Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g01310 |
| malus_domestica | MD06G1070500.v1.1 |
| prunus_persica | Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1 |
| pyrus_communis | pycom06g07000 pycom06g07030 |
| rosa_chinensis | RchiOBHm_Chr7g0199611 |
| rosa_laevigata | RLG00000003781 |
| rosa_multiflora | Rmu_sc0009191.1_g000006 |
| rosa_roxburghii | Rroxscaffold_3G00256540 |
| rosa_rugosa | Rorug07G0055500 |
| rosa_samantha | Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500 |
| rosa_wichuraiana | Rw7G015850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 440, 1076 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1370, 1558 |
| Acc36I | ACCTGC | 2 cut(s) | 440, 1076 |
| AccB1I | GGYRCC | 4 cut(s) | 419, 562, 1378, 1492 |
| AccBSI | CCGCTC | 1 cut(s) | 10 |
| AciI | CCGC | 3 cut(s) | 10, 457, 2087 |
| AclWI | GGATC | 4 cut(s) | 1884, 1918, 2257, 2270 |
| AcoI | YGGCCR | 2 cut(s) | 347, 1915 |
| AcsI | RAATTY | 3 cut(s) | 942, 1132, 1691 |
| AcuI | CTGAAG | 1 cut(s) | 537 |
| AfaI | GTAC | 4 cut(s) | 76, 635, 1139, 1776 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 308, 621, 1056, 1057, 2007 |
| AgsI | TTSAA | 6 cut(s) | 51, 367, 396, 752, 1910, 2233 |
| AjnI | CCWGG | 7 cut(s) | 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 51, 83 |
| Alw26I | GTCTC | 3 cut(s) | 504, 1352, 1488 |
| AlwI | GGATC | 4 cut(s) | 1884, 1918, 2257, 2270 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1845 |
| AoxI | GGCC | 6 cut(s) | 11, 347, 382, 553, 836, 1915 |
| ApoI | RAATTY | 3 cut(s) | 942, 1132, 1691 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 362, 394 |
| AseI | ATTAAT | 1 cut(s) | 1986 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 76, 2063 |
| AspLEI | GCGC | 1 cut(s) | 737 |
| AspS9I | GGNCC | 6 cut(s) | 383, 836, 1409, 1666, 2084, 2140 |
| AsuC2I | CCSGG | 2 cut(s) | 557, 835 |
| AsuHPI | GGTGA | 7 cut(s) | 421, 1273, 1367, 1841, 1942, 1956, 2161 |
| AsuII | TTCGAA | 1 cut(s) | 1512 |
| AsuNHI | GCTAGC | 1 cut(s) | 583 |
| AvaII | GGWCC | 4 cut(s) | 1409, 1666, 2084, 2140 |
| AxyI | CCTNAGG | 1 cut(s) | 15 |
| BalI | TGGCCA | 2 cut(s) | 349, 1917 |
| BamHI | GGATCC | 1 cut(s) | 2262 |
| BanI | GGYRCC | 4 cut(s) | 419, 562, 1378, 1492 |
| BanII | GRGCYC | 2 cut(s) | 457, 1746 |
| BbsI | GAAGAC | 3 cut(s) | 480, 768, 1521 |
| BccI | CCATC | 9 cut(s) | 698, 1175, 1199, 1286, 1614, 1897, 1907, 2032, 2129 |
| BceAI | ACGGC | 1 cut(s) | 2030 |
| BciT130I | CCWGG | 7 cut(s) | 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| BciVI | GTATCC | 1 cut(s) | 1056 |
| BclI | TGATCA | 1 cut(s) | 1278 |
| BcnI | CCSGG | 2 cut(s) | 557, 835 |
| BcoDI | GTCTC | 3 cut(s) | 504, 1352, 1488 |
