Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011462

Physical Location & Seq

Genomic Coordinates Reverse (-)

30080831 .. 30088073

Gene Structure 7243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1159300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4404 bp

ATGGCGGCTTCTTCTGGGTTGGTTGCAGAGGCGAACCCGGAAGTGTTTCAATGGCTCAAAACCCTACCCGTAGCTCCCGAGTACCACCCCACTTGGGCGGAGTTCCAAGATCCGATTGCCTACATTTTCAAAATCGAGAAGGAGGCTTCACAGTACGGAATCTGCAAAATCGTGCCGCCGGTGCCGCCTTCCTCAAAGAAGACCACAATTGGGAACTTGAACCGGTCTCTGGCTGCTCGGGCGGGGGTTTTGGGTTCTTCGGGCCAGAAATCCCAGCCCACATTCACTACCCGGCAGCAGCAGATCGGGTTCTGCCCTCGGAGGCCACGGCCCGTGCACCGACCCGTGTGGCAGAGCGGCGAGCACTACACTTTCCAGGAATTTGAAGCGAAAGCTAAGAGCTTTGAGAAGAGTTACTTGAGAAAATGTAGCAAGAAGGGAGGGCTTTCGCCGCTGGAAATTGAAACCCTTTACTGGAAGGCGACTGTGGATAAACCCTTTTCGGTGGAGTATGCGAACGACATGCCCGGTTCAGCTTTTGTGCCAGTGAGTGCGAGGAAAAGTAGTAGTACTAGTAAAGATGCAGGGGACAATGTGACTCTTGGTGATACTGCTTGGAATATGAGGGGGGTGTCGAGGTCAAAAGGGTCGTTATTGAGGTTTATGAAGGAGGAGATTCCGGGGGTTACTTCGCCGATGGTGTATATAGCTATGTTGTTCAGCTGGTTTGCTTGGCATGTGGAGGACCATGACTTGCACAGCTTGAATTATCTGCATATGGGAGCAGGGAAGACGTGGTATGGTGTGCCGAGGGACGCTGCGGTTGCATTCGAGGAGGTTGTTAGGGTACAGGGCTATGCAGGAGAGATCAATCCTCTTGTTACCTTTTCAACCCTTGGCGAGAAGACTACTGTGATGTCGCCTGAAGTATTTGTCAGTTCAGGAATTCCATGCTGCAGGCTTGTGCAAAATGCTGGGGAGTTTGTTGTGACTTTCCCAAGAGCGTATCACACTGGATTCAGTCATGGATTTAATTTTGCGGAGGCAGCTAACATTGCAACTCCTGAGTGGTTGAGGGTTGCAAAAGATGCTGCTATTCGACGAGCTTCAATCAATTATCCTCCAATGGTGTCTCATTTTCAGTTACTTTATGATCTTGCTTTAGCATTGTGTTCAAGAATGCCTGCGCGCATAAGTTCTGAACCACGGAGTTCTCGACTGAAAGATAAGAGAAAGGGTGAAGGAGACATAGTAGTTAAAGAGCTATTTGTACAGGATGTCATTCAGAATAATGACCTGCTTCATGTTCTTGGAAAAGGGTCACCAATTGTCCTTCTACCCCAAAGTTCTTCAGACCTTTCTGTTTGTTCAAAATTACGTGTTGGTTCACAATCAAGAGTAAACCCTGGCTTTTCCCAGGGCTCATACAATCTGAAGGAAGAAATGAAATCCTCAGGTTCAGTTTCTGATGGTCTCATGATAGACGGGCAGCAGGTTAAAGGTTTCTATTCCATGAGAGGAAAACTTGCATCTCTAAGTGAAAGTAATAGTCTTCCCTCTTTAAGTGGATGTAATAATGTCTGCGGCCTGAATTTAAAGAGGTCGAACTTGAGCTGTGAAAGAGAAAGTCATGTCGAAGGGGAGGGGTTGTCAGATCAGAGACTGTTTTCTTGTGTTACATGTGGAATTTTGAGCTTTGCTTGTGTTGCTATTATTCAACCAACAGAAGAAGCAGCCAGATATCTTATGTCAGCAGATTGTAGCTTTTTCAATGATTGGGTTGTTGGATCTGGATTGGGTAGTAAGGCGTTCTCGGTTGTTACTGGAGATCCAGTTACTTCAAATGATGCTCCTTGCACAGGATTGGAGGAAAACAATGCCCCTAATGGTTTATATGATGTGCCTGTTCACTCTGGTAATTATCAAATCCAGGTGGTTGATCAAAGAAATGAAGTGGTTTCAAATACTGAAATGCCGAGAGAGACTTCAGCTCTTGGTCTATTAGCTTTGAATTATGGAAATTCATCTGACTTGGAGGAAGATCAGGTTGAAACAGATGCTCCTGTCTGTTCTGATGAACCAAAGGTGACAAACTGTTCTTTGGAAAGTAGATATAGAGACCAAAGTGCTAGTCTTCCTTCTGGGGGTACAAGTGGGGTTCACAGTCCCTCGTCACCAGGATCTGATTGTGAGAATGAACTTCGTCTCCAAAATTTTGATCACTATGCTACAGATGGACGTAAAATAGCAAATTTCAAGGATACCGGCCATCAAGACATTGACAGCTCTGCTGATTTCAGAACTAATAATTATGCTTCCACAGCAACAGGTTTTGGTAAGGCTATCGTACCAATACAGAAAACAAACACGTCATGTCATCCAGGATGCGATGAAGACTCTTCTAGAATGCATGTCTTCTGCCTTGAGCATGCTGTAGAGGTTCAGCAGCAGCTTCGTTCAATTGGAGGTGTCCATATTTTACTCCTTTGTCATCCAGACTACCCAAGGATAGAGGATGAAGCCAAATCAATGGCAGAAGAGCTGGGAATAAGTTATCTCTGGAATGATATGGCATTCATGAATGCCACCAAGGAAGATGAGACGAGGATTAAGTTAGCTCTGGATAGCGAGGATGCCATTGCTGGGAATGGGGACTGGGCTGTAAAGTTGGGGATCAATCTCTTCTACAGTGCCAGCCTTAGCCGCTCTCATCTTTATAGTAAGCAGATGCCATACAATTCTGTTATATACAAAGCATTTGGCCGGAGCTCTCCTGCTAGTTCGCCAACAAGAATTGATGTCTATGGGAGGAGAGGTGGCAAGCCAAAAAAGGTAGTGGCAGGGAAATGGTGTGGTAAAGTATGGATGTCAAACCAAGTTCATTCTTTTCTAGTAAAAAGGGATCCTGAGGAAGAAGTAGAAGTAGCAGAAGATGAAGAAGAGCGGCCCTTCCGTGCTTGGGCAATGCCAGAAGAGGATGATGAGGTAAAATCAGAAATCACACGCAGAACTGAGAAGACAGTTAAGAAGTATGCTAGGAAGAGGAAAATGACTGCAGATACTCGGACAGCCAAGAAAGCAAAATGTTTTGAGAAAGAGGATGCAGTTTCAGATTATTCATTAGATGACAACTCTCATCAGCAGCAAAGGAGGCTTCCTAAGAGCAAGCAAGCCAAACATATTGAGAGAGGTCGAACCAAGAAAGCCAAGGGTATTGAGACACATGATGCATTTTCAGATGATACAATGCAAGAGGATGATTCTCATCAGCAAAATGGGAGGATTCTTCACAGCGAGCAAGTTGAGTATATTGAGAGAAGTGATGTTTCAGATGATTCTGTGGGAATTGATTCTCATCAGCAGCATAGGAGGACTGCTAAAAGTAAGCAATTCAAGCCAGTAGAAACAGATGTGGTTTCAGATGATTCGTTTGAGGGTAGTTCTCATCAGCCGCGGAGGGTCCTTAGAAGCAAGACAACTAAATGCACTGGGAGAGAAAATTTGATTTCGGAGGATGTTCGCGGGTTTAGTTCTCATCAGCAACGAAGGAGCATTTCTAGAAGCAAGCAGGCCAGAGCCAGATTCATGGAGAGAGAAGATACCTCTCTGGACGAAACACCGGAAGATACTTCTCATCAGCATAAGAGAAATCTTCGAAACAAGCAAACCAAACCAGAGACCCGTGGGAAAATGAGACAAGAAACCTCTCGCCAAGTGAAACAAGGAACTGCTCCTCTTGTGAAACAAGGTACTCGCACTCTGAGAAATCAACAAACCCCTCAGAAAACGAAGAAGCAGACACCTAGGCTCCGAAATAATCAATCTGAGCAGAATAATCAGTCTGAGCAGAACAGCTTTGATTTATATGCTGAAGATGAGACAGAAGGTGGGCCTAGCACGCGCCTTAGAAAAAGAGCTCCTAAGCCCTCAAAAGTCCCTGGAACCAAATCAAAAGAGCAGCAACAAACTGCTAGGAAAAAGGCAAAGAATGCTTCAGCAGGGAAGACTCAGGCTGGCCGAAATGAAACAAAGAGGGAAGAGGATGGAGAATTCGTGTGTGATCTTGAGGGGTGTACCATGAGTTTTGCCTCGAGGCATGAGCTTTCCCTCCACAAAAGAAACATATGTCCAGTCAAGGGGTGCGGAAAGAAATTCTTCTCGCACAAGTATCTGGTGCAACATCGCCGAGTTCACACAGATGATCGCCCCCTTCGGTGTCCTTGGAAGGGCTGCAAGATGACATTCAAGTGGGCATGGTCTCGCACCGAGCACATTAGGGTTCACACAGGTGCTCGTCCCTATGTATGCGCTGAACCAGGGTGTGCACAGACCTTCCGATTTGTGTCAGATTTCAGCCGTCATAAGCGCAAGACCGGGCATTCAGCGAAGAAGAGTAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010605 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0099402 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

