MD06G1161900.v1.1

protein-L-isoaspartate O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008340
Physical Location & Seq
Forward (+)
30273904 .. 30276182
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1161900.v1.1.491

Sequence Viewer

Length: 942 bp
ATGCGCTGTCTTTTTCTATTCCCTGCTGCAGCGAGAGCAACAAGAAGTGCTTTAGGCCGCTTATCGTATGGTTCCCGCTATCCAGCGCCCGGTGTGCAACAAGTCTTAAACTTCACCACTCGCGCTCAGCGCCATAGTCGTCGTCACGCACTCGCTCTCCTCCCAACCACCGCTTTCTCCTCCTCGTCGTTCCCTCTCCTCCAAAACCCTAATTTCCTCACGGGTAACCGTAACTCTTTCTTCCGCGGAATGGAGCAATACTGGTCCGGGAGTAGCATTAATAAGAACAAGGGGATGGTGGATCATTTGAAGAGTTATGGAGTGATTAGATCCAAAAGGGTTGCTGAAGTGATGGAGACTATTGATAGGGCTTTATTTGTGCCGGACGGAACCCCACCTTATGTTGATTCCCCCATGGCAATAGGTTATAATGCCACCATTTCTGCACCTCATATGCATGCAATGTGCCTTCAATTGTTGGAAGAGCATTTGCAGCCTGGAATGCGCGCTTTGGATGTTGGCTCGGGGACGGGATACTTGACGGCGTGTTTTGCGTTGATGGTTGGGCCACAAGGTCGCACAGTTGGTGTCGAGCACATTCCTGAGTTGGTTTCTTCCTCAATTCTGAATATTGAAAAAAGTGCAGCAGGATCATTATTGAGAGAAGGTTCCCTCTCAGTGCATGTTGGTGACGGAAGGCTAGGTTGGCCAGAGTTTGCTCCTTACGATGCCATTCACGTCGGTGCAGCTGCACCAGAAATCCCACAACCACTTATTGACCAGCTGAAGCCTGGAGGCAGAATGGTGATTCCAGTGGGAAACATATTCCAGGATCTTAAGGTTGTGGACAAGGACTTGGATGGTTCGATCAGTGTCCGGGATGAAACTTCGGTTCGTTATGTTCCTCTCACAAACCGAGATGATCAGCTGCGAGGCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCMT

Protein Analysis

314

Amino Acids

34.09

Weight (kDa)

