Rh7AG180600

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012611
Physical Location & Seq
Forward (+)
16065559 .. 16067066
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG180600.1

Sequence Viewer

Length: 1233 bp
ATGCAGGGACTTTCAATTAATAATTTCGACCTGAATGGAAATTCATATCCCCTAATATGGGGAGGAGATGCTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGCAAGCTTTTGTCCAAATGGAGCCCTGAATTCACACAAAGTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGATGATGGTTCGGGTGTTCTACTAGCCAATGGTGTCGGTACAATTATGGCCGATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTACAATAGGAGATGGACAGAGAATTATGAACTATATTAGATCAACAGCGAATCCAAATGCAACAATTCTTGTTGGCGAGACGGACATGGATATCATGGCACCTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCTATAACCCCAGATATCCTCAAGCCTGATCTGACCGCCCCTGGTGTTGACATACTTGCTGCTTGGTCTCCAGTGGCACCGCCTTCCATTTACAAAGATGACACCAGGAGTGTCGAATTTAATGTTATCTCCGGGACATCCATGTCCTGCCCCCATGCTAGTGGTGCTGCAGCTTATGTCAAGGCTGCACATCCAAATTGGTCTCCTGCTGCAATCAAATCTGCACTCATGACAACAGCTCATGTCCTGGATCCAAAGAAGCATGAAGACCTTGAATTCGCTTATGGGTCTGGTCACATCAACCCTCTGAAAGCAGTAGACCCCGGGCTTGTTTTTGATGCACATGTAGCAGACTATGTACATTTCCTCTGCAAGCAGGGTTACAATACCACCACTTTGAAGCTCATCACTGGTGACAACAGCAGTACTTGTGGCAGGACAAAGCCAGGAAGAGCTTGGGATCTTAACTATCCATCGTTCTCTCTAGCAGTTGAAGATGGCCAGCATATCAATGCTGTCTTTACTAGAACAGTCACAAATGTGGGATCACCAAACTCAACCTATACTATTAAGGCGCACACACCCTCCTCAAGTGTTTCGGTAAAACCATCAACCATTTCTTTCTCTTCTGTCGGTGAGAAGAAATCCTTCACAGTCAAGGTAATTGGTCCGCCTATTGCACAGCAGCCGATCATGTCCGGTGCAATCGTATTGACTGATGGGATCCATCTGGTGAGAAGCCCTCTCGTTATCTACACAATCCTCCCTGGTGCTGCACATTCGTACTCCATACCTGAGAAAAAAACCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

410

Amino Acids

43.2

Weight (kDa)

