Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0004416

Physical Location & Seq

Genomic Coordinates Reverse (-)

2058737 .. 2060173

Gene Structure 1437 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00078950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGCTTCTGCTATCGGAGCCCCACCCTCGTCAAAACTCCTCTTCACTCCCTCACCCACCACAACCTCACTTTCTCATCCCACCCACCTTTCTCTCTCTACACCCAAAGCCCTAGCATCAAAGCCCCTCTTCTCCTCCTCCTCCGCCGCCTTCGATGTCGTCGTCGTCTCCCCCTCTCTCCGGAGGCGCCGATCCTTCACCGTCCGGATGGCTCGTGGCAAGTTCGAGCGGAAGAAGCCCCACGTCAACATCGGCACCATCGGCCACGTCGACCACGGCAAGACCACCCTCACCGCCGCCCTCACCATGGCCCTGGCCTCCATGGGGAACAGCGCCCCCAAGAAGTACGACGAGATCGACGCCGCGCCGGAGGAGCGCGCGCGCGGCATCACCATCAACACCGCCACCGTCGAGTACGAGACCGCCACGCGCCACTACGCGCACGTCGACTGCCCCGGCCACGCCGACTACGTCAAGAACATGATCACCGGGGCCGCGCAGATGGACGGCGCCATCCTCGTCGTCTCCGGCGCCGACGGCCCGATGCCGCAGACGAAGGAGCACGTCCTGCTGGCCAAGCAGGTCGGCGTGCCCAATGTGGTGGTGTTCTTGAACAAACAGGACCAGGTGGACGACGCCGAGCTGCTTGAGCTGGTGGAGTTGGAGGTCAGGGACTTGTTATCGTCCTATGAATTCCCCGGCGACGATGTTCCGGTGGTTTCGGGTTCGGCATTGCTGGCATTGGAGGCGTTAATGGCGAACCCGAAAATAAAGAGAGGGGAGAATGAATGGGTGGATAAAATTTATGACTTGATGGATTGTGTGGATGACTACATTCCAATTCCGCAGAGGCAGACTGATCTGCCGTTTCTGTTGGCCGTTGAGGATGTGTTTTCGATCACAGGACGTGGCACGGTGGCCACGGGGCGGGTGGAGAGAGGTAAAGTGAAGGTTGGGGACCATGTGGATGTAGTGGGATTGAGAGAGACTAGGAATACAACTGTCACCGGAGTTGAAATGTTTCAGAAAACACTTGATGAGGCACTTGCTGGTGACAATGTAGGGCTTTTGCTGAGGGGTATTCAGAAGATGGATATTCAGAGAGGGATGGTGGTTTCTAAGCCGGGTAGCATCACACCTCACACTAAGTTTGAGGCGATTGTGTATGTGTTGAAGAAGGAAGAGGGTGGGAGGCATTCACCGTTCTTTGCAGGGTATAGGCCTCAGTTTTACATGAGGACTACTGATGTGACCGGGAAGGTGACGCAGATTATGAATGACAGTGATGAGGTGTCGAAGATGGTTATGCCGGGAGACAGAGTGAAGATGGTGGTCGAGCTTATAATGCCGGTGGCTTGTGAGCAAGGGATGAGATTTGCCATTAGGGAAGGTGGCAAGACTGTTGGGGCTGGTGTCATTCAGTCTATTATTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

478

Amino Acids

51.91

Weight (kDa)

6.48

Isoelectric Point (pI)

