Prupe.5G162200_v2.0.a1

protein-L-isoaspartate O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0008340
Physical Location & Seq
Forward (+)
14222758 .. 14225925
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G162200.1

Sequence Viewer

Length: 948 bp
ATGCATAAGCTACTCTCCCCAGCGAGAGCAACAAGTGCTATAGTAGGCCTAGACCGGTTAGCGTATGGTTCCCGCTATCCAGCGCCCACTGTGCAACACCTCTTAACCTTTACCACTCTCTCTTTCTTTCCTCACTCGCATCATCGTCATCGCCATCGTCCTCGCACACTCACACTTCTCCCCACCACCTCTCTCTCCTTCTGTCCTCTCCTCCAAAACCCTAATTTCCTCACGGGTAACTCCCTCTTCCGCGGAATGGAGCAATTCTGGTCCCATAGTAGCGTTAAGAAGAACAAGGCGATGGTGGAGAATTTGCAGAGTTATGGAGTGATTAGCTCCAAAAAGGTTGTTGAAGTGATGGGGACTATTGATAGAGCTTTATTTGTGCCTGATGGGACCCCACCTTATGTTGACTCCCCCATGGCAATAGGTTATAATGCCACCATTTCTGCACCTCATATGCATGCAACATGCCTTCAGTTGTTGGAAGAGAATTTGCAGCCTAATATGCGTGCTTTGGATATTGGTTCAGGGACGGGATATTTGACAGCTTGTTTTGCCTTGATGGTTGGGCCACAAGGCCGTGCAGTTGGTGTAGAGCACATTCCTGAGTTGGCTTCTTCCTCAATCGAGAATATTAAAAAAAGTGCAGCAGCACCATTATTGAAAGAAGGTTCTCTCTCAGTGCATGTCGGTGATGGAAGGCTAGGTTGGCCAGAATTTGCACCTTATGATGCCATTCATGTTGGTGCAGCTGCACCAGAAATCCCACAACCGCTTATTGACCAGTTGAAGCCTGGAGGCAGAATGGTGATTCCAGTGGGAAACATATTCCAGGATTTGAAGGTTGTGGACAAGAACTTGGATGGTTCTATCAGTGTCCGGACTGAAACTTCAGTTCGTTATGTTCCTCTCACAAGCAAAGATGCTCAGTTGCGAGGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCMT

Protein Analysis

316

Amino Acids

34.22

Weight (kDa)

