pycom14g16450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009028
Physical Location & Seq
Reverse (-)
18719377 .. 18722208
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16450.6

Sequence Viewer

Length: 933 bp
ATGAATCGCTTCAAGACCGTAGCTTTCATCTGGCTCCTCTATGCCTTGTTTTTCTACTCTCTCTTCCGAATGGCCCTCAGGAACTCCGACTCCTCACGCAATTACTCCTCCGCCTCCGATTCAGTAATTTCGAACAAAGAGCGGAGGTCCATATTATACGATCGGATGGGTCGGGATTTGGAAGACCATGGACCAGTTTTTCTCAACCGCGGCGGCCGAACCTCTCAGTCTCTGTCACTTTCTGATATTTTTACGATAAGGGATGGATCTGTAGCACCTGTGCTTAAGGCAGCAAATCCTCCTGTTCGTGCTAATGTGTTGTATCTGAGCACGCAGTACTCGCTTCCGATTTCGAAGGCACTCAAACCTTTACTCGATCGATCTTACTTCGATAAGGCAATCTGGTTTCAGAACTCGAGCTTGTATCATTTTAGTATGTTTCATGCCTCGCATCATATTTCACCTGTTCCTGCTACGCAAGGCGAGATTGAAACCGAAGCAGCTGCCGTTAGGACTACTTCACTGGGTCTCTGCCCTTTAAAAATTGTCTTAGACAGAGTTGTCTTGACCTCAACAGGTGTGCTTCTTGGGTGCTGGCAGGTAATCTCTGGATCAGATCCTATAACCATTCGAGCAAAATTGAGGACCGCGCTTCCACGTGCACCAGAAAAGCAACTTTACGATGCTGCCATTCTTCATACTTCATTTGCAAGGCTTCTTGGTCACCCAAAAGCATCATCCAAGGTGCTATCTAATACTTCAGATCAAATCCACTTCTTCCAAGAGCTTGTTCATCGTCTAAACAACGAACTCCAAGGATTTGAGGCAGTGGTATCTGAGCTCTGGTATGTGGAGGAATTCGACGTGTTGGCTCTAGCATTGAACGGAAGAATGAAGGCGCGCAGGTTCCAACTTGGCTGCTCAAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.86

Weight (kDa)

9.62

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 560
Acc36I ACCTGC 2 cut(s) 589, 894
AccBSI CCGCTC 1 cut(s) 142
AccII CGCG 3 cut(s) 210, 650, 901
AciI CCGC 6 cut(s) 111, 142, 208, 210, 213, 648
AclWI GGATC 3 cut(s) 274, 611, 619
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 857
AcuI CTGAAG 1 cut(s) 744
AcvI CACGTG 1 cut(s) 659
AfaI GTAC 1 cut(s) 338
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 864
AgsI TTSAA 3 cut(s) 13, 491, 883
AjiI CACGTC 1 cut(s) 865
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 5 cut(s) 23, 420, 503, 787, 841
AluI AGCT 5 cut(s) 23, 420, 503, 787, 841
Alw21I GWGCWC 3 cut(s) 332, 664, 843
Alw26I GTCTC 2 cut(s) 234, 533
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 3 cut(s) 274, 611, 619
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 415
AoxI GGCC 2 cut(s) 72, 214
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 5 cut(s) 290, 500, 503, 686, 918
ApoI RAATTY 1 cut(s) 857
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 3 cut(s) 652, 901, 903
AspS9I GGNCC 4 cut(s) 73, 147, 191, 645
AsuHPI GGTGA 2 cut(s) 453, 716
AsuII TTCGAA 2 cut(s) 131, 353
AvaI CYCGRG 1 cut(s) 415
AvaII GGWCC 3 cut(s) 147, 191, 645
AxyI CCTNAGG 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 664
BanII GRGCYC 1 cut(s) 843
BbrPI CACGTG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 3 cut(s) 332, 664, 843
BbvI GCAGC 5 cut(s) 302, 490, 512, 673, 905
BccI CCATC 2 cut(s) 160, 257
BceAI ACGGC 1 cut(s) 491
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BcoDI GTCTC 2 cut(s) 234, 533
BfaI CTAG 1 cut(s) 875
BfmI CTRYAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 284
BfuAI ACCTGC 2 cut(s) 589, 894
