pycom06g12370

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004454
Physical Location & Seq
Forward (+)
17501633 .. 17505816
4184 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12370.14

Sequence Viewer

Length: 2535 bp
ATGACGTCATCGGAGGTTTCGATAAAGGATAATTGCGGCAACCCGAGGGGGGAGAGCTTCTCGTCTGGCTTCAGCGACCACAACGACCCCAGGACCGTCGGCGAGGGGCAGAACGGTCATTCTACTGTTTCCGCCGGGAGGGATGCTGAGACGGCGCTATACACGGAGCTATGGCACGCCTGCGCAGGGCCGCTCGTGACTGTGCCGCGTGAACGGGAACGTGTCTTTTACTTCCCTCAGGGGCACATCGAGCAGGTTGAGGCGTCGACTAATCAGGGGGCTGATCAGCAGATGCCAGTGTATAATCTTCCATCGAAGATTCTTTGCCGGGTTATAAATGTATCATTGAAGGCTGAACCAGACACTGATGAAGTATTTGCACAAGTCACTTTACTTCCCGAACCAAATCAAGATGAGAATGCAGTGGAGAAGGAGCCTCCCCCGCCTCTACCACCACGGTTTCAAGTACATTCATTTTGCAAGACCTTAACTGCTTCTGATACAAGCACCCATGGTGGATTTTCAGTGTTAAGGCGGCATGCAGATGAATGCCTTCCACAGCTGGACATGTCTCGCCAACCTCCAACACAGGAGTTGGTTGCCAAAGACTTGCATGGAAGTGAATGGCGCTTTCGCCATATCTTTCGAGGTCAACCACGGAGGCATTTACTGCAAAGTGGTTGGAGTGTTTTCGTTAGCTCCAAGAGGCTTGTTGCAGGAGATGCATTTATATTTTTAAGGGGTGAGAATGGGGAGCTCCGCGTTGGTGTCAGACGTGCTATGAGACAGCAAGGCAGTGTACCATCTTCTGTGATATCCAGTCACAGTATGCATCTTGGTGTCCTCGCAACTGCATGGCATGCCATTTTAACAGGAACCATGTTCACTGTGTACTACAAGCCAAGGACAAGTCCTGCTGAGTTCATTGTTCCATTCGATCAATACATGGAGTCTGTTAAAAACAACTATTCTATAGGCATGCGGTTTAAGATGAGGTTCGAAGGTGAAGAAGCTCCAGAGCAGAAGTTTACTGGCACCATTATTGGAACAGAAGATGCTGACACCAAAAGGTGGCGAGATTCCAAATGGAGATGCCTTAAGGTGAGATGGGATGAAACCTCTACTATTCCCCGTCCAGAGAGAGTTTCACCTTGGAAAATAGAGCCTGCCCTTGCTCCTCCTGCACTGAATCCACTTCCAATGCCCAGGCCGAAAAGACCTCGAACACACATGGTGCCGTCATCTCCAGATTCCTCTGTCCATACTAGGGAAGTAAATGTTGACCCTGCGATGCCTGGTGGGTTTTCAAGGGTCTTGCAAGGTCAAGAGTTCCCGACCTTGAGAGGCAATTTTGCGGAGAGCGAGTCTGATACTACTGAGAAATCTGTTGCATGGCCACCGTCGAAAGACGAAGAGAAGATTGATGTAGTATCTGCTTCAAGAAGATATGGTTCAGAGAACTGGATGTCCTCAGGGAGGCATGAACCAACTTACACAGATCTGCTGTCAGGCTTTGGGAATAATGTTGATTCCTCACATGGTATTTGTCCCCCTTTTGTCGATCAAGGGGTAGCATCTGCTAATTCAATGCGAAAGCATTCACTGGATCAGGAAGGGAAATATAACTCACGTTCGTGGTCCATGTTACCATCATCCCTGTCTCTTAGCTTGGACTCTAATCAGAAAGGTCCTCTTGGAAATATGTCGCATCAAGCACAAGGGAATTCTAGATATGGTGGGTTTGGTGACTATTCTGTTGTGAATGGGCATAGAATTGAGCTCCCTCATGGAAATTGGATGATGCCTCCACCACCTCCATCACCTTTTGAGAATCTGGGGAATGCAAGGGACAACATGCCGAGACCACAGATGTTACTAGACAAACATGAAGCTGTGAAGCCTAAAGATGGAAACTGCAAGCTTTTTGGTATTCCCCTGATAACACCAGATCCTGCATTGTCACACAGAAATATGATGAATGAGTCACCTCGTAATAACCAAGCTCAAACTTTTGAGTCTGACCTGAATTCTGAAAAATCAAGGGGCTCAAAATCAACAGATAATCTCGTGGCTGTCAGTGAGCCAGCTTTGCAAACTTCTCAGCAACATATCAGAGATGTTCAGGGTAAATCACAGGGTGGTTCAACTAGGAGTTGTACTAAGGTTCACAAGCAGGGTATTGCGCTAGGTAGGTCTGTTGATCTTACCAAGTTCAACAACTACAAGGAGCTGATAGCTGAATTGGACATGTTGTTTGAGTTTGGTGGTGAGCTGATGTCTCCGAAAAAGAATTGGATGATCGTTTATACTGATGATGAAGGTGATATGATGCTTGTTGGAGACGATCCCTGGCAGGAATTTTGTGGCATTGTCCGGAAGATTTTCATCTACACCAGAGAGGAAGTCCAAAAGATGAACCCTGGGACCTTGAATGCACATGGGGAGGATAATCTATCATCAGGTGCGGAGGGTGTGGATGCAAGAGAGGGGAAGTCTCAACCACTTCCATCTGCATCTACTGTTGAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

