Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004454

Physical Location & Seq

Genomic Coordinates Forward (+)

24121201 .. 24126114

Gene Structure 4914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1148500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGACGTCATCGGAGGTTTCGATAAGGGACAATTGCGGCAATCAGAGGGGGGAGAGCTTCTCGTCCGGCTTTAGCGACCACAACGACGCGAGGAACGCCGGTGAGGGGCATAACGGACATTCTACTGTTTCCGGCGCCGGTAGAGATGCTGAGACGGCGCTTTACACGGAGCTTTGGCACTCCTGCGCAGGGCCGCTCGTGACTGTGCCGCGTGAACGGGAACGTGTCTTTTACTTCCCTCAAGGACACATCGAGCAGGTGGAGGCGTCGACTAATCAGGTGGCTGATCAGCAGATGCCAGTGTATAATCTTCCATCGAAGATTCTCTGCCGGGTTATCAATGTATCATTGAAGGCTGAACCAGACACTGATGAAGTATTTGCACAAGTCACTTTACTTCCCGAATCAAATCAAGATGAGAATGCAGTAGAGAAGGAGCCTCCCCCTCCTCCACCACCACGGTTTCAAGTACATTCATTTTGCAAGACCTTAACTGCTTCCGATACAAGCACCCATGGTGGATTTTCAGTGTTAAGGCGGCATGCAGATGAATGCCTTCCACCACTGGACATGTCTCGCCAACCTCCAACACAGGAGTTGGTTGCCAAAGACTTGCATGGAAATGAATGGCGCTTTCGCCATATCTTCCGAGGTCAACCACGGAGGCATTTGCTGCAAAGTGGTTGGAGTGTTTTCGTTAGCTCCAAGAGGCTTGTTGCAGGCGATGCATTTATATTTTTAAGGGGTGAGAATGGGGAGCTCCGTGTTGGTGTTAGACGTGCTATGAGACAGCAAGGTAGTGTTCCATCTTCTGTGATATCCAGTCACAGTATGCATCTTGGTGTCCTCGCAACCGCATGGCATGCCATCATGACAGGAACCATGTTCACTGTATACTACAAGCCAAGGACAAGCCCCGCTGAGTTCATTGTTCCATTTGAGCAATACATGGAGTCTGTTAAAAACAACTATTCTATAGGCATGAGGTTTAAGATGAGATTTGAAGGTGAAGAAGCTCCAGAGCAGAGGTTTACTGGCACCATAATTGGAATGGAAGATGCTGACACCAAAAGGTGGCGAGATTCCAAATGGAGATGCCTTAAGGTGAGATGGGATGAGACTTCTACTATTCCCCGTCCTGAGAGAGTTTCGCCTTGGAAAATAGAGCCCGCTCTTGCTCCTCCTGCACTGAATCCACTTCCAATGCCCAGGACGAAAAGACCTCGTACAAATATGGTGCCATCATCCCCAGATTCCTCAGTCCTTACTAGGGAAGTTGGTTTGTCAAAAGTAAATGTAGACCCTGCGATGCCTGGTGGGTTTTCAAGAGTCTTGCAAGGTCAAGAATTGCCGACCTTGAGAGCCAGTTTTGCGGAGAGCGAGTCTGATACTGCTGAGAAATCTGTTGCATGGCCACCGTCGATGGATGAAGAGAAGATTGATGGGGTATGTGCTTCAAGAAAATATGGTTCAGAGAACTGGATGTCCTCAGGGAGGCATGAACCAACTTACACAGATTTGCTGTCAGGCTTCGGGAATAATGTTGATTCCTCAAATGGTATCTGTCCACCTTTTGTCGATCAAGGTGTAGTATCTTCTAATTCAATGAGGAAGCATTCACTGGATAAGGAAGGGAAGTATAACTTGCATTCGTGGTCCATGTTACCATCATCTCTGTCACTTAGCTTGGACTCTAATCAGAAAGGACGTCTTGGAAATATGTCATACCAAGCACAAGGGAATTCTAGATATGGTGGGTTTGGTGAATCTTCTGTTATGAATGGGCAGAGAGTTGAGCACCCACATGGAAATTGGATGATGCCTCCACCTCCATCACCTTTTGAGAATCTGGCGAATGCAAGGGAAATCATGCCGAGACCACAGATGTTACTAGACAAACATGAAGCTGTGAAGCCTAAAGATGGAAACTACAAGCTCTTTGGTATTCCCCTGATAACACCAGATCCTGCATTGTCACACAGAAATGCGATGAATGAGTCGCCTCGTAATAACCAAGCTCATACTTTTGAGTCTGACCTGAAGTCTGAAAAATCAAGGGGCTCAAAATCAACAGATAATCCGGTGGCTGTTAGCGAGCCAGCTTTGCAAACTTCTCAGCAACATACCAGAGATGCTCAGGGTAAATCACAGGGTGGTTCAACTAGGAGTTGTACCAAGGTTCACAAGCAGGGTATTGCACTAGGTAGGTCTGTTGATCTTACCAAGTTCAACCACTACGAGGAGCTGATAGCTGAATTGGACATGTTGTTTGAGTTTGGTGGTGAGCTGAGGTCCCCGAAAAAGAATTGGATGATCGTGTATACTGATGATGAAGGTGATATGATGCTTGTTGGAGATGATCCCTGGCAGGAATTTTGTAGCATAGTTCGGAAGATTTTCATCTACACCAGAGAGGAAGTCCAAAAGATGAACCCCGGGACTTTGAATGCACATGGGGAGGATAATCTGTCATTGGGTGCGGAGGGTGTGGATGCAAGAGTGGGGAAGTCTCAACCACTTCCATATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

844

Amino Acids

93.98

Weight (kDa)

6.39

Isoelectric Point (pI)

52.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	160 - 261	1.2e-22	B3 DNA binding domain
BRWD_AD	PF25313	288 - 391	5.4e-10	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	309 - 391	1.2e-36	Auxin response factor ancillary domain
AUX_IAA	PF02309	703 - 810	2.9e-11	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62000 AT5G62000 AT5G62000 AT5G62000 AT5G62000
fragaria_vesca	FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310 FvH4_5g09310
malus_domestica	MD06G1132100.v1.1 MD14G1148500.v1.1
prunus_persica	Prupe.5G143100_v2.0.a1 Prupe.5G143100_v2.0.a1 Prupe.5G143100_v2.0.a1
pyrus_communis	pycom06g12370 pycom14g12320 pycom14g12330
rosa_chinensis	RchiOBHm_Chr7g0188911
rosa_laevigata	RLG00000004652
rosa_multiflora	Rmu_co8328567.1_g000001 Rmu_ssc0000411.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265950
rosa_rugosa	Rorug06G0494400 Rorug06G0494500
rosa_samantha	Rh7AG099400 Rh7BG101500 Rh7CG102800 Rh7DG101200
rosa_wichuraiana	Rw7G008560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	247
AatII	GACGTC	2 cut(s)	8, 1711
Acc16I	TGCGCA	1 cut(s)	187
Acc36I	ACCTGC	1 cut(s)	247
AccB1I	GGYRCC	3 cut(s)	134, 1037, 1237
AccB7I	CCANNNNNTGG	1 cut(s)	1074
AccBSI	CCGCTC	2 cut(s)	196, 1172
AccI	GTMKAC	4 cut(s)	269, 894, 1299, 2321
AccII	CGCG	2 cut(s)	89, 211
AciI	CCGC	9 cut(s)	36, 194, 209, 538, 855, 918, 1170, 1373, 2480
AclWI	GGATC	2 cut(s)	1958, 2354
AcoI	YGGCCR	1 cut(s)	1412
AcsI	RAATTY	2 cut(s)	1741, 2372
AcuI	CTGAAG	1 cut(s)	2060
AcyI	GRCGYC	4 cut(s)	5, 135, 266, 1708
AdeI	CACNNNGTG	1 cut(s)	2153
AfaI	GTAC	3 cut(s)	471, 1228, 2173
AfiI	CCNNNNNNNGG	7 cut(s)	105, 189, 1074, 1270, 1494, 1922, 2239
AflII	CTTAAG	1 cut(s)	1100
