Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006892

Physical Location & Seq

Genomic Coordinates Forward (+)

36249835 .. 36255290

Gene Structure 5456 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1231400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3141 bp

ATGGTGTTCAAGGCCAGGTTCTTCTCCTCCAAGAAGTCCGACTCCTCCAACAGCCCCGATGGCTCCAATAATAGTCCGCGATCTCTGGGTTCCAACTCCCCGATCGGATCCGATAAGAAGAAGCCCAAATCCGCGTCCAAAGACGACTCGCCGGGCCCGAGTACCAGTTCCGGCGGCGGAGGGGGCGGCTTCAGTCGGCGGAGTCTAGTCAAAGACTTGTCAAAGAAGAAGGATGTGAAGGGGAAAGAGAGTCCAGCTCAGCTGAAGACGCCGGCGAAGTCCGCGCGCTCGACTGGCGCGACTCCCAAGAAGTCGTCTGCGTCCACATCGGGGGCGGCGGAGTCTGCGGCGGAGGTTTCTCCCATTCTGGCGTCGTCGCTGGGGTTGAATAGGATAAAGACGAGATCGGGGCCGTTACCGCAGGAGAGCTTTTTCGGGTATAGGGGCGACAAAGGGTCGGCGCTGGCCTCCAGTAATTTGTCTAGGCCGGGCGTCGGCGGCGATCGTTTAGGGTCGGGCATTGGGGGGAAGAAGAAGGAGGCTGCGAGTCAGACTAGGATTGGGTTCAATGAAAATGTGGCAAGTGGGAATTGGGGTGATAATGGCAGCAATTCTGATGCTGCATCAACTGGTAGCTTGCCTTCGAGAGACCAGAGCCCGAACGTGGTGGCGCCATCGCGGTTACACAACGGGGAATCGTCGGCAGAAGCTGGGAGAAACATATCATCTTGGGGTCACTCTGGAGGCTTAAGAAGTTCAGATGTATGTACACCAGAGACTGCATATGATTGTGAAAATCCTAAGGAGTCCGAGTCTCCTCGTTTTCAAGCTATACTCCGCTTAACGAGTGCACCAAGAAAGAGGTTTCCTGCTGATATAAAAAGTTTTTCCCATGAACTCAATTCAAAAGGCGTAAGACCCTTCCCATTTTGGAAGCCTCGAGGCTTGAACAACCTGGAGGAAATCTTGGTTGCGATTAGGGCAAAGTTTGACAAAGCAAAGGAAGAAGTGAACTCTGACCTGGCTATATTTGCAGCAGATTTAGTTGGAATTCTTGAAAAAGATGCAGATAATCATCCAGAGTGGCAGGAAACTCTTGAGGATTTGCTGGTTTTGGCTCGAAGTTGTGCAATGACATCATCTGGAGAGTTTTGGCTTCAGTGTGAGGGCATAGTTCAGGAATTGGATGATAGACGTCAAGAACTTCCTCCGGGGGTGCTGAAGCAGCTTCACACTCGAATGCTTTTCATACTCACAAGGTGTACTAGGTTGCTGCAATTCCATAAGGAAACTGGATTGGCTGAGGATGAACAGGTTTTCCAGCTTCGCCAATCAAGAGTTCTGCGTTTCTCTGATAAAAGAATTCCTCCAAGTTTGGCAAAGGATACAAAGAATTTAAGTGTTGCAAAAGCCTCTAAAGCAGGTTCAGCTAGGAAATTTTACAGTCAGGAGCAACATGGCTTGGAATGGAAGAGAGACCATGTTATACAACCGGGAAATTTGTTCGCACCTCCTGTTGATCAACCCTCTAAGAATTTGGAGTCTCCTGCTGGTAGGGACCGGATGACATCATGGAAGAAATTCCCATCTCTGGTTGCAAAAAACATGAAAGAAGCTACTGAATTTAAGGAGCAGAGTGATTCTAAGGTTGAACATTTGAAAGGTTCAGACAACAAGAGAGGGACTTCTAACATCGATCCTACTTCTGTTAAGCCTCCTGAGCCTTCTGGCAAGGGTTCTCATGAACGTTCTTCCAAGCACCAGCACAAACCTTCTTGGGGTTGGGGAAATCAACAAAATGTATCTGATGATAGTTCTATAATATGTCGCATTTGTGAGGAGGAGGTTCCAACTGCAAATGTGGAAGACCATTCAAGAATTTGTGCAATTGCTGATCGATGTGATAAGAAAGGTATAAGTGTGAATGAACGTCTTGTCATAATTTCTGAAACCCTTGAAAAACTGATGGAGTCCTTTGCTCAAAAGGATATCCAGCATGGTGTAGGAAGTCCAGACATTGCGAAAGCATCTAGTTCAAGCGTGACAGAAGAATCAGAGGTTCTATCTCCAAAACTTAGTGACTGGTCCCACAGAGGCTCAGAGGACATGCTTGATTGTTTTCCTGAAGCTGATAATTCATCTTTTATGGATGACCTGAAAGGTCTACCTTCCATGTCATGTAGAACTCGTTTTGCTCCAAAGTCAGATCAAGGAATGACAACATCATCTGCAGGCAGCATGACTCCTAGGTCTCCCCTATTGACCCCAAGTGGCAGTCAGATTGACTTGCTCCTCGCAGGCAAGGCTTCTTTTTCTGAGCAAGACGATATACCTCAGATGAATGAACTTTCTGATATTGCCCGGTGCATAGCAAATACACCTTTGGATGATGATCGCTCCATGCCATATCTACTTACTTGTCTTGAAGACTTGAGAGTTGTTATAGACCGCAGAAAGTTTGATGCACTTACTGTGGACACATTTGGGGCACGCATTGAGAAGCTGATTCGGTGGAAGTATTTGCTGCTTTGTGAGTTAGTGGATGATGAGAAGGTTGATTTAGCAACTACCGTAATTGATGAAGAAGCTCCTTTGGAAGATGACGTGGTTCGTACAAGCCCTATACCTTTTTCCAAGGACCGAACATCTATAGATGATTTTGAAATTATCAAACCAATAAGTCGTGGGGCATTTGGACGAGTTTTCTTAGCTAAAAAGAGAACAACAGGGGATCTTTTTGCGATTAAGGTTCTTAAAAAGGCTGATATGATCCGAAAGAATGCTGTTGAAAGTATATTAGCAGAACGTGACATCTTGATTTCAGTCCGCAATCCTTTTGTGGTTCGCTTCTTCTATTCTTTTACTTGTCGTGAAAACTTGTATCTTGTGATGGAGTATTTGAATGGAGGAGATCTATACTCTTTGTTGAGGAATTTAGGCTGCTTGGACGAGGAGGTTGCTCGTATATATATAGCAGAAGTGGTGCTTGCTTTAGAATATTTACATTCACTTCGTGTGGTTCATCGTGATTTGAAGCCTGACAATTTGTTAATTGCTCATGACGGCCATGTTAAGTGTCAATATAATAGTGGTTATATTATCGTTAGAGTTTTTCAGTTGAAGCAGGATAGTGAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1047

Amino Acids

115.08

Weight (kDa)

7.77

Isoelectric Point (pI)

48.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	311 - 429	2.5e-56	IREH1-like domain
Pkinase	PF00069	887 - 1025	2.4e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	888 - 1025	8e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17850
fragaria_vesca	FvH4_5g17560 FvH4_5g17560 FvH4_5g17560 FvH4_5g17560
malus_domestica	MD06G1231400.v1.1 MD06G1231500.v1.1 MD14G1238200.v1.1
prunus_persica	Prupe.5G235400_v2.0.a1
pyrus_communis	pycom06g20530 pycom06g20540 pycom14g19930 pycom14g19940
rosa_chinensis	RchiOBHm_Chr7g0178091
rosa_laevigata	RLG00000005472
rosa_multiflora	Rmu_co8223356.1_g000001 Rmu_sc0000425.1_g000008
rosa_roxburghii	Rroxscaffold_3G00275190
rosa_rugosa	Rorug06G0413400 Rorug06G0413500 Rorug06G0413500
rosa_samantha	Rh7AG012700 Rh7BG012600 Rh7CG012600 Rh7DG012900
rosa_wichuraiana	Rw7G001000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2248
AatII	GACGTC	1 cut(s)	1198
AbsI	CCTCGAGG	1 cut(s)	939
Acc36I	ACCTGC	1 cut(s)	1412
AccB1I	GGYRCC	1 cut(s)	670
AccI	GTMKAC	1 cut(s)	2164
AccII	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
AclI	AACGTT	1 cut(s)	1747
AclWI	GGATC	5 cut(s)	102, 115, 1691, 2739, 2764
AcoI	YGGCCR	1 cut(s)	3064
AcuI	CTGAAG	5 cut(s)	175, 284, 1142, 1241, 2145
AcyI	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
AdeI	CACNNNGTG	2 cut(s)	1260, 3014
AfaI	GTAC	4 cut(s)	163, 769, 1264, 2614
AfiI	CCNNNNNNNGG	5 cut(s)	330, 494, 664, 1591, 1778
AflII	CTTAAG	1 cut(s)	748
AhdI	GACNNNNNGTC	1 cut(s)	454
AjiI	CACGTC	1 cut(s)	2605
AjnI	CCWGG	3 cut(s)	14, 954, 1020
AjuI	GAANNNNNNNTTGG	2 cut(s)	1323, 1355
AloI	GAACNNNNNNTCC	2 cut(s)	1302, 1334
Alw21I	GWGCWC	1 cut(s)	853
Alw26I	GTCTC	6 cut(s)	642, 770, 819, 1470, 1548, 2256
Alw44I	GTGCAC	1 cut(s)	849
AlwI	GGATC	5 cut(s)	102, 115, 1691, 2739, 2764
AlwNI	CAGNNNCTG	2 cut(s)	710, 779
Ama87I	CYCGRG	2 cut(s)	157, 939
AoxI	GGCC	6 cut(s)	12, 154, 410, 465, 485, 3064
ApaI	GGGCCC	1 cut(s)	158
ApaLI	GTGCAC	1 cut(s)	849
ApeKI	GCWGC	9 cut(s)	542, 606, 620, 1034, 1225, 1273, 2235, 2524, 2940
Asp700I	GAANNNNTTC	1 cut(s)	1580
AspA2I	CCTAGG	1 cut(s)	2246
AspLEI	GCGC	5 cut(s)	286, 288, 299, 463, 673
AspS9I	GGNCC	6 cut(s)	154, 155, 410, 1558, 2085, 2638
AsuC2I	CCSGG	5 cut(s)	153, 489, 1212, 1494, 2362
AsuHPI	GGTGA	1 cut(s)	608
AvaI	CYCGRG	2 cut(s)	157, 939
AvaII	GGWCC	3 cut(s)	1558, 2085, 2638
AvrII	CCTAGG	1 cut(s)	2246
AxyI	CCTNAGG	1 cut(s)	801
BaeGI	GKGCMC	3 cut(s)	158, 853, 2491
BamHI	GGATCC	1 cut(s)	107
BanI	GGYRCC	1 cut(s)	670
BanII	GRGCYC	2 cut(s)	158, 659
BarI	GAAGNNNNNNTAC	2 cut(s)	625, 657
BbsI	GAAGAC	3 cut(s)	272, 1872, 2433
Bbv12I	GWGCWC	1 cut(s)	853
BbvCI	CCTCAGC	1 cut(s)	1302
BbvI	GCAGC	9 cut(s)	529, 607, 618, 1046, 1237, 1260, 2247, 2511, 2927
BccI	CCATC	5 cut(s)	53, 682, 1594, 1960, 2884
BceAI	ACGGC	2 cut(s)	397, 3079
BcgI	CGANNNNNNTGC	2 cut(s)	2939, 2973
BciT130I	CCWGG	3 cut(s)	16, 956, 1022
BciVI	GTATCC	1 cut(s)	1378
BclI	TGATCA	1 cut(s)	1519
BcnI	CCSGG	5 cut(s)	153, 489, 1212, 1494, 2362
BcoDI	GTCTC	6 cut(s)	642, 770, 819, 1470, 1548, 2256
BfaI	CTAG	7 cut(s)	206, 483, 555, 1266, 1431, 2031, 2247
BfmI	CTRYAG	2 cut(s)	2229, 2649
BfoI	RGCGCY	2 cut(s)	464, 674
BfrI	CTTAAG	1 cut(s)	748
BfuAI	ACCTGC	1 cut(s)	1412
BfuI	GTATCC	1 cut(s)	1378
BglI	GCCNNNNNGGC	1 cut(s)	60
BglII	AGATCT	1 cut(s)	2911
BlnI	CCTAGG	1 cut(s)	2246
BlpI	GCTNAGC	1 cut(s)	258
Bme1390I	CCNGG	8 cut(s)	16, 153, 489, 956, 1022, 1212, 1494, 2362
Bme18I	GGWCC	3 cut(s)	1558, 2085, 2638
