MD14G1198100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009028
Physical Location & Seq
Reverse (-)
28811460 .. 28814692
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1198100.v1.1.491

Sequence Viewer

Length: 930 bp
ATGAATCGCCCCAAGACCGTAGCTTTCATCTGGCTCCTCTATGCCTTGTTTTTCTACTCACTCTTCCGAGTGGCCCTCAGGAACTCAGACTCCTCACGCAATTACTCCTCCGCCTCCGATGCTGTGATTTCGAATAAGGACCGGAGGTCTGCATTATACGATCAGATGGGTCGGGATTTGAAAGACCACGGACCGGTTTTTCTCAACCGCGGCGGCCGGACCTCTCAGTCTCTGTCACTTTCTGATATTTTTACGATAAGGGATGGATCTGTAGCACCTGTACTTAAGGCAGCAAATCCTCCTGTTCGTGCTAATGTGTTGTATCTGAGCACGCAGTACTCGCTTCCGATTTCGAAGGCGCTCAAACCTATTCTCGATCCTTACTTCGATAAGGCAATCTGGTTTCAGAACTCGAGCTTGTATCATTTTAGTATGTTTCATGCCTCGCATCATATTTCACCCGTTCCTGCTACTCAAGACGAGATTGAAACCGAAGTAGCTGCCGTTAGGACTATTGCACAGGGTCTCTGCCCTTTAAAAATTGTCTTGGACAGAGTGGTCTTGACCTCAACAGGTGTGCTTCTTGGGTGCTGGCAGGTAACCTCTGGATCAGATCCTATAACCATTCGAGCAAAATTGAGGACCGCACTTCCACATGCACCAGAAAAGCAACTTTATGATGCTGCGATTCTTCATACTTCATTTACAAGGCTTCTTGGTCACCCAAAAGCATCATCCAAGGTGCTATCTAATACTTCAGATCAAATCCACTTCTTCCAAGAGCTTGTTCATCGTCTAAACAACGAACTCCAAGGATTTGAGGCAGTGGTATCTGAACTCTGGTATGTGGAGGAATTTGACGTTTTGGCTCTAGCATTGAACGGAAGAATGAAGGCGCGCAGGTTCCAACTTGGCTGCTCAAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.63

Weight (kDa)