AflIII	ACRYGT	3 cut(s)	223, 570, 2262
AgsI	TTSAA	9 cut(s)	352, 467, 1004, 1326, 1458, 1605, 2160, 2230, 2446
AhdI	GACNNNNNGTC	1 cut(s)	2041
AjiI	CACGTC	1 cut(s)	779
AjnI	CCWGG	3 cut(s)	1208, 1312, 2363
AloI	GAACNNNNNNTCC	2 cut(s)	1469, 1501
Alw21I	GWGCWC	2 cut(s)	762, 1800
Alw26I	GTCTC	6 cut(s)	146, 579, 781, 1112, 1870, 2514
AlwI	GGATC	2 cut(s)	1958, 2354
AlwNI	CAGNNNCTG	2 cut(s)	368, 1967
Ama87I	CYCGRG	1 cut(s)	2435
AoxI	GGCC	2 cut(s)	191, 1412
ApeKI	GCWGC	1 cut(s)	673
ApoI	RAATTY	2 cut(s)	1741, 2372
ArsI	GACNNNNNNTTYG	2 cut(s)	2252, 2284
Asp700I	GAANNNNTTC	2 cut(s)	555, 2396
AspLEI	GCGC	4 cut(s)	137, 160, 188, 633
AspS9I	GGNCC	3 cut(s)	191, 1656, 2292
AsuC2I	CCSGG	3 cut(s)	332, 2436, 2437
AsuHPI	GGTGA	8 cut(s)	113, 758, 1019, 1117, 1775, 1827, 2294, 2348
AvaI	CYCGRG	1 cut(s)	2435
AvaII	GGWCC	2 cut(s)	1656, 2292
AxyI	CCTNAGG	1 cut(s)	1489
BalI	TGGCCA	1 cut(s)	1414
BanI	GGYRCC	3 cut(s)	134, 1037, 1237
BanII	GRGCYC	3 cut(s)	762, 1170, 2063
BauI	CACGAG	1 cut(s)	197
Bbv12I	GWGCWC	2 cut(s)	762, 1800
BbvCI	CCTCAGC	1 cut(s)	2288
BbvI	GCAGC	1 cut(s)	660
BceAI	ACGGC	1 cut(s)	171
BciT130I	CCWGG	3 cut(s)	1210, 1314, 2365
BclI	TGATCA	1 cut(s)	286
BcnI	CCSGG	3 cut(s)	332, 2436, 2437
BcoDI	GTCTC	6 cut(s)	146, 579, 781, 1112, 1870, 2514
BfaI	CTAG	5 cut(s)	1269, 1746, 1892, 2163, 2201
BfmI	CTRYAG	1 cut(s)	975
BfoI	RGCGCY	3 cut(s)	138, 161, 634
BfrI	CTTAAG	1 cut(s)	1100
BfuAI	ACCTGC	1 cut(s)	247
BisI	GCNGC	5 cut(s)	37, 194, 209, 539, 674
BlsI	GCNGC	5 cut(s)	38, 195, 210, 540, 675
Bme1390I	CCNGG	6 cut(s)	332, 1210, 1314, 2365, 2436, 2437
Bme18I	GGWCC	2 cut(s)	1656, 2292
BmeRI	GACNNNNNGTC	1 cut(s)	2041
BmeT110I	CYCGRG	1 cut(s)	2435
BmgBI	CACGTC	1 cut(s)	779
BmgT120I	GGNCC	3 cut(s)	191, 1656, 2292
BmiI	GGNNCC	6 cut(s)	136, 438, 880, 1039, 1239, 2294
BmrFI	CCNGG	6 cut(s)	332, 1210, 1314, 2365, 2436, 2437
BplI	GAGNNNNNCTC	2 cut(s)	44, 76
BpmI	CTGGAG	1 cut(s)	1002
Bpu10I	CCTNAGC	2 cut(s)	2136, 2288
BpuEI	CTTGAG	2 cut(s)	225, 1378
BpuMI	CCSGG	3 cut(s)	332, 2436, 2437
BsaBI	GATNNNNATC	1 cut(s)	2399
BsaHI	GRCGYC	4 cut(s)	5, 135, 266, 1708
BsaI	GGTCTC	1 cut(s)	1870
BsaWI	WCCGGW	1 cut(s)	2080
BsaXI	ACNNNNNCTCC	2 cut(s)	1367, 1397
Bsc4I	CCNNNNNNNGG	7 cut(s)	105, 189, 1074, 1270, 1494, 1922, 2239
Bse118I	RCCGGY	2 cut(s)	98, 137
Bse1I	ACTGG	7 cut(s)	299, 570, 822, 1039, 1365, 1484, 1626
