Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g01310 |
| malus_domestica | MD06G1070500.v1.1 |
| prunus_persica | Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1 |
| pyrus_communis | pycom06g07000 pycom06g07030 |
| rosa_chinensis | RchiOBHm_Chr7g0199611 |
| rosa_laevigata | RLG00000003781 |
| rosa_multiflora | Rmu_sc0009191.1_g000006 |
| rosa_roxburghii | Rroxscaffold_3G00256540 |
| rosa_rugosa | Rorug07G0055500 |
| rosa_samantha | Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500 |
| rosa_wichuraiana | Rw7G015850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 503 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1570 |
| Acc36I | ACCTGC | 1 cut(s) | 503 |
| AccB1I | GGYRCC | 5 cut(s) | 102, 482, 625, 1390, 1504 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 462 |
| AccBSI | CCGCTC | 1 cut(s) | 100 |
| AccI | GTMKAC | 3 cut(s) | 87, 699, 1746 |
| AccII | CGCG | 1 cut(s) | 621 |
| AciI | CCGC | 8 cut(s) | 100, 520, 619, 953, 1095, 1464, 1508, 2099 |
| AclWI | GGATC | 8 cut(s) | 120, 902, 1896, 1981, 2146, 2159, 2254, 2279 |
| AcoI | YGGCCR | 4 cut(s) | 174, 410, 954, 1927 |
| AcsI | RAATTY | 4 cut(s) | 974, 1144, 1544, 1703 |
| AcuI | CTGAAG | 4 cut(s) | 264, 412, 717, 2260 |
| AfaI | GTAC | 7 cut(s) | 156, 1198, 1228, 1788, 1855, 2213, 2258 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 462, 1068, 1069 |
| AflIII | ACRYGT | 1 cut(s) | 1771 |
| AgsI | TTSAA | 5 cut(s) | 169, 606, 1026, 1828, 1922 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 562 |
| AjnI | CCWGG | 4 cut(s) | 1468, 1532, 1873, 2194 |
| AleI | CACNNNNGTG | 1 cut(s) | 1967 |
| Alw26I | GTCTC | 4 cut(s) | 796, 1364, 1500, 1635 |
| AlwI | GGATC | 8 cut(s) | 120, 902, 1896, 1981, 2146, 2159, 2254, 2279 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1286 |
| Ama87I | CYCGRG | 1 cut(s) | 1751 |
| AoxI | GGCC | 7 cut(s) | 174, 410, 445, 616, 901, 954, 1927 |
| ApoI | RAATTY | 4 cut(s) | 974, 1144, 1544, 1703 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 959, 991, 1688, 1720 |
| AseI | ATTAAT | 1 cut(s) | 1029 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2075 |
| AspLEI | GCGC | 1 cut(s) | 2005 |
| AspS9I | GGNCC | 4 cut(s) | 446, 902, 1421, 2096 |
| AsuC2I | CCSGG | 3 cut(s) | 1561, 1752, 1753 |
| AsuHPI | GGTGA | 5 cut(s) | 484, 1853, 1968, 2075, 2173 |
| AvaI | CYCGRG | 1 cut(s) | 1751 |
| AvaII | GGWCC | 2 cut(s) | 1421, 2096 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1188, 1221 |
| BalI | TGGCCA | 1 cut(s) | 1929 |
| BamHI | GGATCC | 1 cut(s) | 2151 |
| BanI | GGYRCC | 5 cut(s) | 102, 482, 625, 1390, 1504 |
| BanII | GRGCYC | 2 cut(s) | 520, 1141 |
| BbsI | GAAGAC | 2 cut(s) | 267, 1701 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 216, 250 |
