Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006892

Physical Location & Seq

Genomic Coordinates Forward (+)

36261642 .. 36264550

Gene Structure 2909 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1231500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 825 bp

ATGTTGACAGACTTTGGGCTTTCGAAGGTTGGTCTCATCAACAGCACTGATGATTTATCTGGTCCAGCAGTTAGTAGAACATCCCTTCTAGGGGAAGATGAACCCGAATCATCTCTCTCCGAGCATCAACGAGAAAGCCGAAAGAAACGTTCTGCTGTCGGTACACCTGACTATTTGGCACCAGAGATACTTTTGGGAACAGGACATGGTGCAACTGCAGATTGGTGGTCTGTTGGTGTCATTTTATTTGAATTGATTGTTGGAATTCCACCCTTCAATGCTGAGCACCCTCAGACTATATTTGATAATATTCTCAACCGTAATATACCTTGGCCTGGGGTTGGTGAGATGAGTCCTGAAGCACGAGATCTTATTGATCAGTTATTGACAGAAGATCCTAATCAAAGACTTGGAGCTCGAGGAGCATCAGAGGTGAAGCAGCATCCATTCTTTAAAGACATCAACTGGGACACACTTGCCAGGCAAAAGGCTGCGTTTGTTCCCTCTTCAGAGAGTGCACTAGATACCAGTTACTTCACCAGTCGTTACTCATGGAATCCTTCAGATGAACATGTATATCAACCTAGTGACATGGATGATTCCAGTGATTCTGATAGCTTAAGTGGCAGCAGCAGTTGCTTGAGCAACCGTCATGAGGAAGTGGGTGATGAATGTGGGGGTCTGACAGAGTTCGAGTCTGGCTCTTCTATCAATTACTCTTTCAGTAATTTCTCATTTAAGAATCTCTCCCAGCTTGCATCAATCAACTATGATCTGCTTTCAAAGGGTTTCAAGGATGATCGGTCACTGAATCCCAGCACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

275

Amino Acids

30.09

Weight (kDa)

4.49

Isoelectric Point (pI)

