Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0005143

Physical Location & Seq

Genomic Coordinates Forward (+)

12100968 .. 12105443

Gene Structure 4476 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g07450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGAGGTTACACTTGCCCCATAGGATTTGGATCCTTAAAACTCTGTCCCTCCTCTTTTCTCTCGTCGCTTTAGTAAGCATCCGATCCCCCCATTTGTCCTCTCATATCTCTTACAGTAGCAGCCTTCATCCGAGTCCTCTCGAGCTGTCTTCGATGGCGTTGAAAGAGAGTTTGAATAGGTTCAAGAAGCAGCAAGAGAGCGCACAGTCACTTCTTATCGGCATTGCATCTGATAGGGCCACAGCAACACCAAGACCTGCGCCTGCAGTCACTAAGGCTCCAGCTCCAGCTGTCAAATTTTCAAATGATACGGAGAGACTTCAACATATTAATAGCATACGGAAAGCCCCTGTTGGAGCTCAGATCAAGCGTGTTATAGACCTGTTGCGGGAGACAAGGCTTTCGTATACACCAGAGCAAATTAATCAAGAATGTTATGTCGATGTGAATGCTAACAAAGCTGTCTTTGACAGTCTGAGGAATAACCTAAAAGTTTCTTATGATGGGAAGAGCTTCTCTTATAAGTCCAAACATCGTATCATGGACAAAAAACAGCTTCTGTCCCTAGTACGGAAATTTTCAGAGGGTATACCGGTCATTGATCTCAAGGATGCTTACCCAAATGTGATGGACGATTTACAGGCTTTGAAAGCTTCGGGTGATATCTGGCTGCTTTCAAACTTTGATTCACAAGAGGACATAGCATATCCAAACGACCCCAGGTTGCCTGCCTCCAAGGTTGACGATGACCTTAAGGTGCTCTTTAGGGGGATTGAGTTGCCGCGTGATATGATTGACATTGAGAAAGAGCTCCAGAAGAATGGGATGAAGCCGGCCACGGATACTGCAAAAAGAAGGGCCTTGGCACAGAGTCAAGGCATTCCTAGCAAATCCAAACCCAAGAAGAAGAAGCACGAGATCACAAAGAGGACCAAGCTTACAAACGCCCATCTGCCGGAGCTTTTCCAAAACCTAAAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005673 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIE_beta

Protein Analysis

328

Amino Acids

36.9

Weight (kDa)

9.82

Isoelectric Point (pI)