Bse21I	CCTNAGG	1 cut(s)	1489
Bse8I	GATNNNNATC	1 cut(s)	2399
BseBI	CCWGG	3 cut(s)	1210, 1314, 2365
BseGI	GGATG	7 cut(s)	1120, 1244, 1432, 1488, 1821, 2316, 2497
BseJI	GATNNNNATC	1 cut(s)	2399
BseLI	CCNNNNNNNGG	7 cut(s)	105, 189, 1074, 1270, 1494, 1922, 2239
BseMII	CTCAG	9 cut(s)	141, 912, 1131, 1272, 1386, 1503, 2129, 2150, 2279
BseNI	ACTGG	7 cut(s)	299, 570, 822, 1039, 1365, 1484, 1626
BseRI	GAGGAG	3 cut(s)	438, 1170, 2255
BseXI	GCAGC	1 cut(s)	660
BsgI	GTGCAG	1 cut(s)	1170
Bsh1236I	CGCG	2 cut(s)	89, 211
BshFI	GGCC	2 cut(s)	193, 1414
BshNI	GGYRCC	3 cut(s)	134, 1037, 1237
BsiHKAI	GWGCWC	2 cut(s)	762, 1800
BsiHKCI	CYCGRG	1 cut(s)	2435
BsiSI	CCGG	7 cut(s)	66, 99, 132, 138, 331, 2081, 2436
BslFI	GGGAC	3 cut(s)	41, 2278, 2452
BslI	CCNNNNNNNGG	7 cut(s)	105, 189, 1074, 1270, 1494, 1922, 2239
BsmAI	GTCTC	6 cut(s)	146, 579, 781, 1112, 1870, 2514
BsmBI	CGTCTC	1 cut(s)	146
BsmFI	GGGAC	3 cut(s)	41, 2278, 2452
BsmI	GAATGC	6 cut(s)	427, 557, 1615, 1648, 1861, 2452
BsnI	GGCC	2 cut(s)	193, 1414
Bso31I	GGTCTC	1 cut(s)	1870
BsoBI	CYCGRG	1 cut(s)	2435
Bsp1286I	GDGCHC	4 cut(s)	762, 1170, 1800, 2063
Bsp143I	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
Bsp19I	CCATGG	1 cut(s)	514
BspACI	CCGC	9 cut(s)	36, 194, 209, 538, 855, 918, 1170, 1373, 2480
BspANI	GGCC	2 cut(s)	193, 1414
BspCNI	CTCAG	9 cut(s)	142, 913, 1132, 1271, 1387, 1502, 2128, 2149, 2280
BspFNI	CGCG	2 cut(s)	89, 211
BspHI	TCATGA	1 cut(s)	870
BspLI	GGNNCC	6 cut(s)	136, 438, 880, 1039, 1239, 2294
BspMI	ACCTGC	1 cut(s)	247
BspPI	GGATC	2 cut(s)	1958, 2354
BspT107I	GGYRCC	3 cut(s)	134, 1037, 1237
BspTI	CTTAAG	1 cut(s)	1100
BspTNI	GGTCTC	1 cut(s)	1870
BsrBI	CCGCTC	2 cut(s)	196, 1172
BsrFI	RCCGGY	2 cut(s)	98, 137
BsrI	ACTGG	7 cut(s)	299, 570, 822, 1039, 1365, 1484, 1626
BssAI	RCCGGY	2 cut(s)	98, 137
BssMI	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
BssNAI	GTATAC	2 cut(s)	895, 2322
BssNI	GRCGYC	4 cut(s)	5, 135, 266, 1708
BssSI	CACGAG	1 cut(s)	197
BssT1I	CCWWGG	4 cut(s)	514, 905, 1154, 2175
Bst1107I	GTATAC	2 cut(s)	895, 2322
Bst2BI	CACGAG	1 cut(s)	197
Bst2UI	CCWGG	3 cut(s)	1210, 1314, 2365
Bst4CI	ACNGT	6 cut(s)	127, 205, 462, 830, 892, 1419
Bst6I	CTCTTC	1 cut(s)	1425
BstACI	GRCGYC	4 cut(s)	5, 135, 266, 1708
BstAFI	CTTAAG	1 cut(s)	1100
BstAPI	GCANNNNNTGC	3 cut(s)	673, 725, 863