9.1

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
AccII CGCG 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 882
AciI CCGC 4 cut(s) 73, 250, 252, 776
AcoI YGGCCR 1 cut(s) 713
AcsI RAATTY 3 cut(s) 310, 493, 719
AcuI CTGAAG 2 cut(s) 461, 879
AfiI CCNNNNNNNGG 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 353, 667, 793, 844
AjnI CCWGG 2 cut(s) 796, 834
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 5 cut(s) 10, 336, 377, 551, 755
AluI AGCT 5 cut(s) 10, 336, 377, 551, 755
Alw21I GWGCWC 1 cut(s) 603
Aor13HI TCCGGA 1 cut(s) 882
AoxI GGCC 4 cut(s) 46, 572, 580, 713
ApeKI GCWGC 5 cut(s) 499, 650, 653, 752, 755
ApoI RAATTY 3 cut(s) 310, 493, 719
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AsiGI ACCGGT 1 cut(s) 54
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 3 cut(s) 270, 396, 572
AsuHPI GGTGA 2 cut(s) 707, 823
AvaII GGWCC 2 cut(s) 270, 396
BalI TGGCCA 1 cut(s) 715
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 5 cut(s) 511, 662, 665, 742, 764
BccI CCATC 7 cut(s) 162, 295, 352, 386, 559, 692, 860
BceAI ACGGC 1 cut(s) 567
BciT130I CCWGG 2 cut(s) 798, 836
BfaI CTAG 2 cut(s) 50, 707
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 5 cut(s) 500, 651, 654, 753, 756
BlsI GCNGC 5 cut(s) 501, 652, 655, 754, 757
Bme1390I CCNGG 2 cut(s) 798, 836
Bme18I GGWCC 2 cut(s) 270, 396
BmgT120I GGNCC 3 cut(s) 270, 396, 572
BmiI GGNNCC 4 cut(s) 70, 272, 397, 398
BmrFI CCNGG 2 cut(s) 798, 836
BmsI GCATC 3 cut(s) 148, 724, 916
BpmI CTGGAG 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 250, 420
BsaWI WCCGGW 2 cut(s) 54, 882
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 787, 818
BseAI TCCGGA 1 cut(s) 882
BseBI CCWGG 2 cut(s) 798, 836
BseDI CCNNGG 2 cut(s) 250, 420
BseGI GGATG 1 cut(s) 871
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 3 cut(s) 600, 696, 944
BseNI ACTGG 2 cut(s) 787, 818
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 5 cut(s) 511, 662, 665, 742, 764
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 5 cut(s) 435, 606, 669, 741, 771
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 4 cut(s) 48, 574, 582, 715
BshTI ACCGGT 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 2 cut(s) 55, 883
BslFI GGGAC 4 cut(s) 256, 376, 409, 547
BslI CCNNNNNNNGG 1 cut(s) 256
BsmFI GGGAC 4 cut(s) 256, 376, 409, 547
BsnI GGCC 4 cut(s) 48, 574, 582, 715
Bsp1286I GDGCHC 1 cut(s) 603
Bsp13I TCCGGA 1 cut(s) 882
Bsp19I CCATGG 1 cut(s) 420
BspACI CCGC 4 cut(s) 73, 250, 252, 776
BspANI GGCC 4 cut(s) 48, 574, 582, 715
BspCNI CTCAG 3 cut(s) 601, 695, 943
BspEI TCCGGA 1 cut(s) 882
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 70, 272, 397, 398
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 2 cut(s) 787, 818
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 2 cut(s) 250, 420
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 798, 836
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 2 cut(s) 251, 483
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 465, 513
BstDEI CTNAG 3 cut(s) 609, 682, 930
BstDSI CCRYGG 2 cut(s) 250, 420
BstF5I GGATG 1 cut(s) 871
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstMWI GCNNNNNNNGC 5 cut(s) 35, 91, 508, 557, 712
BstNI CCWGG 2 cut(s) 798, 836
BstNSI RCATGY 3 cut(s) 467, 474, 692
BstSCI CCNGG 2 cut(s) 796, 834
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 5 cut(s) 511, 662, 665, 742, 764
BsuRI GGCC 4 cut(s) 48, 574, 582, 715
BtgI CCRYGG 2 cut(s) 250, 420
BtgZI GCGATG 2 cut(s) 134, 314
BtsCI GGATG 1 cut(s) 871
BtsIMutI CAGTG 4 cut(s) 87, 690, 825, 883
Cac8I GCNNGC 2 cut(s) 465, 513
CfoI GCGC 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 3 cut(s) 270, 396, 572
Cfr42I CCGCGG 1 cut(s) 253
CspAI ACCGGT 1 cut(s) 54
CviAII CATG 5 cut(s) 421, 464, 471, 689, 743
DdeI CTNAG 3 cut(s) 609, 682, 930
EaeI YGGCCR 1 cut(s) 713
Eam1104I CTCTTC 2 cut(s) 251, 483
EarI CTCTTC 2 cut(s) 251, 483
Eco130I CCWWGG 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 270, 396
Eco57I CTGAAG 2 cut(s) 461, 879
EcoO109I RGGNCCY 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 796, 834
EcoT14I CCWWGG 1 cut(s) 420