| BfaI | CTAG | 4 cut(s) | 332, 584, 621, 957 |
| BfmI | CTRYAG | 5 cut(s) | 103, 303, 549, 666, 1083 |
| BfoI | RGCGCY | 1 cut(s) | 738 |
| BfuAI | ACCTGC | 2 cut(s) | 440, 1076 |
| BfuI | GTATCC | 1 cut(s) | 1056 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1580 |
| BmcAI | AGTACT | 1 cut(s) | 76 |
| Bme1390I | CCNGG | 9 cut(s) | 557, 835, 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| Bme18I | GGWCC | 4 cut(s) | 1409, 1666, 2084, 2140 |
| BmgT120I | GGNCC | 6 cut(s) | 383, 836, 1409, 1666, 2084, 2140 |
| BmrFI | CCNGG | 9 cut(s) | 557, 835, 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| BmsI | GCATC | 5 cut(s) | 659, 682, 1057, 1744, 1766 |
| BmtI | GCTAGC | 1 cut(s) | 587 |
| BpiI | GAAGAC | 3 cut(s) | 480, 768, 1521 |
| BpmI | CTGGAG | 2 cut(s) | 1470, 1990 |
| Bpu14I | TTCGAA | 1 cut(s) | 1512 |
| BpuEI | CTTGAG | 3 cut(s) | 799, 1421, 2234 |
| BpuMI | CCSGG | 2 cut(s) | 557, 835 |
| BsaI | GGTCTC | 1 cut(s) | 1488 |
| BsaJI | CCNNGG | 7 cut(s) | 87, 869, 1456, 1739, 2007, 2182, 2266 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 308, 621, 1056, 1057, 2007 |
| Bse1I | ACTGG | 3 cut(s) | 1334, 1487, 1575 |
| Bse21I | CCTNAGG | 1 cut(s) | 15 |
| BseBI | CCWGG | 7 cut(s) | 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| BseDI | CCNNGG | 7 cut(s) | 87, 869, 1456, 1739, 2007, 2182, 2266 |
| BseGI | GGATG | 5 cut(s) | 662, 1186, 1375, 1606, 2024 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 308, 621, 1056, 1057, 2007 |
| BseMII | CTCAG | 1 cut(s) | 1110 |
| BseNI | ACTGG | 3 cut(s) | 1334, 1487, 1575 |
| BseRI | GAGGAG | 1 cut(s) | 1077 |
| BsgI | GTGCAG | 1 cut(s) | 2139 |
| BshFI | GGCC | 6 cut(s) | 13, 349, 384, 555, 838, 1917 |
| BshNI | GGYRCC | 4 cut(s) | 419, 562, 1378, 1492 |
| BsiSI | CCGG | 2 cut(s) | 556, 834 |
| BslFI | GGGAC | 4 cut(s) | 278, 596, 1020, 1564 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 308, 621, 1056, 1057, 2007 |
| BsmAI | GTCTC | 3 cut(s) | 504, 1352, 1488 |
| BsmBI | CGTCTC | 1 cut(s) | 1352 |
| BsmFI | GGGAC | 4 cut(s) | 278, 596, 1020, 1564 |
| BsmI | GAATGC | 2 cut(s) | 1191, 1250 |
| BsnI | GGCC | 6 cut(s) | 13, 349, 384, 555, 838, 1917 |
| Bso31I | GGTCTC | 1 cut(s) | 1488 |
| Bsp119I | TTCGAA | 1 cut(s) | 1512 |
| Bsp1286I | GDGCHC | 2 cut(s) | 457, 1746 |
| Bsp1407I | TGTACA | 2 cut(s) | 633, 1774 |
| Bsp143I | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| BspACI | CCGC | 3 cut(s) | 10, 457, 2087 |
| BspANI | GGCC | 6 cut(s) | 13, 349, 384, 555, 838, 1917 |
| BspCNI | CTCAG | 1 cut(s) | 1109 |
| BspHI | TCATGA | 1 cut(s) | 1644 |
| BspMAI | CTGCAG | 3 cut(s) | 553, 670, 1087 |
| BspMI | ACCTGC | 2 cut(s) | 440, 1076 |