JmjC JmjN zf-C2H2

Protein Analysis

1468

Amino Acids

164.0

Weight (kDa)

8.87

Isoelectric Point (pI)

55.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjN	PF02375	26 - 59	2.9e-15	jmjN domain
JmjC	PF02373	234 - 351	1.4e-34	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48430 AT3G48430
fragaria_vesca	FvH4_5g07090 FvH4_5g07090 FvH4_5g07090
malus_domestica	MD06G1159300.v1.1 MD14G1165600.v1.1
prunus_persica	Prupe.5G159200_v2.0.a1
pyrus_communis	pycom06g14210 pycom14g13810
rosa_chinensis	RchiOBHm_Chr7g0191461
rosa_laevigata	RLG00000004448
rosa_multiflora	Rmu_sc0002661.1_g000009
rosa_roxburghii	Rroxscaffold_157G00438400 Rroxscaffold_3G00263790
rosa_rugosa	Rorug06G0510400
rosa_samantha	Rh7AG118500 Rh7BG120500 Rh7CG123300 Rh7DG121400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	4090
Acc36I	ACCTGC	2 cut(s)	1305, 1483
AccB1I	GGYRCC	1 cut(s)	181
AccBSI	CCGCTC	3 cut(s)	357, 2706, 2944
AccII	CGCG	4 cut(s)	1189, 3454, 3522, 3901
AclWI	GGATC	7 cut(s)	104, 1795, 1823, 2188, 2681, 2897, 2910
AcoI	YGGCCR	3 cut(s)	2266, 2761, 4015
AcuI	CTGAAG	6 cut(s)	945, 1335, 1454, 1971, 3891, 3978
AfaI	GTAC	9 cut(s)	83, 155, 571, 849, 1272, 2149, 2349, 3751, 4075
AflIII	ACRYGT	3 cut(s)	1378, 1679, 2367
AgeI	ACCGGT	1 cut(s)	222
AhlI	ACTAGT	1 cut(s)	572
AjiI	CACGTC	2 cut(s)	795, 2370
AjnI	CCWGG	8 cut(s)	375, 1405, 1416, 1929, 2176, 2380, 3937, 4315
AleI	CACNNNNGTG	1 cut(s)	4287
AloI	GAACNNNNNNTCC	2 cut(s)	2190, 2222
Alw21I	GWGCWC	7 cut(s)	339, 366, 2771, 3919, 4272, 4294, 4327
Alw44I	GTGCAC	2 cut(s)	335, 4323
AlwI	GGATC	7 cut(s)	104, 1795, 1823, 2188, 2681, 2897, 2910
AlwNI	CAGNNNCTG	3 cut(s)	1466, 1663, 2183
Ama87I	CYCGRG	3 cut(s)	77, 237, 4090
ApaLI	GTGCAC	2 cut(s)	335, 4323
ArsI	GACNNNNNNTTYG	2 cut(s)	2116, 2148
AsiGI	ACCGGT	1 cut(s)	222
Asp700I	GAANNNNTTC	3 cut(s)	45, 3349, 4154
AspA2I	CCTAGG	1 cut(s)	3803
AspLEI	GCGC	5 cut(s)	1189, 1191, 3903, 4310, 4368
AspS9I	GGNCC	6 cut(s)	262, 330, 745, 2946, 3460, 3890
AsuC2I	CCSGG	5 cut(s)	38, 292, 528, 681, 4375
AsuHPI	GGTGA	5 cut(s)	617, 1250, 1314, 2098, 2166
AsuII	TTCGAA	1 cut(s)	3655
AvaI	CYCGRG	3 cut(s)	77, 237, 4090
AvaII	GGWCC	2 cut(s)	745, 3460
AvrII	CCTAGG	1 cut(s)	3803
AxyI	CCTNAGG	2 cut(s)	1453, 2907
BaeGI	GKGCMC	2 cut(s)	339, 4327