EcoT22I ATGCAT 2 cut(s) 6, 465
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 5 cut(s) 424, 467, 474, 692, 746
FaqI GGGAC 4 cut(s) 256, 376, 409, 547
FatI CATG 5 cut(s) 420, 463, 470, 688, 742
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 459
Fnu4HI GCNGC 5 cut(s) 500, 651, 654, 753, 756
FokI GGATG 1 cut(s) 878
Fsp4HI GCNGC 5 cut(s) 500, 651, 654, 753, 756
FspBI CTAG 2 cut(s) 50, 707
GlaI GCGC 1 cut(s) 84
GluI GCNGC 5 cut(s) 500, 651, 654, 753, 756
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 4 cut(s) 48, 574, 582, 715
HapII CCGG 2 cut(s) 55, 883
HhaI GCGC 1 cut(s) 85
Hin1II CATG 5 cut(s) 424, 467, 474, 692, 746
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 2 cut(s) 413, 814
HpaII CCGG 2 cut(s) 55, 883
HphI GGTGA 2 cut(s) 707, 823
Hpy166II GTNNAC 2 cut(s) 412, 853
Hpy188III TCNNGA 4 cut(s) 608, 631, 883, 945
Hpy8I GTNNAC 2 cut(s) 412, 853
HpyAV CCTTC 5 cut(s) 208, 485, 665, 696, 838
HpyCH4III ACNGT 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 91, 508, 557, 712
HpyF3I CTNAG 3 cut(s) 609, 682, 930
Hsp92II CATG 5 cut(s) 424, 467, 474, 692, 746
HspAI GCGC 1 cut(s) 83
KflI GGGWCCC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 882
KspI CCGCGG 1 cut(s) 253
LmnI GCTCC 2 cut(s) 259, 341
Lsp1109I GCAGC 5 cut(s) 511, 662, 665, 742, 764
LweI GCATC 3 cut(s) 148, 724, 916
MaeI CTAG 2 cut(s) 50, 707
MaeIII GTNAC 1 cut(s) 236
MboII GAAGA 4 cut(s) 238, 301, 500, 612
MhlI GDGCHC 1 cut(s) 603
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 5 cut(s) 223, 263, 310, 493, 719
MluNI TGGCCA 1 cut(s) 715
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 465
Mox20I TGGCCA 1 cut(s) 715
Mph1103I ATGCAT 2 cut(s) 6, 465
MroI TCCGGA 1 cut(s) 882
MscI TGGCCA 1 cut(s) 715
MseI TTAA 3 cut(s) 104, 285, 639
MslI CAYNNNNRTG 3 cut(s) 462, 693, 747
Msp20I TGGCCA 1 cut(s) 715
MspA1I CMGCKG 2 cut(s) 252, 755
MspI CCGG 2 cut(s) 55, 883
MspR9I CCNGG 2 cut(s) 798, 836
MvaI CCWGG 2 cut(s) 798, 836
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 5 cut(s) 35, 91, 508, 557, 712
NcoI CCATGG 1 cut(s) 420
NdeI CATATG 1 cut(s) 459
NlaIII CATG 5 cut(s) 424, 467, 474, 692, 746
NlaIV GGNNCC 4 cut(s) 70, 272, 397, 398
NsiI ATGCAT 2 cut(s) 6, 465
NspI RCATGY 3 cut(s) 467, 474, 692
PaeI GCATGC 1 cut(s) 467
PceI AGGCCT 1 cut(s) 48
PfeI GAWTC 1 cut(s) 814
PfoI TCCNGGA 1 cut(s) 834
PinAI ACCGGT 1 cut(s) 54
PkrI GCNGC 5 cut(s) 501, 652, 655, 754, 757
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PpuMI RGGWCCY 1 cut(s) 396
PsiI TTATAA 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 796, 834
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 2 cut(s) 796, 834
PspN4I GGNNCC 4 cut(s) 70, 272, 397, 398
PspPI GGNCC 3 cut(s) 270, 396, 572
PspPPI RGGWCCY 1 cut(s) 396
PvuII CAGCTG 1 cut(s) 755
RseI CAYNNNNRTG 3 cut(s) 462, 693, 747
SacII CCGCGG 1 cut(s) 253
SaqAI TTAA 3 cut(s) 104, 285, 639
SatI GCNGC 5 cut(s) 500, 651, 654, 753, 756
Sau96I GGNCC 3 cut(s) 270, 396, 572
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 798, 836
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 3 cut(s) 148, 724, 916
SfcI CTRYAG 1 cut(s) 39
Sfr303I CCGCGG 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 253
SinI GGWCC 2 cut(s) 270, 396
SmiMI CAYNNNNRTG 3 cut(s) 462, 693, 747
SphI GCATGC 1 cut(s) 467
Sse9I AATT 5 cut(s) 223, 263, 310, 493, 719
SseBI AGGCCT 1 cut(s) 48
SsiI CCGC 4 cut(s) 73, 250, 252, 776
SspI AATATT 1 cut(s) 637
SspMI CTAG 2 cut(s) 50, 707
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 796, 834
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 630
TasI AATT 5 cut(s) 223, 263, 310, 493, 719
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 3 cut(s) 104, 285, 639
Tru9I TTAA 3 cut(s) 104, 285, 639
TscAI CASTG 4 cut(s) 94, 690, 825, 883
TseI GCWGC 5 cut(s) 499, 650, 653, 752, 755
TspDTI ATGAA 1 cut(s) 731
TspRI CASTG 4 cut(s) 94, 690, 825, 883
VpaK11BI GGWCC 2 cut(s) 270, 396
XapI RAATTY 3 cut(s) 310, 493, 719
XceI RCATGY 3 cut(s) 467, 474, 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XspI CTAG 2 cut(s) 50, 707
Zsp2I ATGCAT 2 cut(s) 6, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.