Prupe.5G143100_v2.0.a1

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0004454
Physical Location & Seq
Forward (+)
13246313 .. 13251588
5276 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G143100.2

Sequence Viewer

Length: 2520 bp
ATGACGTCATCGGAGGTTTCGATAAAGGACAATTGCGGGAACCAGAGGGGAGATAGCTTCTCGTCCGGTTTCAGTGACCATAACGATGTTAGGAATAACTTAGAGGGGCAAAACAGTCATCCCACTGTTTCCGCCGCCGGTAGAGATGCTGAGACTGCACTTTACACGGAGCTTTGGCACGCCTGCGCTGGGCCGCTCGTGACTGTGCCGCGTGAACGGGAACGTGTCTTTTACTTCCCTCAGGGACACATCGAGCAGGTGGAGGCGTCAACTAATCAGGTGGCTGACCAACAGATGCCTGTGTATAATCTTCCCTCAAAGATCCTTTGCCGGGTTATCCATGTACAACTGAAGGCTGAACCGGACACTGATGAGGTATTTGCACAAGTCACTTTACTTCCCGAGCCAAGTCAAGATGAGAATACGGTAGAGAAGGAACCTCCTCCCCCTCCACCACCGCGATTTCAAGTACATTCATTTTGTAAGACTCTAACTGCTTCGGATACAAGCACTCATGGTGGATTTTCAGTGCTGAGGCGGCACGCAGATGAATGCCTTCCACAACTGGACATGTCTCGCCAGCCTCCAACCCAGGAGTTGGTTGCAAAAGATTTGCATGCAAATGAGTGGCGCTTTCGCCATATCTTCCGAGGTCAACCCCGGAGGCATTTGCTTCAAAGTGGTTGGAGTGTTTTCGTTAGCTCCAAGAGGCTTGTTGCAGGAGATGCATTTATATTTTTAAGGGGTGAAAATGGGGAGCTCCGTGTTGGTGTTAGACGTGCAATGAGACAGCAAGGCAGTGCCCCATCTTCAGTGATATCTAGTCACAGTATGCACCTAGGTGTCCTTGCTACCGCATGGCACGCCATCTTAACAGGAACGATGTTCACTGTGTACTACAAGCCAAGAACAAGCCCTGCTGAATTCATTGTTCCATTTGACCAGTACATGGAGTCTGTTAAGAACAACTATTCTATAGGTATGAGGTTCAAGATGAGGTTCGAAGGTGAAGAAGCTCCGGAGCAGAGGTTTACTGGTACCATAATTGGAATTGAAGATGCTGACACCAAAAGATGGCGAGATTCCAAATGGAGATCCCTTAAGGTGAGATGGGATGAGACTTCATCCATACCTCGTCCAGACAGAGTTTCACCTTGGAAAATAGAGCCTGCTCTTGCTCCTCCAGCACTGAATCCCCTCCCGATGCCTCGGCCAAAAAGGCCTCGGTCAAACATGGTACCCTCGTCTCCAGACTCTATTCTCACCAGGGAAGGTTCCTCAAAAGTAACTGCAGACCCTGCAATGCCAGGTGGGTTTTCAAGGGTCTTGCAAGGTCAAGAATTCTCGACCTTGAGAGGCAATTTTGTGGATAGCGAGTCTGATACTGCTGAAAAGTCTCTTGCATGGACACCTTCGGTAGATGATGAGAAGATTGACGTGGTTTCTGCTTCAAGAAGACATGGTTCAGAGAACTGGATGCCCTCAGGGAGGCATGAACCGACTTACACAGATCTGTTGTCAGGCTTTGGGACTAATGTCGATTCTTCGCGTGGTATCTGTCCACCTTTTGTTGATCAAGCTGTTGGTAATTCAATGAGAAAGCATTCATTAGATCAGGAAGGCAAGTTTAACTTGCAGTCATGGTCCATGTTGCCCTCATCTCTGTCCCTTAGCTTGGACTCTAATCTTAAGGGTCCTCCCATTGGCAATATGGCTTACCAAGCGCAAGGGAATGCTAGATATGGTGGATTTAGTGACTATTCTGTTCTGAACGGTCATAGAGTTGACCACCCACAAGGAAATTGGTTAATGCCTCCACCCCCATCACATTTCGAGAATCCTGCGAATGCAAGGGAGGCCATGCCACAACATGCGTCACTACAGAAACAGGAGGCTGTGAAGCCTAAAGATGGAAACTACAAGCTCTTTGGTATTCCTCTCATAGCACCAGAGGCTGCATTGTCACACAGAAATGCGATGATTGGGTCACCTCACCATAACCAAGTTCATACTTTTGAGTCTGACCAGAAGTCTGATAAATCAAGGGGCTCAAAATCAGTGGAGAATCCCTTAGCTGTTGGTGAGCCGGATAAACTGTTACAAACTTCTCAGCAACATGTCAGAGATGGTCAGGGTAAACCACAGGGAGGTTCAACTAGGAGTTGTACCAAGGTTCACAAACAAGGTATTGCTCTTGGTAGGTCTGTTGATCTTACGAAGTTCAATAACTACGAGGAACTGATTGCTGCATTGGACCAATTGTTTGAATTTGACGGTGAGCTGATGGCCCCGAAAAAGAATTGGTTGATTGTGTACACTGATGATGAGGGTGATATGATGCTTGTTGGAGATGATCCCTGGCAGGAATTTTGTGGCATAGTCCGGAAGATTTTCATCTACACCAGAGAGGAGGTTCAAAAGATGAACCCTGGGACTCTAAATTCACATGGGGAGGAGAATTTATCATTGGTTGCGGAGGGTGCAGATGCAAGAGAGGGGAAGTCTCAGCTACTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

840

Amino Acids

93.45

Weight (kDa)

6.24

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 247
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 247
Acc65I GGTACC 2 cut(s) 1037, 1237
AccB1I GGYRCC 2 cut(s) 1037, 1237
AccB7I CCANNNNNTGG 3 cut(s) 598, 949, 1074
AccBSI CCGCTC 1 cut(s) 196
AccII CGCG 3 cut(s) 211, 460, 1549
AccIII TCCGGA 2 cut(s) 1018, 2382
AciI CCGC 9 cut(s) 36, 132, 135, 194, 209, 458, 538, 855, 2474
AclWI GGATC 3 cut(s) 316, 1089, 2348
AcoI YGGCCR 1 cut(s) 1211
AcsI RAATTY 6 cut(s) 923, 1340, 2267, 2366, 2440, 2458
AcuI CTGAAG 2 cut(s) 371, 795
AcyI GRCGYC 2 cut(s) 5, 266
AfaI GTAC 8 cut(s) 345, 471, 896, 947, 1039, 1239, 2167, 2315
AflII CTTAAG 2 cut(s) 1100, 1688
AflIII ACRYGT 3 cut(s) 223, 570, 2116
AhdI GACNNNNNGTC 1 cut(s) 2029
AjiI CACGTC 2 cut(s) 779, 1438
AjnI CCWGG 5 cut(s) 591, 1265, 1306, 2357, 2428
AjuI GAANNNNNNNTTGG 2 cut(s) 2450, 2482
AleI CACNNNNGTG 1 cut(s) 840
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 7 cut(s) 146, 579, 781, 1112, 1251, 1401, 2508
AlwI GGATC 3 cut(s) 316, 1089, 2348
AlwNI CAGNNNCTG 2 cut(s) 1298, 1955
Ama87I CYCGRG 1 cut(s) 401
Aor13HI TCCGGA 2 cut(s) 1018, 2382
AoxI GGCC 5 cut(s) 191, 1211, 1220, 1857, 2286
ApeKI GCWGC 2 cut(s) 1955, 2246
ApoI RAATTY 6 cut(s) 923, 1340, 2267, 2366, 2440, 2458
ArsI GACNNNNNNTTYG 2 cut(s) 2246, 2278
Asp700I GAANNNNTTC 3 cut(s) 555, 1603, 2390
Asp718I GGTACC 2 cut(s) 1037, 1237
AspA2I CCTAGG 1 cut(s) 838
AspLEI GCGC 3 cut(s) 188, 633, 1726
AspS9I GGNCC 5 cut(s) 191, 1644, 1694, 2254, 2287
AsuC2I CCSGG 2 cut(s) 332, 661
AsuII TTCGAA 1 cut(s) 1002
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 3 cut(s) 1644, 1694, 2254
AvrII CCTAGG 1 cut(s) 838
AxyI CCTNAGG 2 cut(s) 240, 1483
BaeGI GKGCMC 1 cut(s) 805
