Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004941

Physical Location & Seq

Genomic Coordinates Reverse (-)

35723414 .. 35727104

Gene Structure 3691 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1226700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGCTGCTTTTGCTAATGGGGAAGAGCGGGAGGTGCAGAAGAACTACTGGGTGGAGCACTCCGCCGATTTGACCGTTGAGGCCATGATGCTCGACTCCAAGGCCTCAGATCTCGACAAGGAAGAACGACCTGAGGTGCTTTCGTTGCTCCCTCCCTATGAGGGCAAATCGGTTGTAGAACTCGGAGCTGGGATTGGTCGTTTCACTGGAGAATTAGCTGAAAAGGCCGGTCAGCTTTTCGCATTGGACTTCATTGACAGTGTGATAAAGAAGAATGAAAGCATTAATGGACACCATAAGAACGTCAAGTTCATGTGCGCTGATGTGACATCACCTGATCTGAAGATTTCTGAAAACTCGGTGGATTTGGTATTCTCAAATTGGCTTCTTATGTATCTGTCAGATACGGAGGTAGAGAATTTGGTGGAAAGGATGATGGGATGGTTGAAGGTCGGTGGACATATCTTTTTCAGAGAATCTTGTTTCCACCAATCTGGAGACTCCAAACGAAAATCCAACCCAACCCATTACAGGGAGCCAAGATTTTATACCAAGGTTTTCAAAGACTGCCACATGCGTGATGATTCCGGGAATTTCTTTGAACTCTCTCTTGTTAGTTGCAAATGTATTGGAGCTTATGTTCGGAACAAAAGGAATCAAAATCAGATCTGCTGGTTATGGCAGAAAGTCCCTTCAGAAAATGATAGAGGGTTCCAGCAGTTCTTAGATAATGTTCAGTATAAAAATACTGGCATACTACGATATGAGCGTGTGTTTGGGCGAGGTTTCGTGAGCACAGGAGGGATCGAAACTACTAAAGAATTTGTGGCAAAGTTGGATCTGAAGCCTGGCCAGAAAGTTCTCGATGTAGGCTGTGGCATCGGGGGAGGTGACTTTTATATGGCCTCAAACTTTGATGTTGAGGTTATTGGAATTGACCTCTCCGTGAATATGATTTCCTTTGCTCTCGAACAAGCTATTGGACTCAAATGCGCTGTCGAGTTTGAAGTTGCTGATTGCACAAAAAAGACTTATCCTGACAATACCTTTGATGTGATATACAGTCGCGACACCATTTTACACATTCAAGACAAGCCTGCATTGTTCCGATCCTTCTACAAGTGGTTGAAGCCAGGCGGCAAAGTTCTCATCAGTGATTACTGTAGGTGTGCTGGAACTCCATCAGAAAACTTTGCTGACTATATTAAGCAAAGAGGATATGATCTCCATGATGTAAAAGCTTATGGTCAGATGCTCAAGGATGCTGGCTTTGATGAGGTCATTGCTGAGGATCGTACTGATCAGTTCAAAGCTGTTTTGGAGCGGGAACTGGCTTCCGTTGAGAAGGATAAGGATTCATTCGTCCAGGACTTTTCTCAAGAGGATTATGATGACATCGTTGGTGGATGGAAGGCAAAGCTGATCAGGGTTGATTCCGGCGAGCAGAAGTGGGGCCTGTTCATTGCCAAGAAGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001505 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006576 GO:0006629 GO:0006644 GO:0006650 GO:0006656 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008610 GO:0008654 GO:0008757 GO:0009058 GO:0009308 GO:0009309 GO:0009555 GO:0009605 GO:0009653 GO:0009791 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010183 GO:0016043 GO:0016049 GO:0016740 GO:0016741 GO:0019637 GO:0019695 GO:0022414 GO:0022622 GO:0030154 GO:0032259 GO:0032501 GO:0032502 GO:0032989 GO:0034641 GO:0040007 GO:0040011 GO:0042133 GO:0042136 GO:0042221 GO:0042330 GO:0042401 GO:0042425 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045017 GO:0046470 GO:0046474 GO:0046486 GO:0048229 GO:0048364 GO:0048468 GO:0048528 GO:0048588 GO:0048589 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050896 GO:0050918 GO:0051704 GO:0052667 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090407 GO:0090696 GO:0097164 GO:0099402 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 map00564

