MD07G1010400.v1.1

ATP synthase subunit O

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004768
Physical Location & Seq
Forward (+)
944437 .. 948785
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1010400.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCATTTGCTACTCGAATTCGATCGAGCCTCCCTCTGGTCACCAAGATTCTCAAATCAGATTCGCTCTCTGCCGCCGCCAACGGATCCATCGCCCACCGCTCCGTGCTTTGCCCTACTTTCATCAATTCTGAGCCATCCAGGAACTTTTCTACCGCGCAAGGGAAGAAGGAAGTGAAGGTTCAGGTGCCATTGGTTTTGTTTGGGGGTTCTGGGAACTATGCCTCTGCTTTGTACATTGCTGCAGTAAAAGCTAATGCTTTGGAGAAGGTTGAGTCTGAGATTTTTGCCATTGTTGAGGCTACGAAGAAAAGTCCTACCTTTTCTCAGTTCACAAAGGATTTGTCAGTGCCAGCAGATACTAGAGTGAAGGCAATCGATCAAATTGCAGCTGAAGCTAAATTTTCAGAAATTACAAAGAACTTTTTGGTTGTCTTGGCTCAGAATGGGAGGCTAAGGAACCTTGAAACCATATCAAAGAGATTTGCGGAGTTAACCATGGCACATAAGGGAGAAGTGAAAGCTATTGTCACATCTGTCATTCCCCTTCCTGCTGAAGAGGAGAAAGAATTGAAGGAGACACTGCAGGAATTAATTGGACAAGGGAAGAAGGTCATTCTTGAACAGAAGATTGATCCTAGTATATTGGGTGGGCTCGTGATAGAGTTTGATAAGAAGGTATTTGACATGTCCATCAAGACTCGGGCACGCCAGATGGAGAGGTATTTGCGGGAACCCGTAAACTTTGACAACCTCTAA

Protein Analysis

253

Amino Acids

27.74

Weight (kDa)