47.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	40 - 151	3.6e-24	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	42 - 137	5.7e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17850
fragaria_vesca	FvH4_5g17560 FvH4_5g17560 FvH4_5g17560 FvH4_5g17560
malus_domestica	MD06G1231400.v1.1 MD06G1231500.v1.1 MD14G1238200.v1.1
prunus_persica	Prupe.5G235400_v2.0.a1
pyrus_communis	pycom06g20530 pycom06g20540 pycom14g19930 pycom14g19940
rosa_chinensis	RchiOBHm_Chr7g0178091
rosa_laevigata	RLG00000005472
rosa_multiflora	Rmu_co8223356.1_g000001 Rmu_sc0000425.1_g000008
rosa_roxburghii	Rroxscaffold_3G00275190
rosa_rugosa	Rorug06G0413400 Rorug06G0413500 Rorug06G0413500
rosa_samantha	Rh7AG012700 Rh7BG012600 Rh7CG012600 Rh7DG012900
rosa_wichuraiana	Rw7G001000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	178
AclI	AACGTT	1 cut(s)	148
AclWI	GGATC	1 cut(s)	389
AcsI	RAATTY	1 cut(s)	264
AcuI	CTGAAG	3 cut(s)	378, 492, 546
AfaI	GTAC	1 cut(s)	163
AfiI	CCNNNNNNNGG	5 cut(s)	90, 91, 335, 341, 655
AflII	CTTAAG	1 cut(s)	619
AflIII	ACRYGT	1 cut(s)	571
AgsI	TTSAA	4 cut(s)	251, 277, 783, 793
AjnI	CCWGG	2 cut(s)	334, 479
AjuI	GAANNNNNNNTTGG	2 cut(s)	243, 275
AluBI	AGCT	3 cut(s)	416, 618, 754
AluI	AGCT	3 cut(s)	416, 618, 754
Alw21I	GWGCWC	3 cut(s)	288, 418, 520
Alw26I	GTCTC	1 cut(s)	38
Alw44I	GTGCAC	1 cut(s)	516
AlwI	GGATC	1 cut(s)	389
Ama87I	CYCGRG	1 cut(s)	417
AoxI	GGCC	1 cut(s)	332
ApaLI	GTGCAC	1 cut(s)	516
ApeKI	GCWGC	4 cut(s)	439, 491, 627, 630
ApoI	RAATTY	1 cut(s)	264
AspS9I	GGNCC	1 cut(s)	62
AsuHPI	GGTGA	4 cut(s)	356, 445, 529, 677
AsuII	TTCGAA	1 cut(s)	23
AvaI	CYCGRG	1 cut(s)	417
AvaII	GGWCC	1 cut(s)	62
BaeGI	GKGCMC	1 cut(s)	520
BanI	GGYRCC	1 cut(s)	178
BanII	GRGCYC	1 cut(s)	418
BauI	CACGAG	1 cut(s)	363
Bbv12I	GWGCWC	3 cut(s)	288, 418, 520
BbvI	GCAGC	4 cut(s)	451, 478, 639, 642
BciT130I	CCWGG	2 cut(s)	336, 481
BclI	TGATCA	1 cut(s)	376
BcoDI	GTCTC	1 cut(s)	38
BfaI	CTAG	3 cut(s)	89, 521, 585
BfmI	CTRYAG	1 cut(s)	216
BfrI	CTTAAG	1 cut(s)	619
BglII	AGATCT	1 cut(s)	367
BisI	GCNGC	4 cut(s)	440, 492, 628, 631
BlpI	GCTNAGC	1 cut(s)	282
BlsI	GCNGC	4 cut(s)	441, 493, 629, 632
Bme1390I	CCNGG	2 cut(s)	336, 481
Bme18I	GGWCC	1 cut(s)	62
BmeT110I	CYCGRG	1 cut(s)	417
BmgT120I	GGNCC	1 cut(s)	62
BmiI	GGNNCC	1 cut(s)	180
BmrFI	CCNGG	2 cut(s)	336, 481
BmrI	ACTGGG	1 cut(s)	475
BmsI	GCATC	4 cut(s)	133, 434, 451, 767
BmuI	ACTGGG	1 cut(s)	475
BplI	GAGNNNNNCTC	2 cut(s)	686, 718
Bpu1102I	GCTNAGC	1 cut(s)	282
Bpu14I	TTCGAA	1 cut(s)	23
BpuEI	CTTGAG	1 cut(s)	661
