Rorug06G0494500

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004454
Physical Location & Seq
Reverse (-)
61165897 .. 61169691
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0494500.1

Sequence Viewer

Length: 1194 bp
ATGAGCAACCGAGTCATTTCGATGTGGACCGGTTCGACTCGGAACCGAGTCATAGTCATAGTCCTCACCTTCGCCGCCACCGTCAAACTCTCTCACTCTCTCTACCGCAAACTCCGCCGCTCAAGAACTCGCTCCGGAACGATGTCGTTTGCTGCTCCACCGGAAAACGACACCGAATCTCTCAGACGCAGCCGTATTCTCTCCAGCAAGCTCTACCTCGACGTCCCGAGCTCCAAGGTGCCGTTGATTTACTCTTCGACGTATGATATCGCATTTCTCGGCATTGAGAAGCTGCACCCGTTCGATTCGGCTAAATGGGGTCGGATATGTAGATTCCTGTCCATGGATGATGTTGTGGACAAAAATTGGATTGTAGAGCCAAAGGAAGCTACAAAGGAAGATCTTCTTGTGGTGCATACAGAAGCATACTTGAATAGTCTAAAGAAAAGTGCAGATGTTGCTATGATAATTGAGGTACCTCCTGTCGCCCTATTTCCGAATTGCCTTGTGCAGCAGAAAGTTCTCAGTCCATTTCGCAACCAGGTGGGGGGAACTATCTTGGCTGCCAAGCTTGCAAAAGAACGAGGATGGGCCATCAATGTGGGAGGAGGGTTTCATCATTGTTGTGGGGGAAAAGGAGGTGGATTCTGTGCTTATGCTGATATTTCTCTTTGCATACATTTTGCCTTTGTGCGGTTAAACATATCAAGGGTGATGATTATTGATCTTGATGCACATCAAGGAAATGGCCATGAAACGGATTTTGCCAATGACAGTCGAGTCTATATCCTGGATATGTACAACCCTGGAATATATCCATTTGATTATGAAGCAAGGAGATTCATTGATCAGAGGATTGATGTAGCGAGTGGCACTACAACAGCTGAGTACTTGAGAAAGTTAGATGAAGCTCTTGAGGTTGCTGCTCATAATTTTGCTCCTGAGTTGATAGTCTACAATGCTGGAACTGATATTCTAGATGGCGATCCTCTGGGCAGGTTGAAGATCAGTCCTGATGGGATAACTCATAGGGATGAGAAAGTCTTCAGGTTTGCTCTCGAGAGGAATATTCCAATTGTCATGCTCACATCAGGTGGTTACATGAAGTCTAGTGCAAGAGTTATCGCAGACTCGATAGCCAATCTCTCAAACAAATCCCTGATCGATATGGGGAGAAAGACTTCTTCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

397

Amino Acids

44.18

Weight (kDa)

8.88

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hist_deacetyl PF00850 99 - 378 1.4e-56 Histone deacetylase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 779
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 987
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 2 cut(s) 238, 475
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 954
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 5 cut(s) 75, 106, 115, 118, 694
AclWI GGATC 1 cut(s) 980
AcoI YGGCCR 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 1030
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 477, 800, 890
AfiI CCNNNNNNNGG 4 cut(s) 343, 547, 693, 757
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 2 cut(s) 433, 1003
AjnI CCWGG 3 cut(s) 540, 789, 805
AluBI AGCT 7 cut(s) 211, 231, 292, 389, 571, 884, 911
AluI AGCT 7 cut(s) 211, 231, 292, 389, 571, 884, 911
Alw21I GWGCWC 1 cut(s) 233
AlwI GGATC 1 cut(s) 980
Ama87I CYCGRG 2 cut(s) 226, 1058
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 2 cut(s) 591, 748
ApeKI GCWGC 6 cut(s) 152, 189, 292, 511, 563, 923
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 3 cut(s) 402, 1043, 1180
Asp718I GGTACC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 27, 591
AsuHPI GGTGA 2 cut(s) 58, 724
AvaI CYCGRG 2 cut(s) 226, 1058
AvaII GGWCC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 2 cut(s) 238, 475
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 1036
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 6 cut(s) 139, 201, 279, 523, 550, 910
BccI CCATC 4 cut(s) 582, 602, 974, 1010
BceAI ACGGC 2 cut(s) 177, 226
BciT130I CCWGG 3 cut(s) 542, 791, 807
BclI TGATCA 1 cut(s) 847
BfaI CTAG 2 cut(s) 977, 1110
BfuAI ACCTGC 1 cut(s) 987
BglII AGATCT 1 cut(s) 400
BisI GCNGC 8 cut(s) 75, 118, 153, 190, 293, 512, 564, 924
BlsI GCNGC 8 cut(s) 76, 119, 154, 191, 294, 513, 565, 925
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 3 cut(s) 542, 791, 807
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 2 cut(s) 226, 1058
BmgT120I GGNCC 2 cut(s) 27, 591
BmiI GGNNCC 3 cut(s) 44, 240, 477
BmrFI CCNGG 3 cut(s) 542, 791, 807
BmsI GCATC 1 cut(s) 721
BpiI GAAGAC 1 cut(s) 1036
BpmI CTGGAG 1 cut(s) 187
BpuEI CTTGAG 3 cut(s) 106, 913, 935
Bsa29I ATCGAT 1 cut(s) 1164
BsaBI GATNNNNATC 2 cut(s) 735, 984
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 234, 342, 805
BsaWI WCCGGW 3 cut(s) 29, 134, 160
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 547, 693, 757
Bse118I RCCGGY 1 cut(s) 29
Bse8I GATNNNNATC 2 cut(s) 735, 984
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 3 cut(s) 542, 791, 807
BseCI ATCGAT 1 cut(s) 1164
BseDI CCNNGG 3 cut(s) 234, 342, 805
BseGI GGATG 3 cut(s) 352, 593, 1039
BseJI GATNNNNATC 2 cut(s) 735, 984
BseLI CCNNNNNNNGG 4 cut(s) 343, 547, 693, 757
BseMII CTCAG 4 cut(s) 196, 538, 876, 933
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 6 cut(s) 139, 201, 279, 523, 550, 910
BsgI GTGCAG 3 cut(s) 278, 471, 530
BshFI GGCC 2 cut(s) 593, 750
BshNI GGYRCC 2 cut(s) 238, 475
BshTI ACCGGT 1 cut(s) 29
BshVI ATCGAT 1 cut(s) 1164
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 2 cut(s) 226, 1058
BsiSI CCGG 3 cut(s) 30, 135, 161
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 4 cut(s) 343, 547, 693, 757
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 2 cut(s) 593, 750
BsoBI CYCGRG 2 cut(s) 226, 1058
Bsp1286I GDGCHC 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 134
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 5 cut(s) 75, 106, 115, 118, 694