49.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIE_beta	PF02186	121 - 180	5.2e-06	TFIIE beta subunit core domain
TFA2_Winged_2	PF18121	182 - 238	1.7e-17	TFA2 Winged helix domain 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20330
fragaria_vesca	FvH4_3g33500 FvH4_5g01870
malus_domestica	MD06G1076900.v1.1 MD14G1098300.v1.1
prunus_persica	Prupe.5G094900_v2.0.a1 Prupe.5G094900_v2.0.a1 Prupe.5G094900_v2.0.a1
pyrus_communis	pycom06g07450 pycom14g09650
rosa_chinensis	RchiOBHm_Chr5g0060921 RchiOBHm_Chr7g0198651
rosa_laevigata	RLG00000003851 RLG00000035413
rosa_multiflora	Rmu_sc0000420.1_g000016 Rmu_sc0009374.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019720 Rroxscaffold_3G00257560
rosa_rugosa	Rorug05G0334900 Rorug05G0335000 Rorug07G0048900
rosa_samantha	Rh5AG397900 Rh5BG410500 Rh5CG435700 Rh5DG425100 Rh7AG172500 Rh7BG176000 Rh7CG181900
rosa_wichuraiana	Rw5G037520 Rw7G015140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	522
Acc36I	ACCTGC	1 cut(s)	265
AccI	GTMKAC	2 cut(s)	407, 589
AccII	CGCG	1 cut(s)	784
AciI	CCGC	2 cut(s)	388, 782
AclWI	GGATC	3 cut(s)	25, 38, 78
AcoI	YGGCCR	1 cut(s)	834
AcsI	RAATTY	2 cut(s)	296, 575
AfaI	GTAC	1 cut(s)	570
AfiI	CCNNNNNNNGG	3 cut(s)	388, 570, 955
AflII	CTTAAG	1 cut(s)	752
AgeI	ACCGGT	1 cut(s)	592
AgsI	TTSAA	7 cut(s)	163, 175, 184, 303, 323, 649, 678
AjnI	CCWGG	1 cut(s)	719
Alw21I	GWGCWC	3 cut(s)	361, 762, 813
Alw26I	GTCTC	2 cut(s)	310, 386
AlwI	GGATC	3 cut(s)	25, 38, 78
AlwNI	CAGNNNCTG	1 cut(s)	559
Ama87I	CYCGRG	1 cut(s)	140
AoxI	GGCC	3 cut(s)	237, 834, 858
ApeKI	GCWGC	3 cut(s)	120, 190, 670
ApoI	RAATTY	2 cut(s)	296, 575
ArsI	GACNNNNNNTTYG	2 cut(s)	385, 417
AseI	ATTAAT	2 cut(s)	330, 423
AsiGI	ACCGGT	1 cut(s)	592
Asp700I	GAANNNNTTC	2 cut(s)	179, 512
AspLEI	GCGC	2 cut(s)	203, 262
AspS9I	GGNCC	3 cut(s)	237, 858, 930
AsuHPI	GGTGA	1 cut(s)	671
AvaI	CYCGRG	1 cut(s)	140
AvaII	GGWCC	1 cut(s)	930
BamHI	GGATCC	1 cut(s)	30
BanII	GRGCYC	2 cut(s)	361, 813
BauI	CACGAG	1 cut(s)	914
BbsI	GAAGAC	1 cut(s)	141
Bbv12I	GWGCWC	3 cut(s)	361, 762, 813
BbvI	GCAGC	3 cut(s)	132, 202, 657
BccI	CCATC	4 cut(s)	148, 497, 622, 957
BcgI	CGANNNNNNTGC	2 cut(s)	431, 465
BciT130I	CCWGG	1 cut(s)	721
BciVI	GTATCC	1 cut(s)	835
BcoDI	GTCTC	2 cut(s)	310, 386
BfaI	CTAG	3 cut(s)	566, 885, 982
BfmI	CTRYAG	1 cut(s)	264
BfrI	CTTAAG	1 cut(s)	752
BfuAI	ACCTGC	1 cut(s)	265
BfuI	GTATCC	1 cut(s)	835
BisI	GCNGC	4 cut(s)	121, 191, 671, 782
BlsI	GCNGC	4 cut(s)	122, 192, 672, 783
Bme1390I	CCNGG	1 cut(s)	721
Bme18I	GGWCC	1 cut(s)	930
BmeT110I	CYCGRG	1 cut(s)	140
BmgT120I	GGNCC	3 cut(s)	237, 858, 930
BmiI	GGNNCC	2 cut(s)	32, 279
BmrFI	CCNGG	1 cut(s)	721
BmsI	GCATC	3 cut(s)	87, 236, 601
BpiI	GAAGAC	1 cut(s)	141
