Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004454

Physical Location & Seq

Genomic Coordinates Forward (+)

27619798 .. 27625215

Gene Structure 5418 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1132100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGACGTCATCGGAGGTTTCGATAAAGGATAATTGCGGCAACCCGAAGGGGGAGAGCTTCTCGTCTGGCTTCAGGGACCACAACGACGCCAGGACCGTCGGCGAGGGGCAGAACGGTCATTCTACTGTTTCCGACGGGAGAGATGCTGAGACGGCGCTTTACACGGAGCTATGGCACGCCTGCGCAGGGCCGCTCGTGACTGTGCCGCGTGAACGGGAACGTGTCTTTTACTTCCCTCAGGGGCACATCGAGCAGGTTGAGGCGTCGACTAATCAGGTGGCTGATCAGCAGATGCCAATGTATAATCTTCCATCGAAGATTCTTTGCCGGGTTATAAATGTATCATTGAAGGCTGAACCAGACACTGATGAAGTATTTGCACAAGTCACTTTACTTCCCGAACCAAATCAAGATGAGAATGCAGTGGAGAAGGAGCCTCCCCCGCCTCCACCACCACGGTTTCAAGTACATTCATTTTGCAAGACCTTAACTGCTTCTGATACAAGCACCCATGGTGGATTTTCAGTGTTAAGGCGGCATGCAGATGAATGCCTTCCACCGCTGGACATGTCTCGCCAACCTCCAACACAGGAGTTGGTTGCCAAAGACTTGCATGGAAGTGAATGGCGCTTTCGCCATATCTTTCGAGGTCAACCACGGAGGCATTTGCTGCAAAGTGGTTGGAGTGTTTTCGTTAGCTCCAAGAGGCTTGTTGCAGGAGATGCATTTATATTTGTAAGGGGTGAGAATGGGGAGCTCCGTGTTGGTGTTAGACGTGCTATGAGACAGCAAGGCAGTGTACCATCTTCCGTGATATCCAGTCACAGTATGCATCTTGGTGTCCTCGCAACTGCATGGCATGCCATTTTAACAGGAACCATGTTCACTGTGTACTACAAGCCAAGGACAAGTCCTGCTGAGTTCATTGTTCCATTTGATCAATACATGGAGTCTGTTAAAAACAACTATTCTATAGGCATGCGGTTTAAGATGAGGTTTGAAGGTGAAGAAGCTCCAGAGCAGAAGTTTACTGGCACCATTATTGGAACAGAAGATGCTGACACCAAAAGGTGGCGAGATTCCAAATGGAGATGCCTTAAGGTGAGATGGGATGAAACCTCTACTATTTCCCGTCCAGAGAGAGTTTCACCTTGGAAAATAGAGCCTGCCCTTGCTCCTCCTGCACTGAATCCACTTCCAATGCCCAGGCCGAAAAGACCTCGAACACACATGGTGCCGTCATCTCCAGATTCCTCTGTCCTCAGTAGGGAAGTTGGATCATCAAAAGTAAATGTTGACCCTGCGATGCCTGGTGGGTTTTCAAGGGTCTTGCAAGGTCAAGAGTTCCCGACCTTGAGAGGCAATTTTGCGGAGAGCCAGTCTGATACTGCTGAGAAATCTGTTGCATGGCCACCGTCGATAGACGAAGAGAAGATTGATGTAGTATCTGCTTCAAGAAGATATGGTTCAGAGAATTGGATGTCCTCAGGGAGGCATGAACCAACTTACACAGATCTGCTGTCAGGCTTTGGGAATAATGTTGATTCCTCACATGGAATCTGTCCCCCTTTTGTCGATCAAGGGGTAGCATCTGCTAATTCAATGCGAAAGCATTCACTGGATCAGGAAGGGAAATATAACTCGCGTTCGTGGTCCATGTTACCATCATCCCTGTCTCTTAGCTTGGACTCTAATCAGAAAGGTCCTCTTGGAAATATGTCGCATCAAGCACAAGGGAATTCTAGATATGGTGGTTTTGGTGACTATTCTGTTGTGAATGGGCATAGAATTGAGCTCCCTCATGGAAATTGGATGATGCCTCTACCACCTCCATCACCTTTTGAGAATCCGGGGAATGCAAGGGACATCATGCCGAGACCACAGATGTTACTAGACAAACGTGAAGCTGTGAACCCTAAAGATGGAAACTGCAAGCTTTTTGGCATTCCCCTGATAACACCAGATCCTGCATTGTTACACAGAAATATGATGAATGAGTCACCTCGTAATAACCAAGCTCATACTTTTGAGTCTGACCTGAATTCTGAAAAATCAAGGGGCTTAAAATCAACAGATAATCTCGTGGCTGTCAGTGAGCCAGCTTTGCAAACTTCTCAGCAACATATCAGAGATGTTCAGGGTAAATCACAGGGTGGTTCAACTAGGAGTTGTACTAAGGTTCACAAGCAGGGTATTGCACTAGGTAGGTCTGTTGATCTTACCAAGTTCAACAACTACAAGGAGCTGATAGCTGAATTGGACATGTTGTTTGAGTTTGGTGGTGAGCTGATGTCTCCGAAAAAGAATTGGATGATCGTTTATACTGATGATGAAGGTGATATGATGCTTGTTGGAGACGATCCTTGGCAGGAATTTTGTGGCATTGTCCGGAAGATTTTCATCTACACCAGAGAGGAAGTCCAAAAGATGAACCCTGGGACTTTGAATGCACAGTGGGAGGATAATCTGTCATCGGGTGCGGAGGGTGTGGATGCAAGAGAGGGGAAGTCTCAACCACGTCCATCTGCATCTACTGCTGAGAAGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

850

Amino Acids

94.62

Weight (kDa)

6.34

Isoelectric Point (pI)

55.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	159 - 260	2.5e-22	B3 DNA binding domain
BRWD_AD	PF25313	288 - 390	1.1e-09	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	308 - 390	4.1e-36	Auxin response factor ancillary domain
AUX_IAA	PF02309	704 - 810	5.5e-11	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62000 AT5G62000 AT5G62000 AT5G62000 AT5G62000
fragaria_vesca	FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310
malus_domestica	MD06G1132100.v1.1 MD14G1148500.v1.1
prunus_persica	Prupe.5G143100_v2.0.a1 Prupe.5G143100_v2.0.a1 Prupe.5G143100_v2.0.a1
pyrus_communis	pycom06g12370 pycom14g12320 pycom14g12330
rosa_chinensis	RchiOBHm_Chr7g0188911
rosa_laevigata	RLG00000004652
rosa_multiflora	Rmu_co8328567.1_g000001 Rmu_ssc0000411.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265950
rosa_rugosa	Rorug06G0494400 Rorug06G0494500
rosa_samantha	Rh7AG099400 Rh7BG101500 Rh7CG102800 Rh7DG101200
rosa_wichuraiana	Rw7G008560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	335
AatII	GACGTC	1 cut(s)	8
Acc16I	TGCGCA	1 cut(s)	184
Acc36I	ACCTGC	1 cut(s)	244
AccB1I	GGYRCC	2 cut(s)	1034, 1234
AccB7I	CCANNNNNTGG	1 cut(s)	1071
AccBSI	CCGCTC	1 cut(s)	193
AccI	GTMKAC	1 cut(s)	266
AccII	CGCG	2 cut(s)	208, 1645
AccIII	TCCGGA	1 cut(s)	2388
