Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004416

Physical Location & Seq

Genomic Coordinates Forward (+)

30549541 .. 30551108

Gene Structure 1568 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1165400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGCAATTTCCACAGCAGCAGCTGCAGCTGCAACCTCAGCTTCCTCAAAGCTCGCATACCCATATGCCTCCACAGCCTCCCCGTCCCCCACAACCTCTTCTTTCTCCATTAGACCTTCTTCCAAGCTCACCCACCTCTCCTCCTCCTTCCTCAACCCCTCCACCATCCTCCACCTCACACCCGCCTCCAACACCCGCCACCACCGCCGCAACTTCACCGTCAAAGCCGCCAGAGGTAAGTTCGAGCGCAAGAAGCCCCATGTCAACATCGGCACAATCGGCCACGTCGACCACGGCAAGACCACCCTCACCGCAGCCCTCACCATGGCCTTGGCCTCCATCGGCAATAGCGTCTCCAAGAAATACGACGAAATCGATGCCGCCCCGGAAGAGCGCGCCCGTGGAATCACCATCAACACCGCCACCGTCGAGTACGAGACCGAGAACCGCCACTACGCCCACGTCGACTGCCCCGGCCACGCCGATTACGTCAAGAACATGATTACCGGCGCCGCCCAGATGGACGGCGCCATTCTCGTCGTCTCTGGAGCCGACGGCCCAATGCCGCAGACCAAAGAGCACATCTTGCTGGCGAAGCAGGTGGGTGTGCCGAACATGGTGGTTTTCCTGAACAAGCAGGACCAGGTGGACGACGAGGAGCTCTTGCAGCTCGTCGAGTTGGAGGTGAGAGAGTTGCTTTCTTCCTATGAATTTCCTGGTGATGATGTGCCTATTATTTCTGGATCTGCACTGCTTGCACTAGAGGCTCTAATGGCGAATCCCACCATTTCGCGAGGCGAAAATCAATGGGTTGATAAGATTTATGAACTTATGGACTCTGTTGATGATTACATTCCGATTCCTCAGAGGCAGACTGAGCTGCCATTTTTGATGGCGATCGAAGATGTGTTTTCGATCACTGGCCGTGGCACTGTGGCCACTGGCCGTGTCGAGAGGGGGAAGGTTAAGGTCGGAGACACCGTCGACATTGTTGGCTTGAAGGACACCAGGAACACAACTGTCACCGGTGTCGAAATGTTTCAGAAAATTCTTGATGATGCCATGGCTGGTGACAATGTGGGGTTGTTGCTTAGGGGTATCCAGAAGATTGATATCCAGAGAGGGATGGTGTTGGCCAAACCCGGGACTATTACCCCGCATACCAAATTCGAGGCGATTGTGTATGTGTTGAAGAAGGAAGAGGGGGGAAGGCATTCACCGTTCTTTTCGGGTTACAGGCCGCAGTTTTACATGAGGACTACTGATGTCACCGGGAAGGTGACTTCGATTATGAACGATAAGGATGAGGAGTCGAAGATGGTGATGCCTGGCGATCGCGTTAAGATTGTGGTCGAGCTGATTGTTCCGGTGGCGTGCGAGCAGGGGATGAGGTTTGCCATCAGAGAAGGAGGGAAGACTGTTGGAGCTGGTGTTATTCAATCCATTATTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0009295 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031981 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0055035 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

485

Amino Acids

52.61

Weight (kDa)

6.15

Isoelectric Point (pI)

33.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	85 - 286	5.9e-63	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	310 - 379	1.3e-19	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	383 - 482	8.8e-40	Elongation factor Tu C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20360 AT4G20360
