, 1274
BsmBI	CGTCTC	1 cut(s)	505
BsmFI	GGGAC	7 cut(s)	147, 256, 691, 1223, 1313, 1516, 1582
BsmI	GAATGC	1 cut(s)	494
Bso31I	GGTCTC	1 cut(s)	438
Bsp119I	TTCGAA	1 cut(s)	1439
Bsp1286I	GDGCHC	4 cut(s)	149, 252, 668, 1405
Bsp13I	TCCGGA	1 cut(s)	583
Bsp1407I	TGTACA	1 cut(s)	1363
Bsp143I	GATC	3 cut(s)	4, 558, 1001
Bsp19I	CCATGG	1 cut(s)	1130
BspACI	CCGC	8 cut(s)	206, 441, 449, 524, 620, 645, 647, 1191
BspCNI	CTCAG	3 cut(s)	444, 910, 1282
BspEI	TCCGGA	1 cut(s)	583
BspFNI	CGCG	1 cut(s)	647
BspLI	GGNNCC	6 cut(s)	135, 560, 770, 1003, 1393, 1432
BspMAI	CTGCAG	2 cut(s)	617, 1585
BspMI	ACCTGC	1 cut(s)	1588
BspPI	GGATC	5 cut(s)	12, 553, 566, 996, 1009
BspQI	GCTCTTC	2 cut(s)	440, 707
BspT104I	TTCGAA	1 cut(s)	1439
BspT107I	GGYRCC	1 cut(s)	1430
BspTNI	GGTCTC	1 cut(s)	438
BsrDI	GCAATG	1 cut(s)	1251
BsrFI	RCCGGY	3 cut(s)	544, 1258, 1427
BsrGI	TGTACA	1 cut(s)	1363
BsrI	ACTGG	9 cut(s)	14, 477, 559, 589, 679, 936, 1112, 1465, 1498
BssAI	RCCGGY	3 cut(s)	544, 1258, 1427
BssECI	CCNNGG	5 cut(s)	130, 645, 830, 992, 1130
BssMI	GATC	3 cut(s)	4, 558, 1001
BssT1I	CCWWGG	2 cut(s)	830, 1130
Bst2UI	CCWGG	5 cut(s)	131, 754, 845, 993, 1389
Bst4CI	ACNGT	4 cut(s)	69, 1036, 1407, 1522
Bst6I	CTCTTC	6 cut(s)	144, 290, 440, 707, 875, 1013
BstAPI	GCANNNNNTGC	1 cut(s)	612
BstAUI	TGTACA	1 cut(s)	1363
BstBI	TTCGAA	1 cut(s)	1439
BstC8I	GCNNGC	4 cut(s)	190, 1020, 1260, 1358
BstDEI	CTNAG	3 cut(s)	452, 918, 1290
BstDSI	CCRYGG	2 cut(s)	645, 1130
BstF5I	GGATG	9 cut(s)	10, 86, 343, 399, 516, 666, 711, 969, 1366
BstFNI	CGCG	1 cut(s)	647
BstH2I	RGCGCY	1 cut(s)	571
BstHHI	GCGC	3 cut(s)	485, 570, 1258
BstKTI	GATC	3 cut(s)	7, 561, 1004
BstMAI	GTCTC	4 cut(s)	22, 438, 505, 1274
BstMBI	GATC	3 cut(s)	4, 558, 1001
BstMCI	CGRYCG	2 cut(s)	71, 1428
BstMWI	GCNNNNNNNGC	7 cut(s)	491, 612, 825, 854, 895, 1163, 1400
BstNI	CCWGG	5 cut(s)	131, 754, 845, 993, 1389
BstNSI	RCATGY	3 cut(s)	202, 1360, 1364
BstSCI	CCNGG	6 cut(s)	129, 180, 752, 843, 991, 1387
BstSFI	CTRYAG	2 cut(s)	613, 1581
BstSLI	GKGCMC	3 cut(s)	149, 252, 1405
BstUI	CGCG	1 cut(s)	647
BstV1I	GCAGC	1 cut(s)	539
BstX2I	RGATCY	2 cut(s)	558, 1001
BstYI	RGATCY	2 cut(s)	558, 1001
BtgI	CCRYGG	2 cut(s)	645, 1130
BtgZI	GCGATG	1 cut(s)	1153
BtsCI	GGATG	9 cut(s)	10, 86, 343, 399, 516, 666, 711, 969, 1366
BtsIMutI	CAGTG	6 cut(s)	552, 672, 1045, 1287, 1458, 1502
BveI	ACCTGC	1 cut(s)	1588
