Rh7AG180400

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012611
Physical Location & Seq
Forward (+)
16063964 .. 16064837
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG180400.1

Sequence Viewer

Length: 438 bp
ATGGCCAAGAGAGGAAGGTTCACATTGTATACATGGGAGAGCGGCACCATGGAGATTTTTCTGCTCAATCCACACACCATTCAGTGCTACAAAGCCTTCAAGTTGGCCAAAGAGTCGCTGATCTATAGTTATGGGAAGAGATTTAATGGGTTTGCCGCAAAGTTGTTGGATGAAGAAGTTGCAGAGTTGGCAGAAATGGAGGGTATAGTTTCAGTGATTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACGTGGAGCATCTGTAGGAGGAGAGGTTGTGATAGGGCTTCTAGACTCAGGAATCTGGCCGGAATCTGATAGCTTCAGTGATGATGGCTTTGGCCCTCCACCTAAGAAATGGAAAGGCAAATGCAAAGGTGCCAACTTCACCTGCAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

145

Amino Acids

16.22

Weight (kDa)

8.97

Isoelectric Point (pI)

16.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 81 2.5e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 2 cut(s) 44, 413
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 29
AciI CCGC 2 cut(s) 42, 156
AcoI YGGCCR 3 cut(s) 3, 105, 341
AcuI CTGAAG 1 cut(s) 343
AgsI TTSAA 1 cut(s) 100
AluBI AGCT 2 cut(s) 238, 357
AluI AGCT 2 cut(s) 238, 357
AoxI GGCC 5 cut(s) 3, 105, 280, 341, 376
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 415
BalI TGGCCA 2 cut(s) 5, 107
BanI GGYRCC 2 cut(s) 44, 413
BccI CCATC 1 cut(s) 362
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 124, 297
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 2 cut(s) 43, 156
BlsI GCNGC 2 cut(s) 44, 157
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 2 cut(s) 46, 415
BmsI GCATC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 6 cut(s) 282, 294, 297, 312, 324, 327
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 318
BshFI GGCC 5 cut(s) 5, 107, 282, 343, 378
BshNI GGYRCC 2 cut(s) 44, 413
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 5 cut(s) 5, 107, 282, 343, 378
Bsp143I GATC 1 cut(s) 120
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 2 cut(s) 42, 156
BspANI GGCC 5 cut(s) 5, 107, 282, 343, 378
BspCNI CTCAG 1 cut(s) 344
BspLI GGNNCC 2 cut(s) 46, 415
BspMI ACCTGC 1 cut(s) 434
BspT107I GGYRCC 2 cut(s) 44, 413
BsrBI CCGCTC 1 cut(s) 42
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 1 cut(s) 120
BssNAI GTATAC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 48
Bst1107I GTATAC 1 cut(s) 30
Bst6I CTCTTC 1 cut(s) 131
BstDEI CTNAG 2 cut(s) 331, 387
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstSFI CTRYAG 2 cut(s) 124, 297
BstZ17I GTATAC 1 cut(s) 30
BsuRI GGCC 5 cut(s) 5, 107, 282, 343, 378
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 3 cut(s) 89, 219, 367
BveI ACCTGC 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 377
CviAII CATG 3 cut(s) 33, 49, 265
DdeI CTNAG 2 cut(s) 331, 387
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
EaeI YGGCCR 3 cut(s) 3, 105, 341
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 3 cut(s) 36, 52, 268
FaiI YATR 7 cut(s) 30, 34, 50, 126, 132, 206, 266
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 32, 48, 264
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 43, 156
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 43, 156
FspBI CTAG 1 cut(s) 326
GluI GCNGC 2 cut(s) 43, 156
HaeIII GGCC 5 cut(s) 5, 107, 282, 343, 378
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 36, 52, 268
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 5 cut(s) 113, 217, 329, 336, 347
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 2 cut(s) 21, 30
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 2 cut(s) 326, 333
Hpy8I GTNNAC 2 cut(s) 21, 30
HpyAV CCTTC 2 cut(s) 9, 106
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 3 cut(s) 182, 408, 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 2 cut(s) 331, 387
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 3 cut(s) 36, 52, 268
Kzo9I GATC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 3 cut(s) 318, 325, 357
LweI GCATC 1 cut(s) 302
MaeI CTAG 1 cut(s) 326
MaeII ACGT 1 cut(s) 286
MalI GATC 1 cut(s) 122
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 120
MboII GAAGA 2 cut(s) 148, 185
MlsI TGGCCA 2 cut(s) 5, 107
MluNI TGGCCA 2 cut(s) 5, 107
MlyI GAGTC 2 cut(s) 122, 323
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 5 cut(s) 5, 193, 296, 301, 390
Mox20I TGGCCA 2 cut(s) 5, 107
MscI TGGCCA 2 cut(s) 5, 107
MseI TTAA 1 cut(s) 144
Msp20I TGGCCA 2 cut(s) 5, 107
MspI CCGG 1 cut(s) 344
MwoI GCNNNNNNNGC 1 cut(s) 188
NcoI CCATGG 1 cut(s) 48
NdeII GATC 1 cut(s) 120
NlaIII CATG 3 cut(s) 36, 52, 268
NlaIV GGNNCC 2 cut(s) 46, 415
PaqCI CACCTGC 1 cut(s) 434
PfeI GAWTC 3 cut(s) 217, 336, 347
PkrI GCNGC 2 cut(s) 44, 157
PleI GAGTC 2 cut(s) 121, 323
PpsI GAGTC 2 cut(s) 121, 323
PspN4I GGNNCC 2 cut(s) 46, 415
PspPI GGNCC 1 cut(s) 377
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 43, 156
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 2 cut(s) 122, 323
SetI ASST 9 cut(s) 20, 240, 254, 289, 312, 359, 388, 415, 428
SfaNI GCATC 1 cut(s) 302
SfcI CTRYAG 2 cut(s) 124, 297
SsiI CCGC 2 cut(s) 42, 156
SspMI CTAG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 48
TaiI ACGT 1 cut(s) 289
TauI GCSGC 2 cut(s) 45, 158
TfiI GAWTC 3 cut(s) 217, 336, 347
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 3 cut(s) 89, 219, 367
TspDTI ATGAA 2 cut(s) 186, 253
TspRI CASTG 3 cut(s) 89, 219, 367
XbaI TCTAGA 1 cut(s) 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.