MD06G1233500.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003989
Physical Location & Seq
Reverse (-)
36463094 .. 36465228
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1233500.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCTATTCCTTGTAAAAATTGCAGGACCTCTATGTTTTTCTTGATCCTCAGTTTTGGCCTGTGTCTGGTAATTACATCTGCGGAGCTTCAGAGATTCGACCACCCCACCAAACAAGATGGATCACTCAGTTTCTTGGTGGTTGGGGACTGGGGAAGAAGAGGAGATTTCAACCAATCTCAAGTTGCCCTTCAGATGGGAAGGATTGGAGAGAAACTTGACATAGATTTCGTGGTGTCGACGGGAGATAACTTTTACGACAATGGACTCACCAGCGAGCATGACACAGCATTTGAAGAGTCGTTTACCAAAATCTATACAGAAAAGAGCCTGCAGAAGCAGTGGTATAGCGATAAGTTTCTTCTGCGAGGATTGTTGGGAAACCATGATTACAGGGGAGATGCAGAGGCTCAGCTGAGCCCCCTTCTGCGGAAAATCGATAGCAGATGGCTTTGCTTGAGGTCTTTCATTGTGAATACAGAATTAGCAGAGCTTTTCTTCGTGGACACCACGCCCTTGGTGGATAAGTACTTCACAAACGCAGACGAACATACTTACGATTGGCGCGGCATTGCCCCTCGGAAGGCTTATATTGCAAACTTGTTGAAGGATGTGGAGTTGGCATTGAAGAAATCCTCTGCAAAGTGGAAGATTGTTGTTGGACACCATGCAATTAGAAGTGTGGGACATCATGGTGACACACAGGAACTTACAAACCAGCTTCTCCCAATCCTTCGGGTCAATGACGTCGATTTTTACATGAACGGGCACGATCATTGCCTAGAACACGTTAGTGACTTGGAGAGCCCGATACAATTTCTAACAAGCGGAGCAGGGTCTAAAGCATGGAGGGGAGATGTTAAGGGGCTGAACAAAGAAGGGCTCAACTTCTTTTATGATGGTCAAGGCTTCATGTCTGTGAAGTTGAATCCCACAGATGCAGAGATTGCATTTTATGATGTTTATGGCAAGGTTTTACATAGACTGAATGCTTCCAAGCAGCTTCACCCCTCCATATAA

Protein Analysis

340

Amino Acids

38.5

Weight (kDa)