BstC8I	GCNNGC	6 cut(s)	543, 721, 864, 1170, 2096, 2100
BstDSI	CCRYGG	3 cut(s)	458, 514, 659
BstENI	CCTNNNNNAGG	1 cut(s)	1492
BstF5I	GGATG	7 cut(s)	1120, 1244, 1432, 1488, 1821, 2316, 2497
BstFNI	CGCG	2 cut(s)	89, 211
BstH2I	RGCGCY	3 cut(s)	138, 161, 634
BstHHI	GCGC	4 cut(s)	137, 160, 188, 633
BstKTI	GATC	6 cut(s)	289, 1582, 1966, 2218, 2316, 2362
BstMAI	GTCTC	6 cut(s)	146, 579, 781, 1112, 1870, 2514
BstMBI	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
BstMWI	GCNNNNNNNGC	8 cut(s)	95, 155, 673, 725, 863, 1184, 1370, 2104
BstNI	CCWGG	3 cut(s)	1210, 1314, 2365
BstNSI	RCATGY	4 cut(s)	545, 574, 866, 2266
BstSCI	CCNGG	6 cut(s)	330, 1208, 1312, 2363, 2434, 2435
BstSFI	CTRYAG	1 cut(s)	975
BstUI	CGCG	2 cut(s)	89, 211
BstV1I	GCAGC	1 cut(s)	660
BstX2I	RGATCY	1 cut(s)	1963
BstYI	RGATCY	1 cut(s)	1963
BstZ17I	GTATAC	2 cut(s)	895, 2322
Bsu36I	CCTNAGG	1 cut(s)	1489
BsuRI	GGCC	2 cut(s)	193, 1414
BtgI	CCRYGG	3 cut(s)	458, 514, 659
BtgZI	GCGATG	3 cut(s)	738, 1322, 2003
BtrI	CACGTC	1 cut(s)	779
BtsCI	GGATG	7 cut(s)	1120, 1244, 1432, 1488, 1821, 2316, 2497
BtsIMutI	CAGTG	7 cut(s)	306, 366, 534, 563, 888, 1187, 1619
BveI	ACCTGC	1 cut(s)	247
Cac8I	GCNNGC	6 cut(s)	543, 721, 864, 1170, 2096, 2100
CaiI	CAGNNNCTG	2 cut(s)	368, 1967
CciI	TCATGA	1 cut(s)	870
CfoI	GCGC	4 cut(s)	137, 160, 188, 633
Cfr10I	RCCGGY	2 cut(s)	98, 137
Cfr13I	GGNCC	3 cut(s)	191, 1656, 2292
Cfr9I	CCCGGG	1 cut(s)	2435
CseI	GACGC	2 cut(s)	95, 255
Csp6I	GTAC	3 cut(s)	470, 1227, 2172
CviQI	GTAC	3 cut(s)	470, 1227, 2172
DinI	GGCGCC	1 cut(s)	136
DpnI	GATC	6 cut(s)	288, 1581, 1965, 2217, 2315, 2361
DpnII	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
DraIII	CACNNNGTG	1 cut(s)	2153
DriI	GACNNNNNGTC	1 cut(s)	2041
EaeI	YGGCCR	1 cut(s)	1412
Eam1104I	CTCTTC	1 cut(s)	1425
Eam1105I	GACNNNNNGTC	1 cut(s)	2041
EarI	CTCTTC	1 cut(s)	1425
Ecl136II	GAGCTC	1 cut(s)	760
Eco130I	CCWWGG	4 cut(s)	514, 905, 1154, 2175
Eco24I	GRGCYC	3 cut(s)	762, 1170, 2063
Eco31I	GGTCTC	1 cut(s)	1870
Eco32I	GATATC	1 cut(s)	819
Eco47I	GGWCC	2 cut(s)	1656, 2292
Eco53kI	GAGCTC	1 cut(s)	760
Eco57I	CTGAAG	1 cut(s)	2060
Eco81I	CCTNAGG	1 cut(s)	1489
Eco88I	CYCGRG	1 cut(s)	2435
EcoICRI	GAGCTC	1 cut(s)	760
EcoNI	CCTNNNNNAGG	1 cut(s)	1492
EcoO109I	RGGNCCY	1 cut(s)	2292
EcoRI	GAATTC	1 cut(s)	1741
EcoRII	CCWGG	3 cut(s)	1208, 1312, 2363
EcoRV	GATATC	1 cut(s)	819