fragaria_vesca	FvH4_5g06350 FvH4_6g51880
malus_domestica	MD06G1165400.v1.1 MD09G1022300.v1.1 MD14G1170900.v1.1
prunus_persica	Prupe.3G294300_v2.0.a1 Prupe.5G165000_v2.0.a1 Prupe.5G165000_v2.0.a1
pyrus_communis	pycom06g14780 pycom111g01870 pycom14g14280
rosa_chinensis	RchiOBHm_Chr2g0173371 RchiOBHm_Chr7g0192491
rosa_laevigata	RLG00000004361 RLG00000022183
rosa_multiflora	Rmu_sc0003108.1_g000029 Rmu_sc0014554.1_g000001
rosa_roxburghii	Rroxscaffold_2G00078950 Rroxscaffold_3G00262980
rosa_rugosa	Rorug02G0567600 Rorug07G0001900
rosa_samantha	Rh2AG648100 Rh2BG658700 Rh2DG674100 Rh7AG125900 Rh7BG128200 Rh7CG129800 Rh7DG129700
rosa_wichuraiana	Rw2G053200 Rw7G010840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	589
Acc36I	ACCTGC	1 cut(s)	589
AccB1I	GGYRCC	2 cut(s)	509, 527
AccI	GTMKAC	3 cut(s)	288, 465, 984
AccII	CGCG	3 cut(s)	396, 793, 1338
AclWI	GGATC	1 cut(s)	751
AcoI	YGGCCR	6 cut(s)	280, 475, 922, 936, 943, 1134
AcsI	RAATTY	3 cut(s)	710, 1047, 1166
AcyI	GRCGYC	2 cut(s)	510, 528
AfaI	GTAC	1 cut(s)	434
AfiI	CCNNNNNNNGG	2 cut(s)	325, 1143
AgeI	ACCGGT	1 cut(s)	1025
AgsI	TTSAA	3 cut(s)	1000, 1192, 1439
AjiI	CACGTC	2 cut(s)	286, 463
AjnI	CCWGG	4 cut(s)	642, 715, 1007, 1327
Alw21I	GWGCWC	2 cut(s)	582, 663
Alw26I	GTCTC	4 cut(s)	358, 431, 547, 969
AlwI	GGATC	1 cut(s)	751
AlwNI	CAGNNNCTG	1 cut(s)	23
Ama87I	CYCGRG	1 cut(s)	1142
ApeKI	GCWGC	8 cut(s)	17, 20, 23, 26, 29, 314, 667, 880
ApoI	RAATTY	3 cut(s)	710, 1047, 1166
AsiGI	ACCGGT	1 cut(s)	1025
AsiSI	GCGATCGC	1 cut(s)	1336
Asp700I	GAANNNNTTC	2 cut(s)	1038, 1213
AspLEI	GCGC	5 cut(s)	249, 396, 398, 512, 530
AspS9I	GGNCC	2 cut(s)	557, 640
AsuC2I	CCSGG	5 cut(s)	386, 474, 1143, 1144, 1273
AvaI	CYCGRG	1 cut(s)	1142
AvaII	GGWCC	1 cut(s)	640
BalI	TGGCCA	2 cut(s)	938, 1136
BanI	GGYRCC	2 cut(s)	509, 527
BanII	GRGCYC	1 cut(s)	663
BbsI	GAAGAC	1 cut(s)	1421
Bbv12I	GWGCWC	2 cut(s)	582, 663
BbvCI	CCTCAGC	1 cut(s)	37
BbvI	GCAGC	8 cut(s)	10, 16, 29, 32, 38, 326, 679, 867
BccI	CCATC	8 cut(s)	173, 347, 419, 514, 886, 1120, 1312, 1406
BceAI	ACGGC	5 cut(s)	310, 541, 571, 909, 930
BcgI	CGANNNNNNTGC	2 cut(s)	359, 393
