Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000157

Orthogroup ID

OG0011462

Physical Location & Seq

Genomic Coordinates Reverse (-)

82316 .. 93787

Gene Structure 11472 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_157G00438400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5793 bp

ATGGCAGCTTCGGAGCAACCCCAGGAAGTTCTCTCATGGCTCAGAGCCCTACCCGTAGCTCCGGAGTACCACCCTACTTGGGCCGAGTTCCAAGATCCGATTGCCTACATTTTCAAGATCGAGAAGGAGGCTTCCAAGTATGGAATCTGCAAAATCGTGCCGCCGGTGCCGCCGGCGCCGAAGAAAACAGCAATTGCTAATCTCAACAAGTCTCTGGTGGCGCGTGGTGGGCCTTCGGTTGGAAAAGGCCCGAAAGCCCTGCCCACATTCACTACCCGGCAGCAGCAGATCGGGTTCTGCCCCCGGAAGGCCCGCCCCGTGCAGAGACCCGTGTGGCAGAGCGGCGAGCACTACACATTCAACCAATTCGAGGCCAAAGCCAAGAGCTTTGAGAAGAGCTACTTGAAGAAGCGGAGGAAGAAGGGAGGCCTGAACCCTCTGGATATCGAGACCCTTTACTGGAAAGCCACTGTGGACAAGCCCTTTTCCGTTGAGTACGCCAATGACATGCCCGGCTCGGCTTTCGTGCCGCTGAGCAGCAAGAAGAGTGGTGCCAGCACTAGCAGGGAGGCAGGGGATGGCGTCACGCTGGGAGAGACGGCCTGGAATATGAGGGGGTTTCCAGGGAAGACTTGGTATGGCGTGCCCAGGGAGGCTGCCGTGGCGTTCGAGGAGGTGGTGAGAGTTCAGGGGTATGGAGGAGAGATCAATCCTCTTGTTACATTTGCGACGCTTGGTGAGAAGACCACTGTGATGTCACCTGAAGTCTTTATTAGTTCAGGGATTCCATGCTGCAGGTTAGTGCAAAATGCTGGGGAATTTGTTGTCACTTTTCCAAGAGCCTATCACACTGGATTCAGTCACGGATTTAATTGTGGGGAGGCAGCCAACATTGCAACTCCTGAGTGGTTGAGGGTTGCCAATGATGCTGCTGTCCGACGAGCTTCAATCAATTATCCTCCTATGGTGTCTCATTTTCAGTTACTTTATGATCTTGCTCTAGCATTATGTTCAAGAACGCCTGTGCGTAATAGTGCTGAACCTCGAAGTTCTCGACTAAAAGATAAGAAAAAGGGTGAAGGAGAAACAGTAGTTAAAGGTTTATTTGTGAAGAATGTCATTCAGAACAATGAGCTGCTTCATGTTCTTGGAAAAGGGTCGTCAATTGTACTTCTACCACAGAGTTCTTCAGACATTTCTGTTTGTTCAAAATTACGTGTTGGTTCACAGTTAAGAGTAAACCCTGATGATCTCATGATAGACGGGAAGCAGGGAATCAAGCAGGTAAAAGGGTTGTATTCAGTGAAAGGAAAACTTGCATCTTTATGTGAAAGTAGCCAGCATCCCTCCTTAAATGCAAATGGCAATGCAAGTACTCCTTCTAAGATGTTGAACATGAGCGCTAAAAGAGAAAGTAATGTTGAAGGTGAAGGGTTGTCAGATCAGAGACTATTTTCTTGTGTTACATGTGGAATTTTGAGCTTTTCATGTGTTGCTATAATTCAACCAAGAGAAGCAGCAGCCAGATATCTCATGTCAGCAGATTGTAGTTTTTTCAATGATTGGGCTGTTGATTGTGAGCCTATCCAGGTTGCAAATGGAGATCCAAATAGTTCAAAGAAGGGTCCCTGTACAGAAACAGGATTGATGCAAAAGAGTACCCATGATGGTTTATACGATGTGCCCGTTCAGTCTGCTGATTATCAAAATCAGATCACAGATCCAATCAATGAACAGAGAGATACTAATTCGCTTGGGCTATTAGCTTTGACCTATGGAGATTCATCTGACTCGGAGGAAGATCAGGCTGAACCAGATGTTCCTGTTTGTGGTGACGAAACAAACTTGTCAGACTGTTCTTTGGAAGGTAGATATGAGTATAAAAGTGCTAGTCCTCCTTTGAGAGACTCTTATGGAGGTACAGCTGGAGTTCGCAGTCCAACCTCATCAGGATTTGATTGTGGTAATGAACTCCCGACTGTTGATGGCAATGTGGAAAATAGACGTGAAGCAACTAATTTCAAGGATAATGGTCATCAATATATTGACTGTTCTGTTGATCTTGACACAAATAATCTTGCTTTAACAAAGACAAATGGTTTGGCGGGTACATCTATAGATCCAGTAAAGGTTTCATATTCTGGCTCCCCAGATGCGTTTGATGTTCAACTAACACAATTTGGTCAGGCCACTTTACGGAAAGAGAGTACAGGCACATCATTTTTTCCAGGATGTGACCAGGATTCTTCTAGAATGCATGTGTTCTGCCTTGAGCATGCTGTAGAGGTTGAGCAGCAGCTCCGTTCATTTGGAGGTGCCCATATATTACTCCTTTGTCATCCAGCACATTCTGTAAGTAGCAGGAATATGGGCCGTTCTGCTGCTTATGTTTTAGTTATATTTTCTGAACTTTATGCCTATATGGAACGGTGGAGGCACCATTGCTTAGACTATCCGCGGATAGTGGATGAAGCAAAAGAAATGGCAGAAGAACTGGGAGTAAATTATCCCTGGAATGATATGGTATTCAGGGATGCCACCAGGGAGGATGAGGAGAGGATTCAGTCAGCACTGGATAGTGAGGAAGCCATTGCTGGGAATGGAGACTGGGCTGTAAAGATGGGGATCAATCTCTTCTACAGTGCCAGCCTTAGCCGCTCTCATCTTTATAGTAAGCAGATGCCATACAATTCTGTTATATACAATGCATTTGGTCGTAGTTCTCCAGCTAGCTCGCCTGCAGGACCTGAGGTCTGTGGGAGGAGGCCTGCCAAACAGAAAAAGATGGTGGTAGGGAAATGGTGCGGTAAAGTTTGGATGTCAAATCAAGTTCATCCCTTCTTAATAAAAAGGGATCATGAGGAGAAAAAAGTAGAGCTAGAACAGAGGAGATTCCATGATTCAGCAATGCCAGATGAGAAGATTGATGGAAAATCAGAAAGCACACGCAAAACTGAGAAAACTATGGTGACTAAACAGTATTCTAGGAAGAGGAAAATGACTGTAGAGGGTGGGACAACCAAGAAAGCAAAATGTCCTGATGCAGTTTCTGCTTATTCAGTAGATGATAACTCCCATCAGCAGCAGAAGAGGTTTCTCAAGAACAAGCAAGCCAAATATATAGAGAGTGGTCCAACCAAGAAAGCAAAGTTTATTGAGACGGAAGATGCAGTGTCAGGTGATTCAATGGAAGATGATTTCTGTCAGCAAAATAGGACGACTCTTCGAAGCGAGCAAGCCAAGTATATTGAGGGAGATGATGATGTTTCAGATGATTCAATGGGAGTTGATTCTCATCAGCAGCAGAGGCGGATTGCTAAAAGTAAGCAAGCGAAGTATATTGCCAGAGATTTCTCTATGTTGTCAGATGATTCAGTTGGAGTTAATTCTGATCATCAGCAGAGGCGGGTTGCTGAAAGTAATGCCAGGGAGTTCTCTGCAGTTTCTGATGATTCGCTTGAGGATAATATCCATCAGCTGCATAGGAGGTCACTTAGACGCAACAAAGGTAAATGCATTGGGAGAGGAAATTTGACTTCACAAAATCTTCACGGGGTTAGTTCTCGACAGCAACAAAGAAGGACTTCTAAAAGCAAGCAGGCCAAAATTGTGGAGAGAGAAGATGCAGCTCTGGATGACACACCGGACGATAATGCTACACTGCAGCTTAAGAGTTTCCGAGGCAGGCAAATAAAACCAGAGACCGTTCAGCAAAAGAAACAGGAAACTCCTCGCCGTGTGAAGCAAGGCTCCCGCCGTCTTCAAGAAACCCAGCAAAAGACTCCCAGGGTCCAAAATAATCAATCTGAACAAAACACTGTTGATGTAAATGCTGAGGAACCAGAAGGTGGTCCTAGCACGCGACTTAGAAAAAGACCTCCAAAAGAGCAGCCAGAAACCAGTAGGAAAAAGGCAAAAGAGCAGCCAGAAACTGGAAGGAAAAAGGCAAGAGAGCAGCAACAAACTGGTAGGATAAAGGTAAACACTGCTTTAGCTGTGAAGACAAAGAATGCTTCAGCTCGGAAGGGAAAGAATGCTTCAGCAGTGAGGGAAGAGGAAGCAGAATTCTTGTGTGACATTGAGGGGTGCACCATGAGTTTTGGCACGAAACAAGAGCTAAATCTCCACAAGAAAAATGTGTGTCCAGTCAAGGGGTGTGGAAAGAAATTCTTCTCGCACAAGTATCTGGTGCAACATCGTCGAGTTCATGAGGATGACCGCCCCCTTCGGTGCCCTTGGAAGGGATGCAAGATGACATTCAAGTGGGCATGGGCTCGGACTGAGCACATTAGGGTACACACAGGTGCCCGTCCCTATGTCTGTGCAGAGCCTGGGTGTGGGCAGACCTTCCGGTTTGTGTCAGATTTCAGCCGTCATAAGCGGAAGACTGGGCATTCAGTAAAGAAAGGGAGAATGGAGCTCTCTGAATCGTCCTCCTCTGTTTCCCCAAATCTGGATTTGCATATCGGCAACAGCAACGAAACCGATCAGGTTCAGCTCAACCATTCCGACATAGGGTCTCAACAGTCCACCGCCGCCTGCCAGCAGCTGGACAATCACTCCGCTGGTGACGCGGCGGTTACTATTCCCGATCCCGATGTGGCTGATGTCGTGGACCACGCTGTCGTCGTTGGTGAGATTGAGAGATTGGATTTGAGAGGAGACGATGTTGATGCAGCGGTGGCGGCGGAGGTAGAGGAAGGAGGAGAGGTTGAGGCGGAGAAGAGTTCTAATGATGGTGGTGGTGGTGGTGGAGAAATCGAAAACGGAAATGAAAGTGAGAGACAGAGCGAGGAAGAAGAGAGCAATGAAGGAGAAAACAGGAGTGAGAATGGAGTTGATGGAGAGAAGAAGGGGGAGAATATAACAGAAGGAATTATGGGGTGGTTGGTATGTTATTCTGGAATAATGAATATTGCCAGCGTTTTTAAAGACAAGGTGAAGGAAAAGGACGAGTTGTCAGAGAAGCCAAGCCAGACAGAATGCAAGTATTACTTAAGGCCAGGGGGATGTAAGTATGGGAAAGCTTGCAGATACAACCATGGAAAAGGAAACCTTCAGTTGCTCCAGTTGTGGAACTTAACTTTCTGGGACTGCCAATTCGACAGGTTTAATCACCCTGATCCTACTGCTGCAGGAGGATCTGACCTTCCGTCTGGATTTGATAATGGTGGACCTGCATCATTACAAGGTGGATCGCAATCATCATCTTGGTCTGCACCAAGATCATTGAATGAGACTCCGCTTTATATGCCAATGATGATGCCACCATCTCAAGGGGTTCCTTCCCAAAATACAGAATGGAATGGCTATCAGGCACCAGTTTATCTAGAAAGAAGCATGCCTGCACCTCCACCATATGTTATCAACAACTCAGGGACTGAAACCAATGTTTATAAACAATATCCACTGTCAAATCAAGTTGATGAATTCCCAGAACGACCTGGACAACCTTTGTGCAATTTCTTTTTAAGAACAGGGGACTGTAAGTTTAAATCTAACTGCAAATATCACCATCCAAAAAGTCAGACTGCAGTATCCCCCTCATTTGCACTGAGTGACAAGGGCCTGCCTTTGAGACCAGATCAGAATATTTGCACCCATTACAGTCGCTATGGCATTTGCAAATTTGGGCCAGCTTGTAAATTTGATCATCCGATACATTTAACATCTTCAACTACATCTGGTCTTGATCAACTTCCTTTCAGTGACTTGGCAAATACAAAAGGGGCGGGAATAGCTAGGAGCAGAAGTGGAACTGATGATACAATTCAGCTGCAACAAGCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010605 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0099402 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