BisI GCNGC 7 cut(s) 211, 214, 291, 501, 504, 687, 919
BlsI GCNGC 7 cut(s) 212, 215, 292, 502, 505, 688, 920
BmcAI AGTACT 1 cut(s) 338
Bme18I GGWCC 3 cut(s) 147, 191, 645
BmeT110I CYCGRG 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 4 cut(s) 73, 147, 191, 645
BmiI GGNNCC 2 cut(s) 35, 908
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 3 cut(s) 460, 673, 743
BmuI ACTGGG 1 cut(s) 533
BpiI GAAGAC 1 cut(s) 189
Bpu14I TTCGAA 2 cut(s) 131, 353
BpuEI CTTGAG 1 cut(s) 907
Bsa29I ATCGAT 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 4 cut(s) 187, 208, 741, 814
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse1I ACTGG 2 cut(s) 194, 528
Bse21I CCTNAGG 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 379
BseDI CCNNGG 4 cut(s) 187, 208, 741, 814
BseGI GGATG 3 cut(s) 171, 268, 737
BseMII CTCAG 4 cut(s) 91, 239, 317, 828
BseNI ACTGG 2 cut(s) 194, 528
BsePI GCGCGC 1 cut(s) 899
BseRI GAGGAG 3 cut(s) 26, 82, 97
BseSI GKGCMC 1 cut(s) 664
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 5 cut(s) 302, 490, 512, 673, 905
Bsh1236I CGCG 3 cut(s) 210, 650, 901
Bsh1285I CGRYCG 3 cut(s) 163, 217, 379
BshFI GGCC 2 cut(s) 74, 216
BshVI ATCGAT 1 cut(s) 379
BsiEI CGRYCG 3 cut(s) 163, 217, 379
BsiHKAI GWGCWC 3 cut(s) 332, 664, 843
BsiHKCI CYCGRG 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 234, 533
BsnI GGCC 2 cut(s) 74, 216
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 1 cut(s) 415
Bsp119I TTCGAA 2 cut(s) 131, 353
Bsp1286I GDGCHC 3 cut(s) 332, 664, 843
Bsp143I GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
Bsp19I CCATGG 1 cut(s) 187
BspACI CCGC 6 cut(s) 111, 142, 208, 210, 213, 648
BspANI GGCC 2 cut(s) 74, 216
BspCNI CTCAG 4 cut(s) 90, 238, 318, 829
BspDI ATCGAT 1 cut(s) 379
BspFNI CGCG 3 cut(s) 210, 650, 901
BspLI GGNNCC 2 cut(s) 35, 908
BspMI ACCTGC 2 cut(s) 589, 894
BspPI GGATC 3 cut(s) 274, 611, 619
BspT104I TTCGAA 2 cut(s) 131, 353
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 533
BsrBI CCGCTC 1 cut(s) 142
BsrI ACTGG 2 cut(s) 194, 528
BssECI CCNNGG 4 cut(s) 187, 208, 741, 814
BssHII GCGCGC 1 cut(s) 899
BssMI GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
BssT1I CCWWGG 3 cut(s) 187, 741, 814
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 659
BstBI TTCGAA 2 cut(s) 131, 353
BstC8I GCNNGC 3 cut(s) 332, 596, 901
BstDEI CTNAG 5 cut(s) 77, 225, 326, 550, 837
BstDSI CCRYGG 2 cut(s) 187, 208
BstEII GGTNACC 1 cut(s) 722
BstF5I GGATG 3 cut(s) 171, 268, 737
BstFNI CGCG 3 cut(s) 210, 650, 901
BstHHI GCGC 3 cut(s) 652, 901, 903
BstKTI GATC 7 cut(s) 163, 269, 379, 383, 614, 619, 766
BstMAI GTCTC 2 cut(s) 234, 533
BstMBI GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
BstMCI CGRYCG 3 cut(s) 163, 217, 379
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstPI GGTNACC 1 cut(s) 722
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 664
BstUI CGCG 3 cut(s) 210, 650, 901
BstV1I GCAGC 5 cut(s) 302, 490, 512, 673, 905