AciI	CCGC	9 cut(s)	36, 191, 206, 443, 535, 560, 982, 1370, 2480
AclWI	GGATC	4 cut(s)	1285, 1629, 1958, 2354
AcoI	YGGCCR	1 cut(s)	1409
AcsI	RAATTY	3 cut(s)	1738, 2041, 2372
AcuI	CTGAAG	1 cut(s)	55
AcyI	GRCGYC	3 cut(s)	5, 87, 263
AdeI	CACNNNGTG	2 cut(s)	1234, 2153
AfaI	GTAC	4 cut(s)	468, 801, 893, 2173
AfiI	CCNNNNNNNGG	6 cut(s)	49, 186, 1071, 1267, 1491, 1922
AflII	CTTAAG	1 cut(s)	1097
AflIII	ACRYGT	3 cut(s)	220, 567, 2262
AgsI	TTSAA	9 cut(s)	349, 464, 1001, 1323, 1455, 1602, 2160, 2230, 2446
AjiI	CACGTC	2 cut(s)	776, 2519
AjnI	CCWGG	4 cut(s)	89, 1205, 1309, 2434
Alw21I	GWGCWC	2 cut(s)	759, 1797
Alw26I	GTCTC	8 cut(s)	143, 576, 778, 1680, 1870, 2298, 2349, 2514
AlwI	GGATC	4 cut(s)	1285, 1629, 1958, 2354
AlwNI	CAGNNNCTG	2 cut(s)	365, 1967
Aor13HI	TCCGGA	1 cut(s)	2388
AoxI	GGCC	3 cut(s)	188, 1208, 1409
ApeKI	GCWGC	1 cut(s)	670
ApoI	RAATTY	3 cut(s)	1738, 2041, 2372
ArsI	GACNNNNNNTTYG	2 cut(s)	2252, 2284
Asp700I	GAANNNNTTC	3 cut(s)	552, 1612, 2396
AspLEI	GCGC	3 cut(s)	157, 185, 630
AspS9I	GGNCC	5 cut(s)	76, 93, 188, 1653, 1703
AsuC2I	CCSGG	2 cut(s)	329, 1851
AsuHPI	GGTGA	9 cut(s)	755, 1016, 1114, 1140, 1772, 1827, 1992, 2294, 2348
AvaII	GGWCC	4 cut(s)	76, 93, 1653, 1703
AxyI	CCTNAGG	2 cut(s)	237, 1486
BaeGI	GKGCMC	1 cut(s)	246
BalI	TGGCCA	1 cut(s)	1411
BanI	GGYRCC	2 cut(s)	1034, 1234
BanII	GRGCYC	2 cut(s)	759, 1797
BauI	CACGAG	2 cut(s)	194, 2081
Bbv12I	GWGCWC	2 cut(s)	759, 1797
BbvI	GCAGC	1 cut(s)	657
BccI	CCATC	7 cut(s)	319, 811, 1101, 1672, 1840, 1916, 2530
BceAI	ACGGC	2 cut(s)	168, 1222
BciT130I	CCWGG	4 cut(s)	91, 1207, 1311, 2436
BclI	TGATCA	2 cut(s)	283, 937
BcnI	CCSGG	2 cut(s)	329, 1851
BcoDI	GTCTC	8 cut(s)	143, 576, 778, 1680, 1870, 2298, 2349, 2514
BfaI	CTAG	4 cut(s)	1743, 1892, 2163, 2201
BfmI	CTRYAG	1 cut(s)	972
BfoI	RGCGCY	2 cut(s)	158, 631
BfrI	CTTAAG	1 cut(s)	1097
BfuAI	ACCTGC	1 cut(s)	244
BglII	AGATCT	1 cut(s)	1513
BisI	GCNGC	5 cut(s)	37, 191, 206, 536, 671
BlsI	GCNGC	5 cut(s)	38, 192, 207, 537, 672
Bme1390I	CCNGG	6 cut(s)	91, 329, 1207, 1311, 1851, 2436
Bme18I	GGWCC	4 cut(s)	76, 93, 1653, 1703
BmgBI	CACGTC	2 cut(s)	776, 2519
BmgT120I	GGNCC	5 cut(s)	76, 93, 188, 1653, 1703
