Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006892

Physical Location & Seq

Genomic Coordinates Forward (+)

31739642 .. 31748471

Gene Structure 8830 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1238200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3891 bp

ATGGTGTTCAAGGGCAGGTTCTTCTCTTCCAAGAAATCCGACTCCTCCAACAGCCCCGATGGGTCGAATAACAGCCCGCGATCTCTGGGTTCCAACTCCCCGATCCGATCCGATAAGAAGAAGCCTAAATCCGCATCCAAAGACGACTCGCCCGGCCCGAGTAAAAGTTCCGGCGGCGGCGGCGGATTCAGTAGGCAGAGTCTAGTCAAAGACGGGTCAAAGAAGAAGGATGCGAGGGGGAAAGAGAGTCCAGCTCAGCTTAAGACGCCGGCGAAGTCCGCGAGCTCGACTGGCGCGACTCCGAAGAAGTCGTCTGCGTCCGCATCGGGGGCTGCGGAACCGGCGGCTTCGGTGTCTCCCATTCTGGCGTCGTCGCTGGGGTTGAATAGGATAAAGACGAGATCGGGGCCGTTGCCTCAGGAGAGCTTTTTTGGGTTTAGGGCTGACAAAGGGTCGGCGCTGGGCTCGAGTAATTTGTCGAGGCCGGGGGTCGGCGATCGGAGTTTAGGATCGGGAAGTGGGGGGAAGAAGAAGGAGGCTGCGAGTCAGAGTAGGATTGGGTTCAATGACAATGTGGCAAGTGGGAGTTGGGGTGATAATGGCAGCAATTCTGATGCTGCCTCAACTGGTAGCTTGCCTTCGAGAGACCAGAGCCCCAACGGGGTGGCACCTTCGCGGTTACGCAAGGGGGAATCTTCGGCTGAAGACGGGAGAAACATATCATCTTTTGGTCACTCTGGAGGTTTAAGAAGTTCAGATGTATGTACACCAGAGACTGCATATGATTGTGAAAATCCCAAGGAGTCCGAGTCGCCCCGTTTTCAAGCTATACTCCGCTTAACGAGTGCACCAAGAAAGCGGTTTCCTGCTGATATAAAAAGTTTTTCCCATGAACTGAATTCAAAAGGTGTACGACCTTTCCCATTTTGGAAGCCCCGAGGCTTGAACAACCTGGAGGAAATCTTGGTTGCAATTAGGGCAAAGTTTGACAAAGCAAAGGAAGAAGTGAACTCTGATCTGGCTATATTTGCAGCAGACTTAGTTGGAATTCTTGAAAAAGATGCAGATAATCATCCAGAGTTGCAGGAAACTCTTGAGGATTTGCTGGTTTTGGCACGAAGTTGTGCAATGACATCATCTGGAGAGTTTTGGCTTCAATGTGAAGGCATAGTTCAGGAATTGGATGATAGACGTCAAGAACTTCCTCCGGGTGTGCTGAAGCAGCTTCACACTCGAATGCTTTTCATACTCACAAGGTGTACTAGGTTGCTGCAATTCCATAAGGAAACCGGACTGGCTGAGGATGAACACGTTTTCCAGCTTCGTCAATCAAGAGTTCTGCGTTCCACTGATAAAAGAATTCCGCCAAGTTTGGCAAAGGATCCAAAGAGCTTAAGTGTTGCAAAAGCCTCTAAAGCAGCTTCAGCTAGGAAATCTTACAGTCAGGAGCAACACGGTTTGGAATGGAAGAGAGACCATGTTTTATCGGGAAATTTGTTCTCACCTCTTGTTGATCAACCACCTAAGAATTCGGAGTCTCCTGCTAGTAGGGACCGGATGACATCTTGGAAGAAATTCCCATCTCCGGTTGCGAAAAGCTTGAAAGAAACTACTGAATTTAAGGAGCAGAGTGATCCTAAGGTTGAACATTTGAAAGGTTCAGACAACAAGAGAGGGACTTCTGACATTGATCCTACTTCTATTAAGCTTCCTGAGCCTTCTGGCAAGGATTCTCATGAGCGTTCTTCCAAGCACCAGCACAAACCGTCTTGGGGTTGGGGAAATCAACCGAATGTATCTGACGATAGTTCTATAATATGTCGCATCTGTGAGGAGGAGGTTCCAACTGCAAATGTGGAAGACCATTCAAGAATTTGTGCGATTGCTGATCGATGTGATCCAAAGGGTATAAGTGTGAATGAGCGTCTTGTCAGAATTTCTGAAACCCTTGAAAAACTGATGGAGTCCTTTACTCAAAAGGATATCCAGCATGGTGTGGGAAGTCCAGACGTTGCAAAAGCATCTAATTCAAGTGTGACCGAAGAATCTGACGTTATATCTCCAAAACTTAGTGACTGGTCCCACAGAGGCTCAGAGGACATGCTCGATTGTTTTCCTGAAGCTGATAATTCAGCTTTTATGGATGACCTGAAAGGTCTACCTTCCATGTCATGTAGAACTCGTTTTGGTCCAAAGTCTGATCAAGGAATGACAACATCATCTGCAGGCAGCATGACTCCTAGGTCTCCCTTATTGACACCAAGGGCCAGTCAGATTGACATGCTCCTCGCAGGCAAGGCTTCTTTCTCTGAGCAAGACGATATTCCTCAGATGAATGAACTTTCTGATATTGCTCGGTGCGTAGCAAATATGCCTTTGGATGATGATCGCTCTATGCCATATCTACTGTCTTGTCTTGAAGACTTGAGAGTTGTTATAGACCGCAGAAAGTTTGATGCACTTACCGTCGAAACATTTGGGGCACGCATTGAGAAGCTGATTCGGGAGAAGTATTTGCAGCTTTGTGAATTAGTGGATGATGAGAAGGTTGATTTAGCAACTACCGTAATTGATGAAGAAGCTCCACTAGAAGATGACGTGGTTCGTACAAGCCCTATACCGTTTTCCAAGGACCGAACATCTATAGATGACTTTGAAATTATCAAGCCAATAAGTCGTGGGGCATTTGGGCGAGTTTTCTTAGCTAAAAAGAGAACAACAGGGGATCTTTTTGCGATTAAGGTTCTTAAAAAGGCTGATATGATCCGGAAGAATGCTGTTGAAAGTATTTTAGCAGAACGTGACATCTTGATTTCAGTCCGCAATCCTTTTGTGGTTCGCTTCTTCTATTCTTTTACTTGTAGTGAAAACTTGTATCTTGTGATGGAGTATTTGAACGGAGGAGATCTATACTCATTGTTGAGAAATTTAGGCTGCTTGGATGAGGAGGTTGCTCGTGTATATATAGCAGAAGTGGTGCTTGCTTTAGAGTATTTACATTCACTTCGTGTGGTTCATCGTGATTTGAAGCCTGACAATTTGTTAATTGCTCATGACGGCCATGTTAAGTTGACAGACTTTGGGCTTTCGAAGGTTGGTCTCATCAACAGCACTGATGATTTATCTGGTCCAGCAGTTAGTAGCACATCCCTTCTAGGGGAAGATGAACCCGAATTATCTCTCTCTGAGCATCAGCGAGAAAGCCGAAAGAAACGTTCTGCAGTCGGTACACCTGACTATTTGGCACCAGAGATACTTTTGGGAACAGGACATGGTGCAACTGCAGATTGGTGGTCTGTTGGTGTCATTTTATTTGAATTGATTGTTGGAATTCCACCTTTCAATGCTGAGCATCCTCAGACTATATTTGATAATATTCTCAACCGTAATATTCCTTGGCCTGGGGTTGGCGAGATGAGTCCTGAAGCACAGGATCTTATTGATCAGTTATTGACAGAAGATCCTAATCAAAGACTTGGAGCTAGAGGTGCATCAGAGGTGAAGCAGCATCCATTCTTTAAAGATATCAACTGGGACACGCTTGCCAGGCAAAAGGCTGCGTTTGTTCCCACTTCAGAGAGTGCTCTAGATACCAGTTACTTCACCAGTCGTTTCTCATGGAATCCTTCAGATCAACATGTATATCCACCCAGTGACATGGATGATTCCAGTGATTCTGACAGCTTAAGTGGCAGCAGCAGTTGCTTGAGCAACCGTCATGAGGAAGTGGGTGATGAATGTGGGGGTCTGACAGAGTTCGAGTCTGGCTCTAATATCAATTACTCTTTCAGTAATTTCTCATTTAAGAATCTCTCCCAGCTTGCATCAATCAACTATGATCTGCTTTCAAAGGGTTTTAAGGATGATCCGTCATTGAATCCCAGCGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1297

Amino Acids

141.9

Weight (kDa)

5.74

Isoelectric Point (pI)

50.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	310 - 428	2.3e-55	IREH1-like domain
Pkinase	PF00069	885 - 1173	1.6e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	886 - 1032	3.5e-25	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17850