BamHI	GGATCC	1 cut(s)	2902
BanI	GGYRCC	1 cut(s)	181
BanII	GRGCYC	3 cut(s)	1424, 2771, 3919
BbsI	GAAGAC	9 cut(s)	206, 797, 911, 1544, 2126, 2400, 2407, 3023, 4010
Bbv12I	GWGCWC	7 cut(s)	339, 366, 2771, 3919, 4272, 4294, 4327
BccI	CCATC	5 cut(s)	691, 1463, 2228, 2277, 4037
BceAI	ACGGC	2 cut(s)	344, 4341
BcgI	CGANNNNNNTGC	4 cut(s)	505, 539, 2435, 2469
BciT130I	CCWGG	8 cut(s)	377, 1407, 1418, 1931, 2178, 2382, 3939, 4317
BciVI	GTATCC	1 cut(s)	2254
BclI	TGATCA	2 cut(s)	1939, 2218
BcnI	CCSGG	5 cut(s)	38, 292, 528, 681, 4375
BcuI	ACTAGT	1 cut(s)	572
BfmI	CTRYAG	5 cut(s)	955, 2229, 2433, 2686, 3054
BfuAI	ACCTGC	2 cut(s)	1305, 1483
BfuI	GTATCC	1 cut(s)	2254
BlnI	CCTAGG	1 cut(s)	3803
BmcAI	AGTACT	1 cut(s)	571
Bme18I	GGWCC	2 cut(s)	745, 3460
BmeT110I	CYCGRG	3 cut(s)	77, 237, 4090
BmgBI	CACGTC	2 cut(s)	795, 2370
BmgT120I	GGNCC	6 cut(s)	262, 330, 745, 2946, 3460, 3890
BmiI	GGNNCC	5 cut(s)	183, 2904, 3461, 3809, 3943
BmrI	ACTGGG	2 cut(s)	2665, 3498
BmuI	ACTGGG	2 cut(s)	2665, 3498
BpiI	GAAGAC	9 cut(s)	206, 797, 911, 1544, 2126, 2400, 2407, 3023, 4010
BpmI	CTGGAG	1 cut(s)	1845
Bpu10I	CCTNAGC	2 cut(s)	2699, 3921
Bpu14I	TTCGAA	1 cut(s)	3655
BpuEI	CTTGAG	4 cut(s)	439, 1630, 2444, 4085
BpuMI	CCSGG	5 cut(s)	38, 292, 528, 681, 4375
BsaAI	YACGTR	1 cut(s)	1379
BsaBI	GATNNNNATC	2 cut(s)	3264, 3354
BsaI	GGTCTC	5 cut(s)	231, 1478, 2112, 3671, 4263
BsaWI	WCCGGW	2 cut(s)	222, 3619
Bse118I	RCCGGY	3 cut(s)	178, 222, 2264
Bse1I	ACTGG	9 cut(s)	479, 545, 1018, 1828, 1832, 2660, 3398, 3493, 4130
Bse21I	CCTNAGG	2 cut(s)	1453, 2907
Bse3DI	GCAATG	3 cut(s)	1053, 2637, 2970
Bse8I	GATNNNNATC	2 cut(s)	3264, 3354
BseBI	CCWGG	8 cut(s)	377, 1407, 1418, 1931, 2178, 2382, 3939, 4317
BseJI	GATNNNNATC	2 cut(s)	3264, 3354
BseMI	GCAATG	3 cut(s)	1053, 2637, 2970
BseMII	CTCAG	9 cut(s)	1056, 1467, 2898, 3003, 3752, 3794, 3816, 3834, 4022
BseNI	ACTGG	9 cut(s)	479, 545, 1018, 1828, 1832, 2660, 3398, 3493, 4130
BsePI	GCGCGC	1 cut(s)	1187
BseRI	GAGGAG	4 cut(s)	686, 848, 2824, 3723
BseSI	GKGCMC	2 cut(s)	339, 4327
BseYI	CCCAGC	4 cut(s)	273, 974, 2542, 2642
Bsh1236I	CGCG	4 cut(s)	1189, 3454, 3522, 3901
BshNI	GGYRCC	1 cut(s)	181
BshTI	ACCGGT	1 cut(s)	222
BsiHKAI	GWGCWC	7 cut(s)	339, 366, 2771, 3919, 4272, 4294, 4327
BsiHKCI	CYCGRG	3 cut(s)	77, 237, 4090
BslFI	GGGAC	6 cut(s)	602, 827, 2151, 2666, 3920, 4281
BsmBI	CGTCTC	2 cut(s)	2210, 2594
BsmFI	GGGAC	6 cut(s)	602, 827, 2151, 2666, 3920, 4281
