Rw7G015850

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012611
Physical Location & Seq
Forward (+)
16169983 .. 16173143
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015850.1

Sequence Viewer

Length: 2211 bp
ATGACTACTCATTTCCTTCTACACACATTATTTCTTACAGCATTTGCTCTATCTTGCCATGGCCAAGAGAGGAAGGTTCACATTGTATACATGGGAGAGCGGCACCATGGAGATTTTTCTGCTCAATCCACACACCATTCAGTGCTACAAAGCGTACTTGGAAGCTTCAAGTTGGCCAAAGAGTCGCTGATCTATAGTTATGGGAAGAGCTTTAATGGGTTTGCCGCAAAGTTGTCGGATGAAGAAGTTGCAGAGTTGGCAGAAATGGATGGTATAGTTTCAGTGATTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACGTGGAGCATCTGTAGGAGAAGAGGTTGTGATAGGGCTTCTAGACTCAGGAATCTGGCCGGAATCTGATAGCTTCAGTGATGATGGCTTTGGCCCTCCACCTAAGAAATGGAAAGGCAAATGCAAAGGTGCCAACTTCACCTGCAACAACAAAGTTATTGGAGCCCGCTACTACAACAGTGATGGCTCCTATGACCCCACTGACATCCAGTCCCCAAGAGATTCCAACGGACATGGAAGCCACACTGCTTCAACTGCAGCAGGCCGCGAGGTGCCAGCCAGTTACTTGGGGTTAGCTTCTGGAACTGCAAGAGGTGGAGTCCCGAATGCTAGGATTGCTGTCTACAAAGTTTGCTGGGCATCTGGATGTTATTCTTCAGATGTCCTTGCAGCATTCGATGATGCAATAGCAGATGGAGTTGATATTATATCAGTCTCACTTGGCAGTGACTTCCCACTCCAATACTTAGAAGACCCCATTGCTATTGGGTCTTTCCATGCCATGAAATATGGTATAGTGACCTCAACTTCTGCTGGAAACTCTGGCCCTGCTCCATTATCAGTTTCTAATTATGCACCATGGATAATGACCGTTGCTGCCAGCACCATTGACAGAAAATTTGTGGCAAAAGCAGTTCTTGGCAATGGAGAAGTCTATAGTGGACTTTCAATTAATAATTTCGACCTGAATGGAAAGTCATATCCCCTAATATGGGGAGGAGATGCTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGCAAGCTTTTTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGACGATGGCTCGGGTGTTCTACTAGCCAATGGTGTCGGTACAATTATGGCCGATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTACAATAGGAGATGGACAGAGAATTATGAACTATATTAGATCAACAGCGAATCCAAATGCAACAATTCTTGTTGGCGAGACGGACATGGATATCATGGCACCTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCTATAACCCCAGATATCCTCAAGCCTGATCTGACCGCCCCTGGTGTTGACATACTTGCTGCTTGGTCTGCAGTGGCACCACCTTCCATTTACGAAGATGACACCAGGAGTGTCGAATTTAATGTTATCTCCGGGACATCCATGTCCTGCCCTCATGCTAGTGGTGCTGCAGCTTATGTCAAGGCTGCACATCCAAATTGGTCTCCTGCTGCAATCAAATCTGCACTCATGACAACAGCTCATGTCCTGGATCCAAAGAAGCATGAAGACCTTGAATTCGCTTATGGGTCTGGTCACATCAACCCTCTGAAAGCAGTTGACCCCGGGCTTGTTTTTGATGCACATGTAGCAGACTATGTACATTTCCTCTGCAAGCAGGGTTACAATACCACCACTTTGAAGCTCATCACTGGTGACAACAGCAGTACTTGTGGCAGGACAAAGCCAGGAAGAGCTTGGGATCTTAACTATCCATCGTTCTCTCTAGCAGTTGAAGATGGCCAGCATATCAATGCTGTCTTTACTAGAACAGTCACAAATGTGGGATCACCAAACTCAACCTATACTATTAAGGCGCACACACCCTCCTCAAGTGTTTCGGTAAAACCATCAGCCATTTCTTTCTCTTCTGTCGGTGAGAAGAAATCCTTCACAGTCAAAGTAATTGGTCCGCCTATTGCACAGCAGCCGATCATGTCCGGTGCAATCGTGTTGACTGATGGGATCCATCTGGTGAGAAGCCCTTTCGTTATCTACACAATCCTCCCTGGTGCTGCACATTCGTACTCCATACCTGAGAAAAAAACCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

736

Amino Acids

78.03

Weight (kDa)

6.17

Isoelectric Point (pI)

