MD06G1070500.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012611
Physical Location & Seq
Forward (+)
17079281 .. 17080485
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1070500.v1.1.491

Sequence Viewer

Length: 810 bp
ATGCAGGGAAACTCGGTTAACAATTTCGACCTTCATGGTAAGTCGTTTCCCCTAATTTATGAAGGAGATGCTGCAAACTTCTTTGCAGGTGCTAACTCAGCGATATCAAGATATTGTACTAATGGAGTCATGAATTCACATAAACTGAAGGGAAGATTGTTTTCTGCGAAGCCATCGAGAATGGTTTTGGCATCGTGGAATCCAGTTGCAACAATTCTGGATGCTGAGACGGAGGAGGACGTCATGGCACCGTACGTTGTATCGTTTTCTACTAAGGGACCCAACGCCATAACCCTGGATGTGCTAAAGCCTGATCTCACTGCTCCGGGTGTTGATATACTTGCTGCTTGGTCTCCCGTGGCACCGCCTTCCATTGACTTCCACGACACCAAGAGTGTCGACTATAACATAATCTCAGGGACTTCCATGTCCTGTCCTCGTGCTAGTGTTGCTGCTGCGTTTGTCAAGGCTGCCCATCCAGAATGGTTGGCTGCCTCTATCAAATCTGCCGTATTGACTACAGCTCGTGTCCTGGACCCAAACAAGCACAATGAACTTGAATTTGCTTACGGGTTTGGTCACATCAACTCTCTGAAAGCAGTCAAACCCGGGCTGGTCTTTGATCTATACGAAGCAGACTACGTGCATTTCCTCTGCAAACAGGGTTACAACAGCACAACATTAAAACTTATCACTGGTGACAACGGCAGCGGCTGTGGCAGAACAAAGCCAGGAAGAGCTTGGGATCTGAATAACCCATCCTTCTCCCTTGCAGTTGAAGATGGCCAGCCAGTGACAACATCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

270

Amino Acids

28.82

Weight (kDa)

6.15

Isoelectric Point (pI)