6.11

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 44 - 260 1e-17 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 750
AccI GTMKAC 1 cut(s) 239
AccII CGCG 1 cut(s) 567
AciI CCGC 4 cut(s) 83, 430, 567, 828
AclWI GGATC 2 cut(s) 40, 130
AcuI CTGAAG 2 cut(s) 74, 176
AcyI GRCGYC 1 cut(s) 747
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 4 cut(s) 67, 196, 429, 583
AflIII ACRYGT 1 cut(s) 787
AgsI TTSAA 5 cut(s) 172, 296, 607, 628, 928
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 5 cut(s) 88, 415, 493, 721, 1003
AluI AGCT 5 cut(s) 88, 415, 493, 721, 1003
AlwI GGATC 2 cut(s) 40, 130
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 1000
ArsI GACNNNNNNTTYG 4 cut(s) 212, 244, 275, 307
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 3 cut(s) 262, 707, 998
AvaII GGWCC 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 771
BanII GRGCYC 3 cut(s) 422, 809, 885
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 4 cut(s) 113, 190, 441, 893
BfaI CTAG 1 cut(s) 782
BfmI CTRYAG 1 cut(s) 332
BisI GCNGC 2 cut(s) 568, 1001
BlpI GCTNAGC 2 cut(s) 411, 416
BlsI GCNGC 2 cut(s) 569, 1002
BmcAI AGTACT 1 cut(s) 530
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 391, 928
BmuI ACTGGG 1 cut(s) 160
Bpu1102I GCTNAGC 2 cut(s) 411, 416
BpuEI CTTGAG 2 cut(s) 165, 478
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 747
BsaJI CCNNGG 2 cut(s) 516, 578
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 196, 429, 583
Bse1I ACTGG 1 cut(s) 155
Bse3DI GCAATG 2 cut(s) 570, 775
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 516, 578
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 4 cut(s) 67, 196, 429, 583
BseMI GCAATG 2 cut(s) 570, 775
BseMII CTCAG 4 cut(s) 64, 142, 407, 425
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 1 cut(s) 1012
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 60
BshVI ATCGAT 1 cut(s) 438
BslFI GGGAC 2 cut(s) 161, 699
BslI CCNNNNNNNGG 4 cut(s) 67, 196, 429, 583
BsmFI GGGAC 2 cut(s) 161, 699
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 1 cut(s) 60
Bsp1286I GDGCHC 4 cut(s) 422, 771, 809, 885
Bsp143I GATC 3 cut(s) 45, 122, 772
Bsp1720I GCTNAGC 2 cut(s) 411, 416
BspACI CCGC 4 cut(s) 83, 430, 567, 828
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 4 cut(s) 63, 141, 408, 424
BspDI ATCGAT 1 cut(s) 438
BspFNI CGCG 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 2 cut(s) 40, 130
BsrDI GCAATG 2 cut(s) 570, 775
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 516, 578
BssMI GATC 3 cut(s) 45, 122, 772
BssNI GRCGYC 1 cut(s) 747
BssT1I CCWWGG 1 cut(s) 516
Bst6I CTCTTC 2 cut(s) 154, 291
BstACI GRCGYC 1 cut(s) 747
BstAPI GCANNNNNTGC 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 278, 332
BstDEI CTNAG 4 cut(s) 50, 128, 411, 416
BstF5I GGATG 1 cut(s) 616
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 3 cut(s) 48, 125, 775
BstMBI GATC 3 cut(s) 45, 122, 772
BstMWI GCNNNNNNNGC 2 cut(s) 593, 947
BstSFI CTRYAG 1 cut(s) 332
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 1 cut(s) 1012
BstXI CCANNNNNNTGG 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 438
BsuRI GGCC 1 cut(s) 60
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 1 cut(s) 616
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 2 cut(s) 278, 332
CfoI GCGC 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 27
ClaI ATCGAT 1 cut(s) 438
Csp6I GTAC 1 cut(s) 529
CviAII CATG 7 cut(s) 281, 386, 668, 692, 760, 846, 913
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 4 cut(s) 50, 128, 411, 416
DpnI GATC 3 cut(s) 47, 124, 774
DpnII GATC 3 cut(s) 45, 122, 772
Eam1104I CTCTTC 2 cut(s) 154, 291
EarI CTCTTC 2 cut(s) 154, 291
Eco130I CCWWGG 1 cut(s) 516
Eco24I GRGCYC 3 cut(s) 422, 809, 885
Eco47I GGWCC 1 cut(s) 27
