MD06G1191700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009028
Physical Location & Seq
Reverse (-)
32743885 .. 32747026
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1191700.v1.1.491

Sequence Viewer

Length: 930 bp
ATGCATCGCTCCAAGACCGTAGTTTTCATCTGGTTCCTCTACGCCTTGCTCTTCTACTCTCTCTTCCAAATGGCTCTCCGGAACTCCGACTCCTCACGCAATTCCACCTCTGCCTCTGATTCTGTAATTTCGAACAAGGAGCGGAGGTCCGCACTGTACGATCAGATGGGTCGGGATTTGGCTGAGCACGGACCGGTTTTTCTCAAAAACGGCGGCCAGACCTCCCAGTCTCTGTCACTTTCTGATATTTTTACTATCAGGGATGGATCTGTAGCACCTGTGCTTAAGGCAGCAAATCCTCCTGTTCGTGCTATTGTGTTGTATCTGAGCACACAGTACTCGATTCCGATTTTGAATGCGCTCAAACCTATACTCGATCCTTACTTTGATGAAGCGATCTGGTTTCAGAACTCGAGCTTATATCATTCTAGTATGTTTCATGCCTCGCATCATATTTCACCTGTTCCTGCTACTCCAGATGAGATTGAAGCTGAAGCAGCTGCCGTTAGGAATATTGCAGAGGGTCTCTGCCCTTTGAAAATTGTCTTGGACAGGGTGGTCTTAACTTCAACGGGTGTGCTTCTTGGGTGCTGGCAGGTAACCTCTGGATCAGATCCTATAACCATTCGAGAAAAATTGAGGACCGCGCTTCCACGTGCACCAGAAAAGCAACTTTATGATGTTGCTATTCTTCATACTTCATTTGCAAGGCTTCTTGGTCACCCCAAAGCATCATCCACGGTGCTATCTAATACTTCAGAACAAGTCCACTTCTTCCAAGAGCTTGTTAATCGTCTAAACAAAGAACTCCAAGGATTTGAGGCTGTGGTATCTGAGCTCTGGTATGTCGAAGAATTTGACGTTTTGGCTCTAGCATTGAACGGAAGAATGAAGTTTCGCAAGTTTCAACTTGGCTGCTCGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.5

Weight (kDa)