845

Amino Acids

94.04

Weight (kDa)

6.32

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 184
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 2 cut(s) 1034, 1234
AccB7I CCANNNNNTGG 1 cut(s) 1071
AccBSI CCGCTC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 266
AccII CGCG 2 cut(s) 208, 762
AccIII TCCGGA 1 cut(s) 2373
AclWI GGATC 3 cut(s) 1614, 1943, 2339
AcoI YGGCCR 1 cut(s) 1394
AcsI RAATTY 3 cut(s) 1723, 2026, 2357
AcuI CTGAAG 1 cut(s) 55
AcyI GRCGYC 2 cut(s) 5, 263
AdeI CACNNNGTG 2 cut(s) 1234, 2138
AfaI GTAC 4 cut(s) 468, 801, 893, 2158
AfiI CCNNNNNNNGG 7 cut(s) 49, 138, 186, 1071, 1267, 1476, 1907
AflII CTTAAG 1 cut(s) 1097
AflIII ACRYGT 3 cut(s) 220, 567, 2247
AgsI TTSAA 8 cut(s) 349, 464, 1308, 1440, 1587, 2145, 2215, 2431
AjiI CACGTC 1 cut(s) 776
AjnI CCWGG 5 cut(s) 89, 1205, 1294, 2348, 2419
AleI CACNNNNGTG 1 cut(s) 1633
AloI GAACNNNNNNTCC 2 cut(s) 1451, 1483
Alw21I GWGCWC 2 cut(s) 759, 1782
Alw26I GTCTC 8 cut(s) 143, 576, 778, 1665, 1855, 2283, 2334, 2499
AlwI GGATC 3 cut(s) 1614, 1943, 2339
AlwNI CAGNNNCTG 2 cut(s) 365, 1952
Ama87I CYCGRG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 2373
AoxI GGCC 3 cut(s) 188, 1208, 1394
ApoI RAATTY 3 cut(s) 1723, 2026, 2357
ArsI GACNNNNNNTTYG 2 cut(s) 2237, 2269
Asp700I GAANNNNTTC 3 cut(s) 552, 1597, 2381
AspLEI GCGC 4 cut(s) 157, 185, 630, 2185
AspS9I GGNCC 5 cut(s) 93, 188, 1638, 1688, 2424
AsuC2I CCSGG 2 cut(s) 136, 329
AsuHPI GGTGA 9 cut(s) 755, 1016, 1114, 1140, 1757, 1812, 1977, 2279, 2333
AsuII TTCGAA 1 cut(s) 999
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 4 cut(s) 93, 1638, 1688, 2424
AxyI CCTNAGG 2 cut(s) 237, 1471
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 1396
BanI GGYRCC 2 cut(s) 1034, 1234
BanII GRGCYC 3 cut(s) 759, 1782, 2048
BauI CACGAG 2 cut(s) 194, 2066
Bbv12I GWGCWC 2 cut(s) 759, 1782
BccI CCATC 7 cut(s) 319, 811, 1101, 1657, 1825, 1901, 2515
BceAI ACGGC 2 cut(s) 168, 1222
BciT130I CCWGG 5 cut(s) 91, 1207, 1296, 2350, 2421
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 2 cut(s) 136, 329
BcoDI GTCTC 8 cut(s) 143, 576, 778, 1665, 1855, 2283, 2334, 2499