BaeI ACNNNNGTAYC 2 cut(s) 1229, 1262
BanI GGYRCC 2 cut(s) 1037, 1237
BanII GRGCYC 2 cut(s) 762, 2051
BauI CACGAG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 1462
Bbv12I GWGCWC 1 cut(s) 762
BbvCI CCTCAGC 1 cut(s) 533
BbvI GCAGC 2 cut(s) 1942, 2233
BccI CCATC 8 cut(s) 814, 875, 1068, 1104, 1831, 1904, 2120, 2278
BcgI CGANNNNNNTGC 2 cut(s) 1823, 1857
BciT130I CCWGG 5 cut(s) 593, 1267, 1308, 2359, 2430
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 1573
BcnI CCSGG 2 cut(s) 332, 661
BcoDI GTCTC 7 cut(s) 146, 579, 781, 1112, 1251, 1401, 2508
BfaI CTAG 4 cut(s) 822, 839, 1737, 2157
BfmI CTRYAG 3 cut(s) 975, 1290, 1880
BfoI RGCGCY 1 cut(s) 634
BfrI CTTAAG 2 cut(s) 1100, 1688
BfuAI ACCTGC 1 cut(s) 247
BfuI GTATCC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 1219
BglII AGATCT 1 cut(s) 1510
BisI GCNGC 6 cut(s) 135, 194, 209, 539, 1956, 2247
BlnI CCTAGG 1 cut(s) 838
BlsI GCNGC 6 cut(s) 136, 195, 210, 540, 1957, 2248
Bme1390I CCNGG 7 cut(s) 332, 593, 661, 1267, 1308, 2359, 2430
Bme18I GGWCC 3 cut(s) 1644, 1694, 2254
BmeRI GACNNNNNGTC 1 cut(s) 2029
BmeT110I CYCGRG 1 cut(s) 401
BmgBI CACGTC 2 cut(s) 779, 1438
BmgT120I GGNCC 5 cut(s) 191, 1644, 1694, 2254, 2287
BmiI GGNNCC 7 cut(s) 41, 438, 1039, 1239, 1276, 1695, 2289
BmrFI CCNGG 7 cut(s) 332, 593, 661, 1267, 1308, 2359, 2430
BmsI GCATC 8 cut(s) 136, 285, 715, 1048, 1194, 1467, 2328, 2476
BoxI GACNNNNGTC 1 cut(s) 1535
BpiI GAAGAC 1 cut(s) 1462
BpmI CTGGAG 2 cut(s) 1167, 1233
Bpu10I CCTNAGC 3 cut(s) 533, 1670, 2071
Bpu14I TTCGAA 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 1372
BpuMI CCSGG 2 cut(s) 332, 661
BsaBI GATNNNNATC 1 cut(s) 2393
BsaHI GRCGYC 2 cut(s) 5, 266
BsaWI WCCGGW 4 cut(s) 65, 361, 1018, 2382
BsaXI ACNNNNNCTCC 2 cut(s) 1013, 1043
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 4 cut(s) 570, 943, 1039, 1478
Bse21I CCTNAGG 2 cut(s) 240, 1483
Bse3DI GCAATG 2 cut(s) 789, 1308
Bse8I GATNNNNATC 1 cut(s) 2393
BseAI TCCGGA 2 cut(s) 1018, 2382
BseBI CCWGG 5 cut(s) 593, 1267, 1308, 2359, 2430
BseGI GGATG 4 cut(s) 118, 1120, 1124, 1482
BseJI GATNNNNATC 1 cut(s) 2393
BseMI GCAATG 2 cut(s) 789, 1308
BseMII CTCAG 6 cut(s) 141, 254, 524, 1497, 2123, 2519
BseNI ACTGG 4 cut(s) 570, 943, 1039, 1478
BseRI GAGGAG 4 cut(s) 432, 1170, 2423, 2468