25.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 26 - 104 7.8e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 124 - 563 2.9e-45 Subtilase family
fn3_6 PF17766 619 - 716 6.9e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 503
AasI GACNNNNNNGTC 1 cut(s) 1537
Acc36I ACCTGC 1 cut(s) 503
AccB1I GGYRCC 5 cut(s) 102, 482, 625, 1357, 1471
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 2 cut(s) 87, 696
AccII CGCG 1 cut(s) 621
AciI CCGC 6 cut(s) 100, 225, 520, 619, 1431, 2066
AclWI GGATC 6 cut(s) 1640, 1653, 1863, 1948, 2113, 2126
AcoI YGGCCR 5 cut(s) 61, 174, 410, 1203, 1894
AcsI RAATTY 3 cut(s) 971, 1511, 1670
AcuI CTGAAG 2 cut(s) 412, 714
AfaI GTAC 5 cut(s) 156, 1195, 1755, 1822, 2180
AfiI CCNNNNNNNGG 2 cut(s) 1065, 1066
AflIII ACRYGT 1 cut(s) 1738
AgsI TTSAA 6 cut(s) 169, 606, 1023, 1670, 1795, 1889
AhdI GACNNNNNGTC 1 cut(s) 562
AjnI CCWGG 5 cut(s) 1435, 1499, 1641, 1840, 2161
AleI CACNNNNGTG 1 cut(s) 1934
Alw26I GTCTC 3 cut(s) 793, 1331, 1602
AlwI GGATC 6 cut(s) 1640, 1653, 1863, 1948, 2113, 2126
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 2 cut(s) 1165, 1718
AoxI GGCC 9 cut(s) 61, 174, 349, 410, 445, 616, 898, 1203, 1894
ApoI RAATTY 3 cut(s) 971, 1511, 1670
ArsI GACNNNNNNTTYG 4 cut(s) 956, 988, 1655, 1687
AseI ATTAAT 1 cut(s) 1026
Asp700I GAANNNNTTC 1 cut(s) 2042
AspLEI GCGC 1 cut(s) 1972
AspS9I GGNCC 4 cut(s) 446, 899, 1388, 2063
AsuC2I CCSGG 3 cut(s) 1528, 1719, 1720
AsuHPI GGTGA 5 cut(s) 484, 1820, 1935, 2042, 2140
AvaI CYCGRG 2 cut(s) 1165, 1718
AvaII GGWCC 2 cut(s) 1388, 2063
BalI TGGCCA 3 cut(s) 63, 176, 1896
BamHI GGATCC 2 cut(s) 1645, 2118
BanI GGYRCC 5 cut(s) 102, 482, 625, 1357, 1471
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 2 cut(s) 831, 1668
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 5 cut(s) 1437, 1501, 1643, 1842, 2163
BcnI CCSGG 3 cut(s) 1528, 1719, 1720
BcoDI GTCTC 3 cut(s) 793, 1331, 1602
BfaI CTAG 7 cut(s) 395, 684, 1178, 1383, 1554, 1880, 1920
BfmI CTRYAG 6 cut(s) 193, 366, 609, 1009, 1464, 1563
BfuAI ACCTGC 1 cut(s) 503
BmcAI AGTACT 1 cut(s) 1822
Bme1390I CCNGG 8 cut(s) 1437, 1501, 1528, 1643, 1719, 1720, 1842, 2163
Bme18I GGWCC 2 cut(s) 1388, 2063
BmeRI GACNNNNNGTC 1 cut(s) 562
BmeT110I CYCGRG 2 cut(s) 1165, 1718
BmgT120I GGNCC 4 cut(s) 446, 899, 1388, 2063
BmrFI CCNGG 8 cut(s) 1437, 1501, 1528, 1643, 1719, 1720, 1842, 2163
BmsI GCATC 5 cut(s) 371, 722, 745, 1066, 1723
BpiI GAAGAC 2 cut(s) 831, 1668
BpmI CTGGAG 1 cut(s) 1116
BpuEI CTTGAG 2 cut(s) 1400, 1969
BpuMI CCSGG 3 cut(s) 1528, 1719, 1720
BsaI GGTCTC 1 cut(s) 1602
BsaJI CCNNGG 7 cut(s) 58, 106, 932, 1435, 1717, 1718, 2161
BsaWI WCCGGW 1 cut(s) 2093
