RLG00000003781

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012611
Physical Location & Seq
Reverse (-)
52637986 .. 52641158
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003781

Sequence Viewer

Length: 2148 bp
ATGGCCAAGAGAGGAAGCTTCAAGTCGGCCAAAGAGTCGCTGATCTATAGCTATGGGAAGAGCTTTAATGGGTTTGCCGCAAAGTTGTTGGATGAAGAAGTTGCAGAGTTGGCAGACATGGAAGGTATAGTTTCCGTGGTTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACTTGGAGCATCTGTAGGAGGAGAGGTTGTGATAGGGCTTCTAGACTCAGGAATCTGGCCGGAATCTGATAGCTTCAGTGATGATGGCTTTGGCCCTCCACCTAAGAAATGGAAAGGCAAATGCAAAGGTGCCAACTTCACCTGCAACAACAAAGTTATTGGAGCCCGCTACTACAACAGTGATGGCACCTATGACCCCACTGACATCCAGTCCCCAAGAGATTCCAACGGACATGGAAGCCACACTGCTTCAACTGCAGCAGGCCGCGAGGTGACAGCCAGTTACTTGGGGTCGTTAGCTTCTGGATATGGAACCGCAAGAGGTGGAGTCCAGAATGCTAGGATTGCTGTCTACAAAGTTTGCTGGGCATCTGGATGTTATTCTTCAGATGTCCTTGCAGCATTTGATGATGCAATAGCAGATGGAGTCGATATTATATCGGTCTCACTTGGCAGTGACTCCCCACTCCAATACTTAGAAGACCCCATTGCTATTGGGTCTTTCCATGCCATGAAATATGGTATTGTGACCTCAACTTCCGCTGGAAACTCTGGCCCTGCTCAATTATCAGTTTCTAATTATGCACCATGGATAATGACCGTTGCTGCCAGCACCATTGACAGAAAATTTGTGGCAAAAGCAGTTCTTGGCAATGGAGAAGTCTATAGTGGACTTTCAATTAATAATTTCGGCCTGAATGGAAAGTCATATCCCCTAATATGGGGAGGAGATGCTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGCAAGCTTTTGTCCAAATGGAGCCCTGAATTCACAGGGAGTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGACGATGGTTCGGGTGTTCTACTAGCCAGTGGTGTTGGTACAATTATGGCTGATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTACAATAGGAGATGGACAGAGAATTATGAACTATATCAGATCAACAGCGAATCCAAATGCAACAATTCTTGTTGGCGAGACGGACATGGATATCATGGCACCTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCTATAACCCCAGATATCCTCAAGCCTGATCTGACCGCCCCAGGTGTTGACATACTTGCTGCTTGGTCTCCAGTGGCACCGCCTTCCATTTACAAAGATGACACCAGGAGTGTCGAAATTAATGTTATCTCCGGGACATCCATGTCCTGCCCTCATGCTAGTGGTGCTGCAGCTTATGTCAAGGCTGCACATCCAAATTGGTCTCCTGCTGCAATCATATCTGCACTCATGACAACAGCTCATGTCCTGGACCCAAAGAAGCATGAAGACCTCGAATTCGCTTATGGGTCTGGTCACATCAACCCTCTGAAAGCAGTAGACCCCGGGCTTGTTTTTGATGCACATGTAGCAGACTATGTACATTTCCTTTGCAAGCAGGGTTACAATACCACCACTTTGAAGCTCATCACTGGTGACAACAGCAGTACTTGTGGCAGGACAAAGCCAGGAAGAGCTTGGGATCTTAACTATCCATCGTTCTCCCTAGCAGTTGAAGATGGCCGGCATATCAATGCTGTCTTTACTAGAACAGTCACAAATGTGGGATCACCAAACTCAACCTATACTATTAAGGCGCACACACCCTCCTCAAGTGTTTCGGTAAAACCATCAACCATTTCTTTCTCTTCTGTCGGTGAGAAGAAATCCTTCACAGTCAAGGTAATTGGTCCGCCTATTGCACAGCAGCCCATCATGTCCGGTGCAATCGTGTTGACTGATGGGATCCATCTGGTGAGAAGCCCTCTCGTTATCTACACAATCCTCCCCGGTGCTGCACATTCGTACTCCATACCTGAGAAAAAACCAAATTTTAAAGGTTCTTCTTTGTACCACAGAAATGGGATCTTCAGACACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

716

Amino Acids

75.27

Weight (kDa)

6.65

Isoelectric Point (pI)