BsmI	GAATGC	7 cut(s)	827, 1185, 2412, 2573, 2587, 3994, 4378
Bso31I	GGTCTC	5 cut(s)	231, 1478, 2112, 3671, 4263
BsoBI	CYCGRG	3 cut(s)	77, 237, 4090
Bsp119I	TTCGAA	1 cut(s)	3655
Bsp1286I	GDGCHC	8 cut(s)	339, 366, 1424, 2771, 3919, 4272, 4294, 4327
Bsp1407I	TGTACA	1 cut(s)	1270
BspCNI	CTCAG	9 cut(s)	1057, 1466, 2899, 3004, 3753, 3793, 3817, 3835, 4021
BspFNI	CGCG	4 cut(s)	1189, 3454, 3522, 3901
BspHI	TCATGA	2 cut(s)	1476, 2577
BspLI	GGNNCC	5 cut(s)	183, 2904, 3461, 3809, 3943
BspMAI	CTGCAG	2 cut(s)	959, 3058
BspMI	ACCTGC	2 cut(s)	1305, 1483
BspPI	GGATC	7 cut(s)	104, 1795, 1823, 2188, 2681, 2897, 2910
BspQI	GCTCTTC	2 cut(s)	2532, 2934
BspT104I	TTCGAA	1 cut(s)	3655
BspT107I	GGYRCC	1 cut(s)	181
BspTNI	GGTCTC	5 cut(s)	231, 1478, 2112, 3671, 4263
BsrBI	CCGCTC	3 cut(s)	357, 2706, 2944
BsrDI	GCAATG	3 cut(s)	1053, 2637, 2970
BsrFI	RCCGGY	3 cut(s)	178, 222, 2264
BsrGI	TGTACA	1 cut(s)	1270
BsrI	ACTGG	9 cut(s)	479, 545, 1018, 1828, 1832, 2660, 3398, 3493, 4130
BssAI	RCCGGY	3 cut(s)	178, 222, 2264
BssHII	GCGCGC	1 cut(s)	1187
BssT1I	CCWWGG	6 cut(s)	895, 2504, 2589, 3207, 3803, 4220
Bst2UI	CCWGG	8 cut(s)	377, 1407, 1418, 1931, 2178, 2382, 3939, 4317
Bst4CI	ACNGT	8 cut(s)	153, 487, 913, 1665, 2096, 2165, 2690, 3022
Bst6I	CTCTTC	9 cut(s)	404, 2404, 2532, 2687, 2934, 2967, 3035, 4032, 4385
BstAPI	GCANNNNNTGC	2 cut(s)	1088, 3989
BstAUI	TGTACA	1 cut(s)	1270
BstBAI	YACGTR	1 cut(s)	1379
BstBI	TTCGAA	1 cut(s)	3655
BstDSI	CCRYGG	4 cut(s)	326, 1205, 3452, 3682
BstEII	GGTNACC	1 cut(s)	1320
BstENI	CCTNNNNNAGG	1 cut(s)	1857
BstFNI	CGCG	4 cut(s)	1189, 3454, 3522, 3901
BstHHI	GCGC	5 cut(s)	1189, 1191, 3903, 4310, 4368
BstNI	CCWGG	8 cut(s)	377, 1407, 1418, 1931, 2178, 2382, 3939, 4317
BstNSI	RCATGY	5 cut(s)	526, 740, 1683, 2414, 2432
BstPI	GGTNACC	1 cut(s)	1320
BstSFI	CTRYAG	5 cut(s)	955, 2229, 2433, 2686, 3054
BstSLI	GKGCMC	2 cut(s)	339, 4327
BstUI	CGCG	4 cut(s)	1189, 3454, 3522, 3901
BstV2I	GAAGAC	9 cut(s)	206, 797, 911, 1544, 2126, 2400, 2407, 3023, 4010
BstX2I	RGATCY	5 cut(s)	109, 1787, 1828, 2180, 2902
BstXI	CCANNNNNNTGG	2 cut(s)	2530, 3586
BstYI	RGATCY	5 cut(s)	109, 1787, 1828, 2180, 2902
Bsu36I	CCTNAGG	2 cut(s)	1453, 2907
BsuI	GTATCC	1 cut(s)	2254
BtgI	CCRYGG	4 cut(s)	326, 1205, 3452, 3682
BtgZI	GCGATG	2 cut(s)	2403, 4166
BtrI	CACGTC	2 cut(s)	795, 2370
BtsIMutI	CAGTG	4 cut(s)	552, 1011, 2695, 3486
BveI	ACCTGC	2 cut(s)	1305, 1483