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 2 cut(s) 1942, 2233
BseYI CCCAGC 1 cut(s) 188
BsgI GTGCAG 2 cut(s) 141, 2502
Bsh1236I CGCG 3 cut(s) 211, 460, 1549
BshFI GGCC 5 cut(s) 193, 1213, 1222, 1859, 2288
BshNI GGYRCC 2 cut(s) 1037, 1237
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 8 cut(s) 66, 138, 331, 362, 661, 1019, 2087, 2383
BslFI GGGAC 4 cut(s) 258, 1543, 1651, 2446
BsmAI GTCTC 7 cut(s) 146, 579, 781, 1112, 1251, 1401, 2508
BsmBI CGTCTC 1 cut(s) 1251
BsmFI GGGAC 4 cut(s) 258, 1543, 1651, 2446
BsmI GAATGC 4 cut(s) 557, 1603, 1738, 1852
BsnI GGCC 5 cut(s) 193, 1213, 1222, 1859, 2288
BsoBI CYCGRG 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 1002
Bsp1286I GDGCHC 3 cut(s) 762, 805, 2051
Bsp13I TCCGGA 2 cut(s) 1018, 2382
Bsp1407I TGTACA 2 cut(s) 343, 2313
Bsp143I GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
BspACI CCGC 9 cut(s) 36, 132, 135, 194, 209, 458, 538, 855, 2474
BspANI GGCC 5 cut(s) 193, 1213, 1222, 1859, 2288
BspCNI CTCAG 6 cut(s) 142, 253, 525, 1496, 2122, 2518
BspEI TCCGGA 2 cut(s) 1018, 2382
BspFNI CGCG 3 cut(s) 211, 460, 1549
BspLI GGNNCC 7 cut(s) 41, 438, 1039, 1239, 1276, 1695, 2289
BspMAI CTGCAG 1 cut(s) 1294
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 3 cut(s) 316, 1089, 2348
BspT104I TTCGAA 1 cut(s) 1002
BspT107I GGYRCC 2 cut(s) 1037, 1237
BspTI CTTAAG 2 cut(s) 1100, 1688
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 2 cut(s) 789, 1308
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 2 cut(s) 343, 2313
BsrI ACTGG 4 cut(s) 570, 943, 1039, 1478
BssAI RCCGGY 1 cut(s) 137
BssMI GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
BssNI GRCGYC 2 cut(s) 5, 266
BssSI CACGAG 1 cut(s) 197
BssT1I CCWWGG 3 cut(s) 838, 1154, 2169
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 5 cut(s) 593, 1267, 1308, 2359, 2430
Bst4CI ACNGT 9 cut(s) 116, 127, 205, 427, 830, 892, 1775, 2097, 2276
BstACI GRCGYC 2 cut(s) 5, 266
BstAFI CTTAAG 2 cut(s) 1100, 1688
BstAPI GCANNNNNTGC 2 cut(s) 725, 1298
BstAUI TGTACA 2 cut(s) 343, 2313
BstBI TTCGAA 1 cut(s) 1002
BstC8I GCNNGC 7 cut(s) 180, 184, 543, 581, 618, 864, 1170
BstDEI CTNAG 9 cut(s) 100, 150, 240, 533, 1483, 1670, 2071, 2109, 2505
BstEII GGTNACC 1 cut(s) 1986
BstENI CCTNNNNNAGG 1 cut(s) 1486
BstF5I GGATG 4 cut(s) 118, 1120, 1124, 1482
