Rh7BG183400

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012611
Physical Location & Seq
Forward (+)
14709356 .. 14712523
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG183400.1

Sequence Viewer

Length: 2088 bp
ATGGCCAAGAGAGGAAGGTTCACATTGTATACATGGGAGAGCGGCACCATGGAGATTATTCTGCTCAATCCACACACCATTCAGTGCTACAAAGCCTTCAAGTCGGCCAAAGAGTCGCTGATCTATAGTTATGGGAAGAGCTTTAATGGGTTTGCCGCAAAGTTGTCGGATGAAGAAGTTGCAGAGTTGGCAGAAATGGAAGGTATAGTTTCAGTGATTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACTTGGAGCATCTGTAGGAGGAGAGGTTGTGATAGGGCTTCTAGACTCAGGAATCTGGCCGGAATCTGATAGCTTCAGTGATGATGGCTTTGGCCCTCCACCTAAGAAATGGAAAGGCAAATGCAAAGGTGCCAACTTCACCTGCAACAACAAAGTTATTGGAGCCCGCTACTACAACAGTGATGGCTCCTATGACCCCACTGACATCCAGTCCCCAAGAGATTCCAACGGACATGGAAGCCACACTGCTTCAACTGCAGCAGGCCGCGAGGTGCCAGCCAGTTACTTGGGGTTAGCTTCTGGAACTGTAAGAGGTGGAGTCCCGAATGCTAGGATTGCTGTCTACAAAGTTTGCTGGGCATCTGGATGTTATTCTTCAGATGTCCTTGCAGCATTCGATGATGCAATAGCAGATGGAGTTGATATTATATCGGTCTCACTTGGCAGTGACTTCCCACTCCAATACTTAGAAGACCCCATTGCTATTGGGTCTTTCCATGCCATGAAATATGGTATAGTGACCTCAACTTCTGCTGGAAACTCTGGCCCTGCTCCATTATCAGTTTCTAATTATGCACCATGGATAATGACCGTTGCTGCCAGCACCATTGACAGAAAATTTGTGGCAAAAGCAGTTCTTGGCAATGGAGAAGTCTATAGTGGACTTTCAATTAATAATTTCGACCTGAATGGAAAGTCATATCCCCTAATATGGGGAGGAGATGCTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGCAAGCTTTTGTCCAAATGGAGCCCTGAATTCACACAAAGTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGACGATGGTTCGGGTGTTCTACTAGCCAATGGTGTTGGTACAATTATGGCTGATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTACAATAGGAGATGGACAGAGAATTATGAACTATATCAGATCAACAGCGAATCCAAATGCAACAATTCTTGTTGGTGAGACGGACATGGATATCATGGCACCTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCTATAACCCCAGATATCCTCAAGCCTGATCTGACCGCCCCTGGTGTTGACATACTTGCTGCTTGGTCTCCAGTGGCACCGCCTTCCATTTACGAAGATGACACCAGGACTCATGTCCTGGACCCAAAGAAGCATGAAGACCTCGAATTCGCTTATGGGTCTGGTCACATCAACCCTCTGAAAGCAGTAGACCCCGGGCTTGTTTTTGATGCACATGTAGCAGACTATGTACATTTCCTCTGCAAGCAGGGTTACAATACCACCACTTTGAAGCTCATCACTGGTGACAACAGCAGTACTTGTGGCAGGACAAAGCCAGGAAGAGCTTGGGATCTTAACTATCCATCGTTCTCTCTAGCAGTTGAAGATGGCCAGCATATCAATGCTGTCTTTACTAGAACAGTCACAAATGTGGGATCACCAAACTCAACCTATACTATTAAGGCGCACACACCCTCCTCAAGTGTTTCGGTAAAACCATCAACCATTTCTTTCTCTTCTGTCGGTGAGAAGAAATCCTTCACAGTCAAGGTAATTGGTCCGCCTATTGCACAGCAGCCGATCATGTCTGGTGCAATCGTGTTGACTGATGGGATCCATCTGGTGAGAAGCCCTCTCGTTATCTACACAATCCTCCCTGGTGCTGCACATTCGTACTCCATACCTGAGAAAAAACCAAGTTTTAAAGGTTCTTCCTTGTACCACAGAAATGGGATCTTCAGACACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

695

Amino Acids

73.94

Weight (kDa)

6.32

Isoelectric Point (pI)

23.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 81 4.2e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 102 - 469 3.1e-26 Subtilase family