| BciT130I | CCWGG | 4 cut(s) | 1470, 1534, 1875, 2196 |
| BcnI | CCSGG | 3 cut(s) | 1561, 1752, 1753 |
| BcoDI | GTCTC | 4 cut(s) | 796, 1364, 1500, 1635 |
| BfaI | CTAG | 7 cut(s) | 395, 687, 1211, 1416, 1587, 1913, 1953 |
| BfmI | CTRYAG | 5 cut(s) | 193, 366, 612, 1012, 1596 |
| BfuAI | ACCTGC | 1 cut(s) | 503 |
| BmcAI | AGTACT | 1 cut(s) | 1855 |
| Bme1390I | CCNGG | 7 cut(s) | 1470, 1534, 1561, 1752, 1753, 1875, 2196 |
| Bme18I | GGWCC | 2 cut(s) | 1421, 2096 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 562 |
| BmeT110I | CYCGRG | 1 cut(s) | 1751 |
| BmgT120I | GGNCC | 4 cut(s) | 446, 902, 1421, 2096 |
| BmrFI | CCNGG | 7 cut(s) | 1470, 1534, 1561, 1752, 1753, 1875, 2196 |
| BmsI | GCATC | 5 cut(s) | 371, 725, 748, 1069, 1756 |
| BpiI | GAAGAC | 2 cut(s) | 267, 1701 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2155, 2187 |
| BpmI | CTGGAG | 2 cut(s) | 1119, 1482 |
| BpuEI | CTTGAG | 2 cut(s) | 1433, 2002 |
| BpuMI | CCSGG | 3 cut(s) | 1561, 1752, 1753 |
| BsaI | GGTCTC | 3 cut(s) | 796, 1500, 1635 |
| BsaJI | CCNNGG | 7 cut(s) | 58, 106, 935, 1468, 1750, 1751, 2194 |
| BsaWI | WCCGGW | 1 cut(s) | 2126 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 351, 363, 366, 381, 393, 396, 1997, 2027 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 462, 1068, 1069 |
| Bse1I | ACTGG | 5 cut(s) | 562, 633, 1499, 1515, 1843 |
| Bse3DI | GCAATG | 2 cut(s) | 834, 1006 |
| BseBI | CCWGG | 4 cut(s) | 1470, 1534, 1875, 2196 |
| BseDI | CCNNGG | 7 cut(s) | 58, 106, 935, 1468, 1750, 1751, 2194 |
| BseGI | GGATG | 5 cut(s) | 244, 558, 728, 1565, 1618 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 462, 1068, 1069 |
| BseMI | GCAATG | 2 cut(s) | 834, 1006 |
| BseMII | CTCAG | 1 cut(s) | 2214 |
| BseNI | ACTGG | 5 cut(s) | 562, 633, 1499, 1515, 1843 |
| BseRI | GAGGAG | 3 cut(s) | 387, 1089, 2005 |
| BseYI | CCCAGC | 1 cut(s) | 711 |
| BsgI | GTGCAG | 3 cut(s) | 1599, 1635, 2187 |
| Bsh1236I | CGCG | 1 cut(s) | 621 |
| BshFI | GGCC | 7 cut(s) | 176, 412, 447, 618, 903, 956, 1929 |
| BshNI | GGYRCC | 5 cut(s) | 102, 482, 625, 1390, 1504 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1751 |
| BsiSI | CCGG | 4 cut(s) | 413, 1560, 1752, 2127 |
| BslFI | GGGAC | 5 cut(s) | 169, 341, 550, 662, 1576 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 462, 1068, 1069 |
| BsmAI | GTCTC | 4 cut(s) | 796, 1364, 1500, 1635 |
| BsmBI | CGTCTC | 1 cut(s) | 1364 |
| BsmFI | GGGAC | 5 cut(s) | 169, 341, 550, 662, 1576 |
| BsmI | GAATGC | 1 cut(s) | 749 |
| BsnI | GGCC | 7 cut(s) | 176, 412, 447, 618, 903, 956, 1929 |
| Bso31I | GGTCTC | 3 cut(s) | 796, 1500, 1635 |
| BsoBI | CYCGRG | 1 cut(s) | 1751 |
