Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003352

Physical Location & Seq

Genomic Coordinates Forward (+)

41023257 .. 41028994

Gene Structure 5738 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1333000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGATAATCATCCAATATCCGAAGACCCCAAAACTCCACTTCTCCCTCTCCACGATCAAATCCAACGGTCGCAGAGCTTCCATTCATCCTTGAAATCAATTCTAACACTCAAGAACTTCTTCGTCCTCCTCGGACCTCTTCTGTGCACCATTATATGCCTCTGTGTGAAGCTAGATGGCCCGGTGGCCAGCCGGAACATGTTGGCGGTGCTGGTGTGGGTGTTTGCTTGGTGGCTCACGGAGGCCGTGCCCATGCCAATTACCTCCATGTCCCCACTCTTTCTCTTCCCTCTCTTCGGAATTGCCTCTGCAGATGATGTTGCCCACTCTTACATGGATGATGTTATTGCCCTTCTTCTTGGAAGCTTTATATTGGCTCTTGCTGTTGAGCATTACAACATCCACAGAAGATTGGCCTTGAATATAACCCTGGTGTTCTGCGGAGATCCCTTGAATCCGCCATTGCTGCTGCTGGGAATATGTGGGACCGCAGCATTCATCAGCATGTGGATGCACAACGTGGCAACTGCGGTGATGATGATGCCCGTAGCCACCGGCATTCTCCGGCGGTTTCCAACGGGTCCCGACCAGTCCGTGGTTGCGAGCAACTTTTGCAAAGCCGTGATTCTGGGGATGTTGTACTCCATAACCATAGGAGGGATGAGCACTCTGACGGGGACAGGTGTCAATCTCATATTGGTTGGAATGTGGAAGAGCTATTTCCCACAAGCAGCTCCGATCACCTTCAGCACCTGGTTCTGTTTCGCCTTCCCTTCGGCATTGTTGATGTTCTTGGCGCTGTGGGCTTTGCTTTGCTACTTGTATTGCTCAAGGAGTTCAGGCCAGGCTCTCTCTGTTTATTTAGACAAAGCCCATTTGAAGAAGGAGCTCGAATTGCTAGGTCCAATGGCTTTTGCTGAGAAGATGGTACTGGCTTTGTTTTCGATGCTGATTGTTTTGTGGATGACAAGAAGCATAACGGAGGATATTCCGGGCTGGGGAGTCCTCTTCAACGGGCGTGCCGGAGATGGAACTGTCAGTGTTATGGTTGCAACATTTTTGTTCATATTTCCAAGCAAAAAGGAAAAGGGTGAGAAGTTGATGGACTGGGAAAAATGCAAGAAGCTACCCTGGAATATCATATTGTTACTAGGTGCTGGTTTAGCCATAGCTGACGGAGTTCGATCCAGTGGCCTTGCTAATATATTATCTGAAACCCTAGATTTTTTGGAGGCAGTCCCATATGTTGCCATTGCACCTGCTGTGTGTCTCATAAGTAGTTCCATCACTGAGTTGATCACATCAAACAATGCGACAACCACGCTTATTATTCCTCTCCTAATTCAAATTGCCAGAAATATGCATGTGCATCCTCTCCTTCTGATGATTCCGGGTGGCATTGGAGCACAATTTGCTTTCTTGCTTCCTACGGCAACTCCTTCAAATACCGTAGGATTTGCCACTGGCCGCATTGAAATCCAAGATATGATCAAGATTGGCTTGCCATTGAAGATTGTTGGGATTGCTGTGTTGTCCCTTTTGATGCCCACTCTCGGAGCTTATGTATTTAAGACAAGTGAACCAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0006873 GO:0006885 GO:0008150 GO:0008509 GO:0008514 GO:0009987 GO:0015075 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0016021 GO:0019725 GO:0022857 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0034220 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046942 GO:0046943 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071423 GO:0071702 GO:0098656 GO:0098771 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_sulph_symp