BmiI	GGNNCC	5 cut(s)	77, 435, 877, 1036, 1236
BmrFI	CCNGG	6 cut(s)	91, 329, 1207, 1311, 1851, 2436
BplI	GAGNNNNNCTC	2 cut(s)	44, 76
BpmI	CTGGAG	2 cut(s)	999, 1230
BpuEI	CTTGAG	1 cut(s)	1375
BpuMI	CCSGG	2 cut(s)	329, 1851
BsaBI	GATNNNNATC	1 cut(s)	2399
BsaHI	GRCGYC	3 cut(s)	5, 87, 263
BsaI	GGTCTC	1 cut(s)	1870
BsaWI	WCCGGW	1 cut(s)	2388
BsaXI	ACNNNNNCTCC	2 cut(s)	1364, 1394
Bsc4I	CCNNNNNNNGG	6 cut(s)	49, 186, 1071, 1267, 1491, 1922
Bse1I	ACTGG	4 cut(s)	819, 1036, 1378, 1623
Bse21I	CCTNAGG	2 cut(s)	237, 1486
Bse8I	GATNNNNATC	1 cut(s)	2399
BseAI	TCCGGA	1 cut(s)	2388
BseBI	CCWGG	4 cut(s)	91, 1207, 1311, 2436
BseGI	GGATG	6 cut(s)	1117, 1485, 1667, 1818, 2316, 2497
BseJI	GATNNNNATC	1 cut(s)	2399
BseLI	CCNNNNNNNGG	6 cut(s)	49, 186, 1071, 1267, 1491, 1922
BseMII	CTCAG	8 cut(s)	138, 251, 909, 1276, 1383, 1500, 2129, 2529
BseNI	ACTGG	4 cut(s)	819, 1036, 1378, 1623
BseRI	GAGGAG	1 cut(s)	1167
BseSI	GKGCMC	1 cut(s)	246
BseXI	GCAGC	1 cut(s)	657
BsgI	GTGCAG	1 cut(s)	1167
Bsh1236I	CGCG	2 cut(s)	208, 1645
BshFI	GGCC	3 cut(s)	190, 1210, 1411
BshNI	GGYRCC	2 cut(s)	1034, 1234
BsiHKAI	GWGCWC	2 cut(s)	759, 1797
BsiSI	CCGG	3 cut(s)	328, 1850, 2389
BslFI	GGGAC	4 cut(s)	89, 1548, 1877, 2452
BslI	CCNNNNNNNGG	6 cut(s)	49, 186, 1071, 1267, 1491, 1922
BsmAI	GTCTC	8 cut(s)	143, 576, 778, 1680, 1870, 2298, 2349, 2514
BsmBI	CGTCTC	2 cut(s)	143, 2349
BsmFI	GGGAC	4 cut(s)	89, 1548, 1877, 2452
BsmI	GAATGC	6 cut(s)	424, 554, 1612, 1861, 1944, 2452
BsnI	GGCC	3 cut(s)	190, 1210, 1411
Bso31I	GGTCTC	1 cut(s)	1870
Bsp1286I	GDGCHC	3 cut(s)	246, 759, 1797
Bsp13I	TCCGGA	1 cut(s)	2388
Bsp19I	CCATGG	1 cut(s)	511
BspACI	CCGC	9 cut(s)	36, 191, 206, 443, 535, 560, 982, 1370, 2480
BspANI	GGCC	3 cut(s)	190, 1210, 1411
BspCNI	CTCAG	8 cut(s)	139, 250, 910, 1275, 1384, 1499, 2128, 2530
BspEI	TCCGGA	1 cut(s)	2388
BspFNI	CGCG	2 cut(s)	208, 1645
BspLI	GGNNCC	5 cut(s)	77, 435, 877, 1036, 1236
BspMI	ACCTGC	1 cut(s)	244
BspPI	GGATC	4 cut(s)	1285, 1629, 1958, 2354
BspT107I	GGYRCC	2 cut(s)	1034, 1234
BspTI	CTTAAG	1 cut(s)	1097
BspTNI	GGTCTC	1 cut(s)	1870
BsrBI	CCGCTC	1 cut(s)	193
BsrI	ACTGG	4 cut(s)	819, 1036, 1378, 1623
