Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005143

Physical Location & Seq

Genomic Coordinates Forward (+)

19070147 .. 19074733

Gene Structure 4587 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1076900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGGCGTTGAAAGAGAGTTTAAATAGGTTCAAGAAGCAGCAAGAGAGCGCACAGTCACTTCTTATTGGCATTGCATCTGATAGGGCCACAGCAACACCAAGACCTGCGCCTGCGGTCACTAAGGCTCCTGCACCAGCTGTCAAATTTTCAAATGATACGGAGAGACTTCAACATATTAATAGCATACGGAAAGCCCCTGTTGGAGCTCAGATCAAGCGTGTTATAGACCTGTTGCGGGAGACAAGGCTTTCGTATACACCAGAGCAAATTAATCAAGAATGTTATGTAGATGTGAATGCTAACAAAGCTGTCTTTGACAGTCTGAGGAATAACCTAAAAGTTTCTTATGATGGGAAGAGCTTCTCTTATAAGTCCAAACATCGTATCATGGACAAAAAACAGCTTCTGTCCCTAGTACGGAAATTTTCAGAGGGTATACCGGTCATTGATCTCAAGGATGCTTACCCAAATGTGATGGACGATTTACAGGCTTTGAAAGCTTCGGGTGATATCTGGCTGCTTTCAAACTTTGATTCACAAGAGGACATAGCATATCCAAACGACCCCAGGTTGCCTGCCTCCAAGGTTGACGATGACCTTAAGGTGCTCTTTAGGGGGATTGAGTTGCCGCGTGATATGATTGACATTGAGAAAGAGCTCCAGAAGAATGGGATGAAGCCGGCCACCGATACTGCAAAAAGAAGGGCCTTGGCACAGAGTCAAGGCATTCCTAGCAAATCCAAACCCAAGAAGAAGAAGCACGAGATCACAAAGAGGACCAAGCTTACAAACGCCCATCTGCCGGAGCTTTTCCAAAACCTAAAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005673 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIE_beta

Protein Analysis

277

Amino Acids

31.16

Weight (kDa)

9.72

Isoelectric Point (pI)

