MD16G1013500.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003989
Physical Location & Seq
Forward (+)
1047638 .. 1049218
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1013500.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGGTTAGCAGCTCAGCCATGGCAAAACCCCCATTTGCTTCACGCCTTCATCTGGTGTGGGTGTTCTGCATTGCTATTATTTCTTCTTTAGCTGTTTCCACTTCTTTTGCAGAGCTTCAGAGATTGGAACACGCAGCAAAAAGAGATGATGGGTCGCTTAGCTTTTTGGTGGTTGGAGATTGGGGCAGAAGAGGGTTATATAACCAATCCAAAGTTGCTCTACAGATGGGAAGGATTGGAGAGCAATTAGATATAGATTTTGTCGTATCCACTGGGGATAATTTTTACGGCGATGGATTGAAAAATGTAAATGATCCAGCATTTTTGGAGTCCTTTAAAAATATTTACACAGCTAAGAGCTTGCAAAAACAGTGGTATAGTGTTTTAGGAAATCATGATTATAGGGGTAATATTGAGGCACAATTAAGCCCTGCCCTCCGAAAGATCGATAGTCGATGGTTTTGCCAGAGATCCTTTATTGTTAATGCAGGAATTGCGGAATTCTTTTTTGTGGACACCACTCCATTTGTGAACAAATACTTGGTCGACAAAGACCACAATTATGATTGGCGAGGCGTGTCTCCGCGGGCAACTTACCTCGCTGACCTTCTACAGGATTTGAAACAAGCATTGAATAAATCAAGTGCAAAGTGGAAGATAGTGGTTGGTCACCATGCAATCAGAAGTGCTGGGCATCATGGCGACACTCCGGAACTCATAAGTCTCCTTTTACCAATGCTTAAGGCTTATAATGTTGATATGTACATGAATGGACACGACCATTGTTTGGAGCACATTAGTAGCTATTTAACAAGTGGGGCAGGTTCAAAAGCATGGAGGGGTGATATTAAGAGATACAACAATCGAGCCATGAAATTCTACTATGATGGTCAAGGTTTCATGTCCGTGAAGCTGGCTGGATCGGATGCTGAAATTGCATTCTATGATGTTTTAGGCAGGGTTTTGTATACATGGAAGGCTTCTAAGCAGCTTCACTCGACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko04142 ko04380 ko05323 map00740 map01100 map04142 map04380 map05323
Pfam Domains
Protein Families
Metallophos

Protein Analysis

336

Amino Acids

37.8

Weight (kDa)