BssNI	GRCGYC	3 cut(s)	5, 87, 263
BssSI	CACGAG	2 cut(s)	194, 2081
BssT1I	CCWWGG	4 cut(s)	511, 902, 1151, 2363
Bst2BI	CACGAG	2 cut(s)	194, 2081
Bst2UI	CCWGG	4 cut(s)	91, 1207, 1311, 2436
Bst4CI	ACNGT	9 cut(s)	97, 116, 127, 202, 459, 827, 889, 1416, 2454
Bst6I	CTCTTC	1 cut(s)	1422
BstACI	GRCGYC	3 cut(s)	5, 87, 263
BstAFI	CTTAAG	1 cut(s)	1097
BstAPI	GCANNNNNTGC	4 cut(s)	670, 722, 860, 2534
BstC8I	GCNNGC	8 cut(s)	177, 181, 540, 861, 980, 1167, 1934, 2100
BstDSI	CCRYGG	3 cut(s)	455, 511, 656
BstENI	CCTNNNNNAGG	1 cut(s)	1489
BstF5I	GGATG	6 cut(s)	1117, 1485, 1667, 1818, 2316, 2497
BstFNI	CGCG	2 cut(s)	208, 1645
BstH2I	RGCGCY	2 cut(s)	158, 631
BstHHI	GCGC	3 cut(s)	157, 185, 630
BstMAI	GTCTC	8 cut(s)	143, 576, 778, 1680, 1870, 2298, 2349, 2514
BstMWI	GCNNNNNNNGC	9 cut(s)	152, 250, 442, 670, 722, 860, 1181, 2104, 2534
BstNI	CCWGG	4 cut(s)	91, 1207, 1311, 2436
BstNSI	RCATGY	5 cut(s)	542, 571, 863, 982, 2266
BstSCI	CCNGG	6 cut(s)	89, 327, 1205, 1309, 1849, 2434
BstSFI	CTRYAG	1 cut(s)	972
BstSLI	GKGCMC	1 cut(s)	246
BstUI	CGCG	2 cut(s)	208, 1645
BstV1I	GCAGC	1 cut(s)	657
BstX2I	RGATCY	2 cut(s)	1513, 1963
BstYI	RGATCY	2 cut(s)	1513, 1963
Bsu36I	CCTNAGG	2 cut(s)	237, 1486
BsuRI	GGCC	3 cut(s)	190, 1210, 1411
BtgI	CCRYGG	3 cut(s)	455, 511, 656
BtgZI	GCGATG	1 cut(s)	1319
BtrI	CACGTC	2 cut(s)	776, 2519
BtsCI	GGATG	6 cut(s)	1117, 1485, 1667, 1818, 2316, 2497
BtsI	GCAGTG	2 cut(s)	429, 802
BtsIMutI	CAGTG	9 cut(s)	363, 429, 531, 802, 885, 1184, 1616, 2098, 2459
BveI	ACCTGC	1 cut(s)	244
Cac8I	GCNNGC	8 cut(s)	177, 181, 540, 861, 980, 1167, 1934, 2100
CaiI	CAGNNNCTG	2 cut(s)	365, 1967
CfoI	GCGC	3 cut(s)	157, 185, 630
Cfr13I	GGNCC	5 cut(s)	76, 93, 188, 1653, 1703
CseI	GACGC	2 cut(s)	95, 252
Csp6I	GTAC	4 cut(s)	467, 800, 892, 2172
CviQI	GTAC	4 cut(s)	467, 800, 892, 2172
DraIII	CACNNNGTG	2 cut(s)	1234, 2153
EaeI	YGGCCR	1 cut(s)	1409
Eam1104I	CTCTTC	1 cut(s)	1422
EarI	CTCTTC	1 cut(s)	1422
Ecl136II	GAGCTC	2 cut(s)	757, 1795
Eco130I	CCWWGG	4 cut(s)	511, 902, 1151, 2363
Eco24I	GRGCYC	2 cut(s)	759, 1797
Eco31I	GGTCTC	1 cut(s)	1870
Eco32I	GATATC	1 cut(s)	816
Eco47I	GGWCC	4 cut(s)	76, 93, 1653, 1703
