Rh7CG190100

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0012611
Physical Location & Seq
Forward (+)
16776786 .. 16778332
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG190100.1

Sequence Viewer

Length: 978 bp
ATGGCCAAGAGAGGAAGGTTCACATTGTATACATGGGAGAGCTGCACCATGGAGATTTTTCTGCTCAATCCACACACCATTCAATGCTACAAAGCCTTCAAGTTAGCCAAAGAGTCGCTGATCTATAGTTATGGGAAGAGCTTTAATGGGTTTGCCGCAAAGTTGTCGGATGAAGAAGTTGCAGAGTTGGCAGAAATAGAAGGTATAGTTTCAGTGATTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACGTGGAGCATCTGTAGGAGAAGAGGTTGTGATAGGGCTTCTAGACTCAGGAATCTGGCCGGAATCTGATAGCTTCAGTGATGATGGCTTTGGCCCTCCACCTAAGAAATGGAAAGGCAAATGCAAAGGTGCCAACTTCACCTGCAACAACAAAGTTATTGGAGCCCGCTACTACAACAGTGATGGCTCCTATGACCCCACTGACATCCAGTCCCCAAGAGATTCCAACGGACATGGAAGCCACACTGCTTCAACTGCAGCAGGCCGCGAGGTGCCAGCCAGTTACTTGGGGTTAGCTTCTGGAACTGCAAGAGGTGGAGTCCCAAATGCTAGGATTGCTGTCTACAAAGTTTGCTGGGCATCTGGATGTTATTCTTCAGATGTCCTTGCAGCATTCGATGATGCAATAGCAGATGGAGTTGATATTATATCGGTCTCACTTGGCAGTGACTTCCCACTCCAATACTTAGAAGACCCCATTGCTATTGGGTCTTTCCATGCCATGAAATATGGTATAGTGACCTCAACTTCTGCTGGAAACTCTGGCCCTGCTCCATTATCAGTTTCTAATTATGCACCATGGATAATGACCGTTGCTGCCAGCACCATTGACAGAAAATTTGTGGCAAAAGCAGTTCTTGGCAATGGAGAAGTCTATAGTGTGAGTTTGAAGCCTACATCAGCAGTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

325

Amino Acids

34.78

Weight (kDa)

6.31

Isoelectric Point (pI)