Protein Analysis

530

Amino Acids

57.57

Weight (kDa)

7.11

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_sulph_symp	PF00939	61 - 516	4e-56	Sodium:sulfate symporter transmembrane region
CitMHS	PF03600	75 - 463	3.3e-41	Citrate transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47560
fragaria_vesca	FvH4_3g00890 FvH4_3g00891 FvH4_3g00891
malus_domestica	MD05G1358600.v1.1 MD10G1332900.v1.1 MD10G1333000.v1.1
prunus_persica	Prupe.4G009400_v2.0.a1 Prupe.4G009500_v2.0.a1 Prupe.4G009500_v2.0.a1
pyrus_communis	pycom05g32430 pycom10g28220 pycom10g28230 pycom10g28300 pycom10g28330
rosa_chinensis	RchiOBHm_Chr5g0001361 RchiOBHm_Chr5g0001371
rosa_laevigata	RLG00000030922 RLG00000030923
rosa_multiflora	Rmu_co8451069.1_g000001 Rmu_sc0001706.1_g000004 Rmu_sc0003749.1_g000019 Rmu_sc0003900.1_g000003
rosa_roxburghii	Rroxscaffold_1G00074990
rosa_rugosa	Rorug04G0390100.1 Rorug04G0390200
rosa_samantha	Rh5AG010400 Rh5AG010500 Rh5BG013100 Rh5BG013300 Rh5CG011400 Rh5CG011500 Rh5DG010800 Rh5DG010900
rosa_wichuraiana	Rw5G001000 Rw5G001010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1267
Acc36I	ACCTGC	1 cut(s)	1267
AccB7I	CCANNNNNTGG	1 cut(s)	595
AciI	CCGC	7 cut(s)	206, 441, 458, 489, 530, 568, 1468
AclWI	GGATC	2 cut(s)	440, 1179
AcoI	YGGCCR	2 cut(s)	186, 1465
AcuI	CTGAAG	1 cut(s)	730
AdeI	CACNNNGTG	1 cut(s)	520
AfaI	GTAC	2 cut(s)	641, 930
AfiI	CCNNNNNNNGG	5 cut(s)	296, 595, 657, 998, 1553
AflIII	ACRYGT	1 cut(s)	198
AgsI	TTSAA	9 cut(s)	94, 421, 454, 880, 1012, 1346, 1443, 1475, 1510
AjnI	CCWGG	4 cut(s)	429, 752, 843, 1130
AluBI	AGCT	9 cut(s)	78, 172, 366, 717, 734, 889, 1126, 1172, 1559
AluI	AGCT	9 cut(s)	78, 172, 366, 717, 734, 889, 1126, 1172, 1559
Alw21I	GWGCWC	4 cut(s)	149, 668, 891, 1408
Alw26I	GTCTC	1 cut(s)	1274
Alw44I	GTGCAC	1 cut(s)	145
AlwI	GGATC	2 cut(s)	440, 1179
AlwNI	CAGNNNCTG	1 cut(s)	753
AoxI	GGCC	7 cut(s)	178, 186, 243, 414, 841, 1192, 1465
ApaLI	GTGCAC	1 cut(s)	145
ApeKI	GCWGC	4 cut(s)	466, 469, 491, 731