| Bsp1286I | GDGCHC | 2 cut(s) | 520, 1141 |
| Bsp1407I | TGTACA | 1 cut(s) | 1786 |
| Bsp19I | CCATGG | 3 cut(s) | 58, 106, 935 |
| BspACI | CCGC | 8 cut(s) | 100, 520, 619, 953, 1095, 1464, 1508, 2099 |
| BspANI | GGCC | 7 cut(s) | 176, 412, 447, 618, 903, 956, 1929 |
| BspCNI | CTCAG | 1 cut(s) | 2215 |
| BspFNI | CGCG | 1 cut(s) | 621 |
| BspHI | TCATGA | 1 cut(s) | 1656 |
| BspMAI | CTGCAG | 2 cut(s) | 616, 1600 |
| BspMI | ACCTGC | 1 cut(s) | 503 |
| BspPI | GGATC | 8 cut(s) | 120, 902, 1896, 1981, 2146, 2159, 2254, 2279 |
| BspQI | GCTCTTC | 2 cut(s) | 200, 1873 |
| BspT107I | GGYRCC | 5 cut(s) | 102, 482, 625, 1390, 1504 |
| BspTNI | GGTCTC | 3 cut(s) | 796, 1500, 1635 |
| BsrBI | CCGCTC | 1 cut(s) | 100 |
| BsrDI | GCAATG | 2 cut(s) | 834, 1006 |
| BsrGI | TGTACA | 1 cut(s) | 1786 |
| BsrI | ACTGG | 5 cut(s) | 562, 633, 1499, 1515, 1843 |
| BssECI | CCNNGG | 7 cut(s) | 58, 106, 935, 1468, 1750, 1751, 2194 |
| BssNAI | GTATAC | 1 cut(s) | 88 |
| BssT1I | CCWWGG | 3 cut(s) | 58, 106, 935 |
| Bst1107I | GTATAC | 1 cut(s) | 88 |
| Bst2UI | CCWGG | 4 cut(s) | 1470, 1534, 1875, 2196 |
| Bst4CI | ACNGT | 5 cut(s) | 533, 949, 1288, 1960, 2083 |
| Bst6I | CTCTTC | 4 cut(s) | 60, 200, 1873, 2059 |
| BstAUI | TGTACA | 1 cut(s) | 1786 |
| BstC8I | GCNNGC | 7 cut(s) | 520, 616, 630, 958, 1120, 1802, 1931 |
| BstDEI | CTNAG | 1 cut(s) | 2223 |
| BstDSI | CCRYGG | 3 cut(s) | 58, 106, 935 |
| BstF5I | GGATG | 5 cut(s) | 244, 558, 728, 1565, 1618 |
| BstFNI | CGCG | 1 cut(s) | 621 |
| BstHHI | GCGC | 1 cut(s) | 2005 |
| BstMAI | GTCTC | 4 cut(s) | 796, 1364, 1500, 1635 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 608, 692, 1592, 1775 |
| BstNI | CCWGG | 4 cut(s) | 1470, 1534, 1875, 2196 |
| BstNSI | RCATGY | 1 cut(s) | 1775 |
| BstSCI | CCNGG | 7 cut(s) | 1468, 1532, 1559, 1750, 1751, 1873, 2194 |
| BstSFI | CTRYAG | 5 cut(s) | 193, 366, 612, 1012, 1596 |
| BstUI | CGCG | 1 cut(s) | 621 |
| BstV2I | GAAGAC | 2 cut(s) | 267, 1701 |
| BstX2I | RGATCY | 4 cut(s) | 1888, 2151, 2246, 2271 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 640, 2267 |
| BstYI | RGATCY | 4 cut(s) | 1888, 2151, 2246, 2271 |
| BstZ17I | GTATAC | 1 cut(s) | 88 |
| BsuRI | GGCC | 7 cut(s) | 176, 412, 447, 618, 903, 956, 1929 |
| BtgI | CCRYGG | 3 cut(s) | 58, 106, 935 |
| BtsCI | GGATG | 5 cut(s) | 244, 558, 728, 1565, 1618 |
| BtsI | GCAGTG | 1 cut(s) | 597 |
| BtsIMutI | CAGTG | 8 cut(s) | 147, 288, 436, 538, 552, 597, 1506, 1836 |
| BveI | ACCTGC | 1 cut(s) | 503 |
| Cac8I | GCNNGC | 7 cut(s) | 520, 616, 630, 958, 1120, 1802, 1931 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1286 |
| CciI | TCATGA | 1 cut(s) | 1656 |
| CfoI | GCGC | 1 cut(s) | 2005 |
| Cfr13I | GGNCC | 4 cut(s) | 446, 902, 1421, 2096 |