BsaAI	YACGTR	1 cut(s)	822
BsaBI	GATNNNNATC	1 cut(s)	399
BsaI	GGTCTC	1 cut(s)	38
BsaJI	CCNNGG	2 cut(s)	329, 335
Bsc4I	CCNNNNNNNGG	5 cut(s)	90, 91, 335, 341, 655
Bse1I	ACTGG	4 cut(s)	470, 528, 540, 603
Bse8I	GATNNNNATC	1 cut(s)	399
BseBI	CCWGG	2 cut(s)	336, 481
BseDI	CCNNGG	2 cut(s)	329, 335
BseGI	GGATG	4 cut(s)	80, 442, 601, 802
BseJI	GATNNNNATC	1 cut(s)	399
BseLI	CCNNNNNNNGG	5 cut(s)	90, 91, 335, 341, 655
BseMII	CTCAG	2 cut(s)	273, 305
BseNI	ACTGG	4 cut(s)	470, 528, 540, 603
BseRI	GAGGAG	1 cut(s)	435
BseSI	GKGCMC	1 cut(s)	520
BseXI	GCAGC	4 cut(s)	451, 478, 639, 642
BseYI	CCCAGC	2 cut(s)	750, 815
BshFI	GGCC	1 cut(s)	334
BshNI	GGYRCC	1 cut(s)	178
BsiHKAI	GWGCWC	3 cut(s)	288, 418, 520
BsiHKCI	CYCGRG	1 cut(s)	417
BslFI	GGGAC	1 cut(s)	482
BslI	CCNNNNNNNGG	5 cut(s)	90, 91, 335, 341, 655
BsmAI	GTCTC	1 cut(s)	38
BsmFI	GGGAC	1 cut(s)	482
BsnI	GGCC	1 cut(s)	334
Bso31I	GGTCTC	1 cut(s)	38
BsoBI	CYCGRG	1 cut(s)	417
Bsp119I	TTCGAA	1 cut(s)	23
Bsp1286I	GDGCHC	3 cut(s)	288, 418, 520
Bsp143I	GATC	5 cut(s)	367, 376, 394, 772, 799
Bsp1720I	GCTNAGC	1 cut(s)	282
BspANI	GGCC	1 cut(s)	334
BspCNI	CTCAG	2 cut(s)	274, 304
BspHI	TCATGA	1 cut(s)	652
BspLI	GGNNCC	1 cut(s)	180
BspMAI	CTGCAG	1 cut(s)	220
BspPI	GGATC	1 cut(s)	389
BspQI	GCTCTTC	1 cut(s)	709
BspT104I	TTCGAA	1 cut(s)	23
BspT107I	GGYRCC	1 cut(s)	178
BspTI	CTTAAG	1 cut(s)	619
BspTNI	GGTCTC	1 cut(s)	38
BsrI	ACTGG	4 cut(s)	470, 528, 540, 603
BssECI	CCNNGG	2 cut(s)	329, 335
BssMI	GATC	5 cut(s)	367, 376, 394, 772, 799
BssSI	CACGAG	1 cut(s)	363
BssT1I	CCWWGG	1 cut(s)	329
Bst2BI	CACGAG	1 cut(s)	363
Bst2UI	CCWGG	2 cut(s)	336, 481
Bst4CI	ACNGT	2 cut(s)	320, 650
Bst6I	CTCTTC	2 cut(s)	511, 709
BstAFI	CTTAAG	1 cut(s)	619
BstAPI	GCANNNNNTGC	1 cut(s)	636
BstBAI	YACGTR	1 cut(s)	822
BstBI	TTCGAA	1 cut(s)	23
BstC8I	GCNNGC	1 cut(s)	756
BstDEI	CTNAG	2 cut(s)	282, 291
BstF5I	GGATG	4 cut(s)	80, 442, 601, 802
BstKTI	GATC	5 cut(s)	370, 379, 397, 775, 802
BstMAI	GTCTC	1 cut(s)	38
BstMBI	GATC	5 cut(s)	367, 376, 394, 772, 799
BstMWI	GCNNNNNNNGC	3 cut(s)	422, 624, 636
BstNI	CCWGG	2 cut(s)	336, 481
BstNSI	RCATGY	1 cut(s)	575
BstSCI	CCNGG	2 cut(s)	334, 479
BstSFI	CTRYAG	1 cut(s)	216
BstSLI	GKGCMC	1 cut(s)	520
BstV1I	GCAGC	4 cut(s)	451, 478, 639, 642
BstX2I	RGATCY	2 cut(s)	367, 394
BstYI	RGATCY	2 cut(s)	367, 394
BsuRI	GGCC	1 cut(s)	334
BtsCI	GGATG	4 cut(s)	80, 442, 601, 802
BtsIMutI	CAGTG	3 cut(s)	45, 610, 806
Cac8I	GCNNGC	1 cut(s)	756
CciI	TCATGA	1 cut(s)	652
Cfr13I	GGNCC	1 cut(s)	62
Csp6I	GTAC	1 cut(s)	162
CviAII	CATG	5 cut(s)	206, 552, 572, 592, 653