| BspOI | GCTAGC | 1 cut(s) | 587 |
| BspPI | GGATC | 4 cut(s) | 1884, 1918, 2257, 2270 |
| BspQI | GCTCTTC | 2 cut(s) | 110, 1861 |
| BspT104I | TTCGAA | 1 cut(s) | 1512 |
| BspT107I | GGYRCC | 4 cut(s) | 419, 562, 1378, 1492 |
| BspTNI | GGTCTC | 1 cut(s) | 1488 |
| BsrBI | CCGCTC | 1 cut(s) | 10 |
| BsrGI | TGTACA | 2 cut(s) | 633, 1774 |
| BsrI | ACTGG | 3 cut(s) | 1334, 1487, 1575 |
| BssECI | CCNNGG | 7 cut(s) | 87, 869, 1456, 1739, 2007, 2182, 2266 |
| BssMI | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| BssT1I | CCWWGG | 3 cut(s) | 87, 869, 2266 |
| Bst2UI | CCWGG | 7 cut(s) | 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| Bst4CI | ACNGT | 7 cut(s) | 64, 803, 853, 883, 1276, 1948, 2071 |
| Bst6I | CTCTTC | 2 cut(s) | 110, 1861 |
| BstAUI | TGTACA | 2 cut(s) | 633, 1774 |
| BstBI | TTCGAA | 1 cut(s) | 1512 |
| BstC8I | GCNNGC | 6 cut(s) | 457, 553, 567, 585, 892, 1189 |
| BstDEI | CTNAG | 3 cut(s) | 15, 174, 1096 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 306, 2005 |
| BstF5I | GGATG | 5 cut(s) | 662, 1186, 1375, 1606, 2024 |
| BstH2I | RGCGCY | 1 cut(s) | 738 |
| BstHHI | GCGC | 1 cut(s) | 737 |
| BstKTI | GATC | 7 cut(s) | 102, 1281, 1322, 1447, 1879, 1926, 2265 |
| BstMAI | GTCTC | 3 cut(s) | 504, 1352, 1488 |
| BstMBI | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 545, 596, 626, 1097, 1223, 1580, 1763, 1794, 1848 |
| BstNI | CCWGG | 7 cut(s) | 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| BstSCI | CCNGG | 9 cut(s) | 555, 833, 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| BstSFI | CTRYAG | 5 cut(s) | 103, 303, 549, 666, 1083 |
| BstV2I | GAAGAC | 3 cut(s) | 480, 768, 1521 |
| BstX2I | RGATCY | 3 cut(s) | 1876, 1923, 2262 |
| BstYI | RGATCY | 3 cut(s) | 1876, 1923, 2262 |
| Bsu36I | CCTNAGG | 1 cut(s) | 15 |
| BsuI | GTATCC | 1 cut(s) | 1056 |
| BsuRI | GGCC | 6 cut(s) | 13, 349, 384, 555, 838, 1917 |
| BtsCI | GGATG | 5 cut(s) | 662, 1186, 1375, 1606, 2024 |
| BtsI | GCAGTG | 2 cut(s) | 534, 1449 |
| BtsIMutI | CAGTG | 9 cut(s) | 95, 489, 534, 1239, 1281, 1449, 1494, 1582, 2067 |
| BveI | ACCTGC | 2 cut(s) | 440, 1076 |
| Cac8I | GCNNGC | 6 cut(s) | 457, 553, 567, 585, 892, 1189 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1845 |
| CciI | TCATGA | 1 cut(s) | 1644 |
| CfoI | GCGC | 1 cut(s) | 737 |
| Cfr13I | GGNCC | 6 cut(s) | 383, 836, 1409, 1666, 2084, 2140 |
| Csp6I | GTAC | 4 cut(s) | 75, 634, 1138, 1775 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 712, 747 |
| CviQI | GTAC | 4 cut(s) | 75, 634, 1138, 1775 |
| DdeI | CTNAG | 3 cut(s) | 15, 174, 1096 |
| DpnI | GATC | 7 cut(s) | 101, 1280, 1321, 1446, 1878, 1925, 2264 |