BfaI CTAG 5 cut(s) 1266, 1728, 1877, 2148, 2186
BfmI CTRYAG 1 cut(s) 972
BfoI RGCGCY 2 cut(s) 158, 631
BfrI CTTAAG 1 cut(s) 1097
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 1498
BisI GCNGC 4 cut(s) 37, 191, 206, 536
BlsI GCNGC 4 cut(s) 38, 192, 207, 537
Bme1390I CCNGG 7 cut(s) 91, 136, 329, 1207, 1296, 2350, 2421
Bme18I GGWCC 4 cut(s) 93, 1638, 1688, 2424
BmeT110I CYCGRG 1 cut(s) 43
BmgBI CACGTC 1 cut(s) 776
BmgT120I GGNCC 5 cut(s) 93, 188, 1638, 1688, 2424
BmiI GGNNCC 5 cut(s) 435, 877, 1036, 1236, 2425
BmrFI CCNGG 7 cut(s) 91, 136, 329, 1207, 1296, 2350, 2421
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 2 cut(s) 999, 1230
Bpu14I TTCGAA 1 cut(s) 999
BpuEI CTTGAG 1 cut(s) 1360
BpuMI CCSGG 2 cut(s) 136, 329
BsaBI GATNNNNATC 1 cut(s) 2384
BsaHI GRCGYC 2 cut(s) 5, 263
BsaI GGTCTC 1 cut(s) 1855
BsaWI WCCGGW 1 cut(s) 2373
BsaXI ACNNNNNCTCC 2 cut(s) 1349, 1379
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 138, 186, 1071, 1267, 1476, 1907
Bse1I ACTGG 5 cut(s) 296, 819, 1036, 1466, 1608
Bse21I CCTNAGG 2 cut(s) 237, 1471
Bse8I GATNNNNATC 1 cut(s) 2384
BseAI TCCGGA 1 cut(s) 2373
BseBI CCWGG 5 cut(s) 91, 1207, 1296, 2350, 2421
BseGI GGATG 7 cut(s) 148, 1117, 1470, 1652, 1803, 2301, 2482
BseJI GATNNNNATC 1 cut(s) 2384
BseLI CCNNNNNNNGG 7 cut(s) 49, 138, 186, 1071, 1267, 1476, 1907
BseMII CTCAG 6 cut(s) 138, 251, 909, 1368, 1485, 2114
BseNI ACTGG 5 cut(s) 296, 819, 1036, 1466, 1608
BseRI GAGGAG 1 cut(s) 1167
BseSI GKGCMC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 1167
Bsh1236I CGCG 2 cut(s) 208, 762
BshFI GGCC 3 cut(s) 190, 1210, 1396
BshNI GGYRCC 2 cut(s) 1034, 1234
BsiHKAI GWGCWC 2 cut(s) 759, 1782
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 3 cut(s) 135, 328, 2374
BslFI GGGAC 3 cut(s) 1533, 1862, 2437
BslI CCNNNNNNNGG 7 cut(s) 49, 138, 186, 1071, 1267, 1476, 1907
BsmAI GTCTC 8 cut(s) 143, 576, 778, 1665, 1855, 2283, 2334, 2499
BsmBI CGTCTC 2 cut(s) 143, 2334
BsmFI GGGAC 3 cut(s) 1533, 1862, 2437
BsmI GAATGC 5 cut(s) 424, 554, 1597, 1846, 2437
BsnI GGCC 3 cut(s) 190, 1210, 1396
Bso31I GGTCTC 1 cut(s) 1855
BsoBI CYCGRG 1 cut(s) 43
Bsp119I TTCGAA 1 cut(s) 999
Bsp1286I GDGCHC 4 cut(s) 246, 759, 1782, 2048
Bsp13I TCCGGA 1 cut(s) 2373
Bsp143I GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
Bsp19I CCATGG 1 cut(s) 511
BspANI GGCC 3 cut(s) 190, 1210, 1396
BspCNI CTCAG 6 cut(s) 139, 250, 910, 1369, 1484, 2113
BspEI TCCGGA 1 cut(s) 2373
BspFNI CGCG 2 cut(s) 208, 762
BspLI GGNNCC 5 cut(s) 435, 877, 1036, 1236, 2425