Asp700I	GAANNNNTTC	1 cut(s)	119
AspLEI	GCGC	1 cut(s)	799
AspS9I	GGNCC	5 cut(s)	134, 179, 486, 581, 902
AsuC2I	CCSGG	3 cut(s)	182, 993, 1392
AsuHPI	GGTGA	3 cut(s)	544, 733, 1103
AvaII	GGWCC	4 cut(s)	134, 486, 581, 902
BaeGI	GKGCMC	2 cut(s)	149, 252
BalI	TGGCCA	1 cut(s)	188
BanII	GRGCYC	1 cut(s)	891
BbsI	GAAGAC	1 cut(s)	30
Bbv12I	GWGCWC	4 cut(s)	149, 668, 891, 1408
BbvI	GCAGC	4 cut(s)	453, 456, 503, 743
BccI	CCATC	5 cut(s)	170, 919, 1022, 1096, 1292
BceAI	ACGGC	3 cut(s)	230, 605, 1446
BciT130I	CCWGG	4 cut(s)	431, 754, 845, 1132
BclI	TGATCA	2 cut(s)	1296, 1488
BcnI	CCSGG	3 cut(s)	182, 993, 1392
BcoDI	GTCTC	1 cut(s)	1274
BfaI	CTAG	4 cut(s)	173, 899, 1151, 1220
BfmI	CTRYAG	1 cut(s)	309
BfoI	RGCGCY	1 cut(s)	800
BfuAI	ACCTGC	1 cut(s)	1267
BisI	GCNGC	5 cut(s)	467, 470, 492, 732, 1468
BlsI	GCNGC	5 cut(s)	468, 471, 493, 733, 1469
Bme1390I	CCNGG	7 cut(s)	182, 431, 754, 845, 993, 1132, 1392
Bme18I	GGWCC	4 cut(s)	134, 486, 581, 902
BmgT120I	GGNCC	5 cut(s)	134, 179, 486, 581, 902
BmiI	GGNNCC	3 cut(s)	487, 582, 583
BmrFI	CCNGG	7 cut(s)	182, 431, 754, 845, 993, 1132, 1392
BmrI	ACTGGG	1 cut(s)	1117
BmsI	GCATC	5 cut(s)	501, 531, 936, 1378, 1533
BmuI	ACTGGG	1 cut(s)	1117
BoxI	GACNNNNGTC	1 cut(s)	683
BpiI	GAAGAC	1 cut(s)	30
BpuEI	CTTGAG	2 cut(s)	95, 814
BpuMI	CCSGG	3 cut(s)	182, 993, 1392
BsaBI	GATNNNNATC	1 cut(s)	9
BsaJI	CCNNGG	4 cut(s)	130, 429, 594, 1130
BsaXI	ACNNNNNCTCC	4 cut(s)	1420, 1450, 1548, 1578
Bsc4I	CCNNNNNNNGG	5 cut(s)	296, 595, 657, 998, 1553
Bse118I	RCCGGY	1 cut(s)	554
Bse1I	ACTGG	6 cut(s)	589, 936, 1112, 1188, 1468, 1583
Bse3DI	GCAATG	2 cut(s)	461, 1251
Bse8I	GATNNNNATC	1 cut(s)	9
BseBI	CCWGG	4 cut(s)	431, 754, 845, 1132
BseDI	CCNNGG	4 cut(s)	130, 429, 594, 1130
BseGI	GGATG	9 cut(s)	10, 86, 343, 399, 516, 639, 666, 969, 1369
BseJI	GATNNNNATC	1 cut(s)	9
BseLI	CCNNNNNNNGG	5 cut(s)	296, 595, 657, 998, 1553
BseMI	GCAATG	2 cut(s)	461, 1251
BseMII	CTCAG	2 cut(s)	909, 1281
BseNI	ACTGG	6 cut(s)	589, 936, 1112, 1188, 1468, 1583
BseRI	GAGGAG	1 cut(s)	119
BseSI	GKGCMC	2 cut(s)	149, 252
BseXI	GCAGC	4 cut(s)	453, 456, 503, 743
BseYI	CCCAGC	2 cut(s)	472, 996
Bsh1285I	CGRYCG	1 cut(s)	71
BshFI	GGCC	7 cut(s)	180, 188, 245, 416, 843, 1194, 1467