| DpnII | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1370, 1558 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1370, 1558 |
| EaeI | YGGCCR | 2 cut(s) | 347, 1915 |
| Eam1104I | CTCTTC | 2 cut(s) | 110, 1861 |
| EarI | CTCTTC | 2 cut(s) | 110, 1861 |
| EciI | GGCGGA | 1 cut(s) | 2076 |
| Eco130I | CCWWGG | 3 cut(s) | 87, 869, 2266 |
| Eco24I | GRGCYC | 2 cut(s) | 457, 1746 |
| Eco31I | GGTCTC | 1 cut(s) | 1488 |
| Eco32I | GATATC | 2 cut(s) | 673, 1104 |
| Eco47I | GGWCC | 4 cut(s) | 1409, 1666, 2084, 2140 |
| Eco57I | CTGAAG | 1 cut(s) | 537 |
| Eco81I | CCTNAGG | 1 cut(s) | 15 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 306, 2005 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1409 |
| EcoRI | GAATTC | 1 cut(s) | 1132 |
| EcoRII | CCWGG | 7 cut(s) | 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| EcoRV | GATATC | 2 cut(s) | 673, 1104 |
| EcoT14I | CCWWGG | 3 cut(s) | 87, 869, 2266 |
| EcoT22I | ATGCAT | 2 cut(s) | 1759, 1993 |
| EcoT38I | GRGCYC | 2 cut(s) | 457, 1746 |
| ErhI | CCWWGG | 3 cut(s) | 87, 869, 2266 |
| Esp3I | CGTCTC | 1 cut(s) | 1352 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 276, 308, 912, 944 |
| FaqI | GGGAC | 4 cut(s) | 278, 596, 1020, 1564 |
| FauI | CCCGC | 1 cut(s) | 464 |
| FbaI | TGATCA | 1 cut(s) | 1278 |
| FokI | GGATG | 5 cut(s) | 669, 1193, 1382, 1593, 2011 |
| FriOI | GRGCYC | 2 cut(s) | 457, 1746 |
| FspBI | CTAG | 4 cut(s) | 332, 584, 621, 957 |
| GlaI | GCGC | 1 cut(s) | 736 |
| GsuI | CTGGAG | 2 cut(s) | 1470, 1990 |
| HaeII | RGCGCY | 1 cut(s) | 738 |
| HaeIII | GGCC | 6 cut(s) | 13, 349, 384, 555, 838, 1917 |
| HapII | CCGG | 2 cut(s) | 556, 834 |
| HhaI | GCGC | 1 cut(s) | 737 |
| Hin6I | GCGC | 1 cut(s) | 735 |
| HinP1I | GCGC | 1 cut(s) | 735 |
| HincII | GTYRAC | 1 cut(s) | 1507 |
| HindII | GTYRAC | 1 cut(s) | 1507 |
| HindIII | AAGCTT | 3 cut(s) | 242, 1817, 2227 |
| HinfI | GANTC | 8 cut(s) | 92, 342, 353, 512, 609, 1173, 1229, 1330 |
| HpaII | CCGG | 2 cut(s) | 556, 834 |
| HphI | GGTGA | 7 cut(s) | 421, 1273, 1367, 1841, 1942, 1956, 2161 |
| Hpy166II | GTNNAC | 4 cut(s) | 634, 1507, 2067, 2159 |
| Hpy188I | TCNGA | 6 cut(s) | 148, 358, 517, 1319, 1725, 1762 |
| Hpy188III | TCNNGA | 4 cut(s) | 332, 654, 1404, 1645 |
| Hpy8I | GTNNAC | 4 cut(s) | 634, 1507, 2067, 2159 |
| Hpy99I | CGWCG | 1 cut(s) | 374 |
| HpyAV | CCTTC | 7 cut(s) | 200, 302, 397, 402, 512, 1509, 2068 |
| HpyCH4III | ACNGT | 7 cut(s) | 64, 803, 853, 883, 1276, 1948, 2071 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 545, 596, 626, 1097, 1223, 1580, 1763, 1794, 1848 |
| HpyF3I | CTNAG | 3 cut(s) | 15, 174, 1096 |
| HspAI | GCGC | 1 cut(s) | 735 |
| Ksp22I | TGATCA | 1 cut(s) | 1278 |