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 266, 616
BstYI RGATCY 2 cut(s) 266, 616
BstZI CGGCCG 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 379
Bsu36I CCTNAGG 1 cut(s) 77
BsuRI GGCC 2 cut(s) 74, 216
BsuTUI ATCGAT 1 cut(s) 379
BtgI CCRYGG 2 cut(s) 187, 208
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 3 cut(s) 171, 268, 737
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 521, 834
BveI ACCTGC 2 cut(s) 589, 894
Cac8I GCNNGC 3 cut(s) 332, 596, 901
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 3 cut(s) 652, 901, 903
Cfr13I GGNCC 4 cut(s) 73, 147, 191, 645
Cfr42I CCGCGG 1 cut(s) 211
ClaI ATCGAT 1 cut(s) 379
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 188, 443
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 5 cut(s) 77, 225, 326, 550, 837
DpnI GATC 7 cut(s) 162, 268, 378, 382, 613, 618, 765
DpnII GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
DraI TTTAAA 1 cut(s) 540
DrdI GACNNNNNNGTC 1 cut(s) 560
DseDI GACNNNNNNGTC 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 841
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 3 cut(s) 187, 741, 814
Eco24I GRGCYC 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 533
Eco47I GGWCC 3 cut(s) 147, 191, 645
Eco52I CGGCCG 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 841
Eco57I CTGAAG 1 cut(s) 744
Eco72I CACGTG 1 cut(s) 659
Eco81I CCTNAGG 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 415
Eco91I GGTNACC 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 841
EcoO65I GGTNACC 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 857
EcoT14I CCWWGG 3 cut(s) 187, 741, 814
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 3 cut(s) 187, 741, 814
FaeI CATG 2 cut(s) 191, 446
FatI CATG 2 cut(s) 187, 442
Fnu4HI GCNGC 7 cut(s) 211, 214, 291, 501, 504, 687, 919
FokI GGATG 3 cut(s) 178, 275, 724
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 7 cut(s) 211, 214, 291, 501, 504, 687, 919
FspBI CTAG 1 cut(s) 875
GlaI GCGC 3 cut(s) 651, 900, 902
GluI GCNGC 7 cut(s) 211, 214, 291, 501, 504, 687, 919
HaeIII GGCC 2 cut(s) 74, 216
HhaI GCGC 3 cut(s) 652, 901, 903
Hin1II CATG 2 cut(s) 191, 446
Hin6I GCGC 3 cut(s) 650, 899, 901
HinP1I GCGC 3 cut(s) 650, 899, 901
HinfI GANTC 3 cut(s) 4, 89, 119
HphI GGTGA 2 cut(s) 453, 716
Hpy166II GTNNAC 1 cut(s) 662
Hpy188III TCNNGA 6 cut(s) 13, 79, 173, 565, 609, 924
Hpy8I GTNNAC 1 cut(s) 662
Hpy99I CGWCG 1 cut(s) 866
HpyAV CCTTC 2 cut(s) 349, 889
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 2 cut(s) 658, 864
HpyCH4V TGCA 2 cut(s) 662, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 5 cut(s) 77, 225, 326, 550, 837
HpySE526I ACGT 2 cut(s) 658, 864
Hsp92II CATG 2 cut(s) 191, 446
HspAI GCGC 3 cut(s) 650, 899, 901
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
LmnI GCTCC 1 cut(s) 39
Lsp1109I GCAGC 5 cut(s) 302, 490, 512, 673, 905
LweI GCATC 3 cut(s) 460, 673, 743
MaeI CTAG 1 cut(s) 875