fn3_6 PF17766 563 - 660 1.6e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 5 cut(s) 44, 413, 556, 1318, 1432
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 3 cut(s) 29, 627, 1545
AccII CGCG 1 cut(s) 552
AciI CCGC 7 cut(s) 42, 156, 451, 550, 1392, 1436, 1898
AclWI GGATC 5 cut(s) 1695, 1780, 1945, 1958, 2078
AcoI YGGCCR 4 cut(s) 3, 105, 341, 1726
AcsI RAATTY 3 cut(s) 902, 1072, 1502
AcuI CTGAAG 3 cut(s) 343, 645, 2059
AfaI GTAC 5 cut(s) 1156, 1587, 1654, 2012, 2057
AfiI CCNNNNNNNGG 2 cut(s) 996, 997
AflIII ACRYGT 1 cut(s) 1570
AgsI TTSAA 5 cut(s) 100, 537, 954, 1627, 1721
AhdI GACNNNNNGTC 1 cut(s) 493
AjnI CCWGG 5 cut(s) 1396, 1460, 1473, 1672, 1993
AleI CACNNNNGTG 1 cut(s) 1766
AluBI AGCT 8 cut(s) 141, 238, 357, 581, 1031, 1050, 1630, 1682
AluI AGCT 8 cut(s) 141, 238, 357, 581, 1031, 1050, 1630, 1682
Alw26I GTCTC 3 cut(s) 724, 1292, 1428
AlwI GGATC 5 cut(s) 1695, 1780, 1945, 1958, 2078
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 1 cut(s) 1550
AoxI GGCC 8 cut(s) 3, 105, 280, 341, 376, 547, 829, 1726
ApeKI GCWGC 8 cut(s) 542, 674, 881, 1010, 1208, 1415, 1912, 2000
ApoI RAATTY 3 cut(s) 902, 1072, 1502
ArsI GACNNNNNNTTYG 4 cut(s) 887, 919, 1487, 1519
AseI ATTAAT 1 cut(s) 957
Asp700I GAANNNNTTC 1 cut(s) 1874
AspLEI GCGC 1 cut(s) 1804
AspS9I GGNCC 5 cut(s) 377, 830, 1349, 1477, 1895
AsuC2I CCSGG 2 cut(s) 1551, 1552
AsuHPI GGTGA 6 cut(s) 415, 1307, 1652, 1767, 1874, 1972
AvaI CYCGRG 1 cut(s) 1550
AvaII GGWCC 3 cut(s) 1349, 1477, 1895
BalI TGGCCA 2 cut(s) 5, 1728
BamHI GGATCC 1 cut(s) 1950
BanI GGYRCC 5 cut(s) 44, 413, 556, 1318, 1432
BanII GRGCYC 2 cut(s) 451, 1069
BbsI GAAGAC 2 cut(s) 762, 1500
BbvI GCAGC 8 cut(s) 554, 686, 868, 997, 1220, 1402, 1924, 1987
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 5 cut(s) 1398, 1462, 1475, 1674, 1995
BcnI CCSGG 2 cut(s) 1551, 1552
BcoDI GTCTC 3 cut(s) 724, 1292, 1428
BfaI CTAG 6 cut(s) 326, 615, 1139, 1344, 1712, 1752
BfmI CTRYAG 4 cut(s) 124, 297, 540, 940
BfuAI ACCTGC 1 cut(s) 434
BmcAI AGTACT 1 cut(s) 1654
Bme1390I CCNGG 7 cut(s) 1398, 1462, 1475, 1551, 1552, 1674, 1995
Bme18I GGWCC 3 cut(s) 1349, 1477, 1895
BmeRI GACNNNNNGTC 1 cut(s) 493
BmeT110I CYCGRG 1 cut(s) 1550
BmgT120I GGNCC 5 cut(s) 377, 830, 1349, 1477, 1895
BmrFI CCNGG 7 cut(s) 1398, 1462, 1475, 1551, 1552, 1674, 1995
BmsI GCATC 5 cut(s) 302, 653, 676, 997, 1555
BoxI GACNNNNGTC 1 cut(s) 1469
BpiI GAAGAC 2 cut(s) 762, 1500
BplI GAGNNNNNCTC 2 cut(s) 1954, 1986
BpmI CTGGAG 2 cut(s) 1047, 1410
BpuEI CTTGAG 2 cut(s) 1361, 1801