BsiEI	CGRYCG	1 cut(s)	71
BsiHKAI	GWGCWC	4 cut(s)	149, 668, 891, 1408
BsiSI	CCGG	7 cut(s)	182, 193, 555, 565, 992, 1023, 1391
BslFI	GGGAC	6 cut(s)	256, 499, 567, 691, 1223, 1519
BslI	CCNNNNNNNGG	5 cut(s)	296, 595, 657, 998, 1553
BsmAI	GTCTC	1 cut(s)	1274
BsmFI	GGGAC	6 cut(s)	256, 499, 567, 691, 1223, 1519
BsmI	GAATGC	2 cut(s)	494, 558
BsnI	GGCC	7 cut(s)	180, 188, 245, 416, 843, 1194, 1467
Bsp1286I	GDGCHC	5 cut(s)	149, 252, 668, 891, 1408
Bsp143I	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
BspACI	CCGC	7 cut(s)	206, 441, 458, 489, 530, 568, 1468
BspANI	GGCC	7 cut(s)	180, 188, 245, 416, 843, 1194, 1467
BspCNI	CTCAG	2 cut(s)	910, 1282
BspLI	GGNNCC	3 cut(s)	487, 582, 583
BspMAI	CTGCAG	1 cut(s)	313
BspMI	ACCTGC	1 cut(s)	1267
BspPI	GGATC	2 cut(s)	440, 1179
BspQI	GCTCTTC	1 cut(s)	707
BsrDI	GCAATG	2 cut(s)	461, 1251
BsrFI	RCCGGY	1 cut(s)	554
BsrI	ACTGG	6 cut(s)	589, 936, 1112, 1188, 1468, 1583
BssAI	RCCGGY	1 cut(s)	554
BssECI	CCNNGG	4 cut(s)	130, 429, 594, 1130
BssMI	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
Bst2UI	CCWGG	4 cut(s)	431, 754, 845, 1132
Bst4CI	ACNGT	3 cut(s)	69, 1036, 1450
Bst6I	CTCTTC	5 cut(s)	144, 290, 299, 707, 1013
BstAPI	GCANNNNNTGC	2 cut(s)	612, 1412
BstC8I	GCNNGC	4 cut(s)	190, 604, 1020, 1502
BstDEI	CTNAG	2 cut(s)	918, 1290
BstDSI	CCRYGG	1 cut(s)	594
BstF5I	GGATG	9 cut(s)	10, 86, 343, 399, 516, 639, 666, 969, 1369
BstH2I	RGCGCY	1 cut(s)	800
BstHHI	GCGC	1 cut(s)	799
BstKTI	GATC	6 cut(s)	58, 448, 741, 1187, 1299, 1491
BstMAI	GTCTC	1 cut(s)	1274
BstMBI	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
BstMCI	CGRYCG	1 cut(s)	71
BstMWI	GCNNNNNNNGC	6 cut(s)	466, 612, 803, 895, 1163, 1412
BstNI	CCWGG	4 cut(s)	431, 754, 845, 1132
BstNSI	RCATGY	3 cut(s)	202, 508, 1367
BstPAI	GACNNNNGTC	1 cut(s)	683
BstSCI	CCNGG	7 cut(s)	180, 429, 752, 843, 991, 1130, 1390
BstSFI	CTRYAG	1 cut(s)	309
BstSLI	GKGCMC	2 cut(s)	149, 252
BstV1I	GCAGC	4 cut(s)	453, 456, 503, 743
BstV2I	GAAGAC	1 cut(s)	30
BstX2I	RGATCY	1 cut(s)	445
BstYI	RGATCY	1 cut(s)	445
BsuRI	GGCC	7 cut(s)	180, 188, 245, 416, 843, 1194, 1467
BtgI	CCRYGG	1 cut(s)	594
BtsCI	GGATG	9 cut(s)	10, 86, 343, 399, 516, 639, 666, 969, 1369