JmjC JmjN zf-C2H2

Protein Analysis

1930

Amino Acids

213.66

Weight (kDa)

7.79

Isoelectric Point (pI)

53.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjN	PF02375	21 - 54	2.6e-15	jmjN domain
JmjC	PF02373	208 - 297	1.3e-19	JmjC domain, hydroxylase
zf-CCCH	PF00642	1815 - 1841	1.9e-06	Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH	PF00642	1862 - 1886	5.1e-08	Zinc finger C-x8-C-x5-C-x3-H type (and similar)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0011462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48430 AT3G48430
fragaria_vesca	FvH4_5g07090 FvH4_5g07090 FvH4_5g07090
malus_domestica	MD06G1159300.v1.1 MD14G1165600.v1.1
prunus_persica	Prupe.5G159200_v2.0.a1
pyrus_communis	pycom06g14210 pycom14g13810
rosa_chinensis	RchiOBHm_Chr7g0191461
rosa_laevigata	RLG00000004448
rosa_multiflora	Rmu_sc0002661.1_g000009
rosa_roxburghii	Rroxscaffold_157G00438400 Rroxscaffold_3G00263790
rosa_rugosa	Rorug06G0510400
rosa_samantha	Rh7AG118500 Rh7BG120500 Rh7CG123300 Rh7DG121400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5397
AasI	GACNNNNNNGTC	1 cut(s)	4622
Acc36I	ACCTGC	3 cut(s)	786, 1273, 5185
AccB1I	GGYRCC	8 cut(s)	166, 175, 551, 2315, 2436, 4230, 4304, 5317
AccB7I	CCANNNNNTGG	3 cut(s)	3848, 3932, 4549
AccBSI	CCGCTC	2 cut(s)	342, 2658
AccII	CGCG	4 cut(s)	223, 2458, 3862, 4574
AccIII	TCCGGA	1 cut(s)	61
AcsI	RAATTY	8 cut(s)	818, 1473, 3529, 4064, 4166, 5429, 5627, 5645
AcuI	CTGAAG	5 cut(s)	783, 1173, 3999, 4023, 5042
AcyI	GRCGYC	2 cut(s)	176, 582
AdeI	CACNNNGTG	1 cut(s)	5558
AfeI	AGCGCT	1 cut(s)	1402
AflII	CTTAAG	2 cut(s)	3668, 4996
AflIII	ACRYGT	2 cut(s)	1216, 1466
AhdI	GACNNNNNGTC	4 cut(s)	2750, 4516, 4957, 5152
AjiI	CACGTC	1 cut(s)	2006
AleI	CACNNNNGTG	1 cut(s)	4302
Alw21I	GWGCWC	4 cut(s)	351, 4091, 4287, 4422
Alw44I	GTGCAC	1 cut(s)	4087
AlwNI	CAGNNNCTG	7 cut(s)	2747, 3050, 3446, 3932, 4331, 4549, 5381
Aor13HI	TCCGGA	1 cut(s)	61
Aor51HI	AGCGCT	1 cut(s)	1402
ApaLI	GTGCAC	1 cut(s)	4087
ApoI	RAATTY	8 cut(s)	818, 1473, 3529, 4064, 4166, 5429, 5627, 5645
ArsI	GACNNNNNNTTYG	4 cut(s)	2083, 2115, 5084, 5116
Asp700I	GAANNNNTTC	2 cut(s)	3583, 4169
AspLEI	GCGC	3 cut(s)	178, 223, 1403
AsuC2I	CCSGG	3 cut(s)	277, 304, 513
AsuII	TTCGAA	1 cut(s)	3226
AsuNHI	GCTAGC	1 cut(s)	2729
AvaII	GGWCC	7 cut(s)	1625, 2744, 3131, 3790, 3851, 4615, 5174
AxyI	CCTNAGG	1 cut(s)	2748
BaeGI	GKGCMC	6 cut(s)	648, 1686, 2320, 4091, 4235, 4309
BaeI	ACNNNNGTAYC	2 cut(s)	5520, 5553
BanI	GGYRCC	8 cut(s)	166, 175, 551, 2315, 2436, 4230, 4304, 5317
BanII	GRGCYC	3 cut(s)	49, 4276, 4422
BbsI	GAAGAC	5 cut(s)	635, 749, 3752, 4007, 4391
Bbv12I	GWGCWC	4 cut(s)	351, 4091, 4287, 4422
BbvCI	CCTCAGC	1 cut(s)	3834