BpmI	CTGGAG	3 cut(s)	264, 270, 797
BpuEI	CTTGAG	1 cut(s)	590
BsaBI	GATNNNNATC	1 cut(s)	29
BsaJI	CCNNGG	4 cut(s)	719, 735, 837, 861
BsaWI	WCCGGW	1 cut(s)	592
BsaXI	ACNNNNNCTCC	2 cut(s)	262, 292
Bsc4I	CCNNNNNNNGG	3 cut(s)	388, 570, 955
Bse118I	RCCGGY	2 cut(s)	592, 832
Bse3DI	GCAATG	1 cut(s)	222
Bse8I	GATNNNNATC	1 cut(s)	29
BseBI	CCWGG	1 cut(s)	721
BseDI	CCNNGG	4 cut(s)	719, 735, 837, 861
BseGI	GGATG	4 cut(s)	78, 127, 616, 831
BseJI	GATNNNNATC	1 cut(s)	29
BseLI	CCNNNNNNNGG	3 cut(s)	388, 570, 955
BseMI	GCAATG	1 cut(s)	222
BseMII	CTCAG	2 cut(s)	374, 467
BseRI	GAGGAG	1 cut(s)	41
BseXI	GCAGC	3 cut(s)	132, 202, 657
Bsh1236I	CGCG	1 cut(s)	784
BshFI	GGCC	3 cut(s)	239, 836, 860
BshTI	ACCGGT	1 cut(s)	592
BsiHKAI	GWGCWC	3 cut(s)	361, 762, 813
BsiHKCI	CYCGRG	1 cut(s)	140
BsiSI	CCGG	3 cut(s)	593, 833, 956
BslFI	GGGAC	2 cut(s)	31, 547
BslI	CCNNNNNNNGG	3 cut(s)	388, 570, 955
BsmAI	GTCTC	2 cut(s)	310, 386
BsmFI	GGGAC	2 cut(s)	31, 547
BsmI	GAATGC	2 cut(s)	454, 879
BsnI	GGCC	3 cut(s)	239, 836, 860
BsoBI	CYCGRG	1 cut(s)	140
Bsp1286I	GDGCHC	3 cut(s)	361, 762, 813
Bsp143I	GATC	5 cut(s)	30, 83, 363, 601, 918
BspACI	CCGC	2 cut(s)	388, 782
BspANI	GGCC	3 cut(s)	239, 836, 860
BspCNI	CTCAG	2 cut(s)	373, 468
BspFNI	CGCG	1 cut(s)	784
BspLI	GGNNCC	2 cut(s)	32, 279
BspMAI	CTGCAG	1 cut(s)	268
BspMI	ACCTGC	1 cut(s)	265
BspPI	GGATC	3 cut(s)	25, 38, 78
BspQI	GCTCTTC	1 cut(s)	503
BspTI	CTTAAG	1 cut(s)	752
BsrDI	GCAATG	1 cut(s)	222
BsrFI	RCCGGY	2 cut(s)	592, 832
BssAI	RCCGGY	2 cut(s)	592, 832
BssECI	CCNNGG	4 cut(s)	719, 735, 837, 861
BssMI	GATC	5 cut(s)	30, 83, 363, 601, 918
BssNAI	GTATAC	2 cut(s)	408, 590
BssSI	CACGAG	1 cut(s)	914
BssT1I	CCWWGG	2 cut(s)	735, 861
Bst1107I	GTATAC	2 cut(s)	408, 590
Bst2BI	CACGAG	1 cut(s)	914
Bst2UI	CCWGG	1 cut(s)	721
Bst4CI	ACNGT	3 cut(s)	116, 207, 473
Bst6I	CTCTTC	1 cut(s)	503
BstAFI	CTTAAG	1 cut(s)	752
BstC8I	GCNNGC	3 cut(s)	264, 729, 834
BstDEI	CTNAG	3 cut(s)	273, 360, 476
BstDSI	CCRYGG	1 cut(s)	837
BstF5I	GGATG	4 cut(s)	78, 127, 616, 831
BstFNI	CGCG	1 cut(s)	784
BstHHI	GCGC	2 cut(s)	203, 262
BstKTI	GATC	5 cut(s)	33, 86, 366, 604, 921
BstMAI	GTCTC	2 cut(s)	310, 386
BstMBI	GATC	5 cut(s)	30, 83, 363, 601, 918
BstMWI	GCNNNNNNNGC	3 cut(s)	458, 650, 885
BstNI	CCWGG	1 cut(s)	721
BstSCI	CCNGG	1 cut(s)	719
BstSFI	CTRYAG	1 cut(s)	264
BstUI	CGCG	1 cut(s)	784
BstV1I	GCAGC	3 cut(s)	132, 202, 657
BstV2I	GAAGAC	1 cut(s)	141
BstX2I	RGATCY	1 cut(s)	30
BstXI	CCANNNNNNTGG	1 cut(s)	821
BstYI	RGATCY	1 cut(s)	30
BstZ17I	GTATAC	2 cut(s)	408, 590
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	3 cut(s)	239, 836, 860
BtgI	CCRYGG	1 cut(s)	837
BtsCI	GGATG	4 cut(s)	78, 127, 616, 831
BveI	ACCTGC	1 cut(s)	265