CviJI	RGCY	8 cut(s)	19, 138, 334, 416, 491, 618, 702, 754
CviKI_1	RGCY	8 cut(s)	19, 138, 334, 416, 491, 618, 702, 754
CviQI	GTAC	1 cut(s)	162
DdeI	CTNAG	2 cut(s)	282, 291
DpnI	GATC	5 cut(s)	369, 378, 396, 774, 801
DpnII	GATC	5 cut(s)	367, 376, 394, 772, 799
DraI	TTTAAA	1 cut(s)	454
Eam1104I	CTCTTC	2 cut(s)	511, 709
EarI	CTCTTC	2 cut(s)	511, 709
Ecl136II	GAGCTC	1 cut(s)	416
Eco130I	CCWWGG	1 cut(s)	329
Eco24I	GRGCYC	1 cut(s)	418
Eco31I	GGTCTC	1 cut(s)	38
Eco47I	GGWCC	1 cut(s)	62
Eco53kI	GAGCTC	1 cut(s)	416
Eco57I	CTGAAG	3 cut(s)	378, 492, 546
Eco88I	CYCGRG	1 cut(s)	417
EcoICRI	GAGCTC	1 cut(s)	416
EcoRI	GAATTC	1 cut(s)	264
EcoRII	CCWGG	2 cut(s)	334, 479
EcoT14I	CCWWGG	1 cut(s)	329
EcoT38I	GRGCYC	1 cut(s)	418
ErhI	CCWWGG	1 cut(s)	329
FaeI	CATG	5 cut(s)	209, 555, 575, 595, 656
FaiI	YATR	9 cut(s)	207, 299, 326, 553, 573, 577, 593, 654, 771
FaqI	GGGAC	1 cut(s)	482
FatI	CATG	5 cut(s)	205, 551, 571, 591, 652
FbaI	TGATCA	1 cut(s)	376
Fnu4HI	GCNGC	4 cut(s)	440, 492, 628, 631
FokI	GGATG	4 cut(s)	67, 429, 608, 809
FriOI	GRGCYC	1 cut(s)	418
Fsp4HI	GCNGC	4 cut(s)	440, 492, 628, 631
FspBI	CTAG	3 cut(s)	89, 521, 585
GluI	GCNGC	4 cut(s)	440, 492, 628, 631
GsaI	CCCAGC	2 cut(s)	754, 819
HaeIII	GGCC	1 cut(s)	334
Hin1II	CATG	5 cut(s)	209, 555, 575, 595, 656
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HinfI	GANTC	8 cut(s)	107, 352, 556, 599, 608, 695, 742, 811
HphI	GGTGA	4 cut(s)	356, 445, 529, 677
Hpy166II	GTNNAC	3 cut(s)	6, 164, 518
Hpy188I	TCNGA	7 cut(s)	121, 294, 430, 511, 565, 613, 684
Hpy188III	TCNNGA	2 cut(s)	356, 653
Hpy8I	GTNNAC	3 cut(s)	6, 164, 518
HpyAV	CCTTC	4 cut(s)	19, 95, 283, 570
HpyCH4III	ACNGT	2 cut(s)	320, 650
HpyCH4IV	ACGT	2 cut(s)	148, 821
HpyCH4V	TGCA	4 cut(s)	212, 218, 518, 758
HpyF10VI	GCNNNNNNNGC	3 cut(s)	422, 624, 636
HpyF3I	CTNAG	2 cut(s)	282, 291
HpySE526I	ACGT	2 cut(s)	148, 821
Hsp92II	CATG	5 cut(s)	209, 555, 575, 595, 656
Ksp22I	TGATCA	1 cut(s)	376
Kzo9I	GATC	5 cut(s)	367, 376, 394, 772, 799
LguI	GCTCTTC	1 cut(s)	709
LmnI	GCTCC	2 cut(s)	413, 422
Lsp1109I	GCAGC	4 cut(s)	451, 478, 639, 642
LweI	GCATC	4 cut(s)	133, 434, 451, 767
MaeI	CTAG	3 cut(s)	89, 521, 585
MaeII	ACGT	2 cut(s)	148, 821
MaeIII	GTNAC	4 cut(s)	530, 545, 587, 804
MalI	GATC	5 cut(s)	369, 378, 396, 774, 801
MboI	GATC	5 cut(s)	367, 376, 394, 772, 799
MboII	GAAGA	4 cut(s)	107, 404, 498, 696
MflI	RGATCY	2 cut(s)	367, 394
MhlI	GDGCHC	3 cut(s)	288, 418, 520
MluCI	AATT	4 cut(s)	251, 264, 712, 727
MlyI	GAGTC	2 cut(s)	361, 704
MmeI	TCCRAC	1 cut(s)	241