BpuMI CCSGG 2 cut(s) 1551, 1552
BsaI GGTCTC 2 cut(s) 724, 1428
BsaJI CCNNGG 6 cut(s) 48, 863, 1396, 1549, 1550, 1993
BsaXI ACNNNNNCTCC 8 cut(s) 282, 294, 297, 312, 324, 327, 1796, 1826
Bsc4I CCNNNNNNNGG 2 cut(s) 996, 997
Bse1I ACTGG 4 cut(s) 493, 564, 1427, 1642
Bse3DI GCAATG 2 cut(s) 762, 934
BseBI CCWGG 5 cut(s) 1398, 1462, 1475, 1674, 1995
BseDI CCNNGG 6 cut(s) 48, 863, 1396, 1549, 1550, 1993
BseGI GGATG 3 cut(s) 175, 489, 656
BseLI CCNNNNNNNGG 2 cut(s) 996, 997
BseMI GCAATG 2 cut(s) 762, 934
BseMII CTCAG 2 cut(s) 345, 2013
BseNI ACTGG 4 cut(s) 493, 564, 1427, 1642
BseRI GAGGAG 3 cut(s) 318, 1017, 1804
BseXI GCAGC 8 cut(s) 554, 686, 868, 997, 1220, 1402, 1924, 1987
BseYI CCCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 1986
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 8 cut(s) 5, 107, 282, 343, 378, 549, 831, 1728
BshNI GGYRCC 5 cut(s) 44, 413, 556, 1318, 1432
BsiHKCI CYCGRG 1 cut(s) 1550
BsiSI CCGG 2 cut(s) 344, 1551
BslFI GGGAC 3 cut(s) 272, 481, 590
BslI CCNNNNNNNGG 2 cut(s) 996, 997
BsmAI GTCTC 3 cut(s) 724, 1292, 1428
BsmBI CGTCTC 1 cut(s) 1292
BsmFI GGGAC 3 cut(s) 272, 481, 590
BsmI GAATGC 2 cut(s) 616, 677
BsnI GGCC 8 cut(s) 5, 107, 282, 343, 378, 549, 831, 1728
Bso31I GGTCTC 2 cut(s) 724, 1428
BsoBI CYCGRG 1 cut(s) 1550
Bsp1286I GDGCHC 2 cut(s) 451, 1069
Bsp1407I TGTACA 1 cut(s) 1585
Bsp143I GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
Bsp19I CCATGG 2 cut(s) 48, 863
BspACI CCGC 7 cut(s) 42, 156, 451, 550, 1392, 1436, 1898
BspANI GGCC 8 cut(s) 5, 107, 282, 343, 378, 549, 831, 1728
BspCNI CTCAG 2 cut(s) 344, 2014
BspFNI CGCG 1 cut(s) 552
BspMAI CTGCAG 1 cut(s) 544
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 5 cut(s) 1695, 1780, 1945, 1958, 2078
BspQI GCTCTTC 2 cut(s) 131, 1672
BspT107I GGYRCC 5 cut(s) 44, 413, 556, 1318, 1432
BspTNI GGTCTC 2 cut(s) 724, 1428
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 762, 934
BsrGI TGTACA 1 cut(s) 1585
BsrI ACTGG 4 cut(s) 493, 564, 1427, 1642
BssECI CCNNGG 6 cut(s) 48, 863, 1396, 1549, 1550, 1993
BssMI GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
BssNAI GTATAC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 48, 863
Bst1107I GTATAC 1 cut(s) 30
Bst2UI CCWGG 5 cut(s) 1398, 1462, 1475, 1674, 1995
Bst4CI ACNGT 6 cut(s) 464, 592, 877, 1216, 1759, 1882
Bst6I CTCTTC 3 cut(s) 131, 1672, 1858
BstAUI TGTACA 1 cut(s) 1585
BstC8I GCNNGC 7 cut(s) 451, 547, 561, 886, 1048, 1601, 1730
BstDEI CTNAG 4 cut(s) 331, 387, 751, 2022
BstDSI CCRYGG 2 cut(s) 48, 863
BstF5I GGATG 3 cut(s) 175, 489, 656
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 1804