BceAI	ACGGC	6 cut(s)	615, 644, 2358, 3720, 3741, 4356
BcgI	CGANNNNNNTGC	6 cut(s)	241, 275, 4181, 4215, 4655, 4689
BciVI	GTATCC	1 cut(s)	5548
BclI	TGATCA	3 cut(s)	3391, 5650, 5692
BcnI	CCSGG	3 cut(s)	277, 304, 513
BfoI	RGCGCY	2 cut(s)	179, 1404
BfrI	CTTAAG	2 cut(s)	3668, 4996
BfuAI	ACCTGC	3 cut(s)	786, 1273, 5185
BfuI	GTATCC	1 cut(s)	5548
BlpI	GCTNAGC	1 cut(s)	533
BmcAI	AGTACT	1 cut(s)	1375
Bme18I	GGWCC	7 cut(s)	1625, 2744, 3131, 3790, 3851, 4615, 5174
BmeRI	GACNNNNNGTC	4 cut(s)	2750, 4516, 4957, 5152
BmgBI	CACGTC	1 cut(s)	2006
BmrI	ACTGGG	3 cut(s)	2504, 2617, 4398
BmtI	GCTAGC	1 cut(s)	2733
BmuI	ACTGGG	3 cut(s)	2504, 2617, 4398
BpiI	GAAGAC	5 cut(s)	635, 749, 3752, 4007, 4391
BpmI	CTGGAG	3 cut(s)	1947, 2709, 5051
Bpu10I	CCTNAGC	2 cut(s)	2651, 3834
Bpu1102I	GCTNAGC	1 cut(s)	533
Bpu14I	TTCGAA	1 cut(s)	3226
BpuEI	CTTGAG	4 cut(s)	2291, 3083, 3479, 5259
BpuMI	CCSGG	3 cut(s)	277, 304, 513
BsaAI	YACGTR	1 cut(s)	1217
BsaBI	GATNNNNATC	3 cut(s)	2624, 3294, 5200
BsaHI	GRCGYC	2 cut(s)	176, 582
BsaI	GGTCTC	5 cut(s)	319, 443, 3695, 4524, 5572
BsaWI	WCCGGW	3 cut(s)	61, 3643, 4350
Bse118I	RCCGGY	2 cut(s)	163, 172
Bse21I	CCTNAGG	1 cut(s)	2748
Bse3DI	GCAATG	7 cut(s)	891, 1372, 1996, 2440, 2589, 2913, 4813
Bse8I	GATNNNNATC	3 cut(s)	2624, 3294, 5200
BseAI	TCCGGA	1 cut(s)	61
BseJI	GATNNNNATC	3 cut(s)	2624, 3294, 5200
BseMI	GCAATG	7 cut(s)	891, 1372, 1996, 2440, 2589, 2913, 4813
BseMII	CTCAG	9 cut(s)	55, 524, 894, 2739, 2946, 3825, 4272, 5388, 5546
BseSI	GKGCMC	6 cut(s)	648, 1686, 2320, 4091, 4235, 4309
BseYI	CCCAGC	4 cut(s)	589, 812, 2594, 3771
BsgI	GTGCAG	4 cut(s)	341, 4344, 5202, 5331
Bsh1236I	CGCG	4 cut(s)	223, 2458, 3862, 4574
BshNI	GGYRCC	8 cut(s)	166, 175, 551, 2315, 2436, 4230, 4304, 5317
BsiHKAI	GWGCWC	4 cut(s)	351, 4091, 4287, 4422
BsiSI	CCGG	8 cut(s)	62, 164, 173, 277, 304, 513, 3644, 4351
BslFI	GGGAC	6 cut(s)	1611, 3028, 4296, 5105, 5392, 5495
BsmBI	CGTCTC	3 cut(s)	590, 3152, 4656
BsmFI	GGGAC	6 cut(s)	1611, 3028, 4296, 5105, 5392, 5495
BsmI	GAATGC	5 cut(s)	2259, 4015, 4039, 4393, 4988
Bso31I	GGTCTC	5 cut(s)	319, 443, 3695, 4524, 5572
Bsp119I	TTCGAA	1 cut(s)	3226
Bsp13I	TCCGGA	1 cut(s)	61
Bsp1407I	TGTACA	1 cut(s)	1631
Bsp1720I	GCTNAGC	1 cut(s)	533
Bsp19I	CCATGG	1 cut(s)	5041
BspCNI	CTCAG	9 cut(s)	54, 525, 895, 2740, 2947, 3826, 4273, 5387, 5547
BspEI	TCCGGA	1 cut(s)	61
BspFNI	CGCG	4 cut(s)	223, 2458, 3862, 4574
BspHI	TCATGA	3 cut(s)	1254, 2857, 4207
BspMAI	CTGCAG	6 cut(s)	797, 2743, 3442, 3666, 5137, 5536
BspMI	ACCTGC	3 cut(s)	786, 1273, 5185
BspOI	GCTAGC	1 cut(s)	2733
BspQI	GCTCTTC	1 cut(s)	389
BspT104I	TTCGAA	1 cut(s)	3226
BspT107I	GGYRCC	8 cut(s)	166, 175, 551, 2315, 2436, 4230, 4304, 5317