BspANI GGCC 2 cut(s) 593, 750
BspCNI CTCAG 4 cut(s) 195, 537, 877, 934
BspDI ATCGAT 1 cut(s) 1164
BspEI TCCGGA 1 cut(s) 134
BspLI GGNNCC 3 cut(s) 44, 240, 477
BspMI ACCTGC 1 cut(s) 987
BspPI GGATC 1 cut(s) 980
BspT107I GGYRCC 2 cut(s) 238, 475
BsrBI CCGCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 798
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 3 cut(s) 234, 342, 805
BssMI GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 234, 342
Bst2UI CCWGG 3 cut(s) 542, 791, 807
Bst4CI ACNGT 2 cut(s) 82, 776
Bst6I CTCTTC 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 798
BstC8I GCNNGC 2 cut(s) 209, 573
BstDEI CTNAG 4 cut(s) 182, 524, 885, 942
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 3 cut(s) 352, 593, 1039
BstKTI GATC 6 cut(s) 403, 727, 850, 988, 1008, 1164
BstMBI GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 114, 458, 572
BstNI CCWGG 3 cut(s) 542, 791, 807
BstSCI CCNGG 3 cut(s) 540, 789, 805
BstV1I GCAGC 6 cut(s) 139, 201, 279, 523, 550, 910
BstV2I GAAGAC 1 cut(s) 1036
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 1164
BsuRI GGCC 2 cut(s) 593, 750
BsuTUI ATCGAT 1 cut(s) 1164
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 3 cut(s) 352, 593, 1039
BveI ACCTGC 1 cut(s) 987
Cac8I GCNNGC 2 cut(s) 209, 573
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 27, 591
ClaI ATCGAT 1 cut(s) 1164
CseI GACGC 1 cut(s) 195
CsiI ACCWGGT 1 cut(s) 540
Csp6I GTAC 3 cut(s) 476, 799, 889
CspAI ACCGGT 1 cut(s) 29
CviAII CATG 4 cut(s) 343, 752, 1081, 1102
CviQI GTAC 3 cut(s) 476, 799, 889
DdeI CTNAG 4 cut(s) 182, 524, 885, 942
DpnI GATC 6 cut(s) 402, 726, 849, 987, 1007, 1163
DpnII GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
DrdI GACNNNNNNGTC 1 cut(s) 779
DseDI GACNNNNNNGTC 1 cut(s) 779
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 234, 342
Eco24I GRGCYC 1 cut(s) 233
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 1030
Eco88I CYCGRG 2 cut(s) 226, 1058
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 3 cut(s) 540, 789, 805
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 234, 342
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 234, 342
FaeI CATG 4 cut(s) 346, 755, 1084, 1105
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 209
FatI CATG 4 cut(s) 342, 751, 1080, 1101
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 954
Fnu4HI GCNGC 8 cut(s) 75, 118, 153, 190, 293, 512, 564, 924
FokI GGATG 3 cut(s) 359, 600, 1046
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 8 cut(s) 75, 118, 153, 190, 293, 512, 564, 924
FspBI CTAG 2 cut(s) 977, 1110
GluI GCNGC 8 cut(s) 75, 118, 153, 190, 293, 512, 564, 924
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 2 cut(s) 593, 750
HapII CCGG 3 cut(s) 30, 135, 161
HgaI GACGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 346, 755, 1084, 1105
HindIII AAGCTT 1 cut(s) 569
HpaII CCGG 3 cut(s) 30, 135, 161
HphI GGTGA 2 cut(s) 58, 724
Hpy166II GTNNAC 3 cut(s) 27, 358, 955
Hpy188I TCNGA 5 cut(s) 42, 185, 324, 498, 852
Hpy8I GTNNAC 3 cut(s) 27, 358, 955
Hpy99I CGWCG 2 cut(s) 224, 262
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 2 cut(s) 82, 776
HpyCH4IV ACGT 2 cut(s) 222, 260
HpyCH4V TGCA 8 cut(s) 295, 415, 452, 511, 575, 675, 734, 1115
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 458, 572
HpyF3I CTNAG 4 cut(s) 182, 524, 885, 942
HpySE526I ACGT 2 cut(s) 222, 260
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 346, 755, 1084, 1105
Kpn2I TCCGGA 1 cut(s) 134
KpnI GGTACC 1 cut(s) 479