BmeRI	GACNNNNNGTC	1 cut(s)	454
BmeT110I	CYCGRG	2 cut(s)	157, 939
BmgBI	CACGTC	1 cut(s)	2605
BmgT120I	GGNCC	6 cut(s)	154, 155, 410, 1558, 2085, 2638
BmiI	GGNNCC	9 cut(s)	64, 91, 109, 156, 411, 672, 1559, 1848, 2087
BmrFI	CCNGG	8 cut(s)	16, 153, 489, 956, 1022, 1212, 1494, 2362
BmsI	GCATC	5 cut(s)	607, 632, 1054, 2036, 2452
BpiI	GAAGAC	3 cut(s)	272, 1872, 2433
BplI	GAGNNNNNCTC	2 cut(s)	241, 273
BpmI	CTGGAG	4 cut(s)	454, 762, 977, 1164
Bpu10I	CCTNAGC	2 cut(s)	1302, 1719
Bpu1102I	GCTNAGC	1 cut(s)	258
BpuEI	CTTGAG	2 cut(s)	1118, 2452
BpuMI	CCSGG	5 cut(s)	153, 489, 1212, 1494, 2362
Bsa29I	ATCGAT	2 cut(s)	1695, 1897
BsaBI	GATNNNNATC	2 cut(s)	1074, 2391
BsaHI	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
BsaI	GGTCTC	3 cut(s)	642, 1470, 2256
BsaJI	CCNNGG	3 cut(s)	1211, 2246, 2634
BsaWI	WCCGGW	1 cut(s)	1560
BsaXI	ACNNNNNCTCC	2 cut(s)	797, 827
Bsc4I	CCNNNNNNNGG	5 cut(s)	330, 494, 664, 1591, 1778
Bse118I	RCCGGY	1 cut(s)	271
Bse1I	ACTGG	6 cut(s)	165, 298, 471, 634, 1297, 2087
Bse21I	CCTNAGG	1 cut(s)	801
Bse3DI	GCAATG	2 cut(s)	1137, 2016
Bse8I	GATNNNNATC	2 cut(s)	1074, 2391
BseBI	CCWGG	3 cut(s)	16, 956, 1022
BseCI	ATCGAT	2 cut(s)	1695, 1897
BseDI	CCNNGG	3 cut(s)	1211, 2246, 2634
BseGI	GGATG	8 cut(s)	238, 1075, 1192, 1312, 1569, 2155, 2392, 2548
BseJI	GATNNNNATC	2 cut(s)	1074, 2391
BseLI	CCNNNNNNNGG	5 cut(s)	330, 494, 664, 1591, 1778
BseMI	GCAATG	2 cut(s)	1137, 2016
BseMII	CTCAG	6 cut(s)	272, 1293, 1710, 2112, 2307, 2348
BseNI	ACTGG	6 cut(s)	165, 298, 471, 634, 1297, 2087
BsePI	GCGCGC	1 cut(s)	284
BseRI	GAGGAG	8 cut(s)	16, 34, 807, 1853, 1856, 2282, 2922, 2966
BseSI	GKGCMC	3 cut(s)	158, 853, 2491
BseXI	GCAGC	9 cut(s)	529, 607, 618, 1046, 1237, 1260, 2247, 2511, 2927
BseYI	CCCAGC	2 cut(s)	379, 710
Bsh1236I	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
Bsh1285I	CGRYCG	2 cut(s)	105, 505
BshFI	GGCC	6 cut(s)	14, 156, 412, 467, 487, 3066
BshNI	GGYRCC	1 cut(s)	670
BshVI	ATCGAT	2 cut(s)	1695, 1897
BsiEI	CGRYCG	2 cut(s)	105, 505
BsiHKAI	GWGCWC	1 cut(s)	853
BsiHKCI	CYCGRG	2 cut(s)	157, 939
BsiSI	CCGG	8 cut(s)	152, 171, 272, 488, 1211, 1493, 1561, 2362
BslFI	GGGAC	3 cut(s)	1571, 1696, 2071
BslI	CCNNNNNNNGG	5 cut(s)	330, 494, 664, 1591, 1778
BsmAI	GTCTC	6 cut(s)	642, 770, 819, 1470, 1548, 2256
BsmFI	GGGAC	3 cut(s)	1571, 1696, 2071
BsmI	GAATGC	2 cut(s)	1245, 2785