Eco57I CTGAAG 2 cut(s) 74, 176
EcoO109I RGGNCCY 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 516
EcoT38I GRGCYC 3 cut(s) 422, 809, 885
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 7 cut(s) 284, 389, 671, 695, 763, 849, 916
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 2 cut(s) 161, 699
FatI CATG 7 cut(s) 280, 385, 667, 691, 759, 845, 912
FblI GTMKAC 1 cut(s) 239
Fnu4HI GCNGC 2 cut(s) 568, 1001
FokI GGATG 1 cut(s) 623
FriOI GRGCYC 3 cut(s) 422, 809, 885
Fsp4HI GCNGC 2 cut(s) 568, 1001
FspBI CTAG 1 cut(s) 782
GlaI GCGC 1 cut(s) 566
GluI GCNGC 2 cut(s) 568, 1001
HaeIII GGCC 1 cut(s) 60
HhaI GCGC 1 cut(s) 567
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 7 cut(s) 284, 389, 671, 695, 763, 849, 916
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 4 cut(s) 96, 267, 299, 928
HphI GGTGA 3 cut(s) 262, 707, 998
Hpy166II GTNNAC 3 cut(s) 240, 306, 505
Hpy188I TCNGA 3 cut(s) 93, 195, 582
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 3 cut(s) 240, 306, 505
Hpy99I CGWCG 2 cut(s) 244, 752
HpyAV CCTTC 7 cut(s) 195, 200, 434, 577, 601, 743, 872
HpyCH4IV ACGT 2 cut(s) 747, 789
HpyCH4V TGCA 8 cut(s) 24, 334, 404, 596, 641, 671, 941, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 947
HpyF3I CTNAG 4 cut(s) 50, 128, 411, 416
HpySE526I ACGT 2 cut(s) 747, 789
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 7 cut(s) 284, 389, 671, 695, 763, 849, 916
HspAI GCGC 1 cut(s) 565
Kzo9I GATC 3 cut(s) 45, 122, 772
LmnI GCTCC 2 cut(s) 85, 830
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 2 cut(s) 391, 928
MaeI CTAG 1 cut(s) 782
MaeII ACGT 2 cut(s) 747, 789
MaeIII GTNAC 2 cut(s) 695, 794
MalI GATC 3 cut(s) 47, 124, 774
MboI GATC 3 cut(s) 45, 122, 772
MboII GAAGA 7 cut(s) 168, 171, 308, 353, 490, 640, 661
MhlI GDGCHC 4 cut(s) 422, 771, 809, 885
MluCI AATT 5 cut(s) 19, 72, 482, 672, 815
MlyI GAGTC 2 cut(s) 261, 308
MmeI TCCRAC 1 cut(s) 640
MnlI CCTC 9 cut(s) 40, 59, 155, 362, 400, 453, 588, 646, 843
MseI TTAA 1 cut(s) 861
MslI CAYNNNNRTG 3 cut(s) 693, 792, 917
MspA1I CMGCKG 1 cut(s) 415
Mva1269I GAATGC 1 cut(s) 994
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 2 cut(s) 593, 947
NdeII GATC 3 cut(s) 45, 122, 772
NlaIII CATG 7 cut(s) 284, 389, 671, 695, 763, 849, 916
NmuCI GTSAC 2 cut(s) 695, 794
OliI CACNNNNGTG 1 cut(s) 792
PctI GAATGC 1 cut(s) 994
PfeI GAWTC 2 cut(s) 96, 928
PkrI GCNGC 2 cut(s) 569, 1002
PleI GAGTC 2 cut(s) 261, 307
PpsI GAGTC 2 cut(s) 261, 307
PpuMI RGGWCCY 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 27
PspPI GGNCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 27
PstI CTGCAG 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 3 cut(s) 693, 792, 917
SalI GTCGAC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 2 cut(s) 568, 1001
Sau3AI GATC 3 cut(s) 45, 122, 772
Sau96I GGNCC 1 cut(s) 27
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 2 cut(s) 261, 308
SduI GDGCHC 4 cut(s) 422, 771, 809, 885
SfaNI GCATC 2 cut(s) 391, 928
SfcI CTRYAG 1 cut(s) 332
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 3 cut(s) 693, 792, 917
SmlI CTYRAG 2 cut(s) 180, 457
SmoI CTYRAG 2 cut(s) 180, 457
Sse9I AATT 5 cut(s) 19, 72, 482, 672, 815
SsiI CCGC 4 cut(s) 83, 430, 567, 828
SspMI CTAG 1 cut(s) 782
StyI CCWWGG 1 cut(s) 516
TaiI ACGT 2 cut(s) 750, 792
TaqI TCGA 4 cut(s) 99, 239, 438, 750
TasI AATT 5 cut(s) 19, 72, 482, 672, 815
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 2 cut(s) 96, 928
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 2 cut(s) 695, 794
TseI GCWGC 1 cut(s) 1000
Tsp45I GTSAC 2 cut(s) 695, 794
TspDTI ATGAA 3 cut(s) 457, 776, 901
TspRI CASTG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 239
XspI CTAG 1 cut(s) 782
ZraI GACGTC 1 cut(s) 748
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.