BstKTI GATC 8 cut(s) 123, 1262, 1387, 1690, 1775, 1920, 1953, 2073
BstMAI GTCTC 3 cut(s) 724, 1292, 1428
BstMBI GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
BstMWI GCNNNNNNNGC 4 cut(s) 188, 539, 620, 1574
BstNI CCWGG 5 cut(s) 1398, 1462, 1475, 1674, 1995
BstNSI RCATGY 1 cut(s) 1574
BstPAI GACNNNNGTC 1 cut(s) 1469
BstSCI CCNGG 7 cut(s) 1396, 1460, 1473, 1549, 1550, 1672, 1993
BstSFI CTRYAG 4 cut(s) 124, 297, 540, 940
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 8 cut(s) 554, 686, 868, 997, 1220, 1402, 1924, 1987
BstV2I GAAGAC 2 cut(s) 762, 1500
BstX2I RGATCY 3 cut(s) 1687, 1950, 2070
BstXI CCANNNNNNTGG 2 cut(s) 571, 2066
BstYI RGATCY 3 cut(s) 1687, 1950, 2070
BstZ17I GTATAC 1 cut(s) 30
BsuRI GGCC 8 cut(s) 5, 107, 282, 343, 378, 549, 831, 1728
BtgI CCRYGG 2 cut(s) 48, 863
BtsCI GGATG 3 cut(s) 175, 489, 656
BtsI GCAGTG 2 cut(s) 528, 736
BtsIMutI CAGTG 9 cut(s) 89, 219, 367, 469, 483, 528, 736, 1434, 1635
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 7 cut(s) 451, 547, 561, 886, 1048, 1601, 1730
CaiI CAGNNNCTG 1 cut(s) 1214
CfoI GCGC 1 cut(s) 1804
Cfr13I GGNCC 5 cut(s) 377, 830, 1349, 1477, 1895
Cfr9I CCCGGG 1 cut(s) 1550
Csp6I GTAC 5 cut(s) 1155, 1586, 1653, 2011, 2056
CviQI GTAC 5 cut(s) 1155, 1586, 1653, 2011, 2056
DdeI CTNAG 4 cut(s) 331, 387, 751, 2022
DpnI GATC 8 cut(s) 122, 1261, 1386, 1689, 1774, 1919, 1952, 2072
DpnII GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
DraI TTTAAA 1 cut(s) 2041
DriI GACNNNNNGTC 1 cut(s) 493
EaeI YGGCCR 4 cut(s) 3, 105, 341, 1726
Eam1104I CTCTTC 3 cut(s) 131, 1672, 1858
Eam1105I GACNNNNNGTC 1 cut(s) 493
EarI CTCTTC 3 cut(s) 131, 1672, 1858
EciI GGCGGA 1 cut(s) 1887
Eco130I CCWWGG 2 cut(s) 48, 863
Eco24I GRGCYC 2 cut(s) 451, 1069
Eco31I GGTCTC 2 cut(s) 724, 1428
Eco32I GATATC 2 cut(s) 1312, 1372
Eco47I GGWCC 3 cut(s) 1349, 1477, 1895
Eco57I CTGAAG 3 cut(s) 343, 645, 2059
Eco88I CYCGRG 1 cut(s) 1550
EcoO109I RGGNCCY 1 cut(s) 1349
EcoRI GAATTC 2 cut(s) 1072, 1502
EcoRII CCWGG 5 cut(s) 1396, 1460, 1473, 1672, 1993
EcoRV GATATC 2 cut(s) 1312, 1372
EcoT14I CCWWGG 2 cut(s) 48, 863
EcoT38I GRGCYC 2 cut(s) 451, 1069
ErhI CCWWGG 2 cut(s) 48, 863
Esp3I CGTCTC 1 cut(s) 1292
FalI AAGNNNNNCTT 6 cut(s) 270, 302, 906, 938, 1859, 1891
FaqI GGGAC 3 cut(s) 272, 481, 590
FauI CCCGC 1 cut(s) 458
FblI GTMKAC 3 cut(s) 29, 627, 1545
FokI GGATG 3 cut(s) 182, 476, 663
FriOI GRGCYC 2 cut(s) 451, 1069
FspBI CTAG 6 cut(s) 326, 615, 1139, 1344, 1712, 1752
GlaI GCGC 1 cut(s) 1803
GsaI CCCAGC 1 cut(s) 643
GsuI CTGGAG 2 cut(s) 1047, 1410