Pfam Domains

Protein Families

Methyltransf_11 Methyltransf_25 Methyltransf_31

Protein Analysis

493

Amino Acids

55.93

Weight (kDa)

5.3

Isoelectric Point (pI)

29.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_31	PF13847	55 - 178	6e-09	Methyltransferase domain
Methyltransf_PK	PF05891	56 - 192	5.1e-08	AdoMet dependent proline di-methyltransferase
Methyltransf_25	PF13649	58 - 153	2.2e-12	Methyltransferase domain
Methyltransf_12	PF08242	59 - 155	1.5e-07	Methyltransferase domain
Methyltransf_11	PF08241	60 - 157	1.2e-12	Methyltransferase domain
Ubie_methyltran	PF01209	273 - 390	2.8e-10	ubiE/COQ5 methyltransferase family
CMAS	PF02353	273 - 387	1.3e-13	Mycolic acid cyclopropane synthetase
Methyltransf_23	PF13489	277 - 425	3.1e-19	Methyltransferase domain
Methyltransf_31	PF13847	282 - 389	8.8e-22	Methyltransferase domain
Methyltransf_25	PF13649	287 - 380	3.7e-19	Methyltransferase domain
Methyltransf_11	PF08241	288 - 384	1.6e-19	Methyltransferase domain
Methyltransf_12	PF08242	288 - 381	3e-13	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48600 AT1G73600 AT1G73600 AT3G18000
fragaria_vesca	FvH4_5g17300 FvH4_5g17300 FvH4_5g17300 FvH4_5g17300
malus_domestica	MD06G1226700.v1.1 MD14G1236000.v1.1
prunus_persica	Prupe.5G233000_v2.0.a1 Prupe.5G233000_v2.0.a1
pyrus_communis	pycom06g20300 pycom14g19710
rosa_chinensis	RchiOBHm_Chr3g0488691 RchiOBHm_Chr7g0178471
rosa_laevigata	RLG00000005444
rosa_multiflora	Rmu_sc0001208.1_g000086 Rmu_sc0010235.1_g000004
rosa_roxburghii	Rroxscaffold_3G00274870 Rroxscaffold_6G00393940
rosa_rugosa	Rorug03G0240800 Rorug06G0417300 Rorug06G0417400
rosa_samantha	Rh3BG325800 Rh3CG323300 Rh7BG015500 Rh7CG016200
rosa_wichuraiana	Rw0G013680 Rw7G001250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	28, 1324
AccII	CGCG	1 cut(s)	1068
AciI	CCGC	4 cut(s)	28, 63, 1137, 1324
AclWI	GGATC	4 cut(s)	812, 846, 1104, 1299
AcoI	YGGCCR	1 cut(s)	850
AcsI	RAATTY	3 cut(s)	418, 592, 821
AcuI	CTGAAG	3 cut(s)	362, 678, 863
AfaI	GTAC	1 cut(s)	1297
AfiI	CCNNNNNNNGG	3 cut(s)	161, 531, 532
AgsI	TTSAA	7 cut(s)	448, 562, 602, 1007, 1088, 1129, 1309
