Rh7DG183500

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012611
Physical Location & Seq
Forward (+)
17042384 .. 17043891
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG183500.1

Sequence Viewer

Length: 978 bp
ATGCAGGGACTTTCAATTAATAATTTCGACCTGAATGGAAAGTCATATCCCCTAATATGGGGAGGAGATGCTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGCAAGCTTTTGTCCAAATGGAGCCCTGAATTCACTTAAAGTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGACGATGGCTCGGGTGTTCTACTAGCCAATGGTGTCGGTACAATTATGGCCGATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTACAATAGGAGATGGACAGAGAATTATGAACTATATTAGATCAACAGCGAATCCTAATGCAACAATTCTTGTTGGCGAGACGGACATGGATATCATGGCACCTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCTATAACCCCAGATATCCTCAAGCCTGATCTGACCGCCCCTGGTGTTGACATACTTGCTGCTTGGTCTCCAGTGGCACCACCTTCCATTTACGAAGATGACACCAGGAGTGTCGAATTTAATGGTTACAATACCACCACTTTGAAGCTCATCACTGGTGACAACAGCAGTACTTGTGGCAGGACAAAGCCAGGAAGAGCTTGGGATCTTAACTATCCATCGTTCTCTCTAGCAGTTGAAGATGGCCAGCATGTCAATGCTGTCTTTACTAGAACAGTCACAAATGTGGGATCACCAAACTCAACCTATACTATTAAGGCGCACACACCCTCCTCAAGTGTTTCGGTAAAACCATCAACCATTTCTTTCTCTTCTGTCGGTGAGAAGAAATCCTTCACAGTCAAAGTAATTGGTCCGCCTATTGCACAGCAGCCGATCATGTCCGGTGCAATCGTGTTGACCGATGGGATCCATCTGGTGAGAAGCCCTCTCGTTATCTACACAATCCTCCCTGGTGCTGCACATTCGTACTCCATACCTGAGAAAAAAACCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

325

Amino Acids

34.19

Weight (kDa)