| Kzo9I | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| LguI | GCTCTTC | 2 cut(s) | 110, 1861 |
| LmnI | GCTCC | 3 cut(s) | 452, 742, 1124 |
| LweI | GCATC | 5 cut(s) | 659, 682, 1057, 1744, 1766 |
| MaeI | CTAG | 4 cut(s) | 332, 584, 621, 957 |
| MaeIII | GTNAC | 4 cut(s) | 572, 1829, 1948, 2017 |
| MalI | GATC | 7 cut(s) | 101, 1280, 1321, 1446, 1878, 1925, 2264 |
| MbiI | CCGCTC | 1 cut(s) | 10 |
| MboI | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| MflI | RGATCY | 3 cut(s) | 1876, 1923, 2262 |
| MhlI | GDGCHC | 2 cut(s) | 457, 1746 |
| MlsI | TGGCCA | 2 cut(s) | 349, 1917 |
| MluCI | AATT | 8 cut(s) | 859, 942, 1021, 1053, 1132, 1218, 1344, 1691 |
| MluNI | TGGCCA | 2 cut(s) | 349, 1917 |
| MlyI | GAGTC | 3 cut(s) | 86, 506, 618 |
| MmeI | TCCRAC | 1 cut(s) | 2226 |
| Mox20I | TGGCCA | 2 cut(s) | 349, 1917 |
| Mph1103I | ATGCAT | 2 cut(s) | 1759, 1993 |
| MroXI | GAANNNNTTC | 2 cut(s) | 76, 2063 |
| MscI | TGGCCA | 2 cut(s) | 349, 1917 |
| MseI | TTAA | 8 cut(s) | 123, 998, 1017, 1032, 1734, 1814, 1881, 1986 |
| MslI | CAYNNNNRTG | 3 cut(s) | 655, 1575, 2256 |
| Msp20I | TGGCCA | 2 cut(s) | 349, 1917 |
| MspA1I | CMGCKG | 2 cut(s) | 1202, 1845 |
| MspI | CCGG | 2 cut(s) | 556, 834 |
| MspR9I | CCNGG | 9 cut(s) | 557, 835, 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| Mva1269I | GAATGC | 2 cut(s) | 1191, 1250 |
| MvaI | CCWGG | 7 cut(s) | 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 545, 596, 626, 1097, 1223, 1580, 1763, 1794, 1848 |
| NciI | CCSGG | 2 cut(s) | 557, 835 |
| NdeII | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| NheI | GCTAGC | 1 cut(s) | 583 |
| NmuCI | GTSAC | 2 cut(s) | 1829, 1948 |
| NsiI | ATGCAT | 2 cut(s) | 1759, 1993 |
| NspV | TTCGAA | 1 cut(s) | 1512 |
| PagI | TCATGA | 1 cut(s) | 1644 |
| PaqCI | CACCTGC | 2 cut(s) | 440, 1076 |
| PciSI | GCTCTTC | 2 cut(s) | 110, 1861 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1686 |
| PctI | GAATGC | 2 cut(s) | 1191, 1250 |
| PdmI | GAANNNNTTC | 2 cut(s) | 76, 2063 |
| PfeI | GAWTC | 5 cut(s) | 342, 353, 1173, 1229, 1330 |
| PfoI | TCCNGGA | 1 cut(s) | 1662 |
| PleI | GAGTC | 3 cut(s) | 86, 506, 617 |
| PpsI | GAGTC | 3 cut(s) | 86, 506, 617 |
| PpuMI | RGGWCCY | 1 cut(s) | 1409 |
| PshBI | ATTAAT | 1 cut(s) | 1986 |
| Psp5II | RGGWCCY | 1 cut(s) | 1409 |
| Psp6I | CCWGG | 7 cut(s) | 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| PspGI | CCWGG | 7 cut(s) | 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| PspPI | GGNCC | 6 cut(s) | 383, 836, 1409, 1666, 2084, 2140 |
| PspPPI | RGGWCCY | 1 cut(s) | 1409 |
| PstI | CTGCAG | 3 cut(s) | 553, 670, 1087 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1845 |