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 3 cut(s) 1614, 1943, 2339
BspT104I TTCGAA 1 cut(s) 999
BspT107I GGYRCC 2 cut(s) 1034, 1234
BspTI CTTAAG 1 cut(s) 1097
BspTNI GGTCTC 1 cut(s) 1855
BsrBI CCGCTC 1 cut(s) 193
BsrI ACTGG 5 cut(s) 296, 819, 1036, 1466, 1608
BssMI GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
BssNI GRCGYC 2 cut(s) 5, 263
BssSI CACGAG 2 cut(s) 194, 2066
BssT1I CCWWGG 3 cut(s) 511, 902, 1151
Bst2BI CACGAG 2 cut(s) 194, 2066
Bst2UI CCWGG 5 cut(s) 91, 1207, 1296, 2350, 2421
Bst4CI ACNGT 9 cut(s) 97, 116, 127, 202, 459, 827, 889, 1401, 2521
Bst6I CTCTTC 1 cut(s) 1407
BstACI GRCGYC 2 cut(s) 5, 263
BstAFI CTTAAG 1 cut(s) 1097
BstAPI GCANNNNNTGC 3 cut(s) 670, 722, 860
BstBI TTCGAA 1 cut(s) 999
BstC8I GCNNGC 8 cut(s) 177, 181, 540, 861, 980, 1167, 1919, 2085
BstDEI CTNAG 8 cut(s) 147, 237, 918, 1377, 1471, 1664, 2100, 2160
BstDSI CCRYGG 3 cut(s) 455, 511, 656
BstENI CCTNNNNNAGG 1 cut(s) 1474
BstF5I GGATG 7 cut(s) 148, 1117, 1470, 1652, 1803, 2301, 2482
BstFNI CGCG 2 cut(s) 208, 762
BstH2I RGCGCY 2 cut(s) 158, 631
BstHHI GCGC 4 cut(s) 157, 185, 630, 2185
BstKTI GATC 9 cut(s) 286, 940, 1501, 1564, 1609, 1951, 2203, 2301, 2347
BstMAI GTCTC 8 cut(s) 143, 576, 778, 1665, 1855, 2283, 2334, 2499
BstMBI GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
BstMWI GCNNNNNNNGC 8 cut(s) 152, 250, 442, 670, 722, 860, 1181, 2089
BstNI CCWGG 5 cut(s) 91, 1207, 1296, 2350, 2421
BstNSI RCATGY 6 cut(s) 542, 571, 863, 982, 1858, 2251
BstSCI CCNGG 7 cut(s) 89, 134, 327, 1205, 1294, 2348, 2419
BstSFI CTRYAG 1 cut(s) 972
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 2 cut(s) 208, 762
BstX2I RGATCY 2 cut(s) 1498, 1948
BstYI RGATCY 2 cut(s) 1498, 1948
Bsu36I CCTNAGG 2 cut(s) 237, 1471
BsuRI GGCC 3 cut(s) 190, 1210, 1396
BtgI CCRYGG 3 cut(s) 455, 511, 656
BtgZI GCGATG 1 cut(s) 1304
BtrI CACGTC 1 cut(s) 776
BtsCI GGATG 7 cut(s) 148, 1117, 1470, 1652, 1803, 2301, 2482
BtsI GCAGTG 2 cut(s) 429, 802
BtsIMutI CAGTG 9 cut(s) 303, 363, 429, 531, 802, 885, 1184, 1601, 2083
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 8 cut(s) 177, 181, 540, 861, 980, 1167, 1919, 2085
CaiI CAGNNNCTG 2 cut(s) 365, 1952
CfoI GCGC 4 cut(s) 157, 185, 630, 2185
Cfr13I GGNCC 5 cut(s) 93, 188, 1638, 1688, 2424
CseI GACGC 1 cut(s) 252
Csp6I GTAC 4 cut(s) 467, 800, 892, 2157
CviQI GTAC 4 cut(s) 467, 800, 892, 2157
DdeI CTNAG 8 cut(s) 147, 237, 918, 1377, 1471, 1664, 2100, 2160