5.8

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 208 - 305 3.7e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 379, 493
AciI CCGC 2 cut(s) 453, 833
AclWI GGATC 4 cut(s) 630, 715, 880, 893
AcoI YGGCCR 2 cut(s) 225, 661
AcsI RAATTY 2 cut(s) 133, 533
AfaI GTAC 3 cut(s) 217, 589, 947
AfiI CCNNNNNNNGG 2 cut(s) 57, 58
AgsI TTSAA 3 cut(s) 15, 562, 656
AjnI CCWGG 4 cut(s) 457, 521, 607, 928
AleI CACNNNNGTG 1 cut(s) 701
AluBI AGCT 4 cut(s) 92, 111, 565, 617
AluI AGCT 4 cut(s) 92, 111, 565, 617
Alw26I GTCTC 2 cut(s) 353, 489
AlwI GGATC 4 cut(s) 630, 715, 880, 893
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 2 cut(s) 225, 661
ApeKI GCWGC 5 cut(s) 71, 269, 476, 847, 935
ApoI RAATTY 2 cut(s) 133, 533
AseI ATTAAT 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 809
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 2 cut(s) 410, 830
AsuHPI GGTGA 4 cut(s) 587, 702, 809, 907
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 2 cut(s) 410, 830
BalI TGGCCA 1 cut(s) 663
BamHI GGATCC 1 cut(s) 885
BanI GGYRCC 2 cut(s) 379, 493
BanII GRGCYC 1 cut(s) 130
BbvI GCAGC 5 cut(s) 58, 281, 463, 859, 922
BccI CCATC 7 cut(s) 176, 287, 643, 653, 778, 875, 897
BciT130I CCWGG 4 cut(s) 459, 523, 609, 930
BcoDI GTCTC 2 cut(s) 353, 489
BfaI CTAG 4 cut(s) 200, 405, 647, 687
BisI GCNGC 5 cut(s) 72, 270, 477, 848, 936
BlsI GCNGC 5 cut(s) 73, 271, 478, 849, 937
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 4 cut(s) 459, 523, 609, 930
Bme18I GGWCC 2 cut(s) 410, 830
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 410, 830
BmiI GGNNCC 5 cut(s) 127, 381, 412, 495, 887
BmrFI CCNGG 4 cut(s) 459, 523, 609, 930
BmsI GCATC 1 cut(s) 58
BplI GAGNNNNNCTC 2 cut(s) 889, 921
BpmI CTGGAG 2 cut(s) 108, 471
BpuEI CTTGAG 2 cut(s) 422, 736
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 457, 928
BsaWI WCCGGW 1 cut(s) 860
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 58
Bse1I ACTGG 2 cut(s) 488, 577
BseBI CCWGG 4 cut(s) 459, 523, 609, 930
BseDI CCNNGG 2 cut(s) 457, 928
BseLI CCNNNNNNNGG 2 cut(s) 57, 58
BseMII CTCAG 1 cut(s) 948
BseNI ACTGG 2 cut(s) 488, 577
BseRI GAGGAG 2 cut(s) 78, 739
BseXI GCAGC 5 cut(s) 58, 281, 463, 859, 922
BsgI GTGCAG 1 cut(s) 921
BshFI GGCC 2 cut(s) 227, 663
BshNI GGYRCC 2 cut(s) 379, 493
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 1 cut(s) 861
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 57, 58
BsmAI GTCTC 2 cut(s) 353, 489
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 2 cut(s) 227, 663
Bso31I GGTCTC 1 cut(s) 489
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 6 cut(s) 320, 445, 622, 707, 852, 885
BspACI CCGC 2 cut(s) 453, 833
BspANI GGCC 2 cut(s) 227, 663
BspCNI CTCAG 1 cut(s) 949
BspLI GGNNCC 5 cut(s) 127, 381, 412, 495, 887
BspPI GGATC 4 cut(s) 630, 715, 880, 893
BspQI GCTCTTC 1 cut(s) 607
BspT107I GGYRCC 2 cut(s) 379, 493
BspTNI GGTCTC 1 cut(s) 489
BsrI ACTGG 2 cut(s) 488, 577
BssECI CCNNGG 2 cut(s) 457, 928
BssMI GATC 6 cut(s) 320, 445, 622, 707, 852, 885
Bst2UI CCWGG 4 cut(s) 459, 523, 609, 930
Bst4CI ACNGT 3 cut(s) 277, 694, 817
Bst6I CTCTTC 2 cut(s) 607, 793
BstC8I GCNNGC 2 cut(s) 109, 665
BstDEI CTNAG 1 cut(s) 957
BstHHI GCGC 1 cut(s) 739
BstKTI GATC 6 cut(s) 323, 448, 625, 710, 855, 888
BstMAI GTCTC 2 cut(s) 353, 489
BstMBI GATC 6 cut(s) 320, 445, 622, 707, 852, 885
BstNI CCWGG 4 cut(s) 459, 523, 609, 930
BstNSI RCATGY 1 cut(s) 671
BstSCI CCNGG 4 cut(s) 457, 521, 607, 928
BstV1I GCAGC 5 cut(s) 58, 281, 463, 859, 922
BstX2I RGATCY 2 cut(s) 622, 885
BstYI RGATCY 2 cut(s) 622, 885
BsuRI GGCC 2 cut(s) 227, 663
BtsIMutI CAGTG 2 cut(s) 495, 570
Cac8I GCNNGC 2 cut(s) 109, 665
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 1 cut(s) 739
Cfr13I GGNCC 2 cut(s) 410, 830
Csp6I GTAC 3 cut(s) 216, 588, 946
CviAII CATG 4 cut(s) 367, 376, 668, 856
CviQI GTAC 3 cut(s) 216, 588, 946
DdeI CTNAG 1 cut(s) 957
DpnI GATC 6 cut(s) 322, 447, 624, 709, 854, 887
DpnII GATC 6 cut(s) 320, 445, 622, 707, 852, 885
EaeI YGGCCR 2 cut(s) 225, 661
Eam1104I CTCTTC 2 cut(s) 607, 793
EarI CTCTTC 2 cut(s) 607, 793
EciI GGCGGA 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 130
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 2 cut(s) 373, 433
Eco47I GGWCC 2 cut(s) 410, 830
Eco88I CYCGRG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 4 cut(s) 457, 521, 607, 928