BstFNI CGCG 3 cut(s) 211, 460, 1549
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 3 cut(s) 188, 633, 1726
BstKTI GATC 7 cut(s) 324, 1097, 1513, 1576, 1615, 2212, 2356
BstMAI GTCTC 7 cut(s) 146, 579, 781, 1112, 1251, 1401, 2508
BstMBI GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
BstNI CCWGG 5 cut(s) 593, 1267, 1308, 2359, 2430
BstNSI RCATGY 4 cut(s) 574, 620, 1874, 2120
BstPAI GACNNNNGTC 1 cut(s) 1535
BstPI GGTNACC 1 cut(s) 1986
BstSCI CCNGG 7 cut(s) 330, 591, 659, 1265, 1306, 2357, 2428
BstSFI CTRYAG 3 cut(s) 975, 1290, 1880
BstSLI GKGCMC 1 cut(s) 805
BstUI CGCG 3 cut(s) 211, 460, 1549
BstV1I GCAGC 2 cut(s) 1942, 2233
BstV2I GAAGAC 1 cut(s) 1462
BstX2I RGATCY 3 cut(s) 321, 1094, 1510
BstYI RGATCY 3 cut(s) 321, 1094, 1510
Bsu36I CCTNAGG 2 cut(s) 240, 1483
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 5 cut(s) 193, 1213, 1222, 1859, 2288
BtgZI GCGATG 1 cut(s) 1991
BtrI CACGTC 2 cut(s) 779, 1438
BtsCI GGATG 4 cut(s) 118, 1120, 1124, 1482
BtsI GCAGTG 1 cut(s) 805
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 7 cut(s) 180, 184, 543, 581, 618, 864, 1170
CaiI CAGNNNCTG 2 cut(s) 1298, 1955
CfoI GCGC 3 cut(s) 188, 633, 1726
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 5 cut(s) 191, 1644, 1694, 2254, 2287
CseI GACGC 2 cut(s) 255, 1863
Csp6I GTAC 8 cut(s) 344, 470, 895, 946, 1038, 1238, 2166, 2314
CspCI CAANNNNNGTGG 2 cut(s) 2040, 2075
CviQI GTAC 8 cut(s) 344, 470, 895, 946, 1038, 1238, 2166, 2314
DdeI CTNAG 9 cut(s) 100, 150, 240, 533, 1483, 1670, 2071, 2109, 2505
DpnI GATC 7 cut(s) 323, 1096, 1512, 1575, 1614, 2211, 2355
DpnII GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
DriI GACNNNNNGTC 1 cut(s) 2029
EaeI YGGCCR 1 cut(s) 1211
Eam1105I GACNNNNNGTC 1 cut(s) 2029
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 3 cut(s) 838, 1154, 2169
Eco147I AGGCCT 1 cut(s) 1222
Eco24I GRGCYC 2 cut(s) 762, 2051
Eco32I GATATC 1 cut(s) 819
Eco47I GGWCC 3 cut(s) 1644, 1694, 2254
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 371, 795
Eco81I CCTNAGG 2 cut(s) 240, 1483
Eco88I CYCGRG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 1986
EcoICRI GAGCTC 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 1486
EcoO109I RGGNCCY 1 cut(s) 1694
EcoO65I GGTNACC 1 cut(s) 1986
EcoRI GAATTC 2 cut(s) 923, 1340