9.38

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OSCP PF00213 72 - 243 1.5e-44 ATP synthase delta (OSCP) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 187
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 1 cut(s) 158
AciI CCGC 6 cut(s) 75, 78, 100, 156, 488, 730
AclWI GGATC 3 cut(s) 81, 94, 629
AcsI RAATTY 2 cut(s) 18, 401
AcuI CTGAAG 2 cut(s) 414, 576
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 37, 162
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 3 cut(s) 467, 574, 623
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 4 cut(s) 254, 392, 398, 524
AluI AGCT 4 cut(s) 254, 392, 398, 524
Alw26I GTCTC 1 cut(s) 572
AlwI GGATC 3 cut(s) 81, 94, 629
Ama87I CYCGRG 1 cut(s) 702
ApeKI GCWGC 2 cut(s) 242, 389
ApoI RAATTY 2 cut(s) 18, 401
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AseI ATTAAT 1 cut(s) 593
AspLEI GCGC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 709
BaeI ACNNNNGTAYC 2 cut(s) 351, 384
BamHI GGATCC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 657
BauI CACGAG 1 cut(s) 656
BbvI GCAGC 2 cut(s) 229, 401
BccI CCATC 4 cut(s) 98, 145, 701, 709
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 2 cut(s) 363, 639
BfmI CTRYAG 2 cut(s) 243, 584
BisI GCNGC 4 cut(s) 75, 78, 243, 390
BlsI GCNGC 4 cut(s) 76, 79, 244, 391
Bme1390I CCNGG 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 702
BmiI GGNNCC 4 cut(s) 88, 189, 461, 735
BmrFI CCNGG 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 19, 51
Bpu10I CCTNAGC 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 498
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 162
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 142
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 2 cut(s) 37, 162
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 4 cut(s) 123, 270, 341, 455
BseRI GAGGAG 1 cut(s) 575
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 2 cut(s) 229, 401
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 702
BslI CCNNNNNNNGG 2 cut(s) 37, 162
BsmAI GTCTC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 657, 709
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 4 cut(s) 23, 86, 379, 634
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 6 cut(s) 75, 78, 100, 156, 488, 730
BspCNI CTCAG 4 cut(s) 124, 271, 340, 454
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 4 cut(s) 88, 189, 461, 735
BspMAI CTGCAG 2 cut(s) 247, 588
BspPI GGATC 3 cut(s) 81, 94, 629
BspT107I GGYRCC 1 cut(s) 187
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 4 cut(s) 23, 86, 379, 634
BssSI CACGAG 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 552
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 354, 709
BstDEI CTNAG 5 cut(s) 132, 279, 327, 441, 455
BstDSI CCRYGG 1 cut(s) 498
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 1 cut(s) 137
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 4 cut(s) 26, 89, 382, 637
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 4 cut(s) 23, 86, 379, 634
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 2 cut(s) 251, 395
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 691
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 2 cut(s) 243, 584
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 2 cut(s) 229, 401
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 378
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 498
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 137
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 2 cut(s) 354, 581
Cac8I GCNNGC 2 cut(s) 354, 709
CfoI GCGC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 499, 688
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 5 cut(s) 132, 279, 327, 441, 455
DpnI GATC 4 cut(s) 25, 88, 381, 636
DpnII GATC 4 cut(s) 23, 86, 379, 634
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 657
Eco57I CTGAAG 2 cut(s) 414, 576
Eco88I CYCGRG 1 cut(s) 702
Eco91I GGTNACC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 2 cut(s) 502, 691
FaiI YATR 6 cut(s) 222, 473, 500, 507, 644, 689
FatI CATG 2 cut(s) 498, 687
FauI CCCGC 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 75, 78, 243, 390
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 4 cut(s) 75, 78, 243, 390
FspBI CTAG 2 cut(s) 363, 639
GlaI GCGC 1 cut(s) 159
GluI GCNGC 4 cut(s) 75, 78, 243, 390
HhaI GCGC 1 cut(s) 160
Hin1II CATG 2 cut(s) 502, 691
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HinfI GANTC 4 cut(s) 49, 62, 275, 700
HpaI GTTAAC 1 cut(s) 495
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 3 cut(s) 333, 495, 742
Hpy188I TCNGA 5 cut(s) 61, 133, 280, 409, 444
Hpy188III TCNNGA 3 cut(s) 620, 658, 697
Hpy8I GTNNAC 3 cut(s) 333, 495, 742
HpyAV CCTTC 8 cut(s) 163, 172, 262, 364, 557, 568, 604, 670
HpyCH4V TGCA 3 cut(s) 245, 389, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 395
HpyF3I CTNAG 5 cut(s) 132, 279, 327, 441, 455
Hsp92II CATG 2 cut(s) 502, 691
HspAI GCGC 1 cut(s) 158
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 4 cut(s) 23, 86, 379, 634
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 9 cut(s) 23, 127, 154, 170, 198, 366, 564, 572, 725
Lsp1109I GCAGC 2 cut(s) 229, 401
MaeI CTAG 2 cut(s) 363, 639
MaeIII GTNAC 2 cut(s) 40, 529
MalI GATC 4 cut(s) 25, 88, 381, 636
MbiI CCGCTC 1 cut(s) 102
MboI GATC 4 cut(s) 23, 86, 379, 634
MboII GAAGA 5 cut(s) 178, 319, 569, 619, 640
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 2 cut(s) 657, 709
MluCI AATT 8 cut(s) 18, 127, 384, 401, 411, 569, 590, 594
MlyI GAGTC 2 cut(s) 284, 694
MnlI CCTC 7 cut(s) 41, 45, 235, 292, 444, 553, 714
MseI TTAA 2 cut(s) 494, 593
MspA1I CMGCKG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 251, 395
NcoI CCATGG 1 cut(s) 498
NdeII GATC 4 cut(s) 23, 86, 379, 634
NlaIII CATG 2 cut(s) 502, 691
NlaIV GGNNCC 4 cut(s) 88, 189, 461, 735
NmuCI GTSAC 2 cut(s) 40, 529
NspI RCATGY 1 cut(s) 691
PciI ACATGT 1 cut(s) 687
PfeI GAWTC 2 cut(s) 49, 62
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 4 cut(s) 76, 79, 244, 391
Ple19I CGATCG 1 cut(s) 26
PleI GAGTC 2 cut(s) 283, 694
PpsI GAGTC 2 cut(s) 283, 694
PscI ACATGT 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 140
PspEI GGTNACC 1 cut(s) 40
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 88, 189, 461, 735
PstI CTGCAG 2 cut(s) 247, 588
PsuI RGATCY 1 cut(s) 86
PvuI CGATCG 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 494, 593
SatI GCNGC 4 cut(s) 75, 78, 243, 390
Sau3AI GATC 4 cut(s) 23, 86, 379, 634
SchI GAGTC 2 cut(s) 284, 694
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 2 cut(s) 657, 709
SfcI CTRYAG 2 cut(s) 243, 584
Sse9I AATT 8 cut(s) 18, 127, 384, 401, 411, 569, 590, 594
SsiI CCGC 6 cut(s) 75, 78, 100, 156, 488, 730
SspMI CTAG 2 cut(s) 363, 639
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 498
TaqI TCGA 4 cut(s) 16, 22, 26, 378
TasI AATT 8 cut(s) 18, 127, 384, 401, 411, 569, 590, 594
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 77, 80
TfiI GAWTC 2 cut(s) 49, 62
Tru1I TTAA 2 cut(s) 494, 593
Tru9I TTAA 2 cut(s) 494, 593
TscAI CASTG 2 cut(s) 354, 588
TseFI GTSAC 2 cut(s) 40, 529
TseI GCWGC 2 cut(s) 242, 389
Tsp45I GTSAC 2 cut(s) 40, 529
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 2 cut(s) 94, 99
TspRI CASTG 2 cut(s) 354, 588
VspI ATTAAT 1 cut(s) 593
XapI RAATTY 2 cut(s) 18, 401
XceI RCATGY 1 cut(s) 691
XspI CTAG 2 cut(s) 363, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.