26.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 82 - 193 7e-17 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
AasI GACNNNNNNGTC 1 cut(s) 427
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 77
AccB1I GGYRCC 2 cut(s) 247, 361
AccI GTMKAC 1 cut(s) 399
AciI CCGC 2 cut(s) 365, 711
AclWI GGATC 1 cut(s) 753
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 2 cut(s) 133, 560
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 118, 254
AgsI TTSAA 2 cut(s) 560, 779
AjnI CCWGG 3 cut(s) 294, 531, 730
AluBI AGCT 2 cut(s) 524, 740
AluI AGCT 2 cut(s) 524, 740
Alw26I GTCTC 2 cut(s) 221, 357
AlwI GGATC 1 cut(s) 753
AlwNI CAGNNNCTG 1 cut(s) 714
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 1 cut(s) 784
ApeKI GCWGC 7 cut(s) 71, 344, 452, 455, 470, 491, 708
ApoI RAATTY 2 cut(s) 133, 560
AspS9I GGNCC 2 cut(s) 278, 535
AsuC2I CCSGG 3 cut(s) 327, 609, 610
AsuHPI GGTGA 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 2 cut(s) 278, 535
BalI TGGCCA 1 cut(s) 786
BanI GGYRCC 2 cut(s) 247, 361
BauI CACGAG 2 cut(s) 438, 525
BbvI GCAGC 7 cut(s) 58, 331, 439, 442, 457, 478, 720
BccI CCATC 4 cut(s) 181, 483, 766, 776
BceAI ACGGC 2 cut(s) 494, 721
BciT130I CCWGG 3 cut(s) 296, 533, 732
BcnI CCSGG 3 cut(s) 327, 609, 610
BcoDI GTCTC 2 cut(s) 221, 357
BfaI CTAG 2 cut(s) 444, 804
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 8 cut(s) 72, 345, 453, 456, 471, 492, 709, 712
BlsI GCNGC 8 cut(s) 73, 346, 454, 457, 472, 493, 710, 713
Bme1390I CCNGG 6 cut(s) 296, 327, 533, 609, 610, 732
Bme18I GGWCC 2 cut(s) 278, 535
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 2 cut(s) 278, 535
BmiI GGNNCC 5 cut(s) 249, 279, 280, 363, 537
BmrFI CCNGG 6 cut(s) 296, 327, 533, 609, 610, 732
BmsI GCATC 3 cut(s) 58, 200, 211
BpuMI CCSGG 3 cut(s) 327, 609, 610
BsaAI YACGTR 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 294, 357, 608
Bse1I ACTGG 3 cut(s) 203, 700, 791
BseBI CCWGG 3 cut(s) 296, 533, 732
BseDI CCNNGG 3 cut(s) 294, 357, 608
BseGI GGATG 4 cut(s) 226, 304, 475, 758
BseMII CTCAG 3 cut(s) 111, 216, 429
BseNI ACTGG 3 cut(s) 203, 700, 791
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 7 cut(s) 58, 331, 439, 442, 457, 478, 720
BshFI GGCC 1 cut(s) 786
BshNI GGYRCC 2 cut(s) 247, 361
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 2 cut(s) 326, 609
BsiWI CGTACG 1 cut(s) 252
BslFI GGGAC 2 cut(s) 291, 433
BsmAI GTCTC 2 cut(s) 221, 357
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 291, 433
BsnI GGCC 1 cut(s) 786
Bso31I GGTCTC 1 cut(s) 357
BsoBI CYCGRG 1 cut(s) 608
Bsp143I GATC 3 cut(s) 313, 622, 745
BspACI CCGC 2 cut(s) 365, 711
BspANI GGCC 1 cut(s) 786
BspCNI CTCAG 3 cut(s) 110, 217, 428
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 5 cut(s) 249, 279, 280, 363, 537
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 1 cut(s) 753
BspQI GCTCTTC 1 cut(s) 730
BspT107I GGYRCC 2 cut(s) 247, 361
BspTNI GGTCTC 1 cut(s) 357
BsrI ACTGG 3 cut(s) 203, 700, 791
BssECI CCNNGG 3 cut(s) 294, 357, 608
BssMI GATC 3 cut(s) 313, 622, 745
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 2 cut(s) 438, 525
Bst2BI CACGAG 2 cut(s) 438, 525
Bst2UI CCWGG 3 cut(s) 296, 533, 732
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 643
BstC8I GCNNGC 1 cut(s) 788
BstDEI CTNAG 4 cut(s) 97, 225, 273, 415
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 4 cut(s) 226, 304, 475, 758
BstKTI GATC 3 cut(s) 316, 625, 748
BstMAI GTCTC 2 cut(s) 221, 357
BstMBI GATC 3 cut(s) 313, 622, 745
BstMWI GCNNNNNNNGC 3 cut(s) 98, 449, 717
BstNI CCWGG 3 cut(s) 296, 533, 732
BstSCI CCNGG 6 cut(s) 294, 325, 531, 607, 608, 730
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 7 cut(s) 58, 331, 439, 442, 457, 478, 720
BstX2I RGATCY 1 cut(s) 745
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 745
BsuRI GGCC 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 4 cut(s) 226, 304, 475, 758
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 318, 693, 798
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 788
CaiI CAGNNNCTG 1 cut(s) 714
CciI TCATGA 1 cut(s) 129
Cfr13I GGNCC 2 cut(s) 278, 535
Cfr9I CCCGGG 1 cut(s) 608
Csp6I GTAC 2 cut(s) 117, 253
CviAII CATG 4 cut(s) 35, 130, 244, 427
CviQI GTAC 2 cut(s) 117, 253
DdeI CTNAG 4 cut(s) 97, 225, 273, 415
DpnI GATC 3 cut(s) 315, 624, 747
DpnII GATC 3 cut(s) 313, 622, 745
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
Eco31I GGTCTC 1 cut(s) 357
Eco32I GATATC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 278, 535
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 3 cut(s) 294, 531, 730