9.42

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 226, 557
Acc36I ACCTGC 2 cut(s) 586, 891
AccII CGCG 2 cut(s) 210, 898
AciI CCGC 5 cut(s) 111, 208, 210, 213, 645
AclWI GGATC 4 cut(s) 274, 371, 608, 616
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 854
AcuI CTGAAG 1 cut(s) 741
AfaI GTAC 2 cut(s) 282, 338
AfiI CCNNNNNNNGG 1 cut(s) 193
AflII CTTAAG 1 cut(s) 284
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 3 cut(s) 181, 488, 880
AhdI GACNNNNNGTC 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 4 cut(s) 23, 417, 500, 784
AluI AGCT 4 cut(s) 23, 417, 500, 784
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 2 cut(s) 234, 530
AlwI GGATC 4 cut(s) 274, 371, 608, 616
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 412
AoxI GGCC 2 cut(s) 72, 214
ApeKI GCWGC 4 cut(s) 290, 500, 683, 915
ApoI RAATTY 1 cut(s) 854
AsiGI ACCGGT 1 cut(s) 193
AspLEI GCGC 3 cut(s) 361, 898, 900
AspS9I GGNCC 5 cut(s) 73, 139, 191, 219, 642
AsuHPI GGTGA 2 cut(s) 450, 713
AsuII TTCGAA 2 cut(s) 131, 353
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 4 cut(s) 139, 191, 219, 642
AxyI CCTNAGG 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 332
BbvI GCAGC 4 cut(s) 302, 487, 670, 902
BccI CCATC 2 cut(s) 160, 257
BceAI ACGGC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BcoDI GTCTC 2 cut(s) 234, 530
BfaI CTAG 1 cut(s) 872
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 362
BfrI CTTAAG 1 cut(s) 284
BfuAI ACCTGC 2 cut(s) 586, 891
BisI GCNGC 6 cut(s) 211, 214, 291, 501, 684, 916
BlsI GCNGC 6 cut(s) 212, 215, 292, 502, 685, 917
BmcAI AGTACT 1 cut(s) 338
Bme18I GGWCC 4 cut(s) 139, 191, 219, 642
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 412
BmgT120I GGNCC 5 cut(s) 73, 139, 191, 219, 642
BmiI GGNNCC 2 cut(s) 35, 905
BmsI GCATC 4 cut(s) 109, 457, 670, 740
BplI GAGNNNNNCTC 2 cut(s) 60, 92
Bpu14I TTCGAA 2 cut(s) 131, 353
BpuEI CTTGAG 2 cut(s) 459, 904
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 4 cut(s) 187, 208, 738, 811
BsaWI WCCGGW 2 cut(s) 141, 193
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 1 cut(s) 193
Bse21I CCTNAGG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 187, 208, 738, 811
BseGI GGATG 2 cut(s) 268, 734
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 4 cut(s) 91, 99, 239, 317
BsePI GCGCGC 1 cut(s) 896
BseRI GAGGAG 3 cut(s) 26, 82, 97
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 4 cut(s) 302, 487, 670, 902
Bsh1236I CGCG 2 cut(s) 210, 898
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 74, 216
BshTI ACCGGT 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 3 cut(s) 142, 194, 217
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 234, 530
BsnI GGCC 2 cut(s) 74, 216
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 1 cut(s) 412
Bsp119I TTCGAA 2 cut(s) 131, 353
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 6 cut(s) 160, 266, 376, 608, 613, 760
BspACI CCGC 5 cut(s) 111, 208, 210, 213, 645
BspANI GGCC 2 cut(s) 74, 216
BspCNI CTCAG 4 cut(s) 90, 98, 238, 318
BspFNI CGCG 2 cut(s) 210, 898
BspLI GGNNCC 2 cut(s) 35, 905
BspMI ACCTGC 2 cut(s) 586, 891
BspPI GGATC 4 cut(s) 274, 371, 608, 616
BspT104I TTCGAA 2 cut(s) 131, 353
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 530
BsrFI RCCGGY 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 4 cut(s) 187, 208, 738, 811
BssHII GCGCGC 1 cut(s) 896
BssMI GATC 6 cut(s) 160, 266, 376, 608, 613, 760
BssT1I CCWWGG 2 cut(s) 738, 811
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 284
BstBI TTCGAA 2 cut(s) 131, 353
BstC8I GCNNGC 3 cut(s) 332, 593, 898
BstDEI CTNAG 4 cut(s) 77, 85, 225, 326
BstDSI CCRYGG 2 cut(s) 187, 208
BstEII GGTNACC 2 cut(s) 598, 719
BstF5I GGATG 2 cut(s) 268, 734
BstFNI CGCG 2 cut(s) 210, 898
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 3 cut(s) 361, 898, 900
BstKTI GATC 6 cut(s) 163, 269, 379, 611, 616, 763
BstMAI GTCTC 2 cut(s) 234, 530
BstMBI GATC 6 cut(s) 160, 266, 376, 608, 613, 760
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 119, 340
BstNSI RCATGY 1 cut(s) 659
BstPI GGTNACC 2 cut(s) 598, 719
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 2 cut(s) 210, 898
BstV1I GCAGC 4 cut(s) 302, 487, 670, 902
BstX2I RGATCY 2 cut(s) 266, 613
BstYI RGATCY 2 cut(s) 266, 613
BstZI CGGCCG 1 cut(s) 214
Bsu36I CCTNAGG 1 cut(s) 77
BsuRI GGCC 2 cut(s) 74, 216
BtgI CCRYGG 2 cut(s) 187, 208
BtsCI GGATG 2 cut(s) 268, 734
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 1 cut(s) 831
BveI ACCTGC 2 cut(s) 586, 891
Cac8I GCNNGC 3 cut(s) 332, 593, 898
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 3 cut(s) 361, 898, 900
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 5 cut(s) 73, 139, 191, 219, 642
Cfr42I CCGCGG 1 cut(s) 211
CpoI CGGWCCG 1 cut(s) 191
Csp6I GTAC 2 cut(s) 281, 337
CspAI ACCGGT 1 cut(s) 193