DpnI GATC 9 cut(s) 285, 939, 1500, 1563, 1608, 1950, 2202, 2300, 2346
DpnII GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
DraIII CACNNNGTG 2 cut(s) 1234, 2138
EaeI YGGCCR 1 cut(s) 1394
Eam1104I CTCTTC 1 cut(s) 1407
EarI CTCTTC 1 cut(s) 1407
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 2 cut(s) 757, 1780
Eco130I CCWWGG 3 cut(s) 511, 902, 1151
Eco24I GRGCYC 3 cut(s) 759, 1782, 2048
Eco31I GGTCTC 1 cut(s) 1855
Eco32I GATATC 1 cut(s) 816
Eco47I GGWCC 4 cut(s) 93, 1638, 1688, 2424
Eco53kI GAGCTC 2 cut(s) 757, 1780
Eco57I CTGAAG 1 cut(s) 55
Eco81I CCTNAGG 2 cut(s) 237, 1471
Eco88I CYCGRG 1 cut(s) 43
EcoICRI GAGCTC 2 cut(s) 757, 1780
EcoNI CCTNNNNNAGG 1 cut(s) 1474
EcoO109I RGGNCCY 2 cut(s) 1688, 2424
EcoRI GAATTC 2 cut(s) 1723, 2026
EcoRII CCWGG 5 cut(s) 89, 1205, 1294, 2348, 2419
EcoRV GATATC 1 cut(s) 816
EcoT14I CCWWGG 3 cut(s) 511, 902, 1151
EcoT22I ATGCAT 2 cut(s) 727, 834
EcoT38I GRGCYC 3 cut(s) 759, 1782, 2048
ErhI CCWWGG 3 cut(s) 511, 902, 1151
Esp3I CGTCTC 2 cut(s) 143, 2334
FalI AAGNNNNNCTT 2 cut(s) 1677, 1709
FaqI GGGAC 3 cut(s) 1533, 1862, 2437
FauI CCCGC 1 cut(s) 450
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 4 cut(s) 37, 191, 206, 536
FokI GGATG 7 cut(s) 155, 1124, 1477, 1639, 1810, 2308, 2489
FriOI GRGCYC 3 cut(s) 759, 1782, 2048
Fsp4HI GCNGC 4 cut(s) 37, 191, 206, 536
FspBI CTAG 5 cut(s) 1266, 1728, 1877, 2148, 2186
FspI TGCGCA 1 cut(s) 184
GlaI GCGC 4 cut(s) 156, 184, 629, 2184
GluI GCNGC 4 cut(s) 37, 191, 206, 536
GsuI CTGGAG 2 cut(s) 999, 1230
HaeII RGCGCY 2 cut(s) 158, 631
HaeIII GGCC 3 cut(s) 190, 1210, 1396
HapII CCGG 3 cut(s) 135, 328, 2374
HgaI GACGC 1 cut(s) 252
HhaI GCGC 4 cut(s) 157, 185, 630, 2185
Hin1I GRCGYC 2 cut(s) 5, 263
Hin6I GCGC 4 cut(s) 155, 183, 628, 2183
HinP1I GCGC 4 cut(s) 155, 183, 628, 2183
HincII GTYRAC 3 cut(s) 267, 653, 1282
HindII GTYRAC 3 cut(s) 267, 653, 1282
HindIII AAGCTT 1 cut(s) 1919
HpaII CCGG 3 cut(s) 135, 328, 2374
HphI GGTGA 9 cut(s) 755, 1016, 1114, 1140, 1757, 1812, 1977, 2279, 2333
Hpy166II GTNNAC 9 cut(s) 212, 267, 653, 800, 885, 892, 1029, 1282, 2167
Hpy8I GTNNAC 9 cut(s) 212, 267, 653, 800, 885, 892, 1029, 1282, 2167
Hpy99I CGWCG 3 cut(s) 101, 268, 1405
HpyAV CCTTC 6 cut(s) 343, 424, 563, 995, 1607, 2312
HpyCH4III ACNGT 9 cut(s) 97, 116, 127, 202, 459, 827, 889, 1401, 2521
HpyCH4IV ACGT 4 cut(s) 5, 220, 775, 1630
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 250, 442, 670, 722, 860, 1181, 2089
HpyF3I CTNAG 8 cut(s) 147, 237, 918, 1377, 1471, 1664, 2100, 2160