BsnI	GGCC	6 cut(s)	14, 156, 412, 467, 487, 3066
Bso31I	GGTCTC	3 cut(s)	642, 1470, 2256
BsoBI	CYCGRG	2 cut(s)	157, 939
Bsp120I	GGGCCC	1 cut(s)	154
Bsp1286I	GDGCHC	4 cut(s)	158, 659, 853, 2491
Bsp1407I	TGTACA	1 cut(s)	767
Bsp1720I	GCTNAGC	1 cut(s)	258
BspANI	GGCC	6 cut(s)	14, 156, 412, 467, 487, 3066
BspCNI	CTCAG	6 cut(s)	271, 1294, 1711, 2111, 2308, 2347
BspDI	ATCGAT	2 cut(s)	1695, 1897
BspFNI	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
BspHI	TCATGA	2 cut(s)	1741, 3058
BspLI	GGNNCC	9 cut(s)	64, 91, 109, 156, 411, 672, 1559, 1848, 2087
BspMAI	CTGCAG	1 cut(s)	2233
BspMI	ACCTGC	1 cut(s)	1412
BspPI	GGATC	5 cut(s)	102, 115, 1691, 2739, 2764
BspT107I	GGYRCC	1 cut(s)	670
BspTI	CTTAAG	1 cut(s)	748
BspTNI	GGTCTC	3 cut(s)	642, 1470, 2256
BsrDI	GCAATG	2 cut(s)	1137, 2016
BsrFI	RCCGGY	1 cut(s)	271
BsrGI	TGTACA	1 cut(s)	767
BsrI	ACTGG	6 cut(s)	165, 298, 471, 634, 1297, 2087
BssAI	RCCGGY	1 cut(s)	271
BssECI	CCNNGG	3 cut(s)	1211, 2246, 2634
BssHII	GCGCGC	1 cut(s)	284
BssNI	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
BssT1I	CCWWGG	2 cut(s)	2246, 2634
Bst2UI	CCWGG	3 cut(s)	16, 956, 1022
Bst4CI	ACNGT	3 cut(s)	1445, 2473, 2572
Bst6I	CTCTTC	1 cut(s)	1466
BstACI	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
BstAFI	CTTAAG	1 cut(s)	748
BstAUI	TGTACA	1 cut(s)	767
BstC8I	GCNNGC	8 cut(s)	273, 286, 465, 638, 2233, 2299, 2491, 2988
BstF5I	GGATG	8 cut(s)	238, 1075, 1192, 1312, 1569, 2155, 2392, 2548
BstFNI	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
BstH2I	RGCGCY	2 cut(s)	464, 674
BstHHI	GCGC	5 cut(s)	286, 288, 299, 463, 673
BstMAI	GTCTC	6 cut(s)	642, 770, 819, 1470, 1548, 2256
BstMCI	CGRYCG	2 cut(s)	105, 505
BstNI	CCWGG	3 cut(s)	16, 956, 1022
BstNSI	RCATGY	1 cut(s)	2110
BstSCI	CCNGG	8 cut(s)	14, 151, 487, 954, 1020, 1210, 1492, 2360
BstSFI	CTRYAG	2 cut(s)	2229, 2649
BstSLI	GKGCMC	3 cut(s)	158, 853, 2491
BstUI	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
BstV1I	GCAGC	9 cut(s)	529, 607, 618, 1046, 1237, 1260, 2247, 2511, 2927
BstV2I	GAAGAC	3 cut(s)	272, 1872, 2433
BstX2I	RGATCY	3 cut(s)	107, 2731, 2911
BstYI	RGATCY	3 cut(s)	107, 2731, 2911
Bsu15I	ATCGAT	2 cut(s)	1695, 1897
Bsu36I	CCTNAGG	1 cut(s)	801
BsuI	GTATCC	1 cut(s)	1378
BsuRI	GGCC	6 cut(s)	14, 156, 412, 467, 487, 3066
BsuTUI	ATCGAT	2 cut(s)	1695, 1897
BtgZI	GCGATG	1 cut(s)	660
BtrI	CACGTC	1 cut(s)	2605