24.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 6 - 55 1e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 76 - 527 3.2e-43 Subtilase family
fn3_6 PF17766 583 - 680 2.3e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 356
AasI GACNNNNNNGTC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 356
AccB1I GGYRCC 4 cut(s) 335, 392, 1249, 1363
AccI GTMKAC 2 cut(s) 558, 1605
AccII CGCG 1 cut(s) 474
AciI CCGC 8 cut(s) 78, 373, 472, 522, 747, 1323, 1367, 1958
AclWI GGATC 5 cut(s) 1755, 1840, 2005, 2018, 2138
AcoI YGGCCR 4 cut(s) 3, 27, 263, 1786
AcsI RAATTY 4 cut(s) 833, 1003, 1562, 2095
AcuI CTGAAG 3 cut(s) 265, 576, 2119
AfaI GTAC 5 cut(s) 1087, 1647, 1714, 2072, 2117
AfiI CCNNNNNNNGG 2 cut(s) 927, 928
AflIII ACRYGT 1 cut(s) 1630
AgsI TTSAA 5 cut(s) 22, 459, 885, 1687, 1781
AhdI GACNNNNNGTC 1 cut(s) 415
AjnI CCWGG 4 cut(s) 1327, 1391, 1533, 1732
AleI CACNNNNGTG 1 cut(s) 1826
Alw26I GTCTC 4 cut(s) 655, 1223, 1359, 1494
AlwI GGATC 5 cut(s) 1755, 1840, 2005, 2018, 2138
AlwNI CAGNNNCTG 1 cut(s) 1145
Ama87I CYCGRG 1 cut(s) 1610
AoxI GGCC 9 cut(s) 3, 27, 202, 263, 298, 469, 760, 898, 1786
ApoI RAATTY 4 cut(s) 833, 1003, 1562, 2095
ArsI GACNNNNNNTTYG 4 cut(s) 818, 850, 1547, 1579
AseI ATTAAT 2 cut(s) 888, 1407
Asp700I GAANNNNTTC 1 cut(s) 1934
AspLEI GCGC 1 cut(s) 1864
AspS9I GGNCC 5 cut(s) 299, 761, 1280, 1537, 1955
AsuC2I CCSGG 4 cut(s) 1420, 1611, 1612, 2055
AsuHPI GGTGA 6 cut(s) 337, 490, 1712, 1827, 1934, 2032
AvaI CYCGRG 1 cut(s) 1610
AvaII GGWCC 3 cut(s) 1280, 1537, 1955
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 2010
BanI GGYRCC 4 cut(s) 335, 392, 1249, 1363
BanII GRGCYC 2 cut(s) 373, 1000
BbsI GAAGAC 2 cut(s) 693, 1560
BciT130I CCWGG 4 cut(s) 1329, 1393, 1535, 1734
BcnI CCSGG 4 cut(s) 1420, 1611, 1612, 2055
BcoDI GTCTC 4 cut(s) 655, 1223, 1359, 1494
BfaI CTAG 7 cut(s) 248, 546, 1070, 1275, 1446, 1772, 1812
BfmI CTRYAG 5 cut(s) 46, 219, 462, 871, 1455
BfuAI ACCTGC 1 cut(s) 356
BmcAI AGTACT 1 cut(s) 1714
Bme1390I CCNGG 8 cut(s) 1329, 1393, 1420, 1535, 1611, 1612, 1734, 2055
Bme18I GGWCC 3 cut(s) 1280, 1537, 1955
BmeRI GACNNNNNGTC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 1610
BmgT120I GGNCC 5 cut(s) 299, 761, 1280, 1537, 1955
BmrFI CCNGG 8 cut(s) 1329, 1393, 1420, 1535, 1611, 1612, 1734, 2055
BmsI GCATC 5 cut(s) 224, 584, 607, 928, 1615
BpiI GAAGAC 2 cut(s) 693, 1560
BplI GAGNNNNNCTC 2 cut(s) 2014, 2046
BpmI CTGGAG 2 cut(s) 978, 1341
BpuEI CTTGAG 2 cut(s) 1292, 1861
BpuMI CCSGG 4 cut(s) 1420, 1611, 1612, 2055
BsaI GGTCTC 3 cut(s) 655, 1359, 1494
BsaJI CCNNGG 6 cut(s) 135, 794, 1327, 1609, 1610, 2053
BsaWI WCCGGW 1 cut(s) 1985