HpySE526I ACGT 4 cut(s) 5, 220, 775, 1630
Hsp92I GRCGYC 2 cut(s) 5, 263
HspAI GCGC 4 cut(s) 155, 183, 628, 2183
Kpn2I TCCGGA 1 cut(s) 2373
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
LmnI GCTCC 9 cut(s) 166, 433, 704, 754, 762, 1018, 1180, 1785, 2227
MaeI CTAG 5 cut(s) 1266, 1728, 1877, 2148, 2186
MaeII ACGT 4 cut(s) 5, 220, 775, 1630
MaeIII GTNAC 8 cut(s) 196, 385, 821, 1644, 1745, 1872, 1959, 1983
MalI GATC 9 cut(s) 285, 939, 1500, 1563, 1608, 1950, 2202, 2300, 2346
MbiI CCGCTC 1 cut(s) 193
MboI GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
MboII GAAGA 9 cut(s) 299, 328, 798, 1019, 1064, 1424, 1429, 1455, 2389
MflI RGATCY 2 cut(s) 1498, 1948
MhlI GDGCHC 4 cut(s) 246, 759, 1782, 2048
MlsI TGGCCA 1 cut(s) 1396
MluNI TGGCCA 1 cut(s) 1396
MlyI GAGTC 5 cut(s) 959, 1373, 1667, 1991, 2024
MmeI TCCRAC 3 cut(s) 608, 662, 2317
Mox20I TGGCCA 1 cut(s) 1396
Mph1103I ATGCAT 2 cut(s) 727, 834
MroI TCCGGA 1 cut(s) 2373
MroXI GAANNNNTTC 3 cut(s) 552, 1597, 2381
MscI TGGCCA 1 cut(s) 1396
MseI TTAA 8 cut(s) 488, 530, 737, 869, 957, 987, 1098, 2533
MslI CAYNNNNRTG 4 cut(s) 543, 618, 837, 1633
Msp20I TGGCCA 1 cut(s) 1396
MspA1I CMGCKG 1 cut(s) 562
MspCI CTTAAG 1 cut(s) 1097
MspI CCGG 3 cut(s) 135, 328, 2374
MspR9I CCNGG 7 cut(s) 91, 136, 329, 1207, 1296, 2350, 2421
Mva1269I GAATGC 5 cut(s) 424, 554, 1597, 1846, 2437
MvaI CCWGG 5 cut(s) 91, 1207, 1296, 2350, 2421
MvnI CGCG 2 cut(s) 208, 762
MwoI GCNNNNNNNGC 8 cut(s) 152, 250, 442, 670, 722, 860, 1181, 2089
NciI CCSGG 2 cut(s) 136, 329
NcoI CCATGG 1 cut(s) 511
NdeII GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
NlaIV GGNNCC 5 cut(s) 435, 877, 1036, 1236, 2425
NmeAIII GCCGAG 1 cut(s) 1884
NmuCI GTSAC 6 cut(s) 196, 385, 821, 1745, 1959, 1983
NsbI TGCGCA 1 cut(s) 184
NsiI ATGCAT 2 cut(s) 727, 834
NspI RCATGY 6 cut(s) 542, 571, 863, 982, 1858, 2251
NspV TTCGAA 1 cut(s) 999
OliI CACNNNNGTG 1 cut(s) 1633
PaeI GCATGC 3 cut(s) 542, 863, 982
PasI CCCWGGG 1 cut(s) 2420
PciI ACATGT 2 cut(s) 567, 2247
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 5 cut(s) 424, 554, 1597, 1846, 2437
PdmI GAANNNNTTC 3 cut(s) 552, 1597, 2381
PfeI GAWTC 6 cut(s) 319, 1079, 1189, 1250, 1529, 1831
PflMI CCANNNNNTGG 1 cut(s) 1071
PkrI GCNGC 4 cut(s) 38, 192, 207, 537
PleI GAGTC 5 cut(s) 958, 1372, 1667, 1990, 2023
PpsI GAGTC 5 cut(s) 958, 1372, 1667, 1990, 2023
PpuMI RGGWCCY 2 cut(s) 1688, 2424
PscI ACATGT 2 cut(s) 567, 2247
PsiI TTATAA 1 cut(s) 335
Psp124BI GAGCTC 2 cut(s) 759, 1782