AjnI	CCWGG	3 cut(s)	847, 1132, 1365
AjuI	GAANNNNNNNTTGG	2 cut(s)	963, 995
AleI	CACNNNNGTG	1 cut(s)	576
AloI	GAACNNNNNNTCC	2 cut(s)	624, 656
AluBI	AGCT	8 cut(s)	188, 218, 235, 635, 977, 1241, 1313, 1420
AluI	AGCT	8 cut(s)	188, 218, 235, 635, 977, 1241, 1313, 1420
Alw21I	GWGCWC	2 cut(s)	60, 797
Alw26I	GTCTC	1 cut(s)	492
AlwI	GGATC	4 cut(s)	812, 846, 1104, 1299
AoxI	GGCC	6 cut(s)	81, 102, 225, 850, 903, 1453
ApeKI	GCWGC	1 cut(s)	5
ApoI	RAATTY	3 cut(s)	418, 592, 821
ArsI	GACNNNNNNTTYG	4 cut(s)	981, 1013, 1031, 1063
AseI	ATTAAT	1 cut(s)	285
AspLEI	GCGC	2 cut(s)	320, 995
AspS9I	GGNCC	1 cut(s)	1453
AsuC2I	CCSGG	1 cut(s)	589
AsuHPI	GGTGA	2 cut(s)	324, 902
AxyI	CCTNAGG	1 cut(s)	132
BaeI	ACNNNNGTAYC	2 cut(s)	396, 429
BalI	TGGCCA	1 cut(s)	852
Bbv12I	GWGCWC	2 cut(s)	60, 797
BbvCI	CCTCAGC	1 cut(s)	1287
BccI	CCATC	4 cut(s)	430, 435, 1189, 1401
BciT130I	CCWGG	3 cut(s)	849, 1134, 1367
BclI	TGATCA	2 cut(s)	1300, 1422
BcnI	CCSGG	1 cut(s)	589
BcoDI	GTCTC	1 cut(s)	492
BfmI	CTRYAG	1 cut(s)	1162
BglII	AGATCT	2 cut(s)	109, 666
BisI	GCNGC	2 cut(s)	6, 1138
BlsI	GCNGC	2 cut(s)	7, 1139
Bme1390I	CCNGG	4 cut(s)	589, 849, 1134, 1367
BmgT120I	GGNCC	1 cut(s)	1453
BmiI	GGNNCC	3 cut(s)	537, 713, 1454
BmrFI	CCNGG	4 cut(s)	589, 849, 1134, 1367
BmrI	ACTGGG	1 cut(s)	58
BmsI	GCATC	4 cut(s)	78, 888, 1242, 1252
BmuI	ACTGGG	1 cut(s)	58
BpmI	CTGGAG	2 cut(s)	228, 516
Bpu10I	CCTNAGC	1 cut(s)	1287
BpuEI	CTTGAG	2 cut(s)	1241, 1362
BpuMI	CCSGG	1 cut(s)	589
BsaJI	CCNNGG	2 cut(s)	99, 552
BsaXI	ACNNNNNCTCC	2 cut(s)	624, 654
Bsc4I	CCNNNNNNNGG	3 cut(s)	161, 531, 532
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	4 cut(s)	53, 211, 754, 1335
Bse21I	CCTNAGG	1 cut(s)	132
Bse3DI	GCAATG	2 cut(s)	1281, 1461
BseBI	CCWGG	3 cut(s)	849, 1134, 1367
BseDI	CCNNGG	2 cut(s)	99, 552
BseGI	GGATG	4 cut(s)	438, 446, 1267, 1412
BseLI	CCNNNNNNNGG	3 cut(s)	161, 531, 532
BseMI	GCAATG	2 cut(s)	1281, 1461
BseMII	CTCAG	3 cut(s)	120, 123, 1278
BseNI	ACTGG	4 cut(s)	53, 211, 754, 1335
BseYI	CCCAGC	1 cut(s)	188
BsgI	GTGCAG	1 cut(s)	56
Bsh1236I	CGCG	1 cut(s)	1068
BshFI	GGCC	6 cut(s)	83, 104, 227, 852, 905, 1455
BsiHKAI	GWGCWC	2 cut(s)	60, 797
BsiSI	CCGG	3 cut(s)	228, 588, 1437