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
LmnI GCTCC 4 cut(s) 137, 160, 236, 943
Lsp1109I GCAGC 6 cut(s) 139, 201, 279, 523, 550, 910
LweI GCATC 1 cut(s) 721
MabI ACCWGGT 1 cut(s) 540
MaeI CTAG 2 cut(s) 977, 1110
MaeII ACGT 2 cut(s) 222, 260
MaeIII GTNAC 1 cut(s) 1097
MalI GATC 6 cut(s) 402, 726, 849, 987, 1007, 1163
MbiI CCGCTC 1 cut(s) 120
MboI GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
MboII GAAGA 6 cut(s) 246, 395, 410, 1015, 1036, 1176
MfeI CAATTG 1 cut(s) 1074
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 5 cut(s) 364, 468, 499, 931, 1074
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 5 cut(s) 21, 31, 57, 789, 1124
MmeI TCCRAC 1 cut(s) 302
Mox20I TGGCCA 1 cut(s) 750
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 3 cut(s) 402, 1043, 1180
MscI TGGCCA 1 cut(s) 750
MseI TTAA 1 cut(s) 698
MslI CAYNNNNRTG 4 cut(s) 20, 599, 624, 1032
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 3 cut(s) 30, 135, 161
MspR9I CCNGG 3 cut(s) 542, 791, 807
MunI CAATTG 1 cut(s) 1074
MvaI CCWGG 3 cut(s) 542, 791, 807
MwoI GCNNNNNNNGC 3 cut(s) 114, 458, 572
NcoI CCATGG 1 cut(s) 342
NdeII GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
NlaIII CATG 4 cut(s) 346, 755, 1084, 1105
NlaIV GGNNCC 3 cut(s) 44, 240, 477
NmeAIII GCCGAG 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 3 cut(s) 402, 1043, 1180
PfeI GAWTC 5 cut(s) 176, 305, 333, 645, 840
PfoI TCCNGGA 1 cut(s) 789
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 8 cut(s) 76, 119, 154, 191, 294, 513, 565, 925
PleI GAGTC 5 cut(s) 20, 31, 56, 788, 1124
PpsI GAGTC 5 cut(s) 20, 31, 56, 788, 1124
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 3 cut(s) 540, 789, 805
PspGI CCWGG 3 cut(s) 540, 789, 805
PspN4I GGNNCC 3 cut(s) 44, 240, 477
PspPI GGNCC 2 cut(s) 27, 591
PsuI RGATCY 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 3 cut(s) 477, 800, 890
RsaNI GTAC 3 cut(s) 476, 799, 889
RseI CAYNNNNRTG 4 cut(s) 20, 599, 624, 1032
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 698
SatI GCNGC 8 cut(s) 75, 118, 153, 190, 293, 512, 564, 924
Sau3AI GATC 6 cut(s) 400, 724, 847, 985, 1005, 1161
Sau96I GGNCC 2 cut(s) 27, 591
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 5 cut(s) 21, 31, 57, 789, 1124
ScrFI CCNGG 3 cut(s) 542, 791, 807
SduI GDGCHC 1 cut(s) 233
SexAI ACCWGGT 1 cut(s) 540
SfaNI GCATC 1 cut(s) 721
Sfr274I CTCGAG 1 cut(s) 1058
SinI GGWCC 1 cut(s) 27
SlaI CTCGAG 1 cut(s) 1058
SmiMI CAYNNNNRTG 4 cut(s) 20, 599, 624, 1032
SmlI CTYRAG 4 cut(s) 121, 892, 914, 1058
SmoI CTYRAG 4 cut(s) 121, 892, 914, 1058
Sse9I AATT 5 cut(s) 364, 468, 499, 931, 1074
SsiI CCGC 5 cut(s) 75, 106, 115, 118, 694
SspI AATATT 1 cut(s) 1069
SspMI CTAG 2 cut(s) 977, 1110
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 540, 789, 805
StyI CCWWGG 2 cut(s) 234, 342
TaaI ACNGT 2 cut(s) 82, 776
TaiI ACGT 2 cut(s) 225, 263
TaqI TCGA 9 cut(s) 20, 35, 219, 257, 303, 778, 1059, 1133, 1164
TasI AATT 5 cut(s) 364, 468, 499, 931, 1074
TatI WGTACW 2 cut(s) 798, 888
TauI GCSGC 2 cut(s) 77, 120
TfiI GAWTC 5 cut(s) 176, 305, 333, 645, 840
Tru1I TTAA 1 cut(s) 698
Tru9I TTAA 1 cut(s) 698
TseI GCWGC 6 cut(s) 152, 189, 292, 511, 563, 923
TspDTI ATGAA 6 cut(s) 605, 768, 832, 843, 921, 1118
TspGWI ACGGA 1 cut(s) 773
VpaK11BI GGWCC 1 cut(s) 27
XbaI TCTAGA 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 1058
XmiI GTMKAC 1 cut(s) 954
XmnI GAANNNNTTC 3 cut(s) 402, 1043, 1180
XspI CTAG 2 cut(s) 977, 1110
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.