Cac8I	GCNNGC	3 cut(s)	264, 729, 834
CaiI	CAGNNNCTG	1 cut(s)	559
CfoI	GCGC	2 cut(s)	203, 262
Cfr10I	RCCGGY	2 cut(s)	592, 832
Cfr13I	GGNCC	3 cut(s)	237, 858, 930
Csp6I	GTAC	1 cut(s)	569
CspAI	ACCGGT	1 cut(s)	592
CviAII	CATG	1 cut(s)	541
CviQI	GTAC	1 cut(s)	569
DdeI	CTNAG	3 cut(s)	273, 360, 476
DpnI	GATC	5 cut(s)	32, 85, 365, 603, 920
DpnII	GATC	5 cut(s)	30, 83, 363, 601, 918
EaeI	YGGCCR	1 cut(s)	834
Eam1104I	CTCTTC	1 cut(s)	503
EarI	CTCTTC	1 cut(s)	503
Ecl136II	GAGCTC	2 cut(s)	359, 811
Eco130I	CCWWGG	2 cut(s)	735, 861
Eco24I	GRGCYC	2 cut(s)	361, 813
Eco32I	GATATC	1 cut(s)	664
Eco47I	GGWCC	1 cut(s)	930
Eco53kI	GAGCTC	2 cut(s)	359, 811
Eco88I	CYCGRG	1 cut(s)	140
EcoICRI	GAGCTC	2 cut(s)	359, 811
EcoO109I	RGGNCCY	1 cut(s)	858
EcoRII	CCWGG	1 cut(s)	719
EcoRV	GATATC	1 cut(s)	664
EcoT14I	CCWWGG	2 cut(s)	735, 861
EcoT38I	GRGCYC	2 cut(s)	361, 813
ErhI	CCWWGG	2 cut(s)	735, 861
FaeI	CATG	1 cut(s)	544
FalI	AAGNNNNNCTT	2 cut(s)	746, 778
FaqI	GGGAC	2 cut(s)	31, 547
FatI	CATG	1 cut(s)	540
FauI	CCCGC	1 cut(s)	381
FblI	GTMKAC	2 cut(s)	407, 589
Fnu4HI	GCNGC	4 cut(s)	121, 191, 671, 782
FokI	GGATG	4 cut(s)	65, 114, 623, 838
FriOI	GRGCYC	2 cut(s)	361, 813
Fsp4HI	GCNGC	4 cut(s)	121, 191, 671, 782
FspBI	CTAG	3 cut(s)	566, 885, 982
GlaI	GCGC	2 cut(s)	202, 261
GluI	GCNGC	4 cut(s)	121, 191, 671, 782
GsuI	CTGGAG	3 cut(s)	264, 270, 797
HaeIII	GGCC	3 cut(s)	239, 836, 860
HapII	CCGG	3 cut(s)	593, 833, 956
HhaI	GCGC	2 cut(s)	203, 262
Hin1II	CATG	1 cut(s)	544
Hin6I	GCGC	2 cut(s)	201, 260
HinP1I	GCGC	2 cut(s)	201, 260
HincII	GTYRAC	1 cut(s)	742
HindII	GTYRAC	1 cut(s)	742
HindIII	AAGCTT	2 cut(s)	651, 935
HinfI	GANTC	3 cut(s)	133, 686, 871
HpaII	CCGG	3 cut(s)	593, 833, 956
HphI	GGTGA	1 cut(s)	671
Hpy166II	GTNNAC	3 cut(s)	408, 590, 742
Hpy188I	TCNGA	6 cut(s)	83, 132, 232, 363, 477, 583
Hpy188III	TCNNGA	4 cut(s)	140, 184, 428, 814
Hpy8I	GTNNAC	3 cut(s)	408, 590, 742
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	2 cut(s)	134, 849
HpyCH4III	ACNGT	3 cut(s)	116, 207, 473
HpyCH4V	TGCA	3 cut(s)	227, 266, 848
HpyF10VI	GCNNNNNNNGC	3 cut(s)	458, 650, 885
HpyF3I	CTNAG	3 cut(s)	273, 360, 476
Hsp92II	CATG	1 cut(s)	544
HspAI	GCGC	2 cut(s)	201, 260
KroI	GCCGGC	1 cut(s)	832
KroNI	GCCGGC	1 cut(s)	834
Kzo9I	GATC	5 cut(s)	30, 83, 363, 601, 918
LguI	GCTCTTC	1 cut(s)	503
LmnI	GCTCC	5 cut(s)	283, 289, 356, 816, 958
Lsp1109I	GCAGC	3 cut(s)	132, 202, 657
LweI	GCATC	3 cut(s)	87, 236, 601
MaeI	CTAG	3 cut(s)	566, 885, 982
MaeIII	GTNAC	3 cut(s)	6, 207, 268
MalI	GATC	5 cut(s)	32, 85, 365, 603, 920
MboI	GATC	5 cut(s)	30, 83, 363, 601, 918
MboII	GAAGA	5 cut(s)	141, 520, 829, 916, 919
MflI	RGATCY	1 cut(s)	30
MhlI	GDGCHC	3 cut(s)	361, 762, 813
MluCI	AATT	3 cut(s)	296, 420, 575