Eco53kI	GAGCTC	2 cut(s)	757, 1795
Eco57I	CTGAAG	1 cut(s)	55
Eco81I	CCTNAGG	2 cut(s)	237, 1486
EcoICRI	GAGCTC	2 cut(s)	757, 1795
EcoNI	CCTNNNNNAGG	1 cut(s)	1489
EcoO109I	RGGNCCY	1 cut(s)	1703
EcoRI	GAATTC	2 cut(s)	1738, 2041
EcoRII	CCWGG	4 cut(s)	89, 1205, 1309, 2434
EcoRV	GATATC	1 cut(s)	816
EcoT14I	CCWWGG	4 cut(s)	511, 902, 1151, 2363
EcoT22I	ATGCAT	2 cut(s)	727, 834
EcoT38I	GRGCYC	2 cut(s)	759, 1797
ErhI	CCWWGG	4 cut(s)	511, 902, 1151, 2363
Esp3I	CGTCTC	2 cut(s)	143, 2349
FalI	AAGNNNNNCTT	2 cut(s)	1692, 1724
FaqI	GGGAC	4 cut(s)	89, 1548, 1877, 2452
FauI	CCCGC	1 cut(s)	450
FbaI	TGATCA	2 cut(s)	283, 937
FblI	GTMKAC	1 cut(s)	266
Fnu4HI	GCNGC	5 cut(s)	37, 191, 206, 536, 671
FokI	GGATG	6 cut(s)	1124, 1492, 1654, 1825, 2323, 2504
FriOI	GRGCYC	2 cut(s)	759, 1797
Fsp4HI	GCNGC	5 cut(s)	37, 191, 206, 536, 671
FspBI	CTAG	4 cut(s)	1743, 1892, 2163, 2201
FspI	TGCGCA	1 cut(s)	184
GlaI	GCGC	3 cut(s)	156, 184, 629
GluI	GCNGC	5 cut(s)	37, 191, 206, 536, 671
GsuI	CTGGAG	2 cut(s)	999, 1230
HaeII	RGCGCY	2 cut(s)	158, 631
HaeIII	GGCC	3 cut(s)	190, 1210, 1411
HapII	CCGG	3 cut(s)	328, 1850, 2389
HgaI	GACGC	2 cut(s)	95, 252
HhaI	GCGC	3 cut(s)	157, 185, 630
Hin1I	GRCGYC	3 cut(s)	5, 87, 263
Hin6I	GCGC	3 cut(s)	155, 183, 628
HinP1I	GCGC	3 cut(s)	155, 183, 628
HincII	GTYRAC	3 cut(s)	267, 653, 1297
HindII	GTYRAC	3 cut(s)	267, 653, 1297
HindIII	AAGCTT	1 cut(s)	1934
HpaII	CCGG	3 cut(s)	328, 1850, 2389
HphI	GGTGA	9 cut(s)	755, 1016, 1114, 1140, 1772, 1827, 1992, 2294, 2348
Hpy99I	CGWCG	5 cut(s)	89, 101, 137, 268, 1420
HpyAV	CCTTC	7 cut(s)	40, 343, 424, 563, 995, 1622, 2327
HpyCH4III	ACNGT	9 cut(s)	97, 116, 127, 202, 459, 827, 889, 1416, 2454
HpyCH4IV	ACGT	5 cut(s)	5, 220, 775, 1900, 2518
HpyF10VI	GCNNNNNNNGC	9 cut(s)	152, 250, 442, 670, 722, 860, 1181, 2104, 2534
HpySE526I	ACGT	5 cut(s)	5, 220, 775, 1900, 2518
Hsp92I	GRCGYC	3 cut(s)	5, 87, 263
HspAI	GCGC	3 cut(s)	155, 183, 628
Kpn2I	TCCGGA	1 cut(s)	2388
Ksp22I	TGATCA	2 cut(s)	283, 937
LmnI	GCTCC	9 cut(s)	166, 433, 704, 754, 762, 1018, 1180, 1800, 2242
Lsp1109I	GCAGC	1 cut(s)	657
MaeI	CTAG	4 cut(s)	1743, 1892, 2163, 2201
MaeII	ACGT	5 cut(s)	5, 220, 775, 1900, 2518
