Rh7DG183300

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012611
Physical Location & Seq
Forward (+)
17039625 .. 17041498
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG183300.1

Sequence Viewer

Length: 396 bp
ATGGCTAGTGGGTCTCATTTGCTCTATCTTGCCATGGCCAAGAGAGGAAGGTTCACATTGTATACATGGGAGAGCGGCACCATGGAGATTTTTCTGCTCAATCCACACACCATTCAGTGCTACAAAGCCTTCAAGTTGGCCAAAGAGTCGCTGATCTATAGTTATGGGAAGAGCTTTAATGGGTTTTCCGCAAAGTTGTCGGATGAAGAAGTTGCAGAGTTGGCAGAAATGGAAGGTATAGTTTCAGTGATTCCAAACCACAGACTAAAGCTTCACACAACAAGGTCGTGGGACTTCATGGGTTTCAGCAAAGGCCAACGTGGAGCATCTGTAGGAGAAGAGGTTGTGATAGGGCTTCTAGACTCAGGTATTACTAGAGATGAATGCTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

131

Amino Acids

14.6

Weight (kDa)

6.41

Isoelectric Point (pI)

18.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 92 1.4e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 62
AciI CCGC 2 cut(s) 75, 189
AcoI YGGCCR 2 cut(s) 36, 138
AgsI TTSAA 1 cut(s) 133
AluBI AGCT 2 cut(s) 174, 271
AluI AGCT 2 cut(s) 174, 271
Alw26I GTCTC 1 cut(s) 18
AoxI GGCC 3 cut(s) 36, 138, 313
BalI TGGCCA 2 cut(s) 38, 140
BanI GGYRCC 1 cut(s) 77
BcgI CGANNNNNNTGC 2 cut(s) 180, 214
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 3 cut(s) 6, 359, 375
BfmI CTRYAG 2 cut(s) 157, 330
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 79
BmsI GCATC 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 33, 81
BsaXI ACNNNNNCTCC 4 cut(s) 315, 327, 345, 357
BseDI CCNNGG 2 cut(s) 33, 81
BseGI GGATG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 378
BshFI GGCC 3 cut(s) 38, 140, 315
BshNI GGYRCC 1 cut(s) 77
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 3 cut(s) 38, 140, 315
Bso31I GGTCTC 1 cut(s) 18
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 2 cut(s) 33, 81
BspACI CCGC 2 cut(s) 75, 189
BspANI GGCC 3 cut(s) 38, 140, 315
BspCNI CTCAG 1 cut(s) 377
BspLI GGNNCC 1 cut(s) 79
BspQI GCTCTTC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 18
BsrBI CCGCTC 1 cut(s) 75
BssECI CCNNGG 2 cut(s) 33, 81
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 33, 81
Bst1107I GTATAC 1 cut(s) 63
Bst6I CTCTTC 2 cut(s) 164, 333
BstDEI CTNAG 1 cut(s) 364
BstDSI CCRYGG 2 cut(s) 33, 81
BstF5I GGATG 1 cut(s) 208
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstSFI CTRYAG 2 cut(s) 157, 330
BstZ17I GTATAC 1 cut(s) 63
BsuRI GGCC 3 cut(s) 38, 140, 315
BtgI CCRYGG 2 cut(s) 33, 81
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 122, 252
CviAII CATG 4 cut(s) 34, 66, 82, 298
CviJI RGCY 8 cut(s) 5, 38, 128, 140, 174, 271, 315, 355
CviKI_1 RGCY 8 cut(s) 5, 38, 128, 140, 174, 271, 315, 355
DdeI CTNAG 1 cut(s) 364
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 36, 138
Eam1104I CTCTTC 2 cut(s) 164, 333
EarI CTCTTC 2 cut(s) 164, 333
Eco130I CCWWGG 2 cut(s) 33, 81
Eco31I GGTCTC 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 33, 81
ErhI CCWWGG 2 cut(s) 33, 81
FaeI CATG 4 cut(s) 37, 69, 85, 301
FaiI YATR 9 cut(s) 35, 63, 67, 83, 159, 165, 239, 299, 394
FaqI GGGAC 1 cut(s) 305
FatI CATG 4 cut(s) 33, 65, 81, 297
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 3 cut(s) 6, 359, 375
GluI GCNGC 1 cut(s) 76
HaeIII GGCC 3 cut(s) 38, 140, 315
Hin1II CATG 4 cut(s) 37, 69, 85, 301
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 3 cut(s) 146, 250, 362
Hpy166II GTNNAC 2 cut(s) 54, 63
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 54, 63
HpyAV CCTTC 3 cut(s) 42, 139, 227
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 1 cut(s) 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 1 cut(s) 364
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 4 cut(s) 37, 69, 85, 301
Kzo9I GATC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 1 cut(s) 351
LweI GCATC 1 cut(s) 335
MaeI CTAG 3 cut(s) 6, 359, 375
MaeII ACGT 1 cut(s) 319
MalI GATC 1 cut(s) 155
MbiI CCGCTC 1 cut(s) 75
MboI GATC 1 cut(s) 153
MboII GAAGA 3 cut(s) 181, 218, 350
MlsI TGGCCA 2 cut(s) 38, 140
MluNI TGGCCA 2 cut(s) 38, 140
MlyI GAGTC 2 cut(s) 155, 356
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 2 cut(s) 38, 334
Mox20I TGGCCA 2 cut(s) 38, 140
MscI TGGCCA 2 cut(s) 38, 140
MseI TTAA 1 cut(s) 177
Msp20I TGGCCA 2 cut(s) 38, 140
Mva1269I GAATGC 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 221
NcoI CCATGG 2 cut(s) 33, 81
NdeII GATC 1 cut(s) 153
NlaIII CATG 4 cut(s) 37, 69, 85, 301
NlaIV GGNNCC 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 2 cut(s) 154, 356
PpsI GAGTC 2 cut(s) 154, 356
PspN4I GGNNCC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 1 cut(s) 153
SchI GAGTC 2 cut(s) 155, 356
SetI ASST 8 cut(s) 53, 176, 238, 273, 287, 322, 345, 370
SfaNI GCATC 1 cut(s) 335
SfcI CTRYAG 2 cut(s) 157, 330
SsiI CCGC 2 cut(s) 75, 189
SspMI CTAG 3 cut(s) 6, 359, 375
StyI CCWWGG 2 cut(s) 33, 81
TaiI ACGT 1 cut(s) 322
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 2 cut(s) 122, 252
TspDTI ATGAA 3 cut(s) 219, 286, 396
TspRI CASTG 2 cut(s) 122, 252
XbaI TCTAGA 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 62
XspI CTAG 3 cut(s) 6, 359, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.