BtsCI	GGATG	8 cut(s)	238, 1075, 1192, 1312, 1569, 2155, 2392, 2548
BtsIMutI	CAGTG	1 cut(s)	1166
BveI	ACCTGC	1 cut(s)	1412
Cac8I	GCNNGC	8 cut(s)	273, 286, 465, 638, 2233, 2299, 2491, 2988
CaiI	CAGNNNCTG	2 cut(s)	710, 779
CciI	TCATGA	2 cut(s)	1741, 3058
CfoI	GCGC	5 cut(s)	286, 288, 299, 463, 673
Cfr10I	RCCGGY	1 cut(s)	271
Cfr13I	GGNCC	6 cut(s)	154, 155, 410, 1558, 2085, 2638
ClaI	ATCGAT	2 cut(s)	1695, 1897
CseI	GACGC	5 cut(s)	123, 277, 309, 360, 481
Csp6I	GTAC	4 cut(s)	162, 768, 1263, 2613
CviQI	GTAC	4 cut(s)	162, 768, 1263, 2613
DinI	GGCGCC	1 cut(s)	672
DraIII	CACNNNGTG	2 cut(s)	1260, 3014
DrdI	GACNNNNNNGTC	1 cut(s)	2248
DriI	GACNNNNNGTC	1 cut(s)	454
DseDI	GACNNNNNNGTC	1 cut(s)	2248
EaeI	YGGCCR	1 cut(s)	3064
Eam1104I	CTCTTC	1 cut(s)	1466
Eam1105I	GACNNNNNGTC	1 cut(s)	454
EarI	CTCTTC	1 cut(s)	1466
EciI	GGCGGA	4 cut(s)	192, 214, 353, 365
Eco130I	CCWWGG	2 cut(s)	2246, 2634
Eco24I	GRGCYC	2 cut(s)	158, 659
Eco31I	GGTCTC	3 cut(s)	642, 1470, 2256
Eco32I	GATATC	1 cut(s)	1990
Eco47I	GGWCC	3 cut(s)	1558, 2085, 2638
Eco57I	CTGAAG	5 cut(s)	175, 284, 1142, 1241, 2145
Eco81I	CCTNAGG	1 cut(s)	801
Eco88I	CYCGRG	2 cut(s)	157, 939
EcoRI	GAATTC	2 cut(s)	1050, 1362
EcoRII	CCWGG	3 cut(s)	14, 954, 1020
EcoRV	GATATC	1 cut(s)	1990
EcoT14I	CCWWGG	2 cut(s)	2246, 2634
EcoT38I	GRGCYC	2 cut(s)	158, 659
EgeI	GGCGCC	1 cut(s)	672
EheI	GGCGCC	1 cut(s)	672
ErhI	CCWWGG	2 cut(s)	2246, 2634
FalI	AAGNNNNNCTT	2 cut(s)	2970, 3002
FaqI	GGGAC	3 cut(s)	1571, 1696, 2071
FauNDI	CATATG	1 cut(s)	784
FbaI	TGATCA	1 cut(s)	1519
FblI	GTMKAC	1 cut(s)	2164
FokI	GGATG	8 cut(s)	245, 1062, 1199, 1319, 1576, 2162, 2399, 2555
FriOI	GRGCYC	2 cut(s)	158, 659
FspBI	CTAG	7 cut(s)	206, 483, 555, 1266, 1431, 2031, 2247
GlaI	GCGC	5 cut(s)	285, 287, 298, 462, 672
GsaI	CCCAGC	2 cut(s)	383, 714
GsuI	CTGGAG	4 cut(s)	454, 762, 977, 1164
HaeII	RGCGCY	2 cut(s)	464, 674
HaeIII	GGCC	6 cut(s)	14, 156, 412, 467, 487, 3066
HapII	CCGG	8 cut(s)	152, 171, 272, 488, 1211, 1493, 1561, 2362
HgaI	GACGC	5 cut(s)	123, 277, 309, 360, 481
HhaI	GCGC	5 cut(s)	286, 288, 299, 463, 673
Hin1I	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
Hin6I	GCGC	5 cut(s)	284, 286, 297, 461, 671
HinP1I	GCGC	5 cut(s)	284, 286, 297, 461, 671
HpaII	CCGG	8 cut(s)	152, 171, 272, 488, 1211, 1493, 1561, 2362
HphI	GGTGA	1 cut(s)	608
Hpy166II	GTNNAC	7 cut(s)	324, 770, 851, 1012, 1263, 2165, 2476