EcoRII CCWGG 5 cut(s) 591, 1265, 1306, 2357, 2428
EcoRV GATATC 1 cut(s) 819
EcoT14I CCWWGG 3 cut(s) 838, 1154, 2169
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 2 cut(s) 762, 2051
ErhI CCWWGG 3 cut(s) 838, 1154, 2169
Esp3I CGTCTC 1 cut(s) 1251
FalI AAGNNNNNCTT 2 cut(s) 1616, 1648
FaqI GGGAC 4 cut(s) 258, 1543, 1651, 2446
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 1573
Fnu4HI GCNGC 6 cut(s) 135, 194, 209, 539, 1956, 2247
FokI GGATG 4 cut(s) 105, 1111, 1127, 1489
FriOI GRGCYC 2 cut(s) 762, 2051
Fsp4HI GCNGC 6 cut(s) 135, 194, 209, 539, 1956, 2247
FspBI CTAG 4 cut(s) 822, 839, 1737, 2157
GlaI GCGC 3 cut(s) 187, 632, 1725
GluI GCNGC 6 cut(s) 135, 194, 209, 539, 1956, 2247
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 2 cut(s) 1167, 1233
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 5 cut(s) 193, 1213, 1222, 1859, 2288
HapII CCGG 8 cut(s) 66, 138, 331, 362, 661, 1019, 2087, 2383
HgaI GACGC 2 cut(s) 255, 1863
HhaI GCGC 3 cut(s) 188, 633, 1726
Hin1I GRCGYC 2 cut(s) 5, 266
Hin6I GCGC 3 cut(s) 186, 631, 1724
HinP1I GCGC 3 cut(s) 186, 631, 1724
HincII GTYRAC 3 cut(s) 270, 656, 1786
HindII GTYRAC 3 cut(s) 270, 656, 1786
HpaII CCGG 8 cut(s) 66, 138, 331, 362, 661, 1019, 2087, 2383
Hpy188I TCNGA 9 cut(s) 13, 502, 650, 1381, 1468, 1770, 2023, 2035, 2123
HpyAV CCTTC 7 cut(s) 346, 427, 566, 998, 1265, 1422, 1613
HpyCH4III ACNGT 9 cut(s) 116, 127, 205, 427, 830, 892, 1775, 2097, 2276
HpyCH4IV ACGT 4 cut(s) 5, 223, 778, 1437
HpyF3I CTNAG 9 cut(s) 100, 150, 240, 533, 1483, 1670, 2071, 2109, 2505
HpySE526I ACGT 4 cut(s) 5, 223, 778, 1437
Hsp92I GRCGYC 2 cut(s) 5, 266
HspAI GCGC 3 cut(s) 186, 631, 1724
Kpn2I TCCGGA 2 cut(s) 1018, 2382
KpnI GGTACC 2 cut(s) 1041, 1241
Ksp22I TGATCA 1 cut(s) 1573
Kzo9I GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
LmnI GCTCC 7 cut(s) 169, 707, 757, 765, 1021, 1021, 1183
Lsp1109I GCAGC 2 cut(s) 1942, 2233
LweI GCATC 8 cut(s) 136, 285, 715, 1048, 1194, 1467, 2328, 2476
MaeI CTAG 4 cut(s) 822, 839, 1737, 2157
MaeII ACGT 4 cut(s) 5, 223, 778, 1437
MalI GATC 7 cut(s) 323, 1096, 1512, 1575, 1614, 2211, 2355
MbiI CCGCTC 1 cut(s) 196
MboI GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
MboII GAAGA 9 cut(s) 302, 637, 801, 1022, 1067, 1441, 1467, 1536, 2398
MfeI CAATTG 2 cut(s) 31, 2258