8.99

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCMT PF01135 95 - 306 8.8e-85 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 429
AccII CGCG 3 cut(s) 123, 246, 507
AciI CCGC 5 cut(s) 58, 76, 171, 244, 246
AclWI GGATC 4 cut(s) 309, 324, 658, 840
AcoI YGGCCR 1 cut(s) 707
AcuI CTGAAG 2 cut(s) 366, 806
AfiI CCNNNNNNNGG 2 cut(s) 89, 250
AflII CTTAAG 1 cut(s) 836
AgsI TTSAA 3 cut(s) 310, 473, 635
AjiI CACGTC 1 cut(s) 739
AjnI CCWGG 3 cut(s) 496, 790, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AleI CACNNNNGTG 1 cut(s) 741
AluBI AGCT 3 cut(s) 749, 784, 928
AluI AGCT 3 cut(s) 749, 784, 928
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 4 cut(s) 309, 324, 658, 840
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 3 cut(s) 55, 566, 707
ApeKI GCWGC 7 cut(s) 26, 29, 493, 644, 746, 749, 928
AseI ATTAAT 1 cut(s) 279
AspLEI GCGC 6 cut(s) 6, 88, 125, 132, 507, 509
AspS9I GGNCC 2 cut(s) 264, 566
AsuC2I CCSGG 3 cut(s) 90, 268, 878
AsuHPI GGTGA 3 cut(s) 106, 701, 817
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 1 cut(s) 264
BalI TGGCCA 1 cut(s) 709
BauI CACGAG 1 cut(s) 937
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 7 cut(s) 13, 41, 505, 656, 736, 758, 915
BccI CCATC 4 cut(s) 289, 346, 553, 854
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 3 cut(s) 498, 792, 830
BciVI GTATCC 1 cut(s) 527
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 3 cut(s) 90, 268, 878
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 1 cut(s) 27
BfoI RGCGCY 2 cut(s) 89, 133
BfrI CTTAAG 1 cut(s) 836
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 8 cut(s) 27, 30, 58, 494, 645, 747, 750, 929
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 8 cut(s) 28, 31, 59, 495, 646, 748, 751, 930
Bme1390I CCNGG 6 cut(s) 90, 268, 498, 792, 830, 878
Bme18I GGWCC 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 523
BmgBI CACGTC 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 264, 566
BmiI GGNNCC 3 cut(s) 73, 391, 670
BmrFI CCNGG 6 cut(s) 90, 268, 498, 792, 830, 878
BmsI GCATC 1 cut(s) 718
BpmI CTGGAG 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 126
BpuMI CCSGG 3 cut(s) 90, 268, 878
BsaJI CCNNGG 2 cut(s) 244, 414
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 250
Bse1I ACTGG 2 cut(s) 266, 812
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 3 cut(s) 498, 792, 830
BseDI CCNNGG 2 cut(s) 244, 414
BseGI GGATG 4 cut(s) 300, 520, 865, 886
BseLI CCNNNNNNNGG 2 cut(s) 89, 250
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 3 cut(s) 140, 594, 690
BseNI ACTGG 2 cut(s) 266, 812
BsePI GCGCGC 1 cut(s) 505
BseRI GAGGAG 4 cut(s) 149, 169, 172, 188
BseXI GCAGC 7 cut(s) 13, 41, 505, 656, 736, 758, 915
BsgI GTGCAG 4 cut(s) 429, 663, 735, 765
Bsh1236I CGCG 3 cut(s) 123, 246, 507
BshFI GGCC 3 cut(s) 57, 568, 709
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 4 cut(s) 90, 267, 383, 877
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 89, 250
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 541
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 3 cut(s) 57, 568, 709
BsoBI CYCGRG 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 6 cut(s) 301, 329, 650, 832, 867, 922
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 5 cut(s) 58, 76, 171, 244, 246
BspANI GGCC 3 cut(s) 57, 568, 709
BspCNI CTCAG 3 cut(s) 139, 595, 689
BspFNI CGCG 3 cut(s) 123, 246, 507
BspLI GGNNCC 3 cut(s) 73, 391, 670
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 4 cut(s) 309, 324, 658, 840
BspQI GCTCTTC 1 cut(s) 477
BspTI CTTAAG 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 2 cut(s) 266, 812