Hpy8I	GTNNAC	7 cut(s)	324, 770, 851, 1012, 1263, 2165, 2476
Hpy99I	CGWCG	4 cut(s)	376, 379, 497, 703
HpyAV	CCTTC	9 cut(s)	223, 232, 529, 651, 931, 1734, 1782, 2178, 2545
HpyCH4III	ACNGT	3 cut(s)	1445, 2473, 2572
HpyCH4IV	ACGT	6 cut(s)	663, 1195, 1747, 1930, 2604, 2806
HpySE526I	ACGT	6 cut(s)	663, 1195, 1747, 1930, 2604, 2806
Hsp92I	GRCGYC	5 cut(s)	269, 371, 492, 671, 1195
HspAI	GCGC	5 cut(s)	284, 286, 297, 461, 671
KasI	GGCGCC	1 cut(s)	670
KroI	GCCGGC	1 cut(s)	271
KroNI	GCCGGC	1 cut(s)	273
Ksp22I	TGATCA	1 cut(s)	1519
LmnI	GCTCC	7 cut(s)	68, 1450, 1630, 2200, 2295, 2402, 2593
Lsp1109I	GCAGC	9 cut(s)	529, 607, 618, 1046, 1237, 1260, 2247, 2511, 2927
LweI	GCATC	5 cut(s)	607, 632, 1054, 2036, 2452
MaeI	CTAG	7 cut(s)	206, 483, 555, 1266, 1431, 2031, 2247
MaeII	ACGT	6 cut(s)	663, 1195, 1747, 1930, 2604, 2806
MaeIII	GTNAC	6 cut(s)	414, 681, 734, 2041, 2078, 2807
MfeI	CAATTG	1 cut(s)	1887
MflI	RGATCY	3 cut(s)	107, 2731, 2911
MhlI	GDGCHC	4 cut(s)	158, 659, 853, 2491
Mly113I	GGCGCC	1 cut(s)	671
MmeI	TCCRAC	5 cut(s)	63, 72, 117, 1027, 1874
MreI	CGCCGGCG	1 cut(s)	271
MroNI	GCCGGC	1 cut(s)	271
MroXI	GAANNNNTTC	1 cut(s)	1580
MseI	TTAA	9 cut(s)	749, 842, 1397, 1626, 1710, 2745, 2754, 3050, 3072
MslI	CAYNNNNRTG	1 cut(s)	1238
MspA1I	CMGCKG	1 cut(s)	262
MspCI	CTTAAG	1 cut(s)	748
MspI	CCGG	8 cut(s)	152, 171, 272, 488, 1211, 1493, 1561, 2362
MspR9I	CCNGG	8 cut(s)	16, 153, 489, 956, 1022, 1212, 1494, 2362
MunI	CAATTG	1 cut(s)	1887
Mva1269I	GAATGC	2 cut(s)	1245, 2785
MvaI	CCWGG	3 cut(s)	16, 956, 1022
MvnI	CGCG	6 cut(s)	79, 134, 284, 286, 299, 679
NaeI	GCCGGC	1 cut(s)	273
NarI	GGCGCC	1 cut(s)	671
NciI	CCSGG	5 cut(s)	153, 489, 1212, 1494, 2362
NdeI	CATATG	1 cut(s)	784
NgoMIV	GCCGGC	1 cut(s)	271
NlaIV	GGNNCC	9 cut(s)	64, 91, 109, 156, 411, 672, 1559, 1848, 2087
NmuCI	GTSAC	4 cut(s)	734, 2041, 2078, 2807
NspI	RCATGY	1 cut(s)	2110
PaeR7I	CTCGAG	1 cut(s)	939
PagI	TCATGA	2 cut(s)	1741, 3058
PauI	GCGCGC	1 cut(s)	284
PcsI	WCGNNNNNNNCGW	2 cut(s)	155, 296
PctI	GAATGC	2 cut(s)	1245, 2785
PdiI	GCCGGC	1 cut(s)	273
PdmI	GAANNNNTTC	1 cut(s)	1580
PfeI	GAWTC	4 cut(s)	695, 1640, 2051, 2506
Ple19I	CGATCG	2 cut(s)	105, 505
PluTI	GGCGCC	1 cut(s)	674
Psp1406I	AACGTT	1 cut(s)	1747
Psp6I	CCWGG	3 cut(s)	14, 954, 1020
PspFI	CCCAGC	2 cut(s)	379, 710
PspGI	CCWGG	3 cut(s)	14, 954, 1020