BspTI	CTTAAG	2 cut(s)	3668, 4996
BspTNI	GGTCTC	5 cut(s)	319, 443, 3695, 4524, 5572
BsrBI	CCGCTC	2 cut(s)	342, 2658
BsrDI	GCAATG	7 cut(s)	891, 1372, 1996, 2440, 2589, 2913, 4813
BsrFI	RCCGGY	2 cut(s)	163, 172
BsrGI	TGTACA	1 cut(s)	1631
BssAI	RCCGGY	2 cut(s)	163, 172
BssNI	GRCGYC	2 cut(s)	176, 582
BssT1I	CCWWGG	2 cut(s)	4235, 5041
Bst6I	CTCTTC	9 cut(s)	389, 539, 2639, 2984, 3083, 3228, 4047, 4718, 4794
BstACI	GRCGYC	2 cut(s)	176, 582
BstAFI	CTTAAG	2 cut(s)	3668, 4996
BstAPI	GCANNNNNTGC	1 cut(s)	3050
BstAUI	TGTACA	1 cut(s)	1631
BstBAI	YACGTR	1 cut(s)	1217
BstBI	TTCGAA	1 cut(s)	3226
BstDSI	CCRYGG	3 cut(s)	660, 2456, 5041
BstFNI	CGCG	4 cut(s)	223, 2458, 3862, 4574
BstH2I	RGCGCY	2 cut(s)	179, 1404
BstHHI	GCGC	3 cut(s)	178, 223, 1403
BstNSI	RCATGY	5 cut(s)	511, 1470, 2261, 2279, 5344
BstSLI	GKGCMC	6 cut(s)	648, 1686, 2320, 4091, 4235, 4309
BstUI	CGCG	4 cut(s)	223, 2458, 3862, 4574
BstV2I	GAAGAC	5 cut(s)	635, 749, 3752, 4007, 4391
BstX2I	RGATCY	5 cut(s)	94, 1603, 1720, 2119, 5141
BstXI	CCANNNNNNTGG	1 cut(s)	3610
BstYI	RGATCY	5 cut(s)	94, 1603, 1720, 2119, 5141
Bsu36I	CCTNAGG	1 cut(s)	2748
BsuI	GTATCC	1 cut(s)	5548
BtgI	CCRYGG	3 cut(s)	660, 2456, 5041
BtrI	CACGTC	1 cut(s)	2006
BtsI	GCAGTG	4 cut(s)	3177, 3659, 3984, 4050
BveI	ACCTGC	3 cut(s)	786, 1273, 5185
CaiI	CAGNNNCTG	7 cut(s)	2747, 3050, 3446, 3932, 4331, 4549, 5381
CciI	TCATGA	3 cut(s)	1254, 2857, 4207
CfoI	GCGC	3 cut(s)	178, 223, 1403
Cfr10I	RCCGGY	2 cut(s)	163, 172
Cfr42I	CCGCGG	1 cut(s)	2459
CseI	GACGC	4 cut(s)	571, 739, 3507, 4580
CspCI	CAANNNNNGTGG	6 cut(s)	59, 94, 529, 564, 4138, 4173
DinI	GGCGCC	1 cut(s)	177
DraI	TTTAAA	2 cut(s)	4930, 5494
DraIII	CACNNNGTG	1 cut(s)	5558
DrdI	GACNNNNNNGTC	1 cut(s)	4622
DriI	GACNNNNNGTC	4 cut(s)	2750, 4516, 4957, 5152
DseDI	GACNNNNNNGTC	1 cut(s)	4622
Eam1104I	CTCTTC	9 cut(s)	389, 539, 2639, 2984, 3083, 3228, 4047, 4718, 4794
Eam1105I	GACNNNNNGTC	4 cut(s)	2750, 4516, 4957, 5152
EarI	CTCTTC	9 cut(s)	389, 539, 2639, 2984, 3083, 3228, 4047, 4718, 4794
EciI	GGCGGA	3 cut(s)	3325, 4703, 4733
Ecl136II	GAGCTC	1 cut(s)	4420
Eco130I	CCWWGG	2 cut(s)	4235, 5041
Eco147I	AGGCCT	2 cut(s)	429, 2767
Eco24I	GRGCYC	3 cut(s)	49, 4276, 4422
Eco31I	GGTCTC	5 cut(s)	319, 443, 3695, 4524, 5572
Eco32I	GATATC	2 cut(s)	445, 1529
Eco47I	GGWCC	7 cut(s)	1625, 2744, 3131, 3790, 3851, 4615, 5174
Eco47III	AGCGCT	1 cut(s)	1402
Eco53kI	GAGCTC	1 cut(s)	4420
Eco57I	CTGAAG	5 cut(s)	783, 1173, 3999, 4023, 5042
Eco81I	CCTNAGG	1 cut(s)	2748
EcoICRI	GAGCTC	1 cut(s)	4420
EcoO109I	RGGNCCY	3 cut(s)	1625, 2744, 5566
EcoRI	GAATTC	2 cut(s)	4064, 5429
EcoRV	GATATC	2 cut(s)	445, 1529
EcoT14I	CCWWGG	2 cut(s)	4235, 5041