7.78

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 226, 557
Acc36I ACCTGC 1 cut(s) 586
AccBSI CCGCTC 1 cut(s) 142
AccII CGCG 1 cut(s) 647
AccIII TCCGGA 1 cut(s) 78
AciI CCGC 4 cut(s) 142, 150, 213, 645
AclWI GGATC 4 cut(s) 274, 371, 608, 616
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 854
AcuI CTGAAG 2 cut(s) 513, 741
AcvI CACGTG 1 cut(s) 656
AfaI GTAC 2 cut(s) 158, 338
AflII CTTAAG 1 cut(s) 284
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 6 cut(s) 355, 488, 538, 570, 880, 908
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 5 cut(s) 417, 491, 500, 784, 838
AluI AGCT 5 cut(s) 417, 491, 500, 784, 838
Alw21I GWGCWC 4 cut(s) 189, 332, 661, 840
Alw26I GTCTC 2 cut(s) 234, 530
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 4 cut(s) 274, 371, 608, 616
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 2 cut(s) 412, 919
Aor13HI TCCGGA 1 cut(s) 78
AoxI GGCC 1 cut(s) 214
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 4 cut(s) 290, 497, 500, 915
ApoI RAATTY 1 cut(s) 854
AsiGI ACCGGT 1 cut(s) 193
AspLEI GCGC 2 cut(s) 361, 649
AspS9I GGNCC 3 cut(s) 147, 191, 642
AsuHPI GGTGA 2 cut(s) 450, 713
AsuII TTCGAA 1 cut(s) 131
AvaI CYCGRG 2 cut(s) 412, 919
AvaII GGWCC 3 cut(s) 147, 191, 642
BaeGI GKGCMC 1 cut(s) 661
BanII GRGCYC 1 cut(s) 840
BbrPI CACGTG 1 cut(s) 656
Bbv12I GWGCWC 4 cut(s) 189, 332, 661, 840
BbvI GCAGC 4 cut(s) 302, 487, 509, 902
BccI CCATC 2 cut(s) 160, 257
BceAI ACGGC 2 cut(s) 226, 488
BcoDI GTCTC 2 cut(s) 234, 530
BfaI CTAG 2 cut(s) 429, 872
BfmI CTRYAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 284
BfuAI ACCTGC 1 cut(s) 586
BisI GCNGC 5 cut(s) 214, 291, 498, 501, 916
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 5 cut(s) 215, 292, 499, 502, 917
BmcAI AGTACT 1 cut(s) 338
Bme18I GGWCC 3 cut(s) 147, 191, 642
BmeT110I CYCGRG 2 cut(s) 412, 919
BmgT120I GGNCC 3 cut(s) 147, 191, 642
BmiI GGNNCC 1 cut(s) 35
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 3 cut(s) 13, 457, 740
BmuI ACTGGG 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 738, 811
BsaWI WCCGGW 2 cut(s) 78, 193
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 131, 161
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 226
BseAI TCCGGA 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 738, 811
BseGI GGATG 2 cut(s) 268, 734
BseMII CTCAG 3 cut(s) 174, 317, 825
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 4 cut(s) 302, 487, 509, 902
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 216
BshTI ACCGGT 1 cut(s) 193
BsiHKAI GWGCWC 4 cut(s) 189, 332, 661, 840
BsiHKCI CYCGRG 2 cut(s) 412, 919
BsiSI CCGG 2 cut(s) 79, 194
BsmAI GTCTC 2 cut(s) 234, 530
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 216
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 412, 919
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 4 cut(s) 189, 332, 661, 840
Bsp13I TCCGGA 1 cut(s) 78
Bsp143I GATC 6 cut(s) 160, 266, 376, 396, 608, 613
Bsp1720I GCTNAGC 1 cut(s) 183
BspACI CCGC 4 cut(s) 142, 150, 213, 645
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 3 cut(s) 175, 318, 826
BspEI TCCGGA 1 cut(s) 78
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 1 cut(s) 35
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 4 cut(s) 274, 371, 608, 616
BspQI GCTCTTC 1 cut(s) 56
BspT104I TTCGAA 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 530
BsrBI CCGCTC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 738, 811
BssMI GATC 6 cut(s) 160, 266, 376, 396, 608, 613
BssT1I CCWWGG 1 cut(s) 811
Bst4CI ACNGT 4 cut(s) 19, 156, 336, 742
Bst6I CTCTTC 2 cut(s) 56, 68
BstAFI CTTAAG 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 3 cut(s) 183, 326, 834
BstDSI CCRYGG 1 cut(s) 738
BstEII GGTNACC 2 cut(s) 598, 719
BstF5I GGATG 2 cut(s) 268, 734
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 2 cut(s) 361, 649
BstKTI GATC 6 cut(s) 163, 269, 379, 399, 611, 616
BstMAI GTCTC 2 cut(s) 234, 530
BstMBI GATC 6 cut(s) 160, 266, 376, 396, 608, 613
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstPI GGTNACC 2 cut(s) 598, 719
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 4 cut(s) 302, 487, 509, 902
BstX2I RGATCY 2 cut(s) 266, 613
BstYI RGATCY 2 cut(s) 266, 613
BsuRI GGCC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 738
BtsCI GGATG 2 cut(s) 268, 734
BtsIMutI CAGTG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 1 cut(s) 593
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 2 cut(s) 361, 649
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 147, 191, 642
CpoI CGGWCCG 1 cut(s) 191
Csp6I GTAC 2 cut(s) 157, 337
CspAI ACCGGT 1 cut(s) 193
CspI CGGWCCG 1 cut(s) 191
CviAII CATG 1 cut(s) 440
CviQI GTAC 2 cut(s) 157, 337
DdeI CTNAG 3 cut(s) 183, 326, 834
DpnI GATC 6 cut(s) 162, 268, 378, 398, 610, 615
DpnII GATC 6 cut(s) 160, 266, 376, 396, 608, 613
DrdI GACNNNNNNGTC 2 cut(s) 226, 557
DseDI GACNNNNNNGTC 2 cut(s) 226, 557