BsaXI ACNNNNNCTCC 8 cut(s) 204, 216, 219, 234, 246, 249, 1856, 1886
Bsc4I CCNNNNNNNGG 2 cut(s) 927, 928
Bse118I RCCGGY 1 cut(s) 1788
Bse1I ACTGG 5 cut(s) 415, 486, 1074, 1358, 1702
Bse3DI GCAATG 2 cut(s) 693, 865
BseBI CCWGG 4 cut(s) 1329, 1393, 1535, 1734
BseDI CCNNGG 6 cut(s) 135, 794, 1327, 1609, 1610, 2053
BseGI GGATG 5 cut(s) 97, 411, 587, 1424, 1477
BseLI CCNNNNNNNGG 2 cut(s) 927, 928
BseMI GCAATG 2 cut(s) 693, 865
BseMII CTCAG 2 cut(s) 267, 2073
BseNI ACTGG 5 cut(s) 415, 486, 1074, 1358, 1702
BseRI GAGGAG 3 cut(s) 240, 948, 1864
BseYI CCCAGC 1 cut(s) 570
BsgI GTGCAG 3 cut(s) 1458, 1494, 2046
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 9 cut(s) 5, 29, 204, 265, 300, 471, 762, 900, 1788
BshNI GGYRCC 4 cut(s) 335, 392, 1249, 1363
BsiHKCI CYCGRG 1 cut(s) 1610
BsiSI CCGG 6 cut(s) 266, 1419, 1611, 1789, 1986, 2055
BslFI GGGAC 3 cut(s) 194, 403, 1435
BslI CCNNNNNNNGG 2 cut(s) 927, 928
BsmAI GTCTC 4 cut(s) 655, 1223, 1359, 1494
BsmBI CGTCTC 1 cut(s) 1223
BsmFI GGGAC 3 cut(s) 194, 403, 1435
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 9 cut(s) 5, 29, 204, 265, 300, 471, 762, 900, 1788
Bso31I GGTCTC 3 cut(s) 655, 1359, 1494
BsoBI CYCGRG 1 cut(s) 1610
Bsp1286I GDGCHC 2 cut(s) 373, 1000
Bsp1407I TGTACA 1 cut(s) 1645
Bsp143I GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
Bsp19I CCATGG 1 cut(s) 794
BspACI CCGC 8 cut(s) 78, 373, 472, 522, 747, 1323, 1367, 1958
BspANI GGCC 9 cut(s) 5, 29, 204, 265, 300, 471, 762, 900, 1788
BspCNI CTCAG 2 cut(s) 266, 2074
BspFNI CGCG 1 cut(s) 474
BspHI TCATGA 1 cut(s) 1515
BspMAI CTGCAG 2 cut(s) 466, 1459
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 5 cut(s) 1755, 1840, 2005, 2018, 2138
BspQI GCTCTTC 2 cut(s) 53, 1732
BspT107I GGYRCC 4 cut(s) 335, 392, 1249, 1363
BspTNI GGTCTC 3 cut(s) 655, 1359, 1494
BsrDI GCAATG 2 cut(s) 693, 865
BsrFI RCCGGY 1 cut(s) 1788
BsrGI TGTACA 1 cut(s) 1645
BsrI ACTGG 5 cut(s) 415, 486, 1074, 1358, 1702
BssAI RCCGGY 1 cut(s) 1788
BssECI CCNNGG 6 cut(s) 135, 794, 1327, 1609, 1610, 2053
BssMI GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
BssT1I CCWWGG 1 cut(s) 794
Bst2UI CCWGG 4 cut(s) 1329, 1393, 1535, 1734
Bst4CI ACNGT 5 cut(s) 386, 808, 1147, 1819, 1942
Bst6I CTCTTC 3 cut(s) 53, 1732, 1918
BstAUI TGTACA 1 cut(s) 1645
BstC8I GCNNGC 6 cut(s) 373, 469, 817, 979, 1661, 1790
BstDEI CTNAG 4 cut(s) 253, 309, 682, 2082
BstDSI CCRYGG 2 cut(s) 135, 794
BstF5I GGATG 5 cut(s) 97, 411, 587, 1424, 1477
BstFNI CGCG 1 cut(s) 474
BstHHI GCGC 1 cut(s) 1864
BstKTI GATC 7 cut(s) 45, 1193, 1318, 1750, 1835, 2013, 2133
BstMAI GTCTC 4 cut(s) 655, 1223, 1359, 1494