21.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 81 3.8e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 101 - 300 1.1e-22 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 2 cut(s) 413, 556
AccI GTMKAC 2 cut(s) 29, 627
AccII CGCG 1 cut(s) 552
AciI CCGC 3 cut(s) 156, 451, 550
AcoI YGGCCR 2 cut(s) 3, 341
AcsI RAATTY 1 cut(s) 902
AcuI CTGAAG 2 cut(s) 343, 645
AfaI GTAC 1 cut(s) 972
AgsI TTSAA 4 cut(s) 83, 100, 537, 955
AhdI GACNNNNNGTC 1 cut(s) 493
AluBI AGCT 5 cut(s) 42, 141, 238, 357, 581
AluI AGCT 5 cut(s) 42, 141, 238, 357, 581
Alw26I GTCTC 1 cut(s) 724
AoxI GGCC 6 cut(s) 3, 280, 341, 376, 547, 829
ApeKI GCWGC 4 cut(s) 42, 542, 674, 881
ApoI RAATTY 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
AspS9I GGNCC 2 cut(s) 377, 830
AsuHPI GGTGA 1 cut(s) 415
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 413, 556
BanII GRGCYC 1 cut(s) 451
BbsI GAAGAC 1 cut(s) 762
BbvI GCAGC 4 cut(s) 29, 554, 686, 868
BccI CCATC 3 cut(s) 362, 461, 692
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BcoDI GTCTC 1 cut(s) 724
BfaI CTAG 2 cut(s) 326, 615
BfmI CTRYAG 4 cut(s) 124, 297, 540, 940
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 6 cut(s) 43, 156, 543, 550, 675, 882
BlsI GCNGC 6 cut(s) 44, 157, 544, 551, 676, 883
BmeRI GACNNNNNGTC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 377, 830
BmiI GGNNCC 4 cut(s) 415, 448, 472, 558
BmsI GCATC 3 cut(s) 302, 653, 676
BpiI GAAGAC 1 cut(s) 762
BsaI GGTCTC 1 cut(s) 724
BsaJI CCNNGG 2 cut(s) 48, 863
BsaXI ACNNNNNCTCC 4 cut(s) 282, 294, 312, 324
Bse1I ACTGG 2 cut(s) 493, 564
Bse3DI GCAATG 2 cut(s) 762, 934
BseDI CCNNGG 2 cut(s) 48, 863
BseGI GGATG 3 cut(s) 175, 489, 656
BseMI GCAATG 2 cut(s) 762, 934
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 493, 564
BseXI GCAGC 4 cut(s) 29, 554, 686, 868
BseYI CCCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 6 cut(s) 5, 282, 343, 378, 549, 831
BshNI GGYRCC 2 cut(s) 413, 556
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 3 cut(s) 272, 481, 590
BsmAI GTCTC 1 cut(s) 724
BsmFI GGGAC 3 cut(s) 272, 481, 590
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 6 cut(s) 5, 282, 343, 378, 549, 831
Bso31I GGTCTC 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 1 cut(s) 120
Bsp19I CCATGG 2 cut(s) 48, 863
BspACI CCGC 3 cut(s) 156, 451, 550
BspANI GGCC 6 cut(s) 5, 282, 343, 378, 549, 831
BspCNI CTCAG 1 cut(s) 344
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 4 cut(s) 415, 448, 472, 558
BspMAI CTGCAG 1 cut(s) 544
BspMI ACCTGC 1 cut(s) 434
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 2 cut(s) 413, 556
BspTNI GGTCTC 1 cut(s) 724
BsrDI GCAATG 2 cut(s) 762, 934
BsrI ACTGG 2 cut(s) 493, 564
BssECI CCNNGG 2 cut(s) 48, 863
BssMI GATC 1 cut(s) 120
BssNAI GTATAC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 48, 863
Bst1107I GTATAC 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 464, 877
Bst6I CTCTTC 2 cut(s) 131, 300
BstC8I GCNNGC 4 cut(s) 451, 547, 561, 886
BstDEI CTNAG 3 cut(s) 331, 387, 751
BstDSI CCRYGG 2 cut(s) 48, 863
BstF5I GGATG 3 cut(s) 175, 489, 656
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 1 cut(s) 123
BstMAI GTCTC 1 cut(s) 724
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 3 cut(s) 188, 539, 620
BstSFI CTRYAG 4 cut(s) 124, 297, 540, 940
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 4 cut(s) 29, 554, 686, 868
BstV2I GAAGAC 1 cut(s) 762
BstXI CCANNNNNNTGG 1 cut(s) 571
BstZ17I GTATAC 1 cut(s) 30
BsuRI GGCC 6 cut(s) 5, 282, 343, 378, 549, 831
BtgI CCRYGG 2 cut(s) 48, 863
BtsCI GGATG 3 cut(s) 175, 489, 656
BtsI GCAGTG 2 cut(s) 528, 736
BtsIMutI CAGTG 6 cut(s) 219, 367, 469, 483, 528, 736
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 451, 547, 561, 886
Cfr13I GGNCC 2 cut(s) 377, 830
Csp6I GTAC 1 cut(s) 971
CviAII CATG 7 cut(s) 33, 49, 265, 518, 782, 787, 864
CviQI GTAC 1 cut(s) 971
DdeI CTNAG 3 cut(s) 331, 387, 751
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
DriI GACNNNNNGTC 1 cut(s) 493
EaeI YGGCCR 2 cut(s) 3, 341
Eam1104I CTCTTC 2 cut(s) 131, 300
Eam1105I GACNNNNNGTC 1 cut(s) 493
EarI CTCTTC 2 cut(s) 131, 300