BciT130I	CCWGG	4 cut(s)	644, 717, 1009, 1329
BciVI	GTATCC	1 cut(s)	1109
BcnI	CCSGG	5 cut(s)	386, 474, 1143, 1144, 1273
BcoDI	GTCTC	4 cut(s)	358, 431, 547, 969
BfaI	CTAG	1 cut(s)	761
BfmI	CTRYAG	1 cut(s)	24
BfoI	RGCGCY	2 cut(s)	513, 531
BfuAI	ACCTGC	1 cut(s)	589
BfuI	GTATCC	1 cut(s)	1109
Bme1390I	CCNGG	9 cut(s)	386, 474, 644, 717, 1009, 1143, 1144, 1273, 1329
Bme18I	GGWCC	1 cut(s)	640
BmeT110I	CYCGRG	1 cut(s)	1142
BmgBI	CACGTC	2 cut(s)	286, 463
BmgT120I	GGNCC	2 cut(s)	557, 640
BmiI	GGNNCC	3 cut(s)	511, 529, 550
BmrFI	CCNGG	9 cut(s)	386, 474, 644, 717, 1009, 1143, 1144, 1273, 1329
BmsI	GCATC	3 cut(s)	367, 1048, 1314
BpiI	GAAGAC	1 cut(s)	1421
BpmI	CTGGAG	1 cut(s)	567
Bpu10I	CCTNAGC	2 cut(s)	37, 1091
BpuMI	CCSGG	5 cut(s)	386, 474, 1143, 1144, 1273
Bsa29I	ATCGAT	1 cut(s)	375
BsaBI	GATNNNNATC	2 cut(s)	896, 1112
BsaHI	GRCGYC	2 cut(s)	510, 528
BsaI	GGTCTC	1 cut(s)	431
BsaJI	CCNNGG	9 cut(s)	292, 324, 330, 384, 400, 472, 925, 1062, 1142
BsaWI	WCCGGW	2 cut(s)	1025, 1366
BsaXI	ACNNNNNCTCC	6 cut(s)	125, 155, 966, 996, 1416, 1446
Bsc4I	CCNNNNNNNGG	2 cut(s)	325, 1143
Bse118I	RCCGGY	2 cut(s)	506, 1025
Bse1I	ACTGG	2 cut(s)	925, 946
Bse8I	GATNNNNATC	2 cut(s)	896, 1112
BseBI	CCWGG	4 cut(s)	644, 717, 1009, 1329
BseCI	ATCGAT	1 cut(s)	375
BseDI	CCNNGG	9 cut(s)	292, 324, 330, 384, 400, 472, 925, 1062, 1142
BseGI	GGATG	4 cut(s)	165, 1131, 1309, 1392
BseJI	GATNNNNATC	2 cut(s)	896, 1112
BseLI	CCNNNNNNNGG	2 cut(s)	325, 1143
BseMII	CTCAG	3 cut(s)	51, 867, 878
BseNI	ACTGG	2 cut(s)	925, 946
BsePI	GCGCGC	1 cut(s)	394
BseRI	GAGGAG	4 cut(s)	130, 133, 671, 1322
BseXI	GCAGC	8 cut(s)	10, 16, 29, 32, 38, 326, 679, 867
BsgI	GTGCAG	1 cut(s)	732
Bsh1236I	CGCG	3 cut(s)	396, 793, 1338
Bsh1285I	CGRYCG	2 cut(s)	900, 1336
BshNI	GGYRCC	2 cut(s)	509, 527
BshTI	ACCGGT	1 cut(s)	1025
BshVI	ATCGAT	1 cut(s)	375
BsiEI	CGRYCG	2 cut(s)	900, 1336
BsiHKAI	GWGCWC	2 cut(s)	582, 663
BsiHKCI	CYCGRG	1 cut(s)	1142
BsiSI	CCGG	7 cut(s)	386, 474, 507, 1026, 1143, 1272, 1367
BslFI	GGGAC	2 cut(s)	70, 1159
BslI	CCNNNNNNNGG	2 cut(s)	325, 1143
BsmAI	GTCTC	4 cut(s)	358, 431, 547, 969
BsmBI	CGTCTC	2 cut(s)	358, 547
BsmFI	GGGAC	2 cut(s)	70, 1159
BsmI	GAATGC	1 cut(s)	1213
Bso31I	GGTCTC	1 cut(s)	431
BsoBI	CYCGRG	1 cut(s)	1142
Bsp1286I	GDGCHC	2 cut(s)	582, 663