BstMBI GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
BstMWI GCNNNNNNNGC 5 cut(s) 110, 461, 551, 1451, 1634
BstNI CCWGG 4 cut(s) 1329, 1393, 1535, 1734
BstNSI RCATGY 1 cut(s) 1634
BstSCI CCNGG 8 cut(s) 1327, 1391, 1418, 1533, 1609, 1610, 1732, 2053
BstSFI CTRYAG 5 cut(s) 46, 219, 462, 871, 1455
BstUI CGCG 1 cut(s) 474
BstV2I GAAGAC 2 cut(s) 693, 1560
BstX2I RGATCY 3 cut(s) 1747, 2010, 2130
BstXI CCANNNNNNTGG 2 cut(s) 493, 2126
BstYI RGATCY 3 cut(s) 1747, 2010, 2130
BsuRI GGCC 9 cut(s) 5, 29, 204, 265, 300, 471, 762, 900, 1788
BtgI CCRYGG 2 cut(s) 135, 794
BtsCI GGATG 5 cut(s) 97, 411, 587, 1424, 1477
BtsI GCAGTG 2 cut(s) 450, 667
BtsIMutI CAGTG 8 cut(s) 289, 391, 405, 450, 667, 1081, 1365, 1695
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 6 cut(s) 373, 469, 817, 979, 1661, 1790
CaiI CAGNNNCTG 1 cut(s) 1145
CciI TCATGA 1 cut(s) 1515
CfoI GCGC 1 cut(s) 1864
Cfr10I RCCGGY 1 cut(s) 1788
Cfr13I GGNCC 5 cut(s) 299, 761, 1280, 1537, 1955
Cfr9I CCCGGG 1 cut(s) 1610
Csp6I GTAC 5 cut(s) 1086, 1646, 1713, 2071, 2116
CviQI GTAC 5 cut(s) 1086, 1646, 1713, 2071, 2116
DdeI CTNAG 4 cut(s) 253, 309, 682, 2082
DpnI GATC 7 cut(s) 44, 1192, 1317, 1749, 1834, 2012, 2132
DpnII GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
DraI TTTAAA 1 cut(s) 2101
DrdI GACNNNNNNGTC 1 cut(s) 1429
DriI GACNNNNNGTC 1 cut(s) 415
DseDI GACNNNNNNGTC 1 cut(s) 1429
EaeI YGGCCR 4 cut(s) 3, 27, 263, 1786
Eam1104I CTCTTC 3 cut(s) 53, 1732, 1918
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 3 cut(s) 53, 1732, 1918
EciI GGCGGA 1 cut(s) 1947
Eco130I CCWWGG 1 cut(s) 794
Eco24I GRGCYC 2 cut(s) 373, 1000
Eco31I GGTCTC 3 cut(s) 655, 1359, 1494
Eco32I GATATC 2 cut(s) 1243, 1303
Eco47I GGWCC 3 cut(s) 1280, 1537, 1955
Eco57I CTGAAG 3 cut(s) 265, 576, 2119
Eco88I CYCGRG 1 cut(s) 1610
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRI GAATTC 2 cut(s) 1003, 1562
EcoRII CCWGG 4 cut(s) 1327, 1391, 1533, 1732
EcoRV GATATC 2 cut(s) 1243, 1303
EcoT14I CCWWGG 1 cut(s) 794
EcoT38I GRGCYC 2 cut(s) 373, 1000
ErhI CCWWGG 1 cut(s) 794
Esp3I CGTCTC 1 cut(s) 1223
FalI AAGNNNNNCTT 6 cut(s) 192, 224, 837, 869, 1919, 1951
FaqI GGGAC 3 cut(s) 194, 403, 1435
FauI CCCGC 1 cut(s) 380
FblI GTMKAC 2 cut(s) 558, 1605
FokI GGATG 5 cut(s) 104, 398, 594, 1411, 1464
FriOI GRGCYC 2 cut(s) 373, 1000
FspBI CTAG 7 cut(s) 248, 546, 1070, 1275, 1446, 1772, 1812
GlaI GCGC 1 cut(s) 1863
GsaI CCCAGC 1 cut(s) 574
GsuI CTGGAG 2 cut(s) 978, 1341
HaeIII GGCC 9 cut(s) 5, 29, 204, 265, 300, 471, 762, 900, 1788
HapII CCGG 6 cut(s) 266, 1419, 1611, 1789, 1986, 2055
HhaI GCGC 1 cut(s) 1864