46.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIE_beta	PF02186	70 - 129	4e-06	TFIIE beta subunit core domain
TFA2_Winged_2	PF18121	131 - 187	1.3e-17	TFA2 Winged helix domain 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20330
fragaria_vesca	FvH4_3g33500 FvH4_5g01870
malus_domestica	MD06G1076900.v1.1 MD14G1098300.v1.1
prunus_persica	Prupe.5G094900_v2.0.a1 Prupe.5G094900_v2.0.a1 Prupe.5G094900_v2.0.a1
pyrus_communis	pycom06g07450 pycom14g09650
rosa_chinensis	RchiOBHm_Chr5g0060921 RchiOBHm_Chr7g0198651
rosa_laevigata	RLG00000003851 RLG00000035413
rosa_multiflora	Rmu_sc0000420.1_g000016 Rmu_sc0009374.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019720 Rroxscaffold_3G00257560
rosa_rugosa	Rorug05G0334900 Rorug05G0335000 Rorug07G0048900
rosa_samantha	Rh5AG397900 Rh5BG410500 Rh5CG435700 Rh5DG425100 Rh7AG172500 Rh7BG176000 Rh7CG181900
rosa_wichuraiana	Rw5G037520 Rw7G015140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	369
Acc36I	ACCTGC	1 cut(s)	112
AccI	GTMKAC	2 cut(s)	254, 436
AccII	CGCG	1 cut(s)	631
AciI	CCGC	3 cut(s)	113, 235, 629
AcoI	YGGCCR	1 cut(s)	681
AcsI	RAATTY	2 cut(s)	143, 422
AfaI	GTAC	1 cut(s)	417
AfiI	CCNNNNNNNGG	3 cut(s)	235, 417, 802
AflII	CTTAAG	1 cut(s)	599
AgeI	ACCGGT	1 cut(s)	439
AgsI	TTSAA	6 cut(s)	10, 31, 150, 170, 496, 525
AjnI	CCWGG	1 cut(s)	566
AluBI	AGCT	9 cut(s)	137, 206, 308, 360, 403, 500, 658, 784, 808
AluI	AGCT	9 cut(s)	137, 206, 308, 360, 403, 500, 658, 784, 808
Alw21I	GWGCWC	3 cut(s)	208, 609, 660
Alw26I	GTCTC	2 cut(s)	157, 233
AlwNI	CAGNNNCTG	1 cut(s)	406
AoxI	GGCC	3 cut(s)	84, 681, 705
ApeKI	GCWGC	2 cut(s)	37, 517
ApoI	RAATTY	2 cut(s)	143, 422
ArsI	GACNNNNNNTTYG	2 cut(s)	232, 264
AseI	ATTAAT	2 cut(s)	177, 270
AsiGI	ACCGGT	1 cut(s)	439
Asp700I	GAANNNNTTC	1 cut(s)	359
AspLEI	GCGC	2 cut(s)	50, 109
AspS9I	GGNCC	3 cut(s)	84, 705, 777
AsuHPI	GGTGA	1 cut(s)	518
AvaII	GGWCC	1 cut(s)	777
BanII	GRGCYC	2 cut(s)	208, 660
BauI	CACGAG	1 cut(s)	761
Bbv12I	GWGCWC	3 cut(s)	208, 609, 660
BbvI	GCAGC	2 cut(s)	49, 504
BccI	CCATC	3 cut(s)	344, 469, 804
BciT130I	CCWGG	1 cut(s)	568
BcoDI	GTCTC	2 cut(s)	157, 233
BfaI	CTAG	3 cut(s)	413, 732, 829
BfrI	CTTAAG	1 cut(s)	599
BfuAI	ACCTGC	1 cut(s)	112
BisI	GCNGC	3 cut(s)	38, 518, 629
BlsI	GCNGC	3 cut(s)	39, 519, 630
Bme1390I	CCNGG	1 cut(s)	568
Bme18I	GGWCC	1 cut(s)	777
BmgT120I	GGNCC	3 cut(s)	84, 705, 777