6.45

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 104 - 237 6.4e-21 Subtilase family
fn3_6 PF17766 293 - 390 7e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 559
AccB1I GGYRCC 2 cut(s) 379, 493
AccI GTMKAC 1 cut(s) 735
AciI CCGC 3 cut(s) 453, 497, 1088
AclWI GGATC 6 cut(s) 662, 675, 885, 970, 1135, 1148
AcoI YGGCCR 2 cut(s) 225, 916
AcsI RAATTY 4 cut(s) 40, 133, 533, 692
AfaI GTAC 4 cut(s) 217, 777, 844, 1202
AfiI CCNNNNNNNGG 2 cut(s) 57, 58
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 4 cut(s) 15, 692, 817, 911
AjnI CCWGG 5 cut(s) 457, 521, 663, 862, 1183
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 6 cut(s) 92, 111, 590, 656, 820, 872
AluI AGCT 6 cut(s) 92, 111, 590, 656, 820, 872
Alw26I GTCTC 3 cut(s) 353, 489, 624
AlwI GGATC 6 cut(s) 662, 675, 885, 970, 1135, 1148
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 2 cut(s) 225, 916
ApeKI GCWGC 9 cut(s) 71, 269, 476, 584, 587, 602, 626, 1102, 1190
ApoI RAATTY 4 cut(s) 40, 133, 533, 692
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AseI ATTAAT 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 2 cut(s) 410, 1085
AsuC2I CCSGG 3 cut(s) 550, 741, 742
AsuHPI GGTGA 4 cut(s) 842, 957, 1064, 1162
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 2 cut(s) 410, 1085
BalI TGGCCA 1 cut(s) 918
BamHI GGATCC 2 cut(s) 667, 1140
BanI GGYRCC 2 cut(s) 379, 493
BanII GRGCYC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 690
BbvI GCAGC 9 cut(s) 58, 281, 463, 571, 589, 599, 613, 1114, 1177
BccI CCATC 7 cut(s) 176, 287, 898, 908, 1033, 1130, 1152
BciT130I CCWGG 5 cut(s) 459, 523, 665, 864, 1185
BcnI CCSGG 3 cut(s) 550, 741, 742
BcoDI GTCTC 3 cut(s) 353, 489, 624
BfaI CTAG 5 cut(s) 200, 405, 576, 902, 942
BfmI CTRYAG 1 cut(s) 585
BisI GCNGC 9 cut(s) 72, 270, 477, 585, 588, 603, 627, 1103, 1191
BlsI GCNGC 9 cut(s) 73, 271, 478, 586, 589, 604, 628, 1104, 1192
BmcAI AGTACT 1 cut(s) 844
Bme1390I CCNGG 8 cut(s) 459, 523, 550, 665, 741, 742, 864, 1185
Bme18I GGWCC 2 cut(s) 410, 1085
BmeT110I CYCGRG 1 cut(s) 740
BmgT120I GGNCC 2 cut(s) 410, 1085
BmiI GGNNCC 6 cut(s) 127, 381, 412, 495, 669, 1142
BmrFI CCNGG 8 cut(s) 459, 523, 550, 665, 741, 742, 864, 1185
BmsI GCATC 2 cut(s) 58, 745
BpiI GAAGAC 1 cut(s) 690
BplI GAGNNNNNCTC 2 cut(s) 1144, 1176
BpmI CTGGAG 2 cut(s) 108, 471
BpuEI CTTGAG 2 cut(s) 422, 991
BpuMI CCSGG 3 cut(s) 550, 741, 742
BsaI GGTCTC 2 cut(s) 489, 624
BsaJI CCNNGG 4 cut(s) 457, 739, 740, 1183
BsaWI WCCGGW 1 cut(s) 1115
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 58
Bse1I ACTGG 2 cut(s) 488, 832
BseBI CCWGG 5 cut(s) 459, 523, 665, 864, 1185
BseDI CCNNGG 4 cut(s) 457, 739, 740, 1183
BseGI GGATG 2 cut(s) 554, 607
BseLI CCNNNNNNNGG 2 cut(s) 57, 58
BseMII CTCAG 1 cut(s) 1203
BseNI ACTGG 2 cut(s) 488, 832
BseRI GAGGAG 2 cut(s) 78, 994
BseXI GCAGC 9 cut(s) 58, 281, 463, 571, 589, 599, 613, 1114, 1177
BsgI GTGCAG 3 cut(s) 588, 624, 1176
BshFI GGCC 2 cut(s) 227, 918
BshNI GGYRCC 2 cut(s) 379, 493
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 3 cut(s) 549, 741, 1116
BslFI GGGAC 2 cut(s) 21, 565
BslI CCNNNNNNNGG 2 cut(s) 57, 58
BsmAI GTCTC 3 cut(s) 353, 489, 624
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 21, 565
BsnI GGCC 2 cut(s) 227, 918
Bso31I GGTCTC 2 cut(s) 489, 624
BsoBI CYCGRG 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
BspACI CCGC 3 cut(s) 453, 497, 1088
BspANI GGCC 2 cut(s) 227, 918
BspCNI CTCAG 1 cut(s) 1204
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 6 cut(s) 127, 381, 412, 495, 669, 1142
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 6 cut(s) 662, 675, 885, 970, 1135, 1148