BsaXI ACNNNNNCTCC 6 cut(s) 351, 363, 381, 393, 1964, 1994
Bsc4I CCNNNNNNNGG 2 cut(s) 1065, 1066
Bse1I ACTGG 3 cut(s) 562, 633, 1810
Bse3DI GCAATG 2 cut(s) 831, 1003
BseBI CCWGG 5 cut(s) 1437, 1501, 1643, 1842, 2163
BseDI CCNNGG 7 cut(s) 58, 106, 932, 1435, 1717, 1718, 2161
BseGI GGATG 6 cut(s) 244, 274, 558, 725, 1532, 1585
BseLI CCNNNNNNNGG 2 cut(s) 1065, 1066
BseMI GCAATG 2 cut(s) 831, 1003
BseMII CTCAG 2 cut(s) 414, 2181
BseNI ACTGG 3 cut(s) 562, 633, 1810
BseRI GAGGAG 2 cut(s) 1086, 1972
BseYI CCCAGC 1 cut(s) 708
BsgI GTGCAG 3 cut(s) 1566, 1602, 2154
Bsh1236I CGCG 1 cut(s) 621
BshFI GGCC 9 cut(s) 63, 176, 351, 412, 447, 618, 900, 1205, 1896
BshNI GGYRCC 5 cut(s) 102, 482, 625, 1357, 1471
BsiHKCI CYCGRG 2 cut(s) 1165, 1718
BsiSI CCGG 4 cut(s) 413, 1527, 1719, 2094
BslFI GGGAC 4 cut(s) 341, 550, 659, 1543
BslI CCNNNNNNNGG 2 cut(s) 1065, 1066
BsmAI GTCTC 3 cut(s) 793, 1331, 1602
BsmBI CGTCTC 1 cut(s) 1331
BsmFI GGGAC 4 cut(s) 341, 550, 659, 1543
BsmI GAATGC 2 cut(s) 685, 746
BsnI GGCC 9 cut(s) 63, 176, 351, 412, 447, 618, 900, 1205, 1896
Bso31I GGTCTC 1 cut(s) 1602
BsoBI CYCGRG 2 cut(s) 1165, 1718
Bsp1286I GDGCHC 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 1753
Bsp143I GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
Bsp19I CCATGG 3 cut(s) 58, 106, 932
BspACI CCGC 6 cut(s) 100, 225, 520, 619, 1431, 2066
BspANI GGCC 9 cut(s) 63, 176, 351, 412, 447, 618, 900, 1205, 1896
BspCNI CTCAG 2 cut(s) 413, 2182
BspFNI CGCG 1 cut(s) 621
BspHI TCATGA 1 cut(s) 1623
BspMAI CTGCAG 3 cut(s) 613, 1468, 1567
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 6 cut(s) 1640, 1653, 1863, 1948, 2113, 2126
BspQI GCTCTTC 2 cut(s) 200, 1840
BspT107I GGYRCC 5 cut(s) 102, 482, 625, 1357, 1471
BspTNI GGTCTC 1 cut(s) 1602
BsrBI CCGCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 831, 1003
BsrGI TGTACA 1 cut(s) 1753
BsrI ACTGG 3 cut(s) 562, 633, 1810
BssECI CCNNGG 7 cut(s) 58, 106, 932, 1435, 1717, 1718, 2161
BssMI GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
BssNAI GTATAC 1 cut(s) 88
BssT1I CCWWGG 3 cut(s) 58, 106, 932
Bst1107I GTATAC 1 cut(s) 88
Bst2UI CCWGG 5 cut(s) 1437, 1501, 1643, 1842, 2163
Bst4CI ACNGT 5 cut(s) 533, 946, 1255, 1927, 2050
Bst6I CTCTTC 4 cut(s) 200, 369, 1840, 2026
BstAUI TGTACA 1 cut(s) 1753
BstC8I GCNNGC 7 cut(s) 520, 616, 630, 955, 1117, 1769, 1898
BstDEI CTNAG 4 cut(s) 400, 456, 820, 2190
BstDSI CCRYGG 3 cut(s) 58, 106, 932
BstF5I GGATG 6 cut(s) 244, 274, 558, 725, 1532, 1585
BstFNI CGCG 1 cut(s) 621
BstHHI GCGC 1 cut(s) 1972
BstKTI GATC 8 cut(s) 192, 1301, 1426, 1648, 1858, 1943, 2088, 2121
BstMAI GTCTC 3 cut(s) 793, 1331, 1602