34.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	79 - 280	1.1e-61	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	304 - 372	8.6e-19	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	377 - 476	4.8e-40	Elongation factor Tu C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20360 AT4G20360
fragaria_vesca	FvH4_5g06350 FvH4_6g51880
malus_domestica	MD06G1165400.v1.1 MD09G1022300.v1.1 MD14G1170900.v1.1
prunus_persica	Prupe.3G294300_v2.0.a1 Prupe.5G165000_v2.0.a1 Prupe.5G165000_v2.0.a1
pyrus_communis	pycom06g14780 pycom111g01870 pycom14g14280
rosa_chinensis	RchiOBHm_Chr2g0173371 RchiOBHm_Chr7g0192491
rosa_laevigata	RLG00000004361 RLG00000022183
rosa_multiflora	Rmu_sc0003108.1_g000029 Rmu_sc0014554.1_g000001
rosa_roxburghii	Rroxscaffold_2G00078950 Rroxscaffold_3G00262980
rosa_rugosa	Rorug02G0567600 Rorug07G0001900
rosa_samantha	Rh2AG648100 Rh2BG658700 Rh2DG674100 Rh7AG125900 Rh7BG128200 Rh7CG129800 Rh7DG129700
rosa_wichuraiana	Rw2G053200 Rw7G010840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1343
Acc36I	ACCTGC	1 cut(s)	571
AccB1I	GGYRCC	4 cut(s)	186, 254, 509, 530
AccBSI	CCGCTC	1 cut(s)	229
AccI	GTMKAC	2 cut(s)	270, 447
AccII	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
AccIII	TCCGGA	2 cut(s)	180, 204
AclWI	GGATC	1 cut(s)	186
AcoI	YGGCCR	5 cut(s)	262, 457, 573, 876, 918
AcsI	RAATTY	2 cut(s)	692, 801
AcyI	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
AfaI	GTAC	2 cut(s)	347, 416
AfiI	CCNNNNNNNGG	4 cut(s)	180, 307, 324, 927
AgsI	TTSAA	3 cut(s)	613, 1016, 1174
AjiI	CACGTC	5 cut(s)	244, 268, 445, 565, 908
AjnI	CCWGG	2 cut(s)	312, 624
AluBI	AGCT	3 cut(s)	643, 652, 1339
AluI	AGCT	3 cut(s)	643, 652, 1339
Alw21I	GWGCWC	1 cut(s)	564
Alw26I	GTCTC	5 cut(s)	172, 413, 529, 980, 1308
AlwI	GGATC	1 cut(s)	186
Aor13HI	TCCGGA	2 cut(s)	180, 204
ApeKI	GCWGC	1 cut(s)	643
ApoI	RAATTY	2 cut(s)	692, 801
Asp700I	GAANNNNTTC	1 cut(s)	1020
AspS9I	GGNCC	5 cut(s)	310, 492, 539, 622, 958
AsuC2I	CCSGG	6 cut(s)	456, 490, 699, 1125, 1255, 1311
AvaII	GGWCC	2 cut(s)	622, 958
BaeGI	GKGCMC	1 cut(s)	594
BalI	TGGCCA	2 cut(s)	575, 920
BanI	GGYRCC	4 cut(s)	186, 254, 509, 530
BanII	GRGCYC	1 cut(s)	22
BauI	CACGAG	1 cut(s)	213
Bbv12I	GWGCWC	1 cut(s)	564
BbvCI	CCTCAGC	1 cut(s)	1073
BbvI	GCAGC	1 cut(s)	630
BceAI	ACGGC	5 cut(s)	292, 523, 553, 850, 863
BciT130I	CCWGG	2 cut(s)	314, 626
BclI	TGATCA	1 cut(s)	483
BcnI	CCSGG	6 cut(s)	456, 490, 699, 1125, 1255, 1311
BcoDI	GTCTC	5 cut(s)	172, 413, 529, 980, 1308