Hin6I GCGC 1 cut(s) 1862
HinP1I GCGC 1 cut(s) 1862
HincII GTYRAC 2 cut(s) 1336, 2001
HindII GTYRAC 2 cut(s) 1336, 2001
HindIII AAGCTT 3 cut(s) 16, 158, 979
HpaII CCGG 6 cut(s) 266, 1419, 1611, 1789, 1986, 2055
HphI GGTGA 6 cut(s) 337, 490, 1712, 1827, 1934, 2032
Hpy166II GTNNAC 5 cut(s) 559, 878, 1336, 1606, 2001
Hpy188I TCNGA 8 cut(s) 274, 595, 970, 1114, 1190, 1320, 1596, 2138
Hpy188III TCNNGA 7 cut(s) 248, 255, 510, 538, 579, 1275, 1516
Hpy8I GTNNAC 5 cut(s) 559, 878, 1336, 1606, 2001
HpyAV CCTTC 3 cut(s) 116, 1380, 1945
HpyCH4III ACNGT 5 cut(s) 386, 808, 1147, 1819, 1942
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 461, 551, 1451, 1634
HpyF3I CTNAG 4 cut(s) 253, 309, 682, 2082
HspAI GCGC 1 cut(s) 1862
KroI GCCGGC 1 cut(s) 1788
KroNI GCCGGC 1 cut(s) 1790
Kzo9I GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
LguI GCTCTTC 2 cut(s) 53, 1732
LmnI GCTCC 4 cut(s) 212, 368, 959, 995
LweI GCATC 5 cut(s) 224, 584, 607, 928, 1615
MaeI CTAG 7 cut(s) 248, 546, 1070, 1275, 1446, 1772, 1812
MaeIII GTNAC 9 cut(s) 478, 488, 662, 733, 1150, 1580, 1667, 1700, 1819
MalI GATC 7 cut(s) 44, 1192, 1317, 1749, 1834, 2012, 2132
MboI GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
MflI RGATCY 3 cut(s) 1747, 2010, 2130
MhlI GDGCHC 2 cut(s) 373, 1000
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 44, 245, 543, 642, 659
MmeI TCCRAC 2 cut(s) 69, 456
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 1788
MroXI GAANNNNTTC 1 cut(s) 1934
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 66, 888, 1125, 1407, 1752, 1857, 2100
MslI CAYNNNNRTG 5 cut(s) 580, 1446, 1797, 1826, 2124
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 6 cut(s) 266, 1419, 1611, 1789, 1986, 2055
MspR9I CCNGG 8 cut(s) 1329, 1393, 1420, 1535, 1611, 1612, 1734, 2055
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 4 cut(s) 1329, 1393, 1535, 1734
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 5 cut(s) 110, 461, 551, 1451, 1634
NaeI GCCGGC 1 cut(s) 1790
NciI CCSGG 4 cut(s) 1420, 1611, 1612, 2055
NcoI CCATGG 1 cut(s) 794
NdeII GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
NgoMIV GCCGGC 1 cut(s) 1788
NmuCI GTSAC 6 cut(s) 478, 662, 733, 1580, 1700, 1819
NspI RCATGY 1 cut(s) 1634
OliI CACNNNNGTG 1 cut(s) 1826
PagI TCATGA 1 cut(s) 1515
PaqCI CACCTGC 1 cut(s) 356
PciI ACATGT 1 cut(s) 1630
PciSI GCTCTTC 2 cut(s) 53, 1732
PctI GAATGC 1 cut(s) 547
PdiI GCCGGC 1 cut(s) 1790
PdmI GAANNNNTTC 1 cut(s) 1934
PfeI GAWTC 5 cut(s) 258, 269, 428, 1100, 1201
PfoI TCCNGGA 2 cut(s) 1418, 1533
PleI GAGTC 5 cut(s) 43, 245, 542, 641, 659