BstMBI GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
BstMWI GCNNNNNNNGC 6 cut(s) 257, 608, 689, 1463, 1559, 1742
BstNI CCWGG 5 cut(s) 1437, 1501, 1643, 1842, 2163
BstNSI RCATGY 1 cut(s) 1742
BstSCI CCNGG 8 cut(s) 1435, 1499, 1526, 1641, 1717, 1718, 1840, 2161
BstSFI CTRYAG 6 cut(s) 193, 366, 609, 1009, 1464, 1563
BstUI CGCG 1 cut(s) 621
BstV2I GAAGAC 2 cut(s) 831, 1668
BstX2I RGATCY 3 cut(s) 1645, 1855, 2118
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 3 cut(s) 1645, 1855, 2118
BstZ17I GTATAC 1 cut(s) 88
BsuRI GGCC 9 cut(s) 63, 176, 351, 412, 447, 618, 900, 1205, 1896
BtgI CCRYGG 3 cut(s) 58, 106, 932
BtsCI GGATG 6 cut(s) 244, 274, 558, 725, 1532, 1585
BtsI GCAGTG 3 cut(s) 597, 805, 1473
BtsIMutI CAGTG 9 cut(s) 147, 288, 436, 538, 552, 597, 805, 1473, 1803
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 7 cut(s) 520, 616, 630, 955, 1117, 1769, 1898
CaiI CAGNNNCTG 1 cut(s) 1253
CciI TCATGA 1 cut(s) 1623
CfoI GCGC 1 cut(s) 1972
Cfr13I GGNCC 4 cut(s) 446, 899, 1388, 2063
Cfr9I CCCGGG 1 cut(s) 1718
Csp6I GTAC 5 cut(s) 155, 1194, 1754, 1821, 2179
CviQI GTAC 5 cut(s) 155, 1194, 1754, 1821, 2179
DdeI CTNAG 4 cut(s) 400, 456, 820, 2190
DpnI GATC 8 cut(s) 191, 1300, 1425, 1647, 1857, 1942, 2087, 2120
DpnII GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
DraI TTTAAA 1 cut(s) 1125
DrdI GACNNNNNNGTC 1 cut(s) 1537
DriI GACNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 1537
EaeI YGGCCR 5 cut(s) 61, 174, 410, 1203, 1894
Eam1104I CTCTTC 4 cut(s) 200, 369, 1840, 2026
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 4 cut(s) 200, 369, 1840, 2026
EciI GGCGGA 1 cut(s) 2055
Eco130I CCWWGG 3 cut(s) 58, 106, 932
Eco24I GRGCYC 1 cut(s) 520
Eco31I GGTCTC 1 cut(s) 1602
Eco32I GATATC 2 cut(s) 1351, 1411
Eco47I GGWCC 2 cut(s) 1388, 2063
Eco57I CTGAAG 2 cut(s) 412, 714
Eco88I CYCGRG 2 cut(s) 1165, 1718
EcoO109I RGGNCCY 1 cut(s) 1388
EcoRI GAATTC 1 cut(s) 1670
EcoRII CCWGG 5 cut(s) 1435, 1499, 1641, 1840, 2161
EcoRV GATATC 2 cut(s) 1351, 1411
EcoT14I CCWWGG 3 cut(s) 58, 106, 932
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 3 cut(s) 58, 106, 932
Esp3I CGTCTC 1 cut(s) 1331
FalI AAGNNNNNCTT 4 cut(s) 975, 1007, 2027, 2059
FaqI GGGAC 4 cut(s) 341, 550, 659, 1543
FauI CCCGC 1 cut(s) 527
FblI GTMKAC 2 cut(s) 87, 696
FokI GGATG 6 cut(s) 251, 281, 545, 732, 1519, 1572
FriOI GRGCYC 1 cut(s) 520
FspBI CTAG 7 cut(s) 395, 684, 1178, 1383, 1554, 1880, 1920
GlaI GCGC 1 cut(s) 1971
GsaI CCCAGC 1 cut(s) 712
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 9 cut(s) 63, 176, 351, 412, 447, 618, 900, 1205, 1896
HapII CCGG 4 cut(s) 413, 1527, 1719, 2094