BfaI	CTAG	2 cut(s)	113, 990
BfoI	RGCGCY	4 cut(s)	190, 336, 513, 534
BfuAI	ACCTGC	1 cut(s)	571
BisI	GCNGC	7 cut(s)	147, 297, 363, 385, 495, 548, 644
BlsI	GCNGC	7 cut(s)	148, 298, 364, 386, 496, 549, 645
Bme1390I	CCNGG	8 cut(s)	314, 456, 490, 626, 699, 1125, 1255, 1311
Bme18I	GGWCC	2 cut(s)	622, 958
BmgBI	CACGTC	5 cut(s)	244, 268, 445, 565, 908
BmgT120I	GGNCC	5 cut(s)	310, 492, 539, 622, 958
BmiI	GGNNCC	7 cut(s)	19, 188, 256, 493, 511, 532, 959
BmrFI	CCNGG	8 cut(s)	314, 456, 490, 626, 699, 1125, 1255, 1311
BmsI	GCATC	4 cut(s)	125, 396, 534, 1140
Bpu10I	CCTNAGC	1 cut(s)	1073
BpuEI	CTTGAG	1 cut(s)	668
BpuMI	CCSGG	6 cut(s)	456, 490, 699, 1125, 1255, 1311
BsaHI	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
BsaI	GGTCTC	1 cut(s)	413
BsaJI	CCNNGG	8 cut(s)	274, 306, 312, 321, 454, 489, 697, 921
BsaWI	WCCGGW	4 cut(s)	180, 204, 712, 1007
BsaXI	ACNNNNNCTCC	2 cut(s)	1305, 1335
Bsc4I	CCNNNNNNNGG	4 cut(s)	180, 307, 324, 927
Bse118I	RCCGGY	1 cut(s)	1348
Bse3DI	GCAATG	1 cut(s)	731
BseAI	TCCGGA	2 cut(s)	180, 204
BseBI	CCWGG	2 cut(s)	314, 626
BseDI	CCNNGG	8 cut(s)	274, 306, 312, 321, 454, 489, 697, 921
BseGI	GGATG	8 cut(s)	76, 213, 513, 832, 892, 973, 1113, 1374
BseLI	CCNNNNNNNGG	4 cut(s)	180, 307, 324, 927
BseMI	GCAATG	1 cut(s)	731
BseMII	CTCAG	2 cut(s)	1064, 1238
BsePI	GCGCGC	3 cut(s)	376, 378, 380
BseRI	GAGGAG	5 cut(s)	29, 124, 127, 130, 386
BseSI	GKGCMC	1 cut(s)	594
BseXI	GCAGC	1 cut(s)	630
Bsh1236I	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
BshNI	GGYRCC	4 cut(s)	186, 254, 509, 530
BsiHKAI	GWGCWC	1 cut(s)	564
BslFI	GGGAC	2 cut(s)	686, 971
BslI	CCNNNNNNNGG	4 cut(s)	180, 307, 324, 927
BsmAI	GTCTC	5 cut(s)	172, 413, 529, 980, 1308
BsmBI	CGTCTC	2 cut(s)	172, 529
BsmFI	GGGAC	2 cut(s)	686, 971
BsmI	GAATGC	1 cut(s)	1195
Bso31I	GGTCTC	1 cut(s)	413
Bsp1286I	GDGCHC	3 cut(s)	22, 564, 594
Bsp13I	TCCGGA	2 cut(s)	180, 204
Bsp143I	GATC	5 cut(s)	191, 354, 483, 859, 897
Bsp19I	CCATGG	2 cut(s)	306, 321
BspCNI	CTCAG	2 cut(s)	1065, 1237
BspEI	TCCGGA	2 cut(s)	180, 204
BspFNI	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
BspLI	GGNNCC	7 cut(s)	19, 188, 256, 493, 511, 532, 959
BspMI	ACCTGC	1 cut(s)	571
BspPI	GGATC	1 cut(s)	186
BspT107I	GGYRCC	4 cut(s)	186, 254, 509, 530
BspTNI	GGTCTC	1 cut(s)	413
BsrBI	CCGCTC	1 cut(s)	229
BsrDI	GCAATG	1 cut(s)	731
BsrFI	RCCGGY	1 cut(s)	1348
BssAI	RCCGGY	1 cut(s)	1348
BssECI	CCNNGG	8 cut(s)	274, 306, 312, 321, 454, 489, 697, 921
BssHII	GCGCGC	3 cut(s)	376, 378, 380