EcoT22I	ATGCAT	3 cut(s)	2259, 2710, 3518
EcoT38I	GRGCYC	3 cut(s)	49, 4276, 4422
EgeI	GGCGCC	1 cut(s)	177
EheI	GGCGCC	1 cut(s)	177
ErhI	CCWWGG	2 cut(s)	4235, 5041
Esp3I	CGTCTC	3 cut(s)	590, 3152, 4656
FaqI	GGGAC	6 cut(s)	1611, 3028, 4296, 5105, 5392, 5495
FauI	CCCGC	5 cut(s)	320, 2098, 3399, 3761, 5725
FauNDI	CATATG	1 cut(s)	5359
FbaI	TGATCA	3 cut(s)	3391, 5650, 5692
FriOI	GRGCYC	3 cut(s)	49, 4276, 4422
GlaI	GCGC	3 cut(s)	177, 222, 1402
GsaI	CCCAGC	4 cut(s)	593, 816, 2598, 3775
GsuI	CTGGAG	3 cut(s)	1947, 2709, 5051
HaeII	RGCGCY	2 cut(s)	179, 1404
HapII	CCGG	8 cut(s)	62, 164, 173, 277, 304, 513, 3644, 4351
HgaI	GACGC	4 cut(s)	571, 739, 3507, 4580
HhaI	GCGC	3 cut(s)	178, 223, 1403
Hin1I	GRCGYC	2 cut(s)	176, 582
Hin6I	GCGC	3 cut(s)	176, 221, 1401
HinP1I	GCGC	3 cut(s)	176, 221, 1401
HindIII	AAGCTT	1 cut(s)	5025
HpaII	CCGG	8 cut(s)	62, 164, 173, 277, 304, 513, 3644, 4351
Hpy166II	GTNNAC	9 cut(s)	475, 1226, 1240, 3982, 4089, 4297, 4530, 4615, 5174
Hpy8I	GTNNAC	9 cut(s)	475, 1226, 1240, 3982, 4089, 4297, 4530, 4615, 5174
Hpy99I	CGWCG	4 cut(s)	733, 942, 4203, 4631
HpyCH4IV	ACGT	2 cut(s)	1216, 2005
HpySE526I	ACGT	2 cut(s)	1216, 2005
Hsp92I	GRCGYC	2 cut(s)	176, 582
HspAI	GCGC	3 cut(s)	176, 221, 1401
KasI	GGCGCC	1 cut(s)	175
KflI	GGGWCCC	1 cut(s)	1625
Kpn2I	TCCGGA	1 cut(s)	61
KroI	GCCGGC	1 cut(s)	172
KroNI	GCCGGC	1 cut(s)	174
Ksp22I	TGATCA	3 cut(s)	3391, 5650, 5692
KspI	CCGCGG	1 cut(s)	2459
LguI	GCTCTTC	1 cut(s)	389
LmnI	GCTCC	8 cut(s)	13, 64, 2150, 2304, 3755, 4417, 5070, 5745
MaeII	ACGT	2 cut(s)	1216, 2005
MbiI	CCGCTC	2 cut(s)	342, 2658
MfeI	CAATTG	2 cut(s)	192, 1164
MflI	RGATCY	5 cut(s)	94, 1603, 1720, 2119, 5141
Mly113I	GGCGCC	1 cut(s)	176
MlyI	GAGTC	5 cut(s)	1784, 1901, 3214, 3775, 5233
MmeI	TCCRAC	6 cut(s)	220, 961, 1964, 3158, 3358, 4533
Mph1103I	ATGCAT	3 cut(s)	2259, 2710, 3518
MreI	CGCCGGCG	1 cut(s)	172
MroI	TCCGGA	1 cut(s)	61
MroNI	GCCGGC	1 cut(s)	172
MroXI	GAANNNNTTC	2 cut(s)	3583, 4169
MslI	CAYNNNNRTG	5 cut(s)	752, 1324, 4212, 4261, 4302
MspA1I	CMGCKG	8 cut(s)	532, 1925, 2458, 3478, 4549, 4565, 4679, 5776
MspCI	CTTAAG	2 cut(s)	3668, 4996
MspI	CCGG	8 cut(s)	62, 164, 173, 277, 304, 513, 3644, 4351
MunI	CAATTG	2 cut(s)	192, 1164
Mva1269I	GAATGC	5 cut(s)	2259, 4015, 4039, 4393, 4988
MvnI	CGCG	4 cut(s)	223, 2458, 3862, 4574
NaeI	GCCGGC	1 cut(s)	174
NarI	GGCGCC	1 cut(s)	176
NciI	CCSGG	3 cut(s)	277, 304, 513
NcoI	CCATGG	1 cut(s)	5041
NdeI	CATATG	1 cut(s)	5359
NgoMIV	GCCGGC	1 cut(s)	172
NheI	GCTAGC	1 cut(s)	2729
NmeAIII	GCCGAG	2 cut(s)	109, 497
NsiI	ATGCAT	3 cut(s)	2259, 2710, 3518
NspI	RCATGY	5 cut(s)	511, 1470, 2261, 2279, 5344
NspV	TTCGAA	1 cut(s)	3226
OliI	CACNNNNGTG	1 cut(s)	4302