BssECI CCNNGG 2 cut(s) 244, 414
BssHII GCGCGC 1 cut(s) 505
BssMI GATC 6 cut(s) 301, 329, 650, 832, 867, 922
BssSI CACGAG 1 cut(s) 937
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 937
Bst2UI CCWGG 3 cut(s) 498, 792, 830
Bst4CI ACNGT 2 cut(s) 230, 583
Bst6I CTCTTC 2 cut(s) 305, 477
BstAFI CTTAAG 1 cut(s) 836
BstC8I GCNNGC 2 cut(s) 459, 507
BstDEI CTNAG 3 cut(s) 126, 603, 676
BstDSI CCRYGG 2 cut(s) 244, 414
BstEII GGTNACC 1 cut(s) 224
BstF5I GGATG 4 cut(s) 300, 520, 865, 886
BstFNI CGCG 3 cut(s) 123, 246, 507
BstH2I RGCGCY 2 cut(s) 89, 133
BstHHI GCGC 6 cut(s) 6, 88, 125, 132, 507, 509
BstKTI GATC 6 cut(s) 304, 332, 653, 835, 870, 925
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 6 cut(s) 301, 329, 650, 832, 867, 922
BstMWI GCNNNNNNNGC 7 cut(s) 35, 94, 129, 493, 502, 551, 706
BstNI CCWGG 3 cut(s) 498, 792, 830
BstNSI RCATGY 2 cut(s) 461, 686
BstPI GGTNACC 1 cut(s) 224
BstSCI CCNGG 6 cut(s) 88, 266, 496, 790, 828, 876
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 3 cut(s) 123, 246, 507
BstV1I GCAGC 7 cut(s) 13, 41, 505, 656, 736, 758, 915
BstX2I RGATCY 2 cut(s) 329, 832
BstYI RGATCY 2 cut(s) 329, 832
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 3 cut(s) 57, 568, 709
BtgI CCRYGG 2 cut(s) 244, 414
BtrI CACGTC 1 cut(s) 739
BtsCI GGATG 4 cut(s) 300, 520, 865, 886
BtsIMutI CAGTG 3 cut(s) 684, 819, 877
Cac8I GCNNGC 2 cut(s) 459, 507
CfoI GCGC 6 cut(s) 6, 88, 125, 132, 507, 509
Cfr13I GGNCC 2 cut(s) 264, 566
Cfr42I CCGCGG 1 cut(s) 247
CviAII CATG 3 cut(s) 415, 458, 683
DdeI CTNAG 3 cut(s) 126, 603, 676
DpnI GATC 6 cut(s) 303, 331, 652, 834, 869, 924
DpnII GATC 6 cut(s) 301, 329, 650, 832, 867, 922
EaeI YGGCCR 1 cut(s) 707
Eam1104I CTCTTC 2 cut(s) 305, 477
EarI CTCTTC 2 cut(s) 305, 477
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 264
Eco57I CTGAAG 2 cut(s) 366, 806
Eco88I CYCGRG 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 224
EcoO65I GGTNACC 1 cut(s) 224
EcoRII CCWGG 3 cut(s) 496, 790, 828
EcoT14I CCWWGG 1 cut(s) 414
EcoT22I ATGCAT 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 3 cut(s) 418, 461, 686
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 1 cut(s) 541
FatI CATG 3 cut(s) 414, 457, 682
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 453
FbaI TGATCA 1 cut(s) 922
Fnu4HI GCNGC 8 cut(s) 27, 30, 58, 494, 645, 747, 750, 929
FokI GGATG 4 cut(s) 307, 527, 872, 893
Fsp4HI GCNGC 8 cut(s) 27, 30, 58, 494, 645, 747, 750, 929
FspBI CTAG 1 cut(s) 701
GlaI GCGC 6 cut(s) 5, 87, 124, 131, 506, 508
GluI GCNGC 8 cut(s) 27, 30, 58, 494, 645, 747, 750, 929
GsuI CTGGAG 1 cut(s) 813
HaeII RGCGCY 2 cut(s) 89, 133
HaeIII GGCC 3 cut(s) 57, 568, 709
HapII CCGG 4 cut(s) 90, 267, 383, 877
HhaI GCGC 6 cut(s) 6, 88, 125, 132, 507, 509
Hin1II CATG 3 cut(s) 418, 461, 686
Hin6I GCGC 6 cut(s) 4, 86, 123, 130, 505, 507
HinP1I GCGC 6 cut(s) 4, 86, 123, 130, 505, 507
HinfI GANTC 2 cut(s) 407, 808
HpaII CCGG 4 cut(s) 90, 267, 383, 877
HphI GGTGA 3 cut(s) 106, 701, 817
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 1 cut(s) 627
Hpy188III TCNNGA 2 cut(s) 602, 939
Hpy8I GTNNAC 1 cut(s) 847
Hpy99I CGWCG 3 cut(s) 144, 190, 743
HpyAV CCTTC 3 cut(s) 479, 659, 690
HpyCH4III ACNGT 2 cut(s) 230, 583
HpyCH4IV ACGT 1 cut(s) 738
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 94, 129, 493, 502, 551, 706
HpyF3I CTNAG 3 cut(s) 126, 603, 676
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 3 cut(s) 418, 461, 686
HspAI GCGC 6 cut(s) 4, 86, 123, 130, 505, 507
Ksp22I TGATCA 1 cut(s) 922
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 6 cut(s) 301, 329, 650, 832, 867, 922