BssMI	GATC	5 cut(s)	191, 354, 483, 859, 897
BssNI	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
BssSI	CACGAG	1 cut(s)	213
BssT1I	CCWWGG	2 cut(s)	306, 321
Bst2BI	CACGAG	1 cut(s)	213
Bst2UI	CCWGG	2 cut(s)	314, 626
Bst4CI	ACNGT	7 cut(s)	202, 409, 916, 1003, 1203, 1283, 1402
Bst6I	CTCTTC	3 cut(s)	47, 134, 1176
BstACI	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
BstAPI	GCANNNNNTGC	1 cut(s)	568
BstC8I	GCNNGC	6 cut(s)	378, 380, 382, 573, 590, 738
BstDEI	CTNAG	4 cut(s)	1073, 1119, 1146, 1224
BstDSI	CCRYGG	4 cut(s)	274, 306, 321, 921
BstF5I	GGATG	8 cut(s)	76, 213, 513, 832, 892, 973, 1113, 1374
BstFNI	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
BstH2I	RGCGCY	4 cut(s)	190, 336, 513, 534
BstKTI	GATC	5 cut(s)	194, 357, 486, 862, 900
BstMAI	GTCTC	5 cut(s)	172, 413, 529, 980, 1308
BstMBI	GATC	5 cut(s)	191, 354, 483, 859, 897
BstNI	CCWGG	2 cut(s)	314, 626
BstSCI	CCNGG	8 cut(s)	312, 454, 488, 624, 697, 1123, 1253, 1309
BstSLI	GKGCMC	1 cut(s)	594
BstUI	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
BstV1I	GCAGC	1 cut(s)	630
BstXI	CCANNNNNNTGG	2 cut(s)	313, 601
BtgI	CCRYGG	4 cut(s)	274, 306, 321, 921
BtrI	CACGTC	5 cut(s)	244, 268, 445, 565, 908
BtsCI	GGATG	8 cut(s)	76, 213, 513, 832, 892, 973, 1113, 1374
BtsIMutI	CAGTG	1 cut(s)	1288
BveI	ACCTGC	1 cut(s)	571
Cac8I	GCNNGC	6 cut(s)	378, 380, 382, 573, 590, 738
Cfr10I	RCCGGY	1 cut(s)	1348
Cfr13I	GGNCC	5 cut(s)	310, 492, 539, 622, 958
CseI	GACGC	3 cut(s)	368, 644, 1273
CsiI	ACCWGGT	1 cut(s)	624
Csp6I	GTAC	2 cut(s)	346, 415
CviAII	CATG	5 cut(s)	307, 322, 481, 962, 1234
CviQI	GTAC	2 cut(s)	346, 415
DdeI	CTNAG	4 cut(s)	1073, 1119, 1146, 1224
DinI	GGCGCC	3 cut(s)	188, 511, 532
DpnI	GATC	5 cut(s)	193, 356, 485, 861, 899
DpnII	GATC	5 cut(s)	191, 354, 483, 859, 897
EaeI	YGGCCR	5 cut(s)	262, 457, 573, 876, 918
Eam1104I	CTCTTC	3 cut(s)	47, 134, 1176
EarI	CTCTTC	3 cut(s)	47, 134, 1176
EciI	GGCGGA	1 cut(s)	133
Eco130I	CCWWGG	2 cut(s)	306, 321
Eco147I	AGGCCT	1 cut(s)	1222
Eco24I	GRGCYC	1 cut(s)	22
Eco31I	GGTCTC	1 cut(s)	413
Eco47I	GGWCC	2 cut(s)	622, 958
EcoRI	GAATTC	1 cut(s)	692
EcoRII	CCWGG	2 cut(s)	312, 624
EcoT14I	CCWWGG	2 cut(s)	306, 321
EcoT38I	GRGCYC	1 cut(s)	22
EgeI	GGCGCC	3 cut(s)	188, 511, 532
EheI	GGCGCC	3 cut(s)	188, 511, 532
ErhI	CCWWGG	2 cut(s)	306, 321
Esp3I	CGTCTC	2 cut(s)	172, 529
FaeI	CATG	5 cut(s)	310, 325, 484, 965, 1237
FalI	AAGNNNNNCTT	2 cut(s)	113, 145
FaqI	GGGAC	2 cut(s)	686, 971
FatI	CATG	5 cut(s)	306, 321, 480, 961, 1233