| PsuI | RGATCY | 3 cut(s) | 1876, 1923, 2262 |
| PvuII | CAGCTG | 2 cut(s) | 1202, 1845 |
| RsaI | GTAC | 4 cut(s) | 76, 635, 1139, 1776 |
| RsaNI | GTAC | 4 cut(s) | 75, 634, 1138, 1775 |
| RseI | CAYNNNNRTG | 3 cut(s) | 655, 1575, 2256 |
| SapI | GCTCTTC | 2 cut(s) | 110, 1861 |
| SaqAI | TTAA | 8 cut(s) | 123, 998, 1017, 1032, 1734, 1814, 1881, 1986 |
| Sau3AI | GATC | 7 cut(s) | 99, 1278, 1319, 1444, 1876, 1923, 2262 |
| Sau96I | GGNCC | 6 cut(s) | 383, 836, 1409, 1666, 2084, 2140 |
| ScaI | AGTACT | 1 cut(s) | 76 |
| SchI | GAGTC | 3 cut(s) | 86, 506, 618 |
| ScrFI | CCNGG | 9 cut(s) | 557, 835, 1458, 1522, 1664, 1740, 1863, 2008, 2184 |
| SduI | GDGCHC | 2 cut(s) | 457, 1746 |
| SfaNI | GCATC | 5 cut(s) | 659, 682, 1057, 1744, 1766 |
| SfcI | CTRYAG | 5 cut(s) | 103, 303, 549, 666, 1083 |
| SfuI | TTCGAA | 1 cut(s) | 1512 |
| SinI | GGWCC | 4 cut(s) | 1409, 1666, 2084, 2140 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 655, 1575, 2256 |
| SmlI | CTYRAG | 3 cut(s) | 814, 1436, 2213 |
| SmoI | CTYRAG | 3 cut(s) | 814, 1436, 2213 |
| Sse9I | AATT | 8 cut(s) | 859, 942, 1021, 1053, 1132, 1218, 1344, 1691 |
| SsiI | CCGC | 3 cut(s) | 10, 457, 2087 |
| SspI | AATATT | 1 cut(s) | 755 |
| SspMI | CTAG | 4 cut(s) | 332, 584, 621, 957 |
| StyD4I | CCNGG | 9 cut(s) | 555, 833, 1456, 1520, 1662, 1738, 1861, 2006, 2182 |
| StyI | CCWWGG | 3 cut(s) | 87, 869, 2266 |
| TaaI | ACNGT | 7 cut(s) | 64, 803, 853, 883, 1276, 1948, 2071 |
| TaqI | TCGA | 4 cut(s) | 542, 1512, 1530, 1689 |
| TasI | AATT | 8 cut(s) | 859, 942, 1021, 1053, 1132, 1218, 1344, 1691 |
| TatI | WGTACW | 3 cut(s) | 74, 633, 1774 |
| TauI | GCSGC | 1 cut(s) | 13 |
| TfiI | GAWTC | 5 cut(s) | 342, 353, 1173, 1229, 1330 |
| Tru1I | TTAA | 8 cut(s) | 123, 998, 1017, 1032, 1734, 1814, 1881, 1986 |
| Tru9I | TTAA | 8 cut(s) | 123, 998, 1017, 1032, 1734, 1814, 1881, 1986 |
| TscAI | CASTG | 9 cut(s) | 102, 496, 541, 1246, 1281, 1456, 1494, 1582, 2074 |
| TseFI | GTSAC | 2 cut(s) | 1829, 1948 |
| Tsp45I | GTSAC | 2 cut(s) | 1829, 1948 |
| TspDTI | ATGAA | 6 cut(s) | 165, 259, 809, 1109, 1145, 1698 |
| TspGWI | ACGGA | 3 cut(s) | 1376, 1492, 2268 |
| TspRI | CASTG | 9 cut(s) | 102, 496, 541, 1246, 1281, 1456, 1494, 1582, 2074 |
| VpaK11BI | GGWCC | 4 cut(s) | 1409, 1666, 2084, 2140 |
| VspI | ATTAAT | 1 cut(s) | 1986 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 306, 2005 |
| XapI | RAATTY | 3 cut(s) | 942, 1132, 1691 |
| XbaI | TCTAGA | 1 cut(s) | 331 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 396, 1118, 1869 |
| XmnI | GAANNNNTTC | 2 cut(s) | 76, 2063 |
| XspI | CTAG | 4 cut(s) | 332, 584, 621, 957 |
| ZrmI | AGTACT | 1 cut(s) | 76 |
| Zsp2I | ATGCAT | 2 cut(s) | 1759, 1993 |