BtsIMutI	CAGTG	4 cut(s)	1045, 1195, 1287, 1461
BveI	ACCTGC	1 cut(s)	1267
Cac8I	GCNNGC	4 cut(s)	190, 604, 1020, 1502
CaiI	CAGNNNCTG	1 cut(s)	753
CfoI	GCGC	1 cut(s)	799
Cfr10I	RCCGGY	1 cut(s)	554
Cfr13I	GGNCC	5 cut(s)	134, 179, 486, 581, 902
CsiI	ACCWGGT	1 cut(s)	752
Csp6I	GTAC	2 cut(s)	640, 929
CviAII	CATG	6 cut(s)	199, 253, 268, 334, 505, 1364
CviQI	GTAC	2 cut(s)	640, 929
DdeI	CTNAG	2 cut(s)	918, 1290
DpnI	GATC	6 cut(s)	57, 447, 740, 1186, 1298, 1490
DpnII	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
DraIII	CACNNNGTG	1 cut(s)	520
EaeI	YGGCCR	2 cut(s)	186, 1465
Eam1104I	CTCTTC	5 cut(s)	144, 290, 299, 707, 1013
EarI	CTCTTC	5 cut(s)	144, 290, 299, 707, 1013
EciI	GGCGGA	1 cut(s)	447
Ecl136II	GAGCTC	1 cut(s)	889
Eco24I	GRGCYC	1 cut(s)	891
Eco47I	GGWCC	4 cut(s)	134, 486, 581, 902
Eco53kI	GAGCTC	1 cut(s)	889
Eco57I	CTGAAG	1 cut(s)	730
EcoICRI	GAGCTC	1 cut(s)	889
EcoO109I	RGGNCCY	1 cut(s)	581
EcoRII	CCWGG	4 cut(s)	429, 752, 843, 1130
EcoT22I	ATGCAT	1 cut(s)	1365
EcoT38I	GRGCYC	1 cut(s)	891
FaeI	CATG	6 cut(s)	202, 256, 271, 337, 508, 1367
FalI	AAGNNNNNCTT	4 cut(s)	104, 136, 1484, 1516
FaqI	GGGAC	6 cut(s)	256, 499, 567, 691, 1223, 1519
FatI	CATG	6 cut(s)	198, 252, 267, 333, 504, 1363
FauNDI	CATATG	1 cut(s)	1243
FbaI	TGATCA	2 cut(s)	1296, 1488
Fnu4HI	GCNGC	5 cut(s)	467, 470, 492, 732, 1468
FokI	GGATG	8 cut(s)	73, 350, 386, 523, 646, 673, 976, 1356
FriOI	GRGCYC	1 cut(s)	891
Fsp4HI	GCNGC	5 cut(s)	467, 470, 492, 732, 1468
FspBI	CTAG	4 cut(s)	173, 899, 1151, 1220
GlaI	GCGC	1 cut(s)	798
GluI	GCNGC	5 cut(s)	467, 470, 492, 732, 1468
GsaI	CCCAGC	2 cut(s)	476, 1000
HaeII	RGCGCY	1 cut(s)	800
HaeIII	GGCC	7 cut(s)	180, 188, 245, 416, 843, 1194, 1467
HapII	CCGG	7 cut(s)	182, 193, 555, 565, 992, 1023, 1391
HhaI	GCGC	1 cut(s)	799
Hin1II	CATG	6 cut(s)	202, 256, 271, 337, 508, 1367
Hin6I	GCGC	1 cut(s)	797
HinP1I	GCGC	1 cut(s)	797
HindIII	AAGCTT	1 cut(s)	364
HinfI	GANTC	4 cut(s)	454, 625, 1002, 1387
HpaII	CCGG	7 cut(s)	182, 193, 555, 565, 992, 1023, 1391
HphI	GGTGA	3 cut(s)	544, 733, 1103
Hpy166II	GTNNAC	2 cut(s)	147, 1580
Hpy188I	TCNGA	8 cut(s)	22, 134, 299, 672, 738, 1213, 1383, 1556
Hpy188III	TCNNGA	3 cut(s)	112, 584, 1492