CspI CGGWCCG 1 cut(s) 191
CviAII CATG 2 cut(s) 440, 656
CviQI GTAC 2 cut(s) 281, 337
DdeI CTNAG 4 cut(s) 77, 85, 225, 326
DpnI GATC 6 cut(s) 162, 268, 378, 610, 615, 762
DpnII GATC 6 cut(s) 160, 266, 376, 608, 613, 760
DraI TTTAAA 1 cut(s) 537
DrdI GACNNNNNNGTC 2 cut(s) 226, 557
DriI GACNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 2 cut(s) 226, 557
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 738, 811
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 4 cut(s) 139, 191, 219, 642
Eco52I CGGCCG 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 741
Eco81I CCTNAGG 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 412
Eco91I GGTNACC 2 cut(s) 598, 719
EcoO65I GGTNACC 2 cut(s) 598, 719
EcoT14I CCWWGG 2 cut(s) 738, 811
ErhI CCWWGG 2 cut(s) 738, 811
FaeI CATG 2 cut(s) 443, 659
FatI CATG 2 cut(s) 439, 655
Fnu4HI GCNGC 6 cut(s) 211, 214, 291, 501, 684, 916
FokI GGATG 2 cut(s) 275, 721
Fsp4HI GCNGC 6 cut(s) 211, 214, 291, 501, 684, 916
FspBI CTAG 1 cut(s) 872
GlaI GCGC 3 cut(s) 360, 897, 899
GluI GCNGC 6 cut(s) 211, 214, 291, 501, 684, 916
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 2 cut(s) 74, 216
HapII CCGG 3 cut(s) 142, 194, 217
HhaI GCGC 3 cut(s) 361, 898, 900
Hin1II CATG 2 cut(s) 443, 659
Hin6I GCGC 3 cut(s) 359, 896, 898
HinP1I GCGC 3 cut(s) 359, 896, 898
HinfI GANTC 3 cut(s) 4, 89, 688
HpaII CCGG 3 cut(s) 142, 194, 217
HphI GGTGA 2 cut(s) 450, 713
Hpy188III TCNNGA 7 cut(s) 79, 173, 374, 476, 562, 606, 921
HpyAV CCTTC 2 cut(s) 349, 886
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 3 cut(s) 152, 518, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 340
HpyF3I CTNAG 4 cut(s) 77, 85, 225, 326
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 2 cut(s) 443, 659
HspAI GCGC 3 cut(s) 359, 896, 898
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 6 cut(s) 160, 266, 376, 608, 613, 760
LmnI GCTCC 1 cut(s) 39
Lsp1109I GCAGC 4 cut(s) 302, 487, 670, 902
LweI GCATC 4 cut(s) 109, 457, 670, 740
MaeI CTAG 1 cut(s) 872
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 3 cut(s) 234, 598, 719
MalI GATC 6 cut(s) 162, 268, 378, 610, 615, 762
MboI GATC 6 cut(s) 160, 266, 376, 608, 613, 760
MboII GAAGA 4 cut(s) 55, 683, 766, 897
MflI RGATCY 2 cut(s) 266, 613
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 4 cut(s) 100, 540, 635, 854
MlyI GAGTC 1 cut(s) 83
MseI TTAA 2 cut(s) 285, 536
MspA1I CMGCKG 1 cut(s) 210
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 3 cut(s) 142, 194, 217
MvnI CGCG 2 cut(s) 210, 898
MwoI GCNNNNNNNGC 2 cut(s) 119, 340
NdeII GATC 6 cut(s) 160, 266, 376, 608, 613, 760
NlaIII CATG 2 cut(s) 443, 659
NlaIV GGNNCC 2 cut(s) 35, 905
NmuCI GTSAC 2 cut(s) 234, 719
NspI RCATGY 1 cut(s) 659
NspV TTCGAA 2 cut(s) 131, 353
PaeR7I CTCGAG 1 cut(s) 412
PauI GCGCGC 1 cut(s) 896
PfeI GAWTC 2 cut(s) 4, 688
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 6 cut(s) 212, 215, 292, 502, 685, 917
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PspEI GGTNACC 2 cut(s) 598, 719
PspN4I GGNNCC 2 cut(s) 35, 905
PspPI GGNCC 5 cut(s) 73, 139, 191, 219, 642
PspXI VCTCGAGB 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 266, 613
PteI GCGCGC 1 cut(s) 896
RsaI GTAC 2 cut(s) 282, 338
RsaNI GTAC 2 cut(s) 281, 337
Rsr2I CGGWCCG 1 cut(s) 191
RsrII CGGWCCG 1 cut(s) 191
SacII CCGCGG 1 cut(s) 211
SaqAI TTAA 2 cut(s) 285, 536
SatI GCNGC 6 cut(s) 211, 214, 291, 501, 684, 916
Sau3AI GATC 6 cut(s) 160, 266, 376, 608, 613, 760
Sau96I GGNCC 5 cut(s) 73, 139, 191, 219, 642
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 4 cut(s) 109, 457, 670, 740
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 412
Sfr303I CCGCGG 1 cut(s) 211
SfuI TTCGAA 2 cut(s) 131, 353
SgrBI CCGCGG 1 cut(s) 211
SinI GGWCC 4 cut(s) 139, 191, 219, 642
SlaI CTCGAG 1 cut(s) 412
SmlI CTYRAG 4 cut(s) 284, 412, 474, 919
SmoI CTYRAG 4 cut(s) 284, 412, 474, 919
Sse9I AATT 4 cut(s) 100, 540, 635, 854
SsiI CCGC 5 cut(s) 111, 208, 210, 213, 645
SspMI CTAG 1 cut(s) 872
StyI CCWWGG 2 cut(s) 738, 811
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 864
TaqI TCGA 6 cut(s) 131, 353, 375, 387, 413, 628
TasI AATT 4 cut(s) 100, 540, 635, 854
TatI WGTACW 2 cut(s) 280, 336
TauI GCSGC 2 cut(s) 213, 216
TfiI GAWTC 2 cut(s) 4, 688
Tru1I TTAA 2 cut(s) 285, 536
Tru9I TTAA 2 cut(s) 285, 536
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 2 cut(s) 234, 719
TseI GCWGC 4 cut(s) 290, 500, 683, 915
Tsp45I GTSAC 2 cut(s) 234, 719
TspDTI ATGAA 7 cut(s) 16, 17, 428, 683, 690, 779, 905
TspGWI ACGGA 2 cut(s) 204, 897
TspRI CASTG 1 cut(s) 831
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 4 cut(s) 139, 191, 219, 642
XapI RAATTY 1 cut(s) 854
XceI RCATGY 1 cut(s) 659
XhoI CTCGAG 1 cut(s) 412
XspI CTAG 1 cut(s) 872
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.