Bsp143I	GATC	4 cut(s)	743, 897, 915, 1333
Bsp19I	CCATGG	2 cut(s)	324, 1062
Bsp68I	TCGCGA	1 cut(s)	793
BspCNI	CTCAG	3 cut(s)	50, 868, 877
BspDI	ATCGAT	1 cut(s)	375
BspFNI	CGCG	3 cut(s)	396, 793, 1338
BspLI	GGNNCC	3 cut(s)	511, 529, 550
BspMAI	CTGCAG	1 cut(s)	28
BspMI	ACCTGC	1 cut(s)	589
BspPI	GGATC	1 cut(s)	751
BspQI	GCTCTTC	1 cut(s)	384
BspT107I	GGYRCC	2 cut(s)	509, 527
BspTNI	GGTCTC	1 cut(s)	431
BsrFI	RCCGGY	2 cut(s)	506, 1025
BsrI	ACTGG	2 cut(s)	925, 946
BssAI	RCCGGY	2 cut(s)	506, 1025
BssECI	CCNNGG	9 cut(s)	292, 324, 330, 384, 400, 472, 925, 1062, 1142
BssHII	GCGCGC	1 cut(s)	394
BssMI	GATC	4 cut(s)	743, 897, 915, 1333
BssNI	GRCGYC	2 cut(s)	510, 528
BssT1I	CCWWGG	3 cut(s)	324, 330, 1062
Bst2UI	CCWGG	4 cut(s)	644, 717, 1009, 1329
Bst4CI	ACNGT	7 cut(s)	220, 427, 934, 982, 1021, 1221, 1420
Bst6I	CTCTTC	3 cut(s)	103, 384, 1194
BstACI	GRCGYC	2 cut(s)	510, 528
BstAPI	GCANNNNNTGC	3 cut(s)	23, 586, 755
BstC8I	GCNNGC	6 cut(s)	54, 396, 591, 756, 1375, 1379
BstDEI	CTNAG	4 cut(s)	37, 864, 876, 1091
BstDSI	CCRYGG	5 cut(s)	292, 324, 400, 925, 1062
BstF5I	GGATG	4 cut(s)	165, 1131, 1309, 1392
BstFNI	CGCG	3 cut(s)	396, 793, 1338
BstH2I	RGCGCY	2 cut(s)	513, 531
BstHHI	GCGC	5 cut(s)	249, 396, 398, 512, 530
BstKTI	GATC	4 cut(s)	746, 900, 918, 1336
BstMAI	GTCTC	4 cut(s)	358, 431, 547, 969
BstMBI	GATC	4 cut(s)	743, 897, 915, 1333
BstMCI	CGRYCG	2 cut(s)	900, 1336
BstNI	CCWGG	4 cut(s)	644, 717, 1009, 1329
BstSCI	CCNGG	9 cut(s)	384, 472, 642, 715, 1007, 1141, 1142, 1271, 1327
BstSFI	CTRYAG	1 cut(s)	24
BstUI	CGCG	3 cut(s)	396, 793, 1338
BstV1I	GCAGC	8 cut(s)	10, 16, 29, 32, 38, 326, 679, 867
BstV2I	GAAGAC	1 cut(s)	1421
BstX2I	RGATCY	1 cut(s)	743
BstXI	CCANNNNNNTGG	1 cut(s)	331
BstYI	RGATCY	1 cut(s)	743
Bsu15I	ATCGAT	1 cut(s)	375
BsuI	GTATCC	1 cut(s)	1109
BsuTUI	ATCGAT	1 cut(s)	375
BtgI	CCRYGG	5 cut(s)	292, 324, 400, 925, 1062
BtrI	CACGTC	2 cut(s)	286, 463
BtsCI	GGATG	4 cut(s)	165, 1131, 1309, 1392
BtsI	GCAGTG	1 cut(s)	749
BtsIMutI	CAGTG	4 cut(s)	749, 918, 930, 939
BtuMI	TCGCGA	1 cut(s)	793
BveI	ACCTGC	1 cut(s)	589
Cac8I	GCNNGC	6 cut(s)	54, 396, 591, 756, 1375, 1379
CaiI	CAGNNNCTG	1 cut(s)	23
CfoI	GCGC	5 cut(s)	249, 396, 398, 512, 530
Cfr10I	RCCGGY	2 cut(s)	506, 1025
Cfr13I	GGNCC	2 cut(s)	557, 640