PaeI	GCATGC	2 cut(s)	2279, 5344
PagI	TCATGA	3 cut(s)	1254, 2857, 4207
PasI	CCCWGGG	2 cut(s)	648, 3786
PceI	AGGCCT	2 cut(s)	429, 2767
PciI	ACATGT	1 cut(s)	1466
PciSI	GCTCTTC	1 cut(s)	389
PcsI	WCGNNNNNNNCGW	3 cut(s)	1051, 1683, 4626
PctI	GAATGC	5 cut(s)	2259, 4015, 4039, 4393, 4988
PdiI	GCCGGC	1 cut(s)	174
PdmI	GAANNNNTTC	2 cut(s)	3583, 4169
PflMI	CCANNNNNTGG	3 cut(s)	3848, 3932, 4549
PfoI	TCCNGGA	1 cut(s)	2227
PleI	GAGTC	5 cut(s)	1784, 1901, 3214, 3775, 5233
PluTI	GGCGCC	1 cut(s)	179
PpsI	GAGTC	5 cut(s)	1784, 1901, 3214, 3775, 5233
Ppu21I	YACGTR	1 cut(s)	1217
PpuMI	RGGWCCY	2 cut(s)	1625, 2744
PscI	ACATGT	1 cut(s)	1466
PsiI	TTATAA	1 cut(s)	5397
Psp124BI	GAGCTC	1 cut(s)	4422
Psp5II	RGGWCCY	2 cut(s)	1625, 2744
PspFI	CCCAGC	4 cut(s)	589, 812, 2594, 3771
PspPPI	RGGWCCY	2 cut(s)	1625, 2744
PsrI	GAACNNNNNNTAC	2 cut(s)	5749, 5781
PstI	CTGCAG	6 cut(s)	797, 2743, 3442, 3666, 5137, 5536
PstNI	CAGNNNCTG	7 cut(s)	2747, 3050, 3446, 3932, 4331, 4549, 5381
PsuI	RGATCY	5 cut(s)	94, 1603, 1720, 2119, 5141
PvuII	CAGCTG	4 cut(s)	1925, 3478, 4549, 5776
RseI	CAYNNNNRTG	5 cut(s)	752, 1324, 4212, 4261, 4302
SacI	GAGCTC	1 cut(s)	4422
SacII	CCGCGG	1 cut(s)	2459
SapI	GCTCTTC	1 cut(s)	389
SbfI	CCTGCAGG	1 cut(s)	2743
ScaI	AGTACT	1 cut(s)	1375
SchI	GAGTC	5 cut(s)	1784, 1901, 3214, 3775, 5233
SdaI	CCTGCAGG	1 cut(s)	2743
SfoI	GGCGCC	1 cut(s)	177
Sfr303I	CCGCGG	1 cut(s)	2459
SfuI	TTCGAA	1 cut(s)	3226
SgrAI	CRCCGGYG	2 cut(s)	163, 172
SgrBI	CCGCGG	1 cut(s)	2459
SinI	GGWCC	7 cut(s)	1625, 2744, 3131, 3790, 3851, 4615, 5174
SmiMI	CAYNNNNRTG	5 cut(s)	752, 1324, 4212, 4261, 4302
SmlI	CTYRAG	6 cut(s)	2270, 3098, 3458, 3668, 4996, 5274
SmoI	CTYRAG	6 cut(s)	2270, 3098, 3458, 3668, 4996, 5274
SphI	GCATGC	2 cut(s)	2279, 5344
Sse8387I	CCTGCAGG	1 cut(s)	2743
SseBI	AGGCCT	2 cut(s)	429, 2767
SspDI	GGCGCC	1 cut(s)	175
SspI	AATATT	2 cut(s)	4915, 5593
SstI	GAGCTC	1 cut(s)	4422
StuI	AGGCCT	2 cut(s)	429, 2767
StyI	CCWWGG	2 cut(s)	4235, 5041
TaiI	ACGT	2 cut(s)	1219, 2008
TatI	WGTACW	4 cut(s)	1168, 1373, 1631, 2207
TauI	GCSGC	8 cut(s)	163, 172, 345, 532, 2658, 4538, 4577, 4688
TspGWI	ACGGA	7 cut(s)	478, 879, 2212, 2291, 3176, 4782, 5142
Van91I	CCANNNNNTGG	3 cut(s)	3848, 3932, 4549
Vha464I	CTTAAG	2 cut(s)	3668, 4996
VneI	GTGCAC	1 cut(s)	4087
VpaK11BI	GGWCC	7 cut(s)	1625, 2744, 3131, 3790, 3851, 4615, 5174
XapI	RAATTY	8 cut(s)	818, 1473, 3529, 4064, 4166, 5429, 5627, 5645
XbaI	TCTAGA	2 cut(s)	2249, 5329
XceI	RCATGY	5 cut(s)	511, 1470, 2261, 2279, 5344
XcmI	CCANNNNNNNNNTGG	2 cut(s)	630, 1595
XmnI	GAANNNNTTC	2 cut(s)	3583, 4169
ZrmI	AGTACT	1 cut(s)	1375
Zsp2I	ATGCAT	3 cut(s)	2259, 2710, 3518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.