Cac8I	GCNNGC	4 cut(s)	190, 1020, 1260, 1358
CaiI	CAGNNNCTG	1 cut(s)	753
CfoI	GCGC	3 cut(s)	485, 570, 1258
Cfr10I	RCCGGY	3 cut(s)	544, 1258, 1427
Cfr13I	GGNCC	4 cut(s)	134, 179, 586, 902
Cfr42I	CCGCGG	1 cut(s)	648
CsiI	ACCWGGT	1 cut(s)	752
Csp6I	GTAC	4 cut(s)	929, 1281, 1364, 1431
CspAI	ACCGGT	1 cut(s)	1427
CspCI	CAANNNNNGTGG	4 cut(s)	1303, 1306, 1338, 1341
CviQI	GTAC	4 cut(s)	929, 1281, 1364, 1431
DdeI	CTNAG	3 cut(s)	452, 918, 1290
DpnI	GATC	3 cut(s)	6, 560, 1003
DpnII	GATC	3 cut(s)	4, 558, 1001
DraIII	CACNNNGTG	1 cut(s)	520
EaeI	YGGCCR	4 cut(s)	186, 549, 648, 1248
Eam1104I	CTCTTC	6 cut(s)	144, 290, 440, 707, 875, 1013
EarI	CTCTTC	6 cut(s)	144, 290, 440, 707, 875, 1013
Eco130I	CCWWGG	2 cut(s)	830, 1130
Eco31I	GGTCTC	1 cut(s)	438
Eco47I	GGWCC	3 cut(s)	134, 586, 902
Eco47III	AGCGCT	1 cut(s)	569
Eco57I	CTGAAG	2 cut(s)	730, 1526
EcoO109I	RGGNCCY	1 cut(s)	134
EcoRI	GAATTC	1 cut(s)	835
EcoRII	CCWGG	5 cut(s)	129, 752, 843, 991, 1387
EcoT14I	CCWWGG	2 cut(s)	830, 1130
EcoT22I	ATGCAT	1 cut(s)	1362
ErhI	CCWWGG	2 cut(s)	830, 1130
Esp3I	CGTCTC	1 cut(s)	505
FalI	AAGNNNNNCTT	2 cut(s)	104, 136
FaqI	GGGAC	7 cut(s)	147, 256, 691, 1223, 1313, 1516, 1582
FauI	CCCGC	1 cut(s)	456
FauNDI	CATATG	1 cut(s)	155
Fnu4HI	GCNGC	4 cut(s)	525, 528, 648, 1192
FokI	GGATG	8 cut(s)	73, 350, 386, 523, 673, 718, 976, 1353
Fsp4HI	GCNGC	4 cut(s)	525, 528, 648, 1192
FspBI	CTAG	7 cut(s)	173, 764, 899, 1151, 1188, 1220, 1550
GlaI	GCGC	3 cut(s)	484, 569, 1257
GluI	GCNGC	4 cut(s)	525, 528, 648, 1192
GsaI	CCCAGC	1 cut(s)	632
GsuI	CTGGAG	1 cut(s)	548
HaeII	RGCGCY	1 cut(s)	571
HapII	CCGG	7 cut(s)	182, 193, 545, 575, 584, 1259, 1428
HhaI	GCGC	3 cut(s)	485, 570, 1258
Hin6I	GCGC	3 cut(s)	483, 568, 1256
HinP1I	GCGC	3 cut(s)	483, 568, 1256
HindIII	AAGCTT	1 cut(s)	364
HinfI	GANTC	4 cut(s)	454, 1179, 1339, 1384
HpaII	CCGG	7 cut(s)	182, 193, 545, 575, 584, 1259, 1428
HphI	GGTGA	2 cut(s)	646, 1037
Hpy166II	GTNNAC	4 cut(s)	147, 688, 1296, 1455
Hpy188I	TCNGA	4 cut(s)	22, 1184, 1425, 1539
Hpy188III	TCNNGA	5 cut(s)	112, 584, 725, 863, 1478
Hpy8I	GTNNAC	4 cut(s)	147, 688, 1296, 1455
Hpy99I	CGWCG	2 cut(s)	26, 1178
HpyAV	CCTTC	3 cut(s)	110, 1446, 1544
HpyCH4III	ACNGT	4 cut(s)	69, 1036, 1407, 1522
HpyCH4IV	ACGT	1 cut(s)	519
HpyCH4V	TGCA	7 cut(s)	147, 514, 615, 1119, 1199, 1360, 1583