9.02

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 54 - 262 2.3e-19 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 812
AccB7I CCANNNNNTGG 1 cut(s) 787
AccI GTMKAC 2 cut(s) 546, 968
AccII CGCG 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 709
AciI CCGC 3 cut(s) 497, 584, 586
AclWI GGATC 3 cut(s) 308, 465, 928
AcsI RAATTY 2 cut(s) 500, 875
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 3 cut(s) 52, 613, 787
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 4 cut(s) 301, 622, 634, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AluBI AGCT 9 cut(s) 12, 92, 115, 162, 353, 360, 804, 913, 991
AluI AGCT 9 cut(s) 12, 92, 115, 162, 353, 360, 804, 913, 991
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 2 cut(s) 585, 728
AlwI GGATC 3 cut(s) 308, 465, 928
Aor13HI TCCGGA 1 cut(s) 709
ApeKI GCWGC 3 cut(s) 9, 134, 988
ApoI RAATTY 2 cut(s) 500, 875
ArsI GACNNNNNNTTYG 2 cut(s) 528, 560
AsuHPI GGTGA 2 cut(s) 662, 854
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 3 cut(s) 21, 146, 1000
BccI CCATC 5 cut(s) 143, 220, 287, 450, 881
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 585, 728
BfmI CTRYAG 2 cut(s) 221, 611
BfrI CTTAAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 3 cut(s) 10, 135, 989
BlpI GCTNAGC 2 cut(s) 13, 158
BlsI GCNGC 3 cut(s) 11, 136, 990
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 703, 916
BmuI ACTGGG 1 cut(s) 282
Bpu1102I GCTNAGC 2 cut(s) 13, 158
Bsa29I ATCGAT 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 18, 584
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 613, 787
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 69
BseAI TCCGGA 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 18, 584
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 3 cut(s) 52, 613, 787
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 3 cut(s) 21, 146, 1000
BseYI CCCAGC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 586
BshVI ATCGAT 1 cut(s) 447
BsiHKAI GWGCWC 1 cut(s) 795
BsiSI CCGG 1 cut(s) 710
BslI CCNNNNNNNGG 3 cut(s) 52, 613, 787
BsmAI GTCTC 2 cut(s) 585, 728
BsmI GAATGC 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 795
Bsp13I TCCGGA 1 cut(s) 709
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 4 cut(s) 313, 444, 470, 920
Bsp1720I GCTNAGC 2 cut(s) 13, 158
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 497, 584, 586
BspCNI CTCAG 1 cut(s) 26
BspDI ATCGAT 1 cut(s) 447
BspEI TCCGGA 1 cut(s) 709
BspFNI CGCG 1 cut(s) 586
BspHI TCATGA 1 cut(s) 394
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 3 cut(s) 308, 465, 928
BspTI CTTAAG 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 69
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 18, 584
BssMI GATC 4 cut(s) 313, 444, 470, 920
BssNAI GTATAC 1 cut(s) 969
BssT1I CCWWGG 1 cut(s) 18
Bst1107I GTATAC 1 cut(s) 969
Bst4CI ACNGT 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 3 cut(s) 362, 588, 915
BstDEI CTNAG 4 cut(s) 13, 158, 354, 984
BstDSI CCRYGG 2 cut(s) 18, 584
BstEII GGTNACC 1 cut(s) 668
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 1 cut(s) 931
BstFNI CGCG 1 cut(s) 586
BstKTI GATC 4 cut(s) 316, 447, 473, 923
BstMAI GTCTC 2 cut(s) 585, 728
BstMBI GATC 4 cut(s) 313, 444, 470, 920
BstMWI GCNNNNNNNGC 2 cut(s) 494, 935
BstPI GGTNACC 1 cut(s) 668
BstSFI CTRYAG 2 cut(s) 221, 611
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 3 cut(s) 21, 146, 1000
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BstZ17I GTATAC 1 cut(s) 969
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 277
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 2 cut(s) 18, 584
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 2 cut(s) 270, 377
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 3 cut(s) 362, 588, 915
CciI TCATGA 1 cut(s) 394
Cfr42I CCGCGG 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 763
CspCI CAANNNNNGTGG 6 cut(s) 88, 123, 353, 388, 508, 543
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 4 cut(s) 13, 158, 354, 984
DpnI GATC 4 cut(s) 315, 446, 472, 922
DpnII GATC 4 cut(s) 313, 444, 470, 920
DraI TTTAAA 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 101
Eco91I GGTNACC 1 cut(s) 668
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO65I GGTNACC 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FauI CCCGC 1 cut(s) 579
FblI GTMKAC 2 cut(s) 546, 968
Fnu4HI GCNGC 3 cut(s) 10, 135, 989
FokI GGATG 1 cut(s) 938
Fsp4HI GCNGC 3 cut(s) 10, 135, 989
GluI GCNGC 3 cut(s) 10, 135, 989
GsaI CCCAGC 1 cut(s) 693
HapII CCGG 1 cut(s) 710
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 1 cut(s) 329
HpaII CCGG 1 cut(s) 710
HphI GGTGA 2 cut(s) 662, 854
Hpy166II GTNNAC 4 cut(s) 514, 532, 547, 969
Hpy188I TCNGA 4 cut(s) 120, 440, 683, 925
Hpy188III TCNNGA 2 cut(s) 395, 710
Hpy8I GTNNAC 4 cut(s) 514, 532, 547, 969
HpyAV CCTTC 4 cut(s) 56, 225, 617, 970
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 69, 110, 364, 488, 647, 677, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 935
HpyF3I CTNAG 4 cut(s) 13, 158, 354, 984
Kpn2I TCCGGA 1 cut(s) 709
KspI CCGCGG 1 cut(s) 587
Kzo9I GATC 4 cut(s) 313, 444, 470, 920
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 3 cut(s) 21, 146, 1000
LweI GCATC 2 cut(s) 703, 916
MaeIII GTNAC 1 cut(s) 668
MalI GATC 4 cut(s) 315, 446, 472, 922
MboI GATC 4 cut(s) 313, 444, 470, 920
MboII GAAGA 3 cut(s) 75, 201, 667
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 875, 933
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 6 cut(s) 185, 409, 446, 566, 608, 831
MroI TCCGGA 1 cut(s) 709
MseI TTAA 6 cut(s) 336, 425, 483, 741, 809, 849
MslI CAYNNNNRTG 2 cut(s) 561, 905
MspA1I CMGCKG 1 cut(s) 586
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 938
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 494, 935
NcoI CCATGG 1 cut(s) 18
NdeII GATC 4 cut(s) 313, 444, 470, 920
NmuCI GTSAC 1 cut(s) 668
PagI TCATGA 1 cut(s) 394
PctI GAATGC 1 cut(s) 938
PflMI CCANNNNNTGG 1 cut(s) 787
PkrI GCNGC 3 cut(s) 11, 136, 990
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PsiI TTATAA 1 cut(s) 750
PspEI GGTNACC 1 cut(s) 668
PspFI CCCAGC 1 cut(s) 689
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
RseI CAYNNNNRTG 2 cut(s) 561, 905
SacII CCGCGG 1 cut(s) 587
SalI GTCGAC 1 cut(s) 545
SaqAI TTAA 6 cut(s) 336, 425, 483, 741, 809, 849
SatI GCNGC 3 cut(s) 10, 135, 989
Sau3AI GATC 4 cut(s) 313, 444, 470, 920
SchI GAGTC 1 cut(s) 338
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 2 cut(s) 703, 916
SfcI CTRYAG 2 cut(s) 221, 611
Sfr303I CCGCGG 1 cut(s) 587
SgrBI CCGCGG 1 cut(s) 587
SmiMI CAYNNNNRTG 2 cut(s) 561, 905
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 875, 933
SsiI CCGC 3 cut(s) 497, 584, 586
SspI AATATT 2 cut(s) 343, 412
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 372
TaqI TCGA 5 cut(s) 447, 454, 546, 865, 998
TasI AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 875, 933
TatI WGTACW 1 cut(s) 762
Tru1I TTAA 6 cut(s) 336, 425, 483, 741, 809, 849
Tru9I TTAA 6 cut(s) 336, 425, 483, 741, 809, 849
TscAI CASTG 2 cut(s) 277, 377
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 3 cut(s) 9, 134, 988
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 4 cut(s) 38, 782, 887, 889
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 2 cut(s) 277, 377
Van91I CCANNNNNTGG 1 cut(s) 787
Vha464I CTTAAG 1 cut(s) 740
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 2 cut(s) 500, 875
XmiI GTMKAC 2 cut(s) 546, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.