HhaI GCGC 1 cut(s) 1972
Hin6I GCGC 1 cut(s) 1970
HinP1I GCGC 1 cut(s) 1970
HincII GTYRAC 3 cut(s) 1444, 1714, 2109
HindII GTYRAC 3 cut(s) 1444, 1714, 2109
HindIII AAGCTT 3 cut(s) 163, 305, 1117
HinfI GANTC 9 cut(s) 182, 286, 398, 405, 416, 575, 672, 1208, 1309
HpaII CCGG 4 cut(s) 413, 1527, 1719, 2094
HphI GGTGA 5 cut(s) 484, 1820, 1935, 2042, 2140
Hpy166II GTNNAC 7 cut(s) 79, 88, 697, 1016, 1444, 1714, 2109
Hpy188I TCNGA 7 cut(s) 238, 421, 733, 1108, 1222, 1428, 1704
Hpy188III TCNNGA 7 cut(s) 395, 402, 654, 676, 717, 1383, 1624
Hpy8I GTNNAC 7 cut(s) 79, 88, 697, 1016, 1444, 1714, 2109
HpyAV CCTTC 4 cut(s) 26, 67, 1488, 2053
HpyCH4III ACNGT 5 cut(s) 533, 946, 1255, 1927, 2050
HpyCH4IV ACGT 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 608, 689, 1463, 1559, 1742
HpyF3I CTNAG 4 cut(s) 400, 456, 820, 2190
HpySE526I ACGT 1 cut(s) 355
HspAI GCGC 1 cut(s) 1970
Kzo9I GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
LguI GCTCTTC 2 cut(s) 200, 1840
LmnI GCTCC 5 cut(s) 359, 515, 545, 910, 1097
LweI GCATC 5 cut(s) 371, 722, 745, 1066, 1723
MaeI CTAG 7 cut(s) 395, 684, 1178, 1383, 1554, 1880, 1920
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 8 cut(s) 635, 800, 871, 1258, 1688, 1775, 1808, 1927
MalI GATC 8 cut(s) 191, 1300, 1425, 1647, 1857, 1942, 2087, 2120
MbiI CCGCTC 1 cut(s) 100
MboI GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
MflI RGATCY 3 cut(s) 1645, 1855, 2118
MhlI GDGCHC 1 cut(s) 520
MlsI TGGCCA 3 cut(s) 63, 176, 1896
MluNI TGGCCA 3 cut(s) 63, 176, 1896
MlyI GAGTC 3 cut(s) 191, 392, 681
MmeI TCCRAC 2 cut(s) 216, 603
Mox20I TGGCCA 3 cut(s) 63, 176, 1896
MroXI GAANNNNTTC 1 cut(s) 2042
MscI TGGCCA 3 cut(s) 63, 176, 1896
MseI TTAA 8 cut(s) 213, 1026, 1124, 1233, 1515, 1860, 1965, 2209
MslI CAYNNNNRTG 4 cut(s) 718, 1554, 1905, 1934
Msp20I TGGCCA 3 cut(s) 63, 176, 1896
MspI CCGG 4 cut(s) 413, 1527, 1719, 2094
MspR9I CCNGG 8 cut(s) 1437, 1501, 1528, 1643, 1719, 1720, 1842, 2163
Mva1269I GAATGC 2 cut(s) 685, 746
MvaI CCWGG 5 cut(s) 1437, 1501, 1643, 1842, 2163
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 6 cut(s) 257, 608, 689, 1463, 1559, 1742
NciI CCSGG 3 cut(s) 1528, 1719, 1720
NcoI CCATGG 3 cut(s) 58, 106, 932
NdeII GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
NmuCI GTSAC 5 cut(s) 800, 871, 1688, 1808, 1927
NspI RCATGY 1 cut(s) 1742
OliI CACNNNNGTG 1 cut(s) 1934
PagI TCATGA 1 cut(s) 1623
PaqCI CACCTGC 1 cut(s) 503
PciI ACATGT 1 cut(s) 1738
PciSI GCTCTTC 2 cut(s) 200, 1840
PctI GAATGC 2 cut(s) 685, 746
PdmI GAANNNNTTC 1 cut(s) 2042
PfeI GAWTC 6 cut(s) 286, 405, 416, 575, 1208, 1309
PfoI TCCNGGA 2 cut(s) 1526, 1641