Cfr9I	CCCGGG	1 cut(s)	1142
ClaI	ATCGAT	1 cut(s)	375
CseI	GACGC	1 cut(s)	340
CsiI	ACCWGGT	1 cut(s)	642
Csp6I	GTAC	1 cut(s)	433
CspAI	ACCGGT	1 cut(s)	1025
CviAII	CATG	6 cut(s)	260, 325, 499, 616, 1063, 1252
CviQI	GTAC	1 cut(s)	433
DdeI	CTNAG	4 cut(s)	37, 864, 876, 1091
DinI	GGCGCC	2 cut(s)	511, 529
DpnI	GATC	4 cut(s)	745, 899, 917, 1335
DpnII	GATC	4 cut(s)	743, 897, 915, 1333
EaeI	YGGCCR	6 cut(s)	280, 475, 922, 936, 943, 1134
Eam1104I	CTCTTC	3 cut(s)	103, 384, 1194
EarI	CTCTTC	3 cut(s)	103, 384, 1194
Ecl136II	GAGCTC	1 cut(s)	661
Eco130I	CCWWGG	3 cut(s)	324, 330, 1062
Eco24I	GRGCYC	1 cut(s)	663
Eco31I	GGTCTC	1 cut(s)	431
Eco32I	GATATC	1 cut(s)	1114
Eco47I	GGWCC	1 cut(s)	640
Eco53kI	GAGCTC	1 cut(s)	661
Eco88I	CYCGRG	1 cut(s)	1142
EcoICRI	GAGCTC	1 cut(s)	661
EcoRII	CCWGG	4 cut(s)	642, 715, 1007, 1327
EcoRV	GATATC	1 cut(s)	1114
EcoT14I	CCWWGG	3 cut(s)	324, 330, 1062
EcoT38I	GRGCYC	1 cut(s)	663
EgeI	GGCGCC	2 cut(s)	511, 529
EheI	GGCGCC	2 cut(s)	511, 529
ErhI	CCWWGG	3 cut(s)	324, 330, 1062
Esp3I	CGTCTC	2 cut(s)	358, 547
FaeI	CATG	6 cut(s)	263, 328, 502, 619, 1066, 1255
FaqI	GGGAC	2 cut(s)	70, 1159
FatI	CATG	6 cut(s)	259, 324, 498, 615, 1062, 1251
FauI	CCCGC	3 cut(s)	190, 203, 1164
FauNDI	CATATG	1 cut(s)	64
FblI	GTMKAC	3 cut(s)	288, 465, 984
FokI	GGATG	4 cut(s)	152, 1138, 1316, 1399
FriOI	GRGCYC	1 cut(s)	663
FspBI	CTAG	1 cut(s)	761
GlaI	GCGC	5 cut(s)	248, 395, 397, 511, 529
GsuI	CTGGAG	1 cut(s)	567
HaeII	RGCGCY	2 cut(s)	513, 531
HapII	CCGG	7 cut(s)	386, 474, 507, 1026, 1143, 1272, 1367
HgaI	GACGC	1 cut(s)	340
HhaI	GCGC	5 cut(s)	249, 396, 398, 512, 530
Hin1I	GRCGYC	2 cut(s)	510, 528
Hin1II	CATG	6 cut(s)	263, 328, 502, 619, 1066, 1255
Hin6I	GCGC	5 cut(s)	247, 394, 396, 510, 528
HinP1I	GCGC	5 cut(s)	247, 394, 396, 510, 528
HincII	GTYRAC	4 cut(s)	265, 289, 466, 985
HindII	GTYRAC	4 cut(s)	265, 289, 466, 985
HinfI	GANTC	5 cut(s)	405, 778, 836, 859, 1310
HpaII	CCGG	7 cut(s)	386, 474, 507, 1026, 1143, 1272, 1367
Hpy166II	GTNNAC	5 cut(s)	265, 289, 466, 649, 985
Hpy188I	TCNGA	5 cut(s)	858, 867, 974, 1044, 1403
Hpy188III	TCNNGA	9 cut(s)	493, 546, 628, 741, 792, 952, 1052, 1102, 1117
Hpy8I	GTNNAC	5 cut(s)	265, 289, 466, 649, 985
Hpy99I	CGWCG	9 cut(s)	290, 371, 431, 467, 542, 557, 656, 677, 986