BslFI	GGGAC	1 cut(s)	674
BslI	CCNNNNNNNGG	3 cut(s)	161, 531, 532
BsmAI	GTCTC	1 cut(s)	492
BsmFI	GGGAC	1 cut(s)	674
BsnI	GGCC	6 cut(s)	83, 104, 227, 852, 905, 1455
Bsp1286I	GDGCHC	2 cut(s)	60, 797
Bsp68I	TCGCGA	1 cut(s)	1068
BspACI	CCGC	4 cut(s)	28, 63, 1137, 1324
BspANI	GGCC	6 cut(s)	83, 104, 227, 852, 905, 1455
BspCNI	CTCAG	3 cut(s)	119, 124, 1279
BspFNI	CGCG	1 cut(s)	1068
BspLI	GGNNCC	3 cut(s)	537, 713, 1454
BspPI	GGATC	4 cut(s)	812, 846, 1104, 1299
BspQI	GCTCTTC	1 cut(s)	18
BsrBI	CCGCTC	2 cut(s)	28, 1324
BsrDI	GCAATG	2 cut(s)	1281, 1461
BsrFI	RCCGGY	1 cut(s)	227
BsrI	ACTGG	4 cut(s)	53, 211, 754, 1335
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	2 cut(s)	99, 552
BssT1I	CCWWGG	2 cut(s)	99, 552
Bst2UI	CCWGG	3 cut(s)	849, 1134, 1367
Bst4CI	ACNGT	4 cut(s)	76, 260, 1064, 1163
Bst6I	CTCTTC	1 cut(s)	18
BstC8I	GCNNGC	3 cut(s)	1098, 1267, 1442
BstDEI	CTNAG	4 cut(s)	106, 132, 724, 1287
BstF5I	GGATG	4 cut(s)	438, 446, 1267, 1412
BstFNI	CGCG	1 cut(s)	1068
BstHHI	GCGC	2 cut(s)	320, 995
BstMAI	GTCTC	1 cut(s)	492
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 34, 145, 224
BstNI	CCWGG	3 cut(s)	849, 1134, 1367
BstNSI	RCATGY	1 cut(s)	577
BstSCI	CCNGG	4 cut(s)	587, 847, 1132, 1365
BstSFI	CTRYAG	1 cut(s)	1162
BstUI	CGCG	1 cut(s)	1068
BstX2I	RGATCY	3 cut(s)	109, 666, 838
BstXI	CCANNNNNNTGG	1 cut(s)	494
BstYI	RGATCY	3 cut(s)	109, 666, 838
Bsu36I	CCTNAGG	1 cut(s)	132
BsuRI	GGCC	6 cut(s)	83, 104, 227, 852, 905, 1455
BtsCI	GGATG	4 cut(s)	438, 446, 1267, 1412
BtsIMutI	CAGTG	3 cut(s)	204, 265, 1159
BtuMI	TCGCGA	1 cut(s)	1068
Cac8I	GCNNGC	3 cut(s)	1098, 1267, 1442
CfoI	GCGC	2 cut(s)	320, 995
Cfr10I	RCCGGY	1 cut(s)	227
Cfr13I	GGNCC	1 cut(s)	1453
Csp6I	GTAC	1 cut(s)	1296
CviAII	CATG	4 cut(s)	85, 313, 574, 1229
CviQI	GTAC	1 cut(s)	1296
DdeI	CTNAG	4 cut(s)	106, 132, 724, 1287
EaeI	YGGCCR	1 cut(s)	850
Eam1104I	CTCTTC	1 cut(s)	18
EarI	CTCTTC	1 cut(s)	18
EciI	GGCGGA	1 cut(s)	52
Eco130I	CCWWGG	2 cut(s)	99, 552
Eco147I	AGGCCT	1 cut(s)	104
Eco57I	CTGAAG	3 cut(s)	362, 678, 863
Eco81I	CCTNAGG	1 cut(s)	132
EcoO109I	RGGNCCY	1 cut(s)	1453
EcoRII	CCWGG	3 cut(s)	847, 1132, 1365