PleI GAGTC 3 cut(s) 190, 392, 680
PpsI GAGTC 3 cut(s) 190, 392, 680
PpuMI RGGWCCY 1 cut(s) 1388
PscI ACATGT 1 cut(s) 1738
PshBI ATTAAT 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 1388
Psp6I CCWGG 5 cut(s) 1435, 1499, 1641, 1840, 2161
PspFI CCCAGC 1 cut(s) 708
PspGI CCWGG 5 cut(s) 1435, 1499, 1641, 1840, 2161
PspPI GGNCC 4 cut(s) 446, 899, 1388, 2063
PspPPI RGGWCCY 1 cut(s) 1388
PstI CTGCAG 3 cut(s) 613, 1468, 1567
PstNI CAGNNNCTG 1 cut(s) 1253
PsuI RGATCY 3 cut(s) 1645, 1855, 2118
RsaI GTAC 5 cut(s) 156, 1195, 1755, 1822, 2180
RsaNI GTAC 5 cut(s) 155, 1194, 1754, 1821, 2179
RseI CAYNNNNRTG 4 cut(s) 718, 1554, 1905, 1934
SapI GCTCTTC 2 cut(s) 200, 1840
SaqAI TTAA 8 cut(s) 213, 1026, 1124, 1233, 1515, 1860, 1965, 2209
Sau3AI GATC 8 cut(s) 189, 1298, 1423, 1645, 1855, 1940, 2085, 2118
Sau96I GGNCC 4 cut(s) 446, 899, 1388, 2063
ScaI AGTACT 1 cut(s) 1822
SchI GAGTC 3 cut(s) 191, 392, 681
ScrFI CCNGG 8 cut(s) 1437, 1501, 1528, 1643, 1719, 1720, 1842, 2163
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 5 cut(s) 371, 722, 745, 1066, 1723
SfcI CTRYAG 6 cut(s) 193, 366, 609, 1009, 1464, 1563
SinI GGWCC 2 cut(s) 1388, 2063
SmaI CCCGGG 1 cut(s) 1720
SmiMI CAYNNNNRTG 4 cut(s) 718, 1554, 1905, 1934
SmlI CTYRAG 2 cut(s) 1415, 1984
SmoI CTYRAG 2 cut(s) 1415, 1984
SsiI CCGC 6 cut(s) 100, 225, 520, 619, 1431, 2066
SspMI CTAG 7 cut(s) 395, 684, 1178, 1383, 1554, 1880, 1920
StyD4I CCNGG 8 cut(s) 1435, 1499, 1526, 1641, 1717, 1718, 1840, 2161
StyI CCWWGG 3 cut(s) 58, 106, 932
TaaI ACNGT 5 cut(s) 533, 946, 1255, 1927, 2050
TaiI ACGT 1 cut(s) 358
TaqI TCGA 3 cut(s) 750, 1035, 1509
TatI WGTACW 2 cut(s) 1753, 1820
TauI GCSGC 3 cut(s) 103, 227, 621
TfiI GAWTC 6 cut(s) 286, 405, 416, 575, 1208, 1309
Tru1I TTAA 8 cut(s) 213, 1026, 1124, 1233, 1515, 1860, 1965, 2209
Tru9I TTAA 8 cut(s) 213, 1026, 1124, 1233, 1515, 1860, 1965, 2209
TscAI CASTG 9 cut(s) 147, 288, 436, 538, 559, 604, 805, 1473, 1810
TseFI GTSAC 5 cut(s) 800, 871, 1688, 1808, 1927
Tsp45I GTSAC 5 cut(s) 800, 871, 1688, 1808, 1927
TspDTI ATGAA 6 cut(s) 255, 322, 872, 1200, 1301, 1674
TspGWI ACGGA 2 cut(s) 597, 1355
TspMI CCCGGG 1 cut(s) 1718
TspRI CASTG 9 cut(s) 147, 288, 436, 538, 559, 604, 805, 1473, 1810
VpaK11BI GGWCC 2 cut(s) 1388, 2063
VspI ATTAAT 1 cut(s) 1026
XapI RAATTY 3 cut(s) 971, 1511, 1670
XbaI TCTAGA 2 cut(s) 394, 1382
XceI RCATGY 1 cut(s) 1742
XcmI CCANNNNNNNNNTGG 2 cut(s) 459, 1848
XmaI CCCGGG 1 cut(s) 1718
XmiI GTMKAC 2 cut(s) 87, 696
XmnI GAANNNNTTC 1 cut(s) 2042
XspI CTAG 7 cut(s) 395, 684, 1178, 1383, 1554, 1880, 1920
ZrmI AGTACT 1 cut(s) 1822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.