PspN4I	GGNNCC	9 cut(s)	64, 91, 109, 156, 411, 672, 1559, 1848, 2087
PspOMI	GGGCCC	1 cut(s)	154
PspPI	GGNCC	6 cut(s)	154, 155, 410, 1558, 2085, 2638
PspXI	VCTCGAGB	1 cut(s)	939
PstI	CTGCAG	1 cut(s)	2233
PstNI	CAGNNNCTG	2 cut(s)	710, 779
PsuI	RGATCY	3 cut(s)	107, 2731, 2911
PteI	GCGCGC	1 cut(s)	284
PvuI	CGATCG	2 cut(s)	105, 505
PvuII	CAGCTG	1 cut(s)	262
RsaI	GTAC	4 cut(s)	163, 769, 1264, 2614
RsaNI	GTAC	4 cut(s)	162, 768, 1263, 2613
RseI	CAYNNNNRTG	1 cut(s)	1238
SaqAI	TTAA	9 cut(s)	749, 842, 1397, 1626, 1710, 2745, 2754, 3050, 3072
Sau96I	GGNCC	6 cut(s)	154, 155, 410, 1558, 2085, 2638
ScrFI	CCNGG	8 cut(s)	16, 153, 489, 956, 1022, 1212, 1494, 2362
SduI	GDGCHC	4 cut(s)	158, 659, 853, 2491
SfaNI	GCATC	5 cut(s)	607, 632, 1054, 2036, 2452
SfcI	CTRYAG	2 cut(s)	2229, 2649
SfoI	GGCGCC	1 cut(s)	672
Sfr274I	CTCGAG	1 cut(s)	939
SgrAI	CRCCGGYG	1 cut(s)	271
SinI	GGWCC	3 cut(s)	1558, 2085, 2638
SlaI	CTCGAG	1 cut(s)	939
SmiMI	CAYNNNNRTG	1 cut(s)	1238
SmlI	CTYRAG	4 cut(s)	748, 939, 1097, 2431
SmoI	CTYRAG	4 cut(s)	748, 939, 1097, 2431
SspDI	GGCGCC	1 cut(s)	670
SspI	AATATT	1 cut(s)	2999
SspMI	CTAG	7 cut(s)	206, 483, 555, 1266, 1431, 2031, 2247
StyD4I	CCNGG	8 cut(s)	14, 151, 487, 954, 1020, 1210, 1492, 2360
StyI	CCWWGG	2 cut(s)	2246, 2634
TaaI	ACNGT	3 cut(s)	1445, 2473, 2572
TaiI	ACGT	6 cut(s)	666, 1198, 1750, 1933, 2607, 2809
TaqI	TCGA	7 cut(s)	290, 644, 940, 1120, 1237, 1695, 1897
TaqII	GACCGA	1 cut(s)	2655
TatI	WGTACW	2 cut(s)	767, 1262
TauI	GCSGC	5 cut(s)	177, 189, 338, 350, 501
TfiI	GAWTC	4 cut(s)	695, 1640, 2051, 2506
Tru1I	TTAA	9 cut(s)	749, 842, 1397, 1626, 1710, 2745, 2754, 3050, 3072
Tru9I	TTAA	9 cut(s)	749, 842, 1397, 1626, 1710, 2745, 2754, 3050, 3072
TscAI	CASTG	1 cut(s)	1166
TseFI	GTSAC	4 cut(s)	734, 2041, 2078, 2807
TseI	GCWGC	9 cut(s)	542, 606, 620, 1034, 1225, 1273, 2235, 2524, 2940
Tsp45I	GTSAC	4 cut(s)	734, 2041, 2078, 2807
TspRI	CASTG	1 cut(s)	1166
Vha464I	CTTAAG	1 cut(s)	748
VneI	GTGCAC	1 cut(s)	849
VpaK11BI	GGWCC	3 cut(s)	1558, 2085, 2638
XceI	RCATGY	1 cut(s)	2110
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1289
XhoI	CTCGAG	1 cut(s)	939
XmaJI	CCTAGG	1 cut(s)	2246
XmiI	GTMKAC	1 cut(s)	2164
XmnI	GAANNNNTTC	1 cut(s)	1580
XspI	CTAG	7 cut(s)	206, 483, 555, 1266, 1431, 2031, 2247
ZraI	GACGTC	1 cut(s)	1196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.