| Cfr9I | CCCGGG | 1 cut(s) | 1751 |
| Csp6I | GTAC | 7 cut(s) | 155, 1197, 1227, 1787, 1854, 2212, 2257 |
| CviQI | GTAC | 7 cut(s) | 155, 1197, 1227, 1787, 1854, 2212, 2257 |
| DdeI | CTNAG | 1 cut(s) | 2223 |
| DraI | TTTAAA | 1 cut(s) | 2242 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1570 |
| DriI | GACNNNNNGTC | 1 cut(s) | 562 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1570 |
| EaeI | YGGCCR | 4 cut(s) | 174, 410, 954, 1927 |
| Eam1104I | CTCTTC | 4 cut(s) | 60, 200, 1873, 2059 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 562 |
| EarI | CTCTTC | 4 cut(s) | 60, 200, 1873, 2059 |
| EciI | GGCGGA | 1 cut(s) | 2088 |
| Eco130I | CCWWGG | 3 cut(s) | 58, 106, 935 |
| Eco24I | GRGCYC | 2 cut(s) | 520, 1141 |
| Eco31I | GGTCTC | 3 cut(s) | 796, 1500, 1635 |
| Eco32I | GATATC | 1 cut(s) | 1384 |
| Eco47I | GGWCC | 2 cut(s) | 1421, 2096 |
| Eco57I | CTGAAG | 4 cut(s) | 264, 412, 717, 2260 |
| Eco88I | CYCGRG | 1 cut(s) | 1751 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1421 |
| EcoRI | GAATTC | 2 cut(s) | 1144, 1703 |
| EcoRII | CCWGG | 4 cut(s) | 1468, 1532, 1873, 2194 |
| EcoRV | GATATC | 1 cut(s) | 1384 |
| EcoT14I | CCWWGG | 3 cut(s) | 58, 106, 935 |
| EcoT38I | GRGCYC | 2 cut(s) | 520, 1141 |
| ErhI | CCWWGG | 3 cut(s) | 58, 106, 935 |
| Esp3I | CGTCTC | 1 cut(s) | 1364 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 339, 371, 978, 1010, 2060, 2092 |
| FaqI | GGGAC | 5 cut(s) | 169, 341, 550, 662, 1576 |
| FauI | CCCGC | 1 cut(s) | 527 |
| FblI | GTMKAC | 3 cut(s) | 87, 699, 1746 |
| FokI | GGATG | 5 cut(s) | 251, 545, 735, 1552, 1605 |
| FriOI | GRGCYC | 2 cut(s) | 520, 1141 |
| FspBI | CTAG | 7 cut(s) | 395, 687, 1211, 1416, 1587, 1913, 1953 |
| GlaI | GCGC | 1 cut(s) | 2004 |
| GsaI | CCCAGC | 1 cut(s) | 715 |
| GsuI | CTGGAG | 2 cut(s) | 1119, 1482 |
| HaeIII | GGCC | 7 cut(s) | 176, 412, 447, 618, 903, 956, 1929 |
| HapII | CCGG | 4 cut(s) | 413, 1560, 1752, 2127 |
| HhaI | GCGC | 1 cut(s) | 2005 |
| Hin6I | GCGC | 1 cut(s) | 2003 |
| HinP1I | GCGC | 1 cut(s) | 2003 |
| HincII | GTYRAC | 2 cut(s) | 1477, 2142 |
| HindII | GTYRAC | 2 cut(s) | 1477, 2142 |
| HindIII | AAGCTT | 3 cut(s) | 163, 305, 1120 |
| HinfI | GANTC | 8 cut(s) | 182, 286, 405, 416, 575, 675, 1241, 1342 |
| HpaII | CCGG | 4 cut(s) | 413, 1560, 1752, 2127 |
| HphI | GGTGA | 5 cut(s) | 484, 1853, 1968, 2075, 2173 |
| Hpy166II | GTNNAC | 7 cut(s) | 79, 88, 700, 1019, 1477, 1747, 2142 |
| Hpy188III | TCNNGA | 5 cut(s) | 395, 657, 720, 1416, 1657 |
| Hpy8I | GTNNAC | 7 cut(s) | 79, 88, 700, 1019, 1477, 1747, 2142 |
| Hpy99I | CGWCG | 1 cut(s) | 584 |
| HpyAV | CCTTC | 5 cut(s) | 26, 67, 263, 1521, 2086 |
| HpyCH4III | ACNGT | 5 cut(s) | 533, 949, 1288, 1960, 2083 |