HpyF10VI	GCNNNNNNNGC	7 cut(s)	491, 612, 825, 854, 895, 1163, 1400
HpyF3I	CTNAG	3 cut(s)	452, 918, 1290
HpySE526I	ACGT	1 cut(s)	519
HspAI	GCGC	3 cut(s)	483, 568, 1256
Kpn2I	TCCGGA	1 cut(s)	583
KpnI	GGTACC	1 cut(s)	1434
KroI	GCCGGC	1 cut(s)	1258
KroNI	GCCGGC	1 cut(s)	1260
KspI	CCGCGG	1 cut(s)	648
Kzo9I	GATC	3 cut(s)	4, 558, 1001
LguI	GCTCTTC	2 cut(s)	440, 707
LmnI	GCTCC	5 cut(s)	471, 886, 972, 1391, 1553
Lsp1109I	GCAGC	1 cut(s)	539
LweI	GCATC	4 cut(s)	501, 531, 936, 1530
MabI	ACCWGGT	1 cut(s)	752
MaeI	CTAG	7 cut(s)	173, 764, 899, 1151, 1188, 1220, 1550
MaeII	ACGT	1 cut(s)	519
MaeIII	GTNAC	2 cut(s)	235, 1146
MalI	GATC	3 cut(s)	6, 560, 1003
MboI	GATC	3 cut(s)	4, 558, 1001
MflI	RGATCY	2 cut(s)	558, 1001
MhlI	GDGCHC	4 cut(s)	149, 252, 668, 1405
MlsI	TGGCCA	3 cut(s)	188, 551, 1250
MluCI	AATT	4 cut(s)	258, 835, 1068, 1473
MluNI	TGGCCA	3 cut(s)	188, 551, 1250
MlyI	GAGTC	2 cut(s)	463, 1188
MmeI	TCCRAC	3 cut(s)	45, 88, 1448
Mox20I	TGGCCA	3 cut(s)	188, 551, 1250
Mph1103I	ATGCAT	1 cut(s)	1362
MroI	TCCGGA	1 cut(s)	583
MroNI	GCCGGC	1 cut(s)	1258
MroXI	GAANNNNTTC	1 cut(s)	119
MscI	TGGCCA	3 cut(s)	188, 551, 1250
MseI	TTAA	1 cut(s)	1488
MslI	CAYNNNNRTG	1 cut(s)	509
Msp20I	TGGCCA	3 cut(s)	188, 551, 1250
MspA1I	CMGCKG	3 cut(s)	451, 530, 647
MspI	CCGG	7 cut(s)	182, 193, 545, 575, 584, 1259, 1428
MspR9I	CCNGG	6 cut(s)	131, 182, 754, 845, 993, 1389
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	5 cut(s)	131, 754, 845, 993, 1389
MvnI	CGCG	1 cut(s)	647
MwoI	GCNNNNNNNGC	7 cut(s)	491, 612, 825, 854, 895, 1163, 1400
NaeI	GCCGGC	1 cut(s)	1260
NciI	CCSGG	1 cut(s)	182
NcoI	CCATGG	1 cut(s)	1130
NdeI	CATATG	1 cut(s)	155
NdeII	GATC	3 cut(s)	4, 558, 1001
NgoMIV	GCCGGC	1 cut(s)	1258
NlaIV	GGNNCC	6 cut(s)	135, 560, 770, 1003, 1393, 1432
NmeAIII	GCCGAG	1 cut(s)	625
NmuCI	GTSAC	1 cut(s)	235
NsiI	ATGCAT	1 cut(s)	1362
NspI	RCATGY	3 cut(s)	202, 1360, 1364
NspV	TTCGAA	1 cut(s)	1439
PaeI	GCATGC	1 cut(s)	1360
PciI	ACATGT	1 cut(s)	198
PciSI	GCTCTTC	2 cut(s)	440, 707
PctI	GAATGC	1 cut(s)	494
PdiI	GCCGGC	1 cut(s)	1260
PdmI	GAANNNNTTC	1 cut(s)	119
PfeI	GAWTC	2 cut(s)	1339, 1384
PflFI	GACNNNGTC	1 cut(s)	1178
PfoI	TCCNGGA	1 cut(s)	1387
PinAI	ACCGGT	1 cut(s)	1427
PkrI	GCNGC	4 cut(s)	526, 529, 649, 1193
PleI	GAGTC	2 cut(s)	462, 1187
PpsI	GAGTC	2 cut(s)	462, 1187
PpuMI	RGGWCCY	1 cut(s)	134
PscI	ACATGT	1 cut(s)	198
PsiI	TTATAA	1 cut(s)	396