HaeIII GGCC 8 cut(s) 5, 107, 282, 343, 378, 549, 831, 1728
HapII CCGG 2 cut(s) 344, 1551
HhaI GCGC 1 cut(s) 1804
Hin6I GCGC 1 cut(s) 1802
HinP1I GCGC 1 cut(s) 1802
HincII GTYRAC 2 cut(s) 1405, 1941
HindII GTYRAC 2 cut(s) 1405, 1941
HindIII AAGCTT 2 cut(s) 236, 1048
HpaII CCGG 2 cut(s) 344, 1551
HphI GGTGA 6 cut(s) 415, 1307, 1652, 1767, 1874, 1972
Hpy166II GTNNAC 7 cut(s) 21, 30, 628, 947, 1405, 1546, 1941
Hpy188I TCNGA 9 cut(s) 169, 352, 664, 1039, 1183, 1259, 1389, 1536, 2078
Hpy188III TCNNGA 6 cut(s) 326, 333, 585, 607, 648, 1344
Hpy8I GTNNAC 7 cut(s) 21, 30, 628, 947, 1405, 1546, 1941
HpyAV CCTTC 5 cut(s) 9, 106, 194, 1449, 1885
HpyCH4III ACNGT 6 cut(s) 464, 592, 877, 1216, 1759, 1882
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 539, 620, 1574
HpyF3I CTNAG 4 cut(s) 331, 387, 751, 2022
HspAI GCGC 1 cut(s) 1802
Kzo9I GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
LguI GCTCTTC 2 cut(s) 131, 1672
LmnI GCTCC 6 cut(s) 290, 446, 476, 841, 1028, 1064
Lsp1109I GCAGC 8 cut(s) 554, 686, 868, 997, 1220, 1402, 1924, 1987
LweI GCATC 5 cut(s) 302, 653, 676, 997, 1555
MaeI CTAG 6 cut(s) 326, 615, 1139, 1344, 1712, 1752
MaeIII GTNAC 8 cut(s) 566, 731, 802, 1219, 1520, 1607, 1640, 1759
MalI GATC 8 cut(s) 122, 1261, 1386, 1689, 1774, 1919, 1952, 2072
MbiI CCGCTC 1 cut(s) 42
MboI GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
MflI RGATCY 3 cut(s) 1687, 1950, 2070
MhlI GDGCHC 2 cut(s) 451, 1069
MlsI TGGCCA 2 cut(s) 5, 1728
MluNI TGGCCA 2 cut(s) 5, 1728
MlyI GAGTC 4 cut(s) 122, 323, 612, 1459
MmeI TCCRAC 2 cut(s) 147, 534
Mox20I TGGCCA 2 cut(s) 5, 1728
MroXI GAANNNNTTC 1 cut(s) 1874
MscI TGGCCA 2 cut(s) 5, 1728
MseI TTAA 6 cut(s) 144, 957, 1194, 1692, 1797, 2040
MslI CAYNNNNRTG 4 cut(s) 649, 1737, 1766, 2064
Msp20I TGGCCA 2 cut(s) 5, 1728
MspI CCGG 2 cut(s) 344, 1551
MspR9I CCNGG 7 cut(s) 1398, 1462, 1475, 1551, 1552, 1674, 1995
Mva1269I GAATGC 2 cut(s) 616, 677
MvaI CCWGG 5 cut(s) 1398, 1462, 1475, 1674, 1995
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 4 cut(s) 188, 539, 620, 1574
NciI CCSGG 2 cut(s) 1551, 1552
NcoI CCATGG 2 cut(s) 48, 863
NdeII GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
NmuCI GTSAC 5 cut(s) 731, 802, 1520, 1640, 1759
NspI RCATGY 1 cut(s) 1574
OliI CACNNNNGTG 1 cut(s) 1766
PaqCI CACCTGC 1 cut(s) 434
PciI ACATGT 1 cut(s) 1570
PciSI GCTCTTC 2 cut(s) 131, 1672
PctI GAATGC 2 cut(s) 616, 677
PdmI GAANNNNTTC 1 cut(s) 1874
PfeI GAWTC 6 cut(s) 217, 336, 347, 506, 1169, 1270
PfoI TCCNGGA 1 cut(s) 1473
PleI GAGTC 4 cut(s) 121, 323, 611, 1459
PpsI GAGTC 4 cut(s) 121, 323, 611, 1459
PpuMI RGGWCCY 1 cut(s) 1349