| HpyCH4IV | ACGT | 2 cut(s) | 153, 2206 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 608, 692, 1592, 1775 |
| HpyF3I | CTNAG | 1 cut(s) | 2223 |
| HpySE526I | ACGT | 2 cut(s) | 153, 2206 |
| HspAI | GCGC | 1 cut(s) | 2003 |
| LguI | GCTCTTC | 2 cut(s) | 200, 1873 |
| LmnI | GCTCC | 5 cut(s) | 359, 515, 545, 1100, 1136 |
| LweI | GCATC | 5 cut(s) | 371, 725, 748, 1069, 1756 |
| MaeI | CTAG | 7 cut(s) | 395, 687, 1211, 1416, 1587, 1913, 1953 |
| MaeII | ACGT | 2 cut(s) | 153, 2206 |
| MaeIII | GTNAC | 9 cut(s) | 635, 803, 874, 1291, 1517, 1721, 1808, 1841, 1960 |
| MbiI | CCGCTC | 1 cut(s) | 100 |
| MflI | RGATCY | 4 cut(s) | 1888, 2151, 2246, 2271 |
| MhlI | GDGCHC | 2 cut(s) | 520, 1141 |
| MlsI | TGGCCA | 1 cut(s) | 1929 |
| MluNI | TGGCCA | 1 cut(s) | 1929 |
| MlyI | GAGTC | 2 cut(s) | 191, 684 |
| MmeI | TCCRAC | 3 cut(s) | 216, 237, 603 |
| Mox20I | TGGCCA | 1 cut(s) | 1929 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2075 |
| MscI | TGGCCA | 1 cut(s) | 1929 |
| MseI | TTAA | 8 cut(s) | 213, 824, 1029, 1266, 1548, 1893, 1998, 2241 |
| MslI | CAYNNNNRTG | 5 cut(s) | 721, 1587, 1938, 1967, 2265 |
| Msp20I | TGGCCA | 1 cut(s) | 1929 |
| MspA1I | CMGCKG | 1 cut(s) | 1097 |
| MspI | CCGG | 4 cut(s) | 413, 1560, 1752, 2127 |
| MspR9I | CCNGG | 7 cut(s) | 1470, 1534, 1561, 1752, 1753, 1875, 2196 |
| Mva1269I | GAATGC | 1 cut(s) | 749 |
| MvaI | CCWGG | 4 cut(s) | 1470, 1534, 1875, 2196 |
| MvnI | CGCG | 1 cut(s) | 621 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 608, 692, 1592, 1775 |
| NciI | CCSGG | 3 cut(s) | 1561, 1752, 1753 |
| NcoI | CCATGG | 3 cut(s) | 58, 106, 935 |
| NmuCI | GTSAC | 4 cut(s) | 874, 1721, 1841, 1960 |
| NspI | RCATGY | 1 cut(s) | 1775 |
| OliI | CACNNNNGTG | 1 cut(s) | 1967 |
| PagI | TCATGA | 1 cut(s) | 1656 |
| PaqCI | CACCTGC | 1 cut(s) | 503 |
| PciI | ACATGT | 1 cut(s) | 1771 |
| PciSI | GCTCTTC | 2 cut(s) | 200, 1873 |
| PctI | GAATGC | 1 cut(s) | 749 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2075 |
| PfeI | GAWTC | 6 cut(s) | 286, 405, 416, 575, 1241, 1342 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 462 |
| PfoI | TCCNGGA | 1 cut(s) | 1559 |
| PleI | GAGTC | 2 cut(s) | 190, 683 |
| PpsI | GAGTC | 2 cut(s) | 190, 683 |
| PpuMI | RGGWCCY | 1 cut(s) | 1421 |
| PscI | ACATGT | 1 cut(s) | 1771 |
| PshBI | ATTAAT | 1 cut(s) | 1029 |
| Psp5II | RGGWCCY | 1 cut(s) | 1421 |
| Psp6I | CCWGG | 4 cut(s) | 1468, 1532, 1873, 2194 |
| PspFI | CCCAGC | 1 cut(s) | 711 |
| PspGI | CCWGG | 4 cut(s) | 1468, 1532, 1873, 2194 |
| PspPI | GGNCC | 4 cut(s) | 446, 902, 1421, 2096 |
| PspPPI | RGGWCCY | 1 cut(s) | 1421 |
| PstI | CTGCAG | 2 cut(s) | 616, 1600 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1286 |