PpsI GAGTC 5 cut(s) 43, 245, 542, 641, 659
PpuMI RGGWCCY 1 cut(s) 1280
PscI ACATGT 1 cut(s) 1630
PshBI ATTAAT 2 cut(s) 888, 1407
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 4 cut(s) 1327, 1391, 1533, 1732
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 4 cut(s) 1327, 1391, 1533, 1732
PspPI GGNCC 5 cut(s) 299, 761, 1280, 1537, 1955
PspPPI RGGWCCY 1 cut(s) 1280
PstI CTGCAG 2 cut(s) 466, 1459
PstNI CAGNNNCTG 1 cut(s) 1145
PsuI RGATCY 3 cut(s) 1747, 2010, 2130
RsaI GTAC 5 cut(s) 1087, 1647, 1714, 2072, 2117
RsaNI GTAC 5 cut(s) 1086, 1646, 1713, 2071, 2116
RseI CAYNNNNRTG 5 cut(s) 580, 1446, 1797, 1826, 2124
SapI GCTCTTC 2 cut(s) 53, 1732
SaqAI TTAA 7 cut(s) 66, 888, 1125, 1407, 1752, 1857, 2100
Sau3AI GATC 7 cut(s) 42, 1190, 1315, 1747, 1832, 2010, 2130
Sau96I GGNCC 5 cut(s) 299, 761, 1280, 1537, 1955
ScaI AGTACT 1 cut(s) 1714
SchI GAGTC 5 cut(s) 44, 245, 543, 642, 659
ScrFI CCNGG 8 cut(s) 1329, 1393, 1420, 1535, 1611, 1612, 1734, 2055
SduI GDGCHC 2 cut(s) 373, 1000
SfaNI GCATC 5 cut(s) 224, 584, 607, 928, 1615
SfcI CTRYAG 5 cut(s) 46, 219, 462, 871, 1455
SinI GGWCC 3 cut(s) 1280, 1537, 1955
SmaI CCCGGG 1 cut(s) 1612
SmiMI CAYNNNNRTG 5 cut(s) 580, 1446, 1797, 1826, 2124
SmlI CTYRAG 2 cut(s) 1307, 1876
SmoI CTYRAG 2 cut(s) 1307, 1876
SsiI CCGC 8 cut(s) 78, 373, 472, 522, 747, 1323, 1367, 1958
SspMI CTAG 7 cut(s) 248, 546, 1070, 1275, 1446, 1772, 1812
StyD4I CCNGG 8 cut(s) 1327, 1391, 1418, 1533, 1609, 1610, 1732, 2053
StyI CCWWGG 1 cut(s) 794
TaaI ACNGT 5 cut(s) 386, 808, 1147, 1819, 1942
TaqI TCGA 3 cut(s) 636, 1401, 1560
TaqII GACCGA 1 cut(s) 637
TatI WGTACW 2 cut(s) 1645, 1712
TauI GCSGC 2 cut(s) 80, 474
TfiI GAWTC 5 cut(s) 258, 269, 428, 1100, 1201
Tru1I TTAA 7 cut(s) 66, 888, 1125, 1407, 1752, 1857, 2100
Tru9I TTAA 7 cut(s) 66, 888, 1125, 1407, 1752, 1857, 2100
TscAI CASTG 8 cut(s) 289, 391, 412, 457, 667, 1081, 1365, 1702
TseFI GTSAC 6 cut(s) 478, 662, 733, 1580, 1700, 1819
Tsp45I GTSAC 6 cut(s) 478, 662, 733, 1580, 1700, 1819
TspDTI ATGAA 6 cut(s) 108, 175, 734, 1092, 1193, 1566
TspGWI ACGGA 3 cut(s) 124, 450, 1247
TspMI CCCGGG 1 cut(s) 1610
TspRI CASTG 8 cut(s) 289, 391, 412, 457, 667, 1081, 1365, 1702
VpaK11BI GGWCC 3 cut(s) 1280, 1537, 1955
VspI ATTAAT 2 cut(s) 888, 1407
XapI RAATTY 4 cut(s) 833, 1003, 1562, 2095
XbaI TCTAGA 2 cut(s) 247, 1274
XceI RCATGY 1 cut(s) 1634
XcmI CCANNNNNNNNNTGG 2 cut(s) 312, 1740
XmaI CCCGGG 1 cut(s) 1610
XmiI GTMKAC 2 cut(s) 558, 1605
XmnI GAANNNNTTC 1 cut(s) 1934
XspI CTAG 7 cut(s) 248, 546, 1070, 1275, 1446, 1772, 1812
ZrmI AGTACT 1 cut(s) 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.