fragaria_vesca	FvH4_5g17560 FvH4_5g17560 FvH4_5g17560 FvH4_5g17560
malus_domestica	MD06G1231400.v1.1 MD06G1231500.v1.1 MD14G1238200.v1.1
prunus_persica	Prupe.5G235400_v2.0.a1
pyrus_communis	pycom06g20530 pycom06g20540 pycom14g19930 pycom14g19940
rosa_chinensis	RchiOBHm_Chr7g0178091
rosa_laevigata	RLG00000005472
rosa_multiflora	Rmu_co8223356.1_g000001 Rmu_sc0000425.1_g000008
rosa_roxburghii	Rroxscaffold_3G00275190
rosa_rugosa	Rorug06G0413400 Rorug06G0413500 Rorug06G0413500
rosa_samantha	Rh7AG012700 Rh7BG012600 Rh7CG012600 Rh7DG012900
rosa_wichuraiana	Rw7G001000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2242
AatII	GACGTC	1 cut(s)	1195
Acc36I	ACCTGC	1 cut(s)	6
AccB1I	GGYRCC	2 cut(s)	667, 3244
AccI	GTMKAC	1 cut(s)	2158
AccII	CGCG	4 cut(s)	79, 281, 296, 676
AccIII	TCCGGA	1 cut(s)	2766
AclI	AACGTT	1 cut(s)	3214
AcoI	YGGCCR	1 cut(s)	3058
AcuI	CTGAAG	7 cut(s)	723, 1238, 1407, 2139, 3444, 3558, 3612
AcyI	GRCGYC	3 cut(s)	266, 368, 1192
AdeI	CACNNNGTG	3 cut(s)	1257, 3008, 3653
AfaI	GTAC	5 cut(s)	766, 912, 1261, 2608, 3229
AflII	CTTAAG	3 cut(s)	260, 1393, 3685
AflIII	ACRYGT	2 cut(s)	1309, 3637
AhdI	GACNNNNNGTC	1 cut(s)	451
AjiI	CACGTC	1 cut(s)	2599
AjnI	CCWGG	3 cut(s)	951, 3400, 3545
AjuI	GAANNNNNNNTTGG	2 cut(s)	3309, 3341
AloI	GAACNNNNNNTCC	2 cut(s)	1299, 1331
Alw21I	GWGCWC	3 cut(s)	287, 850, 3586
Alw26I	GTCTC	7 cut(s)	360, 639, 767, 1467, 1542, 2250, 3104
Alw44I	GTGCAC	1 cut(s)	846
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	3 cut(s)	157, 466, 936
Aor13HI	TCCGGA	1 cut(s)	2766
AoxI	GGCC	6 cut(s)	154, 407, 482, 2265, 3058, 3398
ApaLI	GTGCAC	1 cut(s)	846
ArsI	GACNNNNNNTTYG	2 cut(s)	296, 328
Asp700I	GAANNNNTTC	1 cut(s)	1574
AspA2I	CCTAGG	1 cut(s)	2240
AspLEI	GCGC	3 cut(s)	296, 460, 3887
AspS9I	GGNCC	8 cut(s)	155, 407, 1552, 2079, 2189, 2265, 2632, 3128
AsuC2I	CCSGG	3 cut(s)	153, 486, 1209
AsuHPI	GGTGA	5 cut(s)	605, 1494, 3511, 3595, 3743
AsuII	TTCGAA	1 cut(s)	3089
AvaI	CYCGRG	3 cut(s)	157, 466, 936
AvaII	GGWCC	5 cut(s)	1552, 2079, 2189, 2632, 3128
AvrII	CCTAGG	1 cut(s)	2240
AxyI	CCTNAGG	2 cut(s)	417, 1638
BaeGI	GKGCMC	2 cut(s)	850, 2485
BamHI	GGATCC	1 cut(s)	1381
BanI	GGYRCC	2 cut(s)	667, 3244
BanII	GRGCYC	3 cut(s)	287, 467, 656
BarI	GAAGNNNNNNTAC	2 cut(s)	622, 654
BauI	CACGAG	1 cut(s)	2955
BbsI	GAAGAC	3 cut(s)	711, 1866, 2427
Bbv12I	GWGCWC	3 cut(s)	287, 850, 3586
BbvCI	CCTCAGC	1 cut(s)	1299
BccI	CCATC	4 cut(s)	53, 1588, 1954, 2878
BceAI	ACGGC	2 cut(s)	394, 3073
BciT130I	CCWGG	3 cut(s)	953, 3402, 3547
BclI	TGATCA	3 cut(s)	1513, 2200, 3442
BcnI	CCSGG	3 cut(s)	153, 486, 1209
BcoDI	GTCTC	7 cut(s)	360, 639, 767, 1467, 1542, 2250, 3104
BfaI	CTAG	9 cut(s)	203, 1263, 1428, 1545, 2241, 2588, 3155, 3483, 3587
BfmI	CTRYAG	4 cut(s)	2223, 2643, 3219, 3282
BfoI	RGCGCY	1 cut(s)	461
BfrI	CTTAAG	3 cut(s)	260, 1393, 3685
BfuAI	ACCTGC	1 cut(s)	6
BglII	AGATCT	1 cut(s)	2905
BlnI	CCTAGG	1 cut(s)	2240
BlpI	GCTNAGC	2 cut(s)	255, 3348
Bme1390I	CCNGG	6 cut(s)	153, 486, 953, 1209, 3402, 3547
Bme18I	GGWCC	5 cut(s)	1552, 2079, 2189, 2632, 3128
BmeRI	GACNNNNNGTC	1 cut(s)	451
BmeT110I	CYCGRG	3 cut(s)	157, 466, 936
BmgBI	CACGTC	1 cut(s)	2599
BmgT120I	GGNCC	8 cut(s)	155, 407, 1552, 2079, 2189, 2265, 2632, 3128
BmiI	GGNNCC	9 cut(s)	91, 339, 408, 669, 1383, 1553, 1842, 2081, 3246
BmrFI	CCNGG	6 cut(s)	153, 486, 953, 1209, 3402, 3547
BmrI	ACTGGG	2 cut(s)	3541, 3645
BmuI	ACTGGG	2 cut(s)	3541, 3645
BpiI	GAAGAC	3 cut(s)	711, 1866, 2427
BplI	GAGNNNNNCTC	4 cut(s)	238, 270, 3752, 3784
BpmI	CTGGAG	3 cut(s)	759, 974, 1161
Bpu10I	CCTNAGC	2 cut(s)	1299, 1713
Bpu1102I	GCTNAGC	2 cut(s)	255, 3348
Bpu14I	TTCGAA	1 cut(s)	3089
BpuEI	CTTGAG	3 cut(s)	1115, 2446, 3727
BpuMI	CCSGG	3 cut(s)	153, 486, 1209
Bsa29I	ATCGAT	1 cut(s)	1891
BsaBI	GATNNNNATC	3 cut(s)	1071, 2385, 3465
BsaHI	GRCGYC	3 cut(s)	266, 368, 1192
BsaI	GGTCTC	4 cut(s)	639, 1467, 2250, 3104
BsaJI	CCNNGG	8 cut(s)	485, 798, 937, 2240, 2261, 2628, 3395, 3401
BsaWI	WCCGGW	4 cut(s)	1289, 1554, 1585, 2766
BsaXI	ACNNNNNCTCC	4 cut(s)	577, 607, 794, 824
Bse118I	RCCGGY	2 cut(s)	268, 340
Bse21I	CCTNAGG	2 cut(s)	417, 1638
Bse3DI	GCAATG	1 cut(s)	1134
Bse8I	GATNNNNATC	3 cut(s)	1071, 2385, 3465
BseAI	TCCGGA	1 cut(s)	2766
BseBI	CCWGG	3 cut(s)	953, 3402, 3547
BseCI	ATCGAT	1 cut(s)	1891
BseDI	CCNNGG	8 cut(s)	485, 798, 937, 2240, 2261, 2628, 3395, 3401
BseJI	GATNNNNATC	3 cut(s)	1071, 2385, 3465
BseMI	GCAATG	1 cut(s)	1134
BseRI	GAGGAG	6 cut(s)	34, 1847, 1850, 2276, 2916, 2960
BseSI	GKGCMC	2 cut(s)	850, 2485
BseYI	CCCAGC	4 cut(s)	376, 460, 3816, 3881
Bsh1236I	CGCG	4 cut(s)	79, 281, 296, 676
Bsh1285I	CGRYCG	1 cut(s)	499