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 2 cut(s) 253, 724
Lsp1109I GCAGC 7 cut(s) 13, 41, 505, 656, 736, 758, 915
LweI GCATC 1 cut(s) 718
MaeI CTAG 1 cut(s) 701
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 4 cut(s) 143, 224, 230, 689
MalI GATC 6 cut(s) 303, 331, 652, 834, 869, 924
MboI GATC 6 cut(s) 301, 329, 650, 832, 867, 922
MboII GAAGA 4 cut(s) 232, 322, 494, 606
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 2 cut(s) 329, 832
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 709
MluCI AATT 3 cut(s) 211, 473, 621
MluNI TGGCCA 1 cut(s) 709
MmeI TCCRAC 1 cut(s) 459
Mox20I TGGCCA 1 cut(s) 709
Mph1103I ATGCAT 1 cut(s) 459
MscI TGGCCA 1 cut(s) 709
MseI TTAA 3 cut(s) 107, 279, 837
MslI CAYNNNNRTG 3 cut(s) 456, 687, 741
Msp20I TGGCCA 1 cut(s) 709
MspA1I CMGCKG 4 cut(s) 246, 749, 784, 928
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 4 cut(s) 90, 267, 383, 877
MspR9I CCNGG 6 cut(s) 90, 268, 498, 792, 830, 878
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 3 cut(s) 498, 792, 830
MvnI CGCG 3 cut(s) 123, 246, 507
MwoI GCNNNNNNNGC 7 cut(s) 35, 94, 129, 493, 502, 551, 706
NciI CCSGG 3 cut(s) 90, 268, 878
NcoI CCATGG 1 cut(s) 414
NdeI CATATG 1 cut(s) 453
NdeII GATC 6 cut(s) 301, 329, 650, 832, 867, 922
NlaIII CATG 3 cut(s) 418, 461, 686
NlaIV GGNNCC 3 cut(s) 73, 391, 670
NmuCI GTSAC 2 cut(s) 143, 689
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 2 cut(s) 461, 686
OliI CACNNNNGTG 1 cut(s) 741
PaeI GCATGC 1 cut(s) 461
PauI GCGCGC 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 477
PctI GAATGC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 407, 808
PfoI TCCNGGA 3 cut(s) 266, 828, 876
PkrI GCNGC 8 cut(s) 28, 31, 59, 495, 646, 748, 751, 930
PshBI ATTAAT 1 cut(s) 279
PsiI TTATAA 1 cut(s) 429
Psp6I CCWGG 3 cut(s) 496, 790, 828
PspEI GGTNACC 1 cut(s) 224
PspGI CCWGG 3 cut(s) 496, 790, 828
PspN4I GGNNCC 3 cut(s) 73, 391, 670
PspPI GGNCC 2 cut(s) 264, 566
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 2 cut(s) 329, 832
PteI GCGCGC 1 cut(s) 505
PvuII CAGCTG 3 cut(s) 749, 784, 928
RseI CAYNNNNRTG 3 cut(s) 456, 687, 741
SacII CCGCGG 1 cut(s) 247
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 3 cut(s) 107, 279, 837
SatI GCNGC 8 cut(s) 27, 30, 58, 494, 645, 747, 750, 929
Sau3AI GATC 6 cut(s) 301, 329, 650, 832, 867, 922
Sau96I GGNCC 2 cut(s) 264, 566
ScrFI CCNGG 6 cut(s) 90, 268, 498, 792, 830, 878
SduI GDGCHC 1 cut(s) 597
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 247
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 1 cut(s) 264
SmiMI CAYNNNNRTG 3 cut(s) 456, 687, 741
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
SphI GCATGC 1 cut(s) 461
Sse9I AATT 3 cut(s) 211, 473, 621
SsiI CCGC 5 cut(s) 58, 76, 171, 244, 246
SspI AATATT 1 cut(s) 631
SspMI CTAG 1 cut(s) 701
StyD4I CCNGG 6 cut(s) 88, 266, 496, 790, 828, 876
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 230, 583
TaiI ACGT 1 cut(s) 741
TaqI TCGA 2 cut(s) 591, 866
TasI AATT 3 cut(s) 211, 473, 621
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 2 cut(s) 407, 808
Tru1I TTAA 3 cut(s) 107, 279, 837
Tru9I TTAA 3 cut(s) 107, 279, 837
TscAI CASTG 3 cut(s) 684, 819, 877
TseFI GTSAC 2 cut(s) 143, 689
TseI GCWGC 7 cut(s) 26, 29, 493, 644, 746, 749, 928
Tsp45I GTSAC 2 cut(s) 143, 689
TspDTI ATGAA 1 cut(s) 897
TspGWI ACGGA 2 cut(s) 402, 708
TspRI CASTG 3 cut(s) 684, 819, 877
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 1 cut(s) 264
VspI ATTAAT 1 cut(s) 279
XceI RCATGY 2 cut(s) 461, 686
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XspI CTAG 1 cut(s) 701
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.