EcoT14I	CCWWGG	2 cut(s)	99, 552
ErhI	CCWWGG	2 cut(s)	99, 552
FaeI	CATG	4 cut(s)	88, 316, 577, 1232
FaqI	GGGAC	1 cut(s)	674
FatI	CATG	4 cut(s)	84, 312, 573, 1228
FauI	CCCGC	2 cut(s)	21, 1317
FbaI	TGATCA	2 cut(s)	1300, 1422
Fnu4HI	GCNGC	2 cut(s)	6, 1138
FokI	GGATG	4 cut(s)	445, 453, 1274, 1419
Fsp4HI	GCNGC	2 cut(s)	6, 1138
GlaI	GCGC	2 cut(s)	319, 994
GluI	GCNGC	2 cut(s)	6, 1138
GsaI	CCCAGC	1 cut(s)	192
GsuI	CTGGAG	2 cut(s)	228, 516
HaeIII	GGCC	6 cut(s)	83, 104, 227, 852, 905, 1455
HapII	CCGG	3 cut(s)	228, 588, 1437
HhaI	GCGC	2 cut(s)	320, 995
Hin1II	CATG	4 cut(s)	88, 316, 577, 1232
Hin6I	GCGC	2 cut(s)	318, 993
HinP1I	GCGC	2 cut(s)	318, 993
HindIII	AAGCTT	1 cut(s)	1239
HinfI	GANTC	8 cut(s)	95, 476, 500, 584, 655, 984, 1355, 1433
HpaII	CCGG	3 cut(s)	228, 588, 1437
HphI	GGTGA	2 cut(s)	324, 902
Hpy166II	GTNNAC	1 cut(s)	458
Hpy188III	TCNNGA	9 cut(s)	113, 495, 790, 863, 968, 1037, 1067, 1088, 1379
Hpy8I	GTNNAC	1 cut(s)	458
HpyAV	CCTTC	5 cut(s)	442, 702, 1123, 1339, 1405
HpyCH4III	ACNGT	4 cut(s)	76, 260, 1064, 1163
HpyCH4IV	ACGT	1 cut(s)	303
HpyCH4V	TGCA	4 cut(s)	37, 621, 1020, 1100
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 34, 145, 224
HpyF3I	CTNAG	4 cut(s)	106, 132, 724, 1287
HpySE526I	ACGT	1 cut(s)	303
Hsp92II	CATG	4 cut(s)	88, 316, 577, 1232
HspAI	GCGC	2 cut(s)	318, 993
Ksp22I	TGATCA	2 cut(s)	1300, 1422
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	6 cut(s)	55, 153, 185, 535, 632, 1321
LweI	GCATC	4 cut(s)	78, 888, 1242, 1252
MaeII	ACGT	1 cut(s)	303
MaeIII	GTNAC	2 cut(s)	325, 890
MbiI	CCGCTC	2 cut(s)	28, 1324
MboII	GAAGA	5 cut(s)	35, 52, 134, 283, 355
MflI	RGATCY	3 cut(s)	109, 666, 838
MhlI	GDGCHC	2 cut(s)	60, 797
MlsI	TGGCCA	1 cut(s)	852
MluCI	AATT	7 cut(s)	212, 379, 418, 592, 821, 933, 1474
MluNI	TGGCCA	1 cut(s)	852
MlyI	GAGTC	3 cut(s)	89, 494, 978
MmeI	TCCRAC	2 cut(s)	540, 816
Mox20I	TGGCCA	1 cut(s)	852
MscI	TGGCCA	1 cut(s)	852
MseI	TTAA	2 cut(s)	285, 1206
MslI	CAYNNNNRTG	1 cut(s)	576
Msp20I	TGGCCA	1 cut(s)	852
MspI	CCGG	3 cut(s)	228, 588, 1437
MspR9I	CCNGG	4 cut(s)	589, 849, 1134, 1367
MvaI	CCWGG	3 cut(s)	849, 1134, 1367
MvnI	CGCG	1 cut(s)	1068