BshFI	GGCC	6 cut(s)	156, 409, 484, 2267, 3060, 3400
BshNI	GGYRCC	2 cut(s)	667, 3244
BshVI	ATCGAT	1 cut(s)	1891
BsiEI	CGRYCG	1 cut(s)	499
BsiHKAI	GWGCWC	3 cut(s)	287, 850, 3586
BsiHKCI	CYCGRG	3 cut(s)	157, 466, 936
BslFI	GGGAC	4 cut(s)	1565, 1690, 2065, 3548
BsmAI	GTCTC	7 cut(s)	360, 639, 767, 1467, 1542, 2250, 3104
BsmFI	GGGAC	4 cut(s)	1565, 1690, 2065, 3548
BsmI	GAATGC	2 cut(s)	1242, 2779
BsnI	GGCC	6 cut(s)	156, 409, 484, 2267, 3060, 3400
Bso31I	GGTCTC	4 cut(s)	639, 1467, 2250, 3104
BsoBI	CYCGRG	3 cut(s)	157, 466, 936
Bsp119I	TTCGAA	1 cut(s)	3089
Bsp1286I	GDGCHC	6 cut(s)	287, 467, 656, 850, 2485, 3586
Bsp13I	TCCGGA	1 cut(s)	2766
Bsp1407I	TGTACA	1 cut(s)	764
Bsp1720I	GCTNAGC	2 cut(s)	255, 3348
BspANI	GGCC	6 cut(s)	156, 409, 484, 2267, 3060, 3400
BspDI	ATCGAT	1 cut(s)	1891
BspEI	TCCGGA	1 cut(s)	2766
BspFNI	CGCG	4 cut(s)	79, 281, 296, 676
BspHI	TCATGA	3 cut(s)	1735, 3052, 3718
BspLI	GGNNCC	9 cut(s)	91, 339, 408, 669, 1383, 1553, 1842, 2081, 3246
BspMAI	CTGCAG	3 cut(s)	2227, 3223, 3286
BspMI	ACCTGC	1 cut(s)	6
BspT104I	TTCGAA	1 cut(s)	3089
BspT107I	GGYRCC	2 cut(s)	667, 3244
BspTI	CTTAAG	3 cut(s)	260, 1393, 3685
BspTNI	GGTCTC	4 cut(s)	639, 1467, 2250, 3104
BsrDI	GCAATG	1 cut(s)	1134
BsrFI	RCCGGY	2 cut(s)	268, 340
BsrGI	TGTACA	1 cut(s)	764
BssAI	RCCGGY	2 cut(s)	268, 340
BssECI	CCNNGG	8 cut(s)	485, 798, 937, 2240, 2261, 2628, 3395, 3401
BssNI	GRCGYC	3 cut(s)	266, 368, 1192
BssSI	CACGAG	1 cut(s)	2955
BssT1I	CCWWGG	5 cut(s)	798, 2240, 2261, 2628, 3395
Bst2BI	CACGAG	1 cut(s)	2955
Bst2UI	CCWGG	3 cut(s)	953, 3402, 3547
Bst4CI	ACNGT	9 cut(s)	1442, 1457, 1767, 2409, 2467, 2566, 2622, 3386, 3716
Bst6I	CTCTTC	2 cut(s)	31, 1463
BstACI	GRCGYC	3 cut(s)	266, 368, 1192
BstAFI	CTTAAG	3 cut(s)	260, 1393, 3685
BstAPI	GCANNNNNTGC	1 cut(s)	3702
BstAUI	TGTACA	1 cut(s)	764
BstBI	TTCGAA	1 cut(s)	3089
BstFNI	CGCG	4 cut(s)	79, 281, 296, 676
BstH2I	RGCGCY	1 cut(s)	461
BstHHI	GCGC	3 cut(s)	296, 460, 3887
BstMAI	GTCTC	7 cut(s)	360, 639, 767, 1467, 1542, 2250, 3104
BstMCI	CGRYCG	1 cut(s)	499
BstNI	CCWGG	3 cut(s)	953, 3402, 3547
BstNSI	RCATGY	3 cut(s)	2104, 2284, 3641
BstSCI	CCNGG	6 cut(s)	151, 484, 951, 1207, 3400, 3545
BstSFI	CTRYAG	4 cut(s)	2223, 2643, 3219, 3282
BstSLI	GKGCMC	2 cut(s)	850, 2485
BstUI	CGCG	4 cut(s)	79, 281, 296, 676
BstV2I	GAAGAC	3 cut(s)	711, 1866, 2427