MaeII ACGT 2 cut(s) 658, 864
MaeIII GTNAC 2 cut(s) 234, 722
MalI GATC 7 cut(s) 162, 268, 378, 382, 613, 618, 765
MbiI CCGCTC 1 cut(s) 142
MboI GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
MboII GAAGA 5 cut(s) 55, 194, 686, 769, 900
MflI RGATCY 2 cut(s) 266, 616
MhlI GDGCHC 3 cut(s) 332, 664, 843
MluCI AATT 5 cut(s) 100, 126, 543, 638, 857
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 285, 539
MspA1I CMGCKG 2 cut(s) 210, 503
MspCI CTTAAG 1 cut(s) 284
MvnI CGCG 3 cut(s) 210, 650, 901
MwoI GCNNNNNNNGC 1 cut(s) 340
NcoI CCATGG 1 cut(s) 187
NdeII GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
NlaIII CATG 2 cut(s) 191, 446
NlaIV GGNNCC 2 cut(s) 35, 908
NmuCI GTSAC 2 cut(s) 234, 722
NspV TTCGAA 2 cut(s) 131, 353
PaeR7I CTCGAG 1 cut(s) 415
PauI GCGCGC 1 cut(s) 899
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 4, 119
PkrI GCNGC 7 cut(s) 212, 215, 292, 502, 505, 688, 920
Ple19I CGATCG 2 cut(s) 163, 379
PleI GAGTC 1 cut(s) 83
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 843
PspCI CACGTG 1 cut(s) 659
PspEI GGTNACC 1 cut(s) 722
PspN4I GGNNCC 2 cut(s) 35, 908
PspPI GGNCC 4 cut(s) 73, 147, 191, 645
PspXI VCTCGAGB 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 266, 616
PteI GCGCGC 1 cut(s) 899
PvuI CGATCG 2 cut(s) 163, 379
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SacI GAGCTC 1 cut(s) 843
SacII CCGCGG 1 cut(s) 211
SaqAI TTAA 2 cut(s) 285, 539
SatI GCNGC 7 cut(s) 211, 214, 291, 501, 504, 687, 919
Sau3AI GATC 7 cut(s) 160, 266, 376, 380, 611, 616, 763
Sau96I GGNCC 4 cut(s) 73, 147, 191, 645
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 3 cut(s) 332, 664, 843
SfaNI GCATC 3 cut(s) 460, 673, 743
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 415
Sfr303I CCGCGG 1 cut(s) 211
SfuI TTCGAA 2 cut(s) 131, 353
SgrBI CCGCGG 1 cut(s) 211
SinI GGWCC 3 cut(s) 147, 191, 645
SlaI CTCGAG 1 cut(s) 415
SmlI CTYRAG 3 cut(s) 284, 415, 922
SmoI CTYRAG 3 cut(s) 284, 415, 922
Sse9I AATT 5 cut(s) 100, 126, 543, 638, 857
SsiI CCGC 6 cut(s) 111, 142, 208, 210, 213, 648
SspMI CTAG 1 cut(s) 875
SstI GAGCTC 1 cut(s) 843
StyI CCWWGG 3 cut(s) 187, 741, 814
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 2 cut(s) 661, 867
TaqI TCGA 8 cut(s) 131, 353, 375, 379, 390, 416, 631, 861
TasI AATT 5 cut(s) 100, 126, 543, 638, 857
TatI WGTACW 1 cut(s) 336
TauI GCSGC 2 cut(s) 213, 216
TfiI GAWTC 2 cut(s) 4, 119
Tru1I TTAA 2 cut(s) 285, 539
Tru9I TTAA 2 cut(s) 285, 539
TscAI CASTG 2 cut(s) 528, 834
TseFI GTSAC 2 cut(s) 234, 722
TseI GCWGC 5 cut(s) 290, 500, 503, 686, 918
Tsp45I GTSAC 2 cut(s) 234, 722
TspDTI ATGAA 7 cut(s) 16, 17, 431, 686, 693, 782, 908
TspGWI ACGGA 1 cut(s) 900
TspRI CASTG 2 cut(s) 528, 834
Vha464I CTTAAG 1 cut(s) 284
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 3 cut(s) 147, 191, 645
XapI RAATTY 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 875
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.