BspQI GCTCTTC 1 cut(s) 862
BspT107I GGYRCC 2 cut(s) 379, 493
BspTNI GGTCTC 2 cut(s) 489, 624
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 2 cut(s) 488, 832
BssECI CCNNGG 4 cut(s) 457, 739, 740, 1183
BssMI GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
Bst2UI CCWGG 5 cut(s) 459, 523, 665, 864, 1185
Bst4CI ACNGT 3 cut(s) 277, 949, 1072
Bst6I CTCTTC 2 cut(s) 862, 1048
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 3 cut(s) 109, 791, 920
BstDEI CTNAG 1 cut(s) 1212
BstF5I GGATG 2 cut(s) 554, 607
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 7 cut(s) 323, 448, 670, 880, 965, 1110, 1143
BstMAI GTCTC 3 cut(s) 353, 489, 624
BstMBI GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
BstMWI GCNNNNNNNGC 2 cut(s) 581, 764
BstNI CCWGG 5 cut(s) 459, 523, 665, 864, 1185
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 8 cut(s) 457, 521, 548, 663, 739, 740, 862, 1183
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 9 cut(s) 58, 281, 463, 571, 589, 599, 613, 1114, 1177
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 3 cut(s) 667, 877, 1140
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 3 cut(s) 667, 877, 1140
BsuRI GGCC 2 cut(s) 227, 918
BtsCI GGATG 2 cut(s) 554, 607
BtsIMutI CAGTG 2 cut(s) 495, 825
Cac8I GCNNGC 3 cut(s) 109, 791, 920
CaiI CAGNNNCTG 1 cut(s) 275
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 2 cut(s) 410, 1085
Cfr9I CCCGGG 1 cut(s) 740
Csp6I GTAC 4 cut(s) 216, 776, 843, 1201
CviAII CATG 9 cut(s) 367, 376, 559, 572, 646, 659, 680, 761, 1111
CviQI GTAC 4 cut(s) 216, 776, 843, 1201
DdeI CTNAG 1 cut(s) 1212
DpnI GATC 7 cut(s) 322, 447, 669, 879, 964, 1109, 1142
DpnII GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 225, 916
Eam1104I CTCTTC 2 cut(s) 862, 1048
EarI CTCTTC 2 cut(s) 862, 1048
EciI GGCGGA 1 cut(s) 1077
Eco24I GRGCYC 1 cut(s) 130
Eco31I GGTCTC 2 cut(s) 489, 624
Eco32I GATATC 2 cut(s) 373, 433
Eco47I GGWCC 2 cut(s) 410, 1085
Eco88I CYCGRG 1 cut(s) 740
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 2 cut(s) 133, 692
EcoRII CCWGG 5 cut(s) 457, 521, 663, 862, 1183
EcoRV GATATC 2 cut(s) 373, 433
EcoT38I GRGCYC 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 9 cut(s) 370, 379, 562, 575, 649, 662, 683, 764, 1114
FalI AAGNNNNNCTT 2 cut(s) 1049, 1081
FaqI GGGAC 2 cut(s) 21, 565
FatI CATG 9 cut(s) 366, 375, 558, 571, 645, 658, 679, 760, 1110
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 9 cut(s) 72, 270, 477, 585, 588, 603, 627, 1103, 1191
FokI GGATG 2 cut(s) 541, 594
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 9 cut(s) 72, 270, 477, 585, 588, 603, 627, 1103, 1191
FspBI CTAG 5 cut(s) 200, 405, 576, 902, 942
GlaI GCGC 1 cut(s) 993
GluI GCNGC 9 cut(s) 72, 270, 477, 585, 588, 603, 627, 1103, 1191
GsuI CTGGAG 2 cut(s) 108, 471
HaeIII GGCC 2 cut(s) 227, 918
HapII CCGG 3 cut(s) 549, 741, 1116
HhaI GCGC 1 cut(s) 994
Hin1II CATG 9 cut(s) 370, 379, 562, 575, 649, 662, 683, 764, 1114
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 2 cut(s) 230, 331
HpaII CCGG 3 cut(s) 549, 741, 1116
HphI GGTGA 4 cut(s) 842, 957, 1064, 1162
Hpy166II GTNNAC 2 cut(s) 466, 736
Hpy188I TCNGA 4 cut(s) 100, 244, 450, 726
Hpy188III TCNNGA 2 cut(s) 405, 646
Hpy8I GTNNAC 2 cut(s) 466, 736
HpyAV CCTTC 2 cut(s) 510, 1075
HpyCH4III ACNGT 3 cut(s) 277, 949, 1072
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 764
HpyF3I CTNAG 1 cut(s) 1212
Hsp92II CATG 9 cut(s) 370, 379, 562, 575, 649, 662, 683, 764, 1114
HspAI GCGC 1 cut(s) 992
Kzo9I GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