EcoRV GATATC 2 cut(s) 373, 433
EcoT38I GRGCYC 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 4 cut(s) 370, 379, 671, 859
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 21
FatI CATG 4 cut(s) 366, 375, 667, 855
Fnu4HI GCNGC 5 cut(s) 72, 270, 477, 848, 936
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 5 cut(s) 72, 270, 477, 848, 936
FspBI CTAG 4 cut(s) 200, 405, 647, 687
GlaI GCGC 1 cut(s) 738
GluI GCNGC 5 cut(s) 72, 270, 477, 848, 936
GsuI CTGGAG 2 cut(s) 108, 471
HaeIII GGCC 2 cut(s) 227, 663
HapII CCGG 1 cut(s) 861
HhaI GCGC 1 cut(s) 739
Hin1II CATG 4 cut(s) 370, 379, 671, 859
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HincII GTYRAC 2 cut(s) 466, 876
HindII GTYRAC 2 cut(s) 466, 876
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 2 cut(s) 230, 331
HpaII CCGG 1 cut(s) 861
HphI GGTGA 4 cut(s) 587, 702, 809, 907
Hpy166II GTNNAC 2 cut(s) 466, 876
Hpy188I TCNGA 3 cut(s) 100, 244, 450
Hpy188III TCNNGA 1 cut(s) 405
Hpy8I GTNNAC 2 cut(s) 466, 876
HpyAV CCTTC 2 cut(s) 510, 820
HpyCH4III ACNGT 3 cut(s) 277, 694, 817
HpyCH4V TGCA 6 cut(s) 4, 107, 341, 842, 866, 938
HpyF3I CTNAG 1 cut(s) 957
Hsp92II CATG 4 cut(s) 370, 379, 671, 859
HspAI GCGC 1 cut(s) 737
Kzo9I GATC 6 cut(s) 320, 445, 622, 707, 852, 885
LguI GCTCTTC 1 cut(s) 607
LmnI GCTCC 2 cut(s) 89, 125
Lsp1109I GCAGC 5 cut(s) 58, 281, 463, 859, 922
LweI GCATC 1 cut(s) 58
MaeI CTAG 4 cut(s) 200, 405, 647, 687
MaeIII GTNAC 4 cut(s) 280, 542, 575, 694
MalI GATC 6 cut(s) 322, 447, 624, 709, 854, 887
MboI GATC 6 cut(s) 320, 445, 622, 707, 852, 885
MboII GAAGA 7 cut(s) 228, 393, 524, 624, 668, 780, 814
MflI RGATCY 2 cut(s) 622, 885
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 663
MluNI TGGCCA 1 cut(s) 663
MnlI CCTC 8 cut(s) 56, 250, 401, 446, 757, 760, 915, 935
Mox20I TGGCCA 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 809
MscI TGGCCA 1 cut(s) 663
MseI TTAA 7 cut(s) 18, 141, 255, 537, 627, 732, 976
MslI CAYNNNNRTG 2 cut(s) 672, 701
Msp20I TGGCCA 1 cut(s) 663
MspI CCGG 1 cut(s) 861
MspR9I CCNGG 4 cut(s) 459, 523, 609, 930
MvaI CCWGG 4 cut(s) 459, 523, 609, 930
NdeII GATC 6 cut(s) 320, 445, 622, 707, 852, 885
NlaIII CATG 4 cut(s) 370, 379, 671, 859
NlaIV GGNNCC 5 cut(s) 127, 381, 412, 495, 887
NmuCI GTSAC 2 cut(s) 575, 694
NspI RCATGY 1 cut(s) 671
OliI CACNNNNGTG 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 2 cut(s) 230, 331
PkrI GCNGC 5 cut(s) 73, 271, 478, 849, 937
PpuMI RGGWCCY 1 cut(s) 410
PshBI ATTAAT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 4 cut(s) 457, 521, 607, 928
PspGI CCWGG 4 cut(s) 457, 521, 607, 928
PspN4I GGNNCC 5 cut(s) 127, 381, 412, 495, 887
PspPI GGNCC 2 cut(s) 410, 830
PspPPI RGGWCCY 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 2 cut(s) 622, 885
RsaI GTAC 3 cut(s) 217, 589, 947
RsaNI GTAC 3 cut(s) 216, 588, 946
RseI CAYNNNNRTG 2 cut(s) 672, 701
SapI GCTCTTC 1 cut(s) 607
SaqAI TTAA 7 cut(s) 18, 141, 255, 537, 627, 732, 976
SatI GCNGC 5 cut(s) 72, 270, 477, 848, 936
Sau3AI GATC 6 cut(s) 320, 445, 622, 707, 852, 885
Sau96I GGNCC 2 cut(s) 410, 830
ScaI AGTACT 1 cut(s) 589
ScrFI CCNGG 4 cut(s) 459, 523, 609, 930
SduI GDGCHC 1 cut(s) 130
SetI ASST 9 cut(s) 33, 94, 113, 385, 502, 567, 619, 725, 958
SfaNI GCATC 1 cut(s) 58
SinI GGWCC 2 cut(s) 410, 830
SmiMI CAYNNNNRTG 2 cut(s) 672, 701
SmlI CTYRAG 2 cut(s) 437, 751
SmoI CTYRAG 2 cut(s) 437, 751
SsiI CCGC 2 cut(s) 453, 833
SspMI CTAG 4 cut(s) 200, 405, 647, 687
StyD4I CCNGG 4 cut(s) 457, 521, 607, 928
TaaI ACNGT 3 cut(s) 277, 694, 817
TaqI TCGA 2 cut(s) 27, 531
TaqII GACCGA 1 cut(s) 893
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 2 cut(s) 230, 331
Tru1I TTAA 7 cut(s) 18, 141, 255, 537, 627, 732, 976
Tru9I TTAA 7 cut(s) 18, 141, 255, 537, 627, 732, 976
TscAI CASTG 2 cut(s) 495, 577
TseFI GTSAC 2 cut(s) 575, 694
TseI GCWGC 5 cut(s) 71, 269, 476, 847, 935
Tsp45I GTSAC 2 cut(s) 575, 694
TspDTI ATGAA 2 cut(s) 222, 323
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 2 cut(s) 495, 577
VpaK11BI GGWCC 2 cut(s) 410, 830
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 2 cut(s) 133, 533
XbaI TCTAGA 1 cut(s) 404
XceI RCATGY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 4 cut(s) 200, 405, 647, 687
ZrmI AGTACT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.