Psp5II	RGGWCCY	1 cut(s)	134
Psp6I	CCWGG	5 cut(s)	129, 752, 843, 991, 1387
PspFI	CCCAGC	1 cut(s)	628
PspGI	CCWGG	5 cut(s)	129, 752, 843, 991, 1387
PspN4I	GGNNCC	6 cut(s)	135, 560, 770, 1003, 1393, 1432
PspPI	GGNCC	4 cut(s)	134, 179, 586, 902
PspPPI	RGGWCCY	1 cut(s)	134
PstI	CTGCAG	2 cut(s)	617, 1585
PstNI	CAGNNNCTG	1 cut(s)	753
PsuI	RGATCY	2 cut(s)	558, 1001
PsyI	GACNNNGTC	1 cut(s)	1178
PvuII	CAGCTG	1 cut(s)	530
RsaI	GTAC	4 cut(s)	930, 1282, 1365, 1432
RsaNI	GTAC	4 cut(s)	929, 1281, 1364, 1431
RseI	CAYNNNNRTG	1 cut(s)	509
SacII	CCGCGG	1 cut(s)	648
SapI	GCTCTTC	2 cut(s)	440, 707
SaqAI	TTAA	1 cut(s)	1488
SatI	GCNGC	4 cut(s)	525, 528, 648, 1192
Sau3AI	GATC	3 cut(s)	4, 558, 1001
Sau96I	GGNCC	4 cut(s)	134, 179, 586, 902
SchI	GAGTC	2 cut(s)	463, 1188
ScrFI	CCNGG	6 cut(s)	131, 182, 754, 845, 993, 1389
SduI	GDGCHC	4 cut(s)	149, 252, 668, 1405
SexAI	ACCWGGT	1 cut(s)	752
SfaNI	GCATC	4 cut(s)	501, 531, 936, 1530
SfcI	CTRYAG	2 cut(s)	613, 1581
Sfr303I	CCGCGG	1 cut(s)	648
SfuI	TTCGAA	1 cut(s)	1439
SgrBI	CCGCGG	1 cut(s)	648
SinI	GGWCC	3 cut(s)	134, 586, 902
SmiMI	CAYNNNNRTG	1 cut(s)	509
SmlI	CTYRAG	1 cut(s)	110
SmoI	CTYRAG	1 cut(s)	110
SphI	GCATGC	1 cut(s)	1360
Sse9I	AATT	4 cut(s)	258, 835, 1068, 1473
SsiI	CCGC	8 cut(s)	206, 441, 449, 524, 620, 645, 647, 1191
SspI	AATATT	1 cut(s)	1444
SspMI	CTAG	7 cut(s)	173, 764, 899, 1151, 1188, 1220, 1550
StyD4I	CCNGG	6 cut(s)	129, 180, 752, 843, 991, 1387
StyI	CCWWGG	2 cut(s)	830, 1130
TaaI	ACNGT	4 cut(s)	69, 1036, 1407, 1522
TaiI	ACGT	1 cut(s)	522
TaqI	TCGA	2 cut(s)	944, 1439
TasI	AATT	4 cut(s)	258, 835, 1068, 1473
TatI	WGTACW	1 cut(s)	1363
TauI	GCSGC	3 cut(s)	527, 650, 1194
TfiI	GAWTC	2 cut(s)	1339, 1384
Tru1I	TTAA	1 cut(s)	1488
Tru9I	TTAA	1 cut(s)	1488
TscAI	CASTG	6 cut(s)	559, 679, 1045, 1294, 1465, 1509
TseFI	GTSAC	1 cut(s)	235
TseI	GCWGC	1 cut(s)	527
Tsp45I	GTSAC	1 cut(s)	235
TspDTI	ATGAA	3 cut(s)	75, 1054, 1590
TspGWI	ACGGA	3 cut(s)	254, 583, 1191
TspRI	CASTG	6 cut(s)	559, 679, 1045, 1294, 1465, 1509
Tth111I	GACNNNGTC	1 cut(s)	1178
VneI	GTGCAC	1 cut(s)	145
VpaK11BI	GGWCC	3 cut(s)	134, 586, 902
XapI	RAATTY	2 cut(s)	835, 1473
XceI	RCATGY	3 cut(s)	202, 1360, 1364
XmnI	GAANNNNTTC	1 cut(s)	119
XspI	CTAG	7 cut(s)	173, 764, 899, 1151, 1188, 1220, 1550
Zsp2I	ATGCAT	1 cut(s)	1362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.