LguI GCTCTTC 1 cut(s) 862
LmnI GCTCC 2 cut(s) 89, 125
Lsp1109I GCAGC 9 cut(s) 58, 281, 463, 571, 589, 599, 613, 1114, 1177
LweI GCATC 2 cut(s) 58, 745
MaeI CTAG 5 cut(s) 200, 405, 576, 902, 942
MaeIII GTNAC 5 cut(s) 280, 710, 797, 830, 949
MalI GATC 7 cut(s) 322, 447, 669, 879, 964, 1109, 1142
MboI GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
MboII GAAGA 7 cut(s) 228, 393, 695, 879, 923, 1035, 1069
MflI RGATCY 3 cut(s) 667, 877, 1140
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 918
MluNI TGGCCA 1 cut(s) 918
Mox20I TGGCCA 1 cut(s) 918
MroXI GAANNNNTTC 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 918
MseI TTAA 6 cut(s) 18, 255, 537, 882, 987, 1231
MslI CAYNNNNRTG 3 cut(s) 576, 927, 956
Msp20I TGGCCA 1 cut(s) 918
MspI CCGG 3 cut(s) 549, 741, 1116
MspR9I CCNGG 8 cut(s) 459, 523, 550, 665, 741, 742, 864, 1185
MvaI CCWGG 5 cut(s) 459, 523, 665, 864, 1185
MwoI GCNNNNNNNGC 2 cut(s) 581, 764
NciI CCSGG 3 cut(s) 550, 741, 742
NdeII GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
NlaIII CATG 9 cut(s) 370, 379, 562, 575, 649, 662, 683, 764, 1114
NlaIV GGNNCC 6 cut(s) 127, 381, 412, 495, 669, 1142
NmuCI GTSAC 3 cut(s) 710, 830, 949
NspI RCATGY 1 cut(s) 764
OliI CACNNNNGTG 1 cut(s) 956
PagI TCATGA 1 cut(s) 645
PciI ACATGT 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 1064
PfeI GAWTC 2 cut(s) 230, 331
PfoI TCCNGGA 2 cut(s) 548, 663
PkrI GCNGC 9 cut(s) 73, 271, 478, 586, 589, 604, 628, 1104, 1192
PpuMI RGGWCCY 1 cut(s) 410
PscI ACATGT 1 cut(s) 760
PshBI ATTAAT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 5 cut(s) 457, 521, 663, 862, 1183
PspGI CCWGG 5 cut(s) 457, 521, 663, 862, 1183
PspN4I GGNNCC 6 cut(s) 127, 381, 412, 495, 669, 1142
PspPI GGNCC 2 cut(s) 410, 1085
PspPPI RGGWCCY 1 cut(s) 410
PstI CTGCAG 1 cut(s) 589
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 3 cut(s) 667, 877, 1140
RsaI GTAC 4 cut(s) 217, 777, 844, 1202
RsaNI GTAC 4 cut(s) 216, 776, 843, 1201
RseI CAYNNNNRTG 3 cut(s) 576, 927, 956
SapI GCTCTTC 1 cut(s) 862
SaqAI TTAA 6 cut(s) 18, 255, 537, 882, 987, 1231
SatI GCNGC 9 cut(s) 72, 270, 477, 585, 588, 603, 627, 1103, 1191
Sau3AI GATC 7 cut(s) 320, 445, 667, 877, 962, 1107, 1140
Sau96I GGNCC 2 cut(s) 410, 1085
ScaI AGTACT 1 cut(s) 844
ScrFI CCNGG 8 cut(s) 459, 523, 550, 665, 741, 742, 864, 1185
SduI GDGCHC 1 cut(s) 130
SfaNI GCATC 2 cut(s) 58, 745
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 2 cut(s) 410, 1085
SmaI CCCGGG 1 cut(s) 742
SmiMI CAYNNNNRTG 3 cut(s) 576, 927, 956
SmlI CTYRAG 2 cut(s) 437, 1006
SmoI CTYRAG 2 cut(s) 437, 1006
SsiI CCGC 3 cut(s) 453, 497, 1088
SspMI CTAG 5 cut(s) 200, 405, 576, 902, 942
StyD4I CCNGG 8 cut(s) 457, 521, 548, 663, 739, 740, 862, 1183
TaaI ACNGT 3 cut(s) 277, 949, 1072
TaqI TCGA 2 cut(s) 27, 531
TatI WGTACW 2 cut(s) 775, 842
TfiI GAWTC 2 cut(s) 230, 331
Tru1I TTAA 6 cut(s) 18, 255, 537, 882, 987, 1231
Tru9I TTAA 6 cut(s) 18, 255, 537, 882, 987, 1231
TscAI CASTG 2 cut(s) 495, 832
TseFI GTSAC 3 cut(s) 710, 830, 949
TseI GCWGC 9 cut(s) 71, 269, 476, 584, 587, 602, 626, 1102, 1190
Tsp45I GTSAC 3 cut(s) 710, 830, 949
TspDTI ATGAA 4 cut(s) 33, 222, 323, 696
TspGWI ACGGA 1 cut(s) 377
TspMI CCCGGG 1 cut(s) 740
TspRI CASTG 2 cut(s) 495, 832
VpaK11BI GGWCC 2 cut(s) 410, 1085
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 4 cut(s) 40, 133, 533, 692
XbaI TCTAGA 1 cut(s) 404
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XmaI CCCGGG 1 cut(s) 740
XmiI GTMKAC 1 cut(s) 735
XmnI GAANNNNTTC 1 cut(s) 1064
XspI CTAG 5 cut(s) 200, 405, 576, 902, 942
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.