MlyI	GAGTC	2 cut(s)	142, 880
MmeI	TCCRAC	1 cut(s)	334
MnlI	CCTC	9 cut(s)	59, 62, 109, 147, 471, 577, 688, 742, 921
MroNI	GCCGGC	1 cut(s)	832
MroXI	GAANNNNTTC	2 cut(s)	179, 512
MseI	TTAA	4 cut(s)	36, 330, 423, 753
MspA1I	CMGCKG	1 cut(s)	290
MspCI	CTTAAG	1 cut(s)	752
MspI	CCGG	3 cut(s)	593, 833, 956
MspR9I	CCNGG	1 cut(s)	721
Mva1269I	GAATGC	2 cut(s)	454, 879
MvaI	CCWGG	1 cut(s)	721
MvnI	CGCG	1 cut(s)	784
MwoI	GCNNNNNNNGC	3 cut(s)	458, 650, 885
NaeI	GCCGGC	1 cut(s)	834
NdeII	GATC	5 cut(s)	30, 83, 363, 601, 918
NgoMIV	GCCGGC	1 cut(s)	832
NlaIII	CATG	1 cut(s)	544
NlaIV	GGNNCC	2 cut(s)	32, 279
NmuCI	GTSAC	2 cut(s)	207, 268
PaeR7I	CTCGAG	1 cut(s)	140
PciSI	GCTCTTC	1 cut(s)	503
PctI	GAATGC	2 cut(s)	454, 879
PdiI	GCCGGC	1 cut(s)	834
PdmI	GAANNNNTTC	2 cut(s)	179, 512
PfeI	GAWTC	1 cut(s)	686
PinAI	ACCGGT	1 cut(s)	592
PkrI	GCNGC	4 cut(s)	122, 192, 672, 783
PleI	GAGTC	2 cut(s)	141, 879
PpsI	GAGTC	2 cut(s)	141, 879
PshBI	ATTAAT	2 cut(s)	330, 423
PsiI	TTATAA	1 cut(s)	522
Psp124BI	GAGCTC	2 cut(s)	361, 813
Psp6I	CCWGG	1 cut(s)	719
PspGI	CCWGG	1 cut(s)	719
PspN4I	GGNNCC	2 cut(s)	32, 279
PspPI	GGNCC	3 cut(s)	237, 858, 930
PstI	CTGCAG	1 cut(s)	268
PstNI	CAGNNNCTG	1 cut(s)	559
PsuI	RGATCY	1 cut(s)	30
PvuII	CAGCTG	1 cut(s)	290
RsaI	GTAC	1 cut(s)	570
RsaNI	GTAC	1 cut(s)	569
SacI	GAGCTC	2 cut(s)	361, 813
SapI	GCTCTTC	1 cut(s)	503
SaqAI	TTAA	4 cut(s)	36, 330, 423, 753
SatI	GCNGC	4 cut(s)	121, 191, 671, 782
Sau3AI	GATC	5 cut(s)	30, 83, 363, 601, 918
Sau96I	GGNCC	3 cut(s)	237, 858, 930
SchI	GAGTC	2 cut(s)	142, 880
ScrFI	CCNGG	1 cut(s)	721
SduI	GDGCHC	3 cut(s)	361, 762, 813
SfaNI	GCATC	3 cut(s)	87, 236, 601
SfcI	CTRYAG	1 cut(s)	264
Sfr274I	CTCGAG	1 cut(s)	140
SinI	GGWCC	1 cut(s)	930
SlaI	CTCGAG	1 cut(s)	140
SmlI	CTYRAG	3 cut(s)	140, 605, 752
SmoI	CTYRAG	3 cut(s)	140, 605, 752
Sse9I	AATT	3 cut(s)	296, 420, 575
SsiI	CCGC	2 cut(s)	388, 782
SspMI	CTAG	3 cut(s)	566, 885, 982
SstI	GAGCTC	2 cut(s)	361, 813
StyD4I	CCNGG	1 cut(s)	719
StyI	CCWWGG	2 cut(s)	735, 861
TaaI	ACNGT	3 cut(s)	116, 207, 473
TaqI	TCGA	3 cut(s)	141, 152, 441
TasI	AATT	3 cut(s)	296, 420, 575
TauI	GCSGC	1 cut(s)	784
TfiI	GAWTC	1 cut(s)	686
Tru1I	TTAA	4 cut(s)	36, 330, 423, 753
Tru9I	TTAA	4 cut(s)	36, 330, 423, 753
TseFI	GTSAC	2 cut(s)	207, 268
TseI	GCWGC	3 cut(s)	120, 190, 670
Tsp45I	GTSAC	2 cut(s)	207, 268
TspDTI	ATGAA	2 cut(s)	116, 842
TspGWI	ACGGA	4 cut(s)	326, 355, 586, 854
Vha464I	CTTAAG	1 cut(s)	752
VpaK11BI	GGWCC	1 cut(s)	930
VspI	ATTAAT	2 cut(s)	330, 423
XapI	RAATTY	2 cut(s)	296, 575
XhoI	CTCGAG	1 cut(s)	140
XmiI	GTMKAC	2 cut(s)	407, 589
XmnI	GAANNNNTTC	2 cut(s)	179, 512
XspI	CTAG	3 cut(s)	566, 885, 982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.