EcoRV GATATC 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 4 cut(s) 38, 133, 247, 430
FaqI GGGAC 2 cut(s) 291, 433
FatI CATG 4 cut(s) 34, 129, 243, 426
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 8 cut(s) 72, 345, 453, 456, 471, 492, 709, 712
FokI GGATG 4 cut(s) 233, 311, 462, 745
Fsp4HI GCNGC 8 cut(s) 72, 345, 453, 456, 471, 492, 709, 712
FspBI CTAG 2 cut(s) 444, 804
GluI GCNGC 8 cut(s) 72, 345, 453, 456, 471, 492, 709, 712
HaeIII GGCC 1 cut(s) 786
HapII CCGG 2 cut(s) 326, 609
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 4 cut(s) 38, 133, 247, 430
HincII GTYRAC 2 cut(s) 19, 400
HindII GTYRAC 2 cut(s) 19, 400
HinfI GANTC 2 cut(s) 126, 199
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 2 cut(s) 326, 609
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 2 cut(s) 19, 400
Hpy188I TCNGA 2 cut(s) 594, 750
Hpy188III TCNNGA 5 cut(s) 108, 130, 177, 218, 479
Hpy8I GTNNAC 2 cut(s) 19, 400
HpyAV CCTTC 5 cut(s) 41, 56, 142, 378, 772
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4IV ACGT 3 cut(s) 240, 255, 642
HpyCH4V TGCA 7 cut(s) 4, 74, 86, 209, 646, 657, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 449, 717
HpyF3I CTNAG 4 cut(s) 97, 225, 273, 415
HpySE526I ACGT 3 cut(s) 240, 255, 642
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 4 cut(s) 38, 133, 247, 430
KflI GGGWCCC 1 cut(s) 278
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 3 cut(s) 313, 622, 745
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 7 cut(s) 58, 331, 439, 442, 457, 478, 720
LweI GCATC 3 cut(s) 58, 200, 211
MaeI CTAG 2 cut(s) 444, 804
MaeII ACGT 3 cut(s) 240, 255, 642
MaeIII GTNAC 4 cut(s) 578, 665, 698, 793
MalI GATC 3 cut(s) 315, 624, 747
MboI GATC 3 cut(s) 313, 622, 745
MboII GAAGA 3 cut(s) 165, 747, 791
MflI RGATCY 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 5 cut(s) 22, 54, 133, 213, 560
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 1 cut(s) 135
MnlI CCTC 5 cut(s) 226, 229, 447, 505, 662
Mox20I TGGCCA 1 cut(s) 786
MscI TGGCCA 1 cut(s) 786
MseI TTAA 3 cut(s) 18, 683, 808
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 711
MspI CCGG 2 cut(s) 326, 609
MspR9I CCNGG 6 cut(s) 296, 327, 533, 609, 610, 732
MvaI CCWGG 3 cut(s) 296, 533, 732
MwoI GCNNNNNNNGC 3 cut(s) 98, 449, 717
NciI CCSGG 3 cut(s) 327, 609, 610
NdeII GATC 3 cut(s) 313, 622, 745
NlaIII CATG 4 cut(s) 38, 133, 247, 430
NlaIV GGNNCC 5 cut(s) 249, 279, 280, 363, 537
NmuCI GTSAC 3 cut(s) 578, 698, 793
PagI TCATGA 1 cut(s) 129
PaqCI CACCTGC 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 730
PfeI GAWTC 1 cut(s) 199
Pfl23II CGTACG 1 cut(s) 252
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 8 cut(s) 73, 346, 454, 457, 472, 493, 710, 713
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Ppu21I YACGTR 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 3 cut(s) 294, 531, 730
PspGI CCWGG 3 cut(s) 294, 531, 730
PspLI CGTACG 1 cut(s) 252
PspN4I GGNNCC 5 cut(s) 249, 279, 280, 363, 537
PspPI GGNCC 2 cut(s) 278, 535
PspPPI RGGWCCY 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 714
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 2 cut(s) 118, 254
RsaNI GTAC 2 cut(s) 117, 253
SalI GTCGAC 1 cut(s) 398
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 3 cut(s) 18, 683, 808
SatI GCNGC 8 cut(s) 72, 345, 453, 456, 471, 492, 709, 712
Sau3AI GATC 3 cut(s) 313, 622, 745
Sau96I GGNCC 2 cut(s) 278, 535
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 6 cut(s) 296, 327, 533, 609, 610, 732
SetI ASST 7 cut(s) 33, 91, 243, 258, 526, 645, 742
SfaNI GCATC 3 cut(s) 58, 200, 211
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 2 cut(s) 278, 535
SmaI CCCGGG 1 cut(s) 610
Sse9I AATT 5 cut(s) 22, 54, 133, 213, 560
SsiI CCGC 2 cut(s) 365, 711
SspMI CTAG 2 cut(s) 444, 804
StyD4I CCNGG 6 cut(s) 294, 325, 531, 607, 608, 730
TaaI ACNGT 1 cut(s) 252
TaiI ACGT 3 cut(s) 243, 258, 645
TaqI TCGA 3 cut(s) 27, 176, 399
TasI AATT 5 cut(s) 22, 54, 133, 213, 560
TatI WGTACW 1 cut(s) 116
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 3 cut(s) 18, 683, 808
Tru9I TTAA 3 cut(s) 18, 683, 808
TscAI CASTG 3 cut(s) 325, 700, 798
TseFI GTSAC 3 cut(s) 578, 698, 793
TseI GCWGC 7 cut(s) 71, 344, 452, 455, 470, 491, 708
Tsp45I GTSAC 3 cut(s) 578, 698, 793
TspDTI ATGAA 4 cut(s) 23, 75, 146, 567
TspGWI ACGGA 1 cut(s) 245
TspMI CCCGGG 1 cut(s) 608
TspRI CASTG 3 cut(s) 325, 700, 798
VpaK11BI GGWCC 2 cut(s) 278, 535
XapI RAATTY 2 cut(s) 133, 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 738
XmaI CCCGGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 444, 804
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.