EcoT14I	CCWWGG	4 cut(s)	514, 905, 1154, 2175
EcoT22I	ATGCAT	3 cut(s)	730, 837, 2530
EcoT38I	GRGCYC	3 cut(s)	762, 1170, 2063
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
ErhI	CCWWGG	4 cut(s)	514, 905, 1154, 2175
Esp3I	CGTCTC	1 cut(s)	146
FalI	AAGNNNNNCTT	4 cut(s)	1628, 1660, 1695, 1727
FaqI	GGGAC	3 cut(s)	41, 2278, 2452
FauI	CCCGC	2 cut(s)	925, 1177
FauNDI	CATATG	1 cut(s)	2524
FbaI	TGATCA	1 cut(s)	286
FblI	GTMKAC	4 cut(s)	269, 894, 1299, 2321
Fnu4HI	GCNGC	5 cut(s)	37, 194, 209, 539, 674
FokI	GGATG	7 cut(s)	1127, 1231, 1439, 1495, 1828, 2323, 2504
FriOI	GRGCYC	3 cut(s)	762, 1170, 2063
Fsp4HI	GCNGC	5 cut(s)	37, 194, 209, 539, 674
FspBI	CTAG	5 cut(s)	1269, 1746, 1892, 2163, 2201
FspI	TGCGCA	1 cut(s)	187
GlaI	GCGC	4 cut(s)	136, 159, 187, 632
GluI	GCNGC	5 cut(s)	37, 194, 209, 539, 674
GsuI	CTGGAG	1 cut(s)	1002
HaeII	RGCGCY	3 cut(s)	138, 161, 634
HaeIII	GGCC	2 cut(s)	193, 1414
HapII	CCGG	7 cut(s)	66, 99, 132, 138, 331, 2081, 2436
HgaI	GACGC	2 cut(s)	95, 255
HhaI	GCGC	4 cut(s)	137, 160, 188, 633
Hin1I	GRCGYC	4 cut(s)	5, 135, 266, 1708
Hin6I	GCGC	4 cut(s)	135, 158, 186, 631
HinP1I	GCGC	4 cut(s)	135, 158, 186, 631
HincII	GTYRAC	2 cut(s)	270, 656
HindII	GTYRAC	2 cut(s)	270, 656
HpaII	CCGG	7 cut(s)	66, 99, 132, 138, 331, 2081, 2436
HphI	GGTGA	8 cut(s)	113, 758, 1019, 1117, 1775, 1827, 2294, 2348
Hpy99I	CGWCG	3 cut(s)	89, 271, 1423
HpyAV	CCTTC	6 cut(s)	346, 427, 566, 998, 1625, 2327
HpyCH4III	ACNGT	6 cut(s)	127, 205, 462, 830, 892, 1419
HpyCH4IV	ACGT	4 cut(s)	5, 223, 778, 1708
HpyF10VI	GCNNNNNNNGC	8 cut(s)	95, 155, 673, 725, 863, 1184, 1370, 2104
HpySE526I	ACGT	4 cut(s)	5, 223, 778, 1708
Hsp92I	GRCGYC	4 cut(s)	5, 135, 266, 1708
HspAI	GCGC	4 cut(s)	135, 158, 186, 631
KasI	GGCGCC	1 cut(s)	134
Ksp22I	TGATCA	1 cut(s)	286
Kzo9I	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
LmnI	GCTCC	8 cut(s)	169, 436, 707, 757, 765, 1021, 1183, 2242
Lsp1109I	GCAGC	1 cut(s)	660
MaeI	CTAG	5 cut(s)	1269, 1746, 1892, 2163, 2201
MaeII	ACGT	4 cut(s)	5, 223, 778, 1708
MaeIII	GTNAC	7 cut(s)	199, 388, 824, 1662, 1677, 1887, 1974
MalI	GATC	6 cut(s)	288, 1581, 1965, 2217, 2315, 2361
MbiI	CCGCTC	2 cut(s)	196, 1172
MboI	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
MfeI	CAATTG	1 cut(s)	31
MflI	RGATCY	1 cut(s)	1963
MhlI	GDGCHC	4 cut(s)	762, 1170, 1800, 2063
MlsI	TGGCCA	1 cut(s)	1414