MaeIII	GTNAC	8 cut(s)	196, 385, 821, 1659, 1760, 1887, 1974, 1998
MbiI	CCGCTC	1 cut(s)	193
MboII	GAAGA	9 cut(s)	299, 328, 798, 1019, 1064, 1439, 1444, 1470, 2404
MflI	RGATCY	2 cut(s)	1513, 1963
MhlI	GDGCHC	3 cut(s)	246, 759, 1797
MlsI	TGGCCA	1 cut(s)	1411
MluNI	TGGCCA	1 cut(s)	1411
MlyI	GAGTC	4 cut(s)	959, 1682, 2006, 2039
MmeI	TCCRAC	5 cut(s)	156, 608, 662, 1255, 2332
Mox20I	TGGCCA	1 cut(s)	1411
Mph1103I	ATGCAT	2 cut(s)	727, 834
MroI	TCCGGA	1 cut(s)	2388
MroXI	GAANNNNTTC	3 cut(s)	552, 1612, 2396
MscI	TGGCCA	1 cut(s)	1411
MseI	TTAA	8 cut(s)	488, 530, 869, 957, 987, 1098, 2063, 2548
MslI	CAYNNNNRTG	3 cut(s)	543, 618, 837
Msp20I	TGGCCA	1 cut(s)	1411
MspA1I	CMGCKG	1 cut(s)	562
MspCI	CTTAAG	1 cut(s)	1097
MspI	CCGG	3 cut(s)	328, 1850, 2389
MspR9I	CCNGG	6 cut(s)	91, 329, 1207, 1311, 1851, 2436
Mva1269I	GAATGC	6 cut(s)	424, 554, 1612, 1861, 1944, 2452
MvaI	CCWGG	4 cut(s)	91, 1207, 1311, 2436
MvnI	CGCG	2 cut(s)	208, 1645
MwoI	GCNNNNNNNGC	9 cut(s)	152, 250, 442, 670, 722, 860, 1181, 2104, 2534
NciI	CCSGG	2 cut(s)	329, 1851
NcoI	CCATGG	1 cut(s)	511
NlaIV	GGNNCC	5 cut(s)	77, 435, 877, 1036, 1236
NmeAIII	GCCGAG	1 cut(s)	1899
NmuCI	GTSAC	5 cut(s)	196, 385, 821, 1760, 1998
NsbI	TGCGCA	1 cut(s)	184
NsiI	ATGCAT	2 cut(s)	727, 834
NspI	RCATGY	5 cut(s)	542, 571, 863, 982, 2266
PaeI	GCATGC	3 cut(s)	542, 863, 982
PasI	CCCWGGG	1 cut(s)	2435
PciI	ACATGT	2 cut(s)	567, 2262
PcsI	WCGNNNNNNNCGW	2 cut(s)	17, 93
PctI	GAATGC	6 cut(s)	424, 554, 1612, 1861, 1944, 2452
PdmI	GAANNNNTTC	3 cut(s)	552, 1612, 2396
PfeI	GAWTC	7 cut(s)	319, 1079, 1189, 1250, 1544, 1557, 1846
PflMI	CCANNNNNTGG	1 cut(s)	1071
PkrI	GCNGC	5 cut(s)	38, 192, 207, 537, 672
PleI	GAGTC	4 cut(s)	958, 1682, 2005, 2038
PpsI	GAGTC	4 cut(s)	958, 1682, 2005, 2038
PpuMI	RGGWCCY	1 cut(s)	1703
PscI	ACATGT	2 cut(s)	567, 2262
PsiI	TTATAA	1 cut(s)	335
Psp124BI	GAGCTC	2 cut(s)	759, 1797
Psp5II	RGGWCCY	1 cut(s)	1703
Psp6I	CCWGG	4 cut(s)	89, 1205, 1309, 2434
PspGI	CCWGG	4 cut(s)	89, 1205, 1309, 2434
PspN4I	GGNNCC	5 cut(s)	77, 435, 877, 1036, 1236
PspPI	GGNCC	5 cut(s)	76, 93, 188, 1653, 1703
PspPPI	RGGWCCY	1 cut(s)	1703
PstNI	CAGNNNCTG	2 cut(s)	365, 1967