MflI RGATCY 3 cut(s) 321, 1094, 1510
MhlI GDGCHC 3 cut(s) 762, 805, 2051
MlyI GAGTC 7 cut(s) 481, 962, 1247, 1385, 1673, 2027, 2428
MmeI TCCRAC 3 cut(s) 611, 665, 2326
Mph1103I ATGCAT 1 cut(s) 730
MroI TCCGGA 2 cut(s) 1018, 2382
MroXI GAANNNNTTC 3 cut(s) 555, 1603, 2390
MseI TTAA 8 cut(s) 740, 872, 960, 1101, 1629, 1689, 1808, 2518
MslI CAYNNNNRTG 5 cut(s) 84, 546, 621, 840, 1971
MspCI CTTAAG 2 cut(s) 1100, 1688
MspI CCGG 8 cut(s) 66, 138, 331, 362, 661, 1019, 2087, 2383
MspR9I CCNGG 7 cut(s) 332, 593, 661, 1267, 1308, 2359, 2430
MunI CAATTG 2 cut(s) 31, 2258
Mva1269I GAATGC 4 cut(s) 557, 1603, 1738, 1852
MvaI CCWGG 5 cut(s) 593, 1267, 1308, 2359, 2430
MvnI CGCG 3 cut(s) 211, 460, 1549
NciI CCSGG 2 cut(s) 332, 661
NdeII GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
NlaIV GGNNCC 7 cut(s) 41, 438, 1039, 1239, 1276, 1695, 2289
NmeAIII GCCGAG 1 cut(s) 1189
NmuCI GTSAC 8 cut(s) 74, 199, 388, 824, 1754, 1875, 1962, 1986
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 4 cut(s) 574, 620, 1874, 2120
NspV TTCGAA 1 cut(s) 1002
OliI CACNNNNGTG 1 cut(s) 840
PaeI GCATGC 1 cut(s) 620
PaqCI CACCTGC 1 cut(s) 247
PasI CCCWGGG 1 cut(s) 2429
PceI AGGCCT 1 cut(s) 1222
PciI ACATGT 2 cut(s) 570, 2116
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 4 cut(s) 557, 1603, 1738, 1852
PdmI GAANNNNTTC 3 cut(s) 555, 1603, 2390
PfeI GAWTC 5 cut(s) 1082, 1192, 1541, 1837, 2065
PflMI CCANNNNNTGG 3 cut(s) 598, 949, 1074
PkrI GCNGC 6 cut(s) 136, 195, 210, 540, 1957, 2248
PleI GAGTC 7 cut(s) 481, 961, 1247, 1384, 1673, 2026, 2428
PpsI GAGTC 7 cut(s) 481, 961, 1247, 1384, 1673, 2026, 2428
PpuMI RGGWCCY 1 cut(s) 1694
PscI ACATGT 2 cut(s) 570, 2116
PshAI GACNNNNGTC 1 cut(s) 1535
Psp124BI GAGCTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 1694
Psp6I CCWGG 5 cut(s) 591, 1265, 1306, 2357, 2428
PspEI GGTNACC 1 cut(s) 1986
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 5 cut(s) 591, 1265, 1306, 2357, 2428
PspN4I GGNNCC 7 cut(s) 41, 438, 1039, 1239, 1276, 1695, 2289
PspPI GGNCC 5 cut(s) 191, 1644, 1694, 2254, 2287
PspPPI RGGWCCY 1 cut(s) 1694
PstI CTGCAG 1 cut(s) 1294
PstNI CAGNNNCTG 2 cut(s) 1298, 1955
PsuI RGATCY 3 cut(s) 321, 1094, 1510
RsaI GTAC 8 cut(s) 345, 471, 896, 947, 1039, 1239, 2167, 2315
RsaNI GTAC 8 cut(s) 344, 470, 895, 946, 1038, 1238, 2166, 2314