Hpy8I	GTNNAC	2 cut(s)	147, 1580
HpyAV	CCTTC	7 cut(s)	362, 754, 778, 783, 877, 1388, 1449
HpyCH4III	ACNGT	3 cut(s)	69, 1036, 1450
HpyCH4IV	ACGT	1 cut(s)	519
HpyCH4V	TGCA	9 cut(s)	147, 311, 514, 615, 1052, 1119, 1256, 1363, 1369
HpyF10VI	GCNNNNNNNGC	6 cut(s)	466, 612, 803, 895, 1163, 1412
HpyF3I	CTNAG	2 cut(s)	918, 1290
HpySE526I	ACGT	1 cut(s)	519
Hsp92II	CATG	6 cut(s)	202, 256, 271, 337, 508, 1367
HspAI	GCGC	1 cut(s)	797
KflI	GGGWCCC	1 cut(s)	581
Ksp22I	TGATCA	2 cut(s)	1296, 1488
Kzo9I	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
LguI	GCTCTTC	1 cut(s)	707
LmnI	GCTCC	4 cut(s)	739, 886, 1403, 1556
Lsp1109I	GCAGC	4 cut(s)	453, 456, 503, 743
LweI	GCATC	5 cut(s)	501, 531, 936, 1378, 1533
MabI	ACCWGGT	1 cut(s)	752
MaeI	CTAG	4 cut(s)	173, 899, 1151, 1220
MaeII	ACGT	1 cut(s)	519
MaeIII	GTNAC	1 cut(s)	1146
MalI	GATC	6 cut(s)	57, 447, 740, 1186, 1298, 1490
MboI	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
MflI	RGATCY	1 cut(s)	445
MhlI	GDGCHC	5 cut(s)	149, 252, 668, 891, 1408
MlsI	TGGCCA	1 cut(s)	188
MluCI	AATT	7 cut(s)	99, 258, 300, 893, 1341, 1347, 1409
MluNI	TGGCCA	1 cut(s)	188
MlyI	GAGTC	1 cut(s)	1011
MmeI	TCCRAC	3 cut(s)	88, 599, 682
Mox20I	TGGCCA	1 cut(s)	188
Mph1103I	ATGCAT	1 cut(s)	1365
MroXI	GAANNNNTTC	1 cut(s)	119
MscI	TGGCCA	1 cut(s)	188
MseI	TTAA	2 cut(s)	1569, 1588
MslI	CAYNNNNRTG	2 cut(s)	503, 509
Msp20I	TGGCCA	1 cut(s)	188
MspI	CCGG	7 cut(s)	182, 193, 555, 565, 992, 1023, 1391
MspR9I	CCNGG	7 cut(s)	182, 431, 754, 845, 993, 1132, 1392
Mva1269I	GAATGC	2 cut(s)	494, 558
MvaI	CCWGG	4 cut(s)	431, 754, 845, 1132
MwoI	GCNNNNNNNGC	6 cut(s)	466, 612, 803, 895, 1163, 1412
NciI	CCSGG	3 cut(s)	182, 993, 1392
NdeI	CATATG	1 cut(s)	1243
NdeII	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
NlaIII	CATG	6 cut(s)	202, 256, 271, 337, 508, 1367
NlaIV	GGNNCC	3 cut(s)	487, 582, 583
NsiI	ATGCAT	1 cut(s)	1365
NspI	RCATGY	3 cut(s)	202, 508, 1367
PaqCI	CACCTGC	1 cut(s)	1267
PciI	ACATGT	1 cut(s)	198
PciSI	GCTCTTC	1 cut(s)	707
PctI	GAATGC	2 cut(s)	494, 558
PdmI	GAANNNNTTC	1 cut(s)	119
PfeI	GAWTC	3 cut(s)	454, 625, 1387
PflMI	CCANNNNNTGG	1 cut(s)	595
PkrI	GCNGC	5 cut(s)	468, 471, 493, 733, 1469
PleI	GAGTC	1 cut(s)	1010