BmiI	GGNNCC	1 cut(s)	126
BmrFI	CCNGG	1 cut(s)	568
BmsI	GCATC	2 cut(s)	83, 448
BpmI	CTGGAG	1 cut(s)	644
BpuEI	CTTGAG	1 cut(s)	437
BsaJI	CCNNGG	3 cut(s)	566, 582, 708
BsaWI	WCCGGW	1 cut(s)	439
BsaXI	ACNNNNNCTCC	2 cut(s)	109, 139
Bsc4I	CCNNNNNNNGG	3 cut(s)	235, 417, 802
Bse118I	RCCGGY	2 cut(s)	439, 679
Bse3DI	GCAATG	1 cut(s)	69
BseBI	CCWGG	1 cut(s)	568
BseDI	CCNNGG	3 cut(s)	566, 582, 708
BseGI	GGATG	2 cut(s)	463, 678
BseLI	CCNNNNNNNGG	3 cut(s)	235, 417, 802
BseMI	GCAATG	1 cut(s)	69
BseMII	CTCAG	2 cut(s)	221, 314
BseXI	GCAGC	2 cut(s)	49, 504
BsgI	GTGCAG	1 cut(s)	114
Bsh1236I	CGCG	1 cut(s)	631
BshFI	GGCC	3 cut(s)	86, 683, 707
BshTI	ACCGGT	1 cut(s)	439
BsiHKAI	GWGCWC	3 cut(s)	208, 609, 660
BsiSI	CCGG	3 cut(s)	440, 680, 803
BslFI	GGGAC	1 cut(s)	394
BslI	CCNNNNNNNGG	3 cut(s)	235, 417, 802
BsmAI	GTCTC	2 cut(s)	157, 233
BsmFI	GGGAC	1 cut(s)	394
BsmI	GAATGC	2 cut(s)	301, 726
BsnI	GGCC	3 cut(s)	86, 683, 707
Bsp1286I	GDGCHC	3 cut(s)	208, 609, 660
Bsp143I	GATC	3 cut(s)	210, 448, 765
BspACI	CCGC	3 cut(s)	113, 235, 629
BspANI	GGCC	3 cut(s)	86, 683, 707
BspCNI	CTCAG	2 cut(s)	220, 315
BspFNI	CGCG	1 cut(s)	631
BspLI	GGNNCC	1 cut(s)	126
BspMI	ACCTGC	1 cut(s)	112
BspQI	GCTCTTC	1 cut(s)	350
BspTI	CTTAAG	1 cut(s)	599
BsrDI	GCAATG	1 cut(s)	69
BsrFI	RCCGGY	2 cut(s)	439, 679
BssAI	RCCGGY	2 cut(s)	439, 679
BssECI	CCNNGG	3 cut(s)	566, 582, 708
BssMI	GATC	3 cut(s)	210, 448, 765
BssNAI	GTATAC	2 cut(s)	255, 437
BssSI	CACGAG	1 cut(s)	761
BssT1I	CCWWGG	2 cut(s)	582, 708
Bst1107I	GTATAC	2 cut(s)	255, 437
Bst2BI	CACGAG	1 cut(s)	761
Bst2UI	CCWGG	1 cut(s)	568
Bst4CI	ACNGT	2 cut(s)	54, 320
Bst6I	CTCTTC	1 cut(s)	350
BstAFI	CTTAAG	1 cut(s)	599
BstC8I	GCNNGC	3 cut(s)	111, 576, 681
BstDEI	CTNAG	3 cut(s)	120, 207, 323
BstF5I	GGATG	2 cut(s)	463, 678
BstFNI	CGCG	1 cut(s)	631
BstHHI	GCGC	2 cut(s)	50, 109
BstKTI	GATC	3 cut(s)	213, 451, 768
BstMAI	GTCTC	2 cut(s)	157, 233
BstMBI	GATC	3 cut(s)	210, 448, 765
BstMWI	GCNNNNNNNGC	3 cut(s)	305, 497, 732
BstNI	CCWGG	1 cut(s)	568
BstSCI	CCNGG	1 cut(s)	566
BstUI	CGCG	1 cut(s)	631
BstV1I	GCAGC	2 cut(s)	49, 504
BstXI	CCANNNNNNTGG	1 cut(s)	668
BstZ17I	GTATAC	2 cut(s)	255, 437
BsuRI	GGCC	3 cut(s)	86, 683, 707
BtsCI	GGATG	2 cut(s)	463, 678
BveI	ACCTGC	1 cut(s)	112
Cac8I	GCNNGC	3 cut(s)	111, 576, 681
CaiI	CAGNNNCTG	1 cut(s)	406
CfoI	GCGC	2 cut(s)	50, 109
Cfr10I	RCCGGY	2 cut(s)	439, 679