RseI CAYNNNNRTG 5 cut(s) 84, 546, 621, 840, 1971
SacI GAGCTC 1 cut(s) 762
SaqAI TTAA 8 cut(s) 740, 872, 960, 1101, 1629, 1689, 1808, 2518
SatI GCNGC 6 cut(s) 135, 194, 209, 539, 1956, 2247
Sau3AI GATC 7 cut(s) 321, 1094, 1510, 1573, 1612, 2209, 2353
Sau96I GGNCC 5 cut(s) 191, 1644, 1694, 2254, 2287
SchI GAGTC 7 cut(s) 481, 962, 1247, 1385, 1673, 2027, 2428
ScrFI CCNGG 7 cut(s) 332, 593, 661, 1267, 1308, 2359, 2430
SduI GDGCHC 3 cut(s) 762, 805, 2051
SfaNI GCATC 8 cut(s) 136, 285, 715, 1048, 1194, 1467, 2328, 2476
SfcI CTRYAG 3 cut(s) 975, 1290, 1880
SfiI GGCCNNNNNGGCC 1 cut(s) 1219
SfuI TTCGAA 1 cut(s) 1002
SinI GGWCC 3 cut(s) 1644, 1694, 2254
SmiMI CAYNNNNRTG 5 cut(s) 84, 546, 621, 840, 1971
SmlI CTYRAG 3 cut(s) 1100, 1351, 1688
SmoI CTYRAG 3 cut(s) 1100, 1351, 1688
SphI GCATGC 1 cut(s) 620
SseBI AGGCCT 1 cut(s) 1222
SsiI CCGC 9 cut(s) 36, 132, 135, 194, 209, 458, 538, 855, 2474
SspMI CTAG 4 cut(s) 822, 839, 1737, 2157
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 1222
StyD4I CCNGG 7 cut(s) 330, 591, 659, 1265, 1306, 2357, 2428
StyI CCWWGG 3 cut(s) 838, 1154, 2169
TaaI ACNGT 9 cut(s) 116, 127, 205, 427, 830, 892, 1775, 2097, 2276
TaiI ACGT 4 cut(s) 8, 226, 781, 1440
TaqI TCGA 6 cut(s) 20, 252, 1002, 1346, 1539, 1833
TaqII GACCGA 1 cut(s) 1215
TatI WGTACW 5 cut(s) 343, 469, 894, 945, 2313
TauI GCSGC 4 cut(s) 137, 196, 211, 541
TfiI GAWTC 5 cut(s) 1082, 1192, 1541, 1837, 2065
Tru1I TTAA 8 cut(s) 740, 872, 960, 1101, 1629, 1689, 1808, 2518
Tru9I TTAA 8 cut(s) 740, 872, 960, 1101, 1629, 1689, 1808, 2518
TseFI GTSAC 8 cut(s) 74, 199, 388, 824, 1754, 1875, 1962, 1986
TseI GCWGC 2 cut(s) 1955, 2246
Tsp45I GTSAC 8 cut(s) 74, 199, 388, 824, 1754, 1875, 1962, 1986
TspDTI ATGAA 9 cut(s) 465, 564, 916, 1113, 1509, 1596, 1997, 2383, 2438
TspGWI ACGGA 2 cut(s) 182, 752
Van91I CCANNNNNTGG 3 cut(s) 598, 949, 1074
Vha464I CTTAAG 2 cut(s) 1100, 1688
VpaK11BI GGWCC 3 cut(s) 1644, 1694, 2254
XagI CCTNNNNNAGG 1 cut(s) 1486
XapI RAATTY 6 cut(s) 923, 1340, 2267, 2366, 2440, 2458
XceI RCATGY 4 cut(s) 574, 620, 1874, 2120
XcmI CCANNNNNNNNNTGG 2 cut(s) 1708, 1800
XmaJI CCTAGG 1 cut(s) 838
XmnI GAANNNNTTC 3 cut(s) 555, 1603, 2390
XspI CTAG 4 cut(s) 822, 839, 1737, 2157
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.