MluCI	AATT	9 cut(s)	31, 1044, 1346, 1600, 1741, 1810, 2255, 2305, 2372
MluNI	TGGCCA	1 cut(s)	1414
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	6 cut(s)	962, 1338, 1391, 1685, 2006, 2039
MmeI	TCCRAC	3 cut(s)	611, 665, 2332
Mox20I	TGGCCA	1 cut(s)	1414
Mph1103I	ATGCAT	3 cut(s)	730, 837, 2530
MroXI	GAANNNNTTC	2 cut(s)	555, 2396
MscI	TGGCCA	1 cut(s)	1414
MseI	TTAA	6 cut(s)	491, 533, 740, 960, 990, 1101
MslI	CAYNNNNRTG	5 cut(s)	546, 621, 840, 1803, 1983
Msp20I	TGGCCA	1 cut(s)	1414
MspA1I	CMGCKG	1 cut(s)	920
MspCI	CTTAAG	1 cut(s)	1100
MspI	CCGG	7 cut(s)	66, 99, 132, 138, 331, 2081, 2436
MspR9I	CCNGG	6 cut(s)	332, 1210, 1314, 2365, 2436, 2437
MunI	CAATTG	1 cut(s)	31
Mva1269I	GAATGC	6 cut(s)	427, 557, 1615, 1648, 1861, 2452
MvaI	CCWGG	3 cut(s)	1210, 1314, 2365
MvnI	CGCG	2 cut(s)	89, 211
MwoI	GCNNNNNNNGC	8 cut(s)	95, 155, 673, 725, 863, 1184, 1370, 2104
NarI	GGCGCC	1 cut(s)	135
NciI	CCSGG	3 cut(s)	332, 2436, 2437
NcoI	CCATGG	1 cut(s)	514
NdeI	CATATG	1 cut(s)	2524
NdeII	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
NlaIV	GGNNCC	6 cut(s)	136, 438, 880, 1039, 1239, 2294
NmeAIII	GCCGAG	1 cut(s)	1899
NmuCI	GTSAC	5 cut(s)	199, 388, 824, 1677, 1974
NsbI	TGCGCA	1 cut(s)	187
NsiI	ATGCAT	3 cut(s)	730, 837, 2530
NspI	RCATGY	4 cut(s)	545, 574, 866, 2266
PaeI	GCATGC	2 cut(s)	545, 866
PagI	TCATGA	1 cut(s)	870
PaqCI	CACCTGC	1 cut(s)	247
PciI	ACATGT	2 cut(s)	570, 2262
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	6 cut(s)	427, 557, 1615, 1648, 1861, 2452
PdmI	GAANNNNTTC	2 cut(s)	555, 2396
PfeI	GAWTC	8 cut(s)	322, 404, 1082, 1192, 1253, 1547, 1766, 1846
PflMI	CCANNNNNTGG	1 cut(s)	1074
PkrI	GCNGC	5 cut(s)	38, 195, 210, 540, 675
PleI	GAGTC	6 cut(s)	961, 1337, 1390, 1685, 2005, 2038
PluTI	GGCGCC	1 cut(s)	138
PpsI	GAGTC	6 cut(s)	961, 1337, 1390, 1685, 2005, 2038
PpuMI	RGGWCCY	1 cut(s)	2292
PscI	ACATGT	2 cut(s)	570, 2262
Psp124BI	GAGCTC	1 cut(s)	762
Psp5II	RGGWCCY	1 cut(s)	2292
Psp6I	CCWGG	3 cut(s)	1208, 1312, 2363
PspGI	CCWGG	3 cut(s)	1208, 1312, 2363
PspN4I	GGNNCC	6 cut(s)	136, 438, 880, 1039, 1239, 2294
PspPI	GGNCC	3 cut(s)	191, 1656, 2292
PspPPI	RGGWCCY	1 cut(s)	2292
PstNI	CAGNNNCTG	2 cut(s)	368, 1967
PsuI	RGATCY	1 cut(s)	1963
RsaI	GTAC	3 cut(s)	471, 1228, 2173
RsaNI	GTAC	3 cut(s)	470, 1227, 2172
RseI	CAYNNNNRTG	5 cut(s)	546, 621, 840, 1803, 1983
SacI	GAGCTC	1 cut(s)	762