Cfr13I	GGNCC	3 cut(s)	84, 705, 777
Csp6I	GTAC	1 cut(s)	416
CspAI	ACCGGT	1 cut(s)	439
CviAII	CATG	1 cut(s)	388
CviQI	GTAC	1 cut(s)	416
DdeI	CTNAG	3 cut(s)	120, 207, 323
DpnI	GATC	3 cut(s)	212, 450, 767
DpnII	GATC	3 cut(s)	210, 448, 765
DraI	TTTAAA	1 cut(s)	21
EaeI	YGGCCR	1 cut(s)	681
Eam1104I	CTCTTC	1 cut(s)	350
EarI	CTCTTC	1 cut(s)	350
Ecl136II	GAGCTC	2 cut(s)	206, 658
Eco130I	CCWWGG	2 cut(s)	582, 708
Eco24I	GRGCYC	2 cut(s)	208, 660
Eco32I	GATATC	1 cut(s)	511
Eco47I	GGWCC	1 cut(s)	777
Eco53kI	GAGCTC	2 cut(s)	206, 658
EcoICRI	GAGCTC	2 cut(s)	206, 658
EcoO109I	RGGNCCY	1 cut(s)	705
EcoRII	CCWGG	1 cut(s)	566
EcoRV	GATATC	1 cut(s)	511
EcoT14I	CCWWGG	2 cut(s)	582, 708
EcoT38I	GRGCYC	2 cut(s)	208, 660
ErhI	CCWWGG	2 cut(s)	582, 708
FaeI	CATG	1 cut(s)	391
FalI	AAGNNNNNCTT	2 cut(s)	593, 625
FaqI	GGGAC	1 cut(s)	394
FatI	CATG	1 cut(s)	387
FauI	CCCGC	1 cut(s)	228
FblI	GTMKAC	2 cut(s)	254, 436
Fnu4HI	GCNGC	3 cut(s)	38, 518, 629
FokI	GGATG	2 cut(s)	470, 685
FriOI	GRGCYC	2 cut(s)	208, 660
Fsp4HI	GCNGC	3 cut(s)	38, 518, 629
FspBI	CTAG	3 cut(s)	413, 732, 829
GlaI	GCGC	2 cut(s)	49, 108
GluI	GCNGC	3 cut(s)	38, 518, 629
GsuI	CTGGAG	1 cut(s)	644
HaeIII	GGCC	3 cut(s)	86, 683, 707
HapII	CCGG	3 cut(s)	440, 680, 803
HhaI	GCGC	2 cut(s)	50, 109
Hin1II	CATG	1 cut(s)	391
Hin6I	GCGC	2 cut(s)	48, 107
HinP1I	GCGC	2 cut(s)	48, 107
HincII	GTYRAC	1 cut(s)	589
HindII	GTYRAC	1 cut(s)	589
HindIII	AAGCTT	2 cut(s)	498, 782
HinfI	GANTC	2 cut(s)	533, 718
HpaII	CCGG	3 cut(s)	440, 680, 803
HphI	GGTGA	1 cut(s)	518
Hpy166II	GTNNAC	3 cut(s)	255, 437, 589
Hpy188I	TCNGA	4 cut(s)	79, 210, 324, 430
Hpy188III	TCNNGA	3 cut(s)	31, 275, 661
Hpy8I	GTNNAC	3 cut(s)	255, 437, 589
HpyAV	CCTTC	1 cut(s)	696
HpyCH4III	ACNGT	2 cut(s)	54, 320
HpyCH4V	TGCA	3 cut(s)	74, 131, 695
HpyF10VI	GCNNNNNNNGC	3 cut(s)	305, 497, 732
HpyF3I	CTNAG	3 cut(s)	120, 207, 323
Hsp92II	CATG	1 cut(s)	391
HspAI	GCGC	2 cut(s)	48, 107
KroI	GCCGGC	1 cut(s)	679
KroNI	GCCGGC	1 cut(s)	681
Kzo9I	GATC	3 cut(s)	210, 448, 765
LguI	GCTCTTC	1 cut(s)	350
LmnI	GCTCC	4 cut(s)	130, 203, 663, 805
Lsp1109I	GCAGC	2 cut(s)	49, 504
LweI	GCATC	2 cut(s)	83, 448
MaeI	CTAG	3 cut(s)	413, 732, 829
MaeIII	GTNAC	2 cut(s)	54, 115
MalI	GATC	3 cut(s)	212, 450, 767
MboI	GATC	3 cut(s)	210, 448, 765
MboII	GAAGA	4 cut(s)	367, 676, 763, 766
MhlI	GDGCHC	3 cut(s)	208, 609, 660
MluCI	AATT	3 cut(s)	143, 267, 422