HpyAV	CCTTC	8 cut(s)	126, 157, 955, 994, 1189, 1203, 1270, 1400
HpyCH4III	ACNGT	7 cut(s)	220, 427, 934, 982, 1021, 1221, 1420
HpyCH4IV	ACGT	3 cut(s)	285, 462, 489
HpyCH4V	TGCA	5 cut(s)	26, 32, 667, 749, 758
HpyF3I	CTNAG	4 cut(s)	37, 864, 876, 1091
HpySE526I	ACGT	3 cut(s)	285, 462, 489
Hsp92I	GRCGYC	2 cut(s)	510, 528
Hsp92II	CATG	6 cut(s)	263, 328, 502, 619, 1066, 1255
HspAI	GCGC	5 cut(s)	247, 394, 396, 510, 528
KasI	GGCGCC	2 cut(s)	509, 527
Kzo9I	GATC	4 cut(s)	743, 897, 915, 1333
LguI	GCTCTTC	1 cut(s)	384
LmnI	GCTCC	3 cut(s)	548, 658, 1424
Lsp1109I	GCAGC	8 cut(s)	10, 16, 29, 32, 38, 326, 679, 867
LweI	GCATC	3 cut(s)	367, 1048, 1314
MabI	ACCWGGT	1 cut(s)	642
MaeI	CTAG	1 cut(s)	761
MaeII	ACGT	3 cut(s)	285, 462, 489
MaeIII	GTNAC	5 cut(s)	1021, 1070, 1232, 1267, 1279
MalI	GATC	4 cut(s)	745, 899, 917, 1335
MboI	GATC	4 cut(s)	743, 897, 915, 1333
MflI	RGATCY	1 cut(s)	743
MhlI	GDGCHC	2 cut(s)	582, 663
MlsI	TGGCCA	2 cut(s)	938, 1136
MluCI	AATT	4 cut(s)	6, 710, 1047, 1166
MluNI	TGGCCA	2 cut(s)	938, 1136
Mly113I	GGCGCC	2 cut(s)	510, 528
MlyI	GAGTC	2 cut(s)	830, 1319
MmeI	TCCRAC	4 cut(s)	213, 660, 952, 1402
Mox20I	TGGCCA	2 cut(s)	938, 1136
MroXI	GAANNNNTTC	2 cut(s)	1038, 1213
MscI	TGGCCA	2 cut(s)	938, 1136
MseI	TTAA	2 cut(s)	966, 1341
Msp20I	TGGCCA	2 cut(s)	938, 1136
MspA1I	CMGCKG	2 cut(s)	23, 29
MspI	CCGG	7 cut(s)	386, 474, 507, 1026, 1143, 1272, 1367
MspR9I	CCNGG	9 cut(s)	386, 474, 644, 717, 1009, 1143, 1144, 1273, 1329
Mva1269I	GAATGC	1 cut(s)	1213
MvaI	CCWGG	4 cut(s)	644, 717, 1009, 1329
MvnI	CGCG	3 cut(s)	396, 793, 1338
NarI	GGCGCC	2 cut(s)	510, 528
NciI	CCSGG	5 cut(s)	386, 474, 1143, 1144, 1273
NcoI	CCATGG	2 cut(s)	324, 1062
NdeI	CATATG	1 cut(s)	64
NdeII	GATC	4 cut(s)	743, 897, 915, 1333
NlaIII	CATG	6 cut(s)	263, 328, 502, 619, 1066, 1255
NlaIV	GGNNCC	3 cut(s)	511, 529, 550
NmuCI	GTSAC	4 cut(s)	1021, 1070, 1267, 1279
NruI	TCGCGA	1 cut(s)	793
PaqCI	CACCTGC	1 cut(s)	589
PauI	GCGCGC	1 cut(s)	394
PciSI	GCTCTTC	1 cut(s)	384
PcsI	WCGNNNNNNNCGW	6 cut(s)	285, 348, 372, 462, 486, 978
PctI	GAATGC	1 cut(s)	1213
PdmI	GAANNNNTTC	2 cut(s)	1038, 1213
PfeI	GAWTC	3 cut(s)	405, 778, 859
PflFI	GACNNNGTC	1 cut(s)	980
PinAI	ACCGGT	1 cut(s)	1025
Ple19I	CGATCG	2 cut(s)	900, 1336
PleI	GAGTC	2 cut(s)	830, 1318