CaiI	CAGNNNCTG	3 cut(s)	1466, 1663, 2183
CciI	TCATGA	2 cut(s)	1476, 2577
CfoI	GCGC	5 cut(s)	1189, 1191, 3903, 4310, 4368
Cfr10I	RCCGGY	3 cut(s)	178, 222, 2264
Cfr13I	GGNCC	6 cut(s)	262, 330, 745, 2946, 3460, 3890
Cfr42I	CCGCGG	1 cut(s)	3455
CseI	GACGC	1 cut(s)	824
Csp6I	GTAC	9 cut(s)	82, 154, 570, 848, 1271, 2148, 2348, 3750, 4074
CspAI	ACCGGT	1 cut(s)	222
CspCI	CAANNNNNGTGG	2 cut(s)	74, 109
CviQI	GTAC	9 cut(s)	82, 154, 570, 848, 1271, 2148, 2348, 3750, 4074
DraI	TTTAAA	1 cut(s)	1596
EaeI	YGGCCR	3 cut(s)	2266, 2761, 4015
Eam1104I	CTCTTC	9 cut(s)	404, 2404, 2532, 2687, 2934, 2967, 3035, 4032, 4385
EarI	CTCTTC	9 cut(s)	404, 2404, 2532, 2687, 2934, 2967, 3035, 4032, 4385
EciI	GGCGGA	1 cut(s)	113
Ecl136II	GAGCTC	2 cut(s)	2769, 3917
Eco130I	CCWWGG	6 cut(s)	895, 2504, 2589, 3207, 3803, 4220
Eco24I	GRGCYC	3 cut(s)	1424, 2771, 3919
Eco31I	GGTCTC	5 cut(s)	231, 1478, 2112, 3671, 4263
Eco32I	GATATC	1 cut(s)	1742
Eco47I	GGWCC	2 cut(s)	745, 3460
Eco53kI	GAGCTC	2 cut(s)	2769, 3917
Eco57I	CTGAAG	6 cut(s)	945, 1335, 1454, 1971, 3891, 3978
Eco81I	CCTNAGG	2 cut(s)	1453, 2907
Eco88I	CYCGRG	3 cut(s)	77, 237, 4090
Eco91I	GGTNACC	1 cut(s)	1320
EcoICRI	GAGCTC	2 cut(s)	2769, 3917
EcoNI	CCTNNNNNAGG	1 cut(s)	1857
EcoO109I	RGGNCCY	1 cut(s)	3460
EcoO65I	GGTNACC	1 cut(s)	1320
EcoRI	GAATTC	2 cut(s)	945, 4049
EcoRII	CCWGG	8 cut(s)	375, 1405, 1416, 1929, 2176, 2380, 3937, 4315
EcoRV	GATATC	1 cut(s)	1742
EcoT14I	CCWWGG	6 cut(s)	895, 2504, 2589, 3207, 3803, 4220
EcoT22I	ATGCAT	2 cut(s)	2412, 3232
EcoT38I	GRGCYC	3 cut(s)	1424, 2771, 3919
ErhI	CCWWGG	6 cut(s)	895, 2504, 2589, 3207, 3803, 4220
Esp3I	CGTCTC	2 cut(s)	2210, 2594
FalI	AAGNNNNNCTT	4 cut(s)	401, 433, 4139, 4171
FaqI	GGGAC	6 cut(s)	602, 827, 2151, 2666, 3920, 4281
FauI	CCCGC	2 cut(s)	235, 3515
FauNDI	CATATG	2 cut(s)	777, 4124
FbaI	TGATCA	2 cut(s)	1939, 2218
FriOI	GRGCYC	3 cut(s)	1424, 2771, 3919
GlaI	GCGC	5 cut(s)	1188, 1190, 3902, 4309, 4367
GsaI	CCCAGC	4 cut(s)	277, 978, 2546, 2646
GsuI	CTGGAG	1 cut(s)	1845
HgaI	GACGC	1 cut(s)	824
HhaI	GCGC	5 cut(s)	1189, 1191, 3903, 4310, 4368
Hin6I	GCGC	5 cut(s)	1187, 1189, 3901, 4308, 4366
HinP1I	GCGC	5 cut(s)	1187, 1189, 3901, 4308, 4366
HphI	GGTGA	5 cut(s)	617, 1250, 1314, 2098, 2166
Hpy166II	GTNNAC	9 cut(s)	337, 1388, 1402, 1909, 2161, 4074, 4192, 4282, 4325
Hpy8I	GTNNAC	9 cut(s)	337, 1388, 1402, 1909, 2161, 4074, 4192, 4282, 4325