MlyI	GAGTC	1 cut(s)	727
MmeI	TCCRAC	1 cut(s)	181
MnlI	CCTC	5 cut(s)	318, 424, 535, 589, 768
MroNI	GCCGGC	1 cut(s)	679
MroXI	GAANNNNTTC	1 cut(s)	359
MseI	TTAA	4 cut(s)	20, 177, 270, 600
MspA1I	CMGCKG	1 cut(s)	137
MspCI	CTTAAG	1 cut(s)	599
MspI	CCGG	3 cut(s)	440, 680, 803
MspR9I	CCNGG	1 cut(s)	568
Mva1269I	GAATGC	2 cut(s)	301, 726
MvaI	CCWGG	1 cut(s)	568
MvnI	CGCG	1 cut(s)	631
MwoI	GCNNNNNNNGC	3 cut(s)	305, 497, 732
NaeI	GCCGGC	1 cut(s)	681
NdeII	GATC	3 cut(s)	210, 448, 765
NgoMIV	GCCGGC	1 cut(s)	679
NlaIII	CATG	1 cut(s)	391
NlaIV	GGNNCC	1 cut(s)	126
NmuCI	GTSAC	2 cut(s)	54, 115
PciSI	GCTCTTC	1 cut(s)	350
PctI	GAATGC	2 cut(s)	301, 726
PdiI	GCCGGC	1 cut(s)	681
PdmI	GAANNNNTTC	1 cut(s)	359
PfeI	GAWTC	1 cut(s)	533
PinAI	ACCGGT	1 cut(s)	439
PkrI	GCNGC	3 cut(s)	39, 519, 630
PleI	GAGTC	1 cut(s)	726
PpsI	GAGTC	1 cut(s)	726
PshBI	ATTAAT	2 cut(s)	177, 270
PsiI	TTATAA	1 cut(s)	369
Psp124BI	GAGCTC	2 cut(s)	208, 660
Psp6I	CCWGG	1 cut(s)	566
PspGI	CCWGG	1 cut(s)	566
PspN4I	GGNNCC	1 cut(s)	126
PspPI	GGNCC	3 cut(s)	84, 705, 777
PstNI	CAGNNNCTG	1 cut(s)	406
PvuII	CAGCTG	1 cut(s)	137
RsaI	GTAC	1 cut(s)	417
RsaNI	GTAC	1 cut(s)	416
SacI	GAGCTC	2 cut(s)	208, 660
SapI	GCTCTTC	1 cut(s)	350
SaqAI	TTAA	4 cut(s)	20, 177, 270, 600
SatI	GCNGC	3 cut(s)	38, 518, 629
Sau3AI	GATC	3 cut(s)	210, 448, 765
Sau96I	GGNCC	3 cut(s)	84, 705, 777
SchI	GAGTC	1 cut(s)	727
ScrFI	CCNGG	1 cut(s)	568
SduI	GDGCHC	3 cut(s)	208, 609, 660
SfaNI	GCATC	2 cut(s)	83, 448
SinI	GGWCC	1 cut(s)	777
SmlI	CTYRAG	2 cut(s)	452, 599
SmoI	CTYRAG	2 cut(s)	452, 599
Sse9I	AATT	3 cut(s)	143, 267, 422
SsiI	CCGC	3 cut(s)	113, 235, 629
SspMI	CTAG	3 cut(s)	413, 732, 829
SstI	GAGCTC	2 cut(s)	208, 660
StyD4I	CCNGG	1 cut(s)	566
StyI	CCWWGG	2 cut(s)	582, 708
TaaI	ACNGT	2 cut(s)	54, 320
TasI	AATT	3 cut(s)	143, 267, 422
TauI	GCSGC	1 cut(s)	631
TfiI	GAWTC	1 cut(s)	533
Tru1I	TTAA	4 cut(s)	20, 177, 270, 600
Tru9I	TTAA	4 cut(s)	20, 177, 270, 600
TseFI	GTSAC	2 cut(s)	54, 115
TseI	GCWGC	2 cut(s)	37, 517
Tsp45I	GTSAC	2 cut(s)	54, 115
TspDTI	ATGAA	1 cut(s)	689
TspGWI	ACGGA	3 cut(s)	173, 202, 433
Vha464I	CTTAAG	1 cut(s)	599
VpaK11BI	GGWCC	1 cut(s)	777
VspI	ATTAAT	2 cut(s)	177, 270
XapI	RAATTY	2 cut(s)	143, 422
XmiI	GTMKAC	2 cut(s)	254, 436
XmnI	GAANNNNTTC	1 cut(s)	359
XspI	CTAG	3 cut(s)	413, 732, 829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.