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 56, 68
EarI CTCTTC 2 cut(s) 56, 68
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 3 cut(s) 147, 191, 642
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 513, 741
Eco72I CACGTG 1 cut(s) 656
Eco88I CYCGRG 2 cut(s) 412, 919
Eco91I GGTNACC 2 cut(s) 598, 719
EcoICRI GAGCTC 1 cut(s) 838
EcoO65I GGTNACC 2 cut(s) 598, 719
EcoT14I CCWWGG 1 cut(s) 811
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 1 cut(s) 443
FaiI YATR 9 cut(s) 371, 421, 434, 441, 453, 620, 678, 696, 846
FatI CATG 1 cut(s) 439
Fnu4HI GCNGC 5 cut(s) 214, 291, 498, 501, 916
FokI GGATG 2 cut(s) 275, 721
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 5 cut(s) 214, 291, 498, 501, 916
FspBI CTAG 2 cut(s) 429, 872
GlaI GCGC 2 cut(s) 360, 648
GluI GCNGC 5 cut(s) 214, 291, 498, 501, 916
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 1 cut(s) 216
HapII CCGG 2 cut(s) 79, 194
HhaI GCGC 2 cut(s) 361, 649
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 2 cut(s) 359, 647
HinP1I GCGC 2 cut(s) 359, 647
HinfI GANTC 3 cut(s) 89, 119, 343
HpaII CCGG 2 cut(s) 79, 194
HphI GGTGA 2 cut(s) 450, 713
Hpy166II GTNNAC 2 cut(s) 659, 769
Hpy188III TCNNGA 6 cut(s) 79, 173, 476, 606, 629, 921
Hpy8I GTNNAC 2 cut(s) 659, 769
HpyCH4III ACNGT 4 cut(s) 19, 156, 336, 742
HpyCH4IV ACGT 2 cut(s) 655, 861
HpyCH4V TGCA 4 cut(s) 4, 518, 659, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 3 cut(s) 183, 326, 834
HpySE526I ACGT 2 cut(s) 655, 861
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 2 cut(s) 359, 647
Kpn2I TCCGGA 1 cut(s) 78
Kzo9I GATC 6 cut(s) 160, 266, 376, 396, 608, 613
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 14, 139
Lsp1109I GCAGC 4 cut(s) 302, 487, 509, 902
LweI GCATC 3 cut(s) 13, 457, 740
MaeI CTAG 2 cut(s) 429, 872
MaeII ACGT 2 cut(s) 655, 861
MaeIII GTNAC 3 cut(s) 234, 598, 719
MalI GATC 6 cut(s) 162, 268, 378, 398, 610, 615
MbiI CCGCTC 1 cut(s) 142
MboI GATC 6 cut(s) 160, 266, 376, 396, 608, 613
MboII GAAGA 6 cut(s) 43, 55, 683, 766, 863, 897
MflI RGATCY 2 cut(s) 266, 613
MhlI GDGCHC 4 cut(s) 189, 332, 661, 840
MluCI AATT 5 cut(s) 100, 126, 540, 635, 854
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 78
MseI TTAA 3 cut(s) 285, 563, 789
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 2 cut(s) 79, 194
Mva1269I GAATGC 1 cut(s) 361
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeII GATC 6 cut(s) 160, 266, 376, 396, 608, 613
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 1 cut(s) 35
NmuCI GTSAC 2 cut(s) 234, 719
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 131
PaeR7I CTCGAG 2 cut(s) 412, 919
PciSI GCTCTTC 1 cut(s) 56
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 119, 343
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 5 cut(s) 215, 292, 499, 502, 917
PleI GAGTC 1 cut(s) 83
PmaCI CACGTG 1 cut(s) 656
PmlI CACGTG 1 cut(s) 656
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 840
PspCI CACGTG 1 cut(s) 656
PspEI GGTNACC 2 cut(s) 598, 719
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 3 cut(s) 147, 191, 642
PspXI VCTCGAGB 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 266, 613
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 158, 338
RsaNI GTAC 2 cut(s) 157, 337
Rsr2I CGGWCCG 1 cut(s) 191
RsrII CGGWCCG 1 cut(s) 191
SacI GAGCTC 1 cut(s) 840
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 3 cut(s) 285, 563, 789
SatI GCNGC 5 cut(s) 214, 291, 498, 501, 916
Sau3AI GATC 6 cut(s) 160, 266, 376, 396, 608, 613
Sau96I GGNCC 3 cut(s) 147, 191, 642
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 4 cut(s) 189, 332, 661, 840
SfaNI GCATC 3 cut(s) 13, 457, 740
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 2 cut(s) 412, 919
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 3 cut(s) 147, 191, 642
SlaI CTCGAG 2 cut(s) 412, 919
SmlI CTYRAG 3 cut(s) 284, 412, 919
SmoI CTYRAG 3 cut(s) 284, 412, 919
Sse9I AATT 5 cut(s) 100, 126, 540, 635, 854
SsiI CCGC 4 cut(s) 142, 150, 213, 645
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 429, 872
SstI GAGCTC 1 cut(s) 840
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 4 cut(s) 19, 156, 336, 742
TaiI ACGT 2 cut(s) 658, 864
TaqI TCGA 7 cut(s) 131, 341, 375, 413, 628, 849, 920
TasI AATT 5 cut(s) 100, 126, 540, 635, 854
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 2 cut(s) 119, 343
Tru1I TTAA 3 cut(s) 285, 563, 789
Tru9I TTAA 3 cut(s) 285, 563, 789
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 2 cut(s) 234, 719
TseI GCWGC 4 cut(s) 290, 497, 500, 915
Tsp45I GTSAC 2 cut(s) 234, 719
TspDTI ATGAA 6 cut(s) 16, 405, 428, 683, 690, 905
TspGWI ACGGA 2 cut(s) 204, 897
TspRI CASTG 1 cut(s) 159
Vha464I CTTAAG 1 cut(s) 284
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 3 cut(s) 147, 191, 642
XapI RAATTY 1 cut(s) 854
XhoI CTCGAG 2 cut(s) 412, 919
XspI CTAG 2 cut(s) 429, 872
ZrmI AGTACT 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.