Hpy99I	CGWCG	1 cut(s)	1104
HpyCH4III	ACNGT	8 cut(s)	153, 487, 913, 1665, 2096, 2165, 2690, 3022
HpyCH4IV	ACGT	4 cut(s)	794, 1378, 2239, 2369
HpySE526I	ACGT	4 cut(s)	794, 1378, 2239, 2369
HspAI	GCGC	5 cut(s)	1187, 1189, 3901, 4308, 4366
Ksp22I	TGATCA	2 cut(s)	1939, 2218
KspI	CCGCGG	1 cut(s)	3455
LguI	GCTCTTC	2 cut(s)	2532, 2934
LmnI	GCTCC	9 cut(s)	79, 782, 1855, 2065, 2766, 3549, 3736, 3813, 3922
MaeII	ACGT	4 cut(s)	794, 1378, 2239, 2369
MbiI	CCGCTC	3 cut(s)	357, 2706, 2944
MfeI	CAATTG	3 cut(s)	207, 1326, 2460
MflI	RGATCY	5 cut(s)	109, 1787, 1828, 2180, 2902
MhlI	GDGCHC	8 cut(s)	339, 366, 1424, 2771, 3919, 4272, 4294, 4327
MlyI	GAGTC	3 cut(s)	592, 2390, 4000
MmeI	TCCRAC	1 cut(s)	1765
Mph1103I	ATGCAT	2 cut(s)	2412, 3232
MroXI	GAANNNNTTC	3 cut(s)	45, 3349, 4154
MseI	TTAA	7 cut(s)	1032, 1257, 1497, 1562, 1595, 2610, 3024
MslI	CAYNNNNRTG	3 cut(s)	4197, 4246, 4287
MspA1I	CMGCKG	3 cut(s)	454, 723, 3454
MunI	CAATTG	3 cut(s)	207, 1326, 2460
Mva1269I	GAATGC	7 cut(s)	827, 1185, 2412, 2573, 2587, 3994, 4378
MvaI	CCWGG	8 cut(s)	377, 1407, 1418, 1931, 2178, 2382, 3939, 4317
MvnI	CGCG	4 cut(s)	1189, 3454, 3522, 3901
NciI	CCSGG	5 cut(s)	38, 292, 528, 681, 4375
NdeI	CATATG	2 cut(s)	777, 4124
NlaIV	GGNNCC	5 cut(s)	183, 2904, 3461, 3809, 3943
NmeAIII	GCCGAG	3 cut(s)	834, 2001, 4211
NmuCI	GTSAC	5 cut(s)	595, 988, 1320, 2086, 2172
NsiI	ATGCAT	2 cut(s)	2412, 3232
NspI	RCATGY	5 cut(s)	526, 740, 1683, 2414, 2432
NspV	TTCGAA	1 cut(s)	3655
OliI	CACNNNNGTG	1 cut(s)	4287
PaeI	GCATGC	1 cut(s)	2432
PaeR7I	CTCGAG	1 cut(s)	4090
PagI	TCATGA	2 cut(s)	1476, 2577
PasI	CCCWGGG	1 cut(s)	1417
PauI	GCGCGC	1 cut(s)	1187
PciI	ACATGT	1 cut(s)	1679
PciSI	GCTCTTC	2 cut(s)	2532, 2934
PcsI	WCGNNNNNNNCGW	1 cut(s)	1213
PctI	GAATGC	7 cut(s)	827, 1185, 2412, 2573, 2587, 3994, 4378
PdmI	GAANNNNTTC	3 cut(s)	45, 3349, 4154
PfeI	GAWTC	9 cut(s)	159, 676, 1017, 3260, 3283, 3335, 3350, 3425, 3582
PfoI	TCCNGGA	2 cut(s)	375, 2380
PinAI	ACCGGT	1 cut(s)	222
PleI	GAGTC	3 cut(s)	592, 2390, 4000
PpsI	GAGTC	3 cut(s)	592, 2390, 4000
Ppu21I	YACGTR	1 cut(s)	1379
PpuMI	RGGWCCY	1 cut(s)	3460
PscI	ACATGT	1 cut(s)	1679
Psp124BI	GAGCTC	2 cut(s)	2771, 3919
Psp5II	RGGWCCY	1 cut(s)	3460
Psp6I	CCWGG	8 cut(s)	375, 1405, 1416, 1929, 2176, 2380, 3937, 4315
PspEI	GGTNACC	1 cut(s)	1320
PspFI	CCCAGC	4 cut(s)	273, 974, 2542, 2642
PspGI	CCWGG	8 cut(s)	375, 1405, 1416, 1929, 2176, 2380, 3937, 4315