BstX2I	RGATCY	5 cut(s)	1381, 2725, 2905, 3433, 3460
BstXI	CCANNNNNNTGG	2 cut(s)	664, 3658
BstYI	RGATCY	5 cut(s)	1381, 2725, 2905, 3433, 3460
Bsu15I	ATCGAT	1 cut(s)	1891
Bsu36I	CCTNAGG	2 cut(s)	417, 1638
BsuRI	GGCC	6 cut(s)	156, 409, 484, 2267, 3060, 3400
BsuTUI	ATCGAT	1 cut(s)	1891
BtrI	CACGTC	1 cut(s)	2599
BtsIMutI	CAGTG	4 cut(s)	1347, 3111, 3658, 3676
BveI	ACCTGC	1 cut(s)	6
CaiI	CAGNNNCTG	1 cut(s)	776
CciI	TCATGA	3 cut(s)	1735, 3052, 3718
CfoI	GCGC	3 cut(s)	296, 460, 3887
Cfr10I	RCCGGY	2 cut(s)	268, 340
Cfr13I	GGNCC	8 cut(s)	155, 407, 1552, 2079, 2189, 2265, 2632, 3128
ClaI	ATCGAT	1 cut(s)	1891
CseI	GACGC	4 cut(s)	274, 306, 357, 1913
Csp6I	GTAC	5 cut(s)	765, 911, 1260, 2607, 3228
CviQI	GTAC	5 cut(s)	765, 911, 1260, 2607, 3228
DraI	TTTAAA	1 cut(s)	3520
DraIII	CACNNNGTG	3 cut(s)	1257, 3008, 3653
DrdI	GACNNNNNNGTC	1 cut(s)	2242
DriI	GACNNNNNGTC	1 cut(s)	451
DseDI	GACNNNNNNGTC	1 cut(s)	2242
EaeI	YGGCCR	1 cut(s)	3058
Eam1104I	CTCTTC	2 cut(s)	31, 1463
Eam1105I	GACNNNNNGTC	1 cut(s)	451
EarI	CTCTTC	2 cut(s)	31, 1463
EciI	GGCGGA	2 cut(s)	198, 1353
Ecl136II	GAGCTC	1 cut(s)	285
Eco130I	CCWWGG	5 cut(s)	798, 2240, 2261, 2628, 3395
Eco24I	GRGCYC	3 cut(s)	287, 467, 656
Eco31I	GGTCTC	4 cut(s)	639, 1467, 2250, 3104
Eco32I	GATATC	2 cut(s)	1984, 3526
Eco47I	GGWCC	5 cut(s)	1552, 2079, 2189, 2632, 3128
Eco53kI	GAGCTC	1 cut(s)	285
Eco57I	CTGAAG	7 cut(s)	723, 1238, 1407, 2139, 3444, 3558, 3612
Eco81I	CCTNAGG	2 cut(s)	417, 1638
Eco88I	CYCGRG	3 cut(s)	157, 466, 936
EcoICRI	GAGCTC	1 cut(s)	285
EcoRI	GAATTC	5 cut(s)	898, 1047, 1359, 1528, 3330
EcoRII	CCWGG	3 cut(s)	951, 3400, 3545
EcoRV	GATATC	2 cut(s)	1984, 3526
EcoT14I	CCWWGG	5 cut(s)	798, 2240, 2261, 2628, 3395
EcoT38I	GRGCYC	3 cut(s)	287, 467, 656
ErhI	CCWWGG	5 cut(s)	798, 2240, 2261, 2628, 3395
FalI	AAGNNNNNCTT	2 cut(s)	2964, 2996
FaqI	GGGAC	4 cut(s)	1565, 1690, 2065, 3548
FauI	CCCGC	1 cut(s)	84
FauNDI	CATATG	1 cut(s)	781
FbaI	TGATCA	3 cut(s)	1513, 2200, 3442
FblI	GTMKAC	1 cut(s)	2158
FriOI	GRGCYC	3 cut(s)	287, 467, 656
FspBI	CTAG	9 cut(s)	203, 1263, 1428, 1545, 2241, 2588, 3155, 3483, 3587
GlaI	GCGC	3 cut(s)	295, 459, 3886
GsaI	CCCAGC	4 cut(s)	380, 464, 3820, 3885
GsuI	CTGGAG	3 cut(s)	759, 974, 1161
HaeII	RGCGCY	1 cut(s)	461
HaeIII	GGCC	6 cut(s)	156, 409, 484, 2267, 3060, 3400
HgaI	GACGC	4 cut(s)	274, 306, 357, 1913