FauI	CCCGC	1 cut(s)	921
FbaI	TGATCA	1 cut(s)	483
FblI	GTMKAC	2 cut(s)	270, 447
Fnu4HI	GCNGC	7 cut(s)	147, 297, 363, 385, 495, 548, 644
FokI	GGATG	8 cut(s)	63, 220, 500, 839, 899, 980, 1120, 1381
FriOI	GRGCYC	1 cut(s)	22
Fsp4HI	GCNGC	7 cut(s)	147, 297, 363, 385, 495, 548, 644
FspBI	CTAG	2 cut(s)	113, 990
GluI	GCNGC	7 cut(s)	147, 297, 363, 385, 495, 548, 644
HaeII	RGCGCY	4 cut(s)	190, 336, 513, 534
HgaI	GACGC	3 cut(s)	368, 644, 1273
Hin1I	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
Hin1II	CATG	5 cut(s)	310, 325, 484, 965, 1237
HincII	GTYRAC	3 cut(s)	247, 271, 448
HindII	GTYRAC	3 cut(s)	247, 271, 448
Hpy166II	GTNNAC	4 cut(s)	247, 271, 448, 631
Hpy188I	TCNGA	4 cut(s)	17, 1026, 1086, 1101
Hpy188III	TCNNGA	4 cut(s)	181, 205, 475, 610
Hpy8I	GTNNAC	4 cut(s)	247, 271, 448, 631
HpyAV	CCTTC	7 cut(s)	160, 205, 550, 943, 1171, 1252, 1382
HpyCH4III	ACNGT	7 cut(s)	202, 409, 916, 1003, 1203, 1283, 1402
HpyCH4IV	ACGT	6 cut(s)	243, 267, 444, 471, 564, 907
HpyCH4V	TGCA	1 cut(s)	1211
HpyF3I	CTNAG	4 cut(s)	1073, 1119, 1146, 1224
HpySE526I	ACGT	6 cut(s)	243, 267, 444, 471, 564, 907
Hsp92I	GRCGYC	5 cut(s)	187, 360, 510, 531, 636
Hsp92II	CATG	5 cut(s)	310, 325, 484, 965, 1237
KasI	GGCGCC	3 cut(s)	186, 509, 530
Kpn2I	TCCGGA	2 cut(s)	180, 204
Ksp22I	TGATCA	1 cut(s)	483
Kzo9I	GATC	5 cut(s)	191, 354, 483, 859, 897
LmnI	GCTCC	3 cut(s)	17, 373, 559
Lsp1109I	GCAGC	1 cut(s)	630
LweI	GCATC	4 cut(s)	125, 396, 534, 1140
MabI	ACCWGGT	1 cut(s)	624
MaeI	CTAG	2 cut(s)	113, 990
MaeII	ACGT	6 cut(s)	243, 267, 444, 471, 564, 907
MaeIII	GTNAC	4 cut(s)	1003, 1052, 1249, 1261
MalI	GATC	5 cut(s)	193, 356, 485, 861, 899
MauBI	CGCGCGCG	2 cut(s)	378, 380
MbiI	CCGCTC	1 cut(s)	229
MboI	GATC	5 cut(s)	191, 354, 483, 859, 897
MboII	GAAGA	8 cut(s)	34, 121, 244, 1099, 1186, 1193, 1309, 1336
MhlI	GDGCHC	3 cut(s)	22, 564, 594
MlsI	TGGCCA	2 cut(s)	575, 920
MluCI	AATT	3 cut(s)	692, 801, 840
MluNI	TGGCCA	2 cut(s)	575, 920
Mly113I	GGCGCC	3 cut(s)	187, 510, 531
MmeI	TCCRAC	1 cut(s)	642
Mox20I	TGGCCA	2 cut(s)	575, 920
MroI	TCCGGA	2 cut(s)	180, 204
MroXI	GAANNNNTTC	1 cut(s)	1020
MscI	TGGCCA	2 cut(s)	575, 920
MseI	TTAA	1 cut(s)	752
MslI	CAYNNNNRTG	1 cut(s)	966
Msp20I	TGGCCA	2 cut(s)	575, 920
MspR9I	CCNGG	8 cut(s)	314, 456, 490, 626, 699, 1125, 1255, 1311
Mva1269I	GAATGC	1 cut(s)	1195
MvaI	CCWGG	2 cut(s)	314, 626
MvnI	CGCG	8 cut(s)	365, 378, 380, 382, 384, 430, 440, 497
NarI	GGCGCC	3 cut(s)	187, 510, 531