PluTI	GGCGCC	2 cut(s)	513, 531
PpsI	GAGTC	2 cut(s)	830, 1318
Psp124BI	GAGCTC	1 cut(s)	663
Psp6I	CCWGG	4 cut(s)	642, 715, 1007, 1327
PspGI	CCWGG	4 cut(s)	642, 715, 1007, 1327
PspN4I	GGNNCC	3 cut(s)	511, 529, 550
PspPI	GGNCC	2 cut(s)	557, 640
PsrI	GAACNNNNNNTAC	2 cut(s)	437, 469
PstI	CTGCAG	1 cut(s)	28
PstNI	CAGNNNCTG	1 cut(s)	23
PsuI	RGATCY	1 cut(s)	743
PsyI	GACNNNGTC	1 cut(s)	980
PteI	GCGCGC	1 cut(s)	394
PvuI	CGATCG	2 cut(s)	900, 1336
PvuII	CAGCTG	2 cut(s)	23, 29
RgaI	GCGATCGC	1 cut(s)	1336
RruI	TCGCGA	1 cut(s)	793
RsaI	GTAC	1 cut(s)	434
RsaNI	GTAC	1 cut(s)	433
SacI	GAGCTC	1 cut(s)	663
SalI	GTCGAC	3 cut(s)	287, 464, 983
SapI	GCTCTTC	1 cut(s)	384
SaqAI	TTAA	2 cut(s)	966, 1341
Sau3AI	GATC	4 cut(s)	743, 897, 915, 1333
Sau96I	GGNCC	2 cut(s)	557, 640
SchI	GAGTC	2 cut(s)	830, 1319
ScrFI	CCNGG	9 cut(s)	386, 474, 644, 717, 1009, 1143, 1144, 1273, 1329
SduI	GDGCHC	2 cut(s)	582, 663
SexAI	ACCWGGT	1 cut(s)	642
SfaAI	GCGATCGC	1 cut(s)	1336
SfaNI	GCATC	3 cut(s)	367, 1048, 1314
SfcI	CTRYAG	1 cut(s)	24
SfoI	GGCGCC	2 cut(s)	511, 529
SgfI	GCGATCGC	1 cut(s)	1336
SgrAI	CRCCGGYG	1 cut(s)	1025
SinI	GGWCC	1 cut(s)	640
SmaI	CCCGGG	1 cut(s)	1144
Sse9I	AATT	4 cut(s)	6, 710, 1047, 1166
SspDI	GGCGCC	2 cut(s)	509, 527
SspMI	CTAG	1 cut(s)	761
SstI	GAGCTC	1 cut(s)	663
StyD4I	CCNGG	9 cut(s)	384, 472, 642, 715, 1007, 1141, 1142, 1271, 1327
StyI	CCWWGG	3 cut(s)	324, 330, 1062
TaaI	ACNGT	7 cut(s)	220, 427, 934, 982, 1021, 1221, 1420
TaiI	ACGT	3 cut(s)	288, 465, 492
TaqII	GACCGA	1 cut(s)	455
TasI	AATT	4 cut(s)	6, 710, 1047, 1166
TauI	GCSGC	6 cut(s)	210, 230, 383, 515, 568, 1243
TfiI	GAWTC	3 cut(s)	405, 778, 859
Tru1I	TTAA	2 cut(s)	966, 1341
Tru9I	TTAA	2 cut(s)	966, 1341
TscAI	CASTG	4 cut(s)	756, 925, 937, 946
TseFI	GTSAC	4 cut(s)	1021, 1070, 1267, 1279
TseI	GCWGC	8 cut(s)	17, 20, 23, 26, 29, 314, 667, 880
Tsp45I	GTSAC	4 cut(s)	1021, 1070, 1267, 1279
TspDTI	ATGAA	3 cut(s)	723, 840, 1307
TspMI	CCCGGG	1 cut(s)	1142
TspRI	CASTG	4 cut(s)	756, 925, 937, 946
Tth111I	GACNNNGTC	1 cut(s)	980
VpaK11BI	GGWCC	1 cut(s)	640
XapI	RAATTY	3 cut(s)	710, 1047, 1166
XmaI	CCCGGG	1 cut(s)	1142
XmiI	GTMKAC	3 cut(s)	288, 465, 984
XmnI	GAANNNNTTC	2 cut(s)	1038, 1213
XspI	CTAG	1 cut(s)	761

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.