MwoI	GCNNNNNNNGC	4 cut(s)	11, 34, 145, 224
NciI	CCSGG	1 cut(s)	589
NlaIII	CATG	4 cut(s)	88, 316, 577, 1232
NlaIV	GGNNCC	3 cut(s)	537, 713, 1454
NmuCI	GTSAC	2 cut(s)	325, 890
NruI	TCGCGA	1 cut(s)	1068
NspI	RCATGY	1 cut(s)	577
OliI	CACNNNNGTG	1 cut(s)	576
PceI	AGGCCT	1 cut(s)	104
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	1 cut(s)	766
PfeI	GAWTC	5 cut(s)	476, 584, 655, 1355, 1433
PfoI	TCCNGGA	2 cut(s)	587, 1365
PkrI	GCNGC	2 cut(s)	7, 1139
PleI	GAGTC	3 cut(s)	89, 494, 978
PpsI	GAGTC	3 cut(s)	89, 494, 978
PshBI	ATTAAT	1 cut(s)	285
Psp6I	CCWGG	3 cut(s)	847, 1132, 1365
PspFI	CCCAGC	1 cut(s)	188
PspGI	CCWGG	3 cut(s)	847, 1132, 1365
PspN4I	GGNNCC	3 cut(s)	537, 713, 1454
PspPI	GGNCC	1 cut(s)	1453
PsuI	RGATCY	3 cut(s)	109, 666, 838
RruI	TCGCGA	1 cut(s)	1068
RsaI	GTAC	1 cut(s)	1297
RsaNI	GTAC	1 cut(s)	1296
RseI	CAYNNNNRTG	1 cut(s)	576
SapI	GCTCTTC	1 cut(s)	18
SaqAI	TTAA	2 cut(s)	285, 1206
SatI	GCNGC	2 cut(s)	6, 1138
Sau96I	GGNCC	1 cut(s)	1453
SchI	GAGTC	3 cut(s)	89, 494, 978
ScrFI	CCNGG	4 cut(s)	589, 849, 1134, 1367
SduI	GDGCHC	2 cut(s)	60, 797
SfaNI	GCATC	4 cut(s)	78, 888, 1242, 1252
SfcI	CTRYAG	1 cut(s)	1162
SmiMI	CAYNNNNRTG	1 cut(s)	576
SmlI	CTYRAG	2 cut(s)	1256, 1377
SmoI	CTYRAG	2 cut(s)	1256, 1377
Sse9I	AATT	7 cut(s)	212, 379, 418, 592, 821, 933, 1474
SseBI	AGGCCT	1 cut(s)	104
SsiI	CCGC	4 cut(s)	28, 63, 1137, 1324
StuI	AGGCCT	1 cut(s)	104
StyD4I	CCNGG	4 cut(s)	587, 847, 1132, 1365
StyI	CCWWGG	2 cut(s)	99, 552
TaaI	ACNGT	4 cut(s)	76, 260, 1064, 1163
TaiI	ACGT	1 cut(s)	306
TaqI	TCGA	6 cut(s)	93, 114, 807, 864, 969, 999
TasI	AATT	7 cut(s)	212, 379, 418, 592, 821, 933, 1474
TauI	GCSGC	1 cut(s)	1140
TfiI	GAWTC	5 cut(s)	476, 584, 655, 1355, 1433
Tru1I	TTAA	2 cut(s)	285, 1206
Tru9I	TTAA	2 cut(s)	285, 1206
TscAI	CASTG	3 cut(s)	211, 265, 1159
TseFI	GTSAC	2 cut(s)	325, 890
TseI	GCWGC	1 cut(s)	5
Tsp45I	GTSAC	2 cut(s)	325, 890
TspDTI	ATGAA	5 cut(s)	241, 291, 301, 1347, 1450
TspGWI	ACGGA	3 cut(s)	422, 934, 1327
TspRI	CASTG	3 cut(s)	211, 265, 1159
VspI	ATTAAT	1 cut(s)	285
XapI	RAATTY	3 cut(s)	418, 592, 821
XceI	RCATGY	1 cut(s)	577

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.