Psp5II RGGWCCY 2 cut(s) 1688, 2424
Psp6I CCWGG 5 cut(s) 89, 1205, 1294, 2348, 2419
PspGI CCWGG 5 cut(s) 89, 1205, 1294, 2348, 2419
PspN4I GGNNCC 5 cut(s) 435, 877, 1036, 1236, 2425
PspPI GGNCC 5 cut(s) 93, 188, 1638, 1688, 2424
PspPPI RGGWCCY 2 cut(s) 1688, 2424
PstNI CAGNNNCTG 2 cut(s) 365, 1952
PsuI RGATCY 2 cut(s) 1498, 1948
PvuII CAGCTG 1 cut(s) 562
RsaI GTAC 4 cut(s) 468, 801, 893, 2158
RsaNI GTAC 4 cut(s) 467, 800, 892, 2157
RseI CAYNNNNRTG 4 cut(s) 543, 618, 837, 1633
SacI GAGCTC 2 cut(s) 759, 1782
SalI GTCGAC 1 cut(s) 265
SaqAI TTAA 8 cut(s) 488, 530, 737, 869, 957, 987, 1098, 2533
SatI GCNGC 4 cut(s) 37, 191, 206, 536
Sau3AI GATC 9 cut(s) 283, 937, 1498, 1561, 1606, 1948, 2200, 2298, 2344
Sau96I GGNCC 5 cut(s) 93, 188, 1638, 1688, 2424
SchI GAGTC 5 cut(s) 959, 1373, 1667, 1991, 2024
ScrFI CCNGG 7 cut(s) 91, 136, 329, 1207, 1296, 2350, 2421
SduI GDGCHC 4 cut(s) 246, 759, 1782, 2048
SfcI CTRYAG 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 999
SinI GGWCC 4 cut(s) 93, 1638, 1688, 2424
SmiMI CAYNNNNRTG 4 cut(s) 543, 618, 837, 1633
SmlI CTYRAG 2 cut(s) 1097, 1339
SmoI CTYRAG 2 cut(s) 1097, 1339
SphI GCATGC 3 cut(s) 542, 863, 982
SspMI CTAG 5 cut(s) 1266, 1728, 1877, 2148, 2186
SstI GAGCTC 2 cut(s) 759, 1782
StyD4I CCNGG 7 cut(s) 89, 134, 327, 1205, 1294, 2348, 2419
StyI CCWWGG 3 cut(s) 511, 902, 1151
TaaI ACNGT 9 cut(s) 97, 116, 127, 202, 459, 827, 889, 1401, 2521
TaiI ACGT 4 cut(s) 8, 223, 778, 1633
TatI WGTACW 3 cut(s) 466, 891, 2156
TauI GCSGC 4 cut(s) 39, 193, 208, 538
TfiI GAWTC 6 cut(s) 319, 1079, 1189, 1250, 1529, 1831
Tru1I TTAA 8 cut(s) 488, 530, 737, 869, 957, 987, 1098, 2533
Tru9I TTAA 8 cut(s) 488, 530, 737, 869, 957, 987, 1098, 2533
TscAI CASTG 9 cut(s) 303, 370, 429, 531, 802, 892, 1191, 1608, 2083
TseFI GTSAC 6 cut(s) 196, 385, 821, 1745, 1959, 1983
Tsp45I GTSAC 6 cut(s) 196, 385, 821, 1745, 1959, 1983
TspGWI ACGGA 2 cut(s) 179, 673
TspRI CASTG 9 cut(s) 303, 370, 429, 531, 802, 892, 1191, 1608, 2083
Van91I CCANNNNNTGG 1 cut(s) 1071
Vha464I CTTAAG 1 cut(s) 1097
VpaK11BI GGWCC 4 cut(s) 93, 1638, 1688, 2424
XagI CCTNNNNNAGG 1 cut(s) 1474
XapI RAATTY 3 cut(s) 1723, 2026, 2357
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 6 cut(s) 542, 571, 863, 982, 1858, 2251
XmiI GTMKAC 1 cut(s) 266
XmnI GAANNNNTTC 3 cut(s) 552, 1597, 2381
XspI CTAG 5 cut(s) 1266, 1728, 1877, 2148, 2186
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 2 cut(s) 727, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.