MnlI	CCTC	5 cut(s)	300, 413, 424, 514, 649
MseI	TTAA	3 cut(s)	453, 620, 738
MspCI	CTTAAG	1 cut(s)	619
MspR9I	CCNGG	2 cut(s)	336, 481
MvaI	CCWGG	2 cut(s)	336, 481
MwoI	GCNNNNNNNGC	3 cut(s)	422, 624, 636
NdeII	GATC	5 cut(s)	367, 376, 394, 772, 799
NlaIII	CATG	5 cut(s)	209, 555, 575, 595, 656
NlaIV	GGNNCC	1 cut(s)	180
NmuCI	GTSAC	2 cut(s)	587, 804
NspI	RCATGY	1 cut(s)	575
NspV	TTCGAA	1 cut(s)	23
PaeR7I	CTCGAG	1 cut(s)	417
PagI	TCATGA	1 cut(s)	652
PciI	ACATGT	1 cut(s)	571
PciSI	GCTCTTC	1 cut(s)	709
PcsI	WCGNNNNNNNCGW	1 cut(s)	136
PfeI	GAWTC	6 cut(s)	107, 556, 599, 608, 742, 811
PkrI	GCNGC	4 cut(s)	441, 493, 629, 632
PleI	GAGTC	2 cut(s)	360, 703
PpsI	GAGTC	2 cut(s)	360, 703
Ppu21I	YACGTR	1 cut(s)	822
PscI	ACATGT	1 cut(s)	571
Psp124BI	GAGCTC	1 cut(s)	418
Psp1406I	AACGTT	1 cut(s)	148
Psp6I	CCWGG	2 cut(s)	334, 479
PspFI	CCCAGC	2 cut(s)	750, 815
PspGI	CCWGG	2 cut(s)	334, 479
PspN4I	GGNNCC	1 cut(s)	180
PspPI	GGNCC	1 cut(s)	62
PspXI	VCTCGAGB	1 cut(s)	417
PstI	CTGCAG	1 cut(s)	220
PsuI	RGATCY	2 cut(s)	367, 394
RsaI	GTAC	1 cut(s)	163
RsaNI	GTAC	1 cut(s)	162
SacI	GAGCTC	1 cut(s)	418
SapI	GCTCTTC	1 cut(s)	709
SaqAI	TTAA	3 cut(s)	453, 620, 738
SatI	GCNGC	4 cut(s)	440, 492, 628, 631
Sau3AI	GATC	5 cut(s)	367, 376, 394, 772, 799
Sau96I	GGNCC	1 cut(s)	62
SchI	GAGTC	2 cut(s)	361, 704
ScrFI	CCNGG	2 cut(s)	336, 481
SduI	GDGCHC	3 cut(s)	288, 418, 520
SfaNI	GCATC	4 cut(s)	133, 434, 451, 767
SfcI	CTRYAG	1 cut(s)	216
Sfr274I	CTCGAG	1 cut(s)	417
SfuI	TTCGAA	1 cut(s)	23
SinI	GGWCC	1 cut(s)	62
SlaI	CTCGAG	1 cut(s)	417
SmlI	CTYRAG	3 cut(s)	417, 619, 640
SmoI	CTYRAG	3 cut(s)	417, 619, 640
Sse9I	AATT	4 cut(s)	251, 264, 712, 727
SspI	AATATT	1 cut(s)	310
SspMI	CTAG	3 cut(s)	89, 521, 585
SstI	GAGCTC	1 cut(s)	418
StyD4I	CCNGG	2 cut(s)	334, 479
StyI	CCWWGG	1 cut(s)	329
TaaI	ACNGT	2 cut(s)	320, 650
TaiI	ACGT	2 cut(s)	151, 824
TaqI	TCGA	3 cut(s)	23, 418, 693
TaqII	GACCGA	1 cut(s)	792
TasI	AATT	4 cut(s)	251, 264, 712, 727
TfiI	GAWTC	6 cut(s)	107, 556, 599, 608, 742, 811
Tru1I	TTAA	3 cut(s)	453, 620, 738
Tru9I	TTAA	3 cut(s)	453, 620, 738
TscAI	CASTG	3 cut(s)	52, 610, 813
TseFI	GTSAC	2 cut(s)	587, 804
TseI	GCWGC	4 cut(s)	439, 491, 627, 630
Tsp45I	GTSAC	2 cut(s)	587, 804
TspDTI	ATGAA	3 cut(s)	114, 582, 684
TspRI	CASTG	3 cut(s)	52, 610, 813
Vha464I	CTTAAG	1 cut(s)	619
VneI	GTGCAC	1 cut(s)	516
VpaK11BI	GGWCC	1 cut(s)	62
XapI	RAATTY	1 cut(s)	264
XceI	RCATGY	1 cut(s)	575
XhoI	CTCGAG	1 cut(s)	417
XspI	CTAG	3 cut(s)	89, 521, 585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.