PscI ACATGT 1 cut(s) 1570
PshAI GACNNNNGTC 1 cut(s) 1469
PshBI ATTAAT 1 cut(s) 957
Psp5II RGGWCCY 1 cut(s) 1349
Psp6I CCWGG 5 cut(s) 1396, 1460, 1473, 1672, 1993
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 5 cut(s) 1396, 1460, 1473, 1672, 1993
PspPI GGNCC 5 cut(s) 377, 830, 1349, 1477, 1895
PspPPI RGGWCCY 1 cut(s) 1349
PstI CTGCAG 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 3 cut(s) 1687, 1950, 2070
RsaI GTAC 5 cut(s) 1156, 1587, 1654, 2012, 2057
RsaNI GTAC 5 cut(s) 1155, 1586, 1653, 2011, 2056
RseI CAYNNNNRTG 4 cut(s) 649, 1737, 1766, 2064
SapI GCTCTTC 2 cut(s) 131, 1672
SaqAI TTAA 6 cut(s) 144, 957, 1194, 1692, 1797, 2040
Sau3AI GATC 8 cut(s) 120, 1259, 1384, 1687, 1772, 1917, 1950, 2070
Sau96I GGNCC 5 cut(s) 377, 830, 1349, 1477, 1895
ScaI AGTACT 1 cut(s) 1654
SchI GAGTC 4 cut(s) 122, 323, 612, 1459
ScrFI CCNGG 7 cut(s) 1398, 1462, 1475, 1551, 1552, 1674, 1995
SduI GDGCHC 2 cut(s) 451, 1069
SfaNI GCATC 5 cut(s) 302, 653, 676, 997, 1555
SfcI CTRYAG 4 cut(s) 124, 297, 540, 940
SinI GGWCC 3 cut(s) 1349, 1477, 1895
SmaI CCCGGG 1 cut(s) 1552
SmiMI CAYNNNNRTG 4 cut(s) 649, 1737, 1766, 2064
SmlI CTYRAG 2 cut(s) 1376, 1816
SmoI CTYRAG 2 cut(s) 1376, 1816
SsiI CCGC 7 cut(s) 42, 156, 451, 550, 1392, 1436, 1898
SspMI CTAG 6 cut(s) 326, 615, 1139, 1344, 1712, 1752
StyD4I CCNGG 7 cut(s) 1396, 1460, 1473, 1549, 1550, 1672, 1993
StyI CCWWGG 2 cut(s) 48, 863
TaaI ACNGT 6 cut(s) 464, 592, 877, 1216, 1759, 1882
TaqI TCGA 3 cut(s) 681, 966, 1500
TaqII GACCGA 1 cut(s) 706
TatI WGTACW 2 cut(s) 1585, 1652
TauI GCSGC 3 cut(s) 45, 158, 552
TfiI GAWTC 6 cut(s) 217, 336, 347, 506, 1169, 1270
Tru1I TTAA 6 cut(s) 144, 957, 1194, 1692, 1797, 2040
Tru9I TTAA 6 cut(s) 144, 957, 1194, 1692, 1797, 2040
TscAI CASTG 9 cut(s) 89, 219, 367, 469, 490, 535, 736, 1434, 1642
TseFI GTSAC 5 cut(s) 731, 802, 1520, 1640, 1759
TseI GCWGC 8 cut(s) 542, 674, 881, 1010, 1208, 1415, 1912, 2000
Tsp45I GTSAC 5 cut(s) 731, 802, 1520, 1640, 1759
TspDTI ATGAA 6 cut(s) 186, 253, 803, 1161, 1262, 1506
TspGWI ACGGA 2 cut(s) 528, 1316
TspMI CCCGGG 1 cut(s) 1550
TspRI CASTG 9 cut(s) 89, 219, 367, 469, 490, 535, 736, 1434, 1642
VpaK11BI GGWCC 3 cut(s) 1349, 1477, 1895
VspI ATTAAT 1 cut(s) 957
XapI RAATTY 3 cut(s) 902, 1072, 1502
XbaI TCTAGA 2 cut(s) 325, 1343
XceI RCATGY 1 cut(s) 1574
XcmI CCANNNNNNNNNTGG 2 cut(s) 390, 1680
XmaI CCCGGG 1 cut(s) 1550
XmiI GTMKAC 3 cut(s) 29, 627, 1545
XmnI GAANNNNTTC 1 cut(s) 1874
XspI CTAG 6 cut(s) 326, 615, 1139, 1344, 1712, 1752
ZrmI AGTACT 1 cut(s) 1654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.