HhaI	GCGC	3 cut(s)	296, 460, 3887
Hin1I	GRCGYC	3 cut(s)	266, 368, 1192
Hin6I	GCGC	3 cut(s)	294, 458, 3885
HinP1I	GCGC	3 cut(s)	294, 458, 3885
HincII	GTYRAC	1 cut(s)	3072
HindII	GTYRAC	1 cut(s)	3072
HindIII	AAGCTT	2 cut(s)	1597, 1706
HphI	GGTGA	5 cut(s)	605, 1494, 3511, 3595, 3743
Hpy166II	GTNNAC	8 cut(s)	767, 848, 911, 1009, 1260, 2159, 3072, 3230
Hpy8I	GTNNAC	8 cut(s)	767, 848, 911, 1009, 1260, 2159, 3072, 3230
Hpy99I	CGWCG	3 cut(s)	373, 376, 2471
HpyCH4III	ACNGT	9 cut(s)	1442, 1457, 1767, 2409, 2467, 2566, 2622, 3386, 3716
HpyCH4IV	ACGT	7 cut(s)	1192, 1311, 2010, 2052, 2598, 2800, 3214
HpySE526I	ACGT	7 cut(s)	1192, 1311, 2010, 2052, 2598, 2800, 3214
Hsp92I	GRCGYC	3 cut(s)	266, 368, 1192
HspAI	GCGC	3 cut(s)	294, 458, 3885
Kpn2I	TCCGGA	1 cut(s)	2766
KroI	GCCGGC	1 cut(s)	268
KroNI	GCCGGC	1 cut(s)	270
Ksp22I	TGATCA	3 cut(s)	1513, 2200, 3442
LmnI	GCTCC	5 cut(s)	1447, 1624, 2289, 2587, 3479
MaeI	CTAG	9 cut(s)	203, 1263, 1428, 1545, 2241, 2588, 3155, 3483, 3587
MaeII	ACGT	7 cut(s)	1192, 1311, 2010, 2052, 2598, 2800, 3214
MaeIII	GTNAC	7 cut(s)	678, 731, 2035, 2072, 2801, 3596, 3653
MflI	RGATCY	5 cut(s)	1381, 2725, 2905, 3433, 3460
MhlI	GDGCHC	6 cut(s)	287, 467, 656, 850, 2485, 3586
MmeI	TCCRAC	6 cut(s)	63, 72, 117, 1024, 1868, 3307
MreI	CGCCGGCG	1 cut(s)	268
MroI	TCCGGA	1 cut(s)	2766
MroNI	GCCGGC	1 cut(s)	268
MroXI	GAANNNNTTC	1 cut(s)	1574
MslI	CAYNNNNRTG	1 cut(s)	1235
MspCI	CTTAAG	3 cut(s)	260, 1393, 3685
MspR9I	CCNGG	6 cut(s)	153, 486, 953, 1209, 3402, 3547
Mva1269I	GAATGC	2 cut(s)	1242, 2779
MvaI	CCWGG	3 cut(s)	953, 3402, 3547
MvnI	CGCG	4 cut(s)	79, 281, 296, 676
NaeI	GCCGGC	1 cut(s)	270
NciI	CCSGG	3 cut(s)	153, 486, 1209
NdeI	CATATG	1 cut(s)	781
NgoMIV	GCCGGC	1 cut(s)	268
NlaIV	GGNNCC	9 cut(s)	91, 339, 408, 669, 1383, 1553, 1842, 2081, 3246
NmuCI	GTSAC	5 cut(s)	731, 2035, 2072, 2801, 3653
NspI	RCATGY	3 cut(s)	2104, 2284, 3641
NspV	TTCGAA	1 cut(s)	3089
PaeR7I	CTCGAG	1 cut(s)	466
PagI	TCATGA	3 cut(s)	1735, 3052, 3718
PciI	ACATGT	1 cut(s)	3637
PcsI	WCGNNNNNNNCGW	2 cut(s)	155, 293
PctI	GAATGC	2 cut(s)	1242, 2779
PdiI	GCCGGC	1 cut(s)	270
PdmI	GAANNNNTTC	1 cut(s)	1574
Ple19I	CGATCG	1 cut(s)	499
PscI	ACATGT	1 cut(s)	3637
Psp124BI	GAGCTC	1 cut(s)	287
Psp1406I	AACGTT	1 cut(s)	3214
Psp6I	CCWGG	3 cut(s)	951, 3400, 3545