PspN4I	GGNNCC	5 cut(s)	183, 2904, 3461, 3809, 3943
PspPI	GGNCC	6 cut(s)	262, 330, 745, 2946, 3460, 3890
PspPPI	RGGWCCY	1 cut(s)	3460
PspXI	VCTCGAGB	1 cut(s)	4090
PstI	CTGCAG	2 cut(s)	959, 3058
PstNI	CAGNNNCTG	3 cut(s)	1466, 1663, 2183
PsuI	RGATCY	5 cut(s)	109, 1787, 1828, 2180, 2902
PteI	GCGCGC	1 cut(s)	1187
PvuII	CAGCTG	1 cut(s)	723
RsaI	GTAC	9 cut(s)	83, 155, 571, 849, 1272, 2149, 2349, 3751, 4075
RsaNI	GTAC	9 cut(s)	82, 154, 570, 848, 1271, 2148, 2348, 3750, 4074
RseI	CAYNNNNRTG	3 cut(s)	4197, 4246, 4287
SacI	GAGCTC	2 cut(s)	2771, 3919
SacII	CCGCGG	1 cut(s)	3455
SapI	GCTCTTC	2 cut(s)	2532, 2934
SaqAI	TTAA	7 cut(s)	1032, 1257, 1497, 1562, 1595, 2610, 3024
Sau96I	GGNCC	6 cut(s)	262, 330, 745, 2946, 3460, 3890
ScaI	AGTACT	1 cut(s)	571
SchI	GAGTC	3 cut(s)	592, 2390, 4000
SduI	GDGCHC	8 cut(s)	339, 366, 1424, 2771, 3919, 4272, 4294, 4327
SfcI	CTRYAG	5 cut(s)	955, 2229, 2433, 2686, 3054
Sfr274I	CTCGAG	1 cut(s)	4090
Sfr303I	CCGCGG	1 cut(s)	3455
SfuI	TTCGAA	1 cut(s)	3655
SgrAI	CRCCGGYG	1 cut(s)	178
SgrBI	CCGCGG	1 cut(s)	3455
SinI	GGWCC	2 cut(s)	745, 3460
SlaI	CTCGAG	1 cut(s)	4090
SmiMI	CAYNNNNRTG	3 cut(s)	4197, 4246, 4287
SmlI	CTYRAG	5 cut(s)	418, 1609, 2423, 4064, 4090
SmoI	CTYRAG	5 cut(s)	418, 1609, 2423, 4064, 4090
SpeI	ACTAGT	1 cut(s)	572
SphI	GCATGC	1 cut(s)	2432
SstI	GAGCTC	2 cut(s)	2771, 3919
StyI	CCWWGG	6 cut(s)	895, 2504, 2589, 3207, 3803, 4220
TaaI	ACNGT	8 cut(s)	153, 487, 913, 1665, 2096, 2165, 2690, 3022
TaiI	ACGT	4 cut(s)	797, 1381, 2242, 2372
TatI	WGTACW	2 cut(s)	569, 1270
TauI	GCSGC	9 cut(s)	8, 178, 187, 360, 454, 1587, 2706, 2947, 3454
TfiI	GAWTC	9 cut(s)	159, 676, 1017, 3260, 3283, 3335, 3350, 3425, 3582
Tru1I	TTAA	7 cut(s)	1032, 1257, 1497, 1562, 1595, 2610, 3024
Tru9I	TTAA	7 cut(s)	1032, 1257, 1497, 1562, 1595, 2610, 3024
TscAI	CASTG	4 cut(s)	552, 1018, 2695, 3493
TseFI	GTSAC	5 cut(s)	595, 988, 1320, 2086, 2172
Tsp45I	GTSAC	5 cut(s)	595, 988, 1320, 2086, 2172
TspGWI	ACGGA	3 cut(s)	171, 1222, 2942
TspRI	CASTG	4 cut(s)	552, 1018, 2695, 3493
VneI	GTGCAC	2 cut(s)	335, 4323
VpaK11BI	GGWCC	2 cut(s)	745, 3460
XagI	CCTNNNNNAGG	1 cut(s)	1857
XbaI	TCTAGA	2 cut(s)	2402, 3557
XceI	RCATGY	5 cut(s)	526, 740, 1683, 2414, 2432
XhoI	CTCGAG	1 cut(s)	4090
XmaJI	CCTAGG	1 cut(s)	3803
XmnI	GAANNNNTTC	3 cut(s)	45, 3349, 4154
ZrmI	AGTACT	1 cut(s)	571
Zsp2I	ATGCAT	2 cut(s)	2412, 3232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.