PsuI	RGATCY	2 cut(s)	1513, 1963
RsaI	GTAC	4 cut(s)	468, 801, 893, 2173
RsaNI	GTAC	4 cut(s)	467, 800, 892, 2172
RseI	CAYNNNNRTG	3 cut(s)	543, 618, 837
SacI	GAGCTC	2 cut(s)	759, 1797
SalI	GTCGAC	1 cut(s)	265
SaqAI	TTAA	8 cut(s)	488, 530, 869, 957, 987, 1098, 2063, 2548
SatI	GCNGC	5 cut(s)	37, 191, 206, 536, 671
Sau96I	GGNCC	5 cut(s)	76, 93, 188, 1653, 1703
SchI	GAGTC	4 cut(s)	959, 1682, 2006, 2039
ScrFI	CCNGG	6 cut(s)	91, 329, 1207, 1311, 1851, 2436
SduI	GDGCHC	3 cut(s)	246, 759, 1797
SfcI	CTRYAG	1 cut(s)	972
SinI	GGWCC	4 cut(s)	76, 93, 1653, 1703
SmiMI	CAYNNNNRTG	3 cut(s)	543, 618, 837
SmlI	CTYRAG	2 cut(s)	1097, 1354
SmoI	CTYRAG	2 cut(s)	1097, 1354
SphI	GCATGC	3 cut(s)	542, 863, 982
SsiI	CCGC	9 cut(s)	36, 191, 206, 443, 535, 560, 982, 1370, 2480
SspMI	CTAG	4 cut(s)	1743, 1892, 2163, 2201
SstI	GAGCTC	2 cut(s)	759, 1797
StyD4I	CCNGG	6 cut(s)	89, 327, 1205, 1309, 1849, 2434
StyI	CCWWGG	4 cut(s)	511, 902, 1151, 2363
TaaI	ACNGT	9 cut(s)	97, 116, 127, 202, 459, 827, 889, 1416, 2454
TaiI	ACGT	5 cut(s)	8, 223, 778, 1903, 2521
TaqI	TCGA	8 cut(s)	20, 249, 266, 314, 646, 1222, 1418, 1575
TatI	WGTACW	3 cut(s)	466, 891, 2171
TauI	GCSGC	4 cut(s)	39, 193, 208, 538
TfiI	GAWTC	7 cut(s)	319, 1079, 1189, 1250, 1544, 1557, 1846
Tru1I	TTAA	8 cut(s)	488, 530, 869, 957, 987, 1098, 2063, 2548
Tru9I	TTAA	8 cut(s)	488, 530, 869, 957, 987, 1098, 2063, 2548
TscAI	CASTG	9 cut(s)	370, 429, 531, 802, 892, 1191, 1623, 2098, 2459
TseFI	GTSAC	5 cut(s)	196, 385, 821, 1760, 1998
TseI	GCWGC	1 cut(s)	670
Tsp45I	GTSAC	5 cut(s)	196, 385, 821, 1760, 1998
TspGWI	ACGGA	4 cut(s)	179, 673, 749, 799
TspRI	CASTG	9 cut(s)	370, 429, 531, 802, 892, 1191, 1623, 2098, 2459
Van91I	CCANNNNNTGG	1 cut(s)	1071
Vha464I	CTTAAG	1 cut(s)	1097
VpaK11BI	GGWCC	4 cut(s)	76, 93, 1653, 1703
XagI	CCTNNNNNAGG	1 cut(s)	1489
XapI	RAATTY	3 cut(s)	1738, 2041, 2372
XbaI	TCTAGA	1 cut(s)	1742
XceI	RCATGY	5 cut(s)	542, 571, 863, 982, 2266
XmiI	GTMKAC	1 cut(s)	266
XmnI	GAANNNNTTC	3 cut(s)	552, 1612, 2396
XspI	CTAG	4 cut(s)	1743, 1892, 2163, 2201
ZraI	GACGTC	1 cut(s)	6
Zsp2I	ATGCAT	2 cut(s)	727, 834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.