PspFI	CCCAGC	4 cut(s)	376, 460, 3816, 3881
PspGI	CCWGG	3 cut(s)	951, 3400, 3545
PspN4I	GGNNCC	9 cut(s)	91, 339, 408, 669, 1383, 1553, 1842, 2081, 3246
PspPI	GGNCC	8 cut(s)	155, 407, 1552, 2079, 2189, 2265, 2632, 3128
PspXI	VCTCGAGB	1 cut(s)	466
PstI	CTGCAG	3 cut(s)	2227, 3223, 3286
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	5 cut(s)	1381, 2725, 2905, 3433, 3460
PvuI	CGATCG	1 cut(s)	499
RsaI	GTAC	5 cut(s)	766, 912, 1261, 2608, 3229
RsaNI	GTAC	5 cut(s)	765, 911, 1260, 2607, 3228
RseI	CAYNNNNRTG	1 cut(s)	1235
SacI	GAGCTC	1 cut(s)	287
Sau96I	GGNCC	8 cut(s)	155, 407, 1552, 2079, 2189, 2265, 2632, 3128
ScrFI	CCNGG	6 cut(s)	153, 486, 953, 1209, 3402, 3547
SduI	GDGCHC	6 cut(s)	287, 467, 656, 850, 2485, 3586
SfcI	CTRYAG	4 cut(s)	2223, 2643, 3219, 3282
Sfr274I	CTCGAG	1 cut(s)	466
SfuI	TTCGAA	1 cut(s)	3089
SgrAI	CRCCGGYG	1 cut(s)	268
SinI	GGWCC	5 cut(s)	1552, 2079, 2189, 2632, 3128
SlaI	CTCGAG	1 cut(s)	466
SmiMI	CAYNNNNRTG	1 cut(s)	1235
SmlI	CTYRAG	7 cut(s)	260, 466, 1094, 1393, 2425, 3685, 3706
SmoI	CTYRAG	7 cut(s)	260, 466, 1094, 1393, 2425, 3685, 3706
SspI	AATATT	2 cut(s)	3376, 3391
SspMI	CTAG	9 cut(s)	203, 1263, 1428, 1545, 2241, 2588, 3155, 3483, 3587
SstI	GAGCTC	1 cut(s)	287
StyD4I	CCNGG	6 cut(s)	151, 484, 951, 1207, 3400, 3545
StyI	CCWWGG	5 cut(s)	798, 2240, 2261, 2628, 3395
TaaI	ACNGT	9 cut(s)	1442, 1457, 1767, 2409, 2467, 2566, 2622, 3386, 3716
TaiI	ACGT	7 cut(s)	1195, 1314, 2013, 2055, 2601, 2803, 3217
TaqII	GACCGA	2 cut(s)	2054, 2649
TatI	WGTACW	2 cut(s)	764, 1259
TauI	GCSGC	4 cut(s)	177, 180, 183, 347
TscAI	CASTG	4 cut(s)	1354, 3118, 3658, 3676
TseFI	GTSAC	5 cut(s)	731, 2035, 2072, 2801, 3653
Tsp45I	GTSAC	5 cut(s)	731, 2035, 2072, 2801, 3653
TspDTI	ATGAA	9 cut(s)	906, 1234, 1320, 2348, 2352, 2589, 3005, 3180, 3750
TspGWI	ACGGA	2 cut(s)	2913, 3858
TspRI	CASTG	4 cut(s)	1354, 3118, 3658, 3676
Vha464I	CTTAAG	3 cut(s)	260, 1393, 3685
VneI	GTGCAC	1 cut(s)	846
VpaK11BI	GGWCC	5 cut(s)	1552, 2079, 2189, 2632, 3128
XbaI	TCTAGA	1 cut(s)	3586
XceI	RCATGY	3 cut(s)	2104, 2284, 3641
XhoI	CTCGAG	1 cut(s)	466
XmaJI	CCTAGG	1 cut(s)	2240
XmiI	GTMKAC	1 cut(s)	2158
XmnI	GAANNNNTTC	1 cut(s)	1574
XspI	CTAG	9 cut(s)	203, 1263, 1428, 1545, 2241, 2588, 3155, 3483, 3587
ZraI	GACGTC	1 cut(s)	1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.