NciI	CCSGG	6 cut(s)	456, 490, 699, 1125, 1255, 1311
NcoI	CCATGG	2 cut(s)	306, 321
NdeII	GATC	5 cut(s)	191, 354, 483, 859, 897
NlaIII	CATG	5 cut(s)	310, 325, 484, 965, 1237
NlaIV	GGNNCC	7 cut(s)	19, 188, 256, 493, 511, 532, 959
NmeAIII	GCCGAG	1 cut(s)	664
NmuCI	GTSAC	4 cut(s)	1003, 1052, 1249, 1261
PauI	GCGCGC	3 cut(s)	376, 378, 380
PceI	AGGCCT	1 cut(s)	1222
PcsI	WCGNNNNNNNCGW	5 cut(s)	159, 267, 354, 444, 468
PctI	GAATGC	1 cut(s)	1195
PdmI	GAANNNNTTC	1 cut(s)	1020
PflFI	GACNNNGTC	1 cut(s)	470
PkrI	GCNGC	7 cut(s)	148, 298, 364, 386, 496, 549, 645
PluTI	GGCGCC	3 cut(s)	190, 513, 534
PsiI	TTATAA	1 cut(s)	1343
Psp6I	CCWGG	2 cut(s)	312, 624
PspGI	CCWGG	2 cut(s)	312, 624
PspN4I	GGNNCC	7 cut(s)	19, 188, 256, 493, 511, 532, 959
PspPI	GGNCC	5 cut(s)	310, 492, 539, 622, 958
PsyI	GACNNNGTC	1 cut(s)	470
PteI	GCGCGC	3 cut(s)	376, 378, 380
RsaI	GTAC	2 cut(s)	347, 416
RsaNI	GTAC	2 cut(s)	346, 415
RseI	CAYNNNNRTG	1 cut(s)	966
SalI	GTCGAC	2 cut(s)	269, 446
SaqAI	TTAA	1 cut(s)	752
SatI	GCNGC	7 cut(s)	147, 297, 363, 385, 495, 548, 644
Sau3AI	GATC	5 cut(s)	191, 354, 483, 859, 897
Sau96I	GGNCC	5 cut(s)	310, 492, 539, 622, 958
ScrFI	CCNGG	8 cut(s)	314, 456, 490, 626, 699, 1125, 1255, 1311
SduI	GDGCHC	3 cut(s)	22, 564, 594
SexAI	ACCWGGT	1 cut(s)	624
SfaNI	GCATC	4 cut(s)	125, 396, 534, 1140
SfoI	GGCGCC	3 cut(s)	188, 511, 532
SinI	GGWCC	2 cut(s)	622, 958
SmiMI	CAYNNNNRTG	1 cut(s)	966
SmlI	CTYRAG	1 cut(s)	647
SmoI	CTYRAG	1 cut(s)	647
Sse9I	AATT	3 cut(s)	692, 801, 840
SseBI	AGGCCT	1 cut(s)	1222
SspDI	GGCGCC	3 cut(s)	186, 509, 530
SspMI	CTAG	2 cut(s)	113, 990
StuI	AGGCCT	1 cut(s)	1222
StyD4I	CCNGG	8 cut(s)	312, 454, 488, 624, 697, 1123, 1253, 1309
StyI	CCWWGG	2 cut(s)	306, 321
TaaI	ACNGT	7 cut(s)	202, 409, 916, 1003, 1203, 1283, 1402
TaiI	ACGT	6 cut(s)	246, 270, 447, 474, 567, 910
TaqI	TCGA	9 cut(s)	153, 225, 270, 357, 411, 447, 896, 1295, 1335
TasI	AATT	3 cut(s)	692, 801, 840
TauI	GCSGC	6 cut(s)	149, 299, 365, 387, 497, 550
Tru1I	TTAA	1 cut(s)	752
Tru9I	TTAA	1 cut(s)	752
TscAI	CASTG	1 cut(s)	1288
TseFI	GTSAC	4 cut(s)	1003, 1052, 1249, 1261
TseI	GCWGC	1 cut(s)	643
Tsp45I	GTSAC	4 cut(s)	1003, 1052, 1249, 1261
TspDTI	ATGAA	3 cut(s)	705, 801, 1289
TspRI	CASTG	1 cut(s)	1288
Tth111I	GACNNNGTC	1 cut(s)	470
VpaK11BI	GGWCC	2 cut(s)	622, 958
XapI	RAATTY	2 cut(s)	692, 801
XcmI	CCANNNNNNNNNTGG	1 cut(s)	928
XmiI	GTMKAC	2 cut(s)	270, 447
XmnI	GAANNNNTTC	1 cut(s)	1020
XspI	CTAG	2 cut(s)	113, 990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.