| PsuI | RGATCY | 4 cut(s) | 1888, 2151, 2246, 2271 |
| RsaI | GTAC | 7 cut(s) | 156, 1198, 1228, 1788, 1855, 2213, 2258 |
| RsaNI | GTAC | 7 cut(s) | 155, 1197, 1227, 1787, 1854, 2212, 2257 |
| RseI | CAYNNNNRTG | 5 cut(s) | 721, 1587, 1938, 1967, 2265 |
| SapI | GCTCTTC | 2 cut(s) | 200, 1873 |
| SaqAI | TTAA | 8 cut(s) | 213, 824, 1029, 1266, 1548, 1893, 1998, 2241 |
| Sau96I | GGNCC | 4 cut(s) | 446, 902, 1421, 2096 |
| ScaI | AGTACT | 1 cut(s) | 1855 |
| SchI | GAGTC | 2 cut(s) | 191, 684 |
| ScrFI | CCNGG | 7 cut(s) | 1470, 1534, 1561, 1752, 1753, 1875, 2196 |
| SduI | GDGCHC | 2 cut(s) | 520, 1141 |
| SfaNI | GCATC | 5 cut(s) | 371, 725, 748, 1069, 1756 |
| SfcI | CTRYAG | 5 cut(s) | 193, 366, 612, 1012, 1596 |
| SinI | GGWCC | 2 cut(s) | 1421, 2096 |
| SmaI | CCCGGG | 1 cut(s) | 1753 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 721, 1587, 1938, 1967, 2265 |
| SmlI | CTYRAG | 2 cut(s) | 1448, 2017 |
| SmoI | CTYRAG | 2 cut(s) | 1448, 2017 |
| SsiI | CCGC | 8 cut(s) | 100, 520, 619, 953, 1095, 1464, 1508, 2099 |
| SspMI | CTAG | 7 cut(s) | 395, 687, 1211, 1416, 1587, 1913, 1953 |
| StyD4I | CCNGG | 7 cut(s) | 1468, 1532, 1559, 1750, 1751, 1873, 2194 |
| StyI | CCWWGG | 3 cut(s) | 58, 106, 935 |
| TaaI | ACNGT | 5 cut(s) | 533, 949, 1288, 1960, 2083 |
| TaiI | ACGT | 2 cut(s) | 156, 2209 |
| TaqI | TCGA | 4 cut(s) | 124, 753, 1542, 1701 |
| TaqII | GACCGA | 1 cut(s) | 778 |
| TatI | WGTACW | 2 cut(s) | 1786, 1853 |
| TauI | GCSGC | 3 cut(s) | 103, 621, 956 |
| TfiI | GAWTC | 6 cut(s) | 286, 405, 416, 575, 1241, 1342 |
| Tru1I | TTAA | 8 cut(s) | 213, 824, 1029, 1266, 1548, 1893, 1998, 2241 |
| Tru9I | TTAA | 8 cut(s) | 213, 824, 1029, 1266, 1548, 1893, 1998, 2241 |
| TscAI | CASTG | 8 cut(s) | 147, 288, 436, 538, 559, 604, 1506, 1843 |
| TseFI | GTSAC | 4 cut(s) | 874, 1721, 1841, 1960 |
| Tsp45I | GTSAC | 4 cut(s) | 874, 1721, 1841, 1960 |
| TspDTI | ATGAA | 6 cut(s) | 255, 322, 875, 1233, 1334, 1707 |
| TspGWI | ACGGA | 2 cut(s) | 597, 1388 |
| TspMI | CCCGGG | 1 cut(s) | 1751 |
| TspRI | CASTG | 8 cut(s) | 147, 288, 436, 538, 559, 604, 1506, 1843 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 462 |
| VpaK11BI | GGWCC | 2 cut(s) | 1421, 2096 |
| VspI | ATTAAT | 1 cut(s) | 1029 |
| XapI | RAATTY | 4 cut(s) | 974, 1144, 1544, 1703 |
| XbaI | TCTAGA | 2 cut(s) | 394, 1415 |
| XceI | RCATGY | 1 cut(s) | 1775 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 459, 1881 |
| XmaI | CCCGGG | 1 cut(s) | 1751 |
| XmiI | GTMKAC | 3 cut(s) | 87, 699, 1746 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2075 |
| XspI | CTAG | 7 cut(s) | 395, 687, 1211, 1416, 1587, 1913, 1953 |
| ZrmI | AGTACT | 1 cut(s) | 1855 |