PpsI	GAGTC	1 cut(s)	1010
PpuMI	RGGWCCY	1 cut(s)	581
PscI	ACATGT	1 cut(s)	198
PshAI	GACNNNNGTC	1 cut(s)	683
Psp124BI	GAGCTC	1 cut(s)	891
Psp5II	RGGWCCY	1 cut(s)	581
Psp6I	CCWGG	4 cut(s)	429, 752, 843, 1130
PspFI	CCCAGC	2 cut(s)	472, 996
PspGI	CCWGG	4 cut(s)	429, 752, 843, 1130
PspN4I	GGNNCC	3 cut(s)	487, 582, 583
PspPI	GGNCC	5 cut(s)	134, 179, 486, 581, 902
PspPPI	RGGWCCY	1 cut(s)	581
PstI	CTGCAG	1 cut(s)	313
PstNI	CAGNNNCTG	1 cut(s)	753
PsuI	RGATCY	1 cut(s)	445
RsaI	GTAC	2 cut(s)	641, 930
RsaNI	GTAC	2 cut(s)	640, 929
RseI	CAYNNNNRTG	2 cut(s)	503, 509
SacI	GAGCTC	1 cut(s)	891
SapI	GCTCTTC	1 cut(s)	707
SaqAI	TTAA	2 cut(s)	1569, 1588
SatI	GCNGC	5 cut(s)	467, 470, 492, 732, 1468
Sau3AI	GATC	6 cut(s)	55, 445, 738, 1184, 1296, 1488
Sau96I	GGNCC	5 cut(s)	134, 179, 486, 581, 902
SchI	GAGTC	1 cut(s)	1011
ScrFI	CCNGG	7 cut(s)	182, 431, 754, 845, 993, 1132, 1392
SduI	GDGCHC	5 cut(s)	149, 252, 668, 891, 1408
SexAI	ACCWGGT	1 cut(s)	752
SfaNI	GCATC	5 cut(s)	501, 531, 936, 1378, 1533
SfcI	CTRYAG	1 cut(s)	309
SinI	GGWCC	4 cut(s)	134, 486, 581, 902
SmiMI	CAYNNNNRTG	2 cut(s)	503, 509
SmlI	CTYRAG	2 cut(s)	110, 829
SmoI	CTYRAG	2 cut(s)	110, 829
Sse9I	AATT	7 cut(s)	99, 258, 300, 893, 1341, 1347, 1409
SsiI	CCGC	7 cut(s)	206, 441, 458, 489, 530, 568, 1468
SspMI	CTAG	4 cut(s)	173, 899, 1151, 1220
SstI	GAGCTC	1 cut(s)	891
StyD4I	CCNGG	7 cut(s)	180, 429, 752, 843, 991, 1130, 1390
TaaI	ACNGT	3 cut(s)	69, 1036, 1450
TaiI	ACGT	1 cut(s)	522
TaqI	TCGA	3 cut(s)	891, 944, 1183
TasI	AATT	7 cut(s)	99, 258, 300, 893, 1341, 1347, 1409
TatI	WGTACW	1 cut(s)	639
TauI	GCSGC	1 cut(s)	1470
TfiI	GAWTC	3 cut(s)	454, 625, 1387
Tru1I	TTAA	2 cut(s)	1569, 1588
Tru9I	TTAA	2 cut(s)	1569, 1588
TscAI	CASTG	4 cut(s)	1045, 1195, 1294, 1468
TseI	GCWGC	4 cut(s)	466, 469, 491, 731
TspDTI	ATGAA	3 cut(s)	75, 487, 1054
TspGWI	ACGGA	4 cut(s)	254, 583, 995, 1191
TspRI	CASTG	4 cut(s)	1045, 1195, 1294, 1468
Van91I	CCANNNNNTGG	1 cut(s)	595
VneI	GTGCAC	1 cut(s)	145
VpaK11BI	GGWCC	4 cut(s)	134, 486, 581, 902
XceI	RCATGY	3 cut(s)	202, 508, 1367
XmnI	GAANNNNTTC	1 cut(s)	119
XspI	CTAG	4 cut(s)	173, 899, 1151, 1220
Zsp2I	ATGCAT	1 cut(s)	1365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.