Eco130I CCWWGG 2 cut(s) 48, 863
Eco24I GRGCYC 1 cut(s) 451
Eco31I GGTCTC 1 cut(s) 724
Eco57I CTGAAG 2 cut(s) 343, 645
EcoT14I CCWWGG 2 cut(s) 48, 863
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 2 cut(s) 48, 863
FaeI CATG 7 cut(s) 36, 52, 268, 521, 785, 790, 867
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 3 cut(s) 272, 481, 590
FatI CATG 7 cut(s) 32, 48, 264, 517, 781, 786, 863
FauI CCCGC 1 cut(s) 458
FblI GTMKAC 2 cut(s) 29, 627
Fnu4HI GCNGC 6 cut(s) 43, 156, 543, 550, 675, 882
FokI GGATG 3 cut(s) 182, 476, 663
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 6 cut(s) 43, 156, 543, 550, 675, 882
FspBI CTAG 2 cut(s) 326, 615
GluI GCNGC 6 cut(s) 43, 156, 543, 550, 675, 882
GsaI CCCAGC 1 cut(s) 643
HaeIII GGCC 6 cut(s) 5, 282, 343, 378, 549, 831
HapII CCGG 1 cut(s) 344
Hin1II CATG 7 cut(s) 36, 52, 268, 521, 785, 790, 867
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 7 cut(s) 113, 217, 329, 336, 347, 506, 603
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 3 cut(s) 21, 30, 628
Hpy188I TCNGA 3 cut(s) 169, 352, 664
Hpy188III TCNNGA 4 cut(s) 326, 333, 585, 648
Hpy8I GTNNAC 3 cut(s) 21, 30, 628
HpyAV CCTTC 3 cut(s) 9, 106, 194
HpyCH4III ACNGT 2 cut(s) 464, 877
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 9 cut(s) 45, 182, 408, 429, 542, 593, 674, 689, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 539, 620
HpyF3I CTNAG 3 cut(s) 331, 387, 751
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 7 cut(s) 36, 52, 268, 521, 785, 790, 867
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 4 cut(s) 290, 446, 476, 841
Lsp1109I GCAGC 4 cut(s) 29, 554, 686, 868
LweI GCATC 3 cut(s) 302, 653, 676
MaeI CTAG 2 cut(s) 326, 615
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 3 cut(s) 566, 731, 802
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 5 cut(s) 148, 185, 317, 651, 767
MhlI GDGCHC 1 cut(s) 451
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 853, 902
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 122, 323, 612
MmeI TCCRAC 2 cut(s) 147, 534
MnlI CCTC 6 cut(s) 5, 301, 390, 547, 590, 817
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 649
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 344
Mva1269I GAATGC 1 cut(s) 677
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 3 cut(s) 188, 539, 620
NcoI CCATGG 2 cut(s) 48, 863
NdeII GATC 1 cut(s) 120
NlaIII CATG 7 cut(s) 36, 52, 268, 521, 785, 790, 867
NlaIV GGNNCC 4 cut(s) 415, 448, 472, 558
NmuCI GTSAC 2 cut(s) 731, 802
PaqCI CACCTGC 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 4 cut(s) 217, 336, 347, 506
PkrI GCNGC 6 cut(s) 44, 157, 544, 551, 676, 883
PleI GAGTC 3 cut(s) 121, 323, 611
PpsI GAGTC 3 cut(s) 121, 323, 611
PspFI CCCAGC 1 cut(s) 639
PspN4I GGNNCC 4 cut(s) 415, 448, 472, 558
PspPI GGNCC 2 cut(s) 377, 830
PstI CTGCAG 1 cut(s) 544
RsaI GTAC 1 cut(s) 972
RsaNI GTAC 1 cut(s) 971
RseI CAYNNNNRTG 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 6 cut(s) 43, 156, 543, 550, 675, 882
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 2 cut(s) 377, 830
SchI GAGTC 3 cut(s) 122, 323, 612
SduI GDGCHC 1 cut(s) 451
SfaNI GCATC 3 cut(s) 302, 653, 676
SfcI CTRYAG 4 cut(s) 124, 297, 540, 940
SmiMI CAYNNNNRTG 1 cut(s) 649
Sse9I AATT 2 cut(s) 853, 902
SsiI CCGC 3 cut(s) 156, 451, 550
SspMI CTAG 2 cut(s) 326, 615
StyI CCWWGG 2 cut(s) 48, 863
TaaI ACNGT 2 cut(s) 464, 877
TaiI ACGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 681
TaqII GACCGA 1 cut(s) 706
TasI AATT 2 cut(s) 853, 902
TatI WGTACW 1 cut(s) 970
TauI GCSGC 2 cut(s) 158, 552
TfiI GAWTC 4 cut(s) 217, 336, 347, 506
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 6 cut(s) 219, 367, 469, 490, 535, 736
TseFI GTSAC 2 cut(s) 731, 802
TseI GCWGC 4 cut(s) 42, 542, 674, 881
Tsp45I GTSAC 2 cut(s) 731, 802
TspDTI ATGAA 3 cut(s) 186, 253, 803
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 6 cut(s) 219, 367, 469, 490, 535, 736
XapI RAATTY 1 cut(s) 902
XbaI TCTAGA 1 cut(s) 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 2 cut(s) 29, 627
XspI CTAG 2 cut(s) 326, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.