SalI	GTCGAC	1 cut(s)	268
SaqAI	TTAA	6 cut(s)	491, 533, 740, 960, 990, 1101
SatI	GCNGC	5 cut(s)	37, 194, 209, 539, 674
Sau3AI	GATC	6 cut(s)	286, 1579, 1963, 2215, 2313, 2359
Sau96I	GGNCC	3 cut(s)	191, 1656, 2292
SchI	GAGTC	6 cut(s)	962, 1338, 1391, 1685, 2006, 2039
ScrFI	CCNGG	6 cut(s)	332, 1210, 1314, 2365, 2436, 2437
SduI	GDGCHC	4 cut(s)	762, 1170, 1800, 2063
SfcI	CTRYAG	1 cut(s)	975
SfoI	GGCGCC	1 cut(s)	136
SgrAI	CRCCGGYG	1 cut(s)	98
SinI	GGWCC	2 cut(s)	1656, 2292
SmaI	CCCGGG	1 cut(s)	2437
SmiMI	CAYNNNNRTG	5 cut(s)	546, 621, 840, 1803, 1983
SmlI	CTYRAG	3 cut(s)	240, 1100, 1357
SmoI	CTYRAG	3 cut(s)	240, 1100, 1357
SphI	GCATGC	2 cut(s)	545, 866
Sse9I	AATT	9 cut(s)	31, 1044, 1346, 1600, 1741, 1810, 2255, 2305, 2372
SsiI	CCGC	9 cut(s)	36, 194, 209, 538, 855, 918, 1170, 1373, 2480
SspDI	GGCGCC	1 cut(s)	134
SspMI	CTAG	5 cut(s)	1269, 1746, 1892, 2163, 2201
SstI	GAGCTC	1 cut(s)	762
StyD4I	CCNGG	6 cut(s)	330, 1208, 1312, 2363, 2434, 2435
StyI	CCWWGG	4 cut(s)	514, 905, 1154, 2175
TaaI	ACNGT	6 cut(s)	127, 205, 462, 830, 892, 1419
TaiI	ACGT	4 cut(s)	8, 226, 781, 1711
TaqI	TCGA	6 cut(s)	20, 252, 269, 317, 1421, 1578
TasI	AATT	9 cut(s)	31, 1044, 1346, 1600, 1741, 1810, 2255, 2305, 2372
TatI	WGTACW	1 cut(s)	469
TauI	GCSGC	4 cut(s)	39, 196, 211, 541
TfiI	GAWTC	8 cut(s)	322, 404, 1082, 1192, 1253, 1547, 1766, 1846
Tru1I	TTAA	6 cut(s)	491, 533, 740, 960, 990, 1101
Tru9I	TTAA	6 cut(s)	491, 533, 740, 960, 990, 1101
TscAI	CASTG	7 cut(s)	306, 373, 534, 570, 895, 1194, 1626
TseFI	GTSAC	5 cut(s)	199, 388, 824, 1677, 1974
TseI	GCWGC	1 cut(s)	673
Tsp45I	GTSAC	5 cut(s)	199, 388, 824, 1677, 1974
TspGWI	ACGGA	4 cut(s)	129, 182, 676, 752
TspMI	CCCGGG	1 cut(s)	2435
TspRI	CASTG	7 cut(s)	306, 373, 534, 570, 895, 1194, 1626
Van91I	CCANNNNNTGG	1 cut(s)	1074
Vha464I	CTTAAG	1 cut(s)	1100
VpaK11BI	GGWCC	2 cut(s)	1656, 2292
XagI	CCTNNNNNAGG	1 cut(s)	1492
XapI	RAATTY	2 cut(s)	1741, 2372
XbaI	TCTAGA	1 cut(s)	1745
XceI	RCATGY	4 cut(s)	545, 574, 866, 2266
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1048, 1809
XmaI	CCCGGG	1 cut(s)	2435
XmiI	GTMKAC	4 cut(s)	269, 894, 1299, 2321
XmnI	GAANNNNTTC	2 cut(s)	555, 2396
XspI	CTAG	5 cut(s)	1269, 1746, 1892, 2163, 2201
ZraI	GACGTC	2 cut(s)	6, 1709